Results The induction of premalignant lesions after 24 weeks and of HCC-like tumours after 42 weeks by DEN in mice was accompanied by significant leucocyte infiltration in the liver and upregulation of distinct intrahepatic chemokines (CCL2, CCL5, CXCL9). Macrophages and CD8 (cytotoxic) T cells were most prominently enriched in tumour-bearing livers, similar to samples from human HCC. Myeloid-derived suppressor cells (MDSC) increased in extrahepatic compartments of DEN-treated mice (bone marrow, spleen). The contribution of immune cell subsets for DEN-induced hepatocarcinogenesis was functionally dissected. In D6−/− mice, which lack the chemokine scavenging receptor D6, hepatic macrophage infiltration was significantly increased, but tumour formation and progression did not differ from that of WT mice. In contrast, progression of hepatic tumours (numbers, diameters, tumour load) was strikingly enhanced in T-/B cell-deficient Rag1−/− mice upon DEN treatment. When mice deficient for B ...
Hepatocellular carcinoma is the most common cancer occurring in males in the world and has an annual incidence worldwide of 1 million. There are variations in its geographical distribution. It is the second most common cancer in Southeast Asia, including Hong Kong and ranks third among males in China ...
Animals. Alfp-Cre (28), Ripk1fl/fl (8), Ripk1fl/D138N (33), Tnfr1fl/fl (29), Faddfl/fl (31), Traddfl/fl (30), and RelAfl/fl (46) mice were reported earlier. Mice were maintained at the specific pathogen-free animal facilities of the Institute for Genetics and the CECAD Research Center, University of Cologne, under a 12-hour dark/12-hour light cycle and were given a regular chow diet (Harlan diet no. 2918 or Prolab Isopro RMH3000 5P76) ad libitum. For obesity-induced DEN carcinogenesis, animals were allowed ad libitum access to either NCD (sniff EF acc. D12450B [l] mod.) or HFD (ssniff EF acc. D12492 [l] mod.) starting at 4 weeks of age. Animals requiring medical attention were provided with appropriate care and excluded from the experiments described. No other exclusion criteria existed.. DEN-induced liver tumorigenesis. To induce liver tumorigenesis, mice were injected i.p. at 2 weeks of age with 25 mg/kg BW DEN (Sigma-Aldrich) and were sacrificed at the age of 32 or 36 weeks using CO2. Acute ...
Guadalupe S. López-Álvarez, Tomasz K. Wojdacz, Claudia M. García-Cuellar, Hugo C. Monroy-Ramírez, Miguel A. Rodríguez-Segura, Ruth A. Pacheco-Rivera, Carlos A. Valencia-Antúnez, Nancy Cervantes-Anaya, Ernesto Soto-Reyes, Verónica R. Vásquez-Garzón, Yesennia Sánchez-Pérez, Saúl Villa-Treviño ...
Pre- and post-initiation modulating effects of green tea ingestion on rat hepatocarcinogenesis Green tea;hepatocarcinogenesis;initiation;GST-P;rats; The purpose of this study was to investigate the effects of green tea ingestion on hepatocarcinogenesis before and after its initiation. Male Sprague-Dawley rats were fed an AIN76A diet with or without green tea. Initiation was induced by a single dose (200 mg/kg) of diethylnitrosamine at week 4 and 0.02% (w/w) 2-acetylaminofluorene was supplied in the diets. The control group had free access to water for 13 weeks (CTR13). Tea infusion was provided from the beginning of the experiment for 13 weeks (PRE13) or from the post-initiation stage until week 13 (POST13). Three other groups (CTR24, PRE24 and POST24) were added to examine the longer-term effects (24 weeks) with the same experimental design. The percentage area of liver sections that were positive for hepatic placental glutathione S-transferase (GST-P), which was used as a marker of preneoplastic
Nitrosamines are carcinogens that require metabolic activation by CYP enzymes in order to exert their carcinogenic effect. Species differences exist in their esophageal carcinogenic potency, with the rat being the most sensitive and the Syrian hamster a resistant species. In the latter, the liver is the main target organ. This difference does not apply to directly acting N-nitroso compounds, suggesting that tissue-specific metabolic activation is involved in hamster esophageal resistance to nitrosamines. We have previously shown that Cytochrome P450 2A3 (CYP2A3) is responsible for N-nitrosodiethylamine activation in the rat esophagus. In order to find a mechanistic explanation for the resistance of hamster esophagus for nitrosamines, we have compared the metabolism of NDEA between esophagus and liver of the hamster. Hamster esophagus is capable of activating NDEA (K-m = 1.02 +/- 0.44 mu M and V-max = 1.96 +/- 0.26 nmol acetaldehyde/min/mg microsomal protein). However, the hamster liver showed a ...
Hepatocellular carcinoma (HCC) is a devastating consequence of chronic inflammatory liver diseases. We recently find that inhibition of TLR4 promotes tumor metastases. We aimed to investigate whether TLR4 activity contributes to HCC initiation and progression in mice. A mouse model of diethylnitrosamine (DEN)-induced HCC was generated with wild type and TLR4 mutant mice and the development and progression of HCC and senescent responses were assessed by morphologic, immunological and biochemical criteria. We found that genetic or pharmacologic blocking of TLR4 increased susceptibility to DEN-induced HCC carcinogenesis and progression, which was indicated by the increases in tumor nodules, tumor volume and animal death. The enhanced HCC associated with a broad-spectrum reduction of immune response to DEN liver injury as indicated by decreases in the liver-infiltrating F4/80+ macrophages, the ASK1/p38 MAPK/NF-κB and IRF3 signaling activities, and the expression of inflammatory cytokines. ...
Here we report further our investigation of the role of BRE in HCC. HCC was chemically-induced in the transgenic (TTR-V5-BRE) and non-transgenic littermates, bred with C57BL/6, by intraperitoneal injection of diethylnitosamine (DEN) at 15 days postnatally. At 8 months after injection, the mice were sacrificed, and livers collected for determination of tumor number and maximal size, and for immunohistochemistry. Parts of each liver sample were also dissected visually into tumor and adjacent normal portions for Western Blot analysis of BRE expression. By comparison between the DEN-treated male transgenic mice (n=12) and non-transgenic littermate controls (n=8), we observed significantly increased tumor size shown by the former (p=0.049, Exact Wilcoxon Rank Sum test), with the median tumor size 2-fold larger than the latter. There was, however, no statistically significant difference between tumor numbers of the two groups. Female C57BL/6 mice are known to be less sensitive to DEN-treated ...
Tumor cells were treated with rabbit antibody to tumor-associated cell surface antigens and tested with erythrocytes coated with antibody specific for the sensitizing rabbit immunoglobulin. The sensitized tumor cells formed rosettes with the indicator cells. By this method, we confirmed that line 1 and line 10 hepatoma cells (from two tumors independently induced by diethylnitrosamine in strain 2 guinea pigs) bear antigens not present on normal liver cells. We also confirmed that line 1 and line 10 cells bear antigenically different tumor-associated cell surface antigens. This method appears simpler than other serological methods for detecting tumor-associated cell surface antigens on tumor cells. Also, this method may be a general one for detecting and enumerating cells bearing surface antigens.. ...
Biochemical and molecular biological approaches in situ have provided compelling evidence for early bioenergetic changes in hepatocarcinogenesis. Hepatocellular neoplasms regularly develop from preneoplastic foci of altered hepatocytes, irrespective of whether they are caused by chemicals, radiation …
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suffered chronic digestive problems: there was a family history of IBS, and Paul noticed that certain foods and stress caused Paul also disliked the group sessions. He was "not a lover of group bloating. Cancer medication had caused weight gain, and he therapies", and "hearing other peoples problems makes me had started to diet. He experienced nightly headaches, which he feel anxious". He often avoided the group by arriving late for his associated with his sleep problems. Memory and concentration appointments; when possible, the NADA specialist treated him in "wavered a bit", but was not considered to be a problem. a separate room. Nevertheless, Paul valued the service, and at the end of treatment, he wrote that it was: Paul had initially felt "suicidal" when diagnosed. His wife had "Comforting to come into a service regularly. A positive focus to helped to put things in perspective, and he had been able to start have regular contact with the therapist." to cope with it. Nevertheless, he felt ...
29.05.2008: Oversiktsartikkel - Prostatakreft er den vanligste kreftformen blant menn i Norge, over 3 600 nye tilfeller i 2005, og representerer en stor medisinsk utfordring (1).
Heavy Meldung, Hillepimpf! Das allerneuste vom stählernden Nachrichtenstand. Ob Ozzy seiner Frau den Kopf abgebissen oder Bruce Dickinson sich beim Kacken den Arm gebrochen hat - hier wird über alles diskutiert, was den Hard Rock-Mudschaheddin interessiert!
23.10.2003: Aktuelt - Behandlingen av pasienter med forhøyet serum-urinsyrenivå eller anfall med urinsyregikt stiller store krav til klinikere, forskere og epidemiologer.
Die Seite wurde neu angelegt: „{, class=wikitable sortable center zebra ,- class=hintergrundfarbe6 ! Elementname ! Symbol ! Nr ! Elemonster ,- align=center , align=left, Wasserstof…") ...
Epilepsi er en krampeforstyrrelse der det inntrer en plutselig, forbigående endring i hjernefunksjonen. Den friske hjernen er bygd opp av millioner av...
Hilt, Bjørn; Heggland, Ingrid; Aas, Oddfrid; Qvenild, Torgunn; Svendsen, Kristin V Hirsch; Syversen, Tore; Melø, Inger; Sletvold, Helge. (2012) Mulige senskader hos tannhelsepersonell etter kvikksølveksponering. Tidsskrift for Den norske legeforening. vol. 14 (132). ...
Liver cirrhosis is a predominant risk factor for hepatocellular carcinoma (HCC). However, the mechanism underlying the progression from cirrhosis to HCC remains unclear. Herein we report the concurrent increase of liver progenitor cells (LPCs) and transforming growth factor-β (TGF-β) in diethylnitrosamine (DEN)-induced rat hepatocarcinogenesis and cirrhotic livers of HCC patients. Using several experimental approaches, including 2-acetylaminofluorene/partial hepatectomy (2-AAF/PHx) and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-elicited murine liver regeneration, we found that activation of LPCs in the absence of TGF-β induction was insufficient to trigger hepatocarcinogenesis. Moreover, a small fraction of LPCs was detected to coexpress tumor initiating cell (T-IC) markers during rat hepatocarcinogenesis and in human HCCs, and TGF-β levels were positively correlated with T-IC marker expression, which indicates a role of TGF-β in T-IC generation. Rat pluripotent LPC-like WB-F344 cells ...
A Weibull analysis is presented of the dose and time relationships for the effects on 4 080 inbred rats of chronic ingestion in the drinking water of 16 different doses of N-nitroso-dimethylamine (NDMA) and of N-nitrosodiethylamine (NDEA). The sites chiefly affected were the liver (by both agents) and the oesophagus (by NDEA only). Since the experiment continued into extreme old age, effects became clearly measurable even at a dose of only 0.01 mg/kg per day, which is an order of magnitude lower than previously achieved. (After only two years of treatment, however, the TD50 doses needed to halve the proportion of tumourless survivors would have been about 0.06 mg/kg per day of NDEA and about 0.12 mg/kg per day of NDMA.) The general pattern of response was that the log probability of remaining tumourless was given by the product of two terms, the first (the Weibull b-value) depending on the dose-rate but not on the duration of exposure, and the second depending not on dose at all but on
KITCHIN K. T. Ornithine Decarboxylase Induction and DNA Damage as Predictive Assay for Potential Carcinogenicity, 137-144, 1991 被引用文献2件 ...
(5R,10R)-4R,8R-dihydroxy-2S,3R:15,16-diepoxycleroda-13(16),17,12S:18,1S-dilactone: a diterpenoid from Tinospora cordifolia with chemopreventive potential in diethylnitrosamine-induced hepatocellular carcinoma rats; structure in first source
This page includes the following topics and synonyms: N-Nitrosodimethylamine, NMDA, Dimethylnitrosamine, N-nitrosodiethylamine, NDEA.
Köhler, A. , Broeg, K. and Bahns, S. (1998): Localisation of a tumor-associated phenotype of Benz-aldehyde Dehydrogenase in liver carcinogenesis of flounder by quantitative histochemistry , Marine environmental research ...
DRH strain rats are a unique experimental model in which to study chemically induced liver carcinogenesis. They have been selected from a closed colony of Donryu rats fed 3′-Me-DAB by sister-brother mating for over 40 generations. Their resistance to hepatocarcinogens has been suggested to be genetically dominant (15) . In the present study, we focused on genetic control in the induction of preneoplastic lesions. Five phenotypic parameters were selected to quantitatively describe the preneoplastic lesions; i.e., the number of GST-P-positive foci, the percentage of liver area occupied by EAF, the size of the foci, and the amounts of GST-P as well as GGT mRNA. Their phenotypic values were mutually closely interrelated [for instance, percentage of liver area occupied by EAF and GST-P mRNA (r = 0.763)], but still they seemed under differential genetic control by combinations of host loci. No single phenotypic parameter was controlled by a single locus. The contributions of the loci to phenotype ...
Hepatocarcinogenesis comprises of multiple, complex steps that occur after liver injury and usually involve several pathways, including telomere dysfunction, cell cycle, WNT/β-catenin signaling, oxidative stress and mitochondria dysfunction, autophagy, apoptosis, and AKT/mTOR signaling. Following liver injury, gene mutations, accumulation of oxidative stress, and local inflammation lead to cell proliferation, differentiation, apoptosis, and necrosis. The persistence of this vicious cycle in turn leads to further gene mutation and dysregulation of pro- and anti-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-10, IL-12, IL-13, IL-18, and transforming growth factor (TGF)-β, resulting in immune escape by means of the NF-κB and inflammasome signaling pathways. In this review, we summarize studies focusing on the roles of hepatocarcinogenesis and the immune system in liver cancer. In addition, we furnish an overview of recent basic and clinical studies to provide a strong foundation to
DeNA Co Ltd and Walt Disney Co said on Friday they would jointly develop mobile social games, marking another step in the Japanese gaming platform operators quest to become a global player.
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De senaste betydande inflödena av syrerikt vatten från Västerhavet skedde under 1993-1994. Syresättningen av djupvattnet ledde till en nyrekrytering av bottendjur till stora områden som då saknade makroskopiskt liv. Under den efterföljande stagnationsperioden har djurlivet åter successivt utarmats och slagits ut, först på de största djupen, sedan allt grundare. Nedan följer en redogörelse för utvecklingen i Egentliga Östersjöns bassänger efter 1994, med vissa tillbakablickar till tidigare år.. ...
Den nye serien, OFYR PRO, ble designet spesielt for hoteller, restauranter og catering. Den har den samme robuste og minimalistiske designen som den vanlige produktlinjen, men er i tillegg utstyrt med praktiske funksjoner for å skape perfekt gjestfrihet og for å lyse opp arrangementet ditt. Med OFYR PRO serien kan du betjene større grupper, og du gir dine kunder en uforglemmelig opplevelse rundt ilden med en fristende kombinasjon av deilig mat og "show cooking ...
Naturwissenschaftlicher Konferenzkalender mit Konferenzen zum Thema Biochemie und Medizinische Chemie, Toxikologie in den Vereinigten Staaten (USA)
Minervas Den is the fourth DLC for BioShock 2 and the second to expand upon the singleplayer. In this case it is a self-contained story in the BioShock 2 universe. The player controls a new character who has teamed up with Dr. Tenenbaum to liberate a Rapture district. It introduces Splicers who can...
Naturwissenschaftlicher Konferenzkalender mit Konferenzen zum Thema Massenspektrometrie, Chromatographie und Kernspinresonanzspektroskopie in den Vereinigten Staaten (USA)
Home » Neueste Physikalisch-Politische Reisen in den Jahren 1788 und 1789 durch die Dacischen und Sarmatischen oder Nördlichen ...
Baggrund: Der er ikke specifikke data for fexofenadin. Fexofenadin er den aktive metabolit af terfenadin, som tidligere var på markedet. Efter moderens indtagelse af terfenadin er udskillelsen af den aktive metabolit målt i modermælk. Den relative vægtjusterede dosis for fexofenadin er 0,71%. Det er ikke sandsynligt, at barnet vil blive eksponeret for klinisk relevante mængder. ...
Lungene er de organene vi puster med. De har en svampliknende oppbygning og er delt opp i mindre lapper. Mens den høyre lungen har tre lapper, har den...
2000 (svensk)Inngår i: Tidskrift för politisk filosofi, ISSN 1402-2710, nr 1, s. 5-30Artikkel i tidsskrift (Fagfellevurdert) Published ...
was konkret die Daten waren... Kann bitte jemand eintragen, was der frühestmögliche An- und spätestmögliche Abreisetermin für die ORGA-Truppe ...
Retroperitoneale Paragangliome sind seltene Tumoren mit den Eigenschaften adrenaler Phäochromozytome. Sie kommen entweder sporadisch oder im Zusammenh
Hepatocellular carcinoma is one of the most common cancers in the modern world and it is prevalent in Asia. Environmental and dietary factors play an major role in the development of many cancers. Dietary modification can also play important role in reducing the risk of cancer. Garlic (Allium sativum) is a well known medicinal herb and a food item that has been used all over the world since pre historic times. Previous studies using cancer cell lines and animal models have indicated that garlic and some of its sulfur compounds are potential anticarcinogens. The present study was carried out to investigate the effects of a therapeutic dose (20mg/kg body wt./day) of garlic on chemically induced hepatocarcinogenesis in normal and hypercholesterolaemic Wistar rats. In the fist phase of this study the inhibitory effects of a therapeutic dose (20mg/kg body wt./day) of garlic on diethylnitrosamine (DEN)- induced neoplasia of the liver were examined in normal male Wistar rats. Medium-term Bio assay ...
We have investigated levels of transcript homologous with glutathione S-transferase P (GST-P; GST 7-7) in tumours and hyperplastic lesions induced in the livers of rats by long-term gavage dosing with diethylnitrosamine (DEN) and 6-p-dimethylaminophenylazobenzothiazole (6BT). Detailed histopathological examination of the livers of the 90 animals used in this study at 6-8 months after initiation of daily dosing revealed that, of the 30 animals treated with carcinogen, 15 had developed tumours or hyperplastic lesions. Of these, 11 were areas of fibrosarcoma/fibrous hyperplasia. The remaining four were hepatocellular carcinomas. Northern blotting of total RNA purified from these tissues revealed the presence of transcripts of 3 and 0.75 kb. Evidence is presented to indicate that the former is a hitherto-undetected precursor of the 3-kbp rat GST-P gene, the latter representing the previously characterized mature GST-P transcript. Large elevations of the 0.75-kb transcript (30-35-fold) were ...
The herbal extract Benja‑ummarit (BU) is a traditional Thai medicine with a putative cancer‑suppressing effect. However, this effect has only been tested in vitro in human hepatocarcinoma cell lines. The present study determined the efficacy of a BU extract to treat hepatocellular carcinoma (HCC) in rats in vivo and established its anti‑angiogenic and anti‑proliferative properties. The BU extract was prepared in 95% ethanol and its composition determined using liquid chromatography‑mass spectrometry. HCC was induced in Wistar rats by an injection of diethylnitrosamine (DEN), followed 2 weeks later by injections of thioacetamide (TAA) thrice weekly for 4 weeks. Following 2 months, the DEN‑TAA‑treated rats were divided into 6 groups that were treated orally for another 2 months with: i) No treatment; ii) vehicle; iii) 30 mg/kg sorafenib (SF); iv) 1 mg/kg BU; v) 10 mg/kg BU; or vi) 50 mg/kg BU. Liver samples were collected for gross morphological, histological, reverse ...
To explore the underlying mechanisms for the protective effects of garlic oil (GO) against nitrosodiethylamine (NDEA)-induced hepatocarcinoma, 60 male Wistar rats were randomized into 4 groups (...
Carcinogen-induced lesions were identified by use of the known markers of hepatocarcinogenesis adenosintriphosphatase and ã-glutamyl transpeptidase. While the GSTs and mEHb were increased in all preneoplastic and neoplastic lesions, the levels of the individual cyt. P-450 isoenzymes were characteristically different from each other. In many of the early ATPase deficient islets PB1 was elevated, whereas the content of the other cyt P-450 forms and NADPH-cytochrome P-450 reductase was either unchanged or slightly lowered. At later stages of hepatocarcinogenesis PB1 returned to the levels of the surrounding tissue, while the other cyt. P-450 isoenzymes were decreased, the most prominent reduction being found in MC1. In neoplastic nodules all cyt. P-450s and NADPH-cyt. P-450 reductase were diminuished, some of them dramatically ...
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Det har de senaste tio åren skett omfattande förändringar på den svenska telemarknaden för fast telefoni till följd av en avreglering. Denna uppsts syftar till att undersöka i vilken utsträckning avregleringen förbättrat marknadens funktionssätt. Den vill besvara om marknaden utvecklat billigare och alternativa tjänster och hur marknaden svarat på den nya situationen. Det empiriska undelaget för uppsatsen är primärt och sekundärt material om prissättning, marknadsfördelning och kunders uppfattning om marknaden. Avslutningsvis konstaterar uppsatsen att avregleringen haft betydande inverkan på telefonimarknaden men inte i någon större utsträckning lett till lägre priser eller ett särskillt differentierat utbud. För att öka konkurrensen på marknaden krävs att ägandet av nätet avregleras.. ...
Sektionen om lægebehandling på jw.org er udarbejdet som en informationsressource primært for læger og andre sundhedspersoner. Den giver hverken medicinsk vejledning eller anbefaling af behandling, og den erstatter ikke en velkvalificeret læge. Den lægevidenskabelige litteratur der er henvist til, er ikke udgivet af Jehovas Vidner, men den skitserer alternative strategier til transfusion som kan overvejes. Det er den enkelte læges ansvar at holde sig ajour med den nyeste information, drøfte behandlingsmetoder og hjælpe patienter til at træffe valg der harmonerer med deres lidelse, ønsker, værdier og religiøse overbevisning. Ikke alle anførte strategier er passende eller acceptable for alle patienter.. Til patienter: Søg altid råd hos din egen læge eller den behandlingsansvarlige læge vedrørende medicinske lidelser og behandling. Henvend dig til en læge hvis du har mistanke om at du er syg.. Denne hjemmeside reguleres efter dens anvendelsesvilkår.. ...
3 April 2013 , Posted in: Design, Design Lab, Övrigt, Trender i hemmet Den ledande globala designtävlingen presenterar de 100+ bästa bidragen Tre koncept från studenter på svenska designskolor har valts ut bland de 1 700 inkommande bidragen för att presenteras som några av de bästa idéerna i Electrolux Design Lab 2013. Designstudenter världen över har fått skicka in koncept på temat Urban Living inom fokusområdena Social Cooking, Effortless […]. Tags: 100+ concepts, Centro, Dawid Dawod, Design, Dessert-draw, electrolux design lab, Global Chef, Inspired Urban Living, Johan Skoog, Rongrong Zhang, Social Cooking, Thomas Johansson. ...
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