Ribonuclease A (RNAse A) is an endoribonuclease, that specifically cleaves single-stranded RNA 3 to pyrimidine residues (cytosine, uracil). Thereby, it generates pyrimidine-3-phosphate or oligonucleotides with terminal pyrimidine-3-phosphates. The pH-optimum is in the range of 7.0 to 7.5. RNAse is used for the purification of RNA-free DNA, for the removal of non-hybridised regions of RNA : DNA hybrides or as a molecular weight marker. The enzyme is inhibited by diethyl pyrocarbonate (DEPC), guanidinium salts (4M GuaSCN), ß-mercaptoethanol, heavy metals, vanadyl-ribonucleoside-complexes, RNAse- inhibitor from human placenta and competitively by DNA, respectively. Regarding the latter, the effect of denatured DNA is higher than by native nucleic acids. Nevertheless, RNAse A is very active under very different conditions and difficult to inactivate. At low salt concentrations (up to 100mM NaCl), RNAse A cleaves single- and double-stranded RNA and RNA in RNA : DNA hybrides. Under high salt ...
Abstract: While the Cu(II) binding sites of the prion protein have been well studied under Cu-saturation conditions the identity of the residues involved in coordinating Cu(II) at low stoichiometries and the order in which the binding sites load with Cu(II) remain unresolved. In this study we have used two mass spectrometry based methods to gather insight into Cu(II)-prion binding under different stoichiometric loadings of Cu(II). The first method uses metal-catalyzed oxidation reactions to site specifically modify the residues bound to Cu(II) in solution and the second method determines Cu binding sites based on the protection of His from modification by diethyl pyrocarbonate when this residue binds Cu(II) in solution. For both methods the residues that are labeled by these reactions can then be unambiguously identified using tandem mass spectrometry. Upon applying these two complementary methods to a construct of the prion protein that contains residues 23-28 and 57-98 several noteworthy ...
If you have ever worked with RNA, you know about DEPC (diethylpyrocarbonate). You add it to water at a concentration of 0.1%, shake or stir, incubate at
Learn about using DEPC, DiethylenePyrocarbonate for RNA work. Includes information on inactivating RNAses using DEPC, and how to prepare buffer solutions recipe with DEPC and even TRIS buffers recipes.
Protein is a dietary component essential for nutritional homeostasis in humans. Normally, ingested protein undergoes a complex series of degradative processes following the action of gastric, pancreatic and small intestinal enzymes. The result of this proteolytic activity is a mixture of amino acids and small peptides. Amino acids (AAs) are transported into the enterocyte (intestinal epithelial cell) by a variety of AA transporters that are specific for cationic (basic) AA, neutral AA, and anionic (acidic) AA. Small peptides are absorbed into enterocytes by the PEPT1 transporter. Inside enterocytes peptides are hydrolyzed, and the resulting amino acids are released together with those absorbed by AA transporters into blood via multiple, basolateral, AA transporters. Hydrolysis-resistant peptides, however, are transported out of the cells by a basolateral peptide transporter that has not been identified molecularly ...
Looking for diethyl sulfate? Find out information about diethyl sulfate. 2SO4 A colorless oil with a peppermint odor, and boiling at 208°C; used as an intermediate in organic synthesis. Also known as ethyl sulfate Explanation of diethyl sulfate
Traces of DEPC modify purine residues (A+G) in RNA by carboxymethylation. Therefore, DEPC must always be removed from solutions or containers by autoclaving or heating at 100°C for 15 min. Cell-free translation of carboxymethylated RNA will yield less protein than with unmodified template. Hybridisation is not seriously affected unless the RNA probe is heavily ...
diethyl 2-(3-chloropropyl)propanedioate 18719-43-2 NMR spectrum, diethyl 2-(3-chloropropyl)propanedioate H-NMR spectral analysis, diethyl 2-(3-chloropropyl)propanedioate C-NMR spectral analysis ect.
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DIETHYL BENZYLIDENEMALONATE 5292-53-5 MSDS report, DIETHYL BENZYLIDENEMALONATE MSDS safety technical specifications search, DIETHYL BENZYLIDENEMALONATE safety information specifications ect.
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Diethyl(propyl)amine | C7H17N | CID 78206 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
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The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
The interactive plot requires a browser with JavaScript and HTML 5 canvas support. Select a region with data to zoom. Select a region with no data or click the mouse on the plot to revert to the orginal display. Moving the mouse pointer over or near a line will display the coordinates of the peak. The number of digits shown do not reflect the uncertainty of the value. ...
Reaction of rabbit skeletal-muscle AMP deaminase with a low molar excess of diethyl pyrocarbonate results in conversion of the enzyme into a species with one or two carbethoxylated histidine residues per subunit that retains sensitivity to ATP at pH 7.1 but, unlike the native enzyme, it is not sensitive to regulation by ATP at pH 6.5. This effect mimics that exerted on the enzyme by limited proteolysis with trypsin, which removes the 95-residue N-terminal region from the 80 kDa enzyme subunit. These observations suggest involvement of some histidine residues localized in the region HHEMQAHILH (residues 51-60) in the regulatory mechanism which stabilizes the binding of ATP to its inhibitory site at acidic pH. Carbethoxylation of two histidine residues per subunit abolishes the inhibition by ATP of the proteolysed enzyme at pH 7.1, suggesting the obligatory participation of a second class of histidine residues, localized in the 70 kDa subunit core, in the mechanism of the pH-dependent inhibition ...
Glucosylceramide synthase (GCS) catalyses the transfer of glucose from UDP-glucose (UDP-Glc) to ceramide to form glucosylceramide, the common precursor of most higher-order glycosphingolipids. Inhibition of GCS activity has been proposed as a possible target of chemotherapeutic agents for a number of diseases, including cancer. Design of new GCS inhibitors with desirable pharmaceutical properties is hampered by lack of knowledge of the secondary structure or catalytic mechanism of the GCS protein. Thus we cloned the rat homologue of GCS to begin studies to identify its catalytic regions. The histidine-modifying agent diethyl pyrocarbonate (DEPC) inhibited recombinant rat GCS expressed in bacteria; this inhibition was rapidly reversible by hydroxylamine and could be diminished by preincubation of GCS with UDP-Glc. These data suggest that DEPC acts on histidine residues within or near the UDP-Glc-binding site of GCS. Mutant proteins were expressed in which the eight histidine residues in GCS were
Abstract: Antigenic properties of human, pig, rabbit and rat lactate dehydrogenase (LDH) M4-isozyme as well as of human LDH-C4 were studied using antipeptide antibodies against pig LDH-M4 fragment (180-214). Amino acid sequence 180-214, containing His-195, which is involved in the active site of LDH isozymes, proved to be total antigenic determinant only for human and pig M4-isozymes. Amino acid sequence of total antigenic determinant did not allow any substitutions of essential amino acid residues. His-195, participating in substrate binding, was not involved immediately in reactions with antibodies as shown by means of chemical modification of pig M4-isozyme with diethyl pyrocarbonate and after production of antibodies against the modified isoenzyme ...
TY - JOUR. T1 - Study of diethyl dithiophosphate adsorption on chalcopyrite and tennantite at varied phs. AU - Petrus, H. T.B.M.. AU - Hirajima, Tsuyoshi. AU - Sasaki, Keiko. AU - Okamotob, H.. PY - 2011/9/1. Y1 - 2011/9/1. N2 - The kinetics of diethyl dithiophosphate adsorption on chalcopyrite and tennantite has been studied by UV-visible spectroscopy at pH values of 4, 6, and 9. The concentration of diethyl dithiophosphate in the solution has been monitored as a function of time and pH for both minerals. It was found that the adsorption tendency of diethyl dithiophosphate on both minerals decreased with the increasing pH treatments. This is due to the existence of metal hydroxide species onto the mineral surface in more alkaline condition inhibiting the adsorption of diethyl dithiophosphate species. In comparison to that of chalcopyrite, tennantite possessed slightly higher adsorption of diethyl dithiophosphate in acid condition, while vice versa correlation observed at other pH treatments at ...
3.6 mL - 5M NaCl (DEPC treated). 0.6 mL - Tris 1M pH 6.5 (use DEPC water but do not mix DEPC in Tris Buffer). 3.0 mL - 0.2M NaPO4 pH 6.8(DEPC treated). 0.6mL - 0.5M EDTA (DEPC treated). 2.2 mL - DEPC water. ----. 10 mL -- Total. Pre - Hybridization Mix for 100 slides ( without formamide). 6X salts -- 3333.00 uL. Dextran Sulfate(50%)-- 4000.00 uL. 50X Denhards ------ 400.00 uL. t-RNA (100mg/mL)---- 200.00 uL. DEPC water ------ 567.00 uL. 8500.00 uL. Mix everything in the Nutator for 1-2 hrs. Then aliquate into 500 ul into an orange cap screw tubes and stored in -70 0 C freezer. Cut the tip of 1000ul because the pre- Hyb is viscous. Formamide.. Aliquate 1ml of fomamide in 1ml orange screw cap tube and store in -70 .. Final Hybridization Mix.. Mix equal quantity of pre-hybridization and formamide for hybridization. This step you can do at the time of experiment.. 50 % Dextran Sulfate ( 40ul-final Volume). Add 20 gram of Dextran Sulfate (on Sridevis cabinet) to 10ml of dd water in a 50 ml conical ...
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Malonic acid (also called Propanedioic Acid ) is a white crystalline C-3 dicarboxylic acid; melting at 135 -136 C; readily soluble in water, alcohol and ether; solution in water is medium-strong acidic. It can be derived by oxidizing malic acid or by the hydrolysis of cyanacetic acid. Malonic acid itself is rather unstable and has few applications. Its diethyl ester (diethyl malonate) is more important commercially. Diethyl malonate, a colourless, fragrant liquid boiling at 199 C, is prepared by the reaction of monochloroacetatic acid with methanol, carbon monoxide or by the reaction cyanoacetic acid (the half nitriled-malonic acid) with ethyl alcohol. Malonic acid and its esters contain active methylene groups which have relatively acidic alpha-protons due to H atoms adjacent to two carbonyl groups. The reactivity of its methylene group provide the sequence of reactions of alkylation, hydrolysis of the esters and decarboxylation resulting in substituted ketones. The methylene groups in ...
The voltage-sensitive phosphoinositide phosphatases provide a mechanism to couple changes in the transmembrane electrical potential to intracellular signal transduction pathways. These proteins share a domain architecture that is conserved in deuterostomes. However, gene duplication events in primates, including humans, give rise to the paralogs TPTE and TPTE2 that retain protein domain organization but, in the case of TPTE, have lost catalytic activity. Here, we present evidence that these human proteins contain a functional voltage sensor, similar to that in nonmammalian orthologs. However, domains of these human proteins can also generate a noninactivating outward current that is not observed in zebra fish or tunicate orthologs. This outward current has the anticipated characteristics of a voltage-sensitive proton current and is due to the appearance of a single histidine residue in the S4 transmembrane segment of the voltage sensor. Histidine is observed at this position only during the ...
A strategy for rational enzyme design is reported and illustrated by the engineering of a protein catalyst for thiol-ester hydrolysis. Five mutants of human glutathione (GSH; gamma-Glu-Cys-Gly) transferase A1-1 were designed in the search for a catalyst and to provide a set of proteins from which the reaction mechanism could be elucidated. The single mutant A216H catalyzed the hydrolysis of the S-benzoyl ester of GSH under turnover conditions with a k(cat)/K(M) of 156 M(-1) x min(-1), and a catalytic proficiency of ,10(7) M(-1) when compared with the first-order rate constant of the uncatalyzed reaction. The wild-type enzyme did not hydrolyze the substrate, and thus, the introduction of a single histidine residue transformed the wild-type enzyme into a turnover system for thiol-ester hydrolysis. By kinetic analysis of single, double, and triple mutants, as well as from studies of reaction products, it was established that the enzyme A216H catalyzes the hydrolysis of the thiol-ester substrate by ...
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This page contains information on the chemical Phosphoric acid, diethyl (1-piperidino-2-propyl) ester including: 5 synonyms/identifiers.
see article for more examples. Abstract. Diethyl N-Boc-iminomalonate, prepared on multi-gram scale, served as a stable and highly reactive electrophilic glycine equivalent which reacted with organomagnesium compounds affording substituted aryl N-Boc-aminomalonates. Subsequent hydrolysis produced arylglycines.. ...
Diethyl 1-cyanoethyl phosphate | C7H14NO4P | CID 99791 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
68910-04-3 - VESIIXDFIZNBOJ-UHFFFAOYSA-N - Thiodicarbonic acid ((HO)C(O)SC(S)(OH)), diethyl ester, reaction products with 3-methyl-1,2-benzenediamine and o-toluidine - Similar structures search, synonyms, formulas, resource links, and other chemical information.
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The Drosophila ventral nerve cord (VNC) receives and processes descending signals from the brain to produce a variety of coordinated locomotor outputs. It also integrates sensory information from the periphery and sends ascending signals to the brain. We used single-cell transcriptomics to generate an unbiased classification of cellular diversity in the VNC of five-day old adult flies. We produced an atlas of 26,000 high-quality cells, representing more than 100 transcriptionally distinct cell types. The predominant gene signatures defining neuronal cell types reflect shared developmental histories based on the neuroblast from which cells were derived, as well as their birth order. The relative position of cells along the anterior-posterior axis could also be assigned using adult Hox gene expression. This single-cell transcriptional atlas of the adult fly VNC will be a valuable resource for future studies of neurodevelopment and behavior.
3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored by incubation with hydroxylamine. Protection against DEPC inactivation is afforded by a substrate analogue, suggesting an ac
Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS). Mediates the sodium-independent uptake of p- aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3-azido- 3-deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro- phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2- furanpropionate (CMPF), cidofovir, adefovir, 9-(2- phosphonylmethoxyethyl) guanine (PMEG), 9-(2- phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p- chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid. PAH uptake is inhibited by ...
We have shown previously (Schwartz, M. A., and E. J. Luna. 1986. J. Cell Biol. 102: 2067-2075) that actin binds with positive cooperativity to plasma membranes from Dictyostelium discoideum. Actin is polymerized at the membrane surface even at concentrations well below the critical concentration for polymerization in solution. Low salt buffer that blocks actin polymerization in solution also prevents actin binding to membranes. To further explore the relationship between actin polymerization and binding to membranes, we prepared four chemically modified actins that appear to be incapable of polymerizing in solution. Three of these derivatives also lost their ability to bind to membranes. The fourth derivative (EF actin), in which histidine-40 is labeled with ethoxyformic anhydride, binds to membranes with reduced affinity. Binding curves exhibit positive cooperativity, and cross-linking experiments show that membrane-bound actin is multimeric. Thus, binding and polymerization are tightly ...
L-Threonine dehydrogenase (TDH) from Escherichia coli is rapidly inactivated and develops a new absorbance peak at 347 nm when incubated with N-ethyl-5-phenylisoxazolium-3-sulfonate (Woodwards reagent K, WRK). The cofactors, NAD+ or NADH (1.5 mM), provide complete protection against inactivation; L-threonine (60 mM) is approximately 50% as effective. Tryptic digestion of WRK-modified TDH followed by HPLC fractionation (pH 6.2) yields four 340-nm-absorbing peptides, two of which are absent from enzyme incubated with WRK and NAD+. Peptide I has the sequence TAICGTDVH (TDH residues 35-43), whereas peptide II is TAICGTDVHIY (residues 35-45). Peptides not protected are TMLDTMNHGGR (III, residues 248-258) and NCRGGRTHLCR (IV, residues 98-108). Absorbance spectra of these WRK-peptides were compared with WRK adducts of imidazole, 2-hydroxyethanethiolate, and acetate. Peptides III and IV have pH-dependent lambda max values (340-350 nm), consistent with histidine modification. Peptide I has ...
TY - JOUR. T1 - Spectroscopic and electrochemical studies of two distal diethyl ester azocalix[4]arene derivatives. AU - Dong, Yunyan. AU - Kim, Tae Hyun. AU - Kim, Hyun Jung. AU - Lee, Min Hee. AU - Lee, Su Yeon. AU - Mahajan, Rakesh Kumar. AU - Kim, Hasuck. AU - Kim, Jong Seung. PY - 2009/4/1. Y1 - 2009/4/1. N2 - Binding properties of two distal diethyl ester azocalix[4]arene derivatives (1-3) with alkali, alkaline earth and transition metal ions were investigated by UV/vis spectroscopy and voltammetry. With respect to the chromogenic and voltammetric behavior of hosts upon metal ion complexation, two distal diethyl ester azocalix[4]arene derivatives (1 and 2) showed a good selectivity toward Ca2+ over many cations. The presence of two proximal hydroxyl groups and two esters in ligands was observed to play an important role in exhibiting cation complexation by extensive control experiments with corresponding azocalix[4]arene derivatives.. AB - Binding properties of two distal diethyl ester ...
Looking for diethyl carbonate? Find out information about diethyl carbonate. 2CO3 Stable, colorless liquid with mild aroma, boiling at 126°C; soluble with most organic solvents; used as a solvent and for chemical synthesis. Explanation of diethyl carbonate
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This page contains information on the chemical Ammonium, diethyl(2-hydroxyethyl)methyl-, iodide, 2-phenylvalerate including: 4 synonyms/identifiers.
Sigma-Aldrich offers Aldrich-774278, Diethyl sulfite for your research needs. Find product specific information including CAS, MSDS, protocols and references.
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Dortmund Data bank - Thermophysical Properties | Diethyl ether, CAS Number: 60-29-7 | Methanol, CAS Number: 67-56-1 | SpringerMaterials 2014
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Citation: Bishop, JB, Morris, RW, Seely, JC, Hughes, LA, Cain, KT, Generoso, WM. Alterations in the Reproductive Patterns of Female Mice Exposed to Xenobiotics. Fund. and Appl. Tox. 40: 191-204 (1997 ...
Phosphoryl transfer between the PTS components is thought to proceed by nucleophilic attack of the active histidine residue on the phospho-histidine of the target protein ...
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ஹிஸ்டிடின் (Histidine) [குறுக்கம்: His (அ) H][2] என்னும் அமினோ அமிலம் ஒரு அத்தியாவசிய அமினோ அமிலமாகும். இதனுடைய வாய்பாடு: C6H9N3O2. இது விலங்குகளினால்/மனிதர்களால் தயாரிக்கப்படுவதில்லை. எனவே, நாம் உண்ணும் புரதங்களிலிருந்துப் பெறப்படுகிறது. இதன் குறிமுறையன்கள்: CAU மற்றும் CAC. ஹிஸ்டிடின், நேர்மின்மம் கொண்ட இமிடசோல் தொகுதியை வினைசார் தொகுதியாகக்கொண்டுள்ளது. ...
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