This study was performed in the opossum lower esophageal sphincter (LES) smooth muscle strips to determine the action of the heme oxygenase inhibitor zinc protoporphyrin IX (ZnPP IX) on the relaxant effect of vasoactive intestinal polypeptide and isoproterenol, which are known to stimulate adenylate cyclase (AC) via G protein coupling, and of the direct activator of AC catalytic subunit forskolin. To investigate the cGMP pathway, we examined the effect of atrial natriuretic factor known to activate the receptor linked to the particulate guanylate cyclase via G protein coupling and that of sodium nitroprusside [nitric oxide (NO) donor], authentic NO and carbon monoxide, which stimulate the intracellular soluble fraction of GC. The smooth muscle relaxation caused by nonadrenergic noncholinergic (NANC) nerve stimulation also was investigated. ZnPP IX caused concentration-dependent attenuation of the relaxant effect of vasoactive intestinal polypeptide, isoproterenol and atrial natriuretic factor ...

Drugs that bound to the peripheral-type or mitochondrial benzodiazepine receptors in rat kidney mitochondria produced several effects on mitochondrial respiration with succinate and malate/pyruvate as substrates. These drugs increased state IV and decreased state III respiration rates, which resulted in a significant decrease in the respiratory control ratio. ADP: O ratios were not affected. The receptor binding affinities of a set of 10 compounds (Ro5-4864, PK11195, diazepam, mesoporphyrin IX, flunitrazepam, deuteroporphyrin IX, dipyridamole, dibutylphthalate, cyclosporin A, and CL259,763) correlated over a concentration range of almost 4 orders of magnitude with their potencies at inhibiting respiratory control (r = 0.95). The anxiolytic benzodiazepine clonazepam had no effect on mitochondrial respiratory control and bound with negligible affinity to the receptor. The magnitude of the effect of Ro5-4865 on respiration increased in parallel with the density of mitochondrial benzodiazepine ...

To study the role of endogenous carbon monoxide (CO) in collagen metabolism during hypoxic pulmonary vascular remodeling, a total of 18 Wistar rats were used in the study and they were randomly divided into three groups: hypoxia group (n=6), hypoxia with zinc protoporphyrin-IX (ZnPP-IX) group (n=6) and control group (n=6). The measurement of mean pulmonary artery pressure (mPAP) and carboxyhemoglobin (HbCO) formation in lung tissue homogenates was measured. A morphometric analysis of pulmonary vessels was performed, in which the percentage of muscularized arteries (MA); partially muscularized arteries (PMA) and nonmuscularized arteries (NMV) in small and median pulmonary vessels, relative medial thickness (RMT) and relative medial area (RMA) of pulmonary arteries were analyzed. Collagen type I and III and transforming growth factor-beta(3) (TGF-beta(3)) expressions were detected by immunohistochemical assay. The expressions of procollagen type I and III and TGF-beta(3) mRNA were detected by in ...

Selective inhibition of heme oxygenase is an important strategy in design of potent inhibitors of enzyme for the treatment of neonatal jaundice, cancer and many more. QSAR analysis is employed for a given set of compounds containing imidazole pharmacophore in order to establish a relationship between the biological activity and related descriptors, which provides us an idea to gain a potent inhibitor with lesser side effects. In this paper we present results of 2D and 3D QSAR studies of series of 26 molecules containing imidazole pharmacophore as selective heme oxygenase inhibitor using V Life MDS 3.5 Software. The 2D QSAR studies was performed using Partial Least Square Regression method and the 3D QSAR studies was performed using k- Nearest Neighbor Molecular Field Analysis(kNN- MFA) method. The analysis has produced good predictive and statistically significant QSAR models. 2D QSAR studies produced good statistical model with r2 value 0.8487, cross validated r2 value 0.6553 and prd_r2 value ...

Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced liver injury. However, its role on liver fibrosis remains unclear. This study aims to elucidate the effect and the mechanism of HO-1 in nutritional fibrosing steatohepatitis in mice. Male C57BL/6J mice were fed with a methionine-choline deficient (MCD) diet for eight weeks to induce hepatic fibrosis. HO-1 chemical inducer (hemin), HO-1 chemical inhibitor zinc protoporphyrin IX (ZnPP-IX) and/or adenovirus carrying HO-1 gene (Ad-HO-1) were administered to mice, respectively. Liver injury was assessed by serum ALT, AST levels and histological examination; hepatic lipid peroxides levels were determined; the expression levels of several fibrogenic related genes were assayed by real-time quantitative PCR and Western blot. MCD feeding mice showed progressive hepatic injury including hepatic steatosis, inflammatory infiltration and fibrosis. Induction of HO-1 by hemin or Ad-HO-1 significantly attenuated the

Vítek, Libor; Gbelcová, Helena; Muchová, Lucie; Váňová, Kateřina; Zelenka, Jaroslav; Koníčková, Renata; Šuk, Jakub; Zadinova, Marie; Knejzlík, Zdeněk; Ahmad, Shakil; Fujisawa, Takeshi; Ahmed, Asif and Ruml, Tomáš (2014). Antiproliferative effects of carbon monoxide on pancreatic cancer. Digestive and liver disease, 46 (4), pp. 369-375. Vítek, Libor; Gbelcová, Helena; Muchová, Lucie; Váňová, Kateřina; Zelenka, Jaroslav; Koníčková, Renata; Šuk, Jakub; Zadinova, Marie; Knejzlík, Zdeněk; Ahmad, Shakil; Fujisawa, Takeshi; Ahmed, Asif and Ruml, Tomáš (2014). Antiproliferative effects of carbon monoxide on pancreatic cancer. Digestive and Liver Disease, 46 (4), pp. 369-375. Nayeri, Unzila A.; Buhimschi, Irina A.; Laky, Christine A.; Cross, Sarah N.; Duzyj, Christina M.; Ramma, Wenda; Sibai, Baha M.; Funai, Edmund F.; Ahmed, Asif and Buhimschi, Catalin S. (2014). Antenatal corticosteroids impact the inflammatory rather than the antiangiogenic profile of women with ...

4BIO: 8-Hydroxyquinoline-5-Carboxylic Acid is a Broad Spectrum 2-Oxoglutarate Oxygenase Inhibitor which Causes Iron Translocation

Porphyrin-cn web supplied various porphyrin卟啉催化剂, containing fluorescence porphyrin porphine 917-23-7 & Fe porphyrin Mn porphyrin Cu porphyrin Co porphyrin Ni porphyrin Zn porphyrin & metalloporphyrin

The preparation of phosphorescent metalloporphyrin labelling reagents and their use for preparation of phosphorescent conjugates with biomolecules. The labelling reagents obtainable are water soluble monofunctional derivatives of Pt- and Pd-coproporphyrins, where the term

When an orthodontic appliance is attached to the lingual side (back side) of teeth which is invisible from the outside, a large difference in level of the surface between adjacent teeth requires utilization of a wire bent in a complicated fashion. It has been found, however, that the difference in level between adjacent teeth is very small in a horizontal plane at a boundary between a crown and a root, thereby making it possible to draw a smooth envelope therealong. Based on this finding, with a view to placing a wire along such an envelope, a

Since ZnDPBG and ZnPP inhibit heme oxygenase-mediated conversion of heme to carbon monoxide and biliverdin,9 10 11 12 the effects of these inhibitors on hemodynamics may provide insights into the physiological influence of heme oxygenase products on BP regulation. ZnDPBG and ZnPP administration produces pressor responses that are maximal within 5 minutes and maintained for approximately 1 hour. ZnDPBG-induced increases in arterial pressure are paralleled by increases in total peripheral resistance, establishing systemic vasoconstriction as the major hemodynamic determinant of the increase in BP. Importantly, nonmetallic DPBG, which does not inhibit heme oxygenase activity, does not affect hemodynamics. This strengthens the argument that the pressor effect of ZnDPBG is a consequence of decreased heme oxygenase activity. If so, these findings imply that heme oxygenase activity is subserving a vasodepressor function by promoting vasodilation.. Elevation of BP after pretreatment with an inhibitor of ...

TY - JOUR. T1 - Study by Fourier Transform Infrared Spectroscopy of the Adsorption of Carbon Monoxide on a Nickel Electrode at pH 3-14. AU - Cuesta, Angel. AU - Gutierrez, Claudio. PY - 1998. Y1 - 1998. M3 - Article. VL - 14. SP - 3397. EP - 3404. JO - Langmuir. JF - Langmuir. SN - 0743-7463. ER - ...

This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...

Since carbon monoxide is formed by the incomplete combustion of a carbon-containing material, carbon monoxide is a hazard at any location where these materials are present. Common sources of carbon monoxide on construction sites include:. ...

Read the latest Meridian stories, Warnings over dangers of carbon monoxide on ITV News, videos, stories and all the latest Meridian news

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society copyright © American Chemical Society after peer review and technical editing by the publisher.To access the final edited and published work see http://dx.doi.org/10.1021/ja8076389 ...

Engangshansker av nitril er 3 ganger sterkere enn vinyl eller latex engangs hansker og mer komfortabel, samt gir bedre beskyttelse.. • Mindre klamme hender sammenliknet med vinyl- eller latexhansker.. • Pakket 100 hansker i dispenser eske.. • Tykkelse 0.14mm +- 0.01mm.. ...

Photochemistry and Photobiology publishes on photoscience, like primary interaction of light with molecules, cells, and tissue to biological responses.

TY - JOUR. T1 - Steady-state kinetics of the catalytic reduction of nitrogen dioxide by carbon monoxide on platinum. AU - Wickham, D. T.. AU - Koel, B. E.. PY - 1988/12. Y1 - 1988/12. N2 - The steady-state kinetics of the reduction of nitrogen dioxide (NO2) with carbon monoxide (CO) on a polycrystalline platinum foil have been investigated using reactant pressures between 1 × 10-7 and 5 × 10-6 Torr. At temperatures less than 400 K, the primary products formed are carbon dioxide (CO2) and nitric oxide (NO). The CO2 formation rate at temperatures less than 400 K is at least an order of magnitude greater for this reaction than for those of either CO + O2 or CO + NO under similar reaction conditions. The reaction rate is first-order in CO pressure when PCO PNO2 , 0.25 and first-order in NO2 pressure when PCO PNO2 , 1. The activation energy when PCO PNO2 = 0.10 is 7.2 kcal mole-1 which is consistent with the activation energy for CO + O2 under similar conditions. However, when PCO PNO2 = 1.5, the ...

TY - JOUR. T1 - Influences of carbon monoxide on the binding of oxygen, carbon dioxide, proton and 2,3-diphosphoglycerate to human hemoglobin.. AU - Yamaguchi, K.. AU - Mori, M.. AU - Kawai, A.. AU - Yokoyama, T.. PY - 1988. Y1 - 1988. N2 - In an attempt to estimate the influences of CO on the CO2 Bohr effect and the 2,3-diphosphoglycerate (DPG) effect linked to the reversible binding of O2 to the hemoglobin molecule (Hb), O2 dissociation curves of human blood in the presence of CO were investigated at 37 degrees C over a DPG concentration ranging from 2.2 to 4.3 mmol/(1RBC) and a pH range of 7.2 to 7.6. The sample with a low DPG concentration was made by incubating whole blood for 6 hrs, whereas the saturation of Hb with CO, SCO in the sample was adjusted by anaerobically mixing completely carboxygenated blood with that free of O2 and CO so as to give the final SCO at either 0, 10, 15, 20, 40 or 50%. The blood samples thus prepared were diluted at 1:100 in isotonic buffer solution and were ...

TY - JOUR. T1 - Cerebral vascular endothelial heme oxygenase. T2 - Expression, localization, and activation by glutamate. AU - Parfenova, Helena. AU - Neff, Robert A.. AU - Alonso, Julie S.. AU - Shlopov, Boris V.. AU - Jamal, Chaklader N.. AU - Sarkisova, Svetlana A.. AU - Leffler, Charles W.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2001. Y1 - 2001. N2 - Endogenous carbon monoxide (CO) contributes to vasodilator responses of cerebral microvessels in newborn pigs. We investigated the expression, intracellular localization, and activity of heme oxygenase (HO), the key enzyme in CO production, in quiescent cerebral microvascular endothelial cells (CMVEC) from newborn pigs. HO-1 and HO-2 isoforms were detected by RT-PCR, immunoblotting, and immunofluorescence. HO-1 and HO-2 are membrane-bound proteins that have a strong preference for the nuclear envelope and perinuclear area of the cytoplasm. Betamethasone (10-6 to 10-4 M for 48 h) was associated with upregulation ...

The equilibrium binding of hydroxyethyl vinyl deuteroporphyrin (HVD) and of irreversible porphyrin aggregates to human serum albumin was studied at the molecular level. This protein may function as an endogenous drug carrier for porphyrins in photodynamic therapy of tumours. HVD-protein binding studies revealed two types of binding sites, which are attributed to the two HVD isomers. The binding constant for the high-affinity isomer, 2.1 (+/- 0.3) x 10(8) M-1, is similar to that previously determined for protoporphyrin. At the same time the binding constant for the lower-affinity HVD isomer, 1.8(+/- 0.3) x 10(6) M-1, is similar to that previously determined for haematoporphyrin. Irreversible porphyrin aggregates were purified from the haematoporphyrin derivative and from Photofrin and are defined by spectral and chromatographic data. Gel-exclusion studies indicate that the dominant size of these aggregates is ten porphyrin monomeric units. The protein-binding constant of these aggregates is ...

2015 Haugaa et al. licensee BioMed Central. Introduction: Tissue reperfusion following hemorrhagic shock may paradoxically cause tissue injury and organ dysfunction by mitochondrial free radical expression. Both nitrite and carbon monoxide (CO) may protect from this reperfusion injury by limiting mitochondrial free radial production. We explored the effects of very small doses of inhaled nitrite and CO on tissue injury in a porcine model of hemorrhagic shock. Methods: Twenty pigs (mean wt. 30.6kg, range 27.2 to 36.4kg) had microdialysis catheters inserted in muscle, peritoneum, and liver to measure lactate, pyruvate, glucose, glycerol, and nitrite. Nineteen of the pigs were bled at a rate of 20ml/min to a mean arterial pressure of 30mmHg and kept between 30 and 40mmHg for 90minutes and then resuscitated. One pig was instrumented but not bled (sham). Hemorrhaged animals were randomized to inhale nothing (control, n=7), 11mg nitrite (nitrite, n=7) or 250ppm CO (CO, n=5) over 30minutes before fluid ...

The present invention relates to metallized porphyrin derivatives having the formula: ##STR1## wherein the R and M variables are as defined in the specification. Said porphyrin derivatives having uses as antitumor agents, diagnostics agents, or in therapy.

GenBank) ChlI component of cobalt chelatase involved in B12 biosynthesis or ChlD component of cobalt chelatase involved in B12 ...

Background. Studies in vitro, in animal models, and in adult and newborn humans have demonstrated that certain tin(Sn)-porphyrins that competitively inhibit the activity of heme oxygenase, the rate-limiting enzyme in heme catabolism, reduce production of bilirubin and can thereby substantially diminish plasma levels of the bile pigment.. Objectives. To assess the effectiveness of increasing doses of the heme oxygenase inhibitor, Sn-mesoporphyrin (SnMP), in moderating the development of significant hyperbilirubinemia and thus the requirements for phototherapy in preterm newborns.. Methods. In five randomized, blinded, placebo-controlled trials, SnMP in increasing doses from 1 µmol to 6 µmol/kg body weight was administered intramuscularly in the first 24 hours of life in preterm newborns of 210 to 251 days gestational age. Special blue lamps (Phillips F20T12/BB) were used for phototherapy in newborns exceeding a predetermined plasma bilirubin concentration, irrespective of study ...

TY - JOUR. T1 - Colon and pancreas tumors enhance coagulation. T2 - Role of hemeoxygenase-1. AU - Nielsen, Vance G.. AU - Nfonsam, Valentine N.. AU - Matika, Ryan W.. AU - Ong, Evan S.. AU - Jie, Tun. AU - Warneke, James A.. AU - Steinbrenner, Evangelina B.. PY - 2014/7. Y1 - 2014/7. N2 - Colon and pancreatic cancer are associated with significant thrombophilia. Colon and pancreas tumor cells have an increase in hemeoxygenase-1 (HO-1) activity, the endogenous enzyme responsible for carbon monoxide production. Given that carbon monoxide enhances plasmatic coagulation, we determined if patients undergoing resection of colon and pancreatic tumors had an increase in endogenous carbon monoxide and plasmatic hypercoagulability. Patients with colon (n = 17) and pancreatic (n = 10) tumors were studied. Carbon monoxide was determined by the measurement of carboxyhemoglobin (COHb). A thrombelastographic method to assess plasma coagulation kinetics and formation of carboxyhemefibrinogen (COHF) was ...

article{9adb9228-327d-4897-80c5-5e3c43e03fe1, abstract = {During biosynthesis of chlorophyll, Mg2+ is inserted into protoporphyrin IX by magnesium chelatase. This enzyme consists of three different subunits of approximate to 40, 70 and 140 kDa. Seven barley mutants deficient in the 40 kDa magnesium chelatase subunit were analysed and it was found that this subunit is essential for the maintenance of the 70 kDa subunit, but not the 140 kDa subunit. The 40 kDa subunit has been shown to belong to the family of proteins called ATPases associated with various cellular activities, known to form ring-shaped oligomeric complexes working as molecular chaperones. Three of the seven barley mutants are semidominant mis-sense mutations leading to changes of conserved amino acid residues in the 40 kDa protein. Using the Rhodobacter capsulatus 40 and 70 kDa magnesium chelatase subunits we have analysed the effect of these mutations. Although having no ATPase activity, the deficient 40 kDa subunit could still ...

Poster (2017, October). Background: Infections due to the zoophilic dermatophyte Trichophyton benhamiae are being more frequently diagnosed in Belgium since a few years. This species has been recently included in the T ... [more ▼]. Background: Infections due to the zoophilic dermatophyte Trichophyton benhamiae are being more frequently diagnosed in Belgium since a few years. This species has been recently included in the T. benhamiae series together with T. concentricum, T. verrucosum and T. erinacei (De Hoog et al 2016). The strains appear in culture with a bright yellow thallus and do not easily sporulate making them difficult to identify by microscopy. Maldi-Tof MS may present an alternative to microscopic or molecular identification of this species. Molecular methods as ITS sequencing, Rep-PCR by Diversilab® system and realtime PCR by DermaGenius® kit, have been evaluated as identification methods. Methods: A total of 37 strains have been analyzed, all collected by the National ...

Porphyrin-cn web supplied various porphyrin卟啉催化剂, containing fluorescence porphyrin porphine 917-23-7 & Fe porphyrin Mn porphyrin Cu porphyrin Co porphyrin Ni porphyrin Zn porphyrin & metalloporphyrin

Next-generation photodynamic therapy agents will minimize extraneous phototoxicity by being active only at the target site. To this end, we have developed a model system to systematically investigate the excited-state quenching ability of a number of metalloporphyrins. Central metal ions that prefer …

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Introduction. At wastewater treatment plants (WWTPs), the addition of various external carbon sources to serve as energy source for the denitrifying heterotrophic bacteria, such as ethanol, methanol and acetic acid (AcOH), is used for extended nitrogen removal (Henze et al., 2002). The main advantages of external carbon sources are low sludge production and on-demand accessibility. Despite these advantages, full-scale applications of sludge hydrolysis for the internal production of volatile fatty acids (VFAs), functioning as an alternative carbon and energy source, is of interest, and has been implemented at several WWTPs (Andreasen et al., 1997; Canziani et al., 1995; Johansson et al., 1996; Jonsson et al., 1996; Ucisik et al., 2008). In wastewater terminology, sludge hydrolysis comprises all mechanisms that make slowly biodegradable substrates available for bacterial growth (Gujer et al., 1999).. The production of endogenous carbon sources by the application of sludge hydrolysis is motivated ...

Metalloporphyrin research has been firmly established as useful for mimicking certain enzymes present in living systems, and much research has gone into using these compounds to oxidise organic substrates. However, one major drawback is that there is no clear picture of the electronic factors that influence catalyst efficiency; another is that these catalysts undergo degradation that causes a subsequent loss of activity. After reviewing the literature, it became apparent that much published research focuses on epoxidation by a small and eclectic range of tetra-substituted metalloporphyrin catalysts whose main criterion for selection is that they are readily available. This thesis seeks to remedy that by looking in detail at more finely-tuned structural and electronic variations of the substituents to iron porphyrins. In order to investigate the effects of catalyst structure on epoxidation efficiency and catalysts stability a large number of iron porphyrins was synthesised. In general a ...

The effectiveness of microwave-assisted synthesis of 5,10,15,20-tetraphenylporphyrin and its corresponding to metal complexes of (Bi (III), Zn (II) and Ni (II)) are described. Moreover, the synthesized metalloporphyrins that were obtained with reasonable yields were characterized using UV-vis, 1H-NMR and IR.

Explains how the zinc protoporphyrin (ZPP) test is used, when a ZPP test is requested, and what the results of a ZPP test might mean

You are viewing an interactive 3D depiction of the molecule 3-[5-[(e)-[(5e)-5-[[5-[(e)-[4-(2-carboxyethyl)-3-methyl-5-oxo-pyrrol-2-ylidene]methyl]-3-methyl-4-vinyl-1h-pyrrol-2-yl]methylene]-4-methyl-3-vinyl-pyrrol-2-ylidene]methyl]-4-methyl-2-oxo-pyrrol-3-yl]propanoic acid (C33H26N4O6) from the PQR.

Photoinduced biological processes are complex and can often be reduced to a series of sequential events after the absorption of the photon. Each of these steps, if separable, can be compared with the evolution of a much simpler system, mimicking its essential characteristics, a biomimetic system. This stems from the very local properties of the initial event where a small reaction centre has been excited, spatially limited within the biomolecule. Gas phase conditions provide the unique way to study these model systems.

TY - JOUR. T1 - Effect of magnesium on preferential oxidation of carbon monoxide on platinum catalyst in hydrogen-rich stream. AU - Cho, Sung Ho. AU - Park, Jong Soo. AU - Choi, Seung Hoon. AU - Kim, Sung Hyun. PY - 2006/6/1. Y1 - 2006/6/1. N2 - The effects of magnesium on platinum catalyst used for the preferential oxidation of carbon monoxide for polymer electrolyte membrane fuel cell applications are investigated. The CO conversion and selectivity on Pt-Mg/Al2O3 for a H2-rich stream are 93.1 and 62.0%, respectively, but only 70.2 and 46.89% on Pt/Al2O3. The superior activity of Pt-Mg/Al2O3 for the preferential oxidation of CO is due to an increase in the hydroxyl groups that results from an increase in basicity with the addition of Mg, as well as to an increase in the electron density on the surface of the Pt catalyst. Moreover, the content of hydroxyl groups on the Pt catalysts is promoted by water vapour.. AB - The effects of magnesium on platinum catalyst used for the preferential ...

TY - JOUR. T1 - ATPase activity associated with the magnesium chelatase H-subunit of the chlorophyll biosynthetic pathway is an artefact. AU - Sirijovski, Nick. AU - Olsson, Ulf. AU - Lundqvist, Joakim. AU - Al-Karadaghi, Salam. AU - Willows, Robert D.. AU - Hansson, Mats. PY - 2006/12/15. Y1 - 2006/12/15. N2 - Magnesium chelatase inserts Mg2+ into protoporphyrin IX and is the first unique enzyme of the chlorophyll biosynthetic pathway. It is a heterotrimeric enzyme, composed of I- (40 kDa), D- (70 kDa) and H- (140 kDa) subunits. The I- and D-proteins belong to the family of AAA+ (ATPases associated with various cellular activities), but only I-subunit hydrolyses ATP to ADP. The D-subunits provide a platform for the assembly of the I-subunits, which results in a two-tiered hexameric ring complex. However, the D-subunits are unstable in the chloroplast unless ATPase active I-subunits are present. The H-subunit binds protoporphyrin and is suggested to be the catalytic subunit. Previous studies ...

Activation of heme oxygenase-1 (HO-1) has been proved to reduce damages to the liver in ischemia reperfusion injury. The objective of present study was to determine whether clinic relevant doses of isoflurane treatment could be sufficient to activate HO-1 inducing, which confers protective effect against hepatic ischemia-reperfusion injury. The hepatic artery and portal vein to the left and the median liver lobes of forty male Sprague-Dawley rats were occluded for 60 minutes. Reperfusion was allowed for 4 hours before the animal subjects were sacrificed. Six groups (n = 12) were included in the study. A negative control group received sham operation and positive control group a standard ischemia-reperfusion regimen. The third group was pretreated with isoflurane prior to the ischemia-reperfusion. The fourth group received an HO-1 inhibitor zinc protoporphyrin (Znpp) prior to the isoflurane pretreatment and the ischemia-reperfusion. The fifth group received Znpp alone before ischemia-reperfusion

The sensor is similar to other carbon nanotube devices that Swagers lab has developed in recent years, including one that detects the ripeness of fruit. All of these devices work on the same principle: Carbon nanotubes can be chemically modified so that their ability to carry an electric current changes in the presence of a particular gas.. In this case, the researchers modified the carbon nanotubes with metal-containing compounds called metalloporphyrins, which contain a central metal atom bound to several nitrogen-containing rings. Hemoglobin, which carries oxygen in the blood, is a metalloporphyrin with iron as the central atom.. For this sensor, the researchers used a metalloporphyrin with cobalt at its center. Metalloporphyrins are very good at binding to nitrogen-containing compounds called amines. Of particular interest to the researchers were the so-called biogenic amines, such as putrescine and cadaverine, which are produced by decaying meat.. When the cobalt-containing porphyrin binds ...

TY - JOUR. T1 - Synthetic Porphyrins/Metalloporphyrins Which Mimic States in Catalytic Cycle of Cytochrome P-450 and Peroxidases. AU - DAEID, N N AU - ATKINSON, S T AU - NOLAN, K B PY - 1993/7. Y1 - 1993/7. N2 - The reaction of thiolates, RS-, having electron withdrawing groups with iron(III) tetra-arylporphyrins in DMSO,in air gives low-spin Fe(III)-P(-SR)2, having hyperporphyrin spectra similar to thiolate derivatives of cytochrome P-450. Removal of air gives high spin Fe(II)-P(-SR) which on addition of CO results in H+ uptake and formation of Fe(II)-P(SHR)CO. Addition of thiols and base to the iron(III) porphyrins in toluene in the absence of air gives 4-coordinate Fe(II)-P which in the presence of CO gives Fe(II)-P(SHR)CO. Metal complexes of one of the porphyrins studied i.e. a tetraphenolic porphyrin undergo 2e- oxidation in air to give quinonoid species which oxidise peroxidase substrates. Rate constants for some of these reactions are reported.. AB - The reaction of thiolates, RS-, having ...

Zinc protoporphyrin (ZnPP) continues to be found to have got anticancer activity both and and [2C5] which is generally believed that ZnPPs anticancer activity is related to HO-1 inhibition. help elucidate the mobile systems of ZnPPs anticancer actions, but provide brand-new cancer therapeutic approaches for concentrating on the -catenin MLN0128 Wnt signaling pathway. In todays study, weve explored the mobile systems of ZnPP-induced suppression of -catenin appearance in human cancer tumor cells. The speedy and dramatic character from the ZnPP-induced suppression of -catenin proteins appearance strongly shows that this suppression is normally primarily because of -catenin proteins degradation. -catenin proteins amounts are well managed with the -catenin devastation complex thats tightly coupled towards the ubiquitin-proteasome program [9]. Hence, it is MLN0128 likely the ubiquitin-proteasome program mediates ZnPP-induced -catenin proteins degradation. However, additional proteins degradation ...

Isolated rat-liver mitochondria were osmotically lysed by suspension and washing 3 times in cold, distilled water. Pellets obtained by centrifugation at 105,000 g for 30 min were resuspended, fixed with glutaraldehyde and OsO4, and embedded in Epon 812. Thin sections show the presence of two distinct membranous populations, each of which is relatively homogeneous in size and appearance. Swollen mitochondria (∼1.5 µ in diameter), which have been stripped of their outer membranes, are largely devoid of matrix and normal matrix granules and are referred to as ghosts. The smaller (0.2 to 0.4 µ in diameter), empty appearing, vesicular elements, derived primarily from the outer mitochondrial membrane, can be differentiated from the ghosts on the basis of their smaller size and complete absence of internal structures, especially cristae. Each membranous element is enclosed by a single, continuous membrane; the double membrane organization typical of intact mitochondria is not observed. These ...

Description of disease Porphyrins - blood. Treatment Porphyrins - blood. Symptoms and causes Porphyrins - blood Prophylaxis Porphyrins - blood

Low concentrations of endogenous carbon monoxide (CO), generated primarily through degradation of heme from hemeproteins, have been shown to maintain physiological function of organs and to exert cytoprotective effects. However, high concentrations of carboxyhemoglobin (COHb), formed by CO binding to hemoglobin, potentially prevent adequate O-2 delivery to tissues by lowering arterial O-2 content. Elevated heme-protein concentrations, as found in marine mammals, are likely associated with greater heme degradation, more endogenous CO production and, consequently, elevated COHb concentrations. Therefore, we measured COHb in elephant seals, a species with large blood volumes and elevated hemoglobin and myoglobin concentrations. The levels of COHb were positively related to the total hemoglobin concentration. The maximum COHb value was 10.4% of total hemoglobin concentration. The mean (+/- s.e.m.) value in adult seals was 8.7 +/- 0.3% (N=6), while juveniles and pups (with lower heme-protein ...

Oxygen and carbon monoxide adsorption studies were carried out in ultra high vacuum on vapor deposited polycrystalline platinum films with a |111| fiber axis and on monocrystalline (111) palladium films. The techniques of Auger electron spectroscopy (AES), electron loss spectroscopy (ELS) and thermal desorption spectroscopy (TDS) were used. A new form of electron diffraction pattern, namely Kikuchi rings, was also observed from these films. Oxygen adsorption studies on the Pt films showed the existence of two forms of adsorbed oxygen. TDS of carbon monoxide adsorbates showed only one peak and resulted in the development of a new loss line in the ELS spectrum. Electron beam irradiation decomposed the adsorbed CO resulting in a shape change for the carbon Auger line. The rate of reaction for carbon monoxide oxidation was found to be temperature dependent, thereby supporting the Langmuir-Hinshelwood mechanism for CO oxidation. Transmission electron microscope (TEM) replica studies of vapor deposited (111)

A pilot-scale, pre-anoxic-anaerobic oxidation ditch was used in this study to treat municipal wastewater with limited carbon source. A novel return activated sludge (RAS) pre-concentration tank was adopted for improving the phosphorus removal efficiency and the effects of RAS pre-concentration ratio were studied. Under the optimal operational condition, the suspended total phosphorus (STP) and the total phosphorus (TP) removal efficiencies were around 58.9% and 63.9% respectively and the effluent PO3-4 -P was lower than 0.8 mg•L−1. The reason is that with the optimal RAS pre-concentration ratio, nitrate is completely removed with endogenous carbon source and the secondary phosphorus release is strictly restrained in the pre-anoxic tank. Therefore, the anaerobic phosphorus release and the carbon source uptake by phosphorus accumulation organisms (PAOs) in the sludge, which are extremely important to the phosphorus removal process, can be fully satisfied. Furthermore, the oxidation-reduction ...

sup13)C nuclear magnetic resonance spectroscopy was used to investigate citrate-glucose cometabolism in nongrowing cell suspensions of the wine lactic acid bacterium Leuconostoc oenos. The use of isotopically enriched substrates allowed us to identify and quantify in the end products the carbon atoms derived from each of the substrates supplied; furthermore, it was possible to differentiate between products derived from the metabolism of endogenous carbon reserves and those derived from external substrates. Citrate-sugar cometabolism was also monitored in dilute cell suspensions for comparison with the nuclear magnetic resonance results. A clear metabolic shift of the end products from glucose metabolism was observed when citrate was provided along with glucose: ethanol was replaced by acetate, and 2,3-butanediol was produced. Reciprocally, the production of lactate and 2,3-butanediol from citrate was increased in the presence of glucose. When citrate was cometabolized with glucose, a 10-fold ...

Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor challenging those bacteria in their natural marine habitats. Here we report on the analysis of storage lipid biosynthesis in A. borkumensis strain SK2. Triacylglycerols (TAGs) and wax esters (WEs), but not poly(hydroxyalkanoic acids), are the principal storage lipids present in this and other hydrocarbonoclastic bacterial species. Although so far assumed to be a characteristic restricted to gram-positive actinomycetes, substantial accumulation of TAGs corresponding to a fatty acid content of more than 23% of the cellular dry weight is the first characteristic of large-scale de novo TAG biosynthesis in a ...

Fingerprint Dive into the research topics of Chiroptical properties of anionic and cationic porphyrins and metalloporphyrins in complex with left-handed Z-DNA and right-handed B-DNA. Together they form a unique fingerprint. ...

Materials. [3H]Methotrexate (46.8 Ci/mmol) and [3H]folate (30 Ci/mmol) were obtained from Moravek Biochemicals (Brea, CA), [3H]cimetidine (25.0 Ci/mmol) was from GE Healthcare (Chalfont St. Giles, UK) and [3H]estrone sulfate (57.3 Ci/mmol) and [3H]taurocholate (1.2 Ci/mmol) were from PerkinElmer Life Sciences, Inc. (Boston, MA). Folate, methotrexate, 5-methyltetrahydrofolate, folinate, geneticin, and sulfasalazine were obtained from Sigma-Aldrich Co. (St. Louis, MO). Zinc protoporphyrin IX (Calbiochem, San Diego, CA) was obtained from Merck (Darmstadt, Germany). Restriction enzymes were obtained from TOYOBO (Tokyo, Japan). All other reagents were of analytical grade and commercially obtained.. Cell Culture. Human embryonic kidney (HEK)293 and MDCKII cells were maintained at 37°C and 5% CO2 in Dulbeccos modified Eagles medium with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin.. Isolation of hPCFT/HCP1. The cDNA of hPCFT/HCP1 was cloned from the human intestine total ...

Porphyrins are molecular complexes that are critical for the proper functioning of many important biological macromolecules such as hemoglobin and myoglobin. The goal of this research is to monitor the transformations and differences that occur with the addition of metals and ligands to a model porphyrin compound called tetraphenylporphyrin (TPP). During this work, a series of metalloporphyrin complexes were prepared, with changes to both metal centers and ligand attachments at those centers. Specifically, iron, zinc, cobalt and manganese metalloporphyrins have been synthesized and identities verified via electronic absorption spectroscopy. Ligand binding studies have begun with the addition of piperidine and will continue on with additional molecules such as: pyrrolidine, imidazole, as well as secondary amines. About the author: Frank is a senior biochemistry major who plans on attending graduate school followed by medical school after his time at Valparaiso University.

The CH-42 gene of Arabidopsis codes for a subunit of magnesium chelatase which is required for the biosynthesis of the green photosynthetic pigment chlorophyll (Koncz et al., 1990). Homozygous mutants of this locus are typically albino or yellow in phenotype and are recessive to the dominant wild-type allele in heterozygotes (Furner et al., 2008). A wild-type copy of the genomic copy CH-42 gene supplied on a T-DNA somewhere in the genome is usually sufficient to complement the ch-42 mutation at the normal locus (Furner et al., 2008). Experiments with X-irradiated hemizygotes suggest that small sectors of ch-42 tissue have a cell autonomous yellow phenotype (Furner et al., 1996). Silencing of the CH-42 gene should result in decreases in magnesium chelatase and chlorophyll and increases in the amount of yellow chlorophyll deficient tissue.. Hairpin constructs directed against the 3 end of the CH-42 gene result in a dominant yellow phenotype (Daxinger et al., 2008). The Ubi42 line contains an ...

The synthesis is reported of a series of metalloporphyrins (and the corresponding free-base porphyrin), mono-meso-substituted with a bipyridyl groupvia an amide link at the 4-position of one phenyl group: [Re(CO)3(Pic)Bpy-MTPP][OTf], where M = Mg, Zn, Pd or 2H, Pic = 3-picoline, Bpy = 2,2′-bipyridine, TPP =

இருகுளோரின் ஓராக்சைடு (Dichlorine monoxide) ஓராக்சைடு என்பது Cl2O என்ற மூலக்கூற்று வாய்ப்பாடு கொண்ட ஒரு கனிம வேதியியல் சேர்மமாகும். இச்சேர்மம் முதன்முதலில் 1834 ஆம் ஆண்டில் அண்டோயின் செரோம் பலார்டு என்பவரால் தொகுப்பு முறையில் தயாரிக்கப்பட்டது.கேலூசக்குடன் இணைந்து இச்சேர்மத்தின் இயைபையும் இவர் உறுதி செய்தார். பண்டைக் காலத்தில் இச்சேர்மம் பெரும்பாலும் குளோரின் ஓராக்சைடு[2] என்றே ...