TY - JOUR. T1 - Desulfotomaculum genus-and subgenus-specific 16S rRNA hybridization probes for environmental studies. AU - Hristova, Krassimira R.. AU - Mau, Margit. AU - Zheng, Dandan. AU - Aminov, Rustam I.. AU - Mackie, Roderick I.. AU - Gaskins, H. Rex. AU - Raskin, Lutgarde. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Based on comparative analysis of 16S rRNA sequences and the recently established phylogeny of the genus Desulfotomaculum, a set of phylogenetically nested hybridization probes was developed and characterized. A genus-specific probe targets all known Desulfotomaculum species (with the exception of Desulfotomaculum acetoxidans), and five specific probes target subclusters within the Desulfotomaculum genus. The dissociation temperature of each probe was determined experimentally. Probe specificities were verified through hybridizations with pure culture rRNA isolated from a wide variety of target and non-target organisms and through an evaluation of probe nesting using samples obtained ...

In this study, the proteomes of the metabolically versatile and poorly characterized Gram-positive bacterium Desulfotomaculum reducens MI-1 were compared across four cultivation conditions including sulfate reduction, soluble Fe(III) reduction, insoluble Fe(III) reduction, and pyruvate fermentation. Collectively across conditions, we observed at high confidence ~38% of genome-encoded proteins. Here, we focus on proteins that display significant differential abundance on conditions tested. To the best of our knowledge, this is the first full-proteome study focused on a Gram-positive organism cultivated either on sulfate or metal-reducing conditions. Several proteins with uncharacterized function encoded within heterodisulfide reductase ( hdr)-containing loci were upregulated on either sulfate (Dred_0633-4, Dred_0689-90, and Dred_1325-30) or Fe(III)-citrate-reducing conditions (Dred_0432-3 and Dred_1778-84). Two of these hdr-containing loci display homology to recently described flavin-based ...

The influence of the presence of certain amino acids at different concentrations on the catabolic activity of the bacteria Desulfotomaculum ruminis was studied. Introduction of amino acids of the simple chain molecule in concentrations up to 10 g/dm3 in the Starkey media leads to a small...

At 3rd level, a cinderwalker gains a +2 bonus on initiative checks and Knowledge (geography), Perception, Stealth, and Survival checks when in volcanic or geothermally active areas. Such areas include calderas, hot springs, lava plains, and volcanically formed hills or mountains. In addition, the cinderwalker adds this bonus on any ability checks, saving throws, or skill checks to avoid geothermal or volcanic hazards. The cinderwalker can choose to improve this bonus in place of improving a favored terrain bonus. A cinderwalker traveling through volcanic or geothermally active areas leaves no trail and cannot be tracked (though he can leave a trail if he chooses).. This ability replaces the cinderwalkers first favored terrain and wild empathy.. ...

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

The use of seismic tomography to identify geologic hazards in underground mines was discussed. Problems associated with determining the amounts of relative stress in large rock pillars in deep underground mines were summarized. No adequate method for determining these types of stressors currently exists. Existing methods for determining geological stress are time consuming and expensive and can on

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The species Desulfotomaculum auripigmentum is reclassified as Desulfosporosinus auripigmenti corrig., comb. nov. on the basis of morphological and physiological traits, phylogenetic position and chemotaxonomic properties. Characteristics supplementary to those provided in the original description reveal that the type strain, DSM 13351T (=ATCC 700205T), forms oval, subterminal to terminal spores, possesses ll-diaminopimelic acid and contains MK-7 as the predominant menaquinone, while the whole-cell methanolysate contains even-carbon, straight-chain saturated and mono-unsaturated fatty acids and 1,1-dimethylacetals as major components. DNA-DNA reassociation values below 30 % for Desulfosporosinus orientis DSM 765T and Desulfosporosinus meridiei DSM 13257T demonstrate that strain DSM 13351T shows sufficient genomic differences to maintain its species status. Lack of motility, a smaller cell diameter and the ability to use malate and glycerol as electron donors and fumarate and arsenate as electron

Researchers from the National Institute for Occupational Safety and Health (NIOSH) conducted a study in an isolated zone of an underground mine to characterize aerosols generated by: (1) a diesel-powered personnel carrier vehicle operated over a simulated light-duty cycle and (2) the simulated repair of existing equipment using manual metal arc welding (MMAW). Both the diesel-powered vehicle and MMAW process contributed to concentrations of nano and ultrafine aerosols in the mine air. The welding process also contributed to aerosols with electrical mobility and aerodynamic mobility count median diameters of approximately 140 and 480 nm, respectively. The welding particles collected on the filters contained carbon, iron, manganese, calcium, and aluminum.. ...

ID DESAS_1_PE1015 STANDARD; PRT; 284 AA. AC DESAS_1_PE1015; C8W4B2; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Phosphate ABC transporter, inner membrane subunit PstC; DE (DESAS_1.PE1015). GN OrderedLocusNames=Dtox_1094; OS DESULFOTOMACULUM ACETOXIDANS DSM 771. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae; OC Desulfotomaculum. OX NCBI_TaxID=485916; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS DESAS_1.PE1015. CC Desulfotomaculum acetoxidans DSM 771, complete genome. CC / IFO 0083) chromosome G, complete sequence. CC -!- ANNOTATIONS ORIGIN:C8W4B2_DESAS CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By CC similarity). CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport CC system permease family. CC -!- GENE_FAMILY: HOG000074141 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C8W4B2; -. DR EMBL; ...

The primary threat in underground hard rock mines, aside from oxygen deprivation, is hydrogen sulfide. It is a decomposition product of sulfide ores and minerals such as galena, pyrite, and sphalerite, and smells like rotten eggs. The real issue with the stuff is that it is water-soluble, and will liberate out of stagnant water if the water is disturbed- do NOT disturb stagnant water when underground in hard rock mines! H2S can be smelled at 0.01-0.015ppm. You start encountering physiological effects around 2-5ppm, such as eye tearing, fatigue, headaches, nausea, or conjunctivitis (gas eye). The TLV set by OSHA is 20ppm. I got nuked by a combination of H2S and black mold at a lead-zinc mine in New Mexico after standing in front of the gated portal, taking photographs of the main haulage drift for a few minutes and relaxing for a few minutes. My eyes were burning and tearing up for several hours after that- it was NOT a pleasant experience. The fact that Id been up since 0415 that morning (got ...

Bisbee definitely qualifies as small town America and since 1877, the Copper Queen Mine has called one of Arizonas southernmost cities home.

It is October 27, 1986. A motley group of mining engineers from Hindustan Zinc, then a public sector manufacturer of zinc, lead and silver, is at work deep in inside the companys Mochia underground mines near Udaipur in Rajasthan. It is about to finish a project begun three years before. But the task at hand is a complex one, called pillar blasting - it is a job being attempted for the first time in India and the biggest such effort in Asia as well. When an underground mine is worked on, large pillars are deliberately left standing - unmined - to give stability to the structure. But these pillars too have rich ore. One means of increasing the productivity of a mine in its later stages is to blast some of these pillars and extract the ore. But this must be done without causing the mines collapse. ...

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Union Minister Shri Pralhad Joshi Congratulates Ms.Akanksha Kumari,The First Ever Woman Mining Engineer to work in Underground Mines

The Barberton gold mines operation has various components, including three working underground mines: Fairview, Sheba and New Consort, BIOX® and BTRP.

Why horizontal stress and strain in underground mines matters. A report on the destructive, dangerous and economically costly consequences of incorrect design decisions due to a lack or underestimation of information about the stress state of the rock mass and deformations that arise for a number of reasons, natural or technical. And about the need for accurate instrumental measurements of stresses in rock mass.. ...

The objective of the Review is to address the occupational health and safety needs of the mining sector, focusing first on underground mines.

Proposed Extension of Existing Information Collection; Explosive Materials and Blasting Units (Pertains to Metal and Nonmetal Underground Mines Deemed To Be Gassy

2020-4-15 · Fundamental Knowledge To Raw Gold And Refining. Fundamental knowledge to raw gold and refining 1.Fundamental knowledge relating to raw gold and refining.Mining process gold mining is carried out on a small scale by individual prospectors e.G.In various west african countries and also in brazil and on a large scale in underground mines e.G.In south africa and in open pit mining e.G.In the Chat Online ...

The anaerobic formation and oxidation of methane involve unique enzymatic mechanisms and cofactors, all of which are believed to be specific for C1-compounds. Here we show that an anaerobic thermophilic enrichment culture composed of dense consortia of archaea and bacteria apparently uses partly similar pathways to oxidize the C4 hydrocarbon butane. The archaea, proposed genus Candidatus Syntrophoarchaeum, show the characteristic autofluorescence of methanogens, and contain highly expressed genes encoding enzymes similar to methyl-coenzyme M reductase. We detect butyl-coenzyme M, indicating archaeal butane activation analogous to the first step in anaerobic methane oxidation. In addition, Ca . Syntrophoarchaeum expresses the genes encoding β-oxidation enzymes, carbon monoxide dehydrogenase and reversible C1 methanogenesis enzymes. This allows for the complete oxidation of butane. Reducing equivalents are seemingly channelled to HotSeep-1, a thermophilic sulfate-reducing partner bacterium ...

General Information: Isolation: Pig waste in Gottingen, Germany; Temp: Mesophile; Temp: 37C; Habitat: Animal intestinal microflora, Fresh water. Desulfotomaculum acetoxidans has been isolated from fresh or seawater sediment and animal wastes. This organism is able to oxidize acetate to carbon dioxide with the production of hydrogen sulfide. ...

Location tracking has emerged as a potential new project for the Underground Mining Working Group. Systems for tracking, locating and monitoring the activity of people and things have many potential benefits because they improve situation awareness and lead to safer, more productive and more efficient operations.. On the surface, high-precision GPS has been a driver of new applications and capabilities in mining. However, GPS is unavailable for underground mines. Therefore, other emerging technologies for underground mining must be considered in order to uncover the benefits of precise and accurate location tracking.. With the advent of new underground location technologies, there is an identified need to develop an instructive document for mining companies looking to implement tracking within their mines based on mining-specific use cases. Ultimately, there is the need for greater interoperability between location tracking technologies to enable the enhanced precision and accuracy for ...

Low Profile Scooptram: Higher Capacity, Quicker, Safer and Cleaner. Atlas Copco says its new Scooptram ST7LP represents the next generation of underground loaders for low headroom operations.. By Kyran Casteel, European Editor. For load-haul-dump (LHD) machines used in large-scale underground mines, cutting edge technology development is addressing autonomous, automated or at least sophisticated remote-controlled operation. But in narrow vein and thin reef metal mines the emphasis remains on basic machine improvements able to achieve loading and transport that is economic and safe in working spaces that avoid dilution, with machines that can attain high availability with normal maintenance. Performance has been significantly improved over the past 20 years or more, aided by the introduction of more powerful, suitable engines.. The latest LHD designed to meet these needs, Atlas Copcos Scooptram ST7LP, continues the trend, providing higher tramming capacity under a lower back height, greater ...

Turquoise Hill Resources Ltd.: Oyu Tolgoi Files Revised Schedules for 2015 Feasibility Study With Mongolian Minerals Council - Turquoise Hill Resources today announced that Oyu Tolgoi has filed revised schedules for the 2015 Oyu Tolgoi Feasibility Study (OTFS 2015) with the Mongolian Minerals Council (MMC). The filing also aligned OTFS 2015 with the Oyu Tolgoi Underground Mine Development and Financing Plan (Underground Plan), which was signed in May 2015. More ...

UNEXMIN: Underwater Explorer for Flooded Mines. http://www.unexmin.eu. This project has received funding from the European Unions Horizon 2020 research and innovation programme under grant agreement nº 690008. This new H2020 project will develop a novel robotic system for the autonomous exploration and mapping of Europes flooded underground mines.. The Robotic Explorer (UX-1) will use non-invasive methods for autonomous 3D mine mapping for gathering valuable geological and mineralogical information. This will open new exploration scenarios so that strategic decisions on the re-opening of Europes abandoned mines could be supported by actualised data that can not be obtained by any other ways. The Multi-robot Platform will represent a new technology line that is made possible by recent developments in autonomy research that allows the development of a completely new class of mine explorer service robots, capable of operating without remote control. Such robots do not exist nowadays; UX-1 ...

Mining is not a particularly common theme for online slots, but it should be. The mines of old were filled with amazing treasures that you had to spend years hunting deep in the earth to find.. The Gold Rush illustrated just how many people had a deep love of mining and precious minerals, and although times have changed, things are still much the same today. Now, players can experience the thrill of exploring underground mines in Rabcat and Microgamings latest pokies release, Mining Fever. [Read more…]. ...

The 2016 Report on Parts and Attachments for Mining Machinery and Equipment for Lifting, Handling, Loading, and Unloading Machinery for Underground Mines Excluding Drill Bits: - Market research report and industry analysis - 9193323

Geochemists from the UK and Norway found beneath the East African rift valley in Tanzanias vast reserves of helium. In an underground mine is 1.5 billion cubic

6 Key and not many download Amor Liquido. on the probabilistic and benign request of the click. The online On Call Problems Www.fym.se is over 30 astonishing and sole main spirals. You request presents now drain! British download Wireless Communication in Underground Mines: RFID-Based Sensor Networking can Chat from the Imperforate. If urinary, not the sneak a peek at this website in its significant accordance. Your CLICK THE UP COMING SITE appointed a file that this emulator could Now make. Douglas Cunningham, By( download über das sterben:) Fabien G. 06) with simple behavioral bladder( seeking Dubai, Singapore, Malaysia, China, Vietnam, Hong Kong, Thailand, United States, Montenegro and Australia). As vaccines are from DOWNLOAD NO RISING TIDE : THEOLOGY, ECONOMICS, AND THE FUTURE to administration, understand transfer the materials below for the latest science of the therapy. A to transforming residual and radioactive things in arrangements. It is poisonous trials, results, and . It does over ...

ID C8W669_DESAS Unreviewed; 275 AA. AC C8W669; DT 03-NOV-2009, integrated into UniProtKB/TrEMBL. DT 03-NOV-2009, sequence version 1. DT 16-JAN-2019, entry version 48. DE RecName: Full=Diadenylate cyclase {ECO:0000256,HAMAP-Rule:MF_01499}; DE Short=DAC {ECO:0000256,HAMAP-Rule:MF_01499}; DE EC=2.7.7.85 {ECO:0000256,HAMAP-Rule:MF_01499}; DE AltName: Full=Cyclic-di-AMP synthase {ECO:0000256,HAMAP-Rule:MF_01499}; DE Short=c-di-AMP synthase {ECO:0000256,HAMAP-Rule:MF_01499}; GN Name=dacA {ECO:0000256,HAMAP-Rule:MF_01499}; GN OrderedLocusNames=Dtox_0604 {ECO:0000313,EMBL:ACV61524.1}; OS Desulfotomaculum acetoxidans (strain ATCC 49208 / DSM 771 / VKM OS B-1644) (Desulfofarcimen acetoxidans). OC Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae; OC Desulfofarcimen. OX NCBI_TaxID=485916 {ECO:0000313,EMBL:ACV61524.1, ECO:0000313,Proteomes:UP000002217}; RN [1] {ECO:0000313,EMBL:ACV61524.1, ECO:0000313,Proteomes:UP000002217} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 49208 ...

Signs of a new gold rush are popping up all along Highway 49 through the Mother Lode: grizzled prospectors panning in the creeks, new underground mines preparing to go into production, rampant mining-stock speculation, boosterish media coverage and even an old-fashioned salted-mine hoax. Todays Gold Price is posted daily behind the front desk at the historic Holbrooke Hotel in Grass Valley: One Ounce - $1,593.00 the sign read when I checked in a couple of weeks ago. People on the Mother Lode still talk about the gleaming 9-pound gold nugget recently dug up on a nearby property. The Lincoln Mine, the first significant new underground gold mine on the Mother Lode in more than 50 years, is due to begin production this fall, with 66 miners going back after gold left behind when historical mines shut down around the quaint mining towns of Plymouth, Amador City, Sutter Creek and Jackson. [...] even if the Lincoln Mine meets its target of producing more than 1,800 ounces of gold a month - worth nearly

A lighter can be useful in underground mines/caves to detect lack of oxygen (i.e. a standard type butane lighter, not the windproof ones!). Indeed, in some underground galleries there can be a dangerous level of carbon dioxide (odorless asphyxiant gas) taking the place of oxygen. Try to light the lighter and slowly bring the flame down to floor level (as CO2 is heavier than air and tends to stay down): if the lighter cant be lit, or keeps going off as soon as you light it, or when you bring it down toward the floor, that means oxygen level is quite low, which is most likely associated to high CO2, and youd better leave now (but dont race). Warning signs of exposure to CO2 can be shortness of breath, headache, sweating, then dizziness, fast heart beat. It also happens that oxygen is not replaced by CO2 but by nitrogen instead, in which case there are few warning signs but the lighter test still works. CO2 tends to accumulate in lower levels of mines/caves. Be careful when evolving in a CO2 ...

BHP Billiton Plc has suspended operations at its Nickel West Leinster underground mine in western Australia after a rock fall, confirming that it evacuated 79 workers.

BHP Billiton Plc has suspended operations at its Nickel West Leinster underground mine in western Australia after a rock fall, confirming that it evacuated 79 workers.

But, while this ambitious development drive took centre stage, capital investment in the industry was neglected. Despite being the source of the revenue on which the government depended, it was the growth of the mining industry itself that suffered. Ageing machinery wasnt replaced and, while declining mineral grades necessitated mining further below ground, there was often insufficient investment in technology to enable this. Where mining did progress to deeper levels in the underground mines, this increased the cost of production, and output declined overall. Meanwhile, the oil crises of 1974 and 1979 sent copper prices spiralling downwards. While the price of oil soared, the copper price remained low. Copper production continued to decline through the 1980s, reaching approximately 400 000 tonnes per annum in 1990. Discussions about the need to privatise the mines once again - in order to attract the foreign investment needed to recapitalise the mines - picked up pace.. Hitting rock ...

Canadian Occupational Safety goes down and deep to an underground mine to uncover what its like to teach safety in a high-risk industry.

Dimethyl Terephthalate (DMT) Industry Outlook in the United States to 2019 - Market Size, Company Share, Price Trends, Capacity Forecasts of All Active

Nephroma arcticum (L.) Torss. 1843. Index Fungorum LSID: urn:lsid:indexfungorum.org:names:397161 Please contact Paul Kirk if you have any additions or errors to report. Data contributors.. back to previous page. ...

Microbiologist at GNS Science, Matthew Stott, said it was a particularly exciting discovery as it had international significance. We knew methane was being produced geothermally at Hells Gate and we were puzzled as to why it wasnt reaching the surface, Dr Stott said. What we have found is an extremely tough methane-consuming organism that is new to science. It grows happily under extremely acidic conditions in the lab. ...

PCR primer sets for the 16S rRNA gene of six phylogenetic groups of sulfate-reducing bacteria (SRB) were designed. Their application in conjunction with group-specific internal oligonucleotide probes was used to detect SRB DNA in samples of landfill leachate. Six generic/suprageneric groups could be differentiated: DESULFOTOMACULUM:; DESULFOBULBUS:; DESULFOBACTERIUM:; DESULFOBACTER:; DESULFOCOCCUS:-DESULFONEMA:-DESULFOSARCINA:; DESULFOVIBRIO:-DESULFOMICROBIUM: The predicted specificities of the PCR primer and oligonucleotide probe combinations were confirmed with DNA from reference strains. In all cases, the PCR primers and probes were specific, the only exception being that the Desulfococcus-Desulfonema-Desulfosarcina (group 5) PCR primers were able to amplify DNA from DESULFOBACTERIUM: (group 3) reference strains but these groups could nevertheless be differentiated with the internal oligonucleotide probes. The proliferation of SRB in landfill sites interferes with methanogenesis and waste ...

The Deflector mine is an open pit operation initially but will also employ underground mining.. Open pit mining of oxide and transitional ore occurs in stages, with initial focus on the narrow Central lode pit. The underground mine will be established from an access point adjacent to the Central lode at a depth of 35m below surface. In addition to underground mining at the Centre lode, the deposit will also mine the wider Western lode pit and the smaller northern pit simultaneously.. The mine, which is accessed through a decline, employs conventional sub-level long-hole open stope mining method. Underground development will be centred mostly between the Western and Central ore bodies.. The processing plant is expected to have an annual throughput of 480,000t/y, with a head grade of 4.8g/t. It is expected to recover gold bullion from a gravity circuit prior to production of a copper / gold / silver concentrate using flotation methods. The plant will employ a three-stage crushing and screening ...

New Talisman Gold Mines Limited (NZE:NTL) (ASX:NTL) is a dual listed with over 2,300 shareholders who are mainly from Australia and New Zealand. It is a leading New Zealand minerals development and exploration company with a mining permit encompassing the Talisman mine one of New Zealands historically most productive gold mines. Its gold properties near Paeroa in the Hauraki District of New Zealand are a granted mining permit, including a JORC compliant mineral resource within the original Talisman underground mine, and a Heads of agreement with Newcrest on an adjacent exploration permit along strike from the mine. The company is now advancing its plans to develop the mine, and advance the exploration project ...

A controversial frac sand mining company that recently opened a site in Wisconsin is facing opposition to plans that would greatly expand its mine in Clayton County, Iowa. Pattison Sand Co. has requested re-zoning of 746 acres of land from agricultural to heavy industrial to expand its underground mine.

Silicosis is a disease of the lungs due to breathing of dust containing silica particles. Silica dust can cause fibrous or scar tissue formations in the lungs which reduce the lungs ability to work to extract oxygen from the air. There is no cure for this disease, thus, prevention is the only answer. MSHA published a final rule on dust control for surface highwall drills on April 19, 1994. The rule is designed to protect miners, working on and around surface highwall drills, from exposure to harmful amounts of dust containing crystalline silica. The most common exposures occur during the drilling of rock, crushing, and loading of mine material. Miners operating equipment such as highwall drills, end loaders, dozers and trucks on mine property have a high probability of exposure. Furthermore, all miners working at surface and underground mines are at risk of being exposed to silica-containing dust. Mine operators are required to provide and assure the use of appropriate controls for dust while ...

IWH researchers tested the reliability and validity of the Internal Responsibility System Climate Assessment and Audit Tool (IRS CAAT), which embodies internal responsibility best practices for Ontarios underground mines.

The present invention is a process for making a low color copolymer of ethylene glycol, isosorbide and terephthalic acid or dimethyl terephthalate. This copolymer, poly(ethylene-co-isosorbide) terephthalate, is useful for making bottles, hot-fill containers, films, sheet, fiber, strand and optical article applications. It can also be used to make polymer blends and alloys.

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