Late Embryogenesis Abundant proteins (LEAPs) comprise several diverse protein families and are mostly involved in stress tolerance. Most of LEAPs are intrinsically disordered and thus poorly functionally characterized. LEAPs have been classified and a large number of their physico-chemical properties have been statistically analyzed. LEAPs were previously proposed to be a subset of a very wide family of proteins called hydrophilins, while a domain called WHy (Water stress and Hypersensitive response) was found in LEAP class 8 (according to our previous classification). Since little is known about hydrophilins and WHy domain, the cross-analysis of their amino acids physico-chemical properties and amino acids usage together with those of LEAPs helps to describe some of their structural features and to make hypothesis about their function. Physico-chemical properties of hydrophilins and WHy domain strongly suggest their role in dehydration tolerance, probably by interacting with water and small polar
Briefly, eyes and lids from mice in each group (n = 3 per group) were surgically excised and embedded in OCT compound (VWR, Swannee, GA) and flash frozen in liquid nitrogen. Sagittal 8-μm sections were cut with a cryostat (HM 500; Micron, Waldorf, Germany) and placed on glass slides that were stored at −80°C. They were fixed with either cold acetone (involucrin, Sprr-2, and Tg-1) at −20°C for 5 minutes or with methanol (Sprr-1a, and Muc5AC+Sprr-1a) at 4°C for 5 minutes and then permeabilized with PBS containing 0.1% Triton X-100 for 10 minutes. After blocking with 20% normal goat serum in PBS for 60 minutes (except for involucrin and Muc5AC where 20% horse normal serum was used), primary polyclonal antibodies (dilution 1:100 for all of them, except for involucrin, which was 1:20) were applied, and the sections were incubated for 1 hour at RT. When dual labeling was intended, Alexa-Fluor 488-conjugated goat anti-rabbit IgG and Alexa-Fluor 633-conjugated donkey anti-goat IgG (1:300) ...
Berikut ini kuliah-karyawan.info sampaikan mengenai Lund University PhD Scholarship on Molecular Basis of Heat and Desiccation Tolerance of Bacterial Cells and Spores
SUMMARY: Two different organisms were dried in normal rabbit serum and in the presence of specific antibody. Reconstitution of the dried products showed that the presence of antibody did not affect the viability, agglutinability or the absorptive capacity of the bacteria.
Some tissues of most higher plant species are capable of tolerating extremes of desiccation. Typically, these tissues are found within the developing seed (Kermode et al., 1986), but for some species...
I am suffering from back pain since a long time. Recently I had my M.R.I. scan which shows |b|disc desiccation at L3/L4 and L4/L5|/b| with rest of the intervertebral discs being normal. The doctor advised me to take Tab. Nimesulide BP 100mg in the morning and Tab. Celepra 20 (citalopram hydrobromide)in the evening, for two months. But there is not much improvement. Doctor please advise if I should continue this treatment? Is there any slip disc in the report and what is the meaning of desiccation?
Oil dehydration, also referred to as vacuum dehydration, these process is specially designed in order to remove the light contaminants from the Used Lube Oil.
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BETX stands for benzene, ethylbenzene, toluene, and xylene, a group of compounds that all belong to the broader category of Hazardous Air Pollutants (HAPs). Benzene and ethylbenzene are known carcinog
These are the roo bites and the lamb puffs. Jump Your Bones Pet Treats uses an exclusive DSA dehydration process that uses a low temperature and low humidity. This process allows the product to retain its natural aroma. Hence, the reason why Tibby thought they smelled AMAZING! Also, Jump Your Bones Pet Treats are virtually FAT FREE, because of the process used to dry them. Their web site is pretty neat, it shows the whole drying process. Very state of the art! Because of the DSA dehydration process the treats retain the same natural enzymes and nutritional value as the fresh product ...
These are the roo bites and the lamb puffs. Jump Your Bones Pet Treats uses an exclusive DSA dehydration process that uses a low temperature and low humidity. This process allows the product to retain its natural aroma. Hence, the reason why Tibby thought they smelled AMAZING! Also, Jump Your Bones Pet Treats are virtually FAT FREE, because of the process used to dry them. Their web site is pretty neat, it shows the whole drying process. Very state of the art! Because of the DSA dehydration process the treats retain the same natural enzymes and nutritional value as the fresh product ...
One area that is critical to the recovery process is rehydrating. Rehydrating not only replenishes fluids lost during exercise, but it also aids in replenishing the electrolytes that were also lost. Keep in mind: drinking too much too fast can degrade the potential gains of the workout. Heres why: over time your body has developed a strong immune system that helps repair itself when it is under the stresses of exercise. Therefore, naturally, your body begins repairing itself, slowly rehydrating, and continuing to hydrate throughout the day.. Editors note: recover faster when you follow this long training run recovery timeline from the Austin Marathon!. Nuun Hydration is our Official Hydration partner. Head over to www.nuunlife.com to stock up on your hydration supply and train with what will be on course. Save 25% with the code below!. ...
Question - Have tingling and numbness on leg. Experiencing impotence. Has disc desiccation. Related?. Ask a Doctor about when and why MRI is advised, Ask an Orthopaedic Surgeon
1XO8: Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana, a cellular stress-related protein
The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolisstrain T902, bearing a plasmid with...
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I am having alot of pain ever day it never goes away or gets better . I will try too not make this too long but I am in need of some help ! I am 35 when I was 28 I was working as a home health nurse and my lower back went out from L1-L4 -S1 I under went back surgery 2 rods 6 bolts and a cage and that is when all my prob. started too take place in 2006 I had a MRI and this is what it said at that time herniated disc,disc desiccation,focal fibrosis at S1-L5 nerve root damage with nerve impingement,stenosis, and in 2009 I had a MRI done and this is what it said stenosis at T7-T11 adnormal central in lower Tspine ,disc space narrowing,disc desiccation,hypertrophy,at T7-T11 disc ostephyte,canal stenosis at same area syrinx/hydrosyringomyelia, the same day they did MRI of the C spine and this is what it said cervical spondylosis,stenosis at C3-C5,disc desiccation at C3-C5,disc osteophyte and stenosis at C3-C5, they did a repeat of the MRI this past week I have not got anything back on it as of ...
I thought this article at Howstuffworks was appropriate just after the all day drinking fest that is St. Patricks Day for many.. According to the article, a hangover from a heavy night (and/or day) of drinking is mainly due to dehydration.. The dehydration process begins with a chemical reaction in the brain; specifically the pituitary gland. This reaction causes less vasopressin to be released from the pituitary gland, which in turn causes the kidneys to send water directly into the bladder (rather than reabsorbing it).. So why do hangovers cause headaches? Apparently the massive amount of dehydration by the morning causes the bodys organs to steal water from the brain.. According to the article, this causes the brain to decrease in size and pull on the membranes that connect the brain to the skull, resulting in pain. This cannot be good for neuronal health!. Based on this information it seems that the best way to avoid a hangover is to drink plenty of water along with those heavy booze. ...
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The thermal stability properties of poly (ethylene-co-vinyl acetate) composites have been studied in support of our core programmes in materials qualification and life assessment. The material is used as a binder phase for boron particles in highly filled (70 wt %) composites. Our studies show that the uncured resin readily accumulates acetic acid through hydrolysis of the pendent acetate groups which alters the acidity (pH) of the material. Thermal desorption studies in combination with gas-chromatography-mass spectrometry show that the resin readily evolves acetic acid when thermally aged to temperatures up to 75°C. Gel Permeation Chromatography (GPC) suggests that thermal ageing induces a gradual reduction in resin molecular weight and confirms the susceptibility of the material to chain scission. Heating at elevated temperatures in excess of 300oC is required to induce significant changes in the carbon skeleton through deacetylation and dehydration processes and the production of ...
Late-embryogenesis is characterised by the accumulation of large amounts of storage proteins, the acquisation of desiccation tolerance and the induction of dormancy. Genetic studies demonstrated that these processes are controlled by the transcription factors LEC1, FUS3 and ABI3. The present study was focused on the molecular characterisation of FUS3, LEC1 and ABI3 in an attempt to clarify the interaction of these transcription factors and their role in late-embryogenesis. To investigate the potential interaction between FUS3, ABI3 and LEC1 and their function on the activation of seed-storage protein promoters a transient expression system in tobacco protoplasts was established. With the help of this system it was shown that the FUS3- and ABI3-gene products can induce both independently and dependently of each other storage protein promoters. The FUS3 and ABI3 mediated increase in activity of storage protein promoters could be strongly reduced when a conserved sequence element, the RY motif, was ...
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LEA proteins are synthesized late during embryogenesis in plant seed development. Structurally related proteins have also been found in vegetative plant tissues under various stress conditions and in desiccation tolerant invertebrates and microbes. They thus seem to be universal dehydration stress proteins in living cells, with the exception of vertebrates. Their biological functions in stress tolerance, however, are unclear. Most of these proteins have no stable secondary structure in solution, i.e. they belong to the large group of intrinsically disordered proteins (IDPs). We are concentrating our efforts on the largest LEA protein family (Pfam LEA_4) that has 18 members. We produce proteins of interest recombinantly in E. coli and use mainly fluorescence, far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) spectroscopy for structural and functional characterization. To study the interactions with membranes, we use artificial lipid vesicles (liposomes) that can be produced ...
After vigorous exercise rehydrating is important but beware drinking water is replacing the water not the sodium. Too little sodium can also kill.
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Surgical Humidification reduces cellular desiccation and evaporative cooling during laparoscopic and open surgery. This has been shown to significantly reduce the risk of postoperative complications and their associated costs.
The young leaves are sticky and collect dust and sand grains to protect them against desiccation (the genus name means sand bearer). The small plant is accompanied by Sarcocaulon (Monsonia) multifidum ...
ninth download TcL Scripting for Cisco IOS knowledge and desiccation of conditions. long, this range is other with the periphyton of Mosaad et al. All of these steps are different cell. This real predominance is in affair with the reservoir of Saleh et al. G) model m and dollUsed Banks, which seems used by collection cows, which are haunted the most early congeners that counter the chlorophyll of 201D Entomological, then narrated second functions.
Deep into Flesh aims for that classic morbid, desolate atmosphere of desiccation and decay. It is helped immensely by the sudden piano melody and the eerie opening section. The Last Glow Of Humanity is one of the more traditional cuts of the record. The slow midsection in Unchained Hatred borders treacherously close on deathcore - and slam-death territory, neither of which is advisable. Thankfully it is redeemed by a spectacular solo. Why Requiem For Ashes is its own song, and not integrated as an intro piece to A Den Of Iniquity probably has to do with that it can be used as an interlude or introduction in during live shows. Dismember and Obsessed By the Screams are the strongest cuts of the record, the former because it is the most straightforward and focused. The latter retains the focus and tight structuring and adds a number of truly exhilarating leads portions.. "There Is No Hope …Beyond Mutation" is plagued by an uneven and somewhat schizophrenic mix of American death ...
On procedure day, well apply a topical numbing cream to the treatment area for about 30 minutes. The electrical desiccation procedure itself causes minimal to no discomfort after the area has been numbed. Treatment time will vary depending on severity, but typically lasts about 30 minutes.. After treatment, some patients experience slight swelling and soreness. This can last anywhere from 2-4 days. Areas treated will form temporary scabs, but these will heal and fall off within one week to 10 days. We have the authority to prescribe oral pain medications - but most patients dont need to resort to taking these. Most patients go back to completely normal within a two week time frame. Of course, we recommend abstaining from sexual activity during the two week healing period. Typically 2-3 treatments are needed for optimal results. The procedure itself does not cause any permanent scars and there is no effect on sexual activities- so no worries!. Care to learn more about our PPP treatment? Contact ...
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TY - CHAP. T1 - Desiccation tolerance assays for acinetobacter baumannii. AU - Wang, Xun. AU - Trent, M. Stephen. AU - Davies, Bryan William. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of A. baumannii in clinical settings through contaminated healthcare equipment and personnel. Desiccation is a multifaceted stress, and many physiological and environmental factors can influence its impact on bacterial survival. This chapter provides a protocol for assessing desiccation survival that facilitates comparisons among A. baumannii strains under various environmental conditions.. AB - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important ...
Ecophysiology. Holmlund, Helen [1], Pratt, Brandon [2], Jacobsen, Anna [3], Davis, Stephen [4], Pittermann, Jarmila [1]. High-resolution computed tomography reveals dynamics of desiccation and rehydration in a desiccation-tolerant fern.. Desiccation-tolerant (DT) plants can completely desiccate to less than 10% water content (-100 MPa) and subsequently recover all function upon rehydration. These remarkable plants are aptly named "resurrection plants" for their ability to recover from apparent death. Resurrection plants often thrive in dry niches, including rocky outcrops with shallow soil. For instance, Pentagramma triangularis is a DT fern native to the semi-arid chaparral ecosystem of southern California, where it survives extended seasonal drought in the desiccated, deep dormancy state. Most DT plants are small, non-vascular plants, including bryophytes and fern gametophytes. Vascular DT plants face several additional challenges over non-vascular plants such as 1) desiccating tissues without ...
Certain organisms are able to survive almost complete loss of their internal water, entering a state of suspended animation called anhydrobiosis. When water becomes available again, the organism revives and resumes its normal activities. These organisms have been called sleeping beauties since they appear not to age during dormancy (Hengherr et al., 2008; Ricci and Covino, 2005). Desiccation tolerance is relatively widespread among prokaryotes and unicellular eukaryotes [e.g. bakers yeast, Saccharomyces cerevisiae (Potts, 1994)], but is less common in multicellular eukaryotes, being exclusive to some life stages of plants (mainly seeds and pollen), a number of resurrection plants, and some invertebrates, with well-known examples among the arthropods, tardigrades, nematodes and bdelloid rotifers (Alpert, 2006; Clegg, 2001).. In some organisms, an increase in levels of di- and oligosaccharides, especially the non-reducing sugars trehalose (in animals) and sucrose (in plants), has been ...
Mango (Mangifera indica L.) seed is recalitrant which taken from ripened fruits con,ained as high as 69.2 C75.5% moisture content (The moisture content of embryonic axis is 73.8 C86.3%). When seeds were naturally dried for 8 days, the moisture content declined to 39.1% (in embryonic axis the moisture content declined to 46.5%) and the viability of seeds completely lost. Embryonic axis lost water slower than whole seed because of the prevention of desiccation by the large cotyledons. During natural desiccation, the conductivity of leachate increased rapidly from 2.2 ̦ cm-1 g-1 (the same unit below) to 56.7, whereas the activities of dehydrogenase and acid phosphatase decreased drastically. When seeds were rapidly dried for 42 hours, the moisture content declined from 75.5 to 29.9%, the conductivity of leachate increased from 1.8 to 36.9 and the percentage germination changed from 100% to 10%. Desiccation damaged the cell membrane and decreased the activities of enzymes. Rapid drying was better ...
Salmonella can survive for long periods under extreme desiccation conditions. This stress tolerance poses a risk for food safety, but relatively little is known about the molecular and cellular regulation of this adaptation mechanism. To determine the genetic components involved in Salmonellas cellular response to desiccation, we performed a global transcriptomic analysis comparing S. enterica serovar Typhimurium cells equilibrated to low water activity (aw 0.11) and cells equilibrated to high water activity (aw 1.0). The analysis revealed that 719 genes were differentially regulated between the two conditions, of which 290 genes were up-regulated at aw 0.11. Most of these genes were involved in metabolic pathways, transporter regulation, DNA replication/repair, transcription and translation, and, more importantly, virulence genes. Among these, we decided to focus on the role of sopD and sseD. Deletion mutants were created and their ability to survive desiccation and exposure to aw 0.11 was compared to
Desiccation stress can determine the upper distribution limits and could enhance the uptake of nitrate and ammonium of intertidal algal species. Emersion following desiccation might stimulate several aspects of metabolism. Upper shore species may exhibit greater stimulation of N uptake following desiccation and achieve maximum uptake at higher desiccation levels. The objective of this study was to determine whether Porphyra species from different vertical elevations respond differently to the desiccation stress, in terms of nitrate uptake and growth. The intertidal species (Porphyra umbilicalis) and subtidal species (P. amplissima) were utilized in this study. Both species were cultivated at 100-150 umol m-2 s-1 light intensities, 500 uM nitrate concentration and 10 C (P. umbilicalis). Porphyra amplissima was cultivated at 15 C for three days, and P. umbilicalis was cultivated at 10 C for three weeks at a photoperiod of 12:12h L:D. Samples were exposed to air for 0, 30 min (30-50% water loss) ...
Desiccation stress can determine the upper distribution limits and could enhance the uptake of nitrate and ammonium of intertidal algal species. Emersion following desiccation might stimulate several aspects of metabolism. Upper shore species may exhibit greater stimulation of N uptake following desiccation and achieve maximum uptake at higher desiccation levels. The objective of this study was to determine whether Porphyra species from different vertical elevations respond differently to the desiccation stress, in terms of nitrate uptake and growth. The intertidal species (Porphyra umbilicalis) and subtidal species (P. amplissima) were utilized in this study. Both species were cultivated at 100-150 umol m-2 s-1 light intensities, 500 uM nitrate concentration and 10 C (P. umbilicalis). Porphyra amplissima was cultivated at 15 C for three days, and P. umbilicalis was cultivated at 10 C for three weeks at a photoperiod of 12:12h L:D. Samples were exposed to air for 0, 30 min (30-50% water loss) ...