2OKC: Crystal structure of Type I restriction enzyme StySJI M protein (NP_813429.1) from Bacteroides thetaiotaomicron VPI-5482 at 2.20 A resolution
Several matches in gapped BLAST to hsdS proteins and to type I site-specific deoxyribonucleases. Residues 1-200 are 34% similar to X13145, a predicted hsdS protein from Escherichia coli. Residues 6-200 are 30% similar to gb,AAD07897.1, a type I restriction enzyme S protein (hsdS) from Helicobacter pylori ...
Accepted name: type I site-specific deoxyribonuclease. Reaction: Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5-phosphates; ATP is simultaneously hydrolysed. Other name(s): type I restriction enzyme; deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent); restriction-modification system; deoxyribonuclease (adenosine triphosphate-hydrolyzing); adenosine triphosphate-dependent deoxyribonuclease; ATP-dependent DNase; type 1 site-specific deoxyribonuclease. Comments: This is a large group of enzymes which, together with those now listed as EC 3.1.21.4 (type II site-specific deoxyribonuclease) and EC 3.1.21.5 (type III site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine. They recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. They are multifunctional proteins that also ...
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Several hits in gapped BLAST to type I restriction enzyme M proteins (hsdM). E.g. 55% similarity to the experimentally documented hsdM protein (pid:450688) of E.coli. Residues 1-347 are 61% similar to the predicted hsdM protein of H.pylori (AE000596). No significant similarity to T.pallidum, C.trachomatis or M.genitalium ...
F- araD139 Δ(argF-lac)U169* rspL150 relA1 flbB5301 fruA25‡ deoC1 ptsF25 e14- This paper compares MC4100 to MG1655 and describes the significant deletions. *The paper referenced above showed that this deletion was larger than previously known. The deletion now covers ykfD-b0350. ‡The fruA25 allele is attributed to the deletion of fruB-yeiR. This means fruA is present but its promoter has been deleted. The paper also shows that the e14 element is deleted in MC4100. One of the genes removed by this deletion is mcrA, which encodes an enzyme that restricts DNA containing methylcytosine. However, other E. coli K-12 restriction/modification systems are still present in MC4100. MC4100 still encodes the McrBC 5-methylcytosine=specific restriction enzyme and the HsdR/HsdS/HsdM type I restriction-modification complex. Table three of the paper lists all genes believed to be deleted in MC4100. The methods used in the paper can detect deletions but not loss of function mutations. ...
In article ,joerg.fiedler-169D8B.09442524012001 at news.uni-ulm.de,, the eminent Jörg Fiedler at Uni Ulm wrote ,does someone know a source for is this restriction enzyme (Bst1473I) Try its iso (www.neb.com/rebase) BsaW I from NEB. Duncan -- The problem with being on the cutting edge is that you occasionally get sliced from time to time.... Duncan Clark GeneSys Ltd. Tel: +44(0)1252376288 FAX: +44(0)8701640382 http://www.dnamp.com http://www.genesys.demon.co.uk ...
ID CAPP_ECOK1 Reviewed; 883 AA. AC A1AID4; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 1. DT 22-NOV-2017, entry version 72. DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255,HAMAP-Rule:MF_00595}; DE Short=PEPC {ECO:0000255,HAMAP-Rule:MF_00595}; DE Short=PEPCase {ECO:0000255,HAMAP-Rule:MF_00595}; DE EC=4.1.1.31 {ECO:0000255,HAMAP-Rule:MF_00595}; GN Name=ppc {ECO:0000255,HAMAP-Rule:MF_00595}; GN OrderedLocusNames=Ecok1_39300; ORFNames=APECO1_2511; OS Escherichia coli O1:K1 / APEC. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=405955; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=17293413; DOI=10.1128/JB.01726-06; RA Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., RA Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., RA Nolan L.K.; RT "The genome sequence of avian pathogenic Escherichia coli strain RT O1:K1:H7 shares strong ...
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Author: Grazulis, S. et al.; Genre: Journal Article; Published in Print: 2002-02-15; Title: Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence
Read about ICONs Mapi Research Trust & Medidatas plan to develop a global library of pre-configured, pre-approved eCOA questionnaires to accelerate study start-up.
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Domain architecture and assignment details (superfamily, family, region, evalue) for gi|37676804|ref|NP_937200.1| from Vibrio vulnificus YJ016. Plus protein sequence and external database links.
DNA Restriction-Modification Enzymes: Systems consisting of two enzymes, a modification methylase and a restriction endonuclease. They are closely related in their specificity and protect the DNA of a given bacterial species. The methylase adds methyl groups to adenine or cytosine residues in the same target sequence that constitutes the restriction enzyme binding site. The methylation renders the target site resistant to restriction, thereby protecting DNA against cleavage.
Activin A Receptor Type IC兔多克隆抗体(ab111121)可与人样本反应并经IHC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
SWISS-MODEL Repository entry for A1AJP2 (RSMC_ECOK1), Ribosomal RNA small subunit methyltransferase C. Escherichia coli O1:K1 / APEC
ID M13K11RX preliminary; circular DNA; SYN; 183 BP. XX AC M22850; A01595; XX DT 15-JUN-1989 (Rel. 4, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phage vector M13K11RX - incomplete, MCS. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-183 RC from M13K11 & pXLHW7, frog H1 gene/H4 gene RA Shen W., Waye M.M.; RT "A novel method for generating a nested set of unidirectional RT deletion mutants using mixed oligodeoxynucleotides"; RL Gene 70:205-211(1988). XX RN [2] RP 1-183 RC M13K11RX RA Waye M.M.; RT ; RL Submitted (07-MAR-1989) by: RL Waye M.M., . XX RN [3] RC M13K8.1, M13K8.2, M13K8.4 from M13mp8 & oligo RC phage from M13K8.4 & M13mp11 FX RC M13K11 from phage RC M13K11RX from M13K11 RC M13K11.H4 from M13K11 & pXLHW7, histone H4 gene RA Waye M.M., Verhoeyen M.E., Jones P.J., Winter G.; RT "EcoK selection vectors for shotgun cloning into M13 and deletion RT mutagenesis"; RL Nucleic Acids Res. 13:8561-8571(1985). XX RN ...
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진행성 위암의 경우, 근치 목적의 위절제술을 받더라도 상당수의 환자에서는 암이 재발하여 결국은 환자가 사망에 이르게 된다. 재발을 줄이기 위해 수술 후 보조화학요법이 적지 않게 시도됐으나 그 치료효과를 정확히 평가할 수 있는 잘 계획된, 대규모 3상 임상시험이 없는 상황이었다. CLASSIC 연구는 이 시급한 질문에 답하기 위해 2004년에 우리 연구진이 제안했고, 로슈와 사노피 두 회사를 설득함으로써 시행됐다. 이 연구는 우리나라는 물론 중국과 타이완의 여러 병원들이 참여했고, 2005년부터 2009년까지 1,035명의 위암환자가 D2 절제술을 받은 뒤 대조군과 8회의 XCELOX 보조화학요법을 받는 군으로 무작위 배정됐다. 이 연구의 1차 endpoint는 3년 무병생존율이었고, 2012년에 XELOX 보조요법군에서 재발 risk가 44% 감소함을 보고했다 (Bang YJ et al., Lancet2012;379:315-21). ...
Here are some up to date pictures of my Build. still have a bit lift but hopefully I have it ready for the MUD with in a couple of weeks. 1992 YJ Chevy 350 700r4 trany, NP 241 T-Case 2 Body lift
Polacy żyją coraz bardziej ekologicznie. Aż 66 proc. z nas stosuje w domach przyjazne dla środowiska rozwiązania. Jak zorganizować sobie wakacje w zgodzie z naturą?
Engblom C, Pfirschke C, Zilionis R, Da Silva Martins J, Bos SA, Courties G, Rickelt S, Severe N, Baryawno N, Faget J, Savova V, Zemmour D, Kline J, Siwicki M, Garris C, Pucci F, Liao HW, Lin YJ, Newton A, Yaghi OK, Iwamoto Y, Tricot B, Wojtkiewicz GR, Nahrendorf M, Cortez-Retamozo V, Meylan E, Hynes RO, Demay M, Klein A, Bredella MA, Scadden DT, Weissleder R, Pittet MJ ...
12 małp (1995) - Jest rok 2035. Nieznany wirus niszczy ludzkość. Tylko 1% populacji uchodzi z życiem. Ludzie żyją pod ziemią. Więzień James Cole zostaje wysłany w przeszłość do roku 1996, aby zebrać...
Polacy żyją coraz bardziej ekologicznie. Aż 66 proc. z nas stosuje w domach przyjazne dla środowiska rozwiązania. Jak zorganizować sobie wakacje w zgodzie z naturą?
ID Q8PWC0_METMA Unreviewed; 498 AA. AC Q8PWC0; DT 01-OCT-2002, integrated into UniProtKB/TrEMBL. DT 01-OCT-2002, sequence version 1. DT 07-JUN-2017, entry version 83. DE SubName: Full=Type I restriction-modification system specificity subunit {ECO:0000313,EMBL:AAM31365.1}; DE EC=2.1.1.72 {ECO:0000313,EMBL:AAM31365.1}; GN OrderedLocusNames=MM_1669 {ECO:0000313,EMBL:AAM31365.1}; OS Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / OS JCM 11833 / OCM 88) (Methanosarcina frisia). OC Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; OC Methanosarcinaceae; Methanosarcina. OX NCBI_TaxID=192952 {ECO:0000313,EMBL:AAM31365.1, ECO:0000313,Proteomes:UP000000595}; RN [1] {ECO:0000313,EMBL:AAM31365.1, ECO:0000313,Proteomes:UP000000595} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88 RC {ECO:0000313,Proteomes:UP000000595}; RX PubMed=12125824; RA Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., RA ...
Duck circovirus may predispose the host to immunosuppression and may serve as an immunological trigger for further complicated disease progression. Due to the lack of a cell culture system for propagating DuCV, little is known regarding the molecular biology and pathogenesis of DuCV. The aim of this study was to describe the construction and initial in vivo characterization of full-length DNA clones of DuCV (pIC-Mu2DuCV) and its infectivity under in vivo conditions. The constructed pIC-Mu2DuCV contained two copies of the whole DuCV genome and an introduced Xho I restriction enzyme site. Eighty-one 10-day-old conventional ducklings that were free of DuCV were randomly divided equally into three groups (1, 2 and 3). The ducklings in groups 1, 2 and 3 were inoculated intramuscularly with pIC-Mu2DuCV, wild-type virus GH01 and PBS, respectively. Subsequently, all of the ducklings were examined clinically, which were each given a physical condition score, and their rectal temperatures were taken daily during
Duck circovirus may predispose the host to immunosuppression and may serve as an immunological trigger for further complicated disease progression. Due to the lack of a cell culture system for propagating DuCV, little is known regarding the molecular biology and pathogenesis of DuCV. The aim of this study was to describe the construction and initial in vivo characterization of full-length DNA clones of DuCV (pIC-Mu2DuCV) and its infectivity under in vivo conditions. The constructed pIC-Mu2DuCV contained two copies of the whole DuCV genome and an introduced Xho I restriction enzyme site. Eighty-one 10-day-old conventional ducklings that were free of DuCV were randomly divided equally into three groups (1, 2 and 3). The ducklings in groups 1, 2 and 3 were inoculated intramuscularly with pIC-Mu2DuCV, wild-type virus GH01 and PBS, respectively. Subsequently, all of the ducklings were examined clinically, which were each given a physical condition score, and their rectal temperatures were taken daily during
AMIA_STRPN (P18791 ), APPA_BACSU (P42061 ), DDPA_ECOLI (P76128 ), DPPA_ECOLI (P23847 ), DPPE_BACSU (P26906 ), GSIB_ECO57 (Q8X6V9 ), GSIB_ECOK1 (A1A968 ), GSIB_ECOL5 (Q0TJL8 ), GSIB_ECOL6 (Q8CW88 ), GSIB_ECOLI (P75797 ), GSIB_ECOUT (Q1RE95 ), GSIB_SALCH (Q57RB1 ), GSIB_SHIBS (Q323W4 ), GSIB_SHIDS (Q32IB6 ), GSIB_SHIF8 (Q0T6D2 ), GSIB_SHIFL (Q821B3 ), GSIB_SHISS (Q3Z3V3 ), HBPA_HAEIN (P33950 ), MPPA_ECOLI (P77348 ), NIKA_ECOLI (P33590 ), OPPA_BACSU (P24141 ), OPPA_ECOLI (P23843 ), OPPA_HAEIN (P71370 ), OPPA_LACLA (Q9CEK0 ), OPPA_LACLL (Q07741 ), OPPA_LACLS (Q02VA9 ), OPPA_SALTY (P06202 ), SAPA_ECOLI (Q47622 ), SAPA_HAEIN (P45285 ), SAPA_SALTY (P36634 ), SARA_STRGC (P31306 ), UF71_DEIRA (Q9RU24 ), XP55_STRLI (P06109 ), Y1030_BRUAB (Q8VQK3 ), Y1049_BRUA2 (Q2YJK2 ), Y1090_BRUSU (Q8FUX2 ), Y210_BRUME (Q8YDG6 ), Y213_HAEIN (P44572 ), Y4TO_SINFN (P55669 ...
Purified recombinant protein of Human activin A receptor, type IC (ACVR1C), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug ...
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Kacouchia NB, N Gattia KV, Kouassi-Ndjeundo J, Vroh Bi TS, Yoda M, Kouassi YM, Badou E, Ampoh YJ, Kouassi A, Buraima F, Tanon-Anoh MJ, Kouassi B. (Abidjan) ...
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The worlds leading digital cognitive assessment and eCOA platform.. CANTAB sensitively and objectively measures cognitive function, quantifies levels of performance and impairment, and demonstrates the effectiveness of interventions in brain health.. The cloud technology enables efficient, non-invasive means of assaying brain function and participant outcomes, improving clinical trials and accelerating decision-making.. ...
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Zhang YJ, Xu YF, Cook C, Gendron TF, Roettges P, Link CD, Lin WL, Tong J, Castanedes-Casey M, Ash P, Gass J, Rangachari V, Buratti E, Baralle F, Golde TE, Dickson DW, Petrucelli L. Aberrant cleavage of TDP-43 enhances aggregation and cellular toxicity ...
I was wondering why I keep seeing a smear in my bands after digestion of my vector. I digested with 1ul EcoR1 and XhoI in a 50ul reaction for 2-3 hours. I also ran the uncut vector along side it and noticed that the smear appears only after digestion. My post-doc told me to just cut the bands out and avoid the smeared parts but the fact that there is a smear after my bands bother me. Anyone know why and should I be concerned about it ...
Some adenine methyltransferases have been shown not only to protect specific DNA restriction sites from cleavage by a restriction endonuclease, but also to play a role in various bacterial processes and sometimes in bacterial virulence. This study focused on a type I restriction-modification system (designated yrmI) of Y. pseudotuberculosis. This system is composed of three adjacent genes which could potentially encode an N 6-adenine DNA methylase (YamA), an enzyme involved in site-specific recognition (YrsA) and a restriction endonuclease (YreA). Screening of 85 isolates of Y. pestis and Y. pseudotuberculosis indicated that the yrmI system has been lost by Y. pestis and that yamA (but not yrsA or yreA) is present in all Y. pseudotuberculosis strains tested, suggesting that it may be important at some stages of the epidemiological cycle of this species. To further investigate the role of yamA in Y. pseudotuberculosis survival, multiplication or virulence, a ΔyamA mutant of Y. pseudotuberculosis IP32953
Plasmid ColIb-P9 (Incll) encodes mechanisms which allow it to avoid destruction by type I and type II restriction enzymes during transfer by conjugation between strains of Escherichia coli. A genetic system was developed to analyse these mechanisms. The system relied on measuring Collb-mediated rescue of the restriction-sensitive plasmid R751 (IncPp) from destruction by EcoKI (type I) and EcoRI (type II). One Collb mechanism was known to involve a plasmid-encoded antirestriction gene known as ardA, the product of which is active against type I enzymes. Tests for alleviation of EcoKI restriction of R751, showed strong protection by a co-transferring Collb (Ard+) plasmid, slight protection when Collb was resident in the recipient and no effect when Collb was immobilised in the donor by removal of its nic site. Hence, expression of ardA is activated in the recipient cell following transfer no detectable transfer of the ArdA protein occurs from the donor to the recipient. The ardA gene is found in ...
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S be rings and assume that Rs is a finitely generated Smodule. If S is Artinian, prove that R is also. In particular, if R = Mn ( S) deduce that S is Artinian if and only if R is Artinian. 7. Suppose K ~ F are fields with dimK F = oo and let R be the subring of M2 (F) given by R = ( ~ ~)· Show first that I= (~ ~)is a minimal right ideal of R and then construct a composition series for RR· Deduce that R is right Artinian and then prove that R is not left Artinian. 8. Show that there is a one-to-one correspondence between idempotents e E EndR(V) and direct sum decompositions V = X Y. N - 1 and j = O, 1, ... , m - 1. , 26 Part I. Projective Modules since Xi+1 n YJ+1 ~ XH1 n Yj and Xi n (XH1 n YJ+1) =Xi n YJ+1· But note that the final expression for Xi,H1/Xi,j is symmetric in Xi and lj. Thus if we define Yi,j similarly by then Xi,j+1/ Xi,j equivalent. ~ Yi+i,j /Yi,j and therefore the two refinements are D A composition series for Vis a series O=Vo~Vi~···~Vn=V such that each factor Vi+1/Vi is ...
WIRB-Copernicus Group (WCG), a provider of solutions that measurably improve the quality and efficiency of clinical research, announced that industry experts Steven Herne and Michael Cioffi have joined the leadership of MedAvante-ProPhase, the Groups electronic clinical outcomes assessment (eCOA) arm. Herne and Cioffi are tasked with expanding MedAvante-ProPhases already formidable portfolio of services and technologies, which improve the quality of clinical trial data collection and analysis in the fields of CNS and mental health.. [Read More]. ...
Intercoat produce a range of cellulose based clear lacquers which are high build and fast drying. This traditional finish is typically used for less demanding applications.. Sizes available 5, 25Lt. Request HSDS, Product data sheet here.. ...
Chen WM, de Faria SM, Straliotto R, Pitard RM, Simoes-Araujo JL, Chou JH, Chou YJ, Barrios E, Prescott AR, Elliott GN, Sprent JI, Young JP, James EK ...