TY - JOUR. T1 - Effects of genetic polymorphisms in metabolic enzymes on the relationships between 8-hydroxydeoxyguanosine levels in human leukocytes and urinary 1-hydroxypyrene and 2-naphthol concentrations. AU - Kim, Yong Dae. AU - Lee, Chul Ho. AU - Nan, Hong Mei. AU - Kang, Jong Won. AU - Kim, Heon. PY - 2003/5/1. Y1 - 2003/5/1. N2 - This study was designed to investigate the relationship between environmental exposure to polycyclic aromatic hydrocarbons (PAHs) and oxidative stress, and to evaluate the effects of cigarette smoking and the genetic polymorphisms of CYP1A1, CYP2E1, GSTM1, NAT2 and UGT1A6 on the relationship. The subjects of this study were 105 healthy Korean males without occupational exposure to PAHs. The 8-hydroxydeoxyguanosine (8-OHdG) level in leukocytes, and urinary 1-hydroxypyrene (1-OHP) and 2-naphthol concentrations, were measured by high-performance liquid chromatography. Genetic polymorphisms of CYP1A1, CYP2E1, GSTM1, NAT2 and UGT1A6 were identified by PCR and ...
Deoxyguanosine kinase (ATP: deoxyguanosine 5-phosphotransferase) activity has been identified in neonatal mouse skin tissue. This activity which has a molecular weight of 44 000 was shown to be highly specific for deoxyguanosine as a substrate. Unique to deoxynucleoside kinases was the observation that this enzyme possessed a pH optimum of 5.2. In dilute solutions catalytic activity is lost, however the enzymatic activity can be stabilized by the addition of 20 micrometer MgATP or ATP. Kinetic analysis gave an apparent Km for deoxyguanosine of 7 micrometer. Double-reciprocal plots of activity vs. MgATP concentration produced a broken line with the break occurring at 0.5 mM MgATP. Below this concentration an apparent Km for MgATP of 23 micrometer was measured; above 0.5 mM MgATP an apparent Km of 265 micrometer was calculated. Mouse skin deoxyguanosine kinase was strongly inhibited by dGTP, dGDP and UDp. dGTP was a competitive inhibitor of deoxyguanisine with an apparent Ki of 1.9 micrometer. UPD (K1
2-Hexenal is an α,β-unsaturated carbonyl compound which is mutagenic, genotoxic and forms cyclic 1,N2-propanodeoxyguanosine adducts like similar propenals for which carcinogenicity was shown, e.g. acrolein or crotonaldehyde. Since humans have a permanent intake of 2-hexenal via vegetarian food this genotoxic compound is considered to play a role in human carcinogenicity. The data base is, however, presently not sufficient for a cancer risk assessment. To date no long term carcinogenicity study on 2-hexenal has been published. Detection of respective DNA adducts of this substance in animals or humans could allow cancer risk assessment. Therefore, we have developed a 32P-post-labeling technique based on nuclease P1 enrichment and TLC separation of the labeled adducts. The respective adducts are stable over a wide pH range from pH 4 to pH 11 and relatively stable against nuclease P1. The detection limit was 0.03 adducts per 106 nucleotides and the recovery was 10%. With this method we have shown ...
Cisplatin is reported to increase intracellular ROS and 8-oxodG levels (27). As Fpg and α-OGG1 overexpression has been shown to lower 8-oxodG and oxypurine-clustered DNA damage levels, and OGG1-deficient mice exhibit tissue-specific increases in 8-oxodG accumulation following ROS-inducing xenobiotic exposure (12-16, 55), we measured nuclear 8-oxodG/dG ratios in untreated and drug-treated cell clones (Fig. 5E and F). Ectopic expression of Fpg (clone fpg 6a) or α-OGG1 (clone OGG1 1d) significantly lowered endogenous nuclear 8-oxodG levels compared with the pC 1c clone control. In addition, stable transgene expression markedly inhibited an immediate increase in 8-oxodG generation following cisplatin treatment (30 μmol/L; Fig. 5E). A maximal 1.5-fold increase in 8-oxodG/dG occurred 30 minutes after treatment compared with untreated sample and was diminished by 2 hours after treatment. Oxaliplatin exposure produced a slightly lower increase in 8-oxodG/dG that also was inhibited in fpg and α-OGG1 ...
Oxidative damage of DNA has been implicated as a fundamental cause of the physiologic changes and degenerative diseases associated with aging. When DNA is impacted by oxidative stress, the chemical 8-Oxo-2′-deoxyguanosine (8-oxo-dG) is produced as a byproduct.. Becasue 8-oxo-dG is a major product of DNA oxidation, concentrations of 8-oxo-dG within a cell is a ubiquitous marker and measurement of oxidative stress. 8-oxo-dG increases with age in DNA of mammalian tissues. 1 8-oxo-dG increases in both mitochonndrial DNA and nuclear DNA with age. 2 DNA is probably the most biologically significant target of oxidative attack and may be implicated in aging, carcinogenesis and other degenerative diseases. Environmental factors, lifestyle choices such as smoking and recreational drugs, and some pharmaceuticals have also been associated with elevated urine levels of 8-oxo-dG. For example, according to multiple regression analysis, smokers excreted 50% (31-69%; 95% confidence interval) more 8-oxo-dG than ...
Anticancer therapy with cisplatin and oxaliplatin is limited by toxicity and onset of tumor resistance. Both drugs form platinum-DNA cross-linked adducts, and cisplatin causes oxidative DNA damage including the 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxodG) lesion. To assess oxidative DNA damage as a mechanism of cisplatin and oxaliplatin cytotoxicity, 8-oxodG-directed base excision repair was stably enhanced in human embryonic kidney cells by FLAG-tagged expression of human oxoguanine glycosylase 1 (α-OGG1) or its functional homologue, Escherichia coli formamidopyrimidine glycosylase (fpg). Both drugs increased reactive oxygen species and 8-oxodG levels, and cytotoxicity was decreased by antioxidant pretreatment. Ectopic expression of α-OGG1 or fpg in cell clones increased nuclear and mitochondrial 8-oxodG repair, and reduced death by reactive oxygen species initiators (H2O2, menadione) and both platinum drugs. Exposure to oxaliplatin caused a more marked and sustained block of cell ...
Evans, M.D., Cooke, M.S., Olinski, R., Gackowski, D., Rozalski, R., Siomek, A., Loft, S., Rossner, Jr. P., Sram, R., Henriksen, T., Poulsen, H.E., Weimann, A., Barbieri, A., Sabatini, L., Violante, F., Kino, S., Ochi, T., Sakai, K., Takeuchi, M., Kasai, H., Meerman, J.H.N., Halliwell, B., Jenner, A.M., Wang, H., Cerda, C., Saez, G., Haghdoost, S., Svoboda, P., Hu, C.-W., Chao, M.-R., Peng, K.-Y., Shih, W.-C., Wu, K.-Y., Orhan, H., Istanbullu, N.S., Mistry, V., Farmer, P.B., Sandhu, J., Singh, R., Cortez, C., Su, Y., Santella, R.M., Lambert, P., Smith, R. (2010). Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2′- deoxyguanosine as a noninvasive biomarker of oxidative stress. FASEB Journal 24 (4) : 1249-1260. [email protected] Repository. https://doi.org/10.1096/fj.09- ...
In the present study, we compared levels of oxidative stress using urinary levels of 15-F2t-IsoP and 8-oxodG among breast cancer cases and controls. We found significantly elevated ORs for breast cancer risk when 15-F2t-IsoP levels were categorized based on quartiles in controls (Table 1). This relationship was found even when those women who had received radiation therapy, which was found to significantly increase 15-F2t-IsoP levels, were removed from the analysis. Radiation treatment has been shown previously to result in increased levels of urinary 8-oxodG (19, 20). There was no significant relationship with 8-oxodG levels when all cases were included in the analysis. However, removal of women with radiation therapy from the analysis resulted in a significant trend of decreased risk with higher levels of urinary 8-oxodG. Although the assay variability was 25%, we still had excellent power to detect case control differences of an OR ≤0.6 or ,1.8.. Several studies have provided evidence that ...
Background: Cigarette smoking and tobacco chewing are common modes of consuming tobacco all overthe world. Parents need to be aware that germ cell integrity is vital for birth of healthy offspring as biologicalparenting begins much before birth of a child and even before conception. The present study was conducted todetermine the etiology of non-familial sporadic heritable retinoblastoma (NFSHRb), by evaluating oxidativesperm DNA damage in fathers due to use of tobacco (smoking and chewing). Materials and Methods: We recruited145 fathers of NFSHRb children and 53 fathers of healthy children (controls) in the study. Tobacco history wasobtained by personal interview. Seminal reactive oxygen species (ROS) in semen, sperm DNA fragmentationindex (DFI) and 8 hydroxy 2 deoxyguanosine (8-OHdG) levels in sperm were evaluated. The RB1 gene wasscreened in genomic blood DNA of parents of children with NFSHRb and controls. Odds ratios (ORs) derivedfrom conditional logistic regression models. Results: There was
Deoxyguanosine (dG) nucleoside molecule. DNA (deoxyribonucleic acid) building block. Atoms are represented as spheres with conventional colour coding: hydrogen (white), carbon (grey), nitrogen (blue), oxygen (red). - Stock Image F010/6822
Global Deoxyguanosine Market by Manufacturers, Countries, Type and Application, Forecast to 2022 published in Aug 2017 spread across 122 pages, providing competitive landscape analysis, market share info and more is now available for US $3480 at Market Research Reports.
The lung has been shown to be a target organ for the effects of Benzo[a]pyrene (B[a]P). 8-hydroxy-2-deoxyguanosine (oxo8dG) is a mutagenic oxidized base formed in DNA during the metabolism of B[a]P by the peroxidase and prostaglandin H synthetase systems. The objective of this study was to determine the capacity to cleave oxo8dG in the lung, liver, and kidney by measuring the activity of 8-oxogua
Chemical Names: 2-Deoxyguanosine Catalog Number: 07-4000 CAS Number: 961-07-9 Molecular Formula: 285.20 Molecular Weight: C10H13N5O4 H2O
You are viewing an interactive 3D depiction of the molecule 8-(4-amino-4-biphenylyl)-2-deoxyguanosine (C22H23N6O4) from the PQR.
[65 Pages Report] Check for Discount on N2-Isobutyryl-2-Fluoro-2-deoxyguanosine Global Market and Forecast Research report by ChemReport. DescriptionWe provide independent and unbiased information on manufacturers, prices, production...
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Administration of 1′-[2′,3′-3H]hydroxysafrole to adult female mice resulted in the formation of DNA-, ribosomal RNA-, and protein-bound adducts in the liver that reached maximum levels within 24 hr. The levels of all three macromolecule-bound adducts decreased rapidly between 1 and 3 days after injection, at which time the amounts of the DNA-bound adducts essentially plateaued at approximately 15% of the maximum level. The amounts of the protein and ribosomal RNA adducts were very low by 20 days.. Comparison by high-performance liquid chromatography of the deoxyribonucleoside adducts obtained from the hepatic DNA with those formed by reaction of deoxyguanosine and deoxyadenosine with 1′-acetoxysafrole, 1′-hydroxysafrole-2′,3′-oxide, and 1′-oxosafrole indicated that the four in vivo adducts studied were derived from an ester of 1′-hydroxysafrole. Three of the four in vivo adducts comigrated with adducts formed by reaction of 1′-acetoxysafrole with deoxyguanosine; the fourth ...
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In this study, the ELISA estimate was similar to the HPLC estimate on purified 8-OH-dG fractions from 23 urine samples. A good correlation (r = 0.833; P , 0.0001) was observed between the two estimates. It has been reported that the mean value of 8-0H-dG in human urine samples determined by HPLC-ECD (this study), HPLC-ECD (another group; Ref. 17 ), gas chromatography with mass spectrometric detection (11) , and HPLC-ELISA (this study) was 5.47 ± 2.97 (mean ± SD), 3.68-3.96, 3.33-3.95, and 5.50 ± 2.36 (mean ± SD) μg/g creatinine, respectively. These results indicate that the monoclonal antibody N45.1 used for a commercial ELISA kit is quite specific for 8-OH-dG.. However, the ELISA estimate on 120 original urine samples (9.33 ± 3.23 μg/g creatinine) was ∼2-fold higher than the HPLC estimate (4.46 ± 2.03 μg/g creatinine). A lower correlation was observed between the two estimates compared with the values on the purified 8-OH-dG fractions. Ten % of urine samples showed a high ratio of ...
Affiliation:大分大学,教育福祉科学部,准教授, Research Field:Public health/Health science,Public health/Health science,Applied health science,Applied health science, Keywords:尿中8-Hydroxydeoxyguanosine,Reactive Oxygen Metabolites,生体内酸化ストレス,尿中7-Methyleguanine,Biological Antioxygen Potentials,分子疫学,8-OH-dG,生活習慣,思春期,学校保健, # of Research Projects:5, # of Research Products:35, Ongoing Project:前駆脂肪細胞分化誘導能レベルによる思春期の健康評価と予後推定
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* found in: NADP Disodium Salt Trihydrate, DNTP Set, 2-Deoxyguanosine Monohydrate, Adenosine-5-Triphosphate (ATP), Uracil, DATP 100 mm Solution pH 7.0,..
Oxidative stress is associated with the development of various diseases including cancer, arteriosclerosis, diabetes mellitus, hypertension and metabolic syndrome. However, little is known about the involvement of 8-hydroxydeoxyguanosine (8-OHdG) dur
Research has indicated that oxidative stress is the cause of many serious diseases such as cancer, Alzheimers, arteriosclerosis and diabetes.
2-Deoxyguanosine hydrate, 99+%, ACROS Organics™ 1g; Glass bottle 2-Deoxyguanosine hydrate, 99+%, ACROS Organics™ Carbonyl Compounds
We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-78-dihydro-2-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with
There is increasing experimental evidence to indicate that O6-methyldeoxyguanosine (O6-MedG) formation in DNA is a critical cytotoxic event following exposure to certain antitumor alkylating agents and that the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (ATase) can confer resistance to these agents. We recently demonstrated a wide interindividual variation in the depletion and subsequent regeneration of ATase in peripheral blood lymphocytes of patients treated with 24-h continuous infusion of 1-p-carboxyl-3,3-dimethylphenyltriazene (CB10-277) for metastatic melanoma. We have now measured the formation of O6-MedG in the DNA of peripheral leukocytes of nine patients receiving this treatment regimen. This lesion could be detected in DNA within 1 h and a progressive increase in adduct levels occurred during the CB10-277 infusion and for 24 h after completion. Considerable interindividual variation was observed in the peak O6-MedG levels, with values ranging from 3.0 to 23.8 µmol ...
TY - JOUR. T1 - Methylation of 2′-deoxyguanosine by a free radical mechanism. AU - Crean, Conor. AU - Geacintov, Nicholas. AU - Shafirovich, Vladimir. PY - 2009/9/24. Y1 - 2009/9/24. N2 - The mechanistic aspects of the methylation of guanine in DNA initiated by methyl radicals that are derived from the metabolic oxidation of some chemical carcinogens remain poorly understood. In this work, we investigated the kinetics and the formation of methylated guanine products by two methods: (i) the combination of •CH3 radicals and guanine neutral radicals, G(-H)•, and (ii) the direct addition of •CH3 radicals to guanine bases. The simultaneous generation of •CH3 and dG(-H)• radicals was triggered by the competitive oneelectron oxidation of dimethyl sulfoxide (DMSO) and 2-deoxyguanosine (dG) by photochemically generated sulfate radicals in deoxygenated aqueous buffer solutions (pH 7.5). The photolysis of methylcob(III)alamin to form •CH3 radicals was used to investigate the direct addition ...
8-hydroxy-2-deoxyguanosine ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2-deoxyguanosine. (KA0444) - Products - Abnova
Objective: To investigate potential mechanisms of oxidative DNA damage in a rat model of type I diabetes and in murine proximal tubular epithelial cells (MCT) and primary culture of rat proximal tubular epithelial cells (RPTE).. Research Design and Methods: Phosphorylation of Akt and tuberin, 8-oxodG levels and OGG1 expression were measured in kidney cortical tissue of control and type I diabetic animals as well as in proximal tubular cells incubated with normal or high glucose.. Results: In the renal cortex of diabetic rats, the increase in Akt phosphorylation is associated with enhanced phosphorylation of tuberin, decreased OGG1 protein expression and 8-oxodG accumulation. Exposure of proximal tubular epithelial cells to high glucose (HG) causes a rapid increase in ROS generation that correlates with the increase in Akt and tuberin phosphorylation. HG also resulted in downregulation of OGG1 protein expression, paralleling its effect on Akt and tuberin. Inhibition of PI-3K/Akt significantly ...
Sigma-Aldrich offers Sigma-I6508, N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine 3′-O-succinic acid for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Our results indicate that serum 8-OHdG is increased already in prediabetes suggesting oxidative DNA damage to be present with minor elevation of blood glucose levels (BGLs). The statistically significant positive correlation between serum 8-OHdG and body mass index in the diabetic group indicates that obesity has an additive effect to increased BGL contributing to oxidative DNA damage ...
List of 10 disease causes of Intermittent high cholesterol, patient stories, diagnostic guides. Diagnostic checklist, medical tests, doctor questions, and related signs or symptoms for Intermittent high cholesterol.
The amount of work dedicated to the study of DNA oxidative damage is large enough tosuccessfully compete with the studies on lipid peroxidation (Chapters 25 and
1LAS: Structure of an oligodeoxynucleotide containing a 1,N(2)-propanodeoxyguanosine adduct positioned in a palindrome derived from the Salmonella typhimurium hisD3052 gene: Hoogsteen pairing at pH 5.2.
1LAE: Structure of the 1,N(2)-propanodeoxyguanosine adduct in a three-base DNA hairpin loop derived from a palindrome in the Salmonella typhimurium hisD3052 gene.
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
The IUPHAR/BPS Guide to Pharmacology. DG-051 ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
In this study, WANG Yanlis lab in IBP and collaborators from Netherlands demonstrated that TtAgo can independently generate and selectively load functional DNA guides. They found that the guide-free TtAgo is able to degrade unstable double-stranded DNA (dsDNA) targets, generating short DNA products, which are selectively loaded onto TtAgo and guide subsequent DNA target cleavage. They also showed that TtAgo loads dsDNA molecules with a preference toward a deoxyguanosine on the passenger strand at the position opposite to the 5′ end of the guide strand. This explains why in vivo TtAgo is preferentially loaded with guides with a 5′ end deoxycytidine. ...
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Ime (up to 72 h) and analyzed by 223488-57-1 custom synthesis HPLC-ECD and LC/MS. Figure 7 shows the representative HPLC chromatograms of TFDG incubated with
Learn more about 3-methyl-2-oxo-2-3-dihydro-1h-imidazole-4-carboxylic-acid. We enable science by offering product choice, services, process excellence and our people make it happen.
Learn more about 2-oxo-2-3-trifluoromethyl-benzyl-amino-ethyl-phenyl-amino-acetic-acid. We enable science by offering product choice, services, process excellence and our people make it happen.
TY - JOUR. T1 - Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma. AU - Huang, Chao Yuan. AU - Pu, Yeong Shiau. AU - Shiue, Horng Sheng. AU - Chen, Wei Jen. AU - Lin, Ying-Chin. AU - Hsueh, Yu Mei. PY - 2015/9/10. Y1 - 2015/9/10. N2 - Arsenic causes oxidative stress in cultured animal and human cells, and it is a well-documented human carcinogen. We conducted a hospital-based case-control study including 167 cases of urothelial carcinoma (UC) and 334 age- and gender-matched healthy controls to evaluate the relationships between urinary arsenic profiles, urinary 8-hydroxydeoxyguanosine (8-OHdG) levels, and human 8-oxoguanine DNA glycosylase (hOGG1) genotypes and UC. The urinary arsenic species were analyzed by high-performance liquid chromatography and hydride generator-atomic absorption spectrometry. Genotyping for hOGG1 (Ser326Cys) and hOGG1 (−15C,G) was performed using the ...
The recent observations that both DNA adducts and oxidative base damage are increased in the brains from Alzheimer s and Parkinson s patients and in spinal cord tissue of patients with amyotrophic lateral sclerosis (ALS) support the idea that oxidative DNA damage may contribute to the observed loss of neurons in these neurological disorders (8,9). Therefore, understanding how oxidative DNA damage is repaired (or not) is critical for understanding the pathology underlying these diseases. The overall hypothesis of this proposal is that oxidative stress induced DNA damage in neuronal cell lines leading to cellular malfunction and/or death and DNA repair enzymes are critical for the protection of neurons against oxidative stress. The following specific Aims will address this hypothesis. Specific Aim 1: Functional and biochemical characterization of the repair enzymes Ogg1, NTH, MTH, and MYH in the neuroblastoma cell lines SHSY5Y and Neuro-2A and in primary cultures of hippocampal neurons. We will ...
Epidemiological studies have shown an association between alcohol (ethanol) consumption and increased cancer risk. The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. DNA was isolated from venous blood samples obtained from 30 male non-smoking individuals before consumption of alcohol (0h) and subsequently at 3-5h following the consumption of 150mL of vodka (containing 42% pure ethanol). Additional samples were collected 24h and 48h post-alcohol consumption. The levels of N(2)-ethyl-2-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. A slight time-dependent trend showing an increase and decrease in the levels of N(2)-ethyl-dG was observed following ...
Abstract. The correlation of coronary artery disease (CAD) with pro-oxidant/antioxidant balance and oxidative DNA damage was investigated.. Seventy-seven patients with CAD and 44 healthy individuals as control were included in this study. The comparative ratios of ubiquinol-10/ubiquinone-10, 8-hydroxy-2-deoxyguanosine/deoxyguanosine and the level of MDA measured by HPLC and the activities of GPX and SOD by colorimetric approach in blood samples obtained from patients with CAD were unraveled.. 8-OHdG/dG ratios, serum MDA level and GPX activity were found significantly elevated level in serum of CAD patients compared to control group. The SOD activity was observed in stable levels in CAD patients. Ubiquinol-10/ubiquinone-10 ratio was significantly lower in patients with CAD than the controls.. The positive correlation was observed between 8-OHdG/dG ratios in both MDA levels and GPX activity, while the significant negative correlation was seemed between the ratio of 8-OHdG/dG and ubiquinol-10/ ...
Tungsten inert gas (TIG) welding represents one of the most widely used metal joining processes in industry. It has been shown to generate a large majority of particles at the nanoscale and to have low mass emission rates when compared to other types of welding. Despite evidence that TIG fume particles may produce reactive oxygen species (ROS), limited data is available for the time course changes of particle-associated oxidative stress in exposed TIG welders. Twenty non-smoking male welding apprentices were exposed to TIG welding fumes for 60 min under controlled, well-ventilated settings. Exhaled breathe condensate (EBC), blood and urine were collected before exposure, immediately after exposure, 1 h and 3 h post exposure. Volunteers participated in a control day to account for oxidative stress fluctuations due to circadian rhythm. Biological liquids were assessed for total reducing capacity, hydrogen peroxide (H2O2), malondialdehyde (MDA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) concentrations at