Periodical: Sokoloff, Louis, Martin Reivich, Charles Kennedy, M. H. Des Rosiers, C. S. Patlak, K. D. Pettigrew, O. Sakurada, and M. Shinohara. The [14C]Deoxyglucose Method for the Measurement of Local Cerebral Glucose Utilization: Theory, Procedure, and Normal Values in the Conscious and Anesthetized Albino Rat. Journal of Neurochemistry 28, 5 (1977): 897-916. Article. 20 Images ...
TY - JOUR. T1 - Cerebral glucose utilization is reduced in second test session. AU - Stapleton, June M.. AU - Morgan, Michael J.. AU - Liu, Xiang. AU - Yung, Babington C K. AU - Phillips, Robert L.. AU - Wong, Dean Foster. AU - Shaya, Elias K.. AU - Dannals, Robert F. AU - London, Edythe D.. PY - 1997/6. Y1 - 1997/6. N2 - Cerebral glucose utilization was higher during the first positron emission tomography (PET) session than during the second session, as assayed using the PET [18F]fluorodeoxyglucose method in male human volunteers. This difference was due largely to data from subjects with low trait anxiety, since subjects with high anxiety showed similar metabolism in both PET sessions. High anxiety subjects showed greater right/left ratios of cerebral metabolism than low-anxiety subjects, particularly during the second PET session. These findings suggest that the level of anxiety may be an important variable to consider in PET studies using multiple sessions.. AB - Cerebral glucose utilization ...
TY - JOUR. T1 - Ischemia-induced impairment of 2-deoxyglucose uptake and CA1 field potentials in rat hippocampal slices. T2 - Protection by 5-HT1A receptor agonists and 5-HT2 receptor antagonists. AU - Shigenobu Shibata, Shibata. AU - Yoshifumi Kagami-Ishi, Kagami-Ishi. AU - Keiko Tominaga, Tominaga. AU - Koutaroh Kodama, Kodama. AU - Showa Ueki, Ueki. AU - Shigenori Watanabe, Watanabe. PY - 1992/12/8. Y1 - 1992/12/8. N2 - Various in vitro models have been developed to study ischemia and/or hypoxia. In the present experiment, we examined whether hypoxia/hypoglycemia (ischemia) in rat hippocampal slices reduced the 2-deoxyglucose (2-DG) uptake and CA1 field potentials evoked by stimulation of Schaffer collaterals. Autoradiograms revealed that ischemia for 15 or 20 min reduced 2-DG uptake in the stratum radiatum of the CA1 and the dentate gyrus. Similarly, the CA1 field potentials of slices exposed to ischemia for 15 and 20 min decreased by about 70 and 90% after a 6-h washout. In the second ...
Positron emission tomography (PET) in use with F-18 labeled deoxyglucose (FDG) has received considerable attention in recent years for its role in studies of cerebral glucose metabolism. PET...
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2-Deoxyglucose (2-DG), a glycolytic inhibitor analog of glucose, is widely investigated in experimental and clinical oncology for targeting glucose metabolism. Inhibition of the glycolytic pathway with 2-deoxyglucose leads to sensitization of tumor cells to other apoptotic stimuli(Maschek, G., et al Cancer Res. 64: 31-34, 2004). A phase I/II clinical trial of 2-DG for the treatment of advanced solid tumors and hormone refractory prostrate cancer has been initiated. One of the objectives of this dose escalation trial was to evaluate PK of 2-DG. The drug was administered orally on a daily schedule for 2 weeks of every 3 week cycle. The standard dose escalation format was followed with a starting dose 30mg/kg, escalated to 45mg/kg and 60mg/kg. So far twelve patients have been accrued for this trial (mean age, 65.4± 9.9 yrs), and all completed the study. Repeated oral administration of 2-DG was well tolerated, with a maximum tolerated dose (MTD) not exceeding 60mg/kg consecutive-day dosing. For the ...
Combining 2-Deoxy-D-glucose with fenofibrate leads to tumor cell death mediated by simultaneous induction of energy and ER stress. Huaping Liu, Metin Kurtoglu, Clara Lucia León-Annicchiarico, Cristina Munoz-Pinedo, Julio Barredo, Guy Leclerc, Jaime Merchan, Xiongfei Liu, Theodore J. Lampidis. 2016 May 10. [Epub ahead of print]. View Article. Targeting cisplatin-resistant human tumor cells with metabolic inhibitors. Sullivan EJ, Kurtoglu M, Brenneman R, Liu H, Lampidis TJ. Cancer Chemother Pharmacol. 2013 Dec 19. [Epub ahead of print]. View Article. Increased sensitivity to glucose starvation correlates with downregulation of glycogen phosphorylase isoform PYGB in tumor cell lines resistant to 2-deoxy-D-glucose. Philips KB, Kurtoglu M, Leung HJ, Liu H, Gao N, Lehrman MA, Murray TG, Lampidis TJ. Cancer Chemother Pharmacol. 2013 Dec 1. [Epub ahead of print]. View Article. Conversion of 2-deoxyglucose-induced growth inhibition to cell death in normoxic tumor cells. Liu H, Kurtoglu M, Cao Y, Xi H, ...
Experiments were carried out on 11 anesthetized cats (Na-pentobarbital). Uptake of a glucose analogue (2-deoxy- d-glucose) by intestinal mucosa and muscularis from arterial plasma was studied in...
TY - JOUR. T1 - Effect of age and high sucrose diet on 2-deoxyglucose uptake in perfused hindlimb. AU - Eiffert, K. C.. AU - Stern, J. S.. AU - Horwitz, Barbara A. AU - Larkin, L. M.. AU - McDonald, R. B.. PY - 1993. Y1 - 1993. N2 - The purpose of this investigation was to evaluate insulin-stimulated skeletal muscle glucose uptake in male Fischer 344 rats, age 6, 12, and 27 mo, fed either a sucrose (S: 66.6% sucrose, 17.6% protein, 6.4% fat) or sucrose-free (SF: 66.6% starch, 17.6% protein, 6.4% fat) diet for 3 mo. Skeletal muscle glucose uptake (R(g)) in perfused hindlimbs was estimated from the uptake and subsequent phosphorylation of radiolabeled 2-deoxyglucose (2DG) in the gastrocnemius (GN), extensor digitorum longus (EDL), and soleus (SOL) muscles. Rat hindlimbs were perfused at a rate of 10 ml · min-1 with a modified Krebs Henseleit buffer containing bovine red blood cells (hematocrit: 40%) and 5.85 mmole · L-1 glucose along with 358 pmoles · L-1 followed by 3580 pmoles · L-1 insulin. ...
VACUETTE Glycolytic Inhibitor, Potassium Oxalate and Sodium Fluoride 4 ml, 13 x 75 mm case of 1200 for use in glucose and lactate determinations. Buy Now!
Rosen, J; Fina, J; Milholland, R; and Rosen, F, "Inhibitory effect of cortisol in vitro on 2-deoxyglucose uptake and rna and protein metabolism in lymphosarcoma p1798." (1972). Subject Strain Bibliography 1972. 218 ...
Insulin binding and 2-deoxy-d-glucose uptake were compared in the soleus, plantaris, and extensor digitorum longus (EDL) muscles, in vitro, since the known biochemical profile of the soleus differs markedly from the other two. The present study showed that insulin binding increased in all three muscles with increasing concentrations of insulin in the range of 0.2- 30 nM. However, the increase in binding of insulin to soleus at each insulin concentration exceeded that observed in the other two muscles (P , 0.05). Differences between the plantaris and EDL were not significant. The quantity of insulin bound at each concentration also increased more rapidly in the soleus than in either the plantaris (P , 0.05) or EDL (P , 0.05). Basal and insulin-stimulated uptake of 2-deoxy-d-glucose was also greater in the soleus than in the other muscles (P , 0.05). Maximal 2-deoxy-d-glucose uptake occurred at 1 nM insulin in each of the three muscles. These results indicate that in metabolically distinct types ...
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The apparent enhancement of 2-deoxy-D-glucose uptake by mouse 3T3 cells accompanying transformation by SV40 virus is not due primarily to an effect on the transport process but to enhanced phosphorylation of the sugar by intracellular kinases. Moreover, the effect is not specifically a function of the presence of the viral genome, but is a reflection of the overall growth rate and physiological state of the cell. ...
2-[2-13c]Acetamido-2-Deoxy-d-glucose/ACM478518897 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
2-Deoxy-d-glucose/ACM154178 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Product Number , 73965300. CAS Number , 154-17-6. EC , 205-823-0. Molecular Formula , C6H12O5. Molecular Weight , 164.16. Storage Temp , Harmonized Tariff code , 29400000. Signal Word , ...
Chain E. (DG)(DA)(DC)(DG)(DG)(DC)(DC)(DG)(DC)(DC)(DG)(DC)(DT)(DA)(DG)(DC)(DG). Chain F. (DC)(DG)(DC)(DT)(DA)(DG)(DC)(DG)(DT)(DG)(DC)(DG)(DG)(DC)(DC)(DG)(DT)(DC) ...
We assessed the effect of the vasodilating calcium channel blocker nitrendipine on glucose tolerance in young spontaneously hypertensive rats (SHR) (n = 15). The nitrendipine group received 1 g/kg chow for 3 weeks. Untreated SHR (n = 14) served as controls. At 3 weeks body weight was comparable, whereas systolic blood pressure was 157 +/- 9 mm Hg in nitrendipine-treated rats versus 191 +/- 10 mm Hg in controls (mean +/- SD, P , .00001). Fasting glucose was 6.8 +/- 2.7 mmol/L in nitrendipine-treated versus 8.9 +/- 1.5 mmol/L in control rats (P , .03). An intravenous glucose tolerance test (300 mg/kg) showed plasma glucose levels at 2, 5, 15, and 30 minutes to be significantly lower in the nitrendipine-treated group versus controls (two-way ANOVA, P , .03). Glucose utilization was estimated by the uptake of [3H]deoxyglucose after its intravenous administration (2 microCi/100 g body wt) to instrumented awake animals. Heart and striated muscle uptake was, respectively, 7983 +/- 5812 and 951 +/- 731 ...
Numerous laboratories have shown that hyperglycemia increases cerebral ischemic damage. This presumably results from increased lactate production and accumulation during ischemia. Although increased tissue lactic acidosis is associated with increased ischemic brain damage, this damage has not been directly linked to glycolytic flux. Because 2-deoxyglucose (2-DG) is a competitive inhibitor of glycolysis we tested its ability to reduce hyperglycemia-exacerbated ischemic brain damage. Severe forebrain ischemia was produced by the four-vessel occlusion model in rats. Four rats received 3 g/kg glucose and saline while a second group (n = 5) was injected with 3 g/kg glucose plus 1.6 g/kg 2-DG. A third group (n = 5) was treated with 1 g/kg glucose plus saline and a fourth group (n = 5) received 1 g/kg glucose and 1.6 g/kg 2-DG. All rats were injected i.p. 10 minutes prior to the ischemic insult with the same volume/kg body weight. All rats receiving the high dose of glucose alone (3 g/kg) were dead ...
We studied the uptake of 2-deoxy-D-glucose (2DG) and the synthesis of its phosphorylated product 2DG-6-phosphate (2DG-6P) by the retinas of the clawed frog (Xenopus laevis) and the bullfrog (Rana catesbeiana). Autoradiographs showed that most of the retinal 2DG uptake is by the photoreceptor layer. The 2DG accumulation by isolated Xenopus retinas was time and concentration dependent. The Kt for transport was 5.05 mM; Vmax was 6.99 X 10(-10) mol . mg-1 tissue wet weight min-1. The Km for 2DG-6P formation was estimated to be 2-3 mM and Vmax to be approximately 4 x 10(-9) mol . mg-1 min-1. 2DG uptake was inhibited competitively by glucose with a Ki of 2.29 mM. Exposure to light reduced 2DG uptake by no more than 10% as compared with dark uptake. Low sodium or ouabain (10(-4)-10(-7) M) treatment did not significantly alter 2DG uptake as compared with control retinas. In experiments upon intact, anesthetized bullfrogs, light reduced both the total amount of radioactivity acquired by the retina and ...
Our previous study demonstrated that microinjection of leptin into the ventromedial hypothalamus (VMH) dramatically increased glucose uptake in the heart, brown adipose tissue (BAT), and skeletal muscles, but not in white adipose tissue (WAT) in conscious unrestrained rats, as assessed in vivo by the 2-[3H]deoxyglucose method. Here we examined the role of the sympathetic nervous system and insulin in enhanced glucose uptake by tissues after hypothalamic leptin injection. Pretreatment with guanethidine significantly suppressed the increased glucose uptake by the tissues in response to leptin injected into the VMH, whereas bilateral adrenal demedullation had no significant effect. Treatment with propranolol but not phenoxybenzamine also decreased significantly enhanced glucose uptake by the tissues. We further examined the interaction of the effects of hypothalamic leptin and insulin administered peripherally by clamping the glucose concentrations at a constant level. When leptin was injected into ...
Recent reports using metabolism regulating drugs showed that nutrient deprivation was an efficient tool to suppress cancer progression. In addition, autophagy control is emerging to prevent cancer cell survival. Autophagy breaks down the unnecessary cytoplasmic components into anabolic units and energy sources, which are the most important sources for making the ATP that maintains homeostasis in cancer cell growth and survival. Therefore, the glucose analog 2-deoxyglucose (2DG) has been used as an anticancer reagent due to its inhibition of glycolysis. Prostate cancer cells (PC3) were treated with 2DG for 6 h or 48 h to analyze the changing of cell cycle and autophagic flux. Rapamycin and LC3B overexpressing vectors were administered to PC3 cells for autophagy induction and chloroquine and shBeclin1 plasmid were used to inhibit autophagy in PC3 cells to analyze PC3 cells growth and survival. The samples for western blotting were prepared in each culture condition to confirm the expression level of
250 µCi quantities of 2-[14C(U)]-Deoxy-D-Glucose (300-350mCi/mmol) are available for your research. Application of [14C]Deoxy-D-Glucose can be found in: glucose transporter isoform GLUT4 gene regulation and mechanisms in insulin resistance, selectively suppressing the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells, stimulatory effect of d-ephedrine on ß3-adrenoceptors in adipose tissue of rats, glucose utilization in the brain during acute seizure as a useful biomarker for the evaluation of anticonvulsants, etc. ...
250 µCi quantities of 2-[14C(U)]-Deoxy-D-Glucose (300-350mCi/mmol) are available for your research. Application of [14C]Deoxy-D-Glucose can be found in: glucose transporter isoform GLUT4 gene regulation and mechanisms in insulin resistance, selectively suppressing the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells, stimulatory effect of d-ephedrine on ß3-adrenoceptors in adipose tissue of rats, glucose utilization in the brain during acute seizure as a useful biomarker for the evaluation of anticonvulsants, etc. ...
Creative-Proteomics offer cas 66-84-2 2-amino-2-deoxy-D-[1, 2-13C2]glucose HCl (D-[1, 2-13C2]glucosamine hydrochloride). We are specialized in manufacturing Stabel Isotope Labeled Analytical Standard products.
Objective: 1, 5-anhydroglucitol (1,5-AG) is a short-term marker of metabolic control in diabetes. Its renal loss is stimulated in hyperglycemic conditions by glycosuria, which results in lowered plasma concentration. As low renal threshold for glucose has been described in HNF-1α MODY, 1,5-AG level may be altered in these patients. The purpose of this study was to assess the 1,5-AG levels in HNF-1α MODY patients and in type 2 diabetes (T2DM) subjects with a similar degree of metabolic control. In addition, we aimed to evaluate this particle as a biomarker for HNF-1α MODY.. Research Design and Methods: We included 33 diabetic patients from the Polish Nationwide Registry of MODY. In addition, we examined 43 T2DM patients and 47 non-diabetic controls. 1,5-AG concentration was measured with an enzymatic assay (GlycoMark). The ROC (receiver operation characteristic) analysis was utilized to evaluate 1,5-AG as a screening marker for HNF-1α MODY.. Results: The mean 1, 5-AG plasma concentration in ...
Aged rats (22 to 24 months) and young control rats (3 months) were tested in a battery of behavioral tests which included tests of learning, place navigation, sensorimotor integration, motor coordination, activity, and exploration. Following testing all animals were analyzed in an unanesthetized state for their local glucose utilization. Significant differences in glucose utilization were found between the aged and young groups on some behaviors and in some brain regions. There was considerable variability in the aged group in both their behavioral performance and their glucose utilization scores; thus, attempts were made to determine whether the variability in the degree of impairment within any particular behavioral test was correlated to the regional glucose utilization scores in any of the 45 brain regions analyzed. In two of the behavioral tests employed (i.e., one for learning and one for place navigation), the decline in performance correlated significantly with the decrement in regional ...
An occasion chest X-ray of a 74-year-old female revealed a thickening in her left lung, and the tumor ... PET SCAN : pathological accumulation of the radioactive marked glucose analogue at the ... PET scan (06/12/07): pathological hypercaptation of the lingula (max. SUV 6.8) 01/2008: left lingular .... ...
by Lees, J. P and Poireau, V and Tisserand, V and Grauges, E and Palano, A and Eigen, G and Brown, D. N and Kolomensky, Yu. G and Fritsch, M and Schroeder, T and Hearty, C and Mattison, T. S and McKenna, J. A and So, R. Y and Blinov, V. E and Buzykaev, A. R and zhinin, V. P and Golubev, V. B and Kravchenko, E. A and Onuchin, A. P and Serednyakov, S. I and Skovpen, Yu. I and Solodov, E. P and Todyshev, K. Yu and Lankford, A. J and Gary, J. W and Long, O and Eisner, A. M and Lockman, W. S and Vazquez, W. Panduro and Chao, D. S and Echenard, B and Flood, K. T and Hitlin, D. G and Miyashita, T. S and Ongmongkolkul, P and Rohrken, M and Huard, Z and Meadows, B. T and Pushpawela, B. G and Sokoloff, M. D and Smith, J. G and Wagner, S. R and Bernard, D and Verderi, M and Bettoni, D and Bozzi, C and Calabrese, R and Cibinetto, G and Fioravanti, E and Garzia, I and Luppi, E and Santoro, V and Calcaterra, A and de Sangro, R and Finocchiaro, G and Martellotti, S and Patteri, P and Peruzzi, I. M and Piccolo, ...
We studied regional cerebral glucose metabolism in 15 patients with a clinical diagnosis of corticobasal degeneration (CBD), 15 patients with probable Alzheimers disease (AD), and 15 healthy controls
The effect of chronic levodopa administration on the functional activity of the basal ganglia and its output regions was evaluated by means of the 2-deoxyglucose (2-DG) autoradiographic technique in rats with a unilateral 6-hydroxydopamine lesion of the nigrostriatal pathway. The rates of local cerebral glucose utilization were studied under basal conditions as well as in response to challenge with a selective D1 or D2 dopamine-receptor agonist. Levodopa (100 mg/kg/d, i.p.) was administered for 19 d either continuously via infusion with an osmotic pump or intermittently by twice-daily injections. Following a 3-d washout, glucose utilization was found to be decreased by both levodopa regimens in the nucleus accumbens; intermittent levodopa also decreased glucose utilization in the entopeduncular nucleus, subthalamic nucleus, ventrolateral thalamus, ventromedial thalamus, ventroposterolateral thalamus, and lateral habenula. In control (lesioned and treated chronically with saline) rats, the D1 ...
Cerebral metabolic rate for glucose (CMRG) was measured using the 14C-deoxyglucose technique in a stroke model of the gerbil produced by bilateral common carotid artery occlusion. During 30 minutes of ischemia, 14C-deoxyglucose uptake in the brain was increased along the border zone between the ischemic and nonischemic area and decreased in the ischemic areas. During the early stage of reperfusion (2 or 3 to 30 minutes), CMRG increased 50 to 150% in the cerebral cortex, caudoputamen and thalamus and 270 to 320% in the hippocampus, globus pallidus and amygdala. During the late stage of reperfusion (15 to 45 minutes), heterogeneity of CMRG appeared in the cerebral cortex, caudoputamen and thalamus. CMRG decreased to less than 50% of the control value in the cerebral cortex but remained at 200 to 300% of control in the hippocampus, globus pallidus and amygdala. The latter structures exhibited a larger and more protracted increase in glucose metabolism than the other structures most probably due to ...
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
4-Deoxy-4-fluoro-D-glucose 29218-07-3 MSDS report, 4-Deoxy-4-fluoro-D-glucose MSDS safety technical specifications search, 4-Deoxy-4-fluoro-D-glucose safety information specifications ect.
TY - JOUR. T1 - Tritiated 2‐deoxy‐D‐glucose. T2 - A high‐resolution marker for autoradiographic localization of brain metabolism. AU - Hammer, Ronald P.. AU - Herkenham, Miles. PY - 1984/1/1. Y1 - 1984/1/1. N2 - The technique for autoradiographic localization of 2‐deoxy‐D‐glucose (2DG) uptake has become a useful method for observing alterations of functional brain activity resulting from experimental manipulation. Autoradiographic resolution is improved using tritiated ([3H]) rather than carbon‐14 ([14C])2DG, due to the lower energy and shorter path of tritium emissions. In addition, lower 2DG uptake by white matter relative to gray matter is exaggerated in the [3H]2DG autoradiographs due to the greater absorption of tritium emissions by lipids. Using [3H]2DG, it is possible to observe differential metabolic labeling in various individual nuclei or portions of nuclei that is unresolvable using [14C]2DG in the awake, normal animal. Heterogeneous patterns of 2DG uptake seen only ...
in Neurology (2004), 63(12), 2332-2340. Objectives: To investigate whether the combination of fluoro-2-deoxy-D-glucose (FDG) PET measures with the APOE genotype would improve prediction of the conversion from mild cognitive impairment (MCI) to ... [more ▼]. Objectives: To investigate whether the combination of fluoro-2-deoxy-D-glucose (FDG) PET measures with the APOE genotype would improve prediction of the conversion from mild cognitive impairment (MCI) to Alzheimer disease ( AD). Method: After 1 year, 8 of 37 patients with MCI converted to AD (22%). Differences in baseline regional glucose metabolic rate (rCMRglc) across groups were assessed on a voxel-based basis using a two-factor analysis of variance with outcome (converters [n = 8] vs nonconverters [n = 29]) and APOE genotype (E4 carriers [E4+] [n = 16] vs noncarriers [E4-] [n = 21]) as grouping factors. Results were considered significant at p , 0.05, corrected for multiple comparisons. Results: All converters showed reduced rCMRglc in ...
Neurofunctional Imaging of Plasticity in Mouse Somatic Sensory Cortex during Development and in Maturity: The color-coded images show energy metabolism in sections cut tangentially through the right cortical hemisphere. The quantitative autoradiographic deoxyglucose method was employed for neurofunctional imaging. Outlines of the barrels in somatic sensory cortex are superimposed on the images (The nose is to the right; the midline is up). When six whiskers, that is whiskers B1-3 and D1-3, on the left side were stimulated, focal regions of increased metabolic nerve cell activity (red) were found co-localized with the corresponding barrels B1-3 and D1-3 (top image; row A is outside the plane of section; the arrow points at barrel E1). The distinct gap of low activity between the highly activated regions comprises barrels C1-3. When the follicles of whiskers C1-3 were removed on the day of birth, the barrels that would have represented these whisker did not develop and the adjacent barrels ...
The sensor was successfully deployed in all five patients and did not interfere with clinical care, blood pressure monitoring or sampling. One patient suffered a cardiopulmonary arrest; the sensor functioned successfully during resuscitation and urgent return to the operating room. One hundred and twenty reference samples ranging from 5.9 to 13.4 mmol/l were collected; 107/120 (89.2%) of GluCath measurements met ISO 15197 criteria (within ±20% of reference when BG ,4.2 mmol/l; Figure 1). In Subject 1 the sensor was inadvertently retracted into the arterial catheter during the study, leading to measurement error from arterial flush solution contamination. In a sensitivity analysis excluding this patient, 89/95 (93.7%) of measurements met ISO 15197 with a mean absolute relative difference of 9.4 ...
... molecular features. and regular cells. Curiously, inhibitors of mitochondrial breathing do not really considerably impact viability, but had been capable to boost level of sensitivity of sarcomas to inhibition of glycolysis. Additionally, inhibition of glycolysis considerably decreased intracellular ATP amounts, and level of sensitivity to 2-DG-induced development inhibition was related to respiratory prices and glycolytic addiction. Our results demonstrate book human relationships between sarcoma bioenergetics and level of sensitivity to metabolic inhibitors, and recommend that inhibition of metabolic paths in sarcomas should become additional looked into as a potential restorative technique. < 0.05, indicating that WHI-P 154 IC50 cells that are more reliant on glycolysis possess WHI-P 154 IC50 lower ATP-linked respiration rates. Level of sensitivity of human being sarcoma cells to glycolysis inhibition To examine ...
Introduction: Metabolic adaptations favor survival of cancer cells with higher glucose uptake. 2-Deoxy-glucose (2DG), a mimetic of glucose cannot undergo further glycolysis and hampers cell growth, essential during tumor metabolism. Chemotherapy with Cisplatin (Cis) and Pemetrexed (Pem) is the main treatment for malignant pleural mesothelioma (MPM). However, there is lack of evidence on the effects of 2DG in MPM.. Aim: To assess the synergistic effect of 2DG with Cis+Pem during cell migration in wound scratch assay.. Methods: Epithelioid (M14K), biphasic (MSTO) and sarcomatoid (ZL34) MPM cells were used in the study. Cells were grown into confluent monolayers and wounded. Wound widths were measured by microscopic imaging immediately and 8 hours after wound scratch in 10%FBS-RPMI (con), Cis(10μM)+Pem(200μM) or 2DG(3mM)+Cis+Pem. Statistical analyses were performed to assess cell migration.. Results: Migration with both treatments (Cis+Pem, 2DG+Cis+Pem) was significantly reduced compared to ...
Provide fine chemicals, building blocks and pharmaceutical intermediates. PI-15720 2-Deoxy-D-glucose (154-17-6) Synonym: Molecular Formula: C6H12O5 Weight: 164.16 CAS No: 154-17-6 Appearance: white crystalline solid Purity:98.0% FM Point:146-147℃ Order online from laboratory chemical supplier and distributor.
In the present study, we have shown the antitumoral effect generated by the lis/lin/GO treatment. The linamarase/linamarin system induces cell death in a proportional manner to the linamarin concentration, destroying the entire cell culture in ∼96 to 120 hours. Cyanide mainly inhibits oxidative phosphorylation, the most important cellular source of energy. However, several studies had reported that the mitochondrial function is impaired in tumor cells, resulting in an elevated rate of glycolysis (30-32), which would circumvent the described therapy. In this context, we tried to combine lis/lin system with subtoxic amounts of the glycolysis inhibitor 3-bromo-pyruvate and we did not find any improvement of the therapy (data not shown). We have nevertheless investigated a combination that increases the system aggressiveness by combining a cyanide-inducing process with a nontoxic amount of GO that accelerates death by 48 hours. In the conditions we have used, GO advances cell death without causing ...
There are two main pathways of ATP biosynthesis: glycolysis and oxidative phosphorylation. As a rule, the two pathways are not fully active in a single cell. In this review, we discuss mechanisms of glycolytic inhibition of respiration (Warburg and Crabtree effects). What are the reasons for the existence of this negative feedback? It is known that maximal activation of both processes can cause generation of reactive oxygen species. Oxidative phosphorylation is more efficient from the energy point of view, while glycolysis is safer and favors biomass synthesis. This might be the reason why quiescent cells are mainly using oxidative phosphorylation, while the quickly proliferating ones - glycolysis ...
Macdonald, H R., "Energy metabolism and t-cell-mediated cytolysis. II. Selective inhibition of cytolysis by 2-deoxy-d-glucose." (1977). Subject Strain Bibliography 1977. 1211 ...
Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesTDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 21) ...
2-Deoxy-2-Phthalimido-tri-acetofluoro-alpha-D-glucose2-Deoxy-2-Phthalimido-3,4,6-Tri-O-Acetyl-alpha-D-Glucopyranosyl Fluoride22004 1g | ...
TY - JOUR. T1 - Cerebral glucose utilization and glucose transporter expression. T2 - Response to water deprivation and restoration. AU - Koehler-Stec, Ellen M.. AU - Li, Kang. AU - Maher, Fran. AU - Vannucci, Susan J.. AU - Smith, Carolyn B.. AU - Simpson, Ian A.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - The relationship between local rates of cerebral glucose utilization (1CMR(glc)) and glucose transporter expression was examined during physiologic activation of the hypothalamoneurohypophysial system. Three days of water deprivation, which is known to activate the hypothalamoneurohypophysial system, resulted in increased ICMR(glc) and increased concentrations of GLUT1 and GLUT3 in the neurohypophysis; mRNA levels of GLUT1 and GLUT3 were decreased and increased, respectively. Water deprivation also increased 1CMR(glc) in the hypothalamic supraoptic and paraventricular nuclei; mRNA levels of GLUT1 and GLUT3 appeared to increase in these nuclei, but the changes did not achieve statistical significance. ...
An accelerated rate of glucose transport is among the most characteristic biochemical markers of cellular transformation. To study the molecular mechanism by which transporter activity is altered, cultured rodent fibroblasts transfected with activated myc, ras, or src oncogenes were used. In myc-transfected cells, the rate of 2-deoxy-D-glucose uptake was unchanged. However, in cells transfected with activated ras and src oncogenes, the rate of glucose uptake was markedly increased. The increased transport rate in ras- and src-transfected cells was paralleled by a marked increase in the amount of glucose transporter protein, as assessed by immunoblots, as well as by a markedly increased abundance of glucose transporter messenger RNA. Exposure of control cells to the tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) for 18 hours had a similar effect of increasing the rate of glucose transport and the abundance of transporter messenger RNA. For ras, src, and TPA, the ...
Brown-adipose-tissue glucose utilization rate and its insulin-sensitivity were measured in vivo in the anaesthetized rat by a 2-deoxy[1-3H]glucose technique. Glucose utilization can be increased 60-fold by insulin, to reach extremely high rates. Glucose utilization and its insulin-sensitivity are modulated in accordance with physiological or pathological conditions. ...