TY - JOUR. T1 - Correlation among dentin depth, permeability, and bond strength of adhesive resins. AU - Tagami, J.. AU - Tao, L.. AU - Pashley, D. H.. PY - 1990/1. Y1 - 1990/1. N2 - Correlations among dentin permeability, dentin depth, and dentin bonding of Scotchbond, Clearfil New Bond, and Superbond C&B were studied in bovine incisor crown segments. Since the dentin surface was prepared on deeper dentin, the permeability of the dentin increased both in the presence of, and especially after removal of, the smear layer. In general, the deeper the dentin, the lower the bond strengths of Scotchbond, Clearfil New Bond, and Superbond C&B. The higher the dentin permeability, the lower the bond strength of Superbond C&B, but there was no simple relationship in the case of either Scotchbond or Clearfil New Bond. The bond strength of Superbond C&B was much higher than those of Scotchbond or Clearfil New Bond at any depth of dentin. Superbond C&B should be considered as both an enamel- and ...
TY - JOUR. T1 - Comparative post irrigation evaluation of calcium loss and its effect on microhardness of radicular dentin. AU - Khosla, Manak. AU - Mala, Kundabala. AU - Shenoy, Ramya. PY - 2017/10/1. Y1 - 2017/10/1. N2 - This study was conducted to compare calcium loss and microhardness reduction of radicular dentin following treatment with 17% EDTA, 10% citric acid, 5% maleic acid, and MTAD, by estimating calcium loss, and radicular dentin microhardness by using Atomic absorption spectrophotometry and by Vickers hardness tester. The results were statistically evaluated using one way ANOVA test and performed at 95% level of confidence and Spearmans correlation test was applied to determine correlation between calcium liberation and radicular dentin microhardness. All the experimental chelating agents bring about calcium loss and reduction in microhardness from radicular dentin.Study concluded that at 5 minutes, 5% maleic acid as a chelating agent causes the maximum calcium loss and ...
TY - JOUR. T1 - Alternative Adhesive Strategies to Optimize Bonding to Radicular Dentin. AU - Bouillaguet, Serge. AU - Bertossa, Bruno. AU - Krejci, Ivo. AU - Wataha, John C.. AU - Tay, Franklin R.. AU - Pashley, David H.. PY - 2007/10/1. Y1 - 2007/10/1. N2 - This study tested the hypothesis that bond strengths of filling materials to radicular dentin might be optimized by using an indirect dentin bonding procedure with an acrylic core material. Roots of human teeth were endodontically prepared and obturated with EndoREZ, Epiphany, or the bonding of an acrylic point with SE Bond by using a direct or an indirect bonding technique. Bond strengths of endodontic sealers to radicular dentin were measured with a thin slice push-out test. Push-out strengths of EndoREZ and Epiphany to radicular dentin were less than 5 megapascals (MPa). The direct bonding technique with acrylic points and the self-etching adhesive had push-out strengths of 10 MPa, increasing to 18 MPa with the indirect technique. The ...
OBJECTIVES: The submicron hiatus represents a potential space between the base of the collagen network and the mineralized dentin when dentin is acid-etched for bonding. These spaces were observed in SEM studies after acid-etched dentin specimens were critical point dried or dehydrated in hexamethyldisilasane. However, they have never been identified in TEM studies of dentin hybrid layers. This study critically examined the cause of submicron hiati formation using a silver staining technique to measure nanoleakage. METHODS: Two multi-step, total-etch adhesives (One-Step, Bisco; Single Bond, 3M) and two single-step, self-etching adhesives (Prompt L-Pop, ESPE; One-Up Bond F, Tokuyama) were examined. Flat dentin surfaces were bonded with these adhesives and a lining composite. In each adhesive group, 0.8mm thick slabs from the same bonded tooth were coated with nail varnish applied 1mm from the bonded interfaces. The varnish was either left to dry completely for 10min before immersing in 50wt% ...
Purpose: To assess the effect of different peri- and intertubular dentin mineralization conditions and etching on shear bond strength in vitro. Materials and Methods: One hundred fifty crowns of extracted bovine incisors were embedded in resin and ground to expose the buccal coronal dentin. Sixty specimens were subjected to a demineralizing solution (DS) and another 60 teeth to a bacterial-based laboratory caries model (S. mutans, SM). Thirty specimens of each demineralization protocol (DS and SM) were randomly selected and remineralized (-R). Thirty sound dentin specimens served as control (C). Resin composite buildups (Tetric) were bonded after application of one of the following adhesives: a one-step self-etching adhesive (Xeno III), and a self-etching adhesive (Syntac Classic) without (three-step) and with prior additional 35% phosphoric acid etching (etch-and-rinse, four-step). Teeth were subjected to shear bond strength testing in a universal testing device at a crosshead speed of 0.5 ...
Introduction. The mechanism for adhesive bonding to dental substrates, especially to dentin, has been extensively studied in the last few decades. The establishment of effective interlocking occurs when the adhesive penetrates into the intratubular and intertubular dentin so that resin penetration into the conditioned dentin results in the formation of intratubular resin tags and a "hybrid layer."1 Micromechanical attachment is the most important mechanism of resin adhesion to dentin. However, there are several factors that can affect hybrid layer and resin tag formation, such as acid-etching, the moisture conditions of the substrate, and adhesive and dentin depth.2. During dentin acid-etching, the mineral content of the dentin surface is removed, and the collagen fibrils remain supported by water.3 The moist bonding technique prevents the collagen fibers from collapsing and provides stability to the collagen, thus enabling greater infiltration of adhesive monomers.4 However, some studies have ...
The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy
TY - JOUR. T1 - Demonstration of type III collagen in the dentin of mice. AU - Nagata, Kengo. AU - Huang, Yu Hsin. AU - Ohsaki, Yasuyoshi. AU - Kukita, Toshio. AU - Nakata, Minoru. AU - Kurisu, Kojiro. PY - 1992/1/1. Y1 - 1992/1/1. N2 - It has been reported that, although type III collagen is present in human dentin where there is dentinogenesis imperfecta and in reparative dentin, it is absent in normal dentin. In a preliminary study, however, we observed evidence showing that small amounts of fibers showing positive labeling for type III collagen are present in the molars of normal mice. In the present study, in order to localize type III in normal dentin, immunofluorescent and immunoelectron microscopic examinations of the molars of normal mice were carried out using affinity-purified antibodies to mouse type III and type I collagen. The fibers positive for type III collagen were much more frequently observed in the root than in the crown. These fibers ran in peritubular dentin or near that ...
TY - JOUR. T1 - The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells. AU - Kim, Ki Wan. AU - Yassen, Ghaeth H.. AU - Ehrlich, Ygal. AU - Spolnik, Kenneth. AU - Platt, Jeffrey A.. AU - Windsor, L. Jack. PY - 2015/10/1. Y1 - 2015/10/1. N2 - This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity ...
In this study, a fluorescence technique was developed for visualization of dentin using confocal laser scanning microscopy (CLSM). Eighteen extracted human teeth were used: 13 showing no clinical signs of caries and 5 with visually apparent decay. Preliminary study: All teeth were horizontally sectioned to approx. 200 micrometers thickness and pre-treated as follows: no pretreatment; vacuum only; ultrasonication only; sodium hypochlorite (NaOCl) only; vacuum and NaOCl; ultrasonication and NaOCl; or vacuum, ultrasonication and NaOCl. Samples were stained with Rhodamine 123 fluorescent dye at a concentration of 10-5 M in phosphate buffer saline for 1 to 24 hours. Caries study: Dentin surfaces, some with pre-existing caries, were visualized using CLSM. Most dentin tubules in sound dentin appeared open using CLSM, but most dentin tubules in carious dentin appeared closed or narrowed. Surface images obtained using CLSM were similar to those seen by SEM, but additional subsurface imaging was possible ...
Dentin is a calcified tissue of the body. Dentin, enamel, cementum and pulp, are the four major components that make up the teeth. It is often covered by enamel on the crown and cementum of the root and surrounds the tooths pulp. Dentin is comprised of 45% hydroxylapatite, 33% organic material and 22% water. Dentin is yellow in its appearance and greatly affects the tooths color because of the enamels translucency. Dentin is less mineralized and less brittle in comparison to enamel. It is also necessary for the support of the enamel. Dentin has a score of 3 on the Mohs scale of mineral hardness. Dentin forms throughout life and is very sensitive.. Dentinal sclerosis or transparent dentin-sclerosis of primary dentin is the regressive alteration in teeth caused by the calcification of the dentinal tubules. It can occur as a result of cavities, an abrasion or as part of the normal aging process. ...
The influence of dentin permeability on transdentinal LED light propagation should be evaluated since this kind of phototherapy may further be clinically used to stimulate the metabolism of pulp cells, improving the healing of damaged pulps. This study evaluated the influence of the dentin permeability on the transdentinal LED light (630 nm) transmission. Forty-five 0.5-mm-thick dentin disks were prepared from the coronal dentin of extracted sound human molars. An initial measurement of transdentinal LED light transmission was carried out by illuminating the discs in the occlusal-to-pulpal direction onto a light power sensor to determine light attenuation. The discs were treated with EDTA for smear layer removal, subjected to analysis of hydraulic conductance, and a new measurement of transdentinal LED light transmission was taken. Spearmans correlation coefficient was used for analysis of data and showed a weak correlation between dentin permeability and light attenuation (coefficient = 0.19). ...
Dentin hypersensitivity is a painful response to a non-noxious stimulus applied to exposed dentin. Two processes may expose dentin: loss of enamel and/or loss of cementum. Loss of enamel occurs by attrition associated with occlusal function, by abrasion from dietary components or incorrect toothbrushing, or by erosion associated with environmental or dietary components, particularly acids. Exposure of root dentin is also multifactorial. Periodontal disease with gingival recession, some forms of periodontal surgery, and overzealous brushing are important etiological factors that expose root dentin. In addition, in some individuals the cementum and enamel do not meet, exposing an area of dentin. The management of this condition requires a good understanding of the complexity of the problem as well as the variety of treatments available. Some authors report that lasers may provide reliable and reproducible treatment of dentin hypersensitivity. One concern for laser safety is that the heat produced ...
PURPOSE: To evaluate microtensile bond strength and interfacial nanoleakage expression of adhesive interfaces created by XP-Bond on human deproteinized dentin immediately after bonding and after 6 months of artificial aging. MATERIALS AND METHODS: Noncarious human molars were selected, middle/deep dentin substrates were exposed, and either assigned to group 1 (XP-Bond applied on collagen-depleted dentin) or to group 2 (XP-Bond applied in accordance with manufacturers instructions). In group 1, the etched dentin surface was treated with 10% NaOCl for 60 s to remove the exposed demineralized organic matrix before XP-Bond application. Composite/dentin beams were obtained in accordance with the microtensile nontrimming technique and either pulled to failure after 24 h or after 6 months artificial aging. Data were analyzed with two-way ANOVA and Tukeys post-hoc test (p , 0.05). Interfacial nanoleakage evaluation was performed on additional adhesive interfaces to quantify the amount of silver ...
Dentin (/ˈdɛntɪn/) (American English) or dentine (/ˈdɛnˌtiːn/ or /ˌdɛnˈtiːn/) (British English) (Latin: substantia eburnea) is a calcified tissue of the body and, along with enamel, cementum, and pulp, is one of the four major components of teeth. It is usually covered by enamel on the crown and cementum on the root and surrounds the entire pulp. By weight, 45% of dentin consists of the mineral hydroxylapatite, 33% is organic material, and 22% is water. Yellow in appearance, it greatly affects the color of a tooth due to the translucency of enamel. Dentin, which is less mineralized and less brittle than enamel, is necessary for the support of enamel. Dentin rates approximately 3 on the Mohs scale of mineral hardness. Dentinal sclerosis/transparent dentin-sclerosis of primary dentin is regressive alteration in tooth characterized by calcification of dentinal tubules. It can occur as a result of injury to dentin by caries or abrasion, or as part of the normal aging process. The ...
TY - JOUR. T1 - In vitro evaluation of dentin tubule occlusion for novel calcium lactate phosphate (CLP) paste. AU - Yang, Jen Chang. AU - Hu, Hsin Tai. AU - Lee, Sheng Yang. AU - Hsieh, Sung Chih. AU - Huang, Pei Chi. AU - Ma, Chen Feng. AU - Ji, Dian Yu. AU - Chang, Liang Yu. AU - Teng, Nai Chia. PY - 2017. Y1 - 2017. N2 - Introduction: The objective of this in vitro study is to evaluate the effective and long-term occlusion of dentinal tubules using a novel calcium lactate phosphate (CLP) based desensitizing agent. Methods: Dentin disks (n = 9) were pre-etched using 1 M lactic acid for 30 s and individually treated with Colgate® Pro-Relief™ paste, CLP paste, and double distilled water (ddH2O) by a rubber-cupped handpiece. Dentin disks were analyzed under optical micrographs for pre-treatment, directly after treatment, and 14 days post-treatment. One-way ANOVA and post-hoc Tukeys test were used to determine whether there were any statistically significant differences in dentinal tubule ...
PubMed journal article Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in viv were found in PRIME PubMed. Download Prime PubMed App to iPhone, iPad, or Android
The purpose of this study was to determine the uptake of fluoride by dentin after pulsed Nd:YAG laser irradiation. Under SEM examination dentin lased by pulsed Nd:YAG laser appeared to be melted; there were no changes of calcium and phosphorus levels. Dentin irradiated by pulsed Nd:YAG laser and treated with 38% Ag(NH3)2F showed greater uptake and deep penetration of fluoride than topical application of fluoride alone, SEM examination revealed that CaF2-like and Ag3PO4-like deposits were formed on the dentin surface. These results suggested that pulsed Nd:YAG laser irradition could improve the absorption of fluoride in dentin ...
Objectives: The purpose of this study was to evaluate the effects of load cycling on nanoleakage of occlusal flat surface or cervical restorations bonded with three self-etching priming systems and a single bottle system. Methods: The dentin bonding systems used in this study were Clearfil SE Bond, Unifil Bond, Prompt L-Pop and Single Bond. Flat occlusal dentin surfaces and cervical cavities from extracted human molars were bonded with one of the dentin bonding systems. After load cycling of 90N for 5000 cycles, samples were immersed in a 50% w/v solution of silver nitrate for 24h, and exposed to photodeveloping solution for 8h. Specimens were sectioned, mounted on stubs, carbon-coated and observed in a Field Emission-SEM using backscattered electron mode. One-way ANOVA was used for statistical analysis of the leakage scores. Results: FE-SEM images showed that samples subjected to load cycling had leakage patterns similar to non-load-cycled samples for all dentin bonding systems. Load cycling ...
Concentrations of calcium and phosphorus were measured in the neonatal dentine of 11 crabeater and 11 Weddell seal postcanine teeth with an energy-dispersive x-ray analyser. The extent of variation in elemental concentrations in different parts of the tooth, differences between species and individuals, and whether variation in elemental concentrations can provide information about dentine deposition mechanisms were assessed. No consistent patterns in elemental deposition in different parts of the tooth were found, but there were differences in concentrations between and within species. Post-natal dentine is composed of layers that appear alternately bright and dark in backscattered electron images. The elemental composition of neonatal dentine was closer to the dark bands than to those that appeared bright. It is suggested that the composition of neonatal dentine is more similar to the dark than the bright layers of dentine because of nutritional stresses that were occurring during mineral ...
Purpose: To examine the effects of collagenase and esterase activity on the microtensile bond strength and nanoleakage at the resin-dentin interfaces of two adhesive systems: a total-etch adhesive (Single Bond 2: SB) and a self-etch adhesive (Clearfil SE Bond: SE). Methods: Resin composites were bonded to the occlusal dentin surfaces of extracted human premolars with either SB or SE. The bonded teeth were sectioned into beams and assigned to one of four storage conditions: phosphate buffer solution (24 hours), phosphate buffer solution (4 weeks), collagenase solution (4 weeks), or esterase solution (4 weeks). Microtensile bond strength was evaluated and analyzed by two-way ANOVA. Failure mode was analyzed under SEM, and nanoleakage was examined with TEM. Results: The bond strength of SE was superior to that of SB after 4-week storage in three aqueous solutions. Collagenase and esterase solutions did not decrease the bond strength of SB any more than the phosphate buffer solution (P, 0.05). In ...
TY - JOUR. T1 - Enamel and Dentin Bond Durability of Self-Adhesive Restorative Materials. AU - Latta, Mark A.. AU - Tsujimoto, Akimasa. AU - Takamizawa, Toshiki. AU - Barkmeier, Wayne W.. PY - 2020/1/1. Y1 - 2020/1/1. N2 - PURPOSE: To use shear bond strength (SBS) and shear fatigue strength (SFS) testing to determine the durability of adhesion of self-adhesive restorative materials compared to composite resin bonded with a universal adhesive. MATERIALS AND METHODS: A universal adhesive, Prime & Bond Active, was used in self-etch mode to bond Z-100 composite resin to enamel and dentin. Three commercially available restorative materials and one experimental material with self-adhesive properties, Activa (A), Fuji II LC(F), and Equia Forte (E) and ASAR-MP4 (S) were also bonded to enamel and dentin. The SBS and SFS were determined for all materials. A staircase method was used to determine the SFS with 10 Hz frequency for 50,000 cycles or until failure occurred. RESULTS: On enamel, S generated ...
TY - JOUR. T1 - Shear bond strength of the Tenure Solution dentin bonding system.. AU - Barkmeier, Wayne W.. AU - Cooley, R. L.. PY - 1989/10. Y1 - 1989/10. N2 - A liquid solution of an oxalate bonding system containing NTG-GMA and PMDM has become commercially available. The bond strength of this oxalate adhesive (Tenure Solution) to dentin was determined by bonding composite resin cylinders to extracted teeth. The bond strengths obtained in this study are compared to the bond strengths obtained in earlier studies with the first and second generation oxalate adhesives whose components were supplied as powders and required mixing. The oxalate solutions developed significantly higher bond strengths than the original powder type systems.. AB - A liquid solution of an oxalate bonding system containing NTG-GMA and PMDM has become commercially available. The bond strength of this oxalate adhesive (Tenure Solution) to dentin was determined by bonding composite resin cylinders to extracted teeth. The ...
Objectives: To develop prototype rotary selective dentin caries excavators and to demonstrate their ability to remove only carious dentin in extracted teeth. Method and materials: Milled polymer prototype and formed wire loop prototype burs were made. They were tested on normal dentin with standardized force application and compared to carbide burs for ability to cut by weighing three extracted teeth at pre- and postcutting for each prototype version. They were tested on carious dentin of three teeth for each prototype version. The resulting excavated surfaces were analyzed with dentin caries dye, the teeth were decalcified and examined histologically, and the used prototypes were examined in light and scanning electron microscopes. Results: For both prototypes, noncarious teeth did not lose weight from prototype instrumentation, but each lost 9 or 10 mg after instrumentation with the control carbide bur. Both prototypes quickly removed carious dentin in each of the carious teeth until a ...
Objectives: This study was undertaken to examine the influence of partial demineralization of xenogenous dentin on bone formation in an osteoconductive environment. Materials and methods: Sixty dentin blocks, 2-3 mm thick and 4 mm in diameter, were prepared from developing teeth of young pigs. Forty blocks were demineralized in 24% ethylenediaminetetraacetic acid (pH 7.0) for 1, 2, 6 or 12 h. Forty adult rats divided into eight groups with five rats in each group were used. A sagittal midcranial incision was made from the occipital to the frontal region. Through a subperiostal dissection, a pocket was created on each side of the skull. One demineralized block was placed on one side, and a non-demineralized block was placed on the contralateral side, or the pocket was left empty as controls. Thus, eight experimental groups with five rats in each were formed. Results: Resorption increased significantly with increasing degree of demineralization while bone formation increased significantly with ...
Materials and Methods: Flat dentin surfaces from 50 primary molars were randomly assigned to 10 groups according to substrate (sound dentin [SD] vs caries-affected dentin [CAD] pH cycled for 14 days) and bonding approach (Scotchbond Universal Adhesive: self-etching, vs dry or wet-bonding etch-and-rinse strategies; Adper Single Bond Plus [two-step etch-and-rinse adhesive] and Clearfil SE Bond [two-step self-etching system] as controls). After 24 h of water storage, bonded sticks with cross-sectional areas of 0.8 mm2 were tested for microtensile bond strength (μTBS). Two sticks from each tooth were immersed in silver nitrate solution in order to evaluate nanoleakage (NL) with SEM. The μTBS means were analyzed using two-way ANOVA and Tukeys tests. For NL, the Kruskal-Wallis and Mann-Whitney tests were used (α = 0.05 ...
Dentine tooth tissue. Coloured scanning electron micrograph (SEM) of dentine (substantia eburnea), which is a mineralised connective tissue found under a tooths enamel. It forms the bulk of a tooth and differs from bone in its microscopic structure, which is seen here. Shown in cross-section are the dentinal tubules (dental canaliculi), which are where extensions from the odontoblast cells (part of the pulp at the core of a tooth) have formed the surrounding dentine matrix. These odontoblast extensions (or processes) allow dentine to rebuild itself, unlike enamel. Magnification: x460 when printed 10 centimetres wide. - Stock Image P486/0142
Background: To date there is not a material considered ideal for the lased dentin. Objective: To compare the bond strength to human lased dentin of self-etch and etch-and-rinse adhesive systems, a self-adhesive resin composite and a glass-ionomer cement. Methods: Forty human molars were sectioned to obtain a 2 mm-thick slab of mid-coronal dentin. The occlusal surface of each slab was polished by SiC paper (P600) for 10 s. Then an half part of dentin slabs was randomly selected for receiving treatment with 2.94 μm Er:YAG laser (DEKA, Smart 2940D Plus) with 10 Hz at 100 mJ, pulse duration of 230 μs with contact tip. Dentin slabs were randomly divided into four groups (n = 10). Six conical frustum-shaped build-ups were constructed on the occlusal surface of each dentin slab using bonding agents (OptiBond Solo Plus Group 1; OptiBond All-in-one Group 2) combined with a resin composite (Premise Flow), self-adhesive resin composites (Vertise Flow Group 3) and a glass-ionomer cement (Ketac-Fil Group 4).
Is it a valid idea that the nerve may protect itself with tertiary (new) Dentin deposits? - posted in Dental Health: There is a site which shows how peoples teeth change over time. Generally, one big age-related change is that the nerve (pulp) gets smaller. This occurs due to deposits of tertiary dentin which is the bodys defense against cavities, but also secondary dentin which is constant post-eruption dentin. http://rickwilsondmd...s-with-age.html If you scroll through tha...
Dentin is a yellow, calcified tissue, comprised of 70% mineral hydroxylapatite, 20% organic material and 10% water, produced by odontoblasts that are arranged in a layer at the pulp cavity of the tooth. Dentin is covered by enamel on the crown and cementum at the root, and affects tooth color due to the transparency of enamel. Odontoblasts send cellular projections into the mineralized dentin tissue, where they become enclosed within the channels called dentinal tubules and extend towards the outer wall of the dental pulp. Dentinal tubules also contain fluid that is thought to participate in the mechanisms responsible for sensation of pain and hypersensitivity ...
OBJECTIVE: The objective of this study was to evaluate the effect of a Biosilicate®, associated with dentin adhesive system, on microtensile bond strength (μTBS) to sound and demineralized dentin. MATERIALS AND METHODS: Eighty sound-extracted molars had their middle occlusal dentin exposed. In forty teeth, dentin was artificially demineralized (pH cycling). Sound and demineralized dentin teeth were separated into four groups (n = 10), according to the substrate treatment before restoration: Group 1-total-etching adhesive Adper TM Single bond 2 (ASB) + Biosilicate®, Group 2-ASB (without Biosilicate®), Group 3-AdheSE self-etching adhesive system (AdSE) + Biosilicate®, and Group 4-AdSE (without Biosilicate ...
Objective: To compare the fracture resistance of endodontically treated teeth with mesiodistocclusal (MOD) cavities restored with only composite resin, 3 mm glass-ionomer cement (GIC) base + composite resin, and 5 mm GIC base + composite resin. Materials and Methods: Fifty extracted intact mandibular molars were randomly divided into five groups each including 10 teeth. Group 1: No cavity preparation or endodontic treatment was applied (intact teeth). Group 2-5: Root canals were prepared with step-back technique and filled lateral condensation of gutta-percha and sealer. Group 2: No coronal restoration was achieved. Group 3: Teeth were coronally restored with only composite resin. Group 4: Coronal restorations were performed with composite resin following 3 mm GIC base placement. Group 5: Composite resin placed over 5 mm GIC base. After finishing and polishing, all specimens were kept in an incubator at 37°C in 100% humidity for 24 h and fracture resistance was tested with a Universal Testing ...
Prevest DenPro Micron Dentin Conditioner Polyacrylic Acid Cavity Conditioner Micron Dentin Conditioner is a mild polyacrylic acid solution designed to remove the dentinal smear layer and to condition dentine, thus enhancing the bond between glass ionomer
Abstract. Aims: Tensile bond is important for assessing the success of adhesive restorative materials. The aim of this study is to evaluate the tensile bond strength of resin composite restoration to Er,Cr:YSGG lased dentin bonded with three types of adhesive systems. MATERIALS AND METHODS: Occlusal enamel of eighteen molars samples was removed to expose the underlining dentine. Er,Cr:YSGG laser irradiation was performed to central dentin. Samples were assigned randomly to three groups (N = 6): Group Ι: dentin was treated total etch adhesive (Adper single bond, 3M ESPE); Group II: treated with all in one self-etch adhesive (Adper easy one, 3M ESPE); Group III: treated with two step self-etch adhesive (Clearfil SE bond, Kurary). A translucent plastic tube was fixed and filled incrementally with hybrid resin then small metal screw with ring head was embedded in last increment. Samples were stored in distilled water at 37°C for 24 hours. Tensile bond strength was measuredd using ...
Relevant peer-reviewed publications. Perdigão J, Swift EJ, Denehy GE, Wefel JS, Donly KJ (1994). In vitro bond strengths and SEM evaluation of dentin bonding systems to different dentin substrates. Journal of Dental Research 73:44-55.. Swift EJ, Hammel SA, Perdigao J, Wefel JS (1994). Prevention of root surface caries using a dentin adhesive. JADA 125:571-576.. Swift EJ, Perdigão J, Heymann HO (1995). Bonding to enamel and dentin: A brief story and state of the art, 1995.Quintessence International 26:95-110.. Perdigão J, Lambrechts P, Van Meerbeek B, Vanherle G, Lopes ALB (1995). A Field Emission SEM comparison of four post-fixation drying techniques for human dentin. Journal of Biomedical Materials Research 29:1111-1120.. Lambrechts P, Van Meerbeek B, Perdigão J, Gladys S, Braem M, Vanherle G (1996). Restorative therapy for erosive lesions. European Journal of Oral Sciences 104:229-240.. Perdigão J, Lambrechts P, Van Meerbeek B, Braem M, Yildiz E, Yücel T, Vanherle G (1996). The ...
Our long-term goal is to understand the molecular mechanisms of dentin biomineralization. The most abundant and important molecules in dentin are type I collage...
To examine the bonding state of metal-free CAD/CAM onlay restorations made from two popular resin composite blocks and a typical glass-ceramic block after cyclic loading, with and without immediate dentin sealing (IDS). Standardized mesial-distal-occlusalbuccal (MODB) cavities in 24 extracted human molars were prepared. The intra-cavity dentin surfaces of half of the cavities were immediately sealed with all-in-one adhesive and flowable composite, while those of the other half were not. All cavities were scanned, from which CAD/CAM onlays were fabricated from three types of block and cemented with an adhesive resin cement system ...
Descrição: Objetivo: Este estudo avaliou a influência de diferentes métodos de pré-tratamento da superfície dentinária sobre a resistência de união à microtração e modo de falha de um sistema adesivo de frasco único. Metodologia: Vinte e quarto terceiros molares foram incluídos em resina acrílica e desgastados... ...
To compare the polymer bur PolyBur P1 (P1) with tungsten carbide bud bur H1 SE (H1) in removing cariously altered collagen during dentin caries excavation. Fifty extracted teeth were split in the center of a carious lesion. The 100 specimens were randomly divided into 5 groups. Five dentists were asked to excavate 10 teeth each: one half with P1 and the corresponding half with H1. The time needed for caries excavation was measured. Subsequently, histological specimens were produced and analyzed by light-microscope after Mallory-Azan-staining. The thickness of remaining cariously altered collagen was measured (| 1 mm or | 1 mm). The results were statistically evaluated. The average time to excavate a cavity with P1 was 254 (± 148) sec and 202 (± 129) sec with H1. The difference in times was not statistically significant (p | 0.05). In the group P1 in 66.1% of the sections cariously altered collagen remained, whereas 33.9% showed sound collagen. In the group H1 45.7% sections had remaining cariously
To compare the polymer bur PolyBur P1 (P1) with tungsten carbide bud bur H1 SE (H1) in removing cariously altered collagen during dentin caries excavation. Fifty extracted teeth were split in the center of a carious lesion. The 100 specimens were randomly divided into 5 groups. Five dentists were asked to excavate 10 teeth each: one half with P1 and the corresponding half with H1. The time needed for caries excavation was measured. Subsequently, histological specimens were produced and analyzed by light-microscope after Mallory-Azan-staining. The thickness of remaining cariously altered collagen was measured (| 1 mm or | 1 mm). The results were statistically evaluated. The average time to excavate a cavity with P1 was 254 (± 148) sec and 202 (± 129) sec with H1. The difference in times was not statistically significant (p | 0.05). In the group P1 in 66.1% of the sections cariously altered collagen remained, whereas 33.9% showed sound collagen. In the group H1 45.7% sections had remaining cariously
90833 avhandlingar från svenska högskolor och universitet. Avhandling: Dentine caries: acid tolerant microorganisms and aspects on collagen degradation.
To evaluate the effects of intracoronal bleaching on ultimate tensile strength (UTS) of sound and etched dentine and its ultrastructure morphology. Bovine dentine specimens with (e) or without previous etching with 37% phosphoric acid for 15 s were used for the intracoronal bleaching experiments. Teeth were randomly assigned to five treatments (n = 10): (C) control - no bleaching, (SP) sodium perborate, (CP) 35% carbamide peroxide, (25% HP) 25% hydrogen peroxide and (35% HP) 35% hydrogen peroxide. Bleaching was performed four times within a 72 h interval and afterwards, dentine pulp chamber blocks were obtained. The blocks were sectioned in 0.7 mm-thick slices and these were trimmed to reduce the inner dentine to a dumbbell shape with a cross-sectional area of 0.8 mm(2). Specimens were tested with the microtensile method (0.5 mm min(-1)) and data were analysed (two-way anova-Tukey test, P , 0.05). Additional teeth were prepared for transmission electron microscopy (TEM) to evaluate dentine ...
ncbi.nlm.nih.gov. Salvatore Sauro: It seems that in this paper the authors did not rinsed the glu-primer; did they?. Renato Cilli: The glu-primer was just an aqueous solution of 5% glutaral. After application, dentin was only dried with absorbant paper in one group and in other group was additionally dried with 5-second air jet. Primer B was a solution of hema and ethanol.. Salvatore Sauro: Renato Cilli what did the author did with primer B after application? Was it rinsed or just dried ?. Renato Cilli: They applied on dentin surface primed with glutaral (or not, another group) and left to evaporate the ethanol, then applied bonding resin (scotchbond mp). Renato Cilli: Regarding the question that promoted this discussion session, yes, I guess glutaraldehyde can inhibit MMPs. But GUESS is not science, lets go to lab!. Umer Daood: Tore you nailed it. The HEMA issue within the bond. The inevitable hydrolysis of the ester bonds which than releases alcohols and further degradation. The evaluation ...
Materials and Methods: Flat mid-coronal dentin surfaces of extracted third molars (n = 28) were subjected only to grinding with a 600-grit SiC paper for 1 min (sound dentin S, n = 14) or additionally to erosive pH cycling with a cola-based soft-drink (eroded dentin E, n = 14). After acid etching, rinsing, and air drying, S and E were rehydrated with 1.5 μl of 2% CHX (S2%, n = 7; E2%, n = 7) or of distilled water (control SC, n = 7; EC, n = 7). Composite buildups were incrementally constructed with Filtek Z350 following Adper Single Bond 2 application. Specimens were sectioned into beams, which were subjected to microtensile testing immediately or after 6 or 12 months of aging. Fractured surfaces were observed under a digital microscope (50X magnification). Microtensile bond strength (μTBS) (MPa) was analyzed by three-way ANOVA and Tukeys tests (α = 0.05) and failure mode by the Kruskal-Wallis test (α = 0.05 ...
Context: Smokers are at a higher risk of contracting periodontitis. This association is established and reported in many studies. Periodontitis is a polymicrobial disease, wherein smoking is an environmental factor which aggravates the damage caused to the tissues. Procalcitonin (ProCT) is a well-known marker for inflammatory and other infectious diseases and has been assessed in this study. It has been observed that the release of reactive oxygen species (ROS) is exaggerated in smokers. Antioxidants are administered to combat the harmful effects caused by the ROS, and Oxitard™ is a powerful herbal antioxidant that can counteract the action of free radicals. Aims: As smokers are more prone to tissue injury due to excessive release of ROS, this study aimed to understand the benefits of systemic administration of Oxitard after scaling and root planing (SRP) on serum ProCT levels in smokers with chronic periodontitis (CP). Settings and Design: This was a randomized interventional study, wherein ...
Abstract. Aims: To evaluate the effect of three different adhesive systems on shear bond strength of composite resin to Er,Cr:YSGG lased dentin. Materials and methods: Twenty one sound third molars were used. Occlusal third of crowns was cut using minitom machine to expose flat dentin surface. Laser irradiation was performed on a circular test area demarcated on each dentin surface. Samples randomly assigned to three groups (n=7) according to the adhesive systems that used which were: total etch adhesive (Adper single bond, 3M ESPE), two step self etch adhesive (Clearfil SE bond, Kurary), and all in one adhesive (Adper easy one, 3M ESPE). Adhesives were applied to the lased dentin surface according to manufacture instruction. Composite rod was applied over the bonded area and cured. Samples were stored in distilled water at 370for 24 hours. The evaluation of shear bond strength was employed by the use of universal testing machine. Results: Statistical analysis of data by analysis ...
265.00. CLEARFIL LINER BOND 2V is a dual-cure user-friendly dental adhesive system with multiple indications. With the self-etching primer of CLEARFIL LINER BOND 2V, time is saved while high bond strength is obtained. The self-etching primer etches mildly and enables the collagen fibers to maintain their natural structure. The bonding liquid of Liner Bond 2V does not leave an unfilled collagen and dentin layer in the decalcificated zone, but completely fills the thin etched layer, resulting in high bond strength. The high bond strength to enamel, dentine, metal and porcelain makes CLEARFIL LINER BOND 2V a suitable adhesive system for multiple indications.. ...
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PubMed journal article Differential expression patterns of the dentin matrix proteins during mineralized tissue formatio were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
Diese Studie weist darauf hin, dass gerade im Rahmen einer regenerativen endodontischen Therapie die Anwendung von desinfizierenden Spüllösungen und Medikamenten genau bedacht werden sollte. Dennoch ist es nach verschiedenen Vorbehandlungen möglich, Wachstumsfaktoren mit einer EDTA-Konditionierung aus dem Dentin herauszulösen. Da Wachstumsfaktoren eine essenzielle Rolle bei zellulären Reaktionen spielen, könnten dentineigene Wachstumsfaktoren die Erfolgsaussichten einer Revitalisierung der Pulpa durch bessere Steuerung von zellulären Reaktionen optimieren. Möglicherweise befindet sich dazu im Dentin genau die richtige Kombination und Konzentration an bioaktiven Proteinen. Eine gezielte Freisetzung von Wachstumsfaktoren und eine Optimierung der Dentinoberfläche für Zelladhäsion und -differenzierung könnten eine bessere Vorhersehbarkeit des Therapieerfolges sowie möglicherweise auch eine Ausweitung von regenerativen endodontischen Therapiemöglichkeiten auf Zähne mit abgeschlossenem ...