The goal of our research was to prove that dental follicle stem cells are a valuable cell source in improving bone regeneration on titanium implants surfaces. The dental follicle harvesting procedure is easy to undertake since it can be performed in relation to several surgical operations, impacted tooth extraction or even less invasive tooth disclosure. The search for alternative sources of mesenchymal stem cells (such as the dental follicle) is of considerable importance because bone marrow aspiration is an invasive procedure [1] and there are significant age-related decreases in the frequency and differentiation potential of bone-marrow-derived MSCs [12]. Compared to other stem cell sources from the oral cavity, as tooth pulp, the dental follicle is a considerably larger tissue [2], is easily accessible and dental follicle stem cells have a higher proliferation capacity than stem cells from the tooth pulp [13].. One innovative aspect of this study is that the follicular sac can be harvested ...
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TY - JOUR. T1 - Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. AU - Lee, Hyo Seol. AU - Lee, Jongeun. AU - Kim, Seong Oh. AU - Song, Je Seon. AU - Lee, Jae Ho. AU - Lee, Syng Ill. AU - Jung, Han Sung. AU - Choi, Byung Jai. PY - 2013/12/23. Y1 - 2013/12/23. N2 - The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. The gene-expression profiles of the dental follicle and PDL were compared using the cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 and 125 of which were more abundant in the dental follicle and PDL, respectively. The most strongly expressed genes in the dental follicle were those related to bone development and remodeling (EGFL6, MMP8, FRZB, and NELL1), apoptosis and chemotaxis (Nox4, CXCL13, and CCL2), and tooth and embryo development (WNT2, PAX3, FGF7, AMBN, AMTN, and SLC4A4), ...
Dental stem cells, especially dental follicle cells (DFCs) as precursor cells for the periodontium have interesting prospects for regenerative dentistry. During periodontitis, butyrate as a bacterial metabolite and inflammatory agent is often found in millimolar concentrations in periodontal pockets. This study evaluates the effects of butyrate on the proliferation and osteogenic differentiation of DFCs. We assessed cell viability/proliferation (BCA assay) and osteogenic differentiation (ALP activity, alizarin staining and RT PCR) of DFCs in vitro after butyrate supplementation. Butyrate concentrations of 20 mM or higher are toxic for DFCs. At a non-toxic concentration, butyrate promotes the expression of alkaline phosphatase and collagen type-1 but inhibits the formation of calcified nodules and the induction of RUNX2 and osteocalcin under osteogenic differentiation conditions. In conclusion, DFCs are resistant to physiological high concentrations of butyrate. Butyrate facilitates the ...
MAPK is a Ser/Thr protein kinase that is widely expressed in various cells. Through a cascade reaction, the MAPK pathway transduces signals to the cell nucleus to regulate transcription, and therefore influences cell proliferation, apoptosis and differentiation (12). In the MAPK family, ERK1/2, p38 and JNK subfamilies have been extensively investigated. ERK1/2 and p38 are involved in osteogenesis-associated gene expression and bone formation in vivo (13,14). However, the full underlying mechanism of ERK1/2 and p38 pathways in osteogenic differentiation remains contradictory. Li et al (14) revealed that when the ERK1/2 and p38 signaling pathways were inhibited in dental follicle cells, osteogenic differentiation in early, middle and advanced stages was promoted. However, Xiao et al (15) reported that inhibition of ERK1/2 resulted in restrained osteoblast activity and bone formation. Therefore, ERK1/2 may exert positive or negative effects on osteogenic differentiation, which may result from ...
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The aggregate foreign shareholding through ADR/GDR/FIIs/FPIs/FDI/NRIs/PIOs in HDFC Bank Ltd. have gone below the prescribed limit stipulated under the extant FDI Policy, RBI said in a notification. The Indian rupee falls against the United States dollar, while the Asian markets trade mixed.. The BSE market breadth was skewed in favour of the bears - with 2,169 declines and 664 advances. Foreign shareholding in HDFC Bank dropped to 72 percent, thereby giving FIIs a chance to acquire a further 2 percent in the stock.. Healthcare stocks gained sharply, with shares of Sun Pharma bounced back rising over 4 per cent, on value buying and its subsidiary receiving approval for Tobramycin from the European Medicines Agency.. Among the 19 sector-specific indices of the BSE, only the FMCG index closed in the green with marginal gains of 6.69 points.. Globally, Asia and Europe traded lower after Wall Streets Trump-fuelled surge finally came to an end. Brokers said that apart from profit-booking in recent ...
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Second condition of HDFC BANK was too funny , a artist could participation by registration on web page of Art Project of HDFC BANK . after registration Artist shared his art page link with his /her contact persons , then contact persons were registered his / her contact number for vote to artist friend but that vote could not submitted to direct , that vote verification needed otp . so it was a complicated task for voters or by vote, contact persons gave their own contact number to HDFC BANK for collection of Contacts data of BNAK . it was smartness of Art project of HDFC BANK . voter were thought they voted to his / her artist friend but in reality they provided contact data to HDFC BANK . ...
Home remedies for delayed tooth eruption - What are common causes of delayed tooth eruption? Define delay. The normal range of first eruption runs from birth to 15 months. It is not a standard marker for development, nutrition or hormonal balance. Delay beyond 15 months and failure to have all deciduous teeth by 3 is often genetic. Rarely it will reflect a hormonal issue in older kids but other related symptoms would be evident at the time.
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In this paper, we demonstrate that Gα12/13 signaling can regulate different aspects of epiboly movements by two distinct mechanisms: inhibiting E-cadherin activity and modulating actin cytoskeleton organization.. Excess or reduced Gα12/13 signaling during gastrulation resulted in delayed epiboly of the deep cells and in the splitting of the df cell cluster (Fig. 1). Moreover, excess Gα12/13 activity led to the detachment of cells from embryonic tissues, which suggests that cell adhesion is defective under these circumstances (Fig. 2). All of these phenotypic characteristics resemble those observed in hab (cdh1) mutant embryos (Kane et al., 1996; Kane and Warga, 2004), which suggests a possible link between Gα12/13 signaling and E-cadherin. Indeed, although altered Gα12/13 expression did not change the expression level and cellular distribution of E-cadherin (Fig. 3), our in vivo genetic experiments demonstrated that Gα12/13 can inhibit the function of E-cadherin. In particular, we found ...
Mandibular molars can have an additional root located lingually (the radix entomolaris) or buccally (the radix paramolaris). The presence of a separate RE in the first mandibular molar is associated with certain ethnic groups. In African populations a maximum frequency of 3% is found, while in Eurasian and Indian populations the frequency is less than 5%. In populations with Mongoloid traits (such as the Chinese, Eskimo and American Indians) reports have noted that the RE occurs with a frequency that ranges from 5% to more than 30%. Because of its high frequency in these populations, the RE is considered to be a normal morphological variant (eumorphic root morphology). In Caucasians the RE is not very common and, with a maximum frequency of 3.4 to 4.2%, is considered to be an unusual or dysmorphic root morphology. The etiology behind the formation of the RE is still unclear. In dysmorphic, supernumerary roots, its formation could be related to external factors during odontogenesis, or to ...