TY - JOUR. T1 - Optimum concentration of platelet-rich fibrin lysate for human dental pulp stem cells culture medium. AU - Asri, Sandy Ratna. AU - Setiati, Hasti Dwi. AU - Asrianti, Dini. AU - Margono, Anggraini. AU - Usman, Munyati. AU - Yulianto, Indah. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Platelet Rich Fibrin Lysate (L-PRF) contains platelets, leukocytes and growth factors, all of which contribute to proliferation and healing processes. This allogeneic and autologous material can be used for cell culture supplementation. Human dental pulp stem cells (hDPSCs) were isolated from the impacted third molars of 10 healthy donors, and then cultured in 3 different supplemented media cultures (10%, 20% and 25% L-PRF). Cell proliferation was analyzed using flow citometry and MTT-Assay. Compared to cells without supplementation (control group), the L-PRF group showed significant hDPSC proliferation on day 1 (p , 0.05), with the highest proliferation rate observed in the 25% L-PRF group. Significant ...

Dental pulp stem cells (DPSCs) are stem cells present in the dental pulp, the soft living tissue within teeth. They are multipotent, so they have the potential to differentiate into a variety of cell types. Other sources of dental stem cells are the dental follicle and the developed periodontal ligament. A subpopulation of dental pulp stem cells has been described as human immature dental pulp stem cells (IDPSC). There are various studies where the importance of these cells and their regenerative capacity has been demonstrated. Through the addition of tissue-specific cytokines, differentiated cells were obtained in vitro from these cells, not only of mesenchymal linage but also of endodermal and ectodermal linage. Among them are the IPS, MAPCs cells.[citation needed] Several publications have stressed the importance of the expression of pluripotentiality associated markers: the transcription factors Nanog, SOX2, Oct3/4, SSEA4, CD13, are indispensable for the stem cells to divide indefinitely ...

Objectives The aim of this study was to evaluate the level of odontogenic differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM. Methods DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and mineral deposition was observed by Von Kossa

Background. The goal of treating exposed pulp with an appropriate pulp capping material is to promote the dentinogenic potential of the pulpal cells. There have been recent attempts to develop more effective pulp-capping materials. Objectives. The aim of this study was to evaluate the effect of newly developed calcium silicate-based material on odontogenic differentiation of primary human dental pulp cells (HDPCs), in comparison with a contemporary calcium silicate-based material. Material and methods. Human dental pulp cells isolated from dental pulps were cultured in standard culture conditions in Dulbeccos Modified Eagles Medium (DMEM) and then the effects of Micro-Mega mineral trioxide aggregate (MM-MTA) (Micro-Mega, Besancon, France) and ProRoot MTA (MTA) (Dentsply Sirona, Tulsa, USA) (positive control) were evaluated on HDPCs at 1, 7 and 14 days. Untreated cells were used as a negative control. Odontoblastic differentiation was assessed by alkaline phosphatase (ALP) activity. Runtrelated ...

Stem cells derived from dental tissues—dental stem cells—are favored due to their easy acquisition. Among them, dental pulp stem cells (DPSCs) extracted from the dental pulp have many advantages, such as high proliferation and a highly purified population. Although their ability for neurogenic differentiation has been highlighted and neurogenic differentiation using electrospun nanofibers (NFs) has been performed, graphene-incorporated NFs have never been applied for DPSC neurogenic differentiation. Here, reduced graphene oxide (RGO)-polycaprolactone (PCL) hybrid electrospun NFs were developed and applied for enhanced neurogenesis of DPSCs. First, RGO-PCL NFs were fabricated by electrospinning with incorporation of RGO and alignments, and their chemical and morphological characteristics were evaluated. Furthermore, in vitro NF properties, such as influence on the cellular alignments and cell viability of DPSCs, were also analyzed. The influences of NFs on DPSCs neurogenesis were also

Human dental pulp stem cells cryopreserved at low passage (P2). Dental pulp stem cells can be differentiated to multiple cell types.

Flavonols are a kind of flavonoids which are the pigments of plants. Flavonols have lots of biological activities including antioxidant, anti-inflammatory, promoting osteogenesis, anti-osteoclastogenesis, etc. It was known that stem cells played an important role in osteogenesis. Stem cells have great potential in wound healing due to their self-renewal and multilineage differentiation potential. In our previous study, we had found the particular flavonols with osteogenic activitity for human dental pulp stem cells (DPSC). The osteogenic activity of flavonols may be helpful in alveolar bone regeneration clinically. This study is devoted to clearly understand the molecular mechanism of flavonol-induced osteogenesis in DPSCs. These include the effects of these compounds on cell physiology, intracellular signal transduction, the regulation of osteogenesis-related genes/proteins and also the calcium deposition. This study is beneficial in drug development for DPSC-based bone regeneration ...

The dental pulp stem cell industry is a rapidly evolving industry. Every month there are major events occurring in the sector that shift industry dynamics. Often, these events are announcements of technical or scientific advancements. Sometimes they are announcements of major industry alliances. Occasionally, they are announcements of a new industry competitor, a major milestone, or a significant funding award.. For those of us interested in the dental pulp stem cell industry, tracking these shifting industry dynamics is of paramount importance. For this reason, this post covers the most significant neural stem cell industry news events as of July 2015.. [Read more…] ...

Maintaining or regenerating a vital pulp is a preferable goal in current endodontic research. In this study, human dental pulp cell aggregates (spheres) were applied onto bovine and human root canal models to evaluate their potential use as pre-differentiated tissue units for dental pulp tissue regeneration. Human dental pulp cells (DPC) were derived from wisdom teeth, cultivated into three-dimensional cell spheres and seeded onto bovine and into human root canals. Sphere formation, tissue-like and mineralization properties as well as growth behavior of cells on dentin structure were evaluated by light microscopy (LM), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Spheres and outgrown cells showed tissue-like properties, the ability to merge with other cell spheres and extra cellular matrix formation; CLSM investigation revealed a dense network of actin and focal adhesion contacts (FAC) inside the spheres and a pronounced

TY - JOUR. T1 - Immunohistochemical demonstration of lymphatic vessels in human dental pulp. AU - Sawa, Yoshihiko. AU - Yoshida, Shigemitsu. AU - Ashikaga, Yuichi. AU - Kim, Takenori. AU - Yamaoka, Yuji. AU - Suzuki, Masatsugu. PY - 1998. Y1 - 1998. N2 - The existence of lymphatic vessels in dental pulp has been a matter of continuing controversy because of the difficulty of discriminating them in ordinary stained tissue sections. Recently, we have succeeded in establishing a new identification method for lymphatic vessels in human frozen sections by using a commercial monoclonal antibody specific for the human thoracic duct and anti-human laminin antiserum. The present study aimed to examine the lymphatic vessels in human dental pulp using the new immunostaining method, and compared the results with those in human small intestine. The study clearly demonstrated the distribution of lymphatic vessels in human dental pulp. Large lymphatic vessels are located in the central part of the pulp and ...

Elevated osteogenic potential of stem cells from inflammatory dental pulp tissues by Wnt4 overexpression for treating bone defect in rats

Background: the main objective is to evaluate the way to graft the dental pulp stem cells (DPSC) in periodontal defects that best regenerate periodontal tissues. Numerous procedures have been done to promote periodontal regeneration. Bone grafts show good gains clinically and radiographically but histologically seem to have minimal osteoinductive capacity. Another option that exceeds conventional surgery in reducing probing depth and increasing insertion is guided tissue regeneration and tissue engineering that could be an alternative approach to help in the regeneration of living functional bone and peri-dental structures. Material and Methods: a search was carried out in Cochrane, PubMed-MEDLINE and Scopus databases with keywords: dental pulp stem cells, periodontal regeneration, guided tissue regeneration, periodontal, tissue regeneration, periodontal bone defects, periodontal tissue engineering and periodontal defect. Inclusion criteria were articles in English, maximum 10 ...

Isolation of SHEDs and DPSCs, and cell culture. Human SHEDs and DPSCs were isolated as described previously (16, 17). Briefly, exfoliated deciduous teeth (from individuals 6-12 years old) and adult third molars (18-30 years old) extracted for clinical purposes were collected. After separation of the crown and root, the dental pulp was isolated and then digested in a solution of 3 mg/ml collagenase type I and 4 mg/ml dispase for 1 hour at 37°C. Single-cell suspensions (1 × 104 to 2 × 104 cells/ml) were plated on culture dishes in DMEM supplemented with 10% fetal calf serum, then incubated at 37°C in 5% CO2. Mesenchymal stem cells of three human bone marrow lines (hBMSCs, from individuals 20-22 years old) at passage 5 and three human skin-fibroblast lines (hFbs, 36-40-years old) at passage 5 were obtained from Lonza and the Health Science Research Resources Bank Japan, respectively. Real-time PCR and microarray analysis. Total RNA was quantified by a spectrophotometer, and RNA integrity was ...

Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. Maximal production of the IL-10R protein in dental pulp fibroblast cultures was detected by Western blot analysis after 12 h of LPS stimulation. In contrast, the human skin fibroblast (SF-MA) and human monocyte (U937) cell lines expressed IL-10R mRNA. Anti-CD14 antibodies inhibited P. intermedia LPS-induced IL-10R mRNA expression. These results indicate that P. intermedia LPS induces IL-10R gene expression in human, dental pulp fibroblasts in vitro ...

Experiments have previously demonstrated the therapeutic potential of mobilized dental pulp stem cells (MDPSCs) for complete pulp regeneration. The aim of the present pilot clinical study is to assess the safety, potential efficacy, and feasibility of autologous transplantation of MDPSCs in pulpectomized teeth. Five patients with irreversible pulpitis were enrolled and monitored for up to 24 weeks following MDPSC transplantation. The MDPSCs were isolated from discarded teeth and expanded based on good manufacturing practice (GMP). The quality of the MDPSCs at passages 9 or 10 was ascertained by karyotype analyses. The MDPSCs were transplanted with granulocyte colony-stimulating factor (G-CSF) in atelocollagen into pulpectomized teeth. The clinical and laboratory evaluations demonstrated no adverse events or toxicity. The electric pulp test (EPT) of the pulp at 4 weeks demonstrated a robust positive response. The signal intensity of magnetic resonance imaging (MRI) of the regenerated tissue in the root

TY - JOUR. T1 - The effects of etching enamel with acid on human dental pulp. A preliminary study. AU - Lipke, Edward. AU - Mautner, Richard. AU - Browdy, David. AU - Rosenberg, Paul. PY - 1979. Y1 - 1979. N2 - Enamel acid-etching has become a widespread, popular clinical procedure in recent years. Numerous studies have investigated the nature of the acid-treated enamel. However, little or no information is presently available pertaining to the effects of acid treatment upon dental pulp tissue. A limited investigation was carried out on bilateral orthodontic extraction cases to determine the effects of enamel acid-etching upon healthy dental pulp. In each of five cases one premolar served as the experimental tooth, while the contralateral premolar served as the control. After a 24-hour postoperative interval there appeared to be no basic histologic difference between the experimental and control groups.. AB - Enamel acid-etching has become a widespread, popular clinical procedure in recent ...

Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of beta-glycerophosphate (beta-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time-and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the beta-GP concentration, and there was also an influence on MEPE expression when different concentrations of beta-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, ...

Kishimoto N, Asakawa K, Madelaine R, Blader P, Kawakami K, Sawamoto K (2013) Interhemispheric asymmetry of olfactory input-dependent neuronal specification in the adult brain. Nat Neurosci 16: 884-888. Pubmed. Yamagata M, Yamamoto A, Kako E, Kaneko N, Matsubara K, Sakai K, Sawamoto K, Ueda M (2013) Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice. Stroke 44: 551-554. Pubmed. 2012. Kato Y, Kaneko N, Sawada M, Ito K, Arakawa S, Murakami S, Sawamoto K (2012) A subtype-specific critical period for neurogenesis in the postnatal development of mouse olfactory glomeruli. PLoS ONE 7 (11): e48431. doi: 10.1371/journal.pone.0048431. FREE Full text Nakaguchi K, Jinnou H, Kaneko N, Sawada M, Hikita T, Saitoh S, Tabata Y, Sawamoto K (2012) Growth factors released from gelatin hydrogel microspheres increase new neurons in the adult mouse brain. Stem Cells Int 2012, Article ID 915160, 7 pages, doi: 10.1155/2012/915160. FREE Full Text. Kako E, Kaneko N, Aoyama ...

Human adult dental pulp stem cells enhance poststroke functional recovery through non-neural replacement mechanisms. - Wai Khay Leong, Tanya L Henshall, Agnes Arthur, Karlea L Kremer, Martin D Lewis, Stephen C Helps, John Field, Monica A Hamilton-Bruce, Scott Warming, Jim Manavis, Robert Vink, Stan Gronthos, Simon A Koblar

Damage or exposure of the dental pulp requires immediate therapeutic intervention. This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24 h after stimulation. In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU

Adult stem cells are an inexhaustible source of multipotent cells, which can differentiate into various cell lines. Thanks to these properties, adult stem cells can be used in regenerative medicine for various purposes. Dental pulp stem cells (DPSC) can give rise to different cells of mesenchymal origin, such as osteoblasts, adipocytes, chondrocytes and muscle cells. At present, DPSC applications are mainly focused on bone regeneration in dental/maxilla-facial and orthopedic surgery. Current methods for preserving and banking dental pulp stem cells include steps in which DPSC are recovered from tooth, amplified and then stored in liquid nitrogen for further application. The Italian medical research centre has developed a simpler and inexpensive method for cryopreserving a tooth with its dental pulp stem cells. Tooth laser perforation allow to access the root canal without overheating the dental pulp; this step contributes to ensure the integrity of the cryopreserved dental tissue, absence of ...

The odontoblasts of dental pulp tissue form primary and secondary dentine. If the pulp tissue is exposed, the odontoblast layer will be destroyed. The aim of direct pulp capping is to induce new hard tissue formation in order to keep the pulp tissue vital. If direct pulp capping is successful, tertiary dentine is formed. This hard tissue is denoted as reparative dentine and defined as a dentine matrix, which is formed by a new generation of odontoblast-like cells after an appropriate stimulus. Thus, reparative dentine is not formed by the original post-mitotic odontoblasts. Until recently it has been unclear which pulp cells differentiate into these odontoblast-like cells. Besides multipotent adult stem cells, fibroblasts and un-differentiated mesenchymal cells are mentioned in the literature as progenitor cells for odontoblast-like cells, because their cell-division rate increases significantly after direct pulp capping. The increase in the cell division rate of fibroblasts can be explained by ...

During normal pulp tissue healing, inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or interleukins, act in the initial 48 hours (inflammatory phase) and play important roles not only as chemo-attractants of inflammatory cells and stem/progenitor cells but also in inducing a cascade of reactions toward tissue regeneration or reparative dentin formation or both. Previous reports have shown that inflammatory cytokines regulate the differentiation capacity of dental pulp stem/progenitor cells (DPCs), but none has interrogated the impact of these cytokines on the stem cell phenotype of stem/progenitor cells. This study investigated the effects of a short-term treatment with TNF-α on the stem cell phenotype and differentiation ability of human DPCs. An in vivo mouse model of pulp exposure was performed for analysis of expression of the mesenchymal stem cell marker CD146 in DPCs during the initial stage of inflammatory response. For in vitro studies, human DPCs were isolated and incubated

A group of Japanese researchers from the Nippon Dental University in Tokyo has conducted an experiment in which they have prepared liver cells from human dental pulp.. The research, which has been published in the Journal of Breath Research, has revealed that hydrogen sulphide (H2S), a major cause of bad breath, was the main component in transforming the cells into liver cells.. Lead author Ken Yaegaki said that they collected the pulp from the people who were coming in routine for dental checkups. He further affirmed that it is one of its kind research in which liver cells have been made from human dental pulp. The best part of the research is that the liver cells have been produced of great quality, which means there were fewer wrong cells.. High purity means there are less wrong cells that are being differentiated to other tissues, or remaining as stem cells. Moreover, these facts suggest that patients undergoing transplantation with the hepatic (liver) cells may have almost no possibility ...

Despite tremendous efforts to develop stem cell-based tooth regenerative strategies, the identification of intrinsic properties of pulpal stem cells is an unresolved question. Dental pulp stem cells are heterogeneous cell subpopulations likely composed of stem cells at different differentiation stages along odontoblastic lineage, which has broken their characterization. Another major drawback in the dental field is that the physiological signals governing the recruitment of pulpal stem cells for tooth repair upon injury still remain unknown.. A decade ago, our laboratory established clonal odontogenic cell lines with stem cell properties from first molar tooth germs of day 18 mouse embryos transgenic for an adenovirus-SV40 recombinant plasmid (pK4) (Priam et al. 2005). These dental pulp-derived cell lines are stable and maintain an undifferentiated phenotype under long-term standard culture conditions. Among the pulp-derived clones, the A4 cell line behaves as a multipotent mesoblastic stem ...

The dental pulp is a common source of pain and is used to study peripheral inflammatory pain mechanisms. Results show most fibers are unmyelinated, yet recent findings in experimental animals suggest many pulpal afferents originate from fibers that are myelinated at more proximal locations. Here we use the human dental pulp and confocal microscopy to examine the staining relationships of neurofilament heavy (NFH), a protein commonly expressed in myelinated afferents, with other markers to test the possibility that unmyelinated pulpal afferents originate from myelinated axons. Other staining relationships studied included myelin basic protein (MBP), protein gene product (PGP) 9.5 to identify all nerve fibers, tyrosine hydroxylase (TH) to identify sympathetic fibers, contactin-associated protein (caspr) to identify nodal sites, S-100 to identify Schwann cells and sodium channels (NaChs). Results show NFH expression in most PGP9.5 fibers except those with TH and include the broad expression of NFH in axons

Pathological stimuli, such as bacterial activity, dental bleaching, and nonpolymerized resin monomers, can cause death of dental pulp cells (DPCs) through oxidative stress- (OS-) induced mitochondrial dysfunction. However, the crucial molecular mechanisms that mediate such a phenomenon remain largely unknown. OS is characterized by the overproduction of reactive oxygen species (ROS), e.g., H|sub|2|/sub|O|sub|2|/sub|, O|sub|2|/sub||sup|−|/sup|, and |sup|⋅|/sup|OH. Mitochondria are a major source of ROS and the principal attack target of ROS. Cyclophilin D (CypD), as the only crucial protein for mitochondrial permeability transition pore (mPTP) induction, facilitates the opening of mPTP and causes mitochondrial dysfunction, leading to cell death. In the present study, we hypothesized that CypD-mediated mitochondrial molecular pathways were closely involved in the process of OS-induced death of human DPCs (HDPCs). We tested the phenotypic and molecular changes of HDPCs in a well-established

Durham, NC - In a new study published in STEM CELLS Translational Medicine, researchers used a type of platelet-derived growth factor called PDGF-BB that enhances cells ability to regenerate dentin-pulp complex.. Many in the medical community view stem cell therapy as a promising new strategy for repairing teeth once thought to be irreversibly damaged by tooth decay or dental injuries. The benefits of human dental pulp stem cells (hDPSCs), isolated from the living connective tissue in the tooths center, on such damage have been well documented in studies.. However, previous studies also revealed several problems with this type of treatment, including limitations on how much mineralized tissue can be formed when scaffolds with hDPSCs alone were implanted in nude mice. More importantly, the narrow root canal of a tooth limits tissue infiltration and the revascularization process, which also worked against the implanted hDPSCs.. Researchers at Shanghai Jiao Tong Universitys School of Medicine ...

Japanese scientists have found that the odorous compound responsible for halitosis - otherwise known as bad breath - is ideal for harvesting stem cells taken from human dental pulp

1. Trembley A (1744) Memoirs to serve as the story of a group of freshwater polyps with horn-shaped arms. Leiden, J & H Verbeek.. 2. Spallanzani, L. (1768), Prodromo di un opera da impnmersi sopra /a riproduzioni animali, Nella Stamperia di Giovanni Montanari, Modena. English translation by Matthew Maty as An Essay on Animal Reproductions. T. Becket and P.A De Hondt, London, 1769. 3. Baudry A, Uzunoglu E, Schneider B, Kellermann O, Goldberg M (2016) From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering. Evidence-Based Endodontics 1: 2-5.. 4. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 97: 13625-13630. [Crossref]. 5. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, et al. (2003) SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci U S A 1000: 5807-5812. [Crossref]. 6. Sonoyama W, Liu Y, Fang D, Yamaza T, Seo B, et al. (2006). Mesenchymal ...

Nichi-In* Centre for Regenerative Medicine (NCRM)institute carrying out research, clinical applications-protocol development in regenerative medicine stem cells Indias first institute for regenerative medicine

The aim of this study was to determine if diffusible angiogenic growth factors were released in human dental pulp during orthodontic tooth movement. These factors, if diffusible, could induce angiogenesis in other tissues, and may then be isolated and identified. The pulps from 14 premolar teeth treated with straight wire fixed orthodontic appliances for 2 weeks were compared with those of 14 untreated control premolar teeth from the same subjects. Following tooth extraction and sectioning, 1-mm horizontal sections of pulp tissue were embedded in collagen with l-mm sections of rat aorta and co-cultured in growth media for up to 4 weeks. Sections of rat aorta alone were also cultured. Angiogenic changes in the form of microvessel growth were observed by light microscopy. Microvessel identification was confirmed by electron microscopy and by immunohistochemistry using staining for factor VIII-related antigen marker for endothelial cells.. When compared at days 5, 10 and 14 of co-culture, the ...

NeoMTA Plus is a stainproof, tricalcium silicate-based bioactive cement that can be used universally for vital pulp and other endodontic indications in primary and permanent teeth. NeoMTA Plus is a bioceramic cement that triggers the healing process. With NeoMTA Plus, general dentists, pediatric dentists, and endodontists now have a superior and reasonably priced bioceramic. Dentists praise the multi-use NeoMTA Plus because it mixes more smoothly, is easier to dispense and the unique gel enables more stable placement, washout resistance and faster clinical setting.. New, stain-free light color:. Ideal for pediatric dentistry-wont discolor ...

CONFERENCE ON THE BIOLOGY OF THE HUMAN DENTAL PULP Classificationof pulpal pathosis Samuel Seltzer, D.D.S., Philadelphia, Pa. TEMPLE USIVERSITY SCHOOL OF DENT…

Agha-Hosseini, Farzaneh and Jahani, Mohammad-Ali and Jahani, Mohammad and Mirzaii-Dizgah, Iraj and Ali-Moghaddam, Kamran (2009) In vitro isolation of stem cells derived from human dental pulp. Clinical Transplantation, 24 (2). E23-E28. ISSN 09020063 ...

Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy

Stem cell research is a new field that is advancing at an incredible pace with new discoveries being reported from all over the world. Stem cells are powerful ...

With all of the stem cell research that has been done in the past several years, most people are probably unaware of the dental stem cells research that has been done. With some of this research, we will be able to stop tooth decay and maybe even some day regenerate or repair teeth.. It seems that the only stem cell research that we hear on the news is the very controversial embryotic stem cell research. There is much more research being done.. ...

The potential from dental pulp stem cells include developing nerve and brain tissue, repairing muscle, developing bone, developing bone and tissue from the oral cavity, reconstructing affected cardiac tissue, developing cartilage, and treating type 1 diabetes to name a few. In the past, researchers harvested stem cells taken from bone marrow to create cells of various organs, however years of research implies that dental stem cells extracted from a tooth pulp might build organ tissues more quickly. Of major relevance is that UK scientists have ascertained that stem cells derived from tooth pulp effectively prevent a leading cause of blindness caused by traumatic injuries or degenerative diseases as reported in a July 2014 study on Neural Regeneration Research (Vol. 9, No. 6, 2014), University Of Birmingham, UK ...

Researchers from the University of Adelaide, led by Dr. Kylie Ellis, have discovered that dental pulp stem cells [DPSC] have the ability to differentiate into complex networks of cells closely resembling neurons found in the brain. According to Dr. Ellis, Stem cells from teeth have great potential to grow into new brain or nerve cells, and this could potentially assist with treatments of brain disorders, such as stroke. She goes on to say ultimately, we want to be able to use a patients own stem cells for tailor-made brain therapy that doesnt have the host rejection issues commonly associated with cell-based therapies. Another advantage is that dental pulp stem cell therapy may provide a treatment option available months or even years after the stroke has occurred. Current drug treatment therapies for stroke victims must be administered almost immediately following the stroke - within hours. This severely limits their application as most stroke victims dont have access to these treatments ...

Revascularisation of the devitalised root canal is the Holy Grail of Endodontics and is being hotly pursued by many teams of clinicians but has yet to be achieved. The overall aim of this work was to attempt to induce early angiogenesis in human dental pulp stromal cells (DPSCS) in vitro and in vivo using a biomimetic approach based on combining scaffolds comprised of ECM components with DPSCs as a first step towards a tissue engineering strategy for dental pulp regeneration. After isolating DPSCs using collagenase digest, they were cultured on 1% hyaluronic acid (HyA) or Types I and III collagen matrices used either singly or in combination to determine the ability of these scaffolds to support/induce early angiogenic change in DPSCs both in vitro and in vivo. Angiogenic change was determined using a combined approach of DNA quantification, histology, immunohistochemistry to detect the angiogenic markers CD31 and CD34 and quantitative RT-PCR. DPSCs were shown to attach and proliferate on Type I ...

The article focuses on the benefits and applications of dental stem cells for dental hygiene practice and research. It mentions various types of stem cells including embryonic and somatic stem cells, and discusses their advantages and disadvantages. It gives an overview of the history of the development of dental stem cells and their potential use in the treatment of diseases including Parkinsons. Also discussed is the future of dental stem cells research and dental hygienists role in it. INSETS: Nondental Stem Cells for Dental Applications;How Stem Cells Are Extracted from Banked Tissue ...

Our study provides initial experimental and clinical evidence that hDPSCs implanted into injured incisor teeth in the absence of a scaffold promoted 3D dental pulp regeneration and partial tooth recovery. The regenerated pulp tissue contained normal structures such as an odontoblast layer, connective tissue, blood vasculature, and neuronal tissue. The regenerated pulp in the incisor teeth receiving the hDPSC implants showed functional responses to a stimulus in the electric pulp test. Notably, compared to the apexification control group, the hDPSC implant group showed an increase in root length and a reduction in the width of the apical foramen at 12 months after treatment, suggesting that regenerated dental pulp promoted tooth recovery.. Given that tooth root development depends on the residual stem cells in the apical third of the root canal, several studies have assessed the effects of pulp revascularization treatment (23-25). This approach provides stem cells from tooth periapical tissue; in ...

High purity means there are less wrong cells that are being differentiated to other tissues, or remaining as stem cells. Moreover, these facts suggest that patients undergoing transplantation with the hepatic cells may have almost no possibility of developing teratomas or cancers, as can be the case when using bone marrow stem cells, said lead author of the study Dr. Ken Yaegaki ...

The control of pain perception is a challenge in clinical dentistry, most prominent during tooth pulp inflammation. The tooth pulp is a well-defined target, and is densely supplied by a sensory trigeminal innervation. Opioids are signaling molecules that are suggested to participate in pain perception. Here we analysed the presence of delta opioid receptor (DOR) in trigeminal neurons innervating the tooth pulp of rat molars. Immunohistochemical and ultrastructural analysis revealed that DOR was identified in peripheral nerves in the molar dental pulp, both in the root and the coronal pulpal parts, with branching in the highly innervated subodontoblast layer. DOR was localised in about one third of all the trigeminal dental neurons, identified by means of retrograde neuronal transport of fluorogold (FG) from the dental pulp. Of the DOR-labeled neurons, nearly all were small and medium-sized (147.5-1,810.2 microm(2), mean 749.1 +/- 327.3 microm(2)). Confocal microscopy confirmed that DOR-immunoreactivity

Dental stem cells are highly innervated. Using the organ-on-a-chip technology, which relies on small three-dimensional devices mimicking the basic functions of human organs and tissues, the researchers demonstrated that both types of stem cells promoted neuronal growth.. The dental pulp stem cells, however, yielded better results compared to bone marrow stem cells: They induced more elongated neurons, formed dense neuronal networks and established close contacts with nerves.. Dental stem cells produce specific molecules that are fundamental for the growth and attraction of neurons. Therefore, stem cells are abundantly innervated, says Mitsiadis. The formation of such extended networks and the establishment of numerous contacts suggest that dental stem cells create functional connections with nerves of the face.. Therefore, these cells could represent an attractive choice for the regeneration of functional, properly innervated facial tissues, adds co-author and junior group leader ...

CURRENT STEM CELL NEWS. 1. Dental Pulp Cells, An optimal source for iPS-Japanese Scientists. In a paper published in the International and American Associations for Dental Researchs Journal of Dental Research, Japanese researchers have identified dental stem cells to be an optimal source for Induced Pluripotent Stem Cells as Dental Pulp stem cells are an easily accessible source . Click here for more news..... 2. Anti-HIV genes incorporated to blood cell to fight HIV infection. Researcher John Rossi and his colleagues have tried a novel way for combating HIV infection by genetically modifying the HIV-infected patients own blood stem cells and increasing the cells ability to fight off the virus . Click here for more news..... 3. A novel Injectable Bone material to deliver Stem Cells. A team of researchers at the University of Nottingham have invented a new class of materials that can be injected by surgeons as a low-viscosity fluid into the body and this material by using the body heat can ...

To ensure that your childs dental pulp stem cell collection goes smoothly, heres our guidance on how to prepare the tooth using our specialist sample collection kit

Welcome to the NDPL Video library! Below is a selection of videos about dental pulp stem cells and induced pluripotent stem cells.

TY - JOUR. T1 - Light and electron microscopic analysis of the somata and parent axons innervating the rat upper molar and lower incisor pulp. AU - Paik, S. K.. AU - Park, K. P.. AU - Lee, S. K.. AU - Ma, S. K.. AU - Cho, Y. S.. AU - Kim, Y. K.. AU - Rhyu, Im Joo. AU - Ahn, D. K.. AU - Yoshida, A.. AU - Bae, Y. C.. PY - 2009/9/15. Y1 - 2009/9/15. N2 - The morphology of intradental nerve fibers of permanent teeth and of continuously growing rodent incisors has been studied in detail but little information is available on the parent axons that give rise to these fibers. Here we examined the axons and somata of trigeminal neurons that innervate the rat upper molar and lower incisor pulp using tracing with horseradish peroxidase and light and electron microscopic analysis. The majority (∼80%) of the parent axons in the proximal root of the trigeminal ganglion that innervated either molar or incisor pulp were small myelinated fibers (,20 μm2 cross-sectional area). The remaining ∼20% of the ...