The World Health Organization estimates that dengue virus causes more than 50 million cases of dengue fever a year. Dengue virus infection is the leading cause of hospitalization and death in children of most tropical Asian countries. There are four different serotypes of dengue virus. Most cases of dengue hemorrhagic fever/dengue shock syndrome are caused by secondary infection with a dengue serotype different from the first serotype the individual was infected with. A vaccine that would be effective in preventing infection by multiple dengue serotypes is desirable. The purpose of this study is to determine the safety of and immune response to two different dengue virus vaccines in individuals who have been previously vaccinated against a different serotype.. This study will last at least 42 days. Participants will be recruited from a database of previous dengue vaccine recipients and will be stratified by the type of vaccine previously received. Participants assigned to Cohort 1 and Cohort 2 ...

It is known that antibodies to dengue viruses at subneutralizing concentrations enhance dengue virus infection of Fc gamma R+ cells. This phenomenon called antibody-dependent enhancement (ADE) occurs when virus-antibody complexes bind to the Fc gamma R via the Fc portion of the Ig. It has been hypothesized that ADE may be responsible for the pathogenesis of the severe manifestations of dengue virus infection including dengue hemorrhagic fever/dengue shock syndrome. To further analyze the mechanisms of ADE, we prepared bispecific antibodies by chemically cross-linking antidengue virus antibodies to antibodies specific for Fc gamma RI or Fc gamma RII and the non-Fc R molecules beta2 microglobulin, CD15 or CD33 and examined whether these bispecific antibodies could enhance infection. Bispecific antibodies targeting dengue virus to Fc gamma RI or Fc gamma RII enhanced dengue virus infection, consistent with previous reports using conventional antibodies. Furthermore, bispecific antibodies targeting ...

Dengue virus is a human pathogen that causes dengue fever, which can either resolve after mild fever or lead to severe dengue hemorrhagic fever/dengue shock syndrome. The role of dengue virus levels in the blood and the kinetics of infection and immune response that results in severe dengue disease in humans is not well characterized. In this study, we analyzed 97 children with varying degrees of dengue disease, and we show that the dengue virus quantity in blood does not show any significant association with severe disease. However, most severe dengue patients had lower levels of interferons and Th1 cytokines and increased levels of secreted factors such as IL-6, IL-8 and IL-10 that could potentially cause leakage in blood capillaries. Our results indicate that monocytes, which are infected with dengue virus in patients, could possibly play a major role in dengue pathogenesis. Furthermore, using computational analysis we identified association of some of the secreted factors with severe disease ...

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The dengue viruses are spread by mosquitoes and infect up to 390 million individuals annually in sub-tropical and tropical regions of the world. Dengue disease has emerged as the most important arthropod-borne viral disease of humans. Dengue virus infection generally results in dengue fever, a debilitating but self limited febrile disease. However, complications may arise, leading to the potentially fatal dengue haemorrhagic fever/dengue shock syndromes (DHF/DSS). Despite intensive research over recent years, the pathogenesis of DHF/DSS is still poorly understood and there is neither a safe and effective vaccine nor suitable anti-viral treatments to control dengue disease.. Current investigations in the laboratory focus on understanding the role of specific viral proteins in the virus lifecycle and the role they play in perturbing host cellular processes. Our studies use a dengue virus reverse genetic system, in combination with structural, biochemical and high throughput transcriptomic and ...

TY - JOUR. T1 - Role of cognitive parameters in dengue hemorrhagic fever and dengue shock syndrome. AU - Tsai, Jih Jin. AU - Chokephaibulkit, Kulkanya. AU - Chen, Po Chih. AU - Liu, Li Teh. AU - Hsiao, Hui Mien. AU - Lo, Yu Chih. AU - Perng, Guey Chuen. PY - 2013/12/5. Y1 - 2013/12/5. N2 - Dengue is becoming recognized as one of the most important vector-borne human diseases. It is predominant in tropical and subtropical zones but its geographical distribution is progressively expanding, making it an escalating global health problem of today. Dengue presents with spectrum of clinical manifestations, ranging from asymptomatic, undifferentiated mild fever, dengue fever (DF), to dengue hemorrhagic fever (DHF) with or without shock (DSS), a life-threatening illness characterized by plasma leakage due to increased vascular permeability. Currently, there are no antiviral modalities or vaccines available to treat and prevent dengue. Supportive care with close monitoring is the standard clinical ...

Dengue viruses cause dengue fever and the more severe condition, dengue hemorrhagic fever/shock syndrome. Dengue viruses are common in most tropical and subtropical regions of the world and infection with dengue viruses is the leading cause of hospitalization and death in children in many tropical Asian countries. For these reasons, the World Health Organization (WHO) has made the development of a dengue virus vaccine a top priority. This study will evaluate the safety and immunogenicity of two doses of a live attenuated, tetravalent dengue virus vaccine called TetraVax-DV in healthy adults (18-50 years old) who have previously been infected with a dengue virus or other flavivirus or have previously received a flavivirus vaccine. Two different formulations of the TetraVax-DV vaccine will be evaluated.. Participants will be randomly assigned to receive one of two admixtures of the TetraVax-DV vaccine or a placebo. At a baseline study visit (Day 0), participants will undergo a medical history ...

In the absence of truly effective and sustainable vector control measures, a dengue vaccine is urgently needed. While dengue vaccines have been under development since the 1940s, due to the limited appreciation of global dengue disease burden and of the potential markets for dengue vaccines, the industrys interest languished throughout much of the 20th century.. In recent years, however, the development of dengue vaccines has accelerated dramatically. Sanofi Pasteurs Dengvaxia® became the first dengue vaccine to be licensed for use in 2015. Today, several other vaccines are in various stages of advanced development, with clinical trials currently underway.. Vaccine development for dengue is particularly challenging because dengue fever is caused by any of four related, but distinct, virus serotypes (DENV-1-4). While infection by one virus provides lifelong immunity against that serotype, it provides only partial and transient immunity against the other three. This means that an effective ...

BACKGROUND: Sri Lanka has been affected by epidemics of dengue infections for many decades and the incidence and severity of dengue infections have been rising each year. Therefore, we investigated the age stratified seroprevalence of dengue infections in order to facilitate future dengue vaccine strategies. In addition, since the symptomatic dengue infections have increased during the past few decades, we also investigated the possible association with Japanese Encephalitis Virus (JEV) antibody seropositivity with symptomatic dengue in a community cohort in Sri Lanka. METHODS: 1689 healthy individuals who were attending a primary health care facility were recruited. Dengue and JEV antibody status was determined in all individuals and JEV vaccination status was recorded. RESULTS: 1152/1689 (68.2%) individuals were seropositive for dengue and only 133/1152 (11.5%) of them had been hospitalized to due to dengue. A significant and positive correlation was observed for dengue antibody seropositivity and age

The NEJM article reported results from a new pooled efficacy analysis of individuals 9 years of age and older at vaccination from the two Phase III studies of Sanofi Pasteurs dengue vaccine. The new analysis documented that the vaccine protects two-thirds of these individuals (66%) against dengue, providing even greater protection against two clinically relevant manifestations of dengue, namely severe dengue (93%) and prevention of hospitalizations due to dengue (80%) that account for the greatest human and economic burden of dengue in endemic countries. In addition, the dengue vaccine candidate protected volunteers 9 years of age and older who were previously exposed to dengue (82%), as well as those who were naïve to dengue (52.5%) prior to vaccination ...

Dengue fever, a viral disease transmitted by the mosquito Aedes aegypti, can spread rapidly in explosive epidemics. In Puerto Rico, dengue is a seasonal disease estimated to be responsible for more than 1,000 hospitalizations annually. Dengue transmission is influenced by the behavior of Aedes aegypti, movement and distribution of humans, and virus development within the mosquito. The role of each of these factors is poorly understood, in part, because of the absence of studies on the spatialtemporal patterns of dengue cases. Furthermore, recent failures of mosquito control programs designed to reduce dengue transmission indicate that long standing assumptions about the dispersal of dengue virus by mosquitoes are incorrect. The spatial and temporal distribution of dengue cases reported to the Centers for Disease Controls dengue surveillance system during a 1991-92 outbreak in Florida, Puerto Rico, and a normal transmission season (May 1994 - June 1995) in Ponce, Puerto Rico, were studied using ...

Medical information, Dengue hemorrhagic fever. Definition of Dengue hemorrhagic fever, symptoms of Dengue hemorrhagic fever, treatment of Dengue hemorrhagic fever, and prevention of Dengue hemorrhagic fever. Exams and Tests Dengue hemorrhagic fever.

Research recently published in PLoS Medicine challenges the dogma of the antibody-dependent enhancement model (ADE) for the development of dengue hemorrhagic fever (DHF). Dengue virus infection usually causes a severe flu like illness, although symptoms may be mild in young children. DHF, however, is a severe and sometimes fatal complication of dengue virus infection that affects about half a million people every year. DHF patients usually fall into two groups; children and adults who become infected with a second dengue virus serotype after an initial primary dengue virus infection with a different serotype, and infants with primary dengue virus infections born to mothers who have some dengue virus immunity. The current model for development of DHF in infants around 6 months old is that anti-dengue virus antibodies transferred from a dengue-immune mother to her child somehow enhance dengue virus infection, resulting in more severe symptoms (the antibody-dependent enhancement model). A ...

Via Radio New Zealand: Dengue fever cases in Vanuatu now over 300. Vanuatus Ministry of Health says the number of confirmed dengue fever cases in the country has climbed to 313. At the end of last month the number of...

Dengue IgG one step dengue antigen rapid rapid test kit IgM Ab Rapid Test Kit One Step Diagnosis Blood Testing. The Dengue IgG one step dengue antigen rapid rapid test kitIgM Ab Rapid Test Cassette (Whole Blood one step dengue antigen rapid rapid test kitSerum one step dengue antigen rapid rapid test kitPlasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood, serum, or plasma. The Dengue IgG one step dengue antigen rapid rapid test kitIgM Ab Rapid Test Cassette (Whole Blood one step dengue antigen rapid rapid test kit Serum one step dengue antigen rapid rapid test kit Plasma) is a qualitative membrane-based immunoassay for the ...

Abstract. Dengue represents an important public health issue in many tropical areas, leading to high morbidity and the employment of substantial health resources. Even though the number of fatalities related to dengue is unknown, several reports warn about the potential occurrence of severe infections and even death. The clinical spectrum of dengue is highly variable, ranging from a mild flu-like syndrome to severe disease, with shock and hemorrhage. The occurrence of bacterial superinfection, or coinfection, in patients with dengue has been noted by some authors, but the available information comes from anecdotic reports. In this study, we show the clinical and anatomopathological data of a patient infected with dengue, who subsequently died of acute multi-organic failure related to Staphylococcus aureus infection. The autopsy revealed a severe disseminated staphylococcal disease and confirmed dengue infection.

Dengue virus infection is one of the most common mosquito-borne illnesses worldwide, with nearly four billion people living in at risk areas throughout the world. Reported cases to World Health Organization continue to rise exponentially with an estimated 50 - 100 million symptomatic cases, mostly in Asia. Most clinical cases present with a self-limited viral illness, but some experience life-threatening severe disease. Four serotypes of dengue virus exist; infection by one serotype confers long lasting homotypic immunity, but does not confer heterotypic immunity to another serotype. Subsequent infection from a different dengue serotype can lead to the development of severe dengue. Antibody-dependent enhancement of dengue virus infection has been implicated in the development of severe disease. There have been various efforts to develop vaccines against dengue, including subunit vaccines that use only the viral envelope glycoprotein, or portions thereof. Those efforts are hampered by the ...

TY - JOUR. T1 - The exploration of risk factors of concurrent bacteraemia in patients critically ill with severe dengue. AU - Chen, Chin Ming. AU - Chan, Khee Siang. AU - Cheng, Kuo Chen. AU - Chou, Willy. AU - Chao, Hui Chun. AU - Yeh, Chiu Yin. AU - Yu, Wen Liang. PY - 2016/12/1. Y1 - 2016/12/1. N2 - We investigated the clinical features of intensive care unit (ICU) patients with concomitant severe dengue infection and bacteraemia to identify risk factors for this comorbidity. The records of all ICU dengue patients admitted during the period of 31 July-30 November 2015 were reviewed. Patients with concurrent bacteremia (positive bacterial blood culture within 72 h of ICU admission) were identified. ICU admission was required for 142 patients, of which 22 (15.5%) had concurrent bacteraemia. Species of the genus Streptococcus was the most common pathogens, followed by Escherichia coli then species of the genus Staphylococcus. Patients with a severe dengue infection and bacteraemia had higher ...

The new WHO 2009 classification of dengue divides suspected cases into three categories: dengue without warning signs, dengue with warning signs and severe dengue. We evaluated the WHO 2009 classification vs physicians subjective clinical diagnosis (gestalt clinical impression) in a large cohort of patients presenting to a tertiary care center in southern Sri Lanka hospitalized with acute febrile illness. We confirmed acute dengue in 388 patients (305 adults ≥ 18 years and 83 children), including 103 primary and 245 secondary cases, of 976 patients prospectively enrolled with AFI. At presentation, both adults and children with acute dengue were more likely than those with other AFI to have leukopenia and thrombocytopenia. Additionally, adults were more likely than those with other AFI to have joint pain, higher temperatures, and absence of crackles on examination whereas children with dengue were more likely than others to have sore throat, fatigue, oliguria, and elevated hematocrit and ...

Dengue virus infection in humans can lead to a wide range of clinical manifestations, from mild fever to potentially fatal dengue shock syndrome. The incidence of dengue fever is on the rise in tropical countries. Due to the increasing incidence of dengue fever worldwide, atypical manifestations of the disease are increasingly reported. In this article we report a patient with dengue haemorrhagic fever who presented with reversible splenial lesion syndrome. A 24-year-old Sri Lankan man who presented with fever and confusion was eventually diagnosed to have reversible splenial lesion syndrome based on brain imaging. Clinical, serological and haematological parameters confirmed a diagnosis of dengue haemorrhagic fever. His presentation, assessment, and management are described in this case report. Reversible splenial lesion syndrome is a condition which is radiologically characterized by reversible lesion in the splenium of the corpus callosum. It is associated with infectious and non-infectious

This research advances the fundamental understanding of how dengue virus replicates. Although platelets lack a nucleus, these tiny blood cells contain all the machinery needed to synthesize proteins and replicate RNA. The findings of this study are unprecedented and show that dengue hijacks blood platelets, commandeering the cells machinery to produce viral proteins and replicate its own genome. How this links to thrombocytopenia normally seen with dengue virus is still unclear. The possibility that viral antigens are exposed directly on platelets is now being considered for the first time. Dengue virus replicating in red blood cell units was a surprising finding as these cells do not have replication machinery.. The involvement of other residual cells found in red blood cell units is suspected. Blood donor systems worldwide are vulnerable to dengue virus. There have been five documented cases of dengue transmission by blood transfusion, all occurring in regions where dengue is present or ...

TY - JOUR. T1 - Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan. AU - Khan, Najeeb Ullah. AU - Danish, Lubna. AU - Khan, Hydayat Ullah. AU - Shah, Maryam. AU - Ismail, Muhammad. AU - Ali, Ijaz. AU - Petruzziello, Arnolfo. AU - Sabatino, Rocco. AU - Guzzo, Annunziata. AU - Botti, Gerardo. AU - Iqbal, Aqib. N1 - © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.. PY - 2020/9. Y1 - 2020/9. N2 - BACKGROUND: Dengue is a viral disease, transmitted by infected Aedes aegypti and Aedes albopictus female mosquitoes. Worldwide, 96 million infections were estimated in 2010. The dengue virus comprises four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4) which belong to the genus Flavivirus. Determining the serotypes during dengue outbreaks is crucial for its effective management in terms of diagnostics improvement and polyvalent vaccine development. The aim of the present study is to determine the prevalence rate of ...

Although endothelial cells have been speculated to be a target in the pathogenesis of dengue hemorrhagic fever (DHF), there has been little evidence linking dengue virus infection to any alteration in endothelial cell function. In this study, we show that human umbilical vein endothelial cells become activated when exposed to culture fluids from dengue virus-infected peripheral blood monocytes. Maximum activation was achieved with culture fluids from monocytes in which virus infection was enhanced by the addition of dengue virus-immune serum, thus correlating with epidemiological evidence that prior immunity to dengue virus is a major risk factor for DHF. Activation was strongest for endothelial cell expression of VCAM-1 and ICAM-1. In contrast, activation of endothelial cell E-selectin expression appeared to be more transient, as indicated by its detection at 3 h, but not at 16 h, of treatment. Treatment of monocyte culture fluids with anti-tumor necrosis factor alpha (TNF-alpha) antibody ...

Author(s): Forshey, Brett M; Reiner, Robert C; Olkowski, Sandra; Morrison, Amy C; Espinoza, Angelica; Long, Kanya C; Vilcarromero, Stalin; Casanova, Wilma; Wearing, Helen J; Halsey, Eric S; Kochel, Tadeusz J; Scott, Thomas W; Stoddard, Steven T | Abstract: BackgroundNearly half of the worlds population is at risk for dengue, yet no licensed vaccine or anti-viral drug is currently available. Dengue is caused by any of four dengue virus serotypes (DENV-1 through DENV-4), and infection by a DENV serotype is assumed to provide life-long protection against re-infection by that serotype. We investigated the validity of this fundamental assumption during a large dengue epidemic caused by DENV-2 in Iquitos, Peru, in 2010-2011, 15 years after the first outbreak of DENV-2 in the region.Methodology/principal findingsWe estimated the age-dependent prevalence of serotype-specific DENV antibodies from longitudinal cohort studies conducted between 1993 and 2010. During the 2010-2011 epidemic, active dengue cases were

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In this study the prevalence of dengue virus IgG antibodies among first-generation Surinamese immigrants was 81%. Although seroprevalence studies among Surinamese inhabitants are not available, this is comparable with the results from seroprevalence studies among populations in Latin America [10-13]. It seems like immigration to a non dengue endemic country causes little difference in dengue seroprevalence between the Surinamese immigrants and the majority of populations living in dengue-endemic areas of the Americas and the Caribbean get infected with the dengue virus.. As expected, the seroprevalence of previous DENV infection in our study was positively related to the duration participants had lived in Suriname before immigration. This is in line with studies performed among persons living in dengue-endemic countries in Latin America where they found higher seroprevalence rates of DENV antibodies by increasing age, which can be seen as a marker for duration of exposure [13, 14].. A limitation ...

Dengue is endemic in the U.S. Virgin Islands, but no outbreaks have been reported since 2005 (1). In November 2012, a school nurse in St. Croix reported suspected dengue in 27 (7%) of 369 students and staff members to the Virgin Islands Department of Health (VIDOH) and the CDC Dengue Branch in Puerto Rico. Four of 12 patient specimens sent to the CDC Dengue Branch for diagnostic testing were confirmed as dengue. Although VIDOH had observed an increase in passive dengue reporting, reliable baseline case counts were unavailable for comparison. An investigation was begun to determine the incidence of recent dengue virus (DENV) infection in schools and islandwide. A seroincidence study was conducted at six schools in addition to the index school, using a stratified two-stage cluster sampling methodology. Of 320 participants, 40 (20%) of 203 students and 20 (17%) of 118 staff members were immunoglobulin M (IgM) anti-DENV positive, indicating DENV infection within the preceding 3 months. Four students ...

Dengue is a mosquito-borne viral disease that affects 40% of the worlds population. Nearly four million U.S. citizens live in dengue-endemic areas; the most affected population resides in Puerto Rico. Data from a dengue surveillance system were used to describe all suspected cases reported in Puerto Rico in 2007. Rates of infection per 10,000 residents were calculated by age, sex, and residence. Rates and clinical outcomes were compared with those from outbreaks in 1994-1995 and 1998. In 2007, 10,508 suspected cases were reported; 52.5% persons were hospitalized, 31.8% reported hemorrhage, 2.2% had dengue hemorrhage fever, and 44 died. A total of 3,293 (33.0%) of processed specimens were laboratory positive for dengue virus (DENV); DENV-3 (1,342, 61.7%) and DENV-2 (677, 31.1%) were detected most often. The overall incidence of laboratory-positive dengue was 8.6 infections per 10,000 population. Rates were highest among persons 10-14 years of age (19.0), followed by persons 15-19 years of age (17.9) and

Carica papaya leaf juice (CPLJ) was well known for its thrombocytosis activity in rodents and dengue patients. However, the effect of CPLJ treatment on other parameters that could contribute to dengue pathogenesis such as nonstructural protein 1 (NS1) production and viremia level have never been highlighted in any clinical and in vivo studies. The aim of this study is to investigate the effect of freeze-dried CPLJ treatment on NS1 and viremia levels of dengue fever mouse model. The dengue infection in mouse model was established by inoculation of non-mouse adapted New Guinea C strain dengue virus (DEN-2) in AG129 mice. The freeze-dried CPLJ compounds were identified by Ultra-High Performance Liquid Chromatography High Resolution Accurate Mass Spectrometry analysis. The infected AG129 mice were orally treated with 500 mg/kg/day and 1000 mg/kg/day of freeze-dried CPLJ, starting on day 1 post infection for 3 consecutive days. The blood samples were collected from submandibular vein for plasma NS1 assay and

Carica papaya leaf juice (CPLJ) was well known for its thrombocytosis activity in rodents and dengue patients. However, the effect of CPLJ treatment on other parameters that could contribute to dengue pathogenesis such as nonstructural protein 1 (NS1) production and viremia level have never been highlighted in any clinical and in vivo studies. The aim of this study is to investigate the effect of freeze-dried CPLJ treatment on NS1 and viremia levels of dengue fever mouse model. The dengue infection in mouse model was established by inoculation of non-mouse adapted New Guinea C strain dengue virus (DEN-2) in AG129 mice. The freeze-dried CPLJ compounds were identified by Ultra-High Performance Liquid Chromatography High Resolution Accurate Mass Spectrometry analysis. The infected AG129 mice were orally treated with 500 mg/kg/day and 1000 mg/kg/day of freeze-dried CPLJ, starting on day 1 post infection for 3 consecutive days. The blood samples were collected from submandibular vein for plasma NS1 assay and

Carica papaya leaf juice (CPLJ) was well known for its thrombocytosis activity in rodents and dengue patients. However, the effect of CPLJ treatment on other parameters that could contribute to dengue pathogenesis such as nonstructural protein 1 (NS1) production and viremia level have never been highlighted in any clinical and in vivo studies. The aim of this study is to investigate the effect of freeze-dried CPLJ treatment on NS1 and viremia levels of dengue fever mouse model. The dengue infection in mouse model was established by inoculation of non-mouse adapted New Guinea C strain dengue virus (DEN-2) in AG129 mice. The freeze-dried CPLJ compounds were identified by Ultra-High Performance Liquid Chromatography High Resolution Accurate Mass Spectrometry analysis. The infected AG129 mice were orally treated with 500 mg/kg/day and 1000 mg/kg/day of freeze-dried CPLJ, starting on day 1 post infection for 3 consecutive days. The blood samples were collected from submandibular vein for plasma NS1 assay and

The immunopathology of severe dengue remains incompletely understood. Most patients who develop severe dengue have had prior infection with one or more dengue serotypes. When an individual is infected... more

The immunopathology of severe dengue remains incompletely understood. Most patients who develop severe dengue have had prior infection with one or more dengue serotypes. When an individual is infected... more

SINGAPORE - Dengvaxia, a dengue vaccine available here since March, could prove detrimental to people who have never been infected by dengue. Sanofi, which produces the vaccine, sent out an alert saying: For those not previously infected by dengue virus, the analysis found that in the longer term, more cases of severe disease could occur following vaccination upon a subsequent dengue infection. The French pharmaceutical giant said it will ask the health authorities in countries where it has been approved for use, such as in Singapore, to update their information on the vaccine. A spokesman for the Health Sciences Authority (HSA) said on Thursday (Nov 30) that it is working with Sanofi Pasteur to strengthen the package insert to include the warning of an increased risk of hospitalisation for dengue and clinically severe dengue in vaccinated individuals not previously in... Full article: http://www.straitstimes.com/singapore/health/dengue-vacci... ...

Dengue fever is prevalent in the world; in recent years, several outbreaks occurred in West Africa. It affects pregnant women. We aimed to assess the consequences of dengue fever on pregnant women and their fetuses during dengue epidemic in Burkina Faso. We conducted a cross-sectional study from November 1, 2015 to January 31, 2017 in 15 public and private health facilities in Ouagadougou, using secondary data. Immunochromatographic rapid test Duo detecting specific antibodies, immunoglobin M/G and /or dengue non structural antigen1 virus was used to diagnose dengue cases. Out of 399 (48%) women registered during the study period, 25 (6%) were pregnant. The average age of pregnant women was 30 years, with 18 and 45 years as extremes. The main symptoms were fever (92%) and headache (92%). Nine patients (36%) had severe dengue characterized by bleeding (16%), neurological symptoms (16%) and acute respiratory distress (8%). Eight (32%) of the 25 women had early miscarriage and 8 (32%) women gave birth to

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Anti-dengue virus immunoglobulin M kits were evaluated. Test sensitivities were 21%-99% and specificities were 77%-98% compared with reference ELISAs. dengue igg igm dengue rapid test kit price lower °C 2-30 2-8 2-8 2-8 2-8 Rapid diagnostic tests Test name Dengue duo cassette Hapalyse dengue-M PA kit Dengucheck WB SD dengue IgG/IgM Company, location Panbio Diagnostics Pentax, Tokyo dengue igg igm dengue rapid test kit price ...

Dengvaxia has been approved by Mexico, the Philippines and Brazil in Dec. 2015.. The vaccine is approved for the prevention of disease caused by four dengue serotypes in individuals aged 9-45 years old living in endemic areas.. The adoption of much-needed dengue vaccine is welcome news for our country, said Dr. Maria Lourdes Duenas, infectious disease specialist at Childrens Hospital Benjamin Bloom in El Salvador.. The burden of dengue in El Salvador has grown every year in the last decade. Access to this vaccine is essential to boost our prevention efforts dengue in the country and reduce the burden of disease . El Salvador reported more than 50,000 dengue fever cases in 2015, according to the Pan American Health Organization. Dengue serotype 1,2 and 3 were reported circulating in El Salvador last year.. This latest approval of the vaccine against dengue in El Salvador shows the speed that we are moving to a preventable disease dengue vaccine in countries where the disease burden is the ...

Costa Rica is 5th country in the world to approve the dengue vaccine, which was recently endorsed by the WHO SAGE recommendations supporting the safety, efficacy and public health value of the dengue vaccine in endemic countries -. - Vaccine needed to address precipitous rise of dengue in Costa Rica, with greater than 500% increase in incidence in 2016 already compared to last years numbers -. Costa Rica - 21st June, 2016 - Today Sanofi Pasteur, Sanofis vaccine division, announced that the National Ministry of Health of Costa Rica has approved Sanofi Pasteurs tetravalent dengue vaccine, Dengvaxia®, to protect individuals 9 to 45 years of age living in endemic areas against all four serotypes of dengue.. This approval in Costa Rica is the fourth registration of the dengue vaccine in Latin America, and the fifth in the world. Sanofi Pasteurs dengue vaccine has already been approved in Mexico, Brazil, El Salvador and the Philippines.. Dengue is a disease that is beginning to hit us hard as a ...

Dengue fever (DF) and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) are considered the most medically important and widespread mosquito-bor...

Author Summary India represents ~50% of the global population estimated to be at risk of dengue. Severe dengue, which is potentially fatal, correlates with very high virus load, reduction in platelet counts and haemorrhage. Antiviral therapy to reduce high virus load may be beneficial in attenuating disease severity. We have explored Indian traditional medicine, Ayurveda, to identify plants that could be a source of dengue inhibitory activity. We show that an alcoholic extract prepared from Cissampelos pareira Linn inhibited the replication of dengue viruses in living cells in culture and protected mice against dengue infection. It also showed antipyretic and anti-inflammatory effects. Importantly, this extract did not show any toxic effects in rats and did not affect platelets and red blood cells. This observation is critical as dengue fever is commonly treated with antipyretics. In a dengue-endemic resource-poor country as India, the C. pareira plant may serve as a source for the development of an

To describe cases of dengue virus (DENV) concurrent infections in patients from both local and international traveler visiting Bali, Indonesia. During a hospital-based study, 260 patients (from 161 local and 99 international traveler patients) were recruited. Among them, 190 were positive by DENV RT-PCR in which eight patients (five local and three international travelers) detected as having concurrent infections by two different DENV serotypes. Among the eight patients, the common dengue symptoms diagnosed were fever, headache, and myalgia. Six cases (75%) were diagnosed with dengue fever (DF) while two cases (25%) manifested with bleeding and were diagnosed with dengue hemorrhagic fever (DHF) grade 1. The DENVs concurrent infections involved all four DENV serotypes known to be circulating in Bali. Although cases of DENV concurrent infections have been implicated with severe manifestation, we observed that most of concurrent infections cases in our study were of mild clinical manifestation, that may be

This study describes the natural history of dengue virus (DENV) infection in rhesus monkeys exposed to the bites of DENV-infected Aedes aegypti mosquitoes. Dengue virus-infected mosquitoes were generated by either intrathoracic inoculation or by oral feeding on viremic blood meals. Each of the six rhesus monkeys that were fed upon by intrathoracically infected mosquitoes developed NS1 antigenemia and an IgM response; viremia was detected in 4/6 individuals. No virological or immunological evidence of DENV infection was detected in the three monkeys exposed to mosquitoes that had been orally infected with DENV. These results demonstrate the utility of mosquito-borne challenge of rhesus monkeys with DENV.

Colombian health authorities Wednesday alerted the population to an outbreak of dengue fever that has amounted to 1,600 confirmed cases since January 2012.. The ministry of health responded to a Red Cross report from Sunday that indicated the departments most affected by dengue include Tolima, Valle del Cauca, Santander, Norte de Santander and Cesar.. Colombias Red Cross representatives fear that many victims will not seek medical treatment, confusing dengue symptoms with those of a common cold.. Deputy Health Minister Fernando Ruiz told governors in the affected departments to take the necessary precautions to avoid an epidemic. We urgently need that these resources be put in to place to have the ability to contract the personnel that we need.. The deputy minister also called on the public to be vigilant, cautioning people to be careful with standing water tanks where there may be mosquitos that generate the dengue virus.. Symptoms of dengue include cold-like symptoms as well as sudden ...

Link to Pubmed [PMID] - 30753180. PLoS Negl Trop Dis 2019 Feb;13(2):e0007116. BACKGROUND: Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru.. METHODOLOGY/PRINCIPAL FINDINGS: Our study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. ...

Dengue can cause plasma leakage that may lead to dengue shock syndrome (DSS). In approximately 30% of DSS cases, recurrent episodes of shock occur. These patients have a higher risk of fluid overload, respiratory distress and poor outcomes. We investigated the association of echocardiographically-derived cardiac function and intravascular volume parameters plus lactate levels, with the outcomes of recurrent shock and respiratory distress in severe dengue.We performed a prospective observational study in Paediatric and adult ICU, at the Hospital for Tropical Diseases (HTD), Ho Chi Minh City, Vietnam. Patients with dengue were enrolled within 12 hours of admission to paediatric or adult ICU. A haemodynamic assessment and portable echocardiograms were carried out daily for 5 days from enrolment and all interventions recorded. 102 patients were enrolled; 22 patients did not develop DSS, 48 had a single episode of shock and 32 had recurrent shock. Patients with recurrent shock had a higher enrolment pulse

New results from an early-stage clinical trial of a dengue vaccine co-developed by researchers at the University of Vermont (UVM), Johns Hopkins University and the National Institutes of Health, bring positive news for the reported 50 to 100 million individuals infected annually with the deadly virus. According to the findings, the vaccine is safe and stimulates a strong immune response in most vaccine recipients.. The study appears in the March 15, 2013 issue of the Journal of Infectious Diseases.. Transmitted to humans by Aedes mosquitoes and prevalent in many tropical and subtropical regions of the world, dengue infection is caused by any of four related viruses: Dengue viruses (DENV) 1 through 4. Classically described dengue fever includes fever, headache, severe joint and muscle pain, and rash. Severe dengue infections, dengue hemorrhagic fever, and dengue shock syndrome include a higher risk of complications and death and are more commonly seen after a second infection with dengue viruses. ...

OBJECTIVES: A positive tourniquet test is one of several clinical parameters considered by the World Health Organization to be important in the diagnosis of dengue haemorrhagic fever, but no formal evaluation of the test has been undertaken. As many doctors remain unconvinced of its usefulness, this study was designed to assess the diagnostic utility of both the standard test and a commonly employed modified test. METHODS: A prospective evaluation of the standard sphygmomanometer cuff tourniquet test, compared with a simple elastic cuff tourniquet test, was carried out in 1136 children with suspected dengue infection admitted to a provincial paediatric hospital in southern Viet Nam. RESULTS: There was good agreement between independent observers for both techniques, but the sphygmomanometer method resulted in consistently greater numbers of petechiae. This standard method had a sensitivity of 41.6% for dengue infection, with a specificity of 94.4%, positive predictive value of 98.3% and negative

Imported dengue cases pose the public health risk for local circulation in European areas, especially southeast France, where the Aedes mosquito is established. Using a capture-recapture method with Chaos estimator, we estimated the annual incidence of dengue fever and the completeness of existing mandatory notification and laboratory network surveillance systems. During 2007-2010, >8,300 cases with laboratory evidence of recent dengue infection were diagnosed. Of these cases, 4,500 occurred in 2010, coinciding with intense epidemics in the French West Indies. Over this 4-year period, 327 cases occurred in southeast France during the vector activity period. Of these, 234 cases occurred in 2010, most of them potentially viremic. Completeness of the mandatory notification and laboratory network systems were ≈10% and 40%, respectively, but higher in southeast areas during May-November (32% and 69%, respectively). Dengue surveillance systems in France provide complementary information that is

Dengue vaccine development has made significant strides, but a better understanding of how vaccine-induced immune responses correlate with vaccine efficacy can greatly accelerate development, testing, and deployment as well as ameliorate potential risks and safety concerns. Advances in basic immunology knowledge and techniques have already improved our understanding of cell-mediated immunity of natural dengue virus infection and vaccination. We conclude that the evidence base is adequate to argue for inclusion of assessments of cell-mediated immunity as part of clinical trials of dengue vaccines, although further research to identify useful correlates of protective immunity is needed.

A new approach for dengue control has been proposed that relies on life-shortening strains of the obligate intracellular bacterium Wolbachia pipientis to modify mosquito population age structure and reduce pathogen transmission. Previously we reported the stable transinfection of the major dengue ve …

Three tetravalent formulations of chimeric dengue (DENVax) viruses containing the pre-membrane and envelope genes of serotypes 1-4 expressed by the attenuated DENV-2 PDK-53 genome were tested for safety, immunogenicity, and efficacy in cynomolgus macaques (Macaca fascicularis). Subcutaneous injection of the DENVax formulations was well-tolerated. Low levels of viremia of only one of the four vaccine viruses were detected yet virus neutralizing antibody titers were induced against all four dengue virus serotypes after one or two administrations of vaccine. All animals immunized with the high-dose formulation were protected from viremia, and all immunized animals were completely protected from DENV-3 and DENV-4 challenge. A lower dose of DENVax formulation partially protected animals from DENV-1 or DENV-2 challenge. In contrast, all control animals developed high levels of viremia for multiple days after challenge with DENV 1-4. This study highlights the immunogenicity and efficacy of the ...

Objectives:To study the presentation of pleural effusion among dengue Patients in Pakistan and to explore limitations in the diagnosis of these Patients by the physicians of a developing country. Methods: We conducted a cross-sectional study on Patients admitted to Aga Khan University Hospital, Karachi, Pakistan from January 2005 to September 2008. Records of 663 dengue Patients were reviewed. Demographic data, respiratory complaints, radiographic studies, and final outcome were studied. Presentation and radiological modality confirming the diagnosis of pleural effusion were noted. Descriptive statistics followed by Chi-Square test were applied, testing against an alpha of 0.05 and 95% confidence interval (CI). Results: Three hundred and fifty-four (53%) out of a total of 663 dengue syndrome Patients did not complain of any respiratory symptoms at presentation, nor did their medical examination elicit any suspicion of PE. Hence, no radiological study was advised. Amongst the remaining 309 Patients, 299

It is imperative to control mosquitoes in order to prevent mosquito-borne diseases and improve public health. Aedes aegypti is the primary vector of dengue, dengue hemorrhagic fever, and chikungunya. Dengue fever is endemic in south-east Asia including India, Bangladesh, and Pakistan [7]. Dengue fever has become an important public health problem as the number of reported cases continues to increase, especially with more severe forms of the disease such as dengue hemorrhagic fever and dengue shock syndrome or with unusual symptoms such as central nervous system involvement8 and 9. Culex quinquefasciatus is an important vector of lymphatic filariasis in tropical and subtropical regions. It is a pantropical pest and urban vector of Wuchereria bancrofti[10] and is possibly the most abundant house mosquito in towns and cities of tropical countries. According to [11], about 90 million people worldwide are infected with W. bancrofti, and 10 times more people are at risk of being infected. In India ...

Last weeks Bahamas notice coincides with another CDC update, noting that dengue is the most common cause of fever in travelers returning from the Caribbean, Central America, and South Central Asia. As of August 5, more than 890,000 cases have been reported to the Pan American Health Organization this year, including 10,840 cases of sometimes fatal dengue hemorrhagic fever and/or dengue shock syndrome. It is widespread in Puerto Rico, and several countries across the region are reporting high incidence rates in addition to the Bahamas, including Brazil, Paraguay, Bolivia, and Aruba. Cases have also been confirmed in Florida and Hawaii ...

Dengue virus (DENV) is the most common arbovirus disease, with wide spectrum of presentation. Spinal cord involvement in dengue infection (DF) is rare. However, the risk of transverse myelitis (TM) following Dengue has not been systematically assessed. Methods: We undertook a systematic review of the English literature published from January 1974 to December 2017 to assess risk of TM and outcomes following DF. Data sources included MEDLINE, EMBASE Cochrane library, and references within identified articles. Results: We identified 242 potential studies, 62 were duplicates. A further 136 were excluded on the basis of title and abstract and 19 studies did not meet the eligibility criteria on full text screening. We included 25 publications involving 2672 cases of DF. 10.8% (289/2672) had neurological complications, of which 2.3% (61/2672) was TM. For articles reporting epidemiological data, the neurological complication was twice in males compared to female 67.7% (130/192) vs 32.7% (62/192) and 1

Effect of Dengue Hemorrhagic Fever on Thrombomodulin Level - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Effect of Dengue Hemorrhagic Fever on Thrombomodulin Level

HAWAII ISLAND - The number of locally-acquired cases of dengue fever on Hawaii Island has risen to 92, up from the 79 reported on Friday. The health department did not update the number of those infected with the mosquito-borne illness over the weekend.. Of the confirmed cases, 79 are Hawaii residents and 13 are visitors. 70 cases have been adults; twenty-two have been children under 18 years of age. Onset of illness has ranged between 9/11/15 - 11/17/15.. As of today, a total of 143 reported potential cases have been excluded based on test results and/or not meeting case criteria, the state says.. Health officials conducted preventive spraying or treating of areas around various school in the Kona and Hilo areas on Saturday, including the Kealakehe Intermediate School as was recommended by concerned lawmakers at a legislative briefing on the outbreak. Officials planned to conduct spraying at the Konawaena High School on Sunday. Hawaii County Civil Defense said there are no cases directly ...

Dengue fever (DF) is an arthropod-borne disease caused by dengue virus (DENV). DENV is a member of the genus Flavivirus in the family Flaviviridae. Recently, DENV has been reported as an important emerging infectious viral pathogen in Sudan. Multiple outbreaks and sporadic cases of DF have been frequently reported in the eastern region of Sudan. The present study was conducted to confirm DENV outbreak in Kassala State, eastern Sudan, 2019, and to provide some information on the molecular characterization of the DENV isolate associated with the disease outbreak. A hundred serum samples were collected during the outbreak from residents of Kassala State, Sudan, 2019. ELISA was used to detect DENV non structural protein NS1 (DENV-NS1) in acute phase sera sampled during the disease outbreak. RT-PCR assays were used to amplify a fragment of the capsid/pre-membrane region (CprM) of the viral polyprotein gene. The PCR products of the amplified CprM region of the viral polyprotein gene were purified and partial

Dengue viruses (DENVs) are mosquito-borne flaviviruses that are estimated to infect 390 million people each year. Dengue is a major global public health concern because people infected with the virus can develop dengue fever or severe dengue hemorrhagic fever and shock syndrome. Vaccines offer the best hope for controlling the current global DENV pandemic. The major goal of my thesis project was to define the properties of neutralizing and protective human antibodies stimulated by natural DENV infections and the leading live attenuated DENV vaccines. There are four antigenically distinct DENV serotypes, named DENV1 through DENV4. For my studies, I focused on DENV2 as a model to understand human protective immunity following infection or vaccination. Following natural DENV2 infections, individuals generate strongly neutralizing DENV2 serotype-specific antibodies, which provide protection against subsequent DENV2 infections. I characterized the properties and specific epitopes of multiple human ...

Recently there have been news stories on a dengue strain that affects the liver. This condition is also called dengue hepatitis. It leads to severe organ malfunction and in some cases even organ failure. In the case of dengue hepatitis, fever lasts longer, often for more than a week, and leaves the patient extremely exhausted. An indication of this condition, according to experts is the sudden rise in SGOT (Serum Glutamic Oxaloacetic Transaminase) and SGPT (Serum Glutamic Pyruvic Transaminase) levels, sometime almost 20 times higher than the normal level. Dengue hepatitis is characterized by high fever, lasting more than a week, extreme weakness. The blood tests show an increase in SGPT and SGOT levels, a drop in platelet count and WBCs ...

Dengue is of great concern in various parts of the world, especially in tropical and subtropical countries where the mosquito vectors Aedes aegypti and Aedes albopictus are present. The transmission of this virus to humans, by what is known as horizontal transmission, occurs through the bite of infected females of one or other of the two mosquito species. Furthermore, an infected female or male parent, by what is known as vertical transmission, can transfer this arbovirus to some part of their offspring. Considering that vertical transmission may represent an important strategy for maintaining the circulation of arboviruses in nature, the verification of this phenomenon worldwide is extremely important and necessary to better understand its dynamic. In the present study, we conducted a literature review of the presence of natural vertical transmission of dengue virus in Ae. aegypti and Ae. albopictus worldwide. Searches were conducted in MEDLINE, sciELO and Lilacs and all the studies published in

0% of all deaths caused by dengue fever this year. According to the Ministry of Health, 295 people have died of mosquito-borne diseases in Brazil since January . In Rio Preto, 2,536 new cases were confirmed in the city in one week. The current numbers of the disease were released on Friday, the 14th by the health department of the community. There are also 3,733 cases investigated. Rio Preto also counts 12 dengue deaths this year. This years epidemic is already the heaviest in history and has in some cases surpassed the big epidemic of 2010, when the city had 24,296 cases. Bauru has confirmed 2,491 new cases in one week and seven more deaths. According to a report released on Friday, there are now 22,484 cases and 28 deaths. The Ministry of Health reported that the cases occurred between the end of January and the 20th of April this year, but only now are the results of the tests available. According to the agency, most deaths occurred in the elderly and in people with chronic diseases. Dengue ...

The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification of infection, which is pivotal for disease management and the development of blood screening assays. Non-structural protein 1 (NS1) is an early surrogate marker of infection and its detection in serum coincides with detectable viraemia. The aim of this work was to isolate and characterise serotype-specific monoclonal antibodies that bind to NS1 for each of the four DENV serotypes. This was achieved using phage display and a subtractive biopanning strategy to direct the antibody selection towards serotype-specific epitopes. This antibody isolation strategy has advantages over immunisation techniques where it is difficult to avoid antibody responses to ...

Meksianis Zadrak Ndii, Roslyn I Hickson, Geoffry N Mercer Abstract Infecting Aedes aegypti mosquitoes with the bacteria Wolbachia has been proposed as an innovative new strategy to reduce the transmission of dengue fever. Field trials are currently being undertaken in Queensland, Australia. However, few mathematical models have been developed to consider the persistence of Wolbachia-…

TY - JOUR. T1 - Membrane topology and function of dengue virus NS2A protein. AU - Xie, Xuping. AU - Gayen, Shovanlal. AU - Kang, Cong Bao. AU - Yuan, Zhiming. AU - Shi, Pei Yong. PY - 2013/4/1. Y1 - 2013/4/1. N2 - Flavivirus nonstructural protein 2A (NS2A) is a component of the viral replication complex that functions in virion assembly and antagonizes the host immune response. Although flavivirus NS2A is known to associate with the endoplasmic reticulum (ER) membrane, the detailed topology of this protein has not been determined. Here we report the first topology model of flavivirus NS2A on the ER membrane. Using dengue virus (DENV) NS2A as a model, we show that (i) the N-terminal 68 amino acids are located in the ER lumen, with one segment (amino acids 30 to 52) that interacts with ER membrane without traversing the lipid bilayer; (ii) amino acids 69 to 209 form five transmembrane segments, each of which integrally spans the ER membrane; and (iii) the C-terminal tail (amino acids 210 to 218) ...

THE Ministry of Health has advised the public of the possible existence of two new mosquito-borne viral diseases - chikungunya and zika.. It has also confirmed there is no vaccine for these two diseases, which is transmitted by infected mosquitoes.. The ministrys national adviser for communicable diseases, Dr Mike Kama, said they feared the chikungunya and zika viruses, which have been identified in other Pacific island countries, may follow the same path as dengue.. Dengue fever killed 15 people and infected 25,300 people in the past seven months, according to statistics released by the ministry.. Dr Kama said there had been no cases of chikungunya or zika virus identified in the country so far but warned that despite the decline in the number of dengue fever cases, citizens must remain vigilant. Regular blood samples have been sent abroad for test for these emerging mosquito-borne viruses.. We were free from dengue for a year before it started knocking on our doors in December, so theres ...

SINGAPORE, April 21 /PRNewswire/ -- - New Studies Advance the Development of Novel Vaccine for Dengue Prevention in Asia Pacific SINGAPORE, April 21 /PRNewswire/ -- Sanofi Pasteur, the vaccines division of sanofi-aventis Group (EURONEXT : SAN and NYSE : SNY), announced today the start of clinical studies of its investigational tetravalent dengue va

Sphingosine kinase 1 (SphK1) is a lipid kinase with important roles including regulation of cell survival. We have previously shown reduced SphK1 activity in cells with an established dengue virus type-2 (DENV-2) infection. In this study, we examined the effect of alterations in SphK1 activity on DENV-2 replication and cell death and determined the mechanisms of the reduction in SphK1 activity. Chemical inhibition or overexpression of SphK1 after established DENV-2 infection had no effect on infectious DENV-2 production, although inhibition of SphK1 resulted in enhanced DENV-2-induced cell death. Reduced SphK1 activity was observed in multiple cell types, regardless of the ability of DENV-2 infection to be cytopathic, and was mediated by a post-translational mechanism. Unlike bovine viral diarrhea virus, where SphK1 activity is decreased by the NS3 protein, SphK1 activity was not affected by DENV-2 NS3 but, instead, was reduced by expression of the terminal 396 bases of the 3′ UTR of DENV-2 RNA. We

Salutary effects of carica papaya leaf extract in dengue fever patients- a pilot study... Salutary effects of carica papaya leaf extract in dengue fever patients- a pilot study.. Thrombocytosis and anti-inflammatory properties, and toxicological evaluation of carica papaya mature leaf concentrate in a murine model... A study of haemato-stimulatory effects of graded dose of aqueous carica papaya seed extract in wistar rats... Dengue fever treatment with carica papaya leaves extracts... Carica papaya leaves juice significantly accelerates the rate of increase in platelet count among patients with dengue fever and dengue haemorrhagic fever... The effect of carica papaya l. Leaves extract capsules on platelets count and hematocrit level in dengue fever patient... Evaluation of platelet augmentation activity of carica papaya leaf aqueous extract in rats.. ...

21st Century Malaysia Repella - Anti Dengue, Anti Zika Repellent Cream [4oz] - 21ST Century HERBAL MOSQUITO REPELLENT is an effective, absolutely harmless, natural, moisturizing repellent that protects against Dengue Fever, Dengue Hemorrhagic Fever, Malaria and other mosquito borne diseases. A natural insect repellent made from 100% NATURAL HERBS that repel mosquitoes. It contains NO CHEMICALS and NO DIETHYL-TOLUAMIDE making it completely SAFE

Part B - Final decisions on matters not referred to an expert advisory committee New Chemical Entities - medicines for human therapeutic use

The Philippines decided to suspend its dengue immunization program because the used vaccine poses health risks in people who were not previously infected.. The decision comes after the announcement of the French pharmaceutical manufacturer Sanofi (link) that its vaccine, Dengvaxia, is efficient only if the subject had previously had been infected. Otherwise, more cases of severe disease could occur.. These findings highlight the complex nature of dengue infection. We are working with health authorities to ensure that prescribers, vaccinators and patients are fully informed of the new findings, with the goal of enhancing the impact of Dengvaxia in dengue-endemic countries, said Dr. Su-Peing Ng, Global Medical Head, Sanofi Pasteur.. The Health secretary, Mr. Francisco Duque, said that the suspension will not be revoked until the government consults medical experts and the World Health Organization.. 740,000 school students have already received the vaccine as part of a national program, and ...

The results presented in this report demonstrated that four ISGs, IFITM2, IFITM3, viperin, and ISG20, were able to significantly inhibit both WNV and DENV infections. Considering the similarity in the genome structures and replication strategies of the two viruses, it is not surprising that similar sets of ISGs inhibited the replication of both viruses. However, it is interesting that PKR could inhibit WNV infection but not DENV infection. Although the inability of PKR to inhibit DENV replication is consistent with a previous report demonstrating that IFN-α inhibited DENV infection of cells by preventing translation of viral RNA through a PKR-independent mechanism (10), it will be interesting to know how PKR could selectively recognize and respond to WNV infection but not DENV infection.. Viperin is a putative radical SAM domain-containing enzyme associated with the endoplasmic reticulum through an amphitropic alpha helix structure located within the N-terminal 45 amino acid residues of the ...

According to an October 11 Viewpoint article in theJournal of the American Medical Association, nearly 1700 cases of travel-associated Zika infection, including 479 in pregnant women, had been reported in the continental United States as of August 4, 2016. University of Vermont Vaccine Testing Center researchers Sean Diehl, Ph.D., assistant professor of medicine, and Kristen Pierce, M.D., associate professor of medicine, will discuss their role in the development of a dengue virus vaccine and how that work has been helpful in the development of a vaccine to defend against the Zika virus at Community Medical School on Tuesday, November 1 at 6 p.m. Titled The Dengue Fever Vaccine: How It Can Help Us Defend Against the Zika Virus, the lecture will take place in a different location this session - the Sullivan Classroom in the Larner Medical Education Center atthe Larner College of Medicine at the University of Vermont.. Organized jointly by the Larner College of Medicine and the UVM Medical ...

Dengue fever symptoms include skin rashes and severe fatigue. A proven remedy is papaya leaf extracts, which increase platelet count, in those affected by this viral illness. Learn more, about dengue fever causes, indications and treatment.

...Researchers in South East Asia have identified two genetic variants as...Dengue is globally the most common mosquito-borne infection after mala...In children severe dengue is characterised by increased vascular perm...To test this hypothesis researchers at the Wellcome Trust Vietnam Res...,First,genome-wide,association,study,for,dengue,identifies,candidate,susceptibility,genes,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters

Health officials in Costa Rica said an outbreak of dengue fever has sickened 7,000 people, with many cases occurring in some of this Central American countrys most popular tourist areas.

Three billion people around the world live in tropical and subtropical regions where the four strands -or serotypes- of the denge virus (see map) exist. Each year, between 50 to 100 million people are infected through the bite of the female Aedes aegypti and other similar species, such as Aedes albopictus.. In the last decades, the rapid growth of urban populations, coupled with poor environmental sanitation services and a lack of adequate water-storage resources, has favoured the spread of mosquito vectors. At the same time, the increase in international air traffic has contributed towards the geographic dissemination of the viruses. These factors have led to a re-emergence of dengue fever, dengue hemorrhagic fever and dengue shock syndrome epidemics. Hyperendemicity (when few members of a single community are not infected) has also increased in several urban centres.. There are currently over 100 countries where dengue is endemic and the number is rising. With no available vaccine or specific ...

SUPPLEMENT: The four serotypes of dengue virus (DENV) cause 100 million cases of dengue fever (DF) yearly. In ~500,00 cases, infection with DENV results in a severe disease, previously known as dengue hemorrhagic fever (DHF) causing 20,000 annual deaths 1-3. Five percent of cases of DHF affect infants during primary infections 4. Since the 1980s, cases of DHF expanded from SE Asia to other regions 1-3. In Northern Argentina, 25,000 cases of dengue were reported in 2009 5. An important consequence of this problema is that unlike in SE Asia, where ~99% of women of childbearing age are immune to DENV and transfer protective antibody to their babies through the placenta 6, susceptible fetuses and infants in Northern Argentina experience primary DENV infection while lacking maternal antibody to modulate disease. This situation has led to infrequent and severe clinical manifestations in neonates and infants. In recent years, we detected infants with severe congenital DENV infection 7 and immature ...

KIT, LAM SAI (2015) Region-wide synchrony and traveling waves of dengue across eight countries in Southeast Asia. PNAS, 112 (42). 13069 13074. KIT, LAM SAI (2013) Challenges in reducing dengue burden; diagnostics, control measures and vaccines. Expert Review of Vaccines, 12. KIT, LAM SAI (2013) Gynura procumbens Causes Vasodilation by Inhibiting Angiotensin II and Enhancing Bradykinin Actions. Journal of Cardiovascular Pharmacology, 61. KIT, LAM SAI (2013) Potassium channel openers and prostacyclin play a crucial role in mediating the vasorelaxant activity of Gynura procumbens. Bmc Complementary and Alternative Medicine, 13. KIT, LAM SAI (2012) Call for a World Dengue Day. The Lancet, 379 (9814). pp. 411-412. KIT, LAM SAI (2011) Preparing for introduction of a dengue vaccine: Recommendations from the 1st Dengue v2V Asia-Pacific Meeting. Vaccine, 2951. KIT, LAM SAI (2011) Preparing for introduction of a dengue vaccine: Recommendations from the 1st Dengue v2V Asia-Pacific Meeting. Vaccine, 29. ...