TY - JOUR. T1 - Endometrial stromal decidualization responds reversibly to hormone stimulation and withdrawal. AU - Yu, Jie. AU - Berga, Sarah L.. AU - Johnston-MacAnanny, Erika B.. AU - Sidell, Neil. AU - Bagchi, Indrani C.. AU - Bagchi, Milan K.. AU - Taylor, Robert N.. PY - 2016/6. Y1 - 2016/6. N2 - Human endometrial stromal decidualization is required for embryo receptivity, angiogenesis, and placentation. Previous studies from our laboratories established that connexin (Cx)-43 critically regulates endometrial stromal cell (ESC) differentiation, whereas gap junction blockade prevents it. The current study evaluated the plasticity of ESC morphology and Cx43 expression, as well as other biochemical markers of cell differentiation, in response to decidualizing hormones. Primary human ESC cultureswere exposedto 10 nM estradiol, 100 nM progesterone, and 0.5 mM cAMPfor up to 14 days, followed by hormone withdrawal for 14 days, mimicking a biphasic ovulatory cycle. Reversible differentiation was ...
Methods have been developed for isolating human tissue macrophages from first trimester or term pregnancy decidua. After a two stage enzymic digestion, viable cells were separated from cellular debris by velocity sedimentation at unit gravity or by Percoll centrifugation. Cell populations were analysed by flow cytometry after labelling with monoclonal antibodies. In term decidua, 47% of the cells were of bone marrow origin, comprising 18% macrophages, 3% large granular lymphocytes and 8% T cells. The remaining cells, the proportion of which varied between individuals, were CD16-positive granulocytes. Macrophages were isolated flow cytometrically from both first trimester and term decidual cell dispersions after labelling with an antibody to MHC class II. Yields of up to 4 X 10(6) macrophages, greater than 95% pure, were routinely obtained.
इस अध्ययन के एक सत्यापित और अलगाव और मानव एंडोमेट्रियल stromal कोशिकाओं की संस्कृति के लिए प्रक्रिया को इन विट्रो...
A successful pregnancy requires that the maternal immune system adapts to tolerate the semi-allogeneic fetal-placental unit. This adaptation mainly occurs locally, i.e. at the fetal-maternal interface, where fetal-derived tissues come into close contact with maternal cells in the uterine endometrium (called decidua during pregnancy). Macrophages and regulatory T (Treg) cells are maternal immune cells that are enriched in the decidua and they likely play a central role in promoting fetal tolerance. However, the precise function of decidual macrophages and the factors regulating both macrophages and Treg cells in humans are unknown. The aim of this thesis was to characterize the phenotype and function of decidual macrophages from first trimester human pregnancy and to identify factors responsible for inducing tolerogenic properties in both decidual macrophages and Treg cells. CD14+ decidual macrophages showed characteristics of immune suppressive or homeostatic macrophages (expression of CD163, ...
TY - JOUR. T1 - Expression of the interleukin-2 receptor α (CD25) is selectively decreased on decidual CD4 + and CD8 + T lymphocytes in normal pregnancies. AU - Chao, Kunag Han. AU - Wu, Ming Yih. AU - Yang, Jehn Hsiahn. AU - Chen, Shee Uan. AU - Yang, Yu Shih. AU - Ho, Hong Nerng. PY - 2002/7/22. Y1 - 2002/7/22. N2 - In a previous study, we demonstrated that the proportion of activated T cells (CD69 +CD3 + and HLA-DR +CD3 +) is higher in the endometrium and decidua after the luteal phase and throughout early pregnancy compared with in the peripheral blood. However, there was no difference in the proportion of CD25 +CD3 + lymphocytes between the endometrium and peripheral blood. In this study, we further verify that the levels of CD25 on CD4 + and CD8 + T lymphocytes are not increased in normal pregnancy, although the levels of CD69 and HLA-DR are markedly increased. We also elucidate that the amounts of all three activation molecules on local T lymphocytes are down-regulated in pregnancy ...
Objective: One of the most serious complications after hematopoietic stem cell transplantation is acute graftversus- host disease (GVHD), which develops when donor- derived T-cells recognize and become activated by alloreactive recipient tissue. We were the first to use bone marrow-derived mesenchymal stem cells (BM-MSCs) to reverse severe steroid-refractory acute GVHD and hemorrhagic cystitis. The fetus is protected against the mothers immune system by the fetal membrane. We wanted to explore if stromal cells from the placenta could be used for GVHD and tissue damage in a similar way as MSCs. Materials and Methods: We collected stromal cells from various parts of the placenta. To characterize these cells, we used fluorescence activated cell sorter (FACS). For functional analysis, mixed lymphocyte culture (MLC) and mitogen stimulation of human and mice cells were used. For studies of GVHD, we used B6 mice as donors and Balb/C mice as recipients of hematopoietic stem cell transplants. Patients ...
BackgroundAlthough uterine macrophages are thought to play an important regulatory role at the maternal-fetal interface, their global gene expression profile is not known.Methodology/Principal FindingsUsing micro-array comprising approximately 14,000 genes, the gene expression pattern of human first trimester decidual CD14+ monocytes/macrophages was characterized and compared with the expression profile of the corresponding cells in blood. Some of the key findings were confirmed by real time PCR or by secreted protein. A unique gene expression pattern intrinsic of first trimester decidual CD14+ cells was demonstrated. A large number of regulated genes were functionally related to immunomodulation and tissue remodelling, corroborating polarization patterns of differentiated macrophages mainly of the alternatively activated M2 phenotype. These include known M2 markers such as CCL-18, CD209, insulin-like growth factor (IGF)-1, mannose receptor c type (MRC)-1 and fibronectin-1. Further, the selective up
In cycling rodents, the circulating E and P are produced and secreted by the concerted actions of ovarian granulosa and theca cells (10). The adrenal glands in rodents are incapable of synthesizing significant levels of these steroid hormones (11). In pregnant mice, the corpora lutea develop fully by d 3 of pregnancy and start to produce P, the level of which increases and remains elevated until mid-gestation (12). In contrast, the level of serum E, which is high on d 1 of pregnancy because of the preovulatory hormonal surge, decreases and reaches approximately 15 pg/mL on d 2 and 3. The serum E level increases transiently to approximately 22 pg/mL on d 4 of pregnancy and plays a critical role in embryo attachment. When the embryo attaches to the uterus, the ovarian E synthesis decreases. During the decidualization phase, which lasts from d 5 through 8 of gestation, the circulating level of E remains low at approximately 15 pg/mL (12). When the ovaries were removed after embryo attachment, ...
Introduction: At the maternal fetal interface, the decidua may play a role in labor, a process which involves the prostaglandin (PG) pathway (important clinical target for labor management). However, the PG pathway is highly complex with three rate limiting steps, and its complexity remains to be addressed in the decidua. We hypothesize the decidua exhibits 1) a distinct expression profile of PG genes with labor, and 2) differential expression in preterm labor (PTL) compared to term labor ...
Abstract: In early human pregnancy, uterine decidual NK cells (dNK) are abundant and considered as cytokine producers but poorly cytotoxic despite their cytolytic granule content, suggesting a negative control of this latter effector function. To investigate the basis of this control, we examined the relative contribution to the cytotoxic function of different activating receptors expressed by dNK. Using a multicolor flow cytometry analysis, we found that freshly isolated dNK exhibit a unique repertoire of activating and inhibitory receptors, identical among all the donors tested. We then demonstrated that in fresh dNK, mAb-specific engagement of NKp46-, and to a lesser extent NKG2C-, but not NKp30-activating receptors induced intracellular calcium mobilization, perforin polarization, granule exocytosis and efficient target cell lysis. NKp46-mediated cytotoxicity is coactivated by CD2 but dramatically blocked by NKG2A coengagement, indicating that the dNK cytotoxic potential could be tightly ...
Uncovering the causes of pregnancy complications such as preterm labor requires greater insight into how the uterus remains in a noncontractile state until term and then surmounts this state to enter labor. Here, we show that dynamic generation and erasure of the repressive histone modification tri-methyl histone H3 lysine 27 (H3K27me3) in decidual stromal cells dictate both elements of pregnancy success in mice. In early gestation, H3K27me3-induced transcriptional silencing of select gene targets ensured uterine quiescence by preventing the decidua from expressing parturition-inducing hormone receptors, manifesting type 1 immunity, and most unexpectedly, generating myofibroblasts and associated wound-healing responses. In late gestation, genome-wide H3K27 demethylation allowed for target gene upregulation, decidual activation, and labor entry. Pharmacological inhibition of H3K27 demethylation in late gestation not only prevented term parturition, but also inhibited delivery while maintaining ...
Even after intensive study, many of the maternal immunological adaptations to pregnancy are not well elucidated. It is known that pregnancy is associated with an increase in the CD4+ T cells that secrete Th2-type cytokines-for example interleukins (Fragiadakis, 2016). Th1-type cytokine production-for example, interferon gamma and interleukin 2-appears to be somewhat suppressed, leading to a Th2 bias in pregnancy. This bias affects the ability to rapidly eliminate certain intracellular pathogens during pregnancy, although the clinical implications of this suppression are unknown (Kourtis, 2014; Svensson-Arvelund, 2014). Importantly, the Th2 humoral immune response remains intact. It also appears that human leukocyte antigen (HLA)-C expressed by extravillous trophoblasts elicits responses from decidual natural killer (dNK) and decidual CD8+ T cells (Crespo, 2017). ...
Nancy P, Tagliani E, Tay CS, Asp P, Levy DE, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012 Jun 8;336(6086):1317-21. PubMed PMID: 22679098 ...
DNK (citozin-5-)-metiltransferaza (EC 2.1.1.37, EcoRI metilaza, DNK 5-citozinska metilaza, DNK citozinska C5 metilaza, DNK citozinska metilaza, DNK metilaza, DNK metiltransferaza, DNK transmetilaza, DNK-citozinska 5-metilaza, DNK-citozinska metiltransferaza, HpaII metilaza, HpaII metilaza, M.BsuRIa, M.BsuRIb, Tip II DNK metilaza, citozinska 5-metiltransferaza, citozinska DNK metilaza, citozinska DNK metiltransferaza, citozin-specifična DNK metiltransferaza, dezoksiribonukleinska metilaza, dezoksiribonukleinska metiltransferaza, dezoksiribonukleinska (citozin-5-)-metiltransferaza, dezoksiribonukleinska kiselina (citozin-5-)-metiltransferaza, dezoksiribonukleinska kiselina metilaza, dezoksiribonukleinska kiselina metiltransferaza, metilaza modifikacije dezoksiribonukleinske kiseline, dezoksiribonukleinska metilaza, metilfosfotriester-DNK metiltransferaza, modifikaciona metilaza, restrikciono-modifikacioni sistem, site-specifična DNK-metiltransferaza (citozin-specifična), DNK-(citozin ...
Bernard, O; Scheid, M P.; Ripoche, M; and Bennett, D, "Immunological studies of mouse decidual cells. I. Membrane markers of decidual cells in the days after implantation." (1978). Subject Strain Bibliography 1978. 2751 ...
Transcriptomes of about 70,000 single cells from first-trimester deciduas and placentas reveal subsets of perivascular, stromal and natural killer cells in the decidua, according to new research by CTR published on Nature
Mast cells (MCs) are largely known as primary responders in allergic reactions and important cells of the innate immune system. However, recent studies reveal that MCs in fact also play a critical role in the Treg-dependent allograft tolerance by secreting interleukin-9 (IL)-9. In the light of this breaking role for MCs we embarked on a series of studies aiming to analyze whether MCs may be implicated in tolerance towards the semiallogenic fetus growing within the maternal uterus. We confirmed the presence of MCs at the fetal-maternal interface preferentially in maternal decidua (Fig. 1). Their peak is observed around implantation. Fetal rescue by means of antigen-specific Treg was associated with an augmented number of MCs as well as with enhanced expression of MC-related molecules (Tph-1, Mcpt-1 and Mcpt-5) at the fetal-maternal interface and in other organs. Treg treatment was further associated with an increase in the levels of well-known MC growth factors mSCF and IL-3, while IL-9 remained ...
Most of the evidence supporting the sterile womb paradigm was generated with traditional microscopy or culture-based techniques, which are today considered deficient for assessing a microbiome. Researchers have therefore turned to molecular approaches, such as next-generation sequencing, and recent studies have produced evidence (summarized in Table 1) that challenges the sterile womb paradigm. Reports employing these techniques propose that bacteria are not only present within the fetal environment in healthy full-term pregnancies [8, 9, 37, 38], but that they also constitute a placental microbiome that jump-starts the colonization of the fetus [8-10] as part of its normal developmental process [39]. Additionally, it has been suggested that the uterus contains its own microbiome that can contribute to fetal colonization, as the placenta develops from both fetal trophoblasts and maternal decidua (the inner lining of the uterus) [40, 41].. After the early studies on the meconium discussed above, ...
indeciduate definition: indeciduous; Having no decidua; nondeciduate.; Indeciduous.; Having no decidua; nondeciduate.; perhaps not deciduate, as a placenta: applied and to those placental animals when…
nondeciduate definition: described as the lack of a decidua; indeciduate.; described as the absence of a decidua; indeciduate.; just like indeciduate.
|i|Background:|/i| Following implantation, endometrial stroma is transformed into decidual tissue via a complex remodeling process. In parallel with that process, a significant increase in
Previously, we have shown by in situ hybridization that the expression of IGFBP-4, a potential IGF-I inhibitor, correlates to the expression pattern described
Prvobitna analiza DNK kromosoma Y, koja se, kao i mitohondrijska DNK, nasljeđuje od samo jednog roditelja, bila je dosljedna modelu nedavnog afričkog pretka. Međutim, mitohondrijski i podaci sa Y kromosoma nisu se mogli objasniti istom ekspanzijom modernog čovjeka iz Afrike; ekspanzija Y kromosoma uključivala bi genetsko miješanje koje bi zadržalo regionalno lokalne mitohondrijske linije. Uz to, podaci s Y kromosoma ukazali su na kasnije širenje natrag u Afriku iz Azije, pokazavši da genski tok među regijama nije bio jednosmjeran.[81] Rana analiza 15 nekodirajućih dijelova kromosoma X otkrila je dodatne nepodudarnosti s hipotezom nedavnog afričkog pretka. Ta analiza pronašla je višemodalnu distribuciju vremena koalescencije do najmlađeg zajedničkog pretka za te segmente DNK, u suprotnosti s očekivanjima za nedavnu afričku zamjenu; bilo je više vremena koalescencije prije gotovo 2 milijuna godina nego što se očekivalo, što ukazuje na to da se jedna drevna populacija odvojila ...
Nukleosóm je pri evkariotih enota v organizaciji kromatina, ki obsega približno 146 baznih parov DNK, ki je 1,67-krat tesno ovita okrog histonskega oktamera, torej osmih histonskih molekul (po dve kopiji histonov H2A, H2B, H3, H4).[1]. Nukleosome povezuje povezovalna DNK dolžine do 80 baznih parov vezana na zunanji histon H1. Nukleosomi omogočajo strnjenost celotne dednine v jedru, ki meri približno 10 μm. Več nukleosomov se povezuje v kromosom. Nukleosomi so nosilci epigenetskih informacij v obliki kovalentnih modifikacij pripadajočih jedrnih histonov. Niso statične, temveč dinamične strukture. Vezavo transkripcijskih faktorjev na posamezna mesta na DNK lahko facilitirajo ali zavirajo.. ...
R.T.Gergova, M.P.Slavova, V.I.Boev, M.S.Mourdjeva, L.K.Yotova, R.T.Georgieva-Nikolova Comparative investigation of the feasibility of bacterial biofilms formation on the surface of the hybrid material UREASIL. Bulgarian Chemical Communications, 47, 2, 706-713, 2015.. ISSN: 0324-1130IF: 0.349. Elena Stoyanova, M. Mourdjeva, S. Kyurjchiev. Early selection of human fibroblast-derived induced pluripotent stem cells. Biotechnology & Biotechnological Equipment, 29, 5, 942-948, 2015.. ISSN: 1310-2818IF:0.3. Kyurkchiev D, E.Naydenov, K.Tumangelova-Yuzeir, E.Ivanova-Todorova, K.Belemezova, I.Bochev, K.Minkin, M.Mourdjeva, T.Velikova, S.Nachev, S.Kyurkchiev Cells Isolated from Human Glioblastoma Multiforme Express Progesterone-Induced Blocking Factor (PIBF). Cellular and Molecular Neurobiology, 34, 1, 479-489, 2014. ISSN: 0272-4340 IF: 2.506. K.Belemezova, I.Bochev, M.Mourdjeva, A.Shterev, S.Kyurkchiev Effect of GNRH analogues ganirelix and triptorelin on human endometrial stromal cell decidualization in ...
Granularity is a characteristic of both dNK and CD56dim pNK cells, both of which are also called large granular lymphocytes (65), whereas CD56bright pNK cells are known to be less granular (15). Granzyme B and perforin (components of cytotoxic granules) were expressed at similar levels in dNK and CD56dim pNK cells, whereas granzyme A was 3- and 10-fold overexpressed in dNK cells versus the CD56dim and CD56bright pNK cells, respectively (Fig. 3 C). Thus, the potential cytolytic function of dNK cells, probably regulated, cannot be ignored. This nascent activity could be manifest in the decidua under special circumstances (e.g., virus infection of the trophoblast or decidual remodeling).. Many other genes overexpressed in dNK cells that are presented in Fig. 3 (e.g., CD31, CD38, MIP-1 α/β, Syk, LEF1, AARS, arp2/3 subunit, and tubulins) may be of considerable interest but are beyond the scope of the present paper. Likewise, genes that are absent or have lower expression in dNK cells in relation to ...
Elevated level of free 8-iso-prostaglandin F2alpha in the decidua basalis of women with preeclampsia. Increased contents of phospholipids, cholesterol, and lipid peroxides in decidua basalis in women with preeclampsia
The part of the chorion that is in contact with the decidua capsularis undergoes atrophy, so that by the fourth month scarcely a trace of the villi is left. This part of the chorion becomes smooth, and is named the chorion laeve (from the Latin word levis, meaning smooth). As it takes no share in the formation of the placenta, this is also named the non-placental part of the chorion. As the chorion grows, the chorion laeve comes in contact with the decidua parietalis and these layers fuse. The villi at the embryonic pole, which is in contact with the decidua basalis, increase greatly in size and complexity, and hence this part is named the chorion frondosum. Thus the placenta develops from the chorion frondosum and the decidua basalis. ...
Looking for online definition of Decidual cells in the Medical Dictionary? Decidual cells explanation free. What is Decidual cells? Meaning of Decidual cells medical term. What does Decidual cells mean?
Tryptophan 2,3-dioxygenase (T do 2) is a rate-limiting enzyme which directs the conversion of tryptophan to kynurenine. The aim of this study was to examine the expression and regulation of T do 2 in mouse uterus during decidualization. T do 2 mRNA was mainly expressed in the decidua on days 6-8 of pregnancy. By real-time PCR, a high level of T do 2 expression was observed in the uteri from days 6 to 8 of pregnancy, although T do 2 expression was observed on days 1-8. Simultaneously, T do 2 mRNA was also detected under in vivo and in vitro artificial decidualization. Estrogen, progesterone, and 8-bromoadenosine-cAMP could induce the expression of T do 2 in the ovariectomized mouse uterus and uterine stromal cells. T do 2 could regulate cell proliferation and stimulate the expression of decidual marker Dtprp in the uterine stromal cells and decidual cells. Overexpression of T do 2 could upregulate the expression of Ahr, Cox2, and Vegf genes in uterine stromal cells, while T do 2 inhibitor 680C91 ...
Decidualization is a process that results in significant changes to cells of the endometrium in preparation for, and during, pregnancy. This includes morphological and functional changes to endometrial stromal cells (ESCs), the presence of decidual white blood cells (leukocytes), and vascular changes to maternal arteries. The sum of these changes results in the endometrium changing into a structure called the decidua. In humans, the decidua is shed during the third phase of birth. Decidualization plays an important role in promoting placenta formation between a mother and her fetus by mediating the invasiveness of trophoblast cells. It also triggers the production of cellular and molecular factors that result in structural changes, or remodeling, of maternal spiral arteries. Decidualization is required in some mammalian species where embryo implantation and trophoblast cell invasion of the endometrium occurs, also known as hemochorial placentation. This allows maternal blood to come into direct ...
Nancy P, Tagliani E, Tay CS, Asp P, Levy DE, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012 Jun 8;336(6086):1317-21. PubMed PMID: 22679098 ...
Light and electron microscopic studies of the human placental bed suggest that the invasion of trophoblast into decidua depends in some measure on interaction with decidual extracellular matrix (Boyd...
Author(s): Kim, Minah; Park, Hyeung Ju; Seol, Jae Won; Jang, Jeon Yeob; Cho, Young-Suk; Kim, Kyu Rae; Choi, Youngsok; Lydon, John P; Demayo, Francesco J; Shibuya, Masabumi; Ferrara, Napoleone; Sung, Hoon-Ki; Nagy, Andras; Alitalo, Kari; Koh, Gou Young | Abstract: The features and regulation of uterine angiogenesis and vascular remodelling during pregnancy are poorly defined. Here we show that dynamic and variable decidual angiogenesis (sprouting, intussusception and networking), and active vigorous vascular remodelling such as enlargement and elongation of vascular sinus folding (VSF) and mural cell drop-out occur distinctly in a spatiotemporal manner in the rapidly growing mouse uterus during early pregnancy - just after implantation but before placentation. Decidual angiogenesis is mainly regulated through VEGF-A secreted from the progesterone receptor (PR)-expressing decidual stromal cells which are largely distributed in the anti-mesometrial region (AMR). In comparison, P4 -PR-regulated VEGF-A
Professor Graham Burton. The role of the endometrial glands during human early pregnancy has largely been ignored once implantation is complete. However, we have recently demonstrated that the glands are an important source of nutrients for the conceptus during the first trimester. The glandular secretions also contain a rich mix of growth factors and cytokines, and so play a role in regulating early placental development, modulating trophoblast proliferation and differentiation. In other species, such as the sheep and rabbit, there is good evidence that the secretions are essential for promoting growth of the conceptus. It is also known that in these species signals from the conceptus increase the production of glandular secretions to meet its demands. Our interests now are to identify how endometrial gland secretions are regulated in human early pregnancy, their composition, and their effects on trophoblast behaviour. The project may tackle one or more of these topics depending on the ...
Preeclampsia is a severe complication that occurs in the third trimester of pregnancy, affecting around 8% of pregnant women. If it is not diagnosed on time, it may lead to significant complications with both the mother and fetus. Until now, most research on the topic had focused on investigating defects in placenta formation that resulted in the onset of the disease, ignoring the role of the maternal uterine tissue where the embryo is implanted.. Our study, first author of which is Dr. Tamara Garrido-Gómez, shows the link between the onset of preeclampsia and a defect in the decidualization process in the endometrium. Decidualization is an endometrial process that entails preparing the uterus for the implantation of the embryo and a successful pregnancy. This defect not only occurs in the endometrium of women who have suffered from preeclampsia during a previous pregnancy, but also in patients who have preeclampsia at the time of birth.. This discovery offers an explanation as to why women ...
European Larch (Larix decidua) tree is a deciduous conifer but often treated as a honorary broadleaf to provide diversity in conifer plantations. The needles turn red then yellow in autumn before falling off.
talk contribs‎ 443 bytes +443‎ Created page with ==Introduction== --~~~~ Currently Template page only. {{Template:Placenta Links}} ==References== ,references/, ===Reviews=== ===Articles=== ===Search PubMed=== Searc… ...
Sonia S. Hassan is the author of this article in the Journal of Visualized Experiments: Isolation of Leukocytes from the Human Maternal-fetal Interface
Molekulsko kloniranje je skup eksperimentalnih metoda molekularne biologije koji ise koriste za formiranje molekula rekombinantne DNK i za usmeravanje njene replikacije unutar organizma domaćina.[1] Reč kloniranje se koristi jer metod vrši replikaciju jednog molekula DNK počevši od jedne ćelije i generiše veliku popolaciju ćelija koje sadrže identične DNK molekule. Molekularno kloniranje generalno koristi DNK sekvence dva različita organizma: vrste koja je izvor DNK koja se klonira i vrste koja služi kao živi domaćin za replikaciju rekombinantne DNK. Metodi molekularnog kloniranja su centralni za mnoge savremene oblasti biologije i medicine.[2] U konvencionalnom eksperimentu molekulskog kloniranja, DNK koja se klonira se dobija iz željenog organizma, i zatim se tretira sa enzimima u epruveti da bi se formirali manji fragmenti. Naknadno se ti fragmenti kombinuju sa DNK vektorom da bi se formirali rekombinantni DNK molekuli. Oni se zatim uvode u organizam domaćina (tipično u ...
Endometriosis is a common gynaecological disorder characterised by heterotopic islands of uterine mucosa found in many locations, mainly in the pelvis.
The actions of relaxin are highly species specific. The role of relaxin in human endometrial function has not been well understood because of the paucity of in vivo studies in women or in suitable primate models. A model of early human pregnancy was
U molekularnoj biologiji i biohemiji, reverzna transkriptaza (RNK zavisna DNK polimeraza) je DNK polimerazni enzim koji transkribuje jednolančanu RNK u jednolančanu DNK. Ona je isto tako DNK zavisna DNK polimeraza koja sintetiše drugi lanac DNK komplementaran sa reverzno transkribovanom jednolačanom cDNK nakon degradacije originalne iRNK. Ovaj enzim se naziva reverznim zato što normalna transkripcija obuhvata sintezu RNK iz DNK. Dobro izučene reverzne transkriptaze su: ...
The voltaic cell reaction NiO2(s)+4H^+(aq)+ 2Ag(s)Ni^2+(aq)+2H20(l)+ 2Ag^+ has a E cell= +2.48V. What will the cell potential be at a pH of 5.00 when the concentration of NiO^2+ and Ag^+??? I need to know how to set this type of problem up to solve it ...
Invasion of the trophoblast into the maternal decidua is regulated by both the trophoectoderm and the endometrial stroma, and entails the action of tissue remodeling enzymes. Trophoblast invasion requires the action of metalloproteinases (MMPs) to degrade extracellular matrix (ECM) proteins and in turn, decidual cells express tissue inhibitors of MMPs (TIMPs). The balance between these promoting and restraining factors is a key event for the successful outcome of pregnancy. Gene expression is post-transcriptionally regulated by histone deacetylases (HDACs) that unpacks condensed chromatin activating gene expression. In this study we analyze the effect of histone acetylation on the expression of tissue remodeling enzymes and activity of human endometrial stromal cells (hESCs) related to trophoblast invasion control. Treatment of hESCs with the HDAC inhibitor trichostatin A (TSA) increased the expression of TIMP-1 and TIMP-3 while decreased MMP-2, MMP-9 and uPA and have an inhibitory effect on trophoblast
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Large granular lymphocytes (LGL) are the most abundant cell type in first trimester human pregnancy decidua. We have shown previously that CD56-positive decidual LGL have cytotoxic activity against the natural killer (NK) target K562, and that this cytotoxicity is augmented by pretreatment with interleukin-2 (IL-2). We now report that flow cytometrically purified populations of CD56-positive decidual LGL have no cytotoxic activity against either the BeWo choriocarcinoma cell line or freshly isolated term trophoblast. Incubation of unfractionated decidual cells with IL-2 induced cytotoxicity against BeWo, but term trophoblast remained resistant to lysis. Both BeWo and trophoblast showed much lower binding frequencies to decidual or peripheral blood cells than K56 targets, and excess trophoblast did not inhibit cytotoxic activity against K562. This suggests that the resistance of trophoblast to lysis by either decidual or peripheral blood LGL is due to the lack of accessible NK target structures on the
Prolactin production was induced in HESCs in response to 8-br-cAMP and P. Dihydrotestosterone further enhanced the secretion of PRL in cells treated with 8-br-cAMP plus P. The effect of DHT was blocked by the antiandrogen flutamide. Dihydrotestosterone enhanced resistance to oxidative stress-induced apoptosis on decidualized HESCs. Moreover, DHT enhanced FOXO1 expression in parallel with increased SOD2 protein but not with SOD1.. Conclusion(s): ...
Decidualization of the endometrial stromal cells (ESC), considered to be stimulated by progesterone and/or cAMP, is crucial for embryo implantation and placentation. In this study, we isolated a novel clone encoding decidual protein induced by progesterone (Depp) from a human ESC cDNA library enrich …
Hormone-regulated proliferation and differentiation of endometrial stromal cells (ESCs) determine overall endometrial plasticity and receptivity to embryos. Previously we revealed that ESCs may undergo premature senescence, accompanied by proliferation loss and various intracellular alterations. Here we focused on whether and how senescence may be transmitted within the ESCs population. We revealed that senescent ESCs may induce paracrine senescence in young counterparts via cell contacts, secreted factors and extracellular vesicles. According to secretome-wide profiling we identified plasminogen activator inhibitor -1 (PAI-1) to be the most prominent protein secreted by senescent ESCs (data are available via ProteomeXchange with identifier PXD015742). By applying CRISPR/Cas9 techniques we disclosed that PAI-1 secreted by senescent ESCs may serve as the master-regulator of paracrine senescence progression within the ESCs population. Unraveled molecular basis of senescence transduction in the ESCs