Probable ATP Dependent RNA Helicase DDX58 (DEAD Box Protein 58 or RIG I Like Receptor 1 or Retinoic Acid Inducible Gene 1 Protein or DDX58 or EC 3.6.4.13) - Pipeline
Looking for online definition of Melanoma differentiation-associated protein 6 in the Medical Dictionary? Melanoma differentiation-associated protein 6 explanation free. What is Melanoma differentiation-associated protein 6? Meaning of Melanoma differentiation-associated protein 6 medical term. What does Melanoma differentiation-associated protein 6 mean?
DExD/H-box proteins are a diverse class of proteins that are implicated in RNA and RNP remodeling. They have sequence homology to DNA helicases and share conserved ATPase domains, suggesting that they use the energy of ATP binding and hydrolysis to mediate conformational rearrangements in RNAs. In the past, the action of DExD/H-box proteins has been characterized primarily on simple model substrates such as small RNA duplexes. It is not known how DExD/H-box proteins manipulate structured RNA, what determines target specificity and what molecular events follow their action. Here, using the well-characterized Tetrahymena group I intron ribozyme, I performed kinetic and thermodynamic studies to understand the mechanism of CYT-19 and related DExD/Hbox proteins. CYT-19 has been shown previously to facilitate the folding of several group I and group II introns. I demonstrated that CYT-19 acts as a chaperone, accelerating the re-folding of a long-lived misfolded species of the Tetrahymena group I ...
We have previously demonstrated that the DEAD-box RNA helicase p68 is an important regulator of gene expression [1, 2], whilst other groups have shown that p68 interacts with and coactivates estrogen receptor alpha (ERα) [3, 4]. The main focus of our project is to investigate the molecular mechanism of ERα coactivation by p68 and to examine the potential consequences for breast cancer development.. We have established that the interaction of p68 and ERα requires the DNA binding domain of ERα and the C terminus of p68. Importantly, this region of p68 lies outside the conserved helicase core and was previously shown by us to be essential for transcriptional regulation by p68. Additionally, coactivation of ERα by p68 requires the ligand binding/AF2 region of ERα and is consistent with the model that p68 is recruited to ERα-responsive promoters in response to estrogen [4]. We have also shown that p72, a helicase that is very highly related to p68 and that had previously been suggested to act ...
The recessive nuclear vdl (for variegated and distorted leaf) mutant of tobacco was obtained by T-DNA insertion and characterized by variegated leaves and abnormal roots and flowers. Affected leaf tissues were white and distorted, lacked palisadic cells, and contained undifferentiated plastids. The variegation was due to phenotypic, rather than genetic, instability. Genomic and cDNA clones were obtained for both the mutant and wild-type VDL alleles. Three transcripts, resulting from alternate intron splicing or polyadenylation, were found for the wild type. The transcripts potentially encode a set of proteins (53, 19, and 15 kD) sharing the same N-terminal region that contains a chloroplast transit peptide capable of importing the green fluorescent protein into chloroplasts. The predicted 53-kD product belongs to the DEAD box RNA helicase family. In the homozygous vdl mutant, T-DNA insertion resulted in accumulation of the shortest transcript and the absence of the RNA helicase-encoding ...
Ribosome biogenesis is a highly complex process that requires several cofactors, including DExD/H-box RNA helicases (RHs). RHs are a family of ATPases that rearrange the secondary structures of RNA and thus remodel ribonucleoprotein (RNP) complexes. DExD/H-box RHs are found in most organisms and play critical roles in a variety of RNA-involved cellular events. In human and yeast cells, many DExD/H box RHs participate in multiple steps of ribosome biogenesis and regulate cellular proliferation and stress responses. In plants, several DExD/H-box RHs have been demonstrated to be associated with plant development and abiotic and biotic stress tolerance through their functions in modulating pre-rRNA processing. In this review, we summarize the pleiotropic roles of DExD/H-box RHs in rRNA biogenesis and other biological functions. We also describe the overall function of the DExD/H-box RH family in ribosome biogenesis based on data from human and yeast.
Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5-single-stranded DNA overhangs. Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs. Involved in transcriptional regulation. Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner. Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter. Involved in regulation of translation initiation. Proposed to be involved in positive regulation of translation such as of cyclin E1/CCNE1 mRNA and specifically of mRNAs containing complex secondary structures in their 5UTRs; these functions seem to require RNA helicase activity. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The role of ARF in regulating p53 is well established, but the mechanisms by which it exerts its p53-independent tumor suppressor function are yet to be fully characterized. Our group and others have recently shown the regulation of translation by ARF, but mechanistic details of its involvement are limited. Both mouse and human ARF interact with nucleolar proteins involved in ribosome biogenesis as well as ribosomal components themselves (1, 38). Furthermore, ectopic expression of human p14ARF decreases polyribosomes in a p53-independent manner (38). ARF has recently been linked to ribosome biogenesis through its regulation of TTF-1 (29) and its ability to inhibit ribosome export via its nucleolar interaction with NPM (15, 38). Here we have shown that ARF can control the protein composition of the nucleolus, the central organelle in ribosome biogenesis. Our observation that ARF can regulate DDX5 RNA helicase provides a mechanistic explanation for the inhibitory effects of ARF on 47S rRNA ...
RNA helicases are vital components of various pathways for post-transcriptional gene regulation. TgHoDI is a newly identified member of the DEAD-box RNA helicase family in Toxoplasma gondii. This study was aimed at characterizing the function of TgHoDI in post-transcriptional regulation during various stages of the parasite life cycle. Through immunofluorescence analyses, TgHoDI was found to colocalize with mRNA aggregates in cytoplasmic foci. Furthermore, colocalization studies of TgHoDI with PABPC3 identified these proteins as components of RNA aggregates known as stress granules. Cycloheximide and arsenite treatment of extracellular parasites revealed the presence of mechanisms involved in the determination of mRNA fate in Toxoplasma. Also, TgHoDI was found to functionally compliment the temperature sensitive phenotype in Δdhh1 yeast, validating its role in stress response. These findings confirm that TgHoDI is a vital component in the formation of stress granules and possibly involved in the
医学分子生物学 国家重点实验室 分子生物学 基础医学 重大疾病 北京协和医学院 中国医学科学院 基础医学研究所
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ATP-dependent RNA helicase p54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD), DEAD box protein 6, EC 3.6.4.13, EC 3.6.1, FLJ36338, HLR2DEAD box-6, Oncogene RCK, probable ATP-dependent RNA helicase DDX6, ...
Introduction: The DEAD box RNA helicase DDX3X (DDX3) has been demonstrated to have a pro-oncogenic role in breast cancer. Thus, abrogating DDX3 activity was explored as a viable anti-cancer strategy using a small molecule inhibitor of DDX3, referred to as RK-33. RK-33 was found to inhibit non-homologous end joining (NHEJ), a DNA double strand break (DSB) repair mechanism. Therefore, we set out to determine the utility of RK-33 in breast cancers of BRCA1 mutation carriers, as they exhibit deficiencies in homologous recombination, an additional DSB repair mechanism. We hypothesized that BRCA1-deficient breast cancers would be more dependent on NHEJ to maintain genomic stability and thus inhibiting DDX3 activity by RK-33 would be an efficacious treatment strategy.. Methods: We evaluated DDX3 protein expression levels by immunohistochemistry in 102 BRCA1 and 29 BRCA2 germline mutation carriers and compared these to those of 345 sporadic breast cancer patients. In addition, DDX3 expression in two ...
Bacterial and viral RNA are potent stimulators of the innate immune system, leading to immune cell activation and type I IFN production (Takeuchi and Akira, 2010). Generally, RNA recognition takes place in the endosome or cytoplasm and is mediated by Toll-like receptors (TLRs) and retinoic acid inducible gene I (RIG-I)-like helicases, respectively. In more detail, TLR3 recognizes double-stranded viral RNA and mRNA, whereas TLR7 and TLR8 sense viral or bacterial single-stranded RNA and short interfering RNA (siRNA; Blasius and Beutler, 2010). In contrast, cytoplasmic detection of viral and bacterial RNA is mediated by the RNA helicases RIG-I and MDA5 (melanoma differentiation-associated gene 5; Kato et al., 2006; Monroe et al., 2009).. Of note, RNA modifications in ribosomal RNA and transfer RNA (tRNA) such as 2′-O-methylation, base methylation (e.g., m5C, m6A, and m5U), and the occurrence of pseudouridine negatively modify the immunostimulatory potential of synthetic RNA (Karikó et al., ...
ATP-dependent RNA helicase DDX39 is an enzyme that in humans is encoded by the DDX39 gene. This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. GRCh38: Ensembl release 89: ENSG00000123136 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000005481 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Peelman LJ, Chardon P, Nunes M, Renard C, Geffrotin C, Vaiman M, Van Zeveren A, Coppieters W, van de Weghe A, Bouquet Y, et al. (Aug 1995). "The BAT1 gene in the MHC encodes ...
Buy DBP8 recombinant protein, ATP-dependent RNA helicase DBP8 (DBP8) Recombinant Protein-XP_001525308.1 (MBS1038466) product datasheet at MyBioSource, Recombinant Proteins
RIG-1 antibody for detecting human probable ATP-dependent RNA helicase DDX58. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
K06269 PPP1C; serine/threonine-protein phosphatase PP1 catalytic subunit [EC:3.1.3.16] K06269 PPP1C; serine/threonine-protein phosphatase PP1 catalytic subunit [EC:3.1.3.16] K06269 PPP1C; serine/threonine-protein phosphatase PP1 catalytic subunit [EC:3.1.3.16] K16833 PPP1R2; protein phosphatase inhibitor 2 K17550 PPP1R7; protein phosphatase 1 regulatory subunit 7 K17553 PPP1R11; protein phosphatase 1 regulatory subunit 11 K17560 URI1; unconventional prefoldin RPB5 interactor 1 K14806 DDX31; ATP-dependent RNA helicase DDX31/DBP7 [EC:3.6.4.13] K13099 CD2BP2; CD2 antigen cytoplasmic tail-binding protein 2 K11498 CENPE; centromeric protein E K03238 EIF2S2; translation initiation factor 2 subunit 2 K03504 POLD3; DNA polymerase delta subunit 3 K11407 HDAC6; histone deacetylase 6 [EC:3.5.1.98] K01251 E3.3.1.1; adenosylhomocysteinase [EC:3.3.1.1] K11446 KDM5; [histone H3]-trimethyl-L-lysine4 demethylase [EC:1.14.11.67] K02932 RP-L5e; large subunit ribosomal protein L5e K02210 MCM7; DNA replication ...
Long non-coding RNAs (lncRNAs) are a class of non-protein-coding RNA molecules, which are involved in various biological processes, including chromatin modification, cell differentiation, pre-mRNA transcription and splicing, protein translation, etc. During the last decade, increasing evidence has suggested the involvement of lncRNAs in both immune and antiviral responses as positive or negative regulators. The immunity-associated lncRNAs modulate diverse and multilayered immune checkpoints, including activation or repression of innate immune signaling components, such as interleukin (IL)-8, IL-10, retinoic acid inducible gene I, toll-like receptors 1, 3, and 8, and interferon (IFN) regulatory factor 7, transcriptional regulation of various IFN-stimulated genes, and initiation of the cell apoptosis pathways ...
Innate immunity is characterized by production of type I interferon which is necessary for the stimulation of effective anti-viral host defense. Upon recognition of cytosol viral dsRNA species, RIG-I-Like Receptors (RLRs), as well as many co-regulators, are recruited to adaptor protein IPS-1 and trigger innate immune responses. FADD (Fas associated with death domain) and RIP1 (receptor-interacting protein 1), have been reported to be recruited to this IPS-1 complex during viral infection and essential for optimal RLR signaling. Here we reported a novel type I interferon inducible DExD/H family helicase DDX24, which was found and confirmed to specifically associate with FADD through yeast two hybrid system and co-immunoprecipitation. Overexpression of DDX24 negatively regulates dsRNA induced type I IFNs signaling, while knockdown of DDX24 by siRNA has the opposite effect. Moreover, Plaque assays of virus titer consistently demonstrate that DDX24 also negatively regulates the cellular antiviral response.
Sigma-Aldrich offers abstracts and full-text articles by [Kislay Parvatiyar, Zhiqiang Zhang, Rosane M Teles, Songying Ouyang, Yan Jiang, Shankar S Iyer, Shivam A Zaver, Mirjam Schenk, Shang Zeng, Wenwan Zhong, Zhi-Jie Liu, Robert L Modlin, Yong-jun Liu, Genhong Cheng].
Pertschy B., Schneider C., Gnadig M., Schafer T., Tollervey D., Hurt E.. Many RNA nucleases and helicases participate in ribosome biogenesis, but how they cooperate with each other is largely unknown. Here we report that in vivo cleavage of the yeast pre-rRNA at site D, the 3-end of the 18 S rRNA, requires functional interactions between PIN (PilT N terminus) domain protein Nob1 and the DEAH box RNA helicase Prp43. Nob1 showed specific cleavage on a D-site substrate analogue in vitro, which was abolished by mutations in the Nob1 PIN domain or the RNA substrate. Genetic analyses linked Nob1 to the late pre-40 S-associated factor Ltv1, the RNA helicase Prp43, and its cofactor Pfa1. In strains lacking Ltv1, mutation of Prp43 or Pfa1 led to a striking accumulation of 20 S pre-rRNA in the cytoplasm due to inhibition of site D cleavage. This phenotype was suppressed by increased dosage of wild-type Nob1 but not by Nob1 variants mutated in the catalytic site. In ltv1/pfa1 mutants the 20 S pre-rRNA was ...
Upon recognition of viral components by pattern recognition receptors, including TLRs and retinoic acid-inducible gene I (RIG-I)- like helicases, cells are activated to produce type I IFN and proinflammatory cytokines. These pathways are tightly regulated by host to prevent inappropriate cellular response, but viruses can down-regulate these pathways for their survival. Recently, identification of negative regulators for cytoplasmic RNA-mediated antiviral signaling, especially the RIG-I pathway, attract much attention. However, there is no report about negative regulation of RIG-I antiviral pathway by microRNAs (miRNA) to date. We found that vesicular stomatitis virus (VSV) infection up-regulated miR-146a expression in mouse macrophages in TLR-myeloid differentiation factor 88-independent but RIG-I-NF-κB-dependent manner. In turn, miR-146a negatively regulated VSV-triggered type I IFN production, thus promoting VSV replication in macrophages. In addition to two known miR-146a targets, TRAF6 and ...
Recognition of invading viruses by the host is elicited by cellular sensors which trigger signaling cascades that lead to type I interferon (IFN) gene expression. Retinoic acid-inducible gene I (RIG-I) has emerged as a key receptor for the detection of viral RNA in the cytosol, inducing IFN-mediated innate immune responses to limit viral replication through its interaction with MAVS (also called IPS-1, CARDIF, or VISA). Upon the recognition of viral RNA, the Lys-172 residue of RIG-I undergoes ubiquitination induced by tripartite motif protein 25 (TRIM25), an essential protein for antiviral signal transduction. Here we demonstrate that phosphorylation represents another regulatory mechanism for RIG-I-mediated antiviral activity. Using protein purification and mass spectrometry analysis, we identified three phosphorylation sites in the amino-terminal caspase recruitment domains (CARDs) of RIG-I. One of these residues, Thr-170, is located in close proximity to Lys-172, and we speculated that its ...
Interferon induced with helicase C domain 1 (IFIH1) protein is a member of a group of RNA helicases, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), known as DEAD box proteins. IFIH1 is also known as melanoma differentiation-associated protein 5 (MDA5), clinically amyopathic dermatomyositis autoantigen 140 kDa, helicase with 2 CARD domains (HLCD), helicard, murabutide down-regulated protein, RIG-I-like receptor 2 (RLR-2), CADM-140 autoantigen, RNA helicase-DEAD box protein 116 (RH116), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, and IDDM19. IFIH1/MDA5 plays a major role in sensing viral infection and in the activation of a cascade of antiviral and inflammatory responses. IFIH1 gene expression is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Treatment with both these agents causes irreversible reprogramming of melanomas, while treatment with either agent alone only achieves reversible differentiation. Mutations in ...
Interferon induced with helicase C domain 1 (IFIH1) protein is a member of a group of RNA helicases, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), known as DEAD box proteins. IFIH1 is also known as melanoma differentiation-associated protein 5 (MDA5), clinically amyopathic dermatomyositis autoantigen 140 kDa, helicase with 2 CARD domains (HLCD), helicard, murabutide down-regulated protein, RIG-I-like receptor 2 (RLR-2), CADM-140 autoantigen, RNA helicase-DEAD box protein 116 (RH116), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, and IDDM19. IFIH1/MDA5 plays a major role in sensing viral infection and in the activation of a cascade of antiviral and inflammatory responses. IFIH1 gene expression is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Treatment with both these agents causes irreversible reprogramming of melanomas, while treatment with either agent alone only achieves reversible differentiation. Mutations in ...
Mammalian cells possess multiple sensors for recognition of invasion by a broad range of microbes. This recognition occurs through specific molecular signatures found across various pathogens. Toll-like receptors (TLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) and C-type lectin receptors (CLRs) are the major cellular pathogen-recognition receptors (PRRs) responsible for this recognition. TLRs are transmembrane sensors, whereas other PRRs mainly localize in the cytoplasm for the activation of type I interferons and pro-inflammatory cytokines. Among these PRRs, RLRs are well known for their indispensable role in sensing the invasion of RNA viruses. This review summarizes recent advances in knowledge about viral recognition by RLRs and their signalling pathways, and introduces newly emerging RNA helicases involved in innate immune responses.. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Translational control is primarily exerted at the initiation phase, a complex process mediated by a number of eucaryotic translation initiation factors (eIFs), during which ribosomes are recruited to the 5′ end of an mRNA and positioned at a start codon (reviewed in Gingras et al, 1999b; Hershey and Merrick, 2000). The mRNA 5′ end is distinguished by the presence of a cap structure (m7GpppN, where m is a methyl group and N is any nucleotide), which is specifically bound by the cap‐binding protein eIF4E. eIF4E, via an interaction with the large scaffolding protein eIF4G, guides the translation machinery to the 5′ end of mRNA. eIF4E and eIF4G function as components of the dynamic, heterotrimeric eIF4F complex, along with the DEAD box RNA helicase eIF4A. Optimal ribosome binding is thought to require a region of single‐stranded mRNA (Gingras et al, 1999b). Thus, it has been proposed that one critical function of eIF4F is to melt cap‐proximal inhibitory secondary structure to provide a ...
Specific families of pattern recognition receptors are responsible for detecting viral pathogens and generating innate immune responses. Non-self RNA appearing in a cell as a result of intracellular viral replication is recognized by a family of cytosolic RNA helicases termed RIG-I-like receptors (RLRs). The RLR proteins include RIG-I, MDA5, and LGP2 and are expressed in both immune and nonimmune cells. Upon recognition of viral nucleic acids, RLRs recruit specific intracellular adaptor proteins to initiate signaling pathways that lead to the synthesis of type I interferon and other inflammatory cytokines, which are important for eliminating viruses ...
Complete information for DDX20 gene (Protein Coding), DEAD-Box Helicase 20, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
We are using single molecule fluorescence techniques to monitor movements of molecular motors moving on RNA. First, I will present our recent finding that a cytosolic viral RNA sensor RIG-I (Retinoic acid-Inducible Gene 1) is a translocase on double stran
Frankfurt - Paul Hastings LLP, a leading global law firm, announced today that it is advising biopharmaceutical company MSD (tradename of Merck & Co., Inc., Kenilworth, N.J., USA (NYSE: MRK)) on its acquisition of Munich-headquartered therapeutics company Rigontec.. Under the terms of the agreement, MSD will make an upfront cash payment of €115 million to Rigontecs shareholders; based on the attainment of certain clinical, development, regulatory and commercial milestones, MSD may make additional contingent payments of up to €349 million. The transaction is subject to certain closing conditions.. Rigontec is a pioneer in accessing the retinoic acid-inducible gene I (RIG-I) pathway, part of the innate immune system, as a novel and distinct approach in cancer immunotherapy to induce both immediate and long-term anti-tumor immunity. Rigontecs lead candidate, RGT100, is currently in Phase I development evaluating treatment in patients with various tumors.. Rigontec was founded in 2014 as a ...
DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked (DDX3X) is a gene that encodes a protein that belongs to the large DEAD-box protein family. The protein functions in the in the nucleus and the cytoplasm of the cell and exhibits ATP-dependent RNA helicase activity and RNA-independent ATPase activity. Missense mutations, nonsense mutations, silent mutations, and frameshift deletions are observed in cancers such as endometrial cancer, skin cancer, and stomach cancer.. DDX3X is altered in 0.14% of all cancers. The most common alterations in DDX3X are DDX3X D506G (0.00%) and DDX3X Mutation (0.00%) [3]. ...
Cells react to viral infections by exhibiting innate immune responses. Central to the host innate antiviral responses is production of type I IFN, which is regulated by members of the IFN regulatory factor (IRF) family of transcription factors (1-7). Among the nine members in mammalian cells, two closely related ones, IRF3 and IRF7, have been implicated as the main regulators of type I IFN gene expression elicited by viruses (2, 4, 8-10). Although IRF3 is expressed ubiquitously and constitutively, IRF7 is expressed at low levels in most cells, but its expression is upregulated by viral infections. Despite low expression, through a positive feedback loop, IRF7 plays a dominant role in regulation of IFN induction, as evidenced by the abrogation of IFN production in most cell types of Irf7−/− but not in Irf3−/− mice (8, 9).. Host cells sense viral infection with pathogen recognition receptors such as membrane-bound TLRs, cytosolic retinoic acid-inducible gene I (RIG-I)-like receptors ...
Genome-wide association studies (GWASs) are regularly used to map genomic regions contributing to common human diseases, but they often do not identify the precise causative genes and sequence variants. To identify causative type 1 diabetes (T1D) variants, we resequenced exons and splice sites of 10 candidate genes in pools of DNA from 480 patients and 480 controls and tested their disease association in over 30,000 participants. We discovered four rare variants that lowered T1D risk independently of each other (odds ratio = 0.51 to 0.74; P = 1.3 x 10(-3) to 2.1 x 10(-16)) in IFIH1 (interferon induced with helicase C domain 1), a gene located in a region previously associated with T1D by GWASs. These variants are predicted to alter the expression and structure of IFIH1 [MDA5 (melanoma differentiation-associated protein 5)], a cytoplasmic helicase that mediates induction of interferon response to viral RNA. This finding firmly establishes the role of IFIH1 in T1D and demonstrates that resequencing
Genome-wide association studies (GWASs) are regularly used to map genomic regions contributing to common human diseases, but they often do not identify the precise causative genes and sequence variants. To identify causative type 1 diabetes (T1D) variants, we resequenced exons and splice sites of 10 candidate genes in pools of DNA from 480 patients and 480 controls and tested their disease association in over 30,000 participants. We discovered four rare variants that lowered T1D risk independently of each other (odds ratio = 0.51 to 0.74; P = 1.3 x 10(-3) to 2.1 x 10(-16)) in IFIH1 (interferon induced with helicase C domain 1), a gene located in a region previously associated with T1D by GWASs. These variants are predicted to alter the expression and structure of IFIH1 [MDA5 (melanoma differentiation-associated protein 5)], a cytoplasmic helicase that mediates induction of interferon response to viral RNA. This finding firmly establishes the role of IFIH1 in T1D and demonstrates that resequencing
Adults older than 65 account for most of the deaths caused by respiratory influenza A virus (IAV) infections, but the underlying mechanisms for this susceptibility are poorly understood. IAV RNA is detected by the cytosolic sensor retinoic acid-inducible gene I (RIG-I), which induces the production of type I interferons (IFNs) that curtail the spread of the virus and promote the elimination of infected cells. We have previously identified a marked defect in the IAV-inducible secretion of type I IFNs, but not proinflammatory cytokines, in monocytes from older (,65 years) healthy human donors. We found that monocytes from older adults exhibited decreased abundance of the adaptor protein TRAF3 (tumor necrosis factor receptor-associated factor 3) because of its increased proteasomal degradation with age, thereby impairing the primary RIG-I signaling pathway for the induction of type I IFNs. We determined that monocytes from older adults also failed to effectively stimulate the production of the IFN ...
Next, we tested whether Dhh1 tethering to FBA1 mRNA was sufficient to localize FBA1 mRNA to PBs. However, we were unable to detect any enrichment of FBA1 mRNA in Dcp2-GPP-labeled PBs in a wild-type strain background (unpublished data). Because Dhh1 tethering leads to a drastic reduction of steady-state FBA1 mRNA levels (Fig. 1), we reasoned that FBA1 mRNA turnover might be too rapid to observe PB accumulation or, alternatively, that FBA1 mRNA levels are potentially too low to be visualized by in situ hybridization. Therefore, we monitored the localization of FBA1 mRNA in xrn1Δ cells. In this background, FBA1-PP7 mRNA levels are restored, but tethering Dhh1 to FBA1 still results in translational repression (Fig. 2). Whereas deleting XRN1 caused the constitutive formation of Dcp2-containing PBs (Fig. 3 B; Teixeira and Parker, 2007), we found that FBA1 mRNA tethered to the PP7CP alone was enriched in only ∼5% of the Dcp2-labeled PBs (Fig. 3 B). In contrast, tethering Dhh1-PP7CP to FBA1 mRNA ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
ID ECOAB_1_PE1561 STANDARD; PRT; 457 AA. AC ECOAB_1_PE1561; E1P8F7; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=ATP-independent RNA helicase DbpA; (ECOAB_1.PE1561). GN Name=dbpA; OrderedLocusNames=ECABU_c16270; OS ESCHERICHIA COLI ABU 83972. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=655817; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ECOAB_1.PE1561. CC Escherichia coli OR:K5:H- (strain ABU 83972) chromosome, complete CC sequence. CC -!- ANNOTATIONS ORIGIN:E1P8F7_ECOAB CC -!- SIMILARITY: Belongs to the DEAD box helicase family. CC -!- GENE_FAMILY: HOG000268809 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; E1P8F7; -. DR EMBL; CP001671; ADN46180.1; -; Genomic_DNA. DR GenomeReviews; CP001671_GR; ECABU_c16270. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008026; ...
Abnova Human DDX3X Partial ORF (NP_076829.1, 1 a.a. - 55 a.a.) Recombinant Protein with GST-tag at N-terminal 25µg Life Sciences:Protein Biology:Proteins:Proteins A-Z:Proteins
References for Abcams Recombinant Human DDX17 protein (ab112392). Please let us know if you have used this product in your publication
FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) ...
To ensure that there wasnt something going on at another step in the path, some other things were tried: A silencing RNA strand was introduced into the cell that would stop the production of RIG-I and MAVS. No IFN-β was produced. DNASE-I is an enzyme that breaks down DNA. When that was introduced, no IFN-β was produced. On the other hand, IFN-β was produced in the presence of RNASE-I, so breaking down RNA had no effect. ...
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Principal Investigator:YONEYAMA Mitsutoshi, Project Period (FY):2008 - 2012, Research Category:Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Project Area:Diversity and asymmetry achieved by RNA program
For some infections, such as those caused by some viruses, fever is a normal part of the infection fighting process. But what induces the fever? One possibility is a protein called RIG-1 (retinoic acid inducible gene protein one). This protein is a helicase which recognizes double stranded RNA (dsRNA). dsRNA is often found in viruses but not in uninfected animal cells. This ensures that RIG-1 is only active in infected cells. ...
Complete information for DDX17 gene (Protein Coding), DEAD-Box Helicase 17, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium