BioAssay record AID 397811 submitted by ChEMBL: Antiviral activity against HIV infected in human MOLT4 cells assessed as inhibition of virus-induced cytopathic effect.
TY - JOUR. T1 - Recurrence incidence in follow-up of patients affected by condylomatosis of the uterine cervix with VCE (viral cytopathic effect). AU - Alberico, S.. AU - Facca, M. C.. AU - Mandruzzato, G. P.. AU - Di Bonito, L.. AU - Colautti, I.. AU - Patriarca, S.. PY - 1985. Y1 - 1985. UR - http://www.scopus.com/inward/record.url?scp=0021850423&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0021850423&partnerID=8YFLogxK. M3 - Article. C2 - 4054152. AN - SCOPUS:0021850423. VL - 6. SP - 222. EP - 226. JO - European Journal of Gynaecological Oncology. JF - European Journal of Gynaecological Oncology. SN - 0392-2936. IS - 3. ER - ...
Counihan, Natalie, Daniel, LM, Chojnacki, J and Anderson, DA 2006, Infrared fluorescent immunofocus assay (IR-FIFA) for the quantitation of non-cytopathic and minimally cytopathic viruses., Journal of Virological Methods, vol. 133, no. 1, pp. 62-69, doi: 10.1016/j.jviromet.2005.10.023. ...
BioAssay record AID 336244 submitted by ChEMBL: Antiviral activity against 10-3 MOI HSV1 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect.
Cytopathic effect or cytopathogenic effect (abbreviated CPE) refers to structural changes in host cells that are caused by viral invasion. The infecting virus causes lysis of the host cell or when the cell dies without lysis due to an inability to reproduce. Both of these effects occur due to CPEs. If a virus causes these morphological changes in the host cell, it is said to be cytopathogenic. Common examples of CPE include rounding of the infected cell, fusion with adjacent cells to form syncytia, and the appearance of nuclear or cytoplasmic inclusion bodies. CPEs and other changes in cell morphology are only a few of the many effects by cytocidal viruses. When a cytocidal virus infects a permissive cell, the viruses kill the host cell through changes in cell morphology, in cell physiology, and the biosynthetic events that follow. These changes are necessary for efficient virus replication but at the expense of the host cell. CPEs are important aspects of a viral infection in diagnostics. Many ...
A novel electronic cell sensor array technology, the real-time cell analysis (RTCA) system, was developed to monitor cell events. Unlike the conventional methods labeling the target cells with fluorescence, luminescence, or light absorption, the RTCA system allows for label-free detection of cell processes directly without the incorporation of labels. Here, we used this new format to measure the cytopathic effect (CPE) of the 2009 influenza A (H1N1) virus and the efficacy of neutralizing antibodies in human sera to this virus. The real-time dynamic monitoring of CPE was performed on MDCK cell cultures infected with the H1N1 virus, ranging from 5.50×10(2) to 5.50×10(7) copies/mL. The resulting CPE kinetic curves were automatically recorded and were both time and viral load dependent. The CPE kinetics were also distinguishable between different H1N1 stains, as the onset of CPE induced by the A/Shanghai/37T/2009 H1N1 virus was earlier than that of the A/Shanghai/143T/2009 H1N1 virus. Furthermore, ...
Discussion:. Human adenovirus is a linear, non-enveloped, double-stranded DNA virus that typically causes mild respiratory, gastrointestinal, and conjunctival illnesses in healthy persons. In kidney transplant recipients, urinary tract infection is the most frequently reported manifestation. The most frequent signs and symptoms at presentation include dysuria, fever, hematuria, sterile pyuria and acute kidney injury.. A renal allograft biopsy is essential to differentiate adenoviral nephritis from that of acute rejection or other pathology. Typical light microscopic findings in adenovirus nephropathy include tubular cell necrosis (with tubular basement membrane rupture) associated with severe interstitial inflammation (sometimes with granuloma formation), focal interstitial hemorrhage and viral cytopathic changes including peripheral condensed chromatin, basophilic nuclear inclusions and nuclear enlargement. In some cases such as ours, viral inclusions are not identified, presumably because of ...
Titles in this series - The subspecialty of Cytopathology is 60 years old and has become established as a solid and reliable discipline in medicine. As expected, cytopathology literature has expanded in a remarkably short period of ...
This article describes how the Viral ToxGlo™ Assay can be used to expedite the discovery of new chemical compounds directed against cytolytic viruses, while also providing insight into potential off-target toxicities.
Cytopathology is the use of specialist diagnostic techniques to examine individual cells extracted from tissues to determine the cause and nature of a disease.
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We analyzed the env genes of cytopathic and noncytopathic biological clones derived from two HIV-1-infected children with discordant clinical courses. Chimeric viruses were constructed by switching env regions from V2 through V3 of the biological clones with the corresponding region from the molecular clone NL4-3. These HIV-1 chimeric viruses exhibited similar replication kinetics as well as syncytium-inducing abilities. The chimeric virus containing the env region of noncytopathic biological clone, GC6 8-4, was noncytopathic in an in vitro cell-killing assay, while the chimeric virus containing the env region of cytopathic biological clone, HC4, was cytopathic in the in vitro cell-killing assay. These studies suggest the presence of a cytopathicity determinant that maps to the envelope sequences contained within the downstream region of V2 and within the V3 region (nucleotide position 6822 to nucleotide position 7250, based on NL4-3 sequence).
A cell system was developed for the reproducible detection of human T-lymphotropic retroviruses (HTLV family) from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS). The cells are specific clones from a permissive human neoplastic T-cell line. Some of the clones permanently grow and continuously produce large amounts of virus after infection with cytopathic (HTLV-III) variants of these viruses. One cytopathic effect of HTLV-III in this system is the arrangement of multiple nuclei in a characteristic ring formation in giant cells of the infected T-cell population. These structures can be used as an indicator to detect HTLV-III in clinical specimens. This system opens the way to the routine detection of HTLV-III and related cytopathic variants of HTLV in patients with AIDS or pre-AIDS and in healthy carriers, and it provides large amounts of virus for detailed molecular and immunological analyses. ...
Please follow the policy for the processing of specimens for Cytopathology as outlined in the Specimen Submission Manual document "Processing of Specimens for Cytopathology" (PDF attached for download).. Download PDF: Processing of Specimens for Cytopathology 2011. Safety: Universal precautions are followed.. Contact: ...
The xCELLigence Real-time Cell Analyzer system perfectly integrates micro electronics and cell biology and has a number of advantages in comparison to conventional cell analysis systems. It is suitable for uninterrupted monitoring of processes in living cells and for monitoring real-time kinetics. The system shows excellent sensitivity and reproducibility when monitoring the overall cell population of a cell culture.
The Cytopathology department of The Johns Hopkins University School of Medicine and The Office of Medical Informatics have created this site to enhance the teaching program at the School of Medicine in regards to the Cytopathology course.
The Cytopathology department of The Johns Hopkins University School of Medicine and The Office of Medical Informatics have created this site to enhance the teaching program at the School of Medicine in regards to the Cytopathology course.
The course is organized as a theoretical-practical review of relevant aspects of cytopathology.. The course is designed to be very interactive. The faculty will give lectures, show typical morphologic entities and discuss how to approach specific issues in multiheaded microscopes (two 16 headed microscopes and 2 microscopes with projection screens), dividing the attendants into 4 different groups. The attendants will rotate through the different settings, so everyone has similar exposure to the invited faculty, cases and settings. The course is limited to 90 participants in order to accommodate everybody at the practical sessions. An option to attend the lectures only is also available with a lower registration fee and limited to 125 individuals plus. ...
Pris: 633,-. E-bok, 2012. Leveres direkte via nedlastning . Kjøp boken Serous Cavity Fluid and Cerebrospinal Fluid Cytopathology av Syed Z. Ali, Edmund S. Cibas (ISBN 9781461417767) hos Adlibris.com. Fri frakt.
I was wondering if anyone knows of a place I can find a complete (better), full description of the new pathology codes for the Cytopathology FISH test
This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e
Looking for online definition of enteric cytopathogenic human orphan virus in the Medical Dictionary? enteric cytopathogenic human orphan virus explanation free. What is enteric cytopathogenic human orphan virus? Meaning of enteric cytopathogenic human orphan virus medical term. What does enteric cytopathogenic human orphan virus mean?
Human enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. Presently, no vaccines or antiviral drugs have been clinically available to employ against EV71. In this study, we demonstrate that treatment with chebulagic acid reduced the viral cytopathic effect on rhabdomyosarcoma cells with an IC50 of 12.5 μg/mL. The utilization of the chebulagic acid treatment on mice challenged with a lethal dose of enterovirus 71 was able to efficiently reduce mortality and relieve clinical symptoms through the inhibition of viral replication. Chebulagic acid may represent a potential therapeutic agent to control infections to enterovirus 71.
Bovine parainfluenza-3 virus was found to replicate in alveolar macrophages in vitro. Cytopathic changes include spindle cell and giant cell formation, cellular detachment, and diffuse cell lysis progressing to total destruction of the monolayer. Direct immunofluorescent microscopy demonstrated viral antigens in greater than 90% of the cells three days after virus inoculation. Virus titers in culture supernatants increased by 6 a factor of 1.6 X 10 TCID50 by six days post inoculation. Phagocytosis assays showed no statistical differences of glass adherent cells in virus infected and control macrophages. When the number of glass adherent cells available for assay are taken into account (normalization) differences in phagocytic values are significant at 5 and 7 days post inoculation. Phagosomelysosome fusion assays demonstrated marked reduction of fusion activity of virus infected macrophages compared to control macrophages. Fusion values of infected cells were 51% and 53% that of control cells at days 4
The Essentials in Cytopathology book series fulfills the need for an easy-to-use and authoritative synopsis of site specific topics in cytopathology. These guide books fit into the lab coat pocket and
Foot-and-mouth disease (FMD) is a highly contagious disease of domestic and wild ruminants that is caused by FMD virus (FMDV). FMD outbreaks have occurred in livestock-containing regions worldwide. Apigenin, which is a flavonoid naturally existing in plant, possesses various pharmacological effects, including anti-inflammatory, anticancer, antioxidant and antiviral activities. Results show that apigenin can inhibit FMDV-mediated cytopathogenic effect and FMDV replication in vitro. Further studies demonstrate the following: (i) apigenin inhibits FMDV infection at the viral post-entry stage; (ii) apigenin does not exhibit direct extracellular virucidal activity; and (iii) apigenin interferes with the translational activity of FMDV driven by internal ribosome entry site. Studies on applying apigein in vivo are required for drug development and further identification of potential drug targets against FDMV infection.
Fusion of the BCL9 HD2 domain to E1A increases the cytopathic effect of an oncolytic adenovirus that targets colon cancer cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Khaled Abdel-Fattah R. El-Sabban working at Professor and Consultant of Histopathology and Cytopathology,College of Medicine, Ta..
A rhabdovirus associated with a lethal hemorrhagic disease in cultured turbot Scophthalm us maximus Linnaeus was isolated. The virus induced typical cytopathogenic effects (CPE) in 9 of 15 fish cell lines examined and was then propagated and isolated from infected carp leucocyte cells (CLC). Electron microscopy observations revealed that the negatively stained virions had a typical bullet-shaped morphology with one rounded end and one flat base end. The bullet-shaped morphology was more obvious and clear in ultrathin sections of infected cells. Experimental infections also indicated that the S. maximus rhabdovirus (SMRV) was not only a viral pathogen for cultured turbot, but also had the ability to infect other fish species, such as freshwater grass carp. A partial nucleotide sequence of the SMRV polymerase gene was determined by RT-PCR using 2 pairs of degenerate primers designed according to the conserved sequences of rhabdovirus polymerase genes. Homology analysis, amino acid sequence ...
TY - JOUR. T1 - Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture.. AU - Hirano, N.. AU - Goto, N.. AU - Makino, Shinji. AU - Fujiwara, K.. PY - 1981. Y1 - 1981. N2 - After inoculation with JHM strain into DBT cell monolayers, a persistently infected DBT cell culture was established without producing typical cytopathic changes after about 15th passages. By immunofluorescence virus specific antigen was demonstrated in 10 to 15% DBT cells. This persistently infected culture (JHM-CC) was resistant to superinfection with parental JHM, but such resistance was not shown against vesicular stomatitis virus. JHM-CC virus produced small plaques on DBT cell monolayers. Temperature sensitive (TS) mutant, defective interfering (DI) particle or interferon was not detected in the JHM-CC. To intracerebral inoculation with JHM-CC virus, cortisone treated ICR mice survived without showing clinical signs, however, demyelinating lesions were produced in the brain and spinal cord of ...
Cases Low-Grade Papillary Urothelial Tumor and Herpes Simplex Infection (Ed Eutman) Synopsis cytopathogenic effect of HSV multinucleated (...)
In: Proceedings of a symposium on continuous cell lines as substrates for biologicals, Arlington, Virginia USA, May 1988. Basel: Karger, 1989;113-24 (Developments in biological standardization; 70 ...
The ASCs diverse membership includes representatives from other countries who share a vision of education, research and continuous improvement in the standards and quality of patient care. The ASC is a unique society that provides a forum where physicians and cytotechnologists can interact and network with each other on both a personal and professional level ...
The ASC?s diverse membership includes representatives from other countries who share a vision of education, research and continuous improvement in the standards and quality of patient care. The ASC is a unique society that provides a forum where physicians and cytotechnologists can interact and network with each other on both a personal and professional level ...
The ASCs diverse membership includes representatives from other countries who share a vision of education, research and continuous improvement in the standards and quality of patient care. The ASC is a unique society that provides a forum where physicians and cytotechnologists can interact and network with each other on both a personal and professional level ...
Dr. Azar Daneshbod, MD is a Cytopathology Specialist in Los Alamitos, CA. Be sure to call ahead with Dr. Daneshbod to book an appointment.
American Society of Cytopathology (ASC) - Find your next career at CytoJobs. Check back frequently as new jobs are posted every day.
Contents Babies from Birth to Age Vaccines give parents the power... 2 Vaccines are recommended throughout our lives... 3 Talk to your doctor... 3 Vaccines are very safe... 3 Whooping Cough (Pertussis)...
The similarity in the intracellular infection of macrophages and protozoa by L. pneumophila is quite remarkable. Within both evolutionarily distant host cells, the bacterium multiplies within a phagosome that does not fuse with lysosomes and is surrounded by mitochondria and the RER (1, 2, 12, 26, 27, 41). Many mutants of L. pneumophila (including dot andicm insertion mutants) exhibit similar defective phenotypes in cytopathogenicity and intracellular replication within both macrophages and protozoa (7, 16, 23). These observations suggest that L. pneumophila utilizes many similar, yet unknown, mechanisms to survive and replicate within both evolutionarily distant hosts.. In this study, we have examined the possibility that L. pneumophila differentially utilizes certain loci to replicate within macrophages but not protozoa. We have identified 32 mutants that exhibit different degrees of defects in cytopathogenicity, intracellular survival, and replication within mammalian macrophages but exhibit ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Two Research Awards are offered by the Papanicolaou Society for Cytopathology to pathologists-in-training for abstracts presented at the Annual Meeting of the United States and Canadian Academy of Pathology.. The abstracts are evaluated for the novelty of the idea presented and the scientific and/or practical value of the topic. Winners are selected by members of the Research Committee.. The awards are presented at the Papanicolaou Society of Cytopathology Companion Meeting of the United States and Canadian Academy of Pathology.. To Qualify for the Award:. ...
methane-producing bacteria might survive beneath the surface of Mars, and identification of a cytopathogenic toxin in a bacterium associated with preterm birth
Maresca M; Mahfoud R; Garmy N; et al. (2003). „The virotoxin model of HIV-1 enteropathy: involvement of GPR15/Bob and galactosylceramide in the cytopathic effects induced by HIV-1 gp120 in the HT-29-D4 intestinal cell line.". J. Biomed. Sci. 10 (1): 156-66. PMID 12566994. doi:10.1159/000068089 ...
세포배양 및 Bioprocessing 유체에 흔히 오염되는 Mollicutes과 Mycoplasma는 현재까지 190여종이 알려져 있으며, 자가복제가 가능한 현존하는 가장 작은 생명체입니다. 0.15-0.3 µm 작은 size로 일반적인 filtration을 통과하며, Peptidoglycan 형성 억제 기전을 활용하는 많은 종류의 박테리아 항생제에 반응하지 않아 그 오염빈도가 매우 높습니다. 또한 광학 현미경상으로 관찰이 불가하며, viral, bacteria, fungal infection시 나타나는 cytopathic effect, 배양액의 pH 변화, 배양액의 혼탁도(turbid) 변화등과 같은 2차적 변화를 거의 유발하지 않기 때문에 그 감염 여부를 인지하기 어렵습니다 ...
Visit Healthgrades for information on Dr. Charles Gadol, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
LG dLite vs Sanyo Vero by Kyocera. Compare the specs and features of the LG dLite and Sanyo Vero by Kyocera to see which is better.
Biology Assignment Help, Bovine viral diarrhoea, Bovine viral diarrhoea Bovine viral diarrhoea (BVD) and mucosal disease (MD) are clinically dissimilar disease syndrome yet have a common viral etiology. The acute disease is called as BVD. The term mucosal disease is reserved for chronic diseas
Knowing how to identify cattle tetanus is vital for long term health of your herd. Come online to Dairy Australia today for all the latest information.
The outcomes of a voluntary bovine viral diarrhoea virus (BVDV) control programme that has been in place in the Netherlands since 1997 were analysed. This BVDV-free programme was studied in dairy herds in the period 1 August 2007 to 1 August 2013. The programme was based on a test and cull approach at the herd level, after which the BVDV status was monitored by testing young stock for antibodies ...
IMSTAR developed the unique Pathfinder™ technology for optimized multimodal scanning, cell imaging & analysis, to assist health researchers...