TY - JOUR. T1 - Different antibody response to a neutralizing epitope of human cytomegalovirus glycoprotein B among seropositive individuals. AU - Ayata, Minoru. AU - Sugano, Tohru. AU - Murayama, Tsugiya. AU - Sakamuro, Daitoku. AU - Takegami, Tsutomu. AU - Matsumoto, Yoh‐Ichi ‐I. AU - Furukawa, Toru. PY - 1994/8. Y1 - 1994/8. N2 - The amino‐terminal portion of human cytomeg‐alovirus glycoprotein B (HCMV‐gB) was expressed as a fusion protein to analyze the neutralizing epitope recognized by human monoclonal antibody C23 and the humoral immune response to this epitope. The linear neutralizing epitope was further localized to the pep‐tide within 17 amino acids (position 68‐84) which were conserved between two HCMV laboratory strains. Ten out of 17 HCMV‐seropositive human sera contained the antibody against this epitope. Although seven sera were negative for reacting with the fusion protein, the viruses isolated from the same patients retained the epitope. The immunogenicity of the ...
TY - JOUR. T1 - Monitoring of ganciclovir sensitivity of multiple human cytomegalovirus strains coinfecting blood of an AIDS patient by an immediate-early antigen plaque assay. AU - Gerna, Giuseppe. AU - Baldanti, Fausto. AU - Zavattoni, Maurizio. AU - Sarasini, Antonella. AU - Percivalle, Elena. AU - Revello, M. Grazia. PY - 1992/10/1. Y1 - 1992/10/1. N2 - A plaque-reduction assay for chemosensitivity testing of human cytomegalovirus (HCMV) strains was developed based on early detection of viral plaques 96 h p.i. by a monoclonal antibody to the major immediate-early protein p72. Sequential HCMV isolates from an AIDS patient undergoing multiple courses of ganciclovir treatment during an 18-month follow-up were tested by the new assay, showing emergence of a ganciclovir-resistant strain. However, cloning of viral isolates and Southern blot hybridization analysis showed the simultaneous presence of three different HCMV strains in blood. Of these, the resistant strain was likely to be selected ...
In immunocompromised patients, human cytomegalovirus (HCMV) infection can lead to severe, life-threatening diseases, such as pneumonitis, hepatitis, gastrointestinal tract disease, and retinitis. We previously reported that a 70% ethanol extract of Elaeocarpus sylvestris leaves (ESE) inhibits human cytomegalovirus (HCMV) replication in vitro. In the present study, we determined the solvent fraction of ESE that inhibits HCMV replication using activity-guided fractionation. Activity-guided fractionation of ESE was performed to determine the solvent fraction that inhibits HCMV replication. Effects of solvent fractions on HCMV lytic gene expression and major immediate-early (MIE) enhancer/promoter activity were further investigated. Among the solvent fractions tested, the EtOAc fraction of ESE markedly reduced HCMV lytic gene expression and viral replication in vitro without exerting significant cytotoxic effects against human foreskin fibroblasts (HFF). Furthermore, the EtOAc fraction negatively affected
HCMV is the leading infectious cause of mental retardation and deafness in infants with congenital HCMV infection. Primary HCMV infections during pregnancy carry the highest risk of fetal infection and disease. No intervention of proven efficacy is available in case of primary HCMV infection in pregnancy. However, a study published in 2005 (Nigro et al., NEJM 353:1350-62, 2005) reported that in pregnant women with primary HCMV infection treated with HCMV-specific hyperimmune globulin (Cytotect®, Biotest) the risk of transmitting the infection to the fetus was reduced from 40% to 16%. Unfortunately, since the study was conducted with inadequate controls, the actual efficacy of hyperimmune globulin could not be properly assessed.. In the present randomized, double-blind, placebo-controlled, multicenter trial pregnant women with ascertained primary HCMV infection at 4-26 weeks of gestation will be randomized to receive Cytotect® or placebo intravenously within 6 weeks after the presumed onset of ...
Background & Objectives: Interaction of cytomegalovirus glycoprotein B with toll-like receptors of dendritic cells leads to early signaling and innate immune responses. The aim of this study is to evaluate the effects of cytomegalovirus glycoprotein B on the maturation and function of monocyte-derived dendritic cells in treated groups in comparison with control ...
Human cytomegalovirus (HCMV) manipulates many aspects of host cell biology to create an intracellular milieu optimally supportive of its replication and spread. Our study reveals that levels of several components of the purinergic signaling system, including the P2Y2 and P2X5 receptors, are elevated in HCMV-infected fibroblasts. Knockdown and drug treatment experiments demonstrated that P2Y2 enhances the yield of virus, whereas P2X5 reduces HCMV production. The HCMV IE1 protein induces P2Y2 expression; and P2Y2-mediated signaling is important for efficient HCMV gene expression, DNA synthesis, and the production of infectious HCMV progeny. P2Y2 cooperates with the viral UL37x1 protein to regulate cystolic Ca2+ levels. P2Y2 also regulates PI3K/Akt signaling and infected cell motility. Thus, P2Y2 functions at multiple points within the viral replication cycle to support the efficient production of HCMV progeny, and it may facilitate in vivo viral spread through its role in cell migration. ...
TY - JOUR. T1 - Phosphorothioate-modified oligodeoxynucleotides inhibit human cytomegalovirus replication by blocking virus entry. AU - Luganini, Anna. AU - Caposio, Patrizia. AU - Landolfo, Santo. AU - Gribaudo, Giorgio. PY - 2008/3. Y1 - 2008/3. N2 - Studies in animal models have provided evidence that Toll-like receptor 9 (TLR9) agonists, such as synthetic oligodeoxynucleotides (ODNs) that contain immunostimulatory deoxycytidyl-deoxyguanosine (CpG) motifs (CpG ODNs), protect against a wide range of viral pathogens. This antiviral activity has been suggested to be indirect and secondary to CpG-induced cytokines and inflammatory responses triggered through TLR9 activation. However, few studies have addressed the potential of CpG ODNs as direct antiviral agents. Here, we report on the ability of some CpG ODNs to directly suppress, almost completely, human cytomegalovirus (HCMV) replication in both primary fibroblasts and endothelial cells. Murine CMV replication was inhibited as well, whereas no ...
Original Article. Congenital cytomegalovirus infection refers to a condition where cytomegalovirus is transmitted in the prenatal period. Ster, B.... Cytomegalovirus (CMV) is a member of the Herpesviridae family, along with herpes simplex viruses 1 and 2, Epstein Barr virus, and varicella zoster virus. Vid W. Chronic inflammation may be a causative factor in a variety of cancers. Mberlin, M. Review. Congenital cytomegalovirus infection refers to a condition where cytomegalovirus is transmitted in the prenatal period. General, the longer the inflammation persists, the higher the risk of cancer. Systematic review of publications indexed in the PubMed database was performed for HSCT studies. General, the longer the inflammation persists, the higher the risk of cancer. 8ajg. Otein Losing Enteropathy: Case Illustrations and. Ablo J. J Gastroenterol 2010; 105:4349; doi:10. Chronic inflammation may be a causative factor in a variety of cancers. Lganciclovir for Symptomatic Congenital Cytomegalovirus ...
TY - JOUR. T1 - Human cytomegalovirus UL18 utilizes US6 for evading the NK and T-cell responses. AU - Kim, Youngkyun. AU - Park, Boyoun. AU - Cho, Sunglim. AU - Shin, Jinwook. AU - Cho, Kwangmin. AU - Jun, Youngsoo. AU - Ahn, Kwangseog. PY - 2008/8/1. Y1 - 2008/8/1. N2 - Human cytomegalovirus (HCMV) US6 glycoprotein inhibits TAP function, resulting in down-regulation of MHC class I molecules at the cell surface. Cells lacking MHC class I molecules are susceptible to NK cell lysis. HCMV expresses UL18, a MHC class I homolog that functions as a surrogate to prevent host cell lysis. Despite a high level of sequence and structural homology between UL18 and MHC class I molecules, surface expression of MHC class I, but not UL18, is down regulated by US6. Here, we describe a mechanism of action by which HCMV UL18 avoids attack by the self-derived TAP inhibitor US6. UL18 abrogates US6 inhibition of ATP binding by TAP and, thereby, restores TAP-mediated peptide translocation. In addition, UL18 together ...
Life-threatening opportunistic cytomegalovirus infection is a complication of the acquired immunodeficiency syndrome (AIDS) that occurs in 7.4% or more of patients with AIDS. Cytomegalovirus retinitis, colitis, esophagitis, and gastritis are the commonest manifestations of severe cytomegalovirus end-organ disease. Extensive trials with intravenous ganciclovir, a nucleoside analogue with myelosuppressive toxicity, have shown that ganciclovir halts the progression of cytomegalovirus retinitis and gastrointestinal disease. Since relapse is common when therapy is discontinued, most patients with AIDS need lifelong maintenance therapy. The clinical response to ganciclovir therapy is usually accompanied by diminished shedding of the virus. Based on limited data, foscarnet, a pyrophosphate analogue, also appears to have some efficacy in treating cytomegalovirus infection. Unlike ganciclovir, foscarnet does not cause myelosuppression. An important direction for future clinical research is the ...
TY - JOUR. T1 - Detection of cytomegalovirus genomes in human skin fibroblasts by DNA hybridization. AU - Williams, L. L.. AU - Blakeslee, J. R.. AU - Boldogh, Istvan. AU - Huang, E. S.. PY - 1980. Y1 - 1980. N2 - A previous isolation of a human cytomegalovirus (CMV) from fibroblasts derived from intact skin of a Charcot-Marie-Tooth disease patient has prompted examination of six blind-coded cultured human skin lines by CMV DNA hybridization. The detection of CMV genome equivalents in three of the lines suggests that, in some cases, intact human skin may be a site of CMV latency.. AB - A previous isolation of a human cytomegalovirus (CMV) from fibroblasts derived from intact skin of a Charcot-Marie-Tooth disease patient has prompted examination of six blind-coded cultured human skin lines by CMV DNA hybridization. The detection of CMV genome equivalents in three of the lines suggests that, in some cases, intact human skin may be a site of CMV latency.. UR - ...
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Human cytomegalovirus (HCMV) is a frequent cause of major disease following primary infection or reactivation from latency in immunocompromised patients. Infection of non-permissive mononuclear cells is used for analyses of HCMV latency in vitro. Using this approach, it is shown here that repression of lytic gene expression following experimental infection of CD34+ cells, a site of HCMV latency in vivo, correlates with recruitment of repressive chromatin around the major immediate-early promoter (MIEP). Furthermore, long-term culture of CD34+ cells results in carriage of viral genomes in which the MIEP remains associated with transcriptionally repressive chromatin. Finally, specific differentiation of long-term cultures of infected CD34+ cells to mature dendritic cells results in acetylation of histones bound to the MIEP, concomitant loss of heterochromatin protein 1 and the reactivation of HCMV. These data are consistent with ex vivo analyses of latency and may provide a model for further analyses of
persons body, it may or may not result in the active disease. Once in the body, such virus can be dormant for many years, but becomes active and can result in the disease at any time.. Between 60 and 90% of adults have experienced Cytomegalovirus infection at one point of their lives; although, these people experience no symptoms. A severe infection usually happens only in individuals, who have impaired immune systems (for instance, patients with AIDS).. Cytomegalovirus infections before birth can result in stillbirth, miscarriage and may be fatal for newborns. Cytomegalovirus may be life-threatening, if extensive brain or liver damage, anemia, or bleeding, occurs. Many individuals, who get the Cytomegalovirus infection after birth and harbor such a virus, experience no symptoms. However, a healthy individual with the infection can have a fever and feel ill.. If an individual receives blood transfusion consisting of cytomegalovirus, symptoms can start two to four weeks later. Such symptoms ...
The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future ...
Human cytomegalovirus (HCMV) is the most significant microbial cause of birth defects, including brain damage and deafness, in developed nations. There is a compelling argument that a reduction in HCMV load would provide significant benefit in improving human health and reducing health care costs. Vaccination is the most practical way to achieve such a reduction in HCMV load. There are two important clinical settings where vaccination will have a significant impact on health outcome. The first is the prevention of the sequelae of congenital HCMV infection. A prophylactic vaccine to prevent congenital HCMV infection would make a major public health and economic contribution by reducing the incidence of birth defects.. The second setting is the prevention of HCMV-related complications in organ transplantation. HCMV is a major pathogen in both solid organ and bone marrow transplant recipients. We have identified a large number of cytotoxic T cell epitopes from a variety of HCMV antigens. A subset ...
Despite a lot of research, the etiology and progression of breast cancer remain incompletely understood. Recently, human cytomegalovirus (HCMV) was reported as a risk factor for breast cancer. The aim of this study was to know whether breast cancer could be caused by cytomegalovirus or not? In this experiment seventeen samples of RAZI/A mice with spontaneous breast cancer were being gathered from laboratory animals department. Histopathology and polymerase chain reaction (PCR) tests were done on breast tissue samples. Formalin-fixed tissue specimens were obtained from mouse normal breast tissues (n:17) and mouse mammary tumors (n:17). Detection of mouse cytomegalovirus was done by the pUC57-MCK-2 plasmid. Our histopathology data showed Adenocarcinoma type B in mouse with mammary tumors. There was a significant difference between mice with spontaneous breast cancer and control by Pearson Chi-Square (Value: 17.000b and P=0.000). More research will be needed to determine the effect of cytomegalovirus on
TY - JOUR. T1 - Growth of human cytomegalovirus in primary macrophages. AU - Söderberg-Nauclér, Cecilia. AU - Fish, Kenneth N.. AU - Nelson, Jay. PY - 1998/9. Y1 - 1998/9. N2 - Human cytomegalovirus (HCMV) is a major human pathogen that causes considerable disease among immunocompromised individuals. A primary infection results in life-long persistence of the virus in a latent form. HCMV is known to be transferred by blood products, bone marrow, and solid organs, but the cell type that carries the latent infection has been difficult to identify. We have recently demonstrated reactivation of latent HCMV in allogeneically stimulated monocyte-derived macrophages (Allo-MDM). Reactivation occurred only in macrophages produced by allogeneic but not mitogenic stimulation. The presence of dendritic cell markers on some Allo-MDM cells suggested that these macrophages were related to dendritic cells. However, dendritic cells obtained by stimulation of monocytes with interleukin-4 (IL-4) and ...
5E5A: Crystal structure of the chromatin-tethering domain of Human cytomegalovirus IE1 protein bound to the nucleosome core particle
This study was initiated in order to define the relevance of ND10 domains for HCMV replication. Experimental results of previous studies suggested that ND10 domains may play a pivotal role for the initiation of HCMV IE gene expression. Arguments that could be interpreted in favor of such a proviral role of ND10 were as follows: (i) viral genomes were found to be deposited at the periphery of ND10 (29, 31); (ii) several regulatory proteins of HCMV at least transiently colocalize with PML and other ND10 components (2, 26, 32, 37); (iii) an immediate transcript environment consisting of viral IE transcripts, the IE2 protein, and SC35 domains was reported to form adjacent to ND10-localized HCMV genomes, suggesting that this spatial association is critical for the correct initiation of viral gene expression (31); (iv) only ND10-associated viral genomes were shown to develop into viral replication compartments (4, 48).. As an experimental approach to study the role of ND10 for HCMV replication, we ...
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Gene expression during productive infection by the human cytomegalovirus (HCMV) occurs in an ordered and sequential manner, beginning with immediate early (IE), then early (E) and finally late (L) gene expression. Significant work has addressed the regulation of IE and E gene expression while relatively little work has addressed the control of late gene expression. In order to further address HCMV late gene expression, the promoter of the HCMV UL75 (glycoprotein H, gH) late gene was characterized. The data obtained in this study were combined with observations made in two other studies that have addressed HCMV late gene expression to develop a model of the regulation of HCMV late gene expression. The gH promoter and numerous promoter mutants were cloned into a reporter vector to address sequences responsible for the regulation of gene expression. These gH promoter constructs were transfected into human fibroblasts and subsequently infected with HCMV. Our data revealed that viral infection was necessary
Human cytomegalovirus (HCMV)-encoded G protein-coupled-receptor US28 is believed to participate in virus dissemination through modulation of cell migration and immune evasion. US28 binds different CC chemokines and the CX3C chemokine CX3CL1. Membrane-anchored CX3CL1 is expressed by immune-activated endothelial cells, causing redirection of CX3CR1-expressing leukocytes in the blood to sites of infection. Here, we used stable transfected cell lines to examine how US28 expression affects cell migration on immobilized full-length CX3CL1, to model how HCMV-infected leukocytes interact with inflamed endothelium. We observed that US28-expressing cells migrated more than CX3CR1-expressing cells when adhering to immobilized CX3CL1. US28-induced migration was G protein-signalling dependent and was blocked by the phospholipase Cβ inhibitor U73122 and the intracellular calcium chelator BAPTA-AM. In addition, migration was inhibited in a dose-dependent manner by competition from CCL2 and CCL5, whereas CCL3 ...
TY - JOUR. T1 - Refinement in the production and purification of recombinant HCMV IE1-pp65 protein for the generation of epitope-specific T cell immunity. AU - Nguyen, Thi Hoang Oanh. AU - Mifsud, Nicole Andrea. AU - Stewart, Lisbeth A. AU - Rose, Mingus J. AU - Etto, Tamara L. AU - Williamson, Nicholas A. AU - Purcell, Anthony Wayne. AU - Kotsimbos, Tom C. AU - Schwarer, Anthony. PY - 2008. Y1 - 2008. UR - http://www.elsevier.com/locate/yprep. M3 - Article. VL - 61. SP - 22. EP - 30. JO - Protein Expression and Purification. JF - Protein Expression and Purification. SN - 1046-5928. IS - 1. ER - ...
The human cytomegalovirus (hCMV) major immediate-early 1 protein (IE1) is best known for activating transcription to facilitate viral replication. Here we present transcriptome data indicating that IE1 is as significant a repressor as it is an activator of host gene expression. Human cells induced to express IE1 exhibit global repression of IL6- and oncostatin M-responsive STAT3 target genes. This repression is followed by STAT1 phosphorylation and activation of STAT1 target genes normally induced by IFNγ. The observed repression and subsequent activation are both mediated through the same region (amino acids 410 to 445) in the C-terminal domain of IE1, and this region serves as a binding site for STAT3. Depletion of STAT3 phenocopies the STAT1-dependent IFNγ-like response to IE1. In contrast, depletion of the IL6 receptor (IL6ST) or the STAT kinase JAK1 prevents this response. Accordingly, treatment with IL6 leads to prolonged STAT1 instead of STAT3 activation in wild-type IE1 expressing ...
T-0902611 benzenesulfonate is a cytomegalovirus replication inhibitor that may be useful in the treatment of cytomegalovirus infections.
Summary The characteristics of four human cytomegalovirus (HCMV)-specific monoclonal antibodies as assessed by ELISA, immunofluorescence, immunoprecipitation and Western blotting are described. Two antibodies recognized a 67K late polypeptide of HCMV, one recognized 43K and 79K polypeptides present early and late in HCMV-infected cells, and the fourth identified a 72K early nuclear protein of HCMV. The antibodies recognized these antigens in all HCMV isolates tested by immunofluorescence and ELISA, but demonstrated inter-isolate variations in polypeptides recognized by Western blotting.
McCartney, S. A., Brignole, E. J., Kolegraff, K. N., Loveland, A. N., Ussin, L. M., and Gibson, W. Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a. J. Biol. Chem.280:33206-33212 (2005) Loveland, A. N., Chan, C.-K., Brignole, E. J., and Gibson, W. Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity. J. Virol. 79:12961-12968 (2005) Wang, J., Loveland, A. N., Kattenhorn, L. M., Ploegh, H. L., and Gibson, W. High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: Mutant viruses altered in its active-site cysteine or histidine are viable. J. Virol. 80:6003-6012 (2006). Margulies, B. J. and Gibson, W. The chemokine receptor homologue encoded by UL27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles. ...
Human (Homo sapiens)micro-RNAs (hsa-miRNAs) regulate virus and host-gene translation, but the biological impact in patientswith human cytomegalovirus (hCMV) infection is notwell defined in a clinically relevantmodel. First, we compared hsa-miRNA expression profiles in peripheral blood mononuclear cells from 35 transplant recipients with and without CMV viremia by using a microarray chip covering 847hsa-miRNAs. This approach demonstrated a set of 142 differentially expressed hsamiRNAs. Next, we examined the effect of each of these miRNAs on viral growth by using human fibroblasts (human foreskin fibroblast-1) infected with the hCMV Towne strain, identifyinga subset of proviral andantiviral hsa-miRNAs. miRNA-target prediction software indicated potential binding sites within the hCMV genome (e.g., hCMV-UL52 and -UL100 [UL¼unique long]) and host-genes (e.g., interleukin-1 receptor, IRF1). Luciferaseexpressing plasmid constructs and immunoblotting confirmed several predicted miRNA targets. Finally, ...
The pp28 (UL99) gene of human cytomegalovirus is expressed as a true late gene, in that DNA synthesis is absolutely required for mRNA expression. Our previous studies demonstrated that pp28 promoter sequences from position -40 to +106 are sufficient for late gene expression in the context of the viral genome (C. P. Kohler, J. A. Kerry, M. Carter, V. P. Muzithras, T. R. Jones, and R. M. Stenberg, J. Virol. 68:6589-6597, 1994). To extend these studies, we have examined the sequences in the downstream leader region of the pp28 gene for their role in late gene expression. Deletion of sequences from position -6 to +46 (deltaSS) results in a threefold increase in gene expression in transient assays. In contrast, deletion of sequences from position +46 to +88 (deltaA) has little effect on gene expression. These results indicate that the sequences from position -6 to +46 may repress gene expression. To further analyze this region, site-directed mutagenesis was performed. Mutation of residues from either ...
Human cytomegalovirus (CMV) remains a major cause of congenital disease in children as well as a significant opportunistic pathogen in immunocompromised individ...
Human cytomegalovirus (HCMV) was first isolated 50 years ago, when the new technology of cell culture became available. The pathogenesis of HCMV disease is complex, involving contributions from the host as well as from the virus. Increasing knowledge about the genetic composition of the virus can help to illuminate this complex series of relationships and provide a rational basis for therapeutic intervention and prevention of disease. The major immediate-early promoter (MIEP) enhancer contains multiple recognition sites for the transcription factors. Additionally, the MIEP is specifically transactivated by the tegument protein pp71, which is released as soon as incoming virions are uncoated. Thus, HCMV employs multiple methods independent of de novo viral gene expression to induce an intracellular milieu favorable to the initiation of immediate-early (IE) gene transcription. Humoral immunity could reduce the level of HCMV replication and reduce disease without being able to eliminate infection entirely.
TY - JOUR. T1 - Characterizing human cytomegalovirus reinfection in congenitally infected infants. T2 - An evolutionary perspective. AU - Pokalyuk, Cornelia. AU - Renzette, Nicholas. AU - Irwin, Kristen K.. AU - Pfeifer, Susanne. AU - Gibson, Laura. AU - Britt, William J.. AU - Yamamoto, Aparecida Y.. AU - Mussi-Pinhata, Marisa M.. AU - Kowalik, Timothy F.. AU - Jensen, Jeffrey. PY - 2017. Y1 - 2017. N2 - Given the strong selective pressures often faced by populations when colonizing a novel habitat, the level of variation present on which selection may act is an important indicator of adaptive potential. While often discussed in an ecological context, this notion is also highly relevant in our clinical understanding of viral infection, in which the novel habitat is a new host. Thus, quantifying the factors determining levels of variation is of considerable importance for the design of improved treatment strategies. Here, we focus on such a quantification of human cytomegalovirus (HCMV) - a ...
Results:. Using a Cox proportional-hazards model, CMVIG was shown to reduce severe CMV-associated disease (multi-organ CMV disease, CMV pneumonia, or invasive fungal disease associated with CMV infection) from 26% to 12% (relative risk, 0.39; 95% CI, 0.17 to 0.89). When we controlled for the use of monoclonal antibodies to T cells (OKT3), CMVIG use was still protective (relative risk, 0.39; CI, 0.17 to 0.90). Rates of CMV disease were reduced from 31% to 19% (relative risk, 0.56; CI, 0.3 to 1.1) in CMVIG recipients although no effect on rates of CMV infection, graft survival, or patient survival at 1 year were shown. When we controlled for the urgency of transplantation and OKT3 use, a reduction in CMV disease (relative risk, 0.22; CI, 0.06 to 0.81) was shown for globulin recipients for all serologic groups except for the highest risk group (the CMV-seropositive donor, CMV-seronegative group). ...
Define cytomegalovirus: a herpesvirus (species Human herpesvirus 5 of the genus Cytomegalovirus) that in healthy… - cytomegalovirus in a sentence
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TY - JOUR. T1 - Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture. AU - McClintock, J. T.. AU - Thaker, S. R.. AU - Mosher, M.. AU - Jones, D.. AU - Forman, M.. AU - Charache, P.. AU - Wright, K.. AU - Keiser, J.. AU - Taub, F. E.. PY - 1989. Y1 - 1989. N2 - The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.. AB - The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody ...
BACKGROUND:. Despite progress in understanding the pathophysiology of human cytomegalovirus (HCMV) infections, its manifestations in the immune compromised host are frequently associated with high morbidity and mortality. In this setting, HCMV disease can develop e.g. following immune suppression as a result of reactivation of latent HCMV acquired earlier in life. The mechanisms leading to establishment of latent infections and their subsequent reactivation are not clear. It is also unknown whether HCMV exists in a latent form with limited viral gene expression or as a persistent infection with normal virus transcription.. DESIGN NARRATIVE:. The specific aims of the study were to: 1) examine the percentage of HCMV positive donors whose bone marrow progenitors contained HCMV DNA using nested PCR and determine if virus could be rescued from those cells. 2) Analyze the HCMV life cycle in hematopoietic progenitor and stem cells. 3) identify and analyze HCMV gene expression in in vivo infected ...
Learn about the potential side effects of cytomegalovirus immune globulin. Includes common and rare side effects information for consumers and healthcare professionals.
Learn about Cytogam (Cytomegalovirus Immune Globulin Intravenous Human) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit (Catalog No. HCMV100K)includes all the assay kit components except the enzyme. It includes 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.. The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit Plus -100 (Catalog No. HCMV100KE). includes all the assay kit components. It includes 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP, 33 µl of 100 x HCMV DNA polymerase, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA.. ...
Previous studies revealed that HCMV infection induces a persistent reconfiguration of the NK cell compartment characterized by the expansion of an NK cell subset expressing the activating NKG2C receptor. This finding has been demonstrated both in healthy individuals and in patients with different pathological conditions (10-16, 30, 31). Remarkably, in patients undergoing UCBT, HCMV infection/reactivation markedly influenced NK cell reconstitution, promoting the rapid appearance and expansion of NKG2C+KIR+NKG2A−Siglec-7− NK cells (17, 18). These studies suggested that the NKG2C activating receptor could play a crucial role in NK cell expansion and/or maturation driven by HCMV infection.. In the present study, we analyzed the maturation and function of NK cells developing in three patients transplanted with cord blood cells from donors carrying homozygous deletion of the NKG2C gene and undergoing HCMV infection. These cases offered a unique opportunity to analyze the features of NK cell ...
CMV IG is a sterilized solution made from human plasma. It contains the antibodies to help your body protect itself against infection with cytomegalovirus. CMV IG is used to help prevent infection by cytomegalovirus in people who receive an organ transplant (kidney, heart, liver, lung, or pancreas). CMV IG may also be...
1. MocarskiES. ShenkT. PassRF. 2007 Cytomegaloviruses. KnipeDM. HowleyPM. GriffinDE. LambRA. MartinMA. RoizmanB. StrausSE. Fields virology, 5th ed. Lippincott Williams & Wilkins, Philadelphia, PA 2701 2772. 2. MeierJL. StinskiMF. 2006 Major immediate-early enhancer and its gene products. In Cytomegaloviruses: Molecular Biology and Immunology ed. ReddehaseM. Norfolk Caister Academic Press 151 166. 3. BegoM. MaciejewskiJ. KhaiboullinaS. PariG. St JeorS. 2005 Characterization of an antisense transcript spanning the UL81-82 locus of human cytomegalovirus. J Virol 79 11022 11034. 4. KondoK. XuJ. MocarskiES. 1996 Human cytomegalovirus latent gene expression in granulocyte-macrophage progenitors in culture and in seropositive individuals. Proc Natl Acad Sci U S A 93 11137 11142. 5. PetrucelliA. RakM. GraingerL. GoodrumF. 2009 Characterization of a novel Golgi apparatus-localized latency determinant encoded by human cytomegalovirus. J Virol 83 5615 5629. 6. JenkinsC. AbendrothA. SlobedmanB. 2004 A novel ...
ABSTRACTIt has been reported that human cytomegalovirus (HCMV) miR-US25-2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering
Abstract: Human cytomegalovirus (HCMV) remains the most important cause of serious viral infections in pediatric transplant recipients. In these patients, early diagnosis of active HCMV infection is important since the development of HCMV disease may be prevented. Ganciclovir has been established as an effective treatment agent for active infection by HCMV. HCMV disease can occur from infection acquired by the transplanted organ or from re-activation of latent infection. The risk is highest within 2 months of transplantation. Several risk factors for disease have been identified and include: HCMV-positive donor, HCMV-negative recipient, lack of anti-viral prophylaxis, and receipt of cadaveric kidney or type of bone marrow transplant. Intense immunosuppression has also been implicated. For discriminate patients with active infection from those without such infection, tests that include the pp65 antigenemia assay (AGM) and DNA detection methods are describle. The polymarase chain reaction (PCR) is ...
cytomegalovirus definition: any of a group of herpesviruses that cause enlargement of the epithelial cells, esp. of the salivary glands: associated with pneumonia and with abnormalities in newborn infantsOrigin of cytomegalovirus cyto- + megalo- + virus...
1. ReddehaseMJ. 2002 Antigens and immunoevasins: opponents in cytomegalovirus immune surveillance. Nat Rev Immunol 2 831 844. 2. LanierLL. 2008 Evolutionary struggles between NK cells and viruses. Nat Rev Immunol 8 259 268. 3. LodoenMB. LanierLL. 2006 Natural killer cells as an initial defense against pathogens. Curr Opin Immunol 18 391 398. 4. BironCA. ByronKS. SullivanJL. 1989 Severe herpesvirus infections in an adolescent without natural killer cells. N Engl J Med 320 1731 1735. 5. JonjicS. BabicM. PolicB. KrmpoticA. 2008 Immune evasion of natural killer cells by viruses. Curr Opin Immunol 20 30 38. 6. PowersC. DeFilippisV. MalouliD. FruhK. 2008 Cytomegalovirus immune evasion. Curr Top Microbiol Immunol 325 333 359. 7. WilkinsonGW. TomasecP. StantonRJ. ArmstrongM. ProdhommeV. 2008 Modulation of natural killer cells by human cytomegalovirus. J Clin Virol 41 206 212. 8. LodoenMB. LanierLL. 2005 Viral modulation of NK cell immunity. Nat Rev Microbiol 3 59 69. 9. LjunggrenHG. KarreK. 1990 In ...
Human aging is characterized by expanded and altered adaptive immune responses to human CMV (HCMV). It is unclear whether this expansion has its origins in age-related homeostatic disturbances or viral reactivation, whether anti-CMV immune surveillance may still be effective, and what are the consequences of this expanded immune response for health and longevity. We conducted an observational cross-sectional study in groups of HCMV-seropositive subjects aged ≥65 y of variable health status to compare the intensity of Ab responses against HCMV with those against EBV and with CD4+ and CD8+ T cell proinflammatory effector responses directed to HCMV-derived pp65 and immediate-early protein 1 synthetic peptides. Ab responses to HCMV, but not to EBV, and anti-HCMV CD4+, but not CD8+, T cell responses were more intense in elderly subjects aged ≥85 y in poor health and were inversely correlated with markers of functional activity and cognitive function. Therefore, humoral and CD4+ T cell anti-HCMV ...
T-bet:Eomes balance, effector function, and proliferation of cytomegalovirus-specific CD8+ T cells during primary infection differentiates the capacity for durable immune control.