Studies were performed to investigate whether electrically-induced long-term depression (LTD) within rat hippocampal slices in vitro shares any common cellular features with LTD in the intact animal, with particular emphasis being placed on mechanisms required for its late maintenance. Our initial studies have led to the development of stimulation protocols which are able to reliably produce different forms of LTD. Depending on the induction protocol applied, we are able to demonstrate a transient protein synthesis-independent early-LTD with a duration of up to 3-4 h, together with a de novo protein synthesis-dependent late-LTD lasting for at least 8 h. Furthermore, we are able to show input-specific LTD within the CA1 region, with expression shown only by those synapses specifically stimulated by a low-frequency protocol. These studies are important pre-requisites to investigate mechanisms of synaptic tagging and late-associativity during LTD ...
Expression of the HTLV receptor was strictly dependent on de novo protein synthesis. It has been shown that prior to T-lymphocyte proliferation, there is a 7- to 10-fold increase in protein synthesis and a 30- to 40-fold augmentation in mRNA synthesis.33This "pre-proliferation" characteristic appears to be a particularity of T lymphocytes and is not observed in other cell types. The required pre-proliferation burst in mRNA/protein synthesis likely results from the extremely low metabolic rate of resting G0T lymphocytes, which make up the vast majority of the circulating T-lymphocyte pool. Thus, it is intriguing to speculate that it is this low metabolic rate that accounts for the fact that T lymphocytes are the first and only cell type, identified to date, which do not constitutively express the HTLV receptor. Notably, Wodarz and Bangham have recently used a mathematical model to suggest that the rate of evolution of HTLV-1 is limited by the restricted availability of activated uninfected T ...
Medrano, E E. and Pardee, A B., "Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest." (1980). Subject Strain Bibliography 1980. 3388 ...
Export Data And Price Of Cycloheximide , www.eximpulse.com Eximpulse Services is the place where you can find the recent and updated Trade intelligence report of Cycloheximide Export Data. Whole information is based on updated Export shipment data of Indian Customs. All the compilation is done on the basis of All India ports data and has been done on daily basis. This helps you to get all India Cycloheximide Export data. You can find previous two days Cycloheximide Export data on Eximpulse Services. Cycloheximide Export data can be useful in different kind of analysis such as: Export price, Quantity, market scenarios, Price trends, Duty optimization and many more. Some Sample Shipment records for Cycloheximide Export Data of India are mentioned above. Further for Free sample and pricing of detailed reports contact on [email protected] Data post 2012 as per Notification No.18/2012 - Customs(N.T.) and does not have names of Indian companies and Foreign Companies.. ...
We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.. ...
Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. Use the Botstein labs balance as it is much more accurate than ours. It seems to make the most sense to aim for about 100mg (but if it is slightly over or under that is fine) and then put this in a 2ml eppendorf or similar tube (you can use a 15ml conical if you are going to make a larger volume) and add the appropriate amount of DMSO to this based on what you weighed out. Cycloheximide is bad for you (see below) so be careful with it! ...
Fuhr, J E.; Overton, M; and Leisy, M, "Protective effect of cycloheximide upon protein synthesis by l5178y cells exposed to hyperthermia." (1974). Subject Strain Bibliography 1974. 2356 ...
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Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required.
Reference: Polikarpova L.I., Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of sexually mature male rats, Voprosy meditsinskoi khimii, 1974, vol: 20(2), 215-217 ...
Fertilization of metaphase II-arrested mouse eggs results in resumption of meiosis and a decrease in both cdc2/cyclin B kinase and MAP kinase activities; the decrease in cdc2/cyclin B kinase activity precedes the decrease in MAP kinase activity. Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase and MAP kinase activities that occur following fertilization, although cortical granule exocytosis is not stimulated. The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity ...
Fig. 5. Identification of Pax3 binding sites on Pax3 promoters in vitro (A) Pax3, but not Zic1 alone, can trigger synthesis of the endogenous Pax3 transcript (primers in 3UTR, not amplifying the inducible form) either in absence of the translation inhibitor cycloheximide (induction is 5-fold compared to uninjected animal caps) or in the presence of cycloheximide (induction is 31-fold, compared to cycloheximide-treated uninjected animal caps). Endogenous pax3 expression level in a stage 11 whole embryo is set at a relative value of 1 unit. After co-injection of Pax3 and Zic1, endogenous pax3 was activated as an immediate-early target as well (77-fold compared to uninjected animal caps; and, in presence of cycloheximide, 16-fold when compared to cycloheximide-treated uninjected animal caps). (B) Location of the ECR containing Pax3 putative binding site in the genomic sequence upstream of the Pax3 TSS. (C) Pax3 binds specifically to the motif identified in the pax3 promoter ECR: an electrophoretic ...
In DG cultures exposed to 10 mM NaCN for 45 min, ≈90% of neurons (identified by immunoreactivity against NeuN, a neuronal marker) died 24 h after hypoxia, because they stained with propidium iodide. Survival of GFAP-immunoreactive glia was not affected (data not shown). Combining the same cyanide treatment with glucose deprivation ("ischemia") had very similar effects on the neurons. By EM, many hypoxic neurons showed early swelling of mitochondria and endoplasmic reticulum, in the presence of an intact nucleus (Fig. 1B), and evolved later toward necrosis with severe cytoplasmic swelling and membrane rupture (Fig. 1C). This finding was in sharp contrast to staurosporine-treated apoptotic neurons, which displayed nuclear breakdown into large, round masses of chromatin (Fig. 1D). Hypoxic necrosis evolved rapidly: we observed a progressive loss of the mitochondrial membrane potential Δψ within a few minutes of cyanide application (Fig. 2 A-D). This Δψ decay was always synchronous with release ...
Treatment with the protein synthesis inhibitor cycloheximide (CHX) or silencing of the core component of the NMD machinery, Upf-1 (also known as Rent1), are widely used to determine if transcripts are subject to NMD (Montfort et al., 2006). CHX is an indirect NMD inhibitor since NMD activation is posttranscriptional, but translation-dependent, and ribosomal association is required during the pioneer round of translation. Upf-1 silencing is believed to produce direct suppression of NMD. The RNA helicase, Upf-1, is a central effector of NMD that links the translation-termination event to the assembly of a surveillance complex. Upf2 and Upf3 are believed to recruit and activate Upf1 on NMD substrates (Lykke-Andersen et al., 2000). Although the discrimination of PTCs from normal termination codons and the molecular links that trigger NMD are still unclear, it is well established that the core components of the NMD machinery are the conserved proteins, Upf-1, Upf-2 and Upf-3. PTC-containing mRNAs ...
Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. Cycloheximide acts by inhibiting elongation during protein synthesis.
A) Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. NCCIT cells were stimulated for 4 hours with CCM (C) or Wnt-3
TNFα, the founding member of the TNF-superfamily, mediates both necroptotic and apoptotic cell death (Walczak, 2011; Micheau and Tschopp, 2003; Andrew, 2004; Wang et al., 2008; Kroemer et al., 2007; Degterev and Yuan, 2008, He et al., 2009; Zhang et al., 2009). Although many investigations have been conducted over the last few years the detailed mechanisms of these death signaling pathways are still not completely known. This particularly concerns our understanding about where ROS are produced intracellularly, how ROS and LMP are mechanistically linked and how both processes contribute to TNFα-induced apoptosis (Boya and Kroemer, 2008; Shen and Pervaiz, 2006; Zdolsek et al., 1993; Brunk et al., 1995). Our study here bridges these missing gaps. We identified that TNFα/CHX-induced LMP is not an apoptosis initiating, but amplifying process downstream of MOMP, and it is triggered by mitochondrial ROS generated as the result of a caspase-3-mediated cleavage of the p75 NDUFS1 subunit of complex I. ...
106. Abstract Protoplasts of Boergesenia forbesii (Hanvey) were treated with inhibitors of protein synthesis in order to investigate their effects on cellulose synthesis. Cellulose synthesis was reversibly inhibited by 10 M cycloheximide as assayed by fluorescence microscopy of Tinopal binding to cellulose. Freeze fracture and image analysis of cycloheximide-treated cells indicated a reduction in the number of intramembrane particles; however, the terminal synthesizing complexes remained at all times. Treatment with 10 M actinomycin D, when applied during the first hour of protoplast formation, irreversibly inhibits cellulose synthesis and terminal complex formation. De novo protein synthesis is required for cell wall regeneration by protoplasts. The data suggest that the structural subunits visualized in the terminal complex do not undergo signifi-cant turnover, but that there may exist an essential proteinaceous component of cellulose synthesis which must be continually renewed ...
Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.[1] Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm[2]), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreasing as the health risks have become ...
Memory can be divided according to different temporal phases: acquisition, consolidation, and retrieval. The consolidation phase is divided into molecular consolidation (range of hours after acquisition) and system consolidation (range of weeks and months after acquisition) (Dudai, 2004). Molecular consolidation is thought to be a protein synthesis-dependent process (Alberini, 2008; Costa-Mattioli et al., 2009). The clear effect of protein synthesis inhibitors on long-term memory was studied extensively mainly in the context of late-phase long-term potentiation (LTP) (Abraham and Williams, 2008). Similar molecular mechanisms were proposed for maintenance of long-term synaptic modifications and learning processes (Davis and Squire, 1984; Costa-Mattioli et al., 2009). However, long-term memory is not supported only by modulation of synaptic strength; modifications in intrinsic neuronal properties also subserve learning-related behavioral changes (Saar and Barkai, 2003; Zhang and Linden, 2003; ...
Summary Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. Consistent with these observations was the finding of a build-up of polyribosomes but an absence of nucleocapsids in the infected cells. These results suggest that measles virus RNA replication, but not transcription, is dependent upon active protein synthesis.
For immunofluorescence, 13-mm-diameter glass coverslips were derivatized for 30 min with 1 mM sulpho-m-maleimidobenzoyl-N-hydrosuccinimide ester (Perbio Science). For biochemical assays, 15-cm tissue culture-treated plastic dishes were coated directly with ligand. Coverslips or dishes were coated for 2 h at room temperature with 10 μg/ml fibronectin polypeptides in Dulbeccos PBS containing calcium and magnesium (Biowhittaker UK) and blocked with 10 mg/ml of heat-denatured BSA for 30 min at room temperature. Equivalent ligand coating between glass and plastic was tested by ELISA using the antifibronectin mAb 333 (Bass et al., 2007). For experiments on defined ligands, cells were treated with 25 μg/ml cycloheximide (Sigma-Aldrich) for 2 h before detachment to prevent de novo matrix synthesis and were then detached with 0.5 mg/ml trypsin. Cells were resuspended in DME/25 mM Hepes and 25 μg/ml cycloheximide, plated at a density of 1.25 × 104 cells per coverslip or 4 × 106 cells per dish, and ...
SR9243 is a potent and selective LXR inverse agonist. SR9243 kills cancer cells by inhibiting lipid production and the Warburg effect. SR9243 induces cell death in multiple types of cancer and does not cause the side effects that have derailed previous attempts to target these processes. In cancer cells, SR9243 significantly inhibited the Warburg effect and lipogenesis by reducing glycolytic and lipogenic gene expression. SR9243 induced apoptosis in tumors without inducing weight loss, hepatotoxicity, or inflammation. Malignant cells exhibit aerobic glycolysis (the Warburg effect) and become dependent on de novo lipogenesis, which sustains rapid proliferation and resistance to cellular stress.
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Buy Anisomycin (CAS 22862-76-6), a protein synthesis inhibitor. Join researchers using high quality Anisomycin from Abcam and achieve your mission, faster.
Cerebellar granule cells are susceptible to the excitotoxin glutamate, which acts at N-methyl-D-aspartate (NMDA) receptors, as well as the neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+), the active cytotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Paradoxically, preincubation of cultured cerebellar granule cells with low concentrations of NMDA or glutamate markedly antagonizes the neurotoxicity resulting from subsequent exposure to toxic concentrations of either MPP+ or glutamate. The neuroprotective effects of NMDA and glutamate against MPP+ toxicity are observed at agonist concentrations as low as 1 microM, are blocked by specific NMDA receptor antagonists, and require at least 30 min to develop fully. Moreover, NMDA receptor-mediated neuroprotection is prevented by the RNA synthesis inhibitor actinomycin D or the protein synthesis inhibitor cycloheximide. Thus, in cerebellar granule cells activation of NMDA receptors by glutamate can result in either ...
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Rasch et al. recently published an article describing antimicrobial properties of cycloheximide derivatives with adamantyl moieties against Legionella pneumophila. Ten different cycloheximide derivatives were synthesized in this study, and five were found to inhibit L. pneumophila growth at a concentration of 30 uM or 40 uM. Interestingly, several other clinically significant Gram-positive and Gram-negative pathogens were not susceptible to these derivatives which suggests a very narrow spectrum of activity which may be limited specifically to L. pneumophila ...
SABONNER; Plus de 88 millions dutilisateurs sur POF se réunissent pour se connecter, flirter, et partager avec chacun, ce qui donne plus de 10 millions de.The page you are looking for is no longer available: Comparative Study of Propranolol hydrochloride Release from Matrix Tablets with Kollidon®SR or Hydroxy Propyl.Propranolol has been used for MYOCARDIAL INFARCTION; ARRHYTHMIA; ANGINA PECTORIS; HYPERTENSION;. ATC code(s) propranolol and other combinations [ATC Code].mechanism(s). Materials and. oxide and cyclic guanosine monophosphate synthesis inhibitors on the extract effects were evaluated. Propranolol and N.Il sagit de choix des ouvrages, choix des genres, choix des textes, choix dauteurs en fonction du contexte socioculturel et politique de lapprenant.Propranolol Tablets Ip 40 Mg, Propranolol Generic And Trade Name. over the counter propranolol, propranolol urine drug test.. Titre du document / Document title Synthesis and chromatographic separation of the glucuronides of (R)- and ...
Bioprocess intensification can be achieved through high cell density perfusion cell culture with continuous protein capture integration. Protein passage and cell retention are commonly accomplished using tangential flow filtration systems consisting of microporous membranes. Significant challenges, including low efficiency and decaying product sieving over time, are commonly observed in these cell retention devices. Here, we demonstrate that a macroporous membrane overcomes the product sieving challenges when comparing to several other membrane chemistries and pore sizes within the microporous range. Learn More. ...
The mechanism of protein synthesis on 70S ribosomes includes the following stages: 1. The transcription: The process of protein synthesis is started by the uncoiling of strands of DNA molecule. One strand of DNA molecule acts as a template for the formation of mRNA. The mRNA is formed according to the triplet codes of DNA […]. ...
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
Figure 5. A, HEC1A cells transfected with LacZ or p85α WT or E160* were treated with cycloheximide (CHX; left) for indicated duration or with MG132 (center) for 24 hours. Cells were then harvested for WB. PTEN levels were normalized to β-actin by densitometry (below). *, P , 0.05. Right, HEC1A cells were transfected with LacZ or WT or E160* in the absence or presence of ubiquitin (Ub) for 72 hours. Whole-cell lysates were collected for IP with anti-PTEN and then subjected to WB with anti-ubiquitin. PTEN protein levels were normalized prior to IP by using proportionally different amounts of lysates. B, left, HEC1A cells were transfected with LacZ or WT or its mutants for 72 hours and were collected for IP with PTEN and WB with anti-p85α. HEC1A cells were co-transfected with WT or increasing amount of E160*. Cell lysates were collected for IP (center) or WB (right top). Right bottom, transfected HEC1A cells were treated with CHX for the indicated time points and harvested for WB. C, cells were ...
This antibiotic originating from the Gram+ actinomycete Streptomyces venezulenza has about the same type of activity as cycloheximide, but than with an ...
Two lipid synthesis inhibitors compounds (spiromesifen SPM and spirotetramat SPT) were used against the second instar larvae of cotton leaf worm, Spodoptera littoralis under laboratory condition. Toxicity of both compounds was tested. Effect of these insecticides on total lipid also was determined. The obtained results showed that both compounds were moderately toxic against the second instar larvae of S. littoralis. The mortality percentages caused by the field rate of spiromesifen and spirotetramat were 51.7 and 60.0%, respectively. The lethal concentrations for 50% of tested insect population (LC50) were 170.1 and 42.2 ppm, respectively. The total lipid content was determined in treated larvae and compared with the untreated. The percentage of total lipid in spiromesifen- and spirotetramat-treated larvae was 2.17 and 2.21%, respectively, compared with 2.42% in untreated larvae. GC/MS also was used in identification of fatty acids composition of S. littoralis larvae. The results cleared that 13 fatty
Mild hyperthermia is known to enhance apoptosis in a range of normal and neoplastic cell populations. Studies of tumours previously shown to respond to heating in this manner might be expected to provide insights not only into the mechanism of hyperthermic cell killing, but also into the apoptotic process in general. In the present study, cell death induced by 43°C heating for 30 min in two human Burkitts lymphoma lines, BM 13674 and WW1, and in murine mastocytoma P‐815 × 2·1 was found to be exclusively apoptotic in type, identification being based on light and electron microscopic appearances and on the presence of internucleosomal cleavage of DNA into fragments that are multiples of 180-200 base pairs, which was demonstrated by agarose gel electrophoresis. The heat‐induced apoptosis was prevented by the presence of zinc sulphate, an inhibitor of the endonuclease considered to be responsible for the DNA cleavage, but was not suppressed by the protein synthesis inhibitor cycloheximide. ...
An in vitro ischemia model was established and the effect of the metabolic inhibitors cycloheximide (CHX) and actinomycin D (ActD) on apoptosis in astrocytes under ischemia studied. CHX decreased by 75% the number of cells dying after 6 hr of ischemia compared with control cultures. TdT-mediated dUTP nick end labelling (TUNEL) staining of comparable cultures was reduced by 40%. ActD decreased cell death by 60% compared with controls. The number of TUNEL-positive cells was reduced by 38%. The nuclear shrinkage in TUNEL-positive astrocytes in control cultures did not occur in ActD-treated astrocytes, indicating that nuclear shrinkage and DNA fragmentation during apoptosis are two unrelated processes. Expression of bcl-2 (alpha and beta), bax, and Ice in astrocytes under similar ischemic conditions, as measured by quantitative reverse transcription-polymerase chain reaction, indicated that ischemia down-regulated bcl-2 (alpha and beta) and bax. Ice was initially down-regulated from 0 to 4 hr, ...
We used the rat visual cortex as a model system to examine the changes in protein synthesis during experience-induced synaptic plasticity. Dark-rearing rats from birth results in a relatively immature visual cortex that maintains the high de- gree of synaptic plasticity characteristic of the critical period (Kirkwood et al., 1995). Exposure of dark-reared rats to light results in a rapid, robust and coordinated burst of experience- driven synaptic plasticity that can be readily monitored at the biochemical and electrophysiological level (Quinlan et al., 1999). In previous work, we showed that visual experience evokes the polyadenylation of ␣-CaMKII mRNA in visual cortex and the elevation of ␣-CaMKII protein in synaptic fractions from this brain region. Moreover, this increase was a direct result of new synthesis because it was sensitive to the translation inhibitor cycloheximide (Wu et al., 1998). Here we show that the experience-induced increase of ␣-CaMKII pro- tein does not require new ...
Thymidine, a pyrimidine deoxynucleoside, is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. - Mechanism of Action & Protocol.
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What are the steps involved in protein synthesis when you start with the DNA code in the nucleus and end up with a polypeptide in simple terms please? Find answers now! No. 1 Questions & Answers Place.
The term protein synthesis refers to the making of a protein. This process begins in the nucleus and then continues in the ribosomes. Protein synthesis is comprised of two parts: DNA transcription...
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BA is a novel antineoplastic agent with cytotoxicity against melanoma and neuroectodermal tumor cells. Here, we report that BA triggers an apoptotic cascade in human malignant glioma cells as well. BA-induced apoptosis of glioma cells involves sequentially new protein synthesis, formation of reactive oxygen species, and caspase processing. In detail, we show that BA toxicity depends on new protein but not mRNA synthesis, suggesting that translation of preexisting mRNA mediates BA toxicity. Caspase 8 and 3 activation are critical steps in the killing cascade triggered by BA because cell death ensues whenever caspase activation is detected. Furthermore, pharmacological (PBN, NAC, DEVD-CHO) or genetic (BCL-2) manipulations that inhibit apoptosis do so at the level of, or upstream of, caspase activation. Cytotoxic, cytokine-resistant glioma cells do not exhibit cross-resistance to BA, suggesting that caspase activation is independent of interactions of endogenous CD95/CD95L or related pairs of death ...
In the present study, we have shown that the PNC actively incorporates Br-UTP and FITC-CTP in a transcription assay using permeabilized cells. Many studies have used this assay to investigate transcription patterns in the cell nucleus (Hozak et al., 1994; Aoki et al., 1997; Fay et al., 1997; Neugebauer and Roth, 1997) since its initial establishment (Jackson et al., 1993; Wansink et al., 1993). Our observation that the PNC actively incorporates labeled nucleotides in a DNA-dependent manner after 5 min of pulse labeling suggests that the PNC is a site of transcription. Inhibition of RNA polymerase I either by addition of actinomycin D in the transcription cocktail or by pretreatment of cells with cycloheximide does not affect the Br-UTP incorporation, indicating that transcription of nascent RNA in the PNC is unlikely to involve RNA polymerase I. This finding is consistent with the report by Matera et al. (1995) that 28S rRNA was not detected in the PNC using in situ hybridization and our ...
Bleomycin sulfate is a mixture of the sulfate salts of basic glycopeptide antineoplastic antibiotics isolated from Streptomyces verticillus.
I am interested in finding protocols for inhibiting protein and RNA synthesis in vivo in Arabidopsis. Specifically, I am interested in learning the appropritate concentrations of inhibitors such as, but not limited to, cyclohexamide and actinomycin D to use on intact plants, and the time it takes after treatment before one can expect to detect the inhibition. Also, I am interested in protocols that allow one to quantitate the effectiveness of the inhibitors, ie. how to use S35 Met and tritiated uracil to determine if the concentration of the inhibitors and the timing of the treatments were appropriate. Any information will be appreciated. Thanks. Dave Horvath MFT at CLVAX1.CL.MSU.EDU ...
The fundamental, continuing goal of the proposed research is to investigate the potential importance of Ca2+ as a physiologic regulator of protein synthesis in...
Protein synthesis is important because the proteins created during this process control the activities of the cells. Without these proteins, many of the processes in the body would fail or not work...