BACKGROUND & AIMS: Fibrolamellar hepatocellular carcinoma (FL-HCC) is a primary liver cancer that predominantly affects children and young adults with no underlying liver disease. A somatic, 400 Kb deletion on chromosome 19 that fuses part of the DnaJ heat shock protein family (Hsp40) member B1 gene (DNAJB1) to the protein kinase cAMP-activated catalytic subunit alpha gene (PRKACA) has been repeatedly identified in patients with FL-HCC. However, the DNAJB1-PRKACA gene fusion has not been shown to induce liver tumorigenesis. We used the CRISPR/Cas9 technique to delete in mice the syntenic region on chromosome 8 to create a Dnajb1-Prkaca fusion and monitored the mice for liver tumor development.. METHODS: We delivered CRISPR/Cas9 vectors designed to juxtapose exon 1 of Dnajb1 with exon 2 of Prkaca to create the Dnajb1-Prkaca gene fusion associated with FL-HCC, or control Cas9 vector, via hydrodynamic tail vein injection to livers of 8-week-old female FVB/N mice. These mice did not have any other ...

PRKACA - PRKACA (untagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 available for purchase from OriGene - Your Gene Company.

PRKACA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 578 amino acids.

PRKACB - Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3 , Myc-DDK-tagged available for purchase from OriGene - Your Gene Company.

Wiki-Pi: a web resource for human protein-protein interactions. It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations including cellular localization, molecular function and biological process, drugs, diseases, genome-wide association studies (GWAS), GO enrichments, PDB ID, Uniprot ID, HPRD ID, and word cloud from pubmed abstracts.

Wiki-Pi: a web resource for human protein-protein interactions. It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations including cellular localization, molecular function and biological process, drugs, diseases, genome-wide association studies (GWAS), GO enrichments, PDB ID, Uniprot ID, HPRD ID, and word cloud from pubmed abstracts.

DLX3 is a novel target of PRKACA, and PRKACA mediates BMP signaling during osteoblast differentiation, at least in part, by phosphorylating DLX3 and modulating the protein stability and function of DLX3 ...

Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.

Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3, mRNA. (H00005567-R05) - Products - Abnova

KAPCA_HUMAN] Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, TRPC1 and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and ...

O95819 (MAP4K4) , Q5VT25 (CDC42BPA) , P41279 (MAP3K8) , Q04759 (PRKCQ) , O75116 (ROCK2) , Q13464 (ROCK1) , Q5S007 (LRRK2) , P25098 (GRK2) , P17612 (PRKACA) , Q9Y5S2 (CDC42BPB) , O94804 (STK10 ...

DGIdb, The Drug Gene Interaction Database, is a research resource that can be used to search candidate genes or drugs against the known and potentially druggable genome.

Mouse polyclonal antibody raised against a partial recombinant RAPGEF3. RAPGEF3 (AAH17728, 772 a.a. ~ 881 a.a) partial recombinant protein with GST tag. (H00010411-A01) - Products - Abnova

Fibrolamellar hepatocellular carcinoma (FL-HCC) is a rare liver tumor affecting adolescents and young adults with no history of primary liver disease or cirrhosis. We identified a chimeric transcript that is expressed in FL-HCC but not in adjacent normal liver and that arises as the result of a ∼400-kilobase deletion on chromosome 19. The chimeric RNA is predicted to code for a protein containing the amino-terminal domain of DNAJB1, a homolog of the molecular chaperone DNAJ, fused in frame with PRKACA, the catalytic domain of protein kinase A. Immunoprecipitation and Western blot analyses confirmed that the chimeric protein is expressed in tumor tissue, and a cell culture assay indicated that it retains kinase activity. Evidence supporting the presence of the DNAJB1-PRKACA chimeric transcript in 100% of the FL-HCCs examined (15/15) suggests that this genetic alteration contributes to tumor pathogenesis ...

The Khoikhoi people who lived in the Cape Peninsula when the Dutch began trading with the area during the mid 17th century, had a hunting dog which was described as ugly, but noted for its ferocity when acting as a guard dog. This dog measured approximately 18 inches (46 cm) at the withers, with a lean but muscular frame. The ears have been described both as erect and hanging, but the most distinctive feature was the length of hair often growing in the reverse direction along its back. Within 53 years of Dutch colonisation in Southern Africa and the origins of the wagon-trekking boers later known as Afrikaners, who converted vast stretches of wild veld into farmland, hunted for meat and defended their cattle herds, staff, and homesteads from lion, the Europeans were using these local dogs themselves.[3]. By the 1860s, European colonisers had also imported a variety of mainly European dog breeds to this area of Africa, including such dedicated hunting dogs as Great Danes, Bloodhounds, Greyhounds, ...

Somatic mutations in protein kinase A catalytic α subunit (PRKACA) were found to be causative for 30-40% of cortisol-producing adenomas (CPA) of the adrenal gland, rendering PKA signalling constitutively active. In its resting state, PKA is a stable and inactive heterotetramer, consisting of two catalytic and two regulatory subunits with the latter inhibiting PKA activity. The human genome encodes three different PKA catalytic subunits and four different regulatory subunits that are preferentially expressed in different organs. In normal adrenal glands all regulatory subunits are expressed, while CPA exhibit reduced protein levels of the regulatory subunit IIβ. In this study, we linked for the first time the loss of RIIβ protein levels to the PRKACA mutation status and found the down-regulation of RIIβ to arise post-transcriptionally. We further found the PKA subunit expression pattern of different tumours is also present in the zones of the normal adrenal cortex and demonstrate that the different

Capacitation encompasses the molecular changes sperm undergo to fertilize an oocyte, some of which are postulated to occur via a cAMP-PRKACA (protein kinase A)-mediated pathway. Due to the recent discovery of cAMP-activated guanine nucleotide exchange factors RAPGEF3 and RAPGEF4, we sought to investigate the separate roles of PRKACA and RAPGEF3/RAPGEF4 in modulating capacitation and acrosomal exocytosis. Indirect immunofluorescence localized RAPGEF3 to the acrosome and subacrosomal ring and RAPGEF4 to the midpiece in equine sperm. Addition of the RAPGEF3/RAPGEF4-specific cAMP analogue 8-(p-chlorophenylthio)-2-O-methyladenosine-3,5-cyclic monophosphate (8pCPT) to sperm incubated under both noncapacitating and capacitating conditions had no effect on protein tyrosine phosphorylation, thus supporting a PRKACA-mediated event. Conversely, activation of RAPGEF3/RAPGEF4 with 8pCPT induced acrosomal exocytosis in capacitated equine sperm at rates (34%) similar (P > 0.05) to those obtained in ...

RgdA of A. fumigatus is a homolog of the Mbp1 protein of the budding yeast S. cerevisiae, a putative APSES TF. APSES TFs function as key regulators of fungal morphogenesis and development present in Aspergillus genomes, but only the stuA gene has been studied in A. fumigatus (10, 12, 14, 15, 17). The deletion of rgsA resulted in decreased mycelial growth and conidiation and reduced mRNA levels of key asexual development regulators compared to WT (Fig. 2). The mRNA levels of PKA signaling components were higher in the ΔrgdA strain than in WΤ and complemented strains. Furthermore, the ΔrgdA strain showed higher PKA activity in the absence of cAMP (Fig. 3), suggesting the presence of free PKA catalytic subunits in the cytoplasm (18). Based on these observations, we can propose that RgdA of A. fumigatus is necessary for normal growth and proper asexual development, which may involve regulation of the cAMP-PKA signaling pathway.. The Dpr proteins are known to be activated by the stress-activated ...

Expression of RAPGEF3 (bcm910, cAMP-GEFI, EPAC) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.

Expression of RAPGEF3 (bcm910, cAMP-GEFI, EPAC) in breast tissue. Antibody staining with HPA040365, HPA043518 and CAB004386 in immunohistochemistry.

Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

Complete information for RAPGEF3 gene (Protein Coding), Rap Guanine Nucleotide Exchange Factor 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Fibrolamellar hepatocellular carcinoma (FHCC) is a rare form of hepatocellular carcinoma (HCC) that typically affects young adults and is characterized, under the microscope, by laminated fibrous layers interspersed between the tumour cells. Approximately 200 new cases are diagnosed worldwide each year. A recent study showed the presence of the DNAJB1-PRKACA chimeric transcript (resulting from a 400kb somatic deletion on chromosome 19) in 100% of the FHCCs examined (15/15) This gene fusion has been confirmed in a second study. The histopathology of FHCC is characterized by laminated fibrous layers, interspersed between the tumor cells. Cytologically, the tumor cells have a low nuclear to cytoplasmic ratio with abundant eosinophilic cytoplasm. Tumors are non-encapsulated, but well circumscribed, when compared to conventional HCC (which typically has an invasive border). Due to lack of symptoms, until the tumor is sizable, this form of cancer is often advanced when diagnosed. Symptoms include ...

Upregulation of A-kinase-interacting protein 1 (AKIP1) has been observed in breast and esophageal cancers, indicating that AKIP1 may be a potent oncogenic protein. However, the role of AKIP1 in cervical cancer still remains unknown. This study aimed to explore the role of AKIP1 in cervical cancer an …

PHACE association is a rare neurocutaneous condition in which facial hemangiomas associate with a spectrum of posterior fossa malformations, arterial cerebrovascular anomalies, cardiovascular anomalies, and eye anomalies. We reported a case of PHACE

cansSAR 3D Structure of 5N3F | CAMP-DEPENDENT PROTEIN KINASE A FROM CRICETULUS GRISEUS IN COMPLEX WITH FRAGMENT LIKE MOLECULE N-[3-(AMINOMETHYL)PHENYL]ACETAMIDE | 5N3F_A | cAMP-dependent protein kinase catalytic subunit alpha - Also known as KAPCA_CRIGR, PRKACA. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B

Lockheed Martin unveiled the HULC system at the Association of the United States Army Winter Symposium held in Fort Lauderdale, Florida, in February 2009.. In July 2010, the company signed a $1.1m contract with the US Army Natick Soldier Center for testing and evaluating the ruggedised HULC design.. Under the contract, the Natick Soldier Center tested the HULC for its effect on the soldiers performance, the energy that a soldier spends while using it and the adaptability of the system while carrying various loads and moving at various speeds. The contract has also provided provision for field trials of the system.. The HULC underwent laboratory testing in October 2010, after Lockheed Martin upgraded the ruggedised system for flexibility and suitability to a variety of users. The system was put through biomechanical, dynamic load and environmental testing.. Treadmill testing measured the decrease in metabolic cost of the user. The systems sustainability in various environmental conditions was ...

Next-day shipping cDNA ORF clones derived from RAPGEF5 Rap guanine nucleotide exchange factor 5 available at GenScript, starting from $99.00.

The adoptive parents may be acquainted with to these problems, but in other situations wee if any story may be available. N L H Genes 50 10 10 KREMEN2, ST8SIA1, TNFSF10, ATF6 150 30 30 HADHB, BAX, MAPK13, CYP1A1, ATF2 250 49 51 NFKBIA, PLCB1, ITGB1, MYC, KRAS 350 69 71 PRKACB, FOS, PRKACG, FASN, NFKB1 450 85 95 RAC1, MAP2K2, JUN, TP53, RELA 551 100 119 RAF1, GRB2, PIK3CA, RPS27A, MAPK8 647 117 145 HRAS, MAP2K1, AKT1, RAF1, GRB2 765 125 185 MAPK3, MAPK1, HRAS, MAP2K1, AKT1 Provisions 7. Pregnancy and Yeast InfectionThe Main Benefits: 1 cheap kamagra effervescent 100mg fast delivery erectile dysfunction injections videos. At hand drowning is described as an fact in which a offspring has suffered a submersion injury and has survived for at least 24 hours. It involves removing the little one from the fine kettle of fish area and placing him or her in a dispassionate, nonthreatening, safe square footage where no interaction occurs between the daughter and parents or others exchange for a specifically ...

Highly up-regulated in liver cancer (HULC) was originally identified as the most overexpressed long non-coding RNA in hepatocellular carcinoma. Since its discovery, the aberrant up-regulation of HULC has been demonstrated in other cancer types, including gastric cancer, pancreatic cancer, osteosarcoma and hepatic metastasis of colorectal cancer. Recent discoveries have also shed new light on the upstream molecular mechanisms underlying HULC .... Read More » ...

According to Berkeley Bionics and Lockheed Martin, the HULC biomechanical combat exoskeleton system enables a soldier between 5′ 4″ and 6′ 2″ to carry loads of up to 200 pounds (200 lbs) on the front or back for extended periods of times over any and all terrains by transfering the load weight and soldiers weight to the ground. The load-carrying augmentation/enhancement ability aspect will continue to work when battery power isnt available. The HULC also allows a soldier to run 7 mph for long periods or 10 mph in short bursts. It provides the soldier with full range of motion and, according to the developers, doesnt hinder or impede the warfighters movement in any way/aspect, so he/she can still jump, squat, kneel, crawl, go prone (for survival and stabilized or long-range shooting), etc., and then get right back up.. One of Defense Reviews immediate and primary concerns is whether or not the HULC combat exoskeleton allows the same level of mobility and dexterity required for ...

Fremmenik yaks are obtained from killing yaks on Neitiznot at rate of 1/3,000. They are used in the Player-owned farm. It requires level 71 Farming to check. The drop shows as a baby yak, not as a drop. Upon obtaining a Fremennik yak, the player receives the following message: A baby yak starts...

Hard Drive died and I cant find a image file of the operating system. Any help is greatly appreciated. Also...I tried the mfslive.iso test...

reference: ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail., Bertran MT, Mouilleron S, Zhou Y, Bajaj R, Uliana F, Kumar GS, van Drogen A, Lee R, Banerjee JJ, Hauri S, OReilly N, Gstaiger M, Page R, Peti W, Tapon N, Nat Commun. 2019 Feb 15;10(1):771. doi: 10.1038/s41467-019-08686-0. PMID: 30770806 ...

မန္မာႏိုင္ငံမွာေတာ့ ေ၀လငါးဖမ္းလုပ္ငန္းကို လုပ္ကိုင္တဲ့လူမရွိေသးဘူးလို႕ ထင္ပါတယ္။ ျမန္မာ့ပင္လယ္ျပင္မွာ ေ၀လငါးဟာ အင္မတန္ေတြ႕ရခဲတာေၾကာင့္ရယ္၊ ေတြ႕ရင္ေတာင္မွ အရြယ္အစားၾကီးမားလြန္းတာရယ္နဲ႕ ေရာင္းခ်မယ့္ေစ်းကြက္မရွိတာရယ္ေၾကာင့္ ဖမ္းဆီးမယ့္လူမရွိပါဘူး။ ေနာက္တစ္ခ်က္ လင္းပိုင္နဲ႕ေ၀လငါးဟာ အသိဥာဏ္ရွိတဲ့ႏို႕တိုက္သတၱ၀ါျဖစ္လို႕ တခါတရံမွာ ...

Genetic variants in components of the protein kinase A (PKA) enzyme have been associated with various defects and neoplasms in the context of Carney complex (CNC) and in isolated cases, such as in primary pigmented nodular adrenocortical disease (PPNAD), cortisol-producing adrenal adenomas (CPAs), and various cancers. PRKAR1A mutations have been found in subjects with impaired cAMP-dependent signaling and skeletal defects; bone tumors also develop in both humans and mice with PKA abnormalities. We studied the PRKACB gene in 148 subjects with PPNAD and related disorders, who did not have other PKA-related defects and identified two subjects with possibly pathogenic PRKACB gene variants and unusual bone and endocrine phenotypes. The first presented with bone and other abnormalities and carried a de novo c.858_860GAA (p.K286del) variant. The second subject carried the c.899C,T (p.T300M or p.T347M in another isoform) variant and had a PPNAD-like phenotype. Both variants are highly conserved in the ...

Authors: Vázquez-Higuera, José Luis , Mateo, Ignacio , Sánchez-Juan, Pascual , Rodríguez-Rodríguez, Eloy , Pozueta, Ana , Calero, Miguel , Dobato, José Luis , Frank-García, Ana , Valdivieso, Fernando , Berciano, José , Bullido, Maria J. , Combarros, Onofre Article Type: Research Article Abstract: Tau abnormal hyperphosphorylation and the formation of neurofibrillary tangles in the Alzheimers disease (AD) brain is the result of upregulation of tau kinases. In a group of 729 Spanish late-onset AD patients and 670 healthy controls, we examined variations into a set of 20 candidate genes of kinases involved in tau phosphorylation at AD-related sites (PRKACB; CAMK2A; MARK1, 2, 3 and 4; CSNK1D; CDC2; RPS6KB1 and 2; p38α and β; IB1; JNK1, 2 and 3; MEK1 and 2; ERK1 and 2), to address hypotheses of genetic variation that might influence both AD risk and age at disease onset. There was …an increased frequency of RPS6KB2 (intron 2, rs917570) minor allele in patients (50%) versus controls (39%) ...

Touch panel maker TPK Holding suffered a net loss per share of NT$7.28 (US$0.228) for the second quarter of 2016, the company disclosed at a July 28 investors conference.

I drink straight whiskey, and actually enjoy it. I can understand the standpoint of a non-whiskey drinker, in the same way that I often wonder how anyone can