Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a newly described Ca2+-mobilizing nucleotide that appears to target intracellular Ca2+-release channels distinct from those sensitive to inositol trisphosphate or ryanodine/cyclic ADP-ribose. Little, however, is known concerning the regulation of cellular NAADP levels. In the present study, we have characterized the metabolism of NAADP by brain membranes. From HPLC and MS analyses we show that loss of NAADP was associated with the appearance of a major product that is likely to be nicotinic acid-adenine dinucleotide (NAAD), the dephosphorylated form of NAADP. Dephosphorylation of NAADP, but not 3-NAADP, was dramatically attenuated by Ca2+ chelators and stimulated by Ca2+ over a physiological range in a calmodulin-insensitive manner. In contrast, NADP was metabolized predominantly to ADP-ribose phosphate via glycohydrolase activity, although slower Ca2+-dependent dephosphorylation of both NADP and 2-AMP could also be demonstrated. This is the

Cyclic adenosine diphosphate ribose (cADPR) is a potent endogenous calcium-mobilizing agent synthesized from beta-NAD+ by ADP-ribosyl cyclases in sea urchin eggs and in several mammalian cells (Galione, A., and White, A. (1994) Trends Cell Biol. 4, 431 436). Pharmacological studies suggest that cADPR is an endogenous modulator of Ca2+-induced Ca2+ release mediated by ryanodine-sensitive Ca2+ release channels. An unresolved question is whether cADPR can act as a Ca2+-mobilizing intracellular messenger. We show that exogenous application of nitric oxide (NO) mobilizes Ca2+ from intracellular stores in intact sea urchin eggs and that it releases Ca2+ and elevates cADPR levels in egg homogenates. 8-Amino-cADPR, a selective competitive antagonist of cADPR-mediated Ca2+ release, and nicotinamide, an inhibitor of ADP-ribosyl cyclase, inhibit the Ca2+-mobilizing actions of NO, while, heparin, a competitive antagonist of the inositol 1,4,5-trisphosphate receptor, did not affect NO-induced Ca2+ release. Since the

In addition to its well established function in activating Ca(2+) release from the endoplasmic reticulum (ER) through ryanodine receptors (RyR), the second messenger cyclic ADP-ribose (cADPR) also accelerates the activity of SERCA pumps, which sequester Ca(2+) into the ER. Here, we demonstrate a potential physiological role for cADPR in modulating cellular Ca(2+) signals via changes in ER Ca(2+) store content, by imaging Ca(2+) liberation through inositol trisphosphate receptors (IP(3)R) in Xenopus oocytes, which lack RyR. Oocytes were injected with the non-metabolizable analog 3-deaza-cADPR, and cytosolic [Ca(2+)] was transiently elevated by applying voltage-clamp pulses to induce Ca(2+) influx through expressed plasmalemmal nicotinic channels. We observed a subsequent potentiation of global Ca(2+) signals evoked by strong photorelease of IP(3), and increased numbers of local Ca(2+) puffs evoked by weaker photorelease. These effects were not evident with cADPR alone or following cytosolic Ca(2+)

BACKGROUND AND PURPOSE: Recently, a number of mimics of the second messenger cyclic ADP-ribose (cADPR) with replacement of adenosine by inosine were introduced. In addition, various alterations in the molecule ranging from substitutions at C8 of the base up to full replacement of the ribose moieties still retained biological activity. However, nothing is known about the metabolic stability and cellular effects of these novel analogues. EXPERIMENTAL APPROACH: cADPR and the inosine-based analogues were incubated with CD38, ADP-ribosyl cyclase and NAD-glycohydrolase and metabolism was analysed by RP-HPLC. Furthermore, the effect of the analogues on cytokine expression and proliferation was investigated in primary T-lymphocytes and T-lymphoma cells. KEY RESULTS: Incubation of cADPR with CD38 resulted in degradation to adenosine diphosphoribose. ADP-ribosyl cyclase weakly catabolised cADPR whereas NAD-glycohydrolase showed no such activity. In contrast, N1-cyclic inosine 5-diphosphoribose (N1-cIDPR) was not

Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) mobilize Ca2+ from two different types of intracellular stores and through completely independent mechanisms. The two Ca2+ messengers are also structurally distinct. cADPR is a cyclic nucleotide derived from NAD, whi …

Clone REA465 recognizes the human CD157 antigen, a glycosylphosphatidylinositol (GPI) linked protein, which is also known as bone marrow stromal antigen 1 (BST-1) or cyclic ADP-ribose hydrolase 2. CD157, a member of the CD38 gene family, is an NAD-metabolizing ectoenzyme and a signaling molecule, which synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, former a second messenger that elicits calcium release from intracellular stores. It is mainly expressed by cells of the myeloid lineage, bone marrow stroma, and vascular endothelium. CD157 is also expressed by ovarian cancer epithelium and by peritoneal mesothelial cells, where it is implicated in tumor dissemination. Further, it is endowed with receptor-like features observed in different cell types and transduces signals by interacting with transmembrane partner molecules, a strategy shared by other GPI-anchored molecules. CD157 is involved in neutrophil polarization, adhesion, and motility and controls

Title: Role of CD38 in Airway Function. VOLUME: 2 ISSUE: 2. Author(s):Bit Na Kang, Alonso G.P. Guedes, K. G. Tirumurugaan, Joseph A. Jude, Deepak A. Deshpande, Reynold A. Panettieri, Yassine Amrani, Frances E. Lund, Timothy F. Walseth and Mathur S. Kannan. Affiliation:Department of Veterinary andBiomedical Sciences, College of Veterinary Medicine, University ofMinnesota, 1971 Commonwealth Avenue, Saint Paul, MN 55108, USA.. Keywords:Cyclic ADP-ribose, airway smooth muscle, calcium, cytokines, asthma. Abstract: CD38, a 45-kDa cell surface glycoprotein, is involved in the synthesis of the calcium mobilizing second messenger molecule cyclic ADP-ribose. Cyclic ADP-ribose is known to release calcium from the sarcoplasmic reticulum of airway smooth muscle cells. The pharmacological features of cyclic ADP-ribose-mediated calcium release in airway smooth muscle cells are distinct from those mediated by inositol 1,4,5-trisphosphate and involve activation of ryanodine receptor channels. In airway smooth ...

The human lymphocyte antigen CD38 has been shown to share sequence homology with ADP-ribosyl cyclase, the enzyme that catalyzes the conversion of NAD+ to cyclic ADP-ribose (cADPR), a potent Ca(2+)-mobilizing agent. In this study COS1 cells from African Green Monkey kidney were transiently transfected with CD38 cDNA, inducing expression of authentic CD38 on the cell surface. We demonstrate that CD38 expressed in this manner can convert NAD+ to cADPR in the extracellular medium as assessed by Ca2+ release from sea-urchin egg microsomes.

CD38 is a 42-kilodalton glycoprotein expressed extensively on B and T lymphocytes. CD38 exhibits a structural homology to Aplysia adenosine diphosphate (ADP)-ribosyl cyclase. This enzyme catalyzes the synthesis of cyclic ADP-ribose (cADPR), a metabolite of nicotinamide adenine dinucleotide (NAD +) with calcium-mobilizing activity. A complementary DNA encoding the extracellular domain of murine CD38 was constructed and expressed, and the resultant recombinant soluble CD38 was purified to homogeneity. Soluble CD38 catalyzed the formation and hydrolysis of cADPR when added to NAD+. Purified cADPR augmented the proliferative response of activated murine B cells, potentially implicating the enzymatic activity of CD38 in lymphocyte function ...

Although activation of M2 muscarinic receptors is classically known to inhibit adenylyl cyclase activity (Peralta et al., 1988) or to modify membrane potential by inhibiting Ca2+-activated K+ channel (Kotlikoff et al., 1992), it has been recently proposed that M2 muscarinic receptors may induce Ca2+ signals by activation of the cADPR pathway (White et al., 2003) or by stimulation of a voltage-dependent Ca2+ channel (Cav1.2b) via the phosphatidylinositol 3-kinase/PKC pathway (Callaghan et al., 2004). Activation of the cADPR pathway by ACh in duodenum myocytes is shown by: (1) inhibition of Ca2+ oscillations by application of the cADPR competitive antagonist (8Br-cADPR), (2) inhibition of ACh-induced Ca2+ oscillations by inhibitors of ADP-ribosyl cyclase (ZnCl2, anti-CD38 antibody) and (3) detection of ADP-ribosyl cyclase activity by fluorescence experiments as the enzyme cyclizes NGD+ (non fluorescent) to produce cGDPR, a fluorescent compound (Graeff et al., 1994). This method has been used ...

Ligation of the T-cell receptor/CD3 complex results in global Ca(2+) signals that are essential for T-cell activation. We have recently reported that these global Ca(2+) signals are preceded by localized pacemaker Ca(2+) signals. Here, we demonstrate for the first time for human T cells that an increase in signal frequency of subcellular pacemaker Ca(2+) signals at sites close to the plasma membrane, in the cytosol and in the nucleus depends on the type 3 ryanodine receptor (RyR) and its modulation by cyclic ADP-ribose. The spatial distribution of D-myo-inositol 1,4,5-trisphosphate receptors and RyRs indicates a concerted action of both of these receptors/Ca(2+) channels in the generation of initial pacemaker signals localized close to the plasma membrane. Inhibition or knockdown of RyRs resulted in significant decreases in (1) the frequency of initial pacemaker signals localized close to the plasma membrane, and (2) the frequency of localized pacemaker Ca(2+) signals in the inner cytosol. Moreover,

Cyclic ADP-ribose and hydrogen peroxide synergize with ADP-ribose in the activation of TRPM2 channels. Mol Cell. 2005 Apr 1;18(1):61-9. PMED ID: 15808509. ...

NAADP (nicotinic acid-adenine dinucleotide phosphate), the most potent Ca2+-mobilizing second messenger, is active in a wide range of organisms and cell types. Until now, all NAADP-producing enzymes have been thought to be members of the ADP-ribosyl cyclase family. ADP-ribosyl cyclases exhibit promiscuous substrate selectivity, synthesize a variety of products and are regulated in a limited manner, which may be non-physiological. In the present paper, we report the presence of an enzyme on the surface of sea urchin sperm that exhibits bell-shaped regulation by Ca2+ over a range (EC(50) of 10 nM and IC(50) of 50 microM) that is physiologically relevant. Uniquely, this surface enzyme possesses complete selectivity for nucleotides with a 2-phosphate group and exhibits only base-exchange activity without any detectable cyclase activity. Taken together, these findings indicate that this novel enzyme should be considered as the first true NAADP synthase.

Multiple mechanisms exist for increasing the concentration of intracellular calcium. This Perspective by Lee is one in a series on intracellular calcium release mechanisms and focuses on the calcium store operated by nicotinic acid adenine dinucleotide phosphate (NAADP). The characterization of the NAADP-operated calcium store as separate from the inositol trisphosphate (IP3)-operated and cyclic ADP-ribose (cADPR)-operated calcium stores is discussed. Lee also addresses the role of NAADP in regulating intracellular calcium fluctuations during fertilization and hormonal activation of pancreatic acinar cells.. ...

Nicotinamide adenine dinucleotide (NAD+) is the universal currency of energy metabolism and electron transfer. Recent studies indicate that apart from its role as a coenzyme, NAD+ and its metabolites also function in cell signaling pathways; for example, they are substrates for nucleotide-metabolizing enzymes and ligands for extra- and intracellular receptors and ion channels. Moreover, the NAD+ and NAD+ phosphate metabolites adenosine 5′-diphosphoribose (ADP-ribose), cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate (NAADP) have emerged as key second messengers in Ca2+ signaling. A symposium in Hamburg, Germany, brought together 120 researchers from various fields, who were all engaged in the molecular characterization of the key players of NAD+ signaling (www.NAD2008.de).. ...

Cadp-ribose/ACM119340535 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.

A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS ...

This multiunctional enzyme catalyses both the synthesis and hydrolysis of cyclic ADP-ribose, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 2.4.99.20, 2-phospho-ADP-ribosyl cyclase/2-phospho-cyclic-ADP-ribose transferase. cf. EC 3.2.2.5, NAD+ glycohydrolase ...

یک تیم از دانشمندان به رهبری دانشگاه ناگویا در ژاپن شناسایی کرده بسیار غیر معمول اتمی پیکربندی در یک تنگستن-بر اساس مواد. تا به حال اتمی پیکربندی کرده و دیده می شود در trihydrogen یک یون وجود دارد که در بین سیستم های ستاره ای در فضا است. یافته های منتشر شده در مجله Nature Communicationsنشان می دهد مطالعات بیشتر می تواند آشکار ترکیبات با جالب خواص الکترونیکی.. اتم را تشکیل می دهند که انسان ها و درختان و جداول آشپزخانه به طور کلی پیوند با یکدیگر با به اشتراک گذاشتن الکترون - فکر می کنم از الکترون ها از اتمی به عنوان چسب زندگی است. دانشگاه ناگویا اعمال فیزیکدان Yoshihiko Okamoto و ...

Kurose Hitomi , Okamoto Mayumi , Shimizu Miyuki , BITO Takaaki , MARCELLE Cristophe , NOJI Sumihare , OHUCHI Hideyo Development, growth & differentiation 47(4), 213-223, 2005-06-01 参考文献35件 被引用文献2件 ...

Viavi Solutions Koji Okamoto discusses how the company is adapting its testing products to meet the challenges of Remote PHY and Fiber Deep.

Okamoto, M., Iguchi, T., Hattori, T., Matsuzaki, S., Koyama, Y., Taniguchi, M., Komada, M., Xie, M. J., Yagi, H., Shimizu, S., Konishi, Y., Omi, M., Yoshimi, T., Tachibana, T., Fujieda, S., Katayama, T., Ito, A., Hirotsune, S., Tohyama, M. & Sato, M., 18-02-2015, In: Journal of Neuroscience. 35, 7, p. 2942-2958 17 p.. 研究成果: Article › 査読 ...

Hypothalamic oxytocin (OT) is released into the brain by cyclic ADP-ribose (cADPR) with or without depolarizing stimulation. Previously, we showed that the intracellular free calcium concentration ([Ca2+]i) that seems to trigger OT release can be elevated by -NAD+, cADPR, and ADP in mouse oxytocinergic neurons. As these -NAD+ metabolites activate warm-sensitive TRPM2 cation channels, when the incubation temperature is increased, the [Ca2+]i in hypothalamic neurons is elevated. However, it has not been determined whether OT release is facilitated by heat in vitro or hyperthermia in vivo in combination with cADPR. Furthermore, it has not been examined whether CD38 and TRPM2 exert their functions on OT release during stress or stress-induced hyperthermia in relation to the anxiolytic roles and social behaviors of OT under stress conditions. Here, we report that OT release from the isolated hypothalami of male mice in culture was enhanced by extracellular application of cADPR or increasing the

Signaling dinucleotides: The first single-isomer synthesis of nicotinamide adenine dinucleotide phosphate (NADP) is reported. Installation and maintenance of sensitive phosphate and pyridinium functionalities were key to success. Significantly, conversion of NADP into the important mammalian second

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The other principal source of Ca2+ for signalling is the internal stores that are located primarily in the endoplasmic/sarcoplasmic reticulum (ER/SR), in which inositol-1,4,5-trisphosphate receptors (IP3Rs) or ryanodine receptors (RYRs) regulate the release of Ca2+. The principal activator of these channels is Ca2+ itself and this process of Ca2+-induced Ca2+ release is central to the mechanism of Ca2+ signalling. Various second messengers or modulators also control the release of Ca2+. IP3, which is generated by pathways using different isoforms of phospholipase C (PLCbeta, delta, epsilon, gamma and zeta), regulates the IP3Rs. Cyclic ADP-ribose (cADPR) releases Ca2+ via RYRs. Nicotinic acid adenine dinucleotide phosphate (NAADP) may activate a distinct Ca2+ release mechanism on separate acidic Ca2+ stores. Ca2+ release via the NAADP-sensitive mechanism may also feedback onto either RYRs or IP3Rs. cADPR and NAADP are generated by CD38. This enzyme might be sensitive to the cellular metabolism, ...

A Membrane-bound or cytosolic enzyme that catalyzes the synthesis of Cyclic ADP-Ribose (cADPR) from Nicotinamide Adenine Dinucleotide (NAD). This enzyme generally catalyzes the Hydrolysis of cADPR to ADP-Ribose, as well, and sometimes the synthesis of Cyclic ADP-Ribose 2 phosphate (2-P-cADPR) from NADP ...

As the result of successful collaboration with Dr. K. Mikoshibas laboratory in RIKEN Brain Science Institute, Tokyo, Japan, we found that pancreatic protease activation by alcohol metabolite mainly depends on Ca2+ release via acid store IP3 receptors (Gerasimenko J. et al, PNAS, 2009). Currently there is no specific pharmacological treatment for pancreatitis. However, now our research has identified the critical proteins responsible for the excessive calcium release which is where the problem begins with the possibility to search for specific chemical compounds for the treatment of acute pancreatitis.. I am investigating the action of nicotinic acid adenine dinucleotide phosphate (NAADP), a novel Ca2+ releasing messenger and its role in the induction of pathological processes of exocrine pancreas. Our findings (Gerasimenko J, et al., JCS, 2006) show that the NAADP-sensitive Ca2+ pool is located in the endoplasmic reticulum and in acidic organelles, which are represented by secretory granules, ...

Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal and endosomal membrane.

Increasing evidence has indicated that NAD+ and NADH play critical roles not only in energy metabolism, but also in cell death and various cellular functions including regulation of calcium homeostasis and gene expression. It has also been indicated that NAD+ and NADH are mediators of multiple major biological processes including aging. NAD+ and NADH produce the biological effects by regulating numerous NAD+/NADH-dependent enzymes, including dehydrogenases, poly(ADP-ribose) polymerases, Sir2 family proteins (sirtuins), mono(ADP-ribosyl)transferases, and ADP-ribosyl cyclases. Of particular interest, NAD+-dependent generation of ADP-ribose, cyclic ADP-ribose and O-acetyl-ADP-ribose can mediate calcium homeostasis by affecting TRPM2 receptors and ryanodine receptors; and sirtuins and PARPs appear to play key roles in aging, cell death and a variety of cellular functions. It has also been indicated that NADH and NAD+ can be transported across plasma membranes of cells, and that extracellular NAD+ ...

Social Mobilizer Jobs in Pakistan for the month of September 2021. You can find Social Mobilizer Jobs online at JobStock in Pakistan.

Zhumadilov, Kassym; Ivannikov, Alexander; Stepanenko, Valeriy; Zharlyganova, Dinara; Zhumadilov, Zhaxybay; Apsalikov, Kazbek; Toyoda, Shin; Zhumadilova, Anara; Endo, Satoru; Tanaka, Kenichi; Miyazawa, Chuzou; Yamamoto, Masayoshi; Okamoto, Tetsuji; Hoshi, Masaharu; ...

Tao Okamoto is a gown wearing diva for the December issue of Numéro China. Photographed by John-Paul Pietrus and styled by Tim Lim, the Japanese model is full of attitude ...

Okamoto, Jun*; Mamiya, Kazutoshi*; Fujimori, Shinichi; Okane, Tetsuo; Saito, Yuji; Muramatsu, Yasuji*; Yoshii, Kenji; Fujimori, Atsushi*; Tanaka, Arata*; Abbate, M.*; et al.. Physical Review B, 71(10), p.104401_1 - 104401_5, 2005/03. ...

Nicotinic acid adenine dinucleotide phosphate (NAADP) has recently been shown to act as a second messenger controlling intracellular Ca responses in mammalian cells. Many questions remain regarding this signaling pathway, including the role of the ryanodine receptor (RyR) in NAADP-induced Ca transients. Furthermore, the exact metabolic pathway responsible for the synthesis of NAADP in vivo has not been determined. Here, we demonstrate that the NAADP mediated Ca release system is present in human myometrial cells. We also demonstrate that human myometrial cells use the NAADP second messenger system to generate intracellular Ca transients in response to histamine. It has been proposed in the past that the NAADP system in mammalian cells is dependent on the presence of functional RyRs. Here, we observed that the histamine-induced Ca transients are dependent on both the NAADP and inositol 1,4,5-trisphosphate signaling pathways but are independent of RyRs. The enzyme CD38 has been shown to catalyze ...

Mutations in LRRK2 (leucine-rich repeat kinase 2) represent a significant component of both sporadic and familial PD (Parkinsons disease). Pathogenic mutations cluster in the enzymatic domains of LRRK2, and kinase activity seems to correlate with cytotoxicity, suggesting the possibility of kinase-based therapeutic strategies for LRRK2-associated PD. Apart from cytotoxicity, changes in autophagy have consistently been observed upon overexpression of mutant, or knockdown of endogenous, LRRK2. However, delineating the precise mechanism(s) by which LRRK2 regulates autophagy has been difficult. Recent data suggest a mechanism involving late steps in autophagic-lysosomal clearance in a manner dependent on NAADP (nicotinic acid-adenine dinucleotide phosphate)-sensitive lysosomal Ca2+ channels. In the present paper, we review our current knowledge of the link between LRRK2 and autophagic-lysosomal clearance, including regulation of Ca2+-dependent events involving NAADP.

Tsuchiya, T and Okamoto, K, The relationship between the oxygen consumption of various tissues and the radiosensitivity in mice. I. Oxygen consumption of various tissues in the normal physiological state of mice. (jap.) (1965). Subject Strain Bibliography 1965. 974 ...

The IUPHAR/BPS Guide to Pharmacology. ADP ribose ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.

Kondo-Ando, M., Seino, Y., Morikawa, R., Negi, K., Todoroki, H., Kawakami, T., Asada, Y., Yoshimoto, R., Tanaka, C., Okamoto, K., Masuda, A., Tomatsu, E., Hiratsuka, I., Yoshino, Y., Maki, W., Kakita, A., Shibata, M., Takayanagi, T., Makino, M., Sugimura, Y. および7人, Asai, S., Ito, A., Ueno, S., Fujiwara, Y., Kuwata, H., Yabe, D. & Suzuki, A., 11-2019, ： : Journal of Diabetes and its Complications. 33, 11, 107415.. 研究成果: Article ...

Affiliation：福井大学,学術研究院医学系部門(附属病院部),講師, Research Field：Otorhinolaryngology, Keywords：鼻科学,浸潤,ケモカイン,DEPs,スギ花粉症,内分泌撹乱物質,STAT6,血管新生,IgE,酸化ストレス, # of Research Projects：4, # of Research Products：6, Ongoing Project：遺伝子導入による顔面神経軸索再生の試み

Freitas, H. S., Okamoto, M. M., David Silva, A., Sabino-Silva, R., Furuya, D. T., Souza, M. O. de, & Machado, U. F. (2010). O envolvimento da AKT na regulação da transcrição gênica de GLUT2 e SGLT2 em rim de diabéticos, via insulina e o fator transcricional HNF-3β. In Resumos. São Paulo: FeSBE ...

Freitas, H. S., Okamoto, M. M., David Silva, A., Sabino-Silva, R., Furuya, D. T., Souza, M. O. de, & Machado, U. F. (2010). O envolvimento da AKT na regulação da transcrição gênica de GLUT2 e SGLT2 em rim de diabéticos, via insulina e o fator transcricional HNF-3β. In Resumos. São Paulo: FeSBE ...

Its our favourite excuse to stay in on a Saturday night, but one we might not be able to use this year - ITV are said to be considering postponing the...

Ca 2+ signaling in spermatozoa plays a crucial role during processes such as capacitation and release of the acrosome, but the underlying molecular mechanisms still remain unclear. Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca 2+ -releasing second messenger in a variety of cellular processes. The presence of a NAADP synthesizing enzyme in sea urchin sperm has been previously reported, suggesting a possible role of NAADP in sperm Ca 2+ signaling. In this work we used in vitro enzyme assays to show the presence of a novel NAADP synthesizing enzyme in human sperm, and to characterize its sensitivity to Ca 2+ and pH. Ca 2+ fluorescence imaging studies demonstrated that the permeable form of NAADP (NAADP-AM) induces intracellular [Ca 2+ ] increases in human sperm even in the absence of extracellular Ca 2+ . Using LysoTracker®, a fluorescent probe that selectively accumulates in acidic compartments, we identified two such stores in human sperm cells. Their acidic nature was further

A Membrane-bound or cytosolic enzyme that catalyzes the synthesis of Cyclic ADP-Ribose (cADPR) from Nicotinamide Adenine Dinucleotide (NAD). This enzyme generally catalyzes the Hydrolysis of cADPR to ADP-Ribose, as well, and sometimes the synthesis of Cyclic ADP-Ribose 2 phosphate (2-P-cADPR) from NADP ...

Antony Giuseppe Galione (born 1963) FRS FMedSci is professor of Pharmacology and Wellcome Trust Senior Investigator in the Department of Pharmacology at the University of Oxford. Galione was educated at Felsted School in Essex and Trinity College, Cambridge where he was awarded a Bachelor of Arts degree in Natural Sciences (Pharmacology) in 1985 followed by a PhD in 1989 for research on calcium signalling in the blowfly salivary gland supervised by Michael Berridge. Galiones research investigates calcium signalling. He established the concept of multiple calcium mobilising messengers which link cell surface stimuli to release of internal calcium stores, and identified their target two-pore channels (TPCs) and organelles. This has enhanced our understanding of how calcium as a ubiquitous cellular regulator may control a myriad of cellular processes with precision. He established that cyclic ADP-ribose regulates calcium-induced calcium release and globalisation of calcium signals, and that ...

A chemo-enzymatic synthesis of novel caged NAADP+ without the formation of multiple cage compounds has been achieved. The biological activity of the caged NAADP+ was demonstrated by its fast uncaging in intact sea-urchin eggs.

Game Republic (Folklore) founder Yoshiki Okamoto is a game development legend, having created titles such as Time Pilot, 1942, and Street Fighter II -

TY - JOUR. T1 - Potential role of the CD38/cADPR signaling pathway as an underlying mechanism of the effects of medetomidine on insulin and glucose homeostasis. AU - Guedes, Alonso Gp. AU - Rude, Elaine P.. AU - Kannan, Mathur S.. PY - 2013/9. Y1 - 2013/9. N2 - Objective: To investigate the CD38/cADPR signaling pathway as possible underlying mechanism of the effects of medetomidine on insulin and glucose homeostasis. Animals: Thirty-two C57BL/6 mice of both sexes. Methods: Wild-type (WT) and CD38-knockout (CD38-/-) mice received medetomidine (50 μg kg-1) or a similar volume of 0.9% NaCl (control) by intraperitoneal (IP) injection (each group n = 8). The mice were euthanized 45 minutes later with sodium pentobarbital IP and blood was sampled via cardiac puncture. Insulin and glucose concentrations were measured by radioimmunoassay and by the oxygen rate method, respectively. Data were analyzed with anova and Bonferroni post hoc (5% significance) and are shown as mean ± SD. Results: Plasma ...

Affiliation：島根大学,医学部,准教授, Research Field：Pathological medical chemistry,Hematology,Cardiovascular medicine,Emergency medicine,Urology, Keywords：血管内皮細胞,トロンボモジュリン,炎症,ギャップ結合,コネキシン,プロテインCインヒビター,プロテインC,活性化プロテインC,プロテインS,血液凝固, # of Research Projects：14, # of Research Products：122, Ongoing Project：腹部臓器大網乳班が分泌するトレロソームが敗血症による免疫麻痺を引き起こす

東京大学大学院新領域創成科学研究科 国際交流室の公式サイト。国際交流室の紹介、入試情報など。

Celebrity is a fickle thing. Some who have it prefer to hide - the Kevin Spaceys, Dave Chapelles and Doris Days of the world - while others seem to relish the spotlight - the Kanye Wests, Joseph…

Keiko Okamoto, Norihito Emura, Hajime Sato, Yuki Fukatsu, Mitsuru Saito, Chie Tanaka, Yukako Morita, Kayo Nishimura, Eriko Kuramoto, Dong Xu Yin, Kazuharu Furutani, Makoto Okazawa, Yoshihisa Kurachi, Takeshi Kaneko, Yoshinobu Maeda, Takashi Yamashiro, Kenji Takada, Hiroki Toyoda and Youngnam Kang ...

The Robert and Donna Heider Engineering Scholarship is available to full - time undergraduate students enrolled in the University of Missouri-St. Louis/Washington University joint engineering progr...