I am planning on doing a cyanogen bromide digest of huamn serum albumin next week and I was looking for some pointers from anyone with CNBr digest experience. 1. The method I found calls for dissolving the protein in 70% formic acid, adding solid CNBr (2 mg CNBr/mg protein), and then letting react for 24h at RT in the dark. Is this a reliable and efficient procedure? 2. If I try the CNBr digest on HSA without first reducing the disulfides, will the CNBr cleave or modify the disulfides? 3. If I do reduce the disulfides of HSA but do not alkylate before the CNBr digest, will the cysteines end up alkylated (to the isothiocyanate)? Any help would be appreciated! -David ...
Studies were made to characterise soluble Alfa-Amylase (Bacterial). The kinetics of Alfa-amylase on starch is zero order at room temperature (28 0 c) for the first 5 minutes. Hence the activity of Alfa-amylase was measured in terms of mg maltose released during first five minutes. Alfa-amylase showed optimal activity at pH 6.0 Cyanogen bromide was prepared and was used to activate sephadex G200 at pH 11.5. The volume of the cyanogen bromide activated gel, at this pH decreased by about 50 percent, compared to that of the unactivated Sephadex G200. Alfa-amylase was coupled to cyanogen bromide activated Sephadex G200 at pH8.3 and 7.0. Coupling of the enzyme led to further decrease in volumes of about 15 percent and 6 percent at pH 8.3 and 7.0 respectively. The amounts of protein in the immobilized Alfa-amylase prepared at pH 8.3 and 7.0 were estimated by, Kjeldhal method, by the tryptophan content and from the difference in the amount of protein present in the original solution and that in the ...
Many cyanogen bromide cleavage protocols fail to cleave ubiquitin because of its stability in acid. The following protocol has proved effective for cleaving the ubiquitin mutant I36W P37M F45W. ...
Lookchem Provide Cas No.506-68-3 Basic information: Properties,Safety Data,Sds and Other Datebase. We also Provide Trading Suppliers & Manufacture for 506-68-3 Cyanogen bromide.
The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
We couldnt find a conversion between grams Cyanogen Chloride and nanomol Do a quick conversion: 1 grams Cyanogen Chloride = 1 nanomol using the online calculator for metric conversions.
Absolute spectrophotometry of the coma of Comet Kohoutek 1973f is discussed for the nights of January 24 and 26, 1974. Specific intensities are measured for spectral features and a continuum band in the wavelength region λλ3460-6062Å. The (0, 0) band of the ∆ν = 0 sequence of the violet system of the cyanogen molecule is analyzed and column densities of 1.7 × 10 |SUP|15|/SUP|m |SUP|-2|/SUP| and 3.4 × 10 |SUP|14|/SUP|m |SUP|-2|/SUP| are found for January 24 and 26, 1974, respectively. The analysis of the bands of C|SUB|2|/SUB| will be reported in a second paper of this series.
It is not difficult to immobilize a protein on a solid support using any classic activation method such as the cyanogen bromide or N-hydroxysuccinimidylcarboxylate... However, if the protein of interest is a specific antibody, its biological activity will be affected if not entirely lost after the covalent linkage between the solid support with the active site H2N of the antibody.. AFFILAND has developed an Antibody Activated Binding Gel for immobilizing the antibody at its Fc portion leaving free its Fab. The biological properties of immobilized antibody will be so conserved.. II. PURIFICATION OF ANTIGEN BY CORRESPONDING IMMUNOAFFINITY COLUMN ...
It is not difficult to immobilize a protein on a solid support using any classic activation method such as the cyanogen bromide or N-hydroxysuccinimidylcarboxylate... However, if the protein of interest is a specific antibody, its biological activity will be affected if not entirely lost after the covalent linkage between the solid support with the active site H2N of the antibody.. AFFILAND has developed an Antibody Activated Binding Gel for immobilizing the antibody at its Fc portion leaving free its Fab. The biological properties of immobilized antibody will be so conserved.. II. PURIFICATION OF ANTIGEN BY CORRESPONDING IMMUNOAFFINITY COLUMN ...
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TY - JOUR. T1 - Characterization of the cyanogen bromide fragments of the β chain of human haptoglobin. AU - Kurosky, Alexander. AU - Hay, Regine E.. AU - Kim, Han Hwa. AU - Touchstone, Billy. AU - Rasco, Marilyn A.. AU - Bowman, Barbara H.. PY - 1976. Y1 - 1976. N2 - Characterization of the cyanogen bromide (CNBr) fragments of the β chain of human haptoglobin revealed five major fragments resulting from cleavage of four methionyl residues. The fragments were isolated by gel filtration in guanidine-HCl on Sepharose 6B and Bio-Gel P10 and P60. Compositional analyses of the five cyanogen bromide fragments accounted for 248-253 amino acid residues in agreement with the number of residues determined for the intact β chain. Most of the carbohydrate was attached to CNBr II. Automated amino-terminal sequence analysis and carboxyl-terminal hydrolysis with carboxypeptidase of the haptoglobin β chain and cyanogen bromide fragments identified 139 residues, or about 55% of the β-chain molecule. The ...
Cyanogen was first synthesized in 1815 by Joseph Louis Gay-Lussac, who determined its empirical formula and named it. Gay-Lussac coined the word cyanogène from the Greek words κυανός (kyanos, blue) and γεννάω (gennao, I create), because cyanide was first isolated by the Swedish chemist Carl Wilhelm Scheele from the pigment Prussian blue.[14] By the 1850s, cyanogen soap was used by photographers to remove silver stains from their hands.[15] It attained importance with the growth of the fertilizer industry in the late 19th century and remains an important intermediate in the production of many fertilizers. It is also used as a stabilizer in the production of nitrocellulose. In 1910 a spectroscopic analysis of Halleys Comet found cyanogen in the comets tail, which led to public fear that the Earth would be poisoned as it passed through the tail. Because of the extremely diffuse nature of the tail, there was no effect when the planet passed through it.[16][17]. ...
TY - JOUR. T1 - Implementation of new TPD analysis techniques in the evaluation of second order desorption kinetics of cyanogen from Cu(001). AU - Ciftlikli, Erkan Z.. AU - Lee, Everett Y.M.. AU - Lallo, James. AU - Rangan, Sylvie. AU - Senanayake, Sanjaya D.. AU - Hinch, B. J.. PY - 2010/12/21. Y1 - 2010/12/21. N2 - The interactions of cyanide species with a copper (001) surface were studied with temperature programmed desorption (TPD) and X-ray photoelectron spectroscopy (XPS). Adsorbed cyanide species (CN(a)) undergo recombinative desorption evolving molecular cyanogen (C2N 2). As the adsorbed CN species charge upon adsorption, mutually repulsive dipolar interactions lead to a marked desorption energy reduction with increasing CN(a) coverages. Two new TPD analysis approaches were developed, which used only accurately discernible observables and which do not assume constant desorption energies, Ed, and pre-exponential values, ν. These two approaches demonstrated a linear variation of Ed with ...
Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. - Antibody Purification (Affinity) - AbVideo™ - Support - Abnova
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Aldrich-53272; Hexyltrimethylammonium bromide |=98.0% (AT); CAS Number: 2650-53-5; Linear Formula: CH3(CH2)5N(Br)(CH3)3; find related products, papers, technical documents, MSDS & more at Sigma-Aldrich.
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The amino acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan has been determined. The very basic protein, by itself a moderately neurotoxic phospholipase, consists of a single polypeptide chain of 119 amino acids. The main fragmentation of the reduced and S-carboxymethylated derivative was accomplished by cleavage with Staphylococcus aureus V8 protease and trypsin. Chymotryptic peptides and cyanogen bromide fragments were used to align and complete the sequence, which was determined by automated Edman degradation. The taipoxin alpha-subunit is closely homologous to the other taipoxin subunits and to other elapid snake venom phospholipases A2.
Complete amino acid sequences were determined for thymopoietins I and II (revision), isolated from bovine thymus, and for thymopoietin III, a newly identified polypeptide isolated from bovine spleen. Thymopoietin III (TP-III) is a 49 amino acid monomeric peptide that shows minor microheterogeneity at residue 34. The three thymopoietins have largely identical sequences yet some distinct differences, suggesting very recent evolution from a common gene. The complete amino acid sequences are (Formula: see text). ...
In article ,CKBILy.7pG at ucdavis.edu, szsclark at hamlet.ucdavis.edu (Sonya Clark) writes: ,Dear Netters - I am purifying antibodies from a polyclonal prep by ,running them over a CNBr-activated Sepharose affinity column of my ,antigen. As CNBr-activated Sepharose is expensive &/or horrible to make, Well it isnt that expensive compared to the time saved by buying it (unless youre a graduate student whose time isnt valued much) and it isnt all that bad to make (N-methyl-pyrrolidone/Na2CO3 method ) if you have a fume hood but anyhow ,Id like to strip the column of the antigen currently bound, and re-use ,the sepharose to make another affinity column. the real point is that the activated bond from the CNBr is used up by the coupling procedure. Even if you could get the protein off, youre back to unactivated sepharose. Joe Mack mack at ncifcrf.gov Does anyone have a protocol ,to do this? Would eluting the column with harsh eluents (eg. ,Guanidine/urea/SDS) work? and would I then be able to ...
Cellular responses of adhesion, spreading, or migration to adhesive GPs are mediated by multiple recognition domains of individual molecules.33 35 Many adhesive GPs involved in cell-cell and cell-matrix interactions, including fibrin(ogen), have both HBDs8 9 10 11 12 13 14 15 16 17 18 and RGD cell binding domains.32 33 34 36 37 38 39 Therefore, fibrin solubilized by CNBr cleavage was used to explore the structural requirements of binding to cultured EC monolayers and to study the potential involvement of both RGDS- and heparin-mediated interactions in this binding. Because the coiled-coil region imparts structural rigidity to fibrin(ogen) molecules,40 we postulated that limited cleavage within this region would aid in retention of the conformational integrity of the cryptic β15-42 HBD.18 Therefore, partial CNBr cleavage of methionine residues was performed to produce large-molecular-weight but soluble fragments of fibrin (Fig 2⇑). ELISA and Western blot analysis of CNBr fragments of FBG and ...
Cyanogen halides are employed for binding water-soluble proteins and water-soluble peptides containing a primary or secondary amino group to a water-insoluble polymer containing one or more hydroxyl and/or primary and/or secondary amino groups. The invention is useful, among other things, for binding watersoluble enzymes to water-insoluble polymers while preserving the activity of the enzyme.
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CAS NO:896465-66-0; Chemical name:1-Isoamyl-2-methyl-3,3-dibenzyl-5-chloroindolium bromide ; physical and chemical property of 896465-66-0, 1-Isoamyl-2-methyl-3,3-dibenzyl-5-chloroindolium bromide is provided by ChemNet.com
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Optimized PM6_D3H4 data set: MERS=(1,2,1) GNORM=4 PM6-D3H4 THREADS=1 Titanium(iv) bromide (TiBr4) (PM6-D3H4) Ti 0.42698700 +1 0.2117731 +1 0.8921759 +1 Ti -4.30491892 +1 -6.4773070 +1 -1.5004360 +1 Ti 0.29021966 +1 -0.9009843 +1 -4.2307153 +1 Br -3.69888946 +1 -1.8617788 +1 -7.1002692 +1 Br -1.83235907 +1 0.6018724 +1 1.8313998 +1 Br -6.38944936 +1 -2.5674896 +1 1.9787709 +1 Br 1.33312170 +1 -2.1289795 +1 -6.6325740 +1 Br -4.52669224 +1 -1.2562574 +1 -4.8394398 +1 Br 0.38164887 +1 2.5009449 +1 -0.5997460 +1 Br -2.95861573 +1 -8.5301858 +1 -0.6240677 +1 Br -1.81455526 +1 -2.2341104 +1 -3.7158022 +1 Br -1.96759876 +1 -4.9482290 +1 -2.3612729 +1 Br -0.93164843 +1 -0.0371416 +1 -1.6955077 +1 Br -4.34518515 +1 -2.7016668 +1 -1.9973386 +1 Br 1.63487108 +1 -0.1297490 +1 -1.5933006 +1 Br -6.60795364 +1 -3.6162984 +1 -4.6166030 +1 Br 3.06474099 +1 0.2288122 +1 1.4148497 +1 Br -2.55751279 +1 -5.1568974 +1 0.0759385 +1 Br -0.79366532 +1 0.7826121 +1 -6.3379464 +1 Ti -3.72372204 +1 -3.8230296 +1 -4.4825364 ...
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