Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran province. The polyclonal antibodies were used to the samples by DAS-ELISA . Some of the tested samples, showed positive reaction against cucumber mosaic virus (CMV) specific antibody. Some of the positive samples which are mechanically inoculated on test plants.Some leaf samples from mint plants with symptoms of yellows and mosaic were showed positived reaction against cucumber mosaic virus specific antibody.To confirm the identify of the virus detected using a specific primer pair of CMV which amplify part of coat protein of CMV . A fragment about 700bp was amplified in Immunocapture reverse transcriptase polymerase
TY - JOUR. T1 - Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein. AU - Kim, Min Jung. AU - Kim, Hwa Ran. AU - Paek, Kyung Hee. PY - 2006/11/1. Y1 - 2006/11/1. N2 - The cucumber mosaic virus (CMV) replication complex has previously been shown to associate with cellular membranes. However, it remains unknown whether any host factors participate in this process. In this study, five groups of Arabidopsis tonoplast intrinsic protein (TIP) genes were isolated and the proteins they encoded were evaluated with regard to their interactions with CMV proteins. TIP1 and TIP2 were found to interact with the CMV 1a protein in the Sos recruitment system, whereas no interactions with the other three TIP subgroups were observed in this assay. The interaction of CMV 1a with the TIP1 and TIP2 proteins was confirmed via co-immunoprecipitation assays. Additionally, CMV 1a co-localized with TIP1 and TIP2 in transfected Arabidopsis protoplasts. The findings of ...
Pseudorecombinant and chimeric recombinant strains have been created between two strains of cucumber mosaic virus (I17F-CMV and R-CMV) or between R-CMV and tomato aspermy virus (P-TAV), and their...
Keywords: POSTDOCTORAL POSITION ANNOUNCEMENT IN MOLECULAR EVOLUTION OF PLANT VIRUSES PROJECT DESCRIPTION: Applicants are being sought to fill TWO positions in molecular evolution of plant viruses. Position one will involve molecular population biology, epidemiology, and evolutionary mechanisms of peanut stunt cucumovirus. Several virus collections are already available for this study. Position two will involve a study of 1.) the role of viral and host components of the replicase in the generation of variability, and 2.) the role of quasispecies in the biology of cucumber mosaic cucumovirus, including the ability to overcome resistance. More detailed descriptions of both projects are available on request. REQUIREMENTS: A Ph.D. in Molecular Biology, Biochemistry, or related field, with experience in evolution or virology helpful. SALARY and APPOINTMENT: Base salary is US$24,000/year, depending on experience with funding available for 3 years. STARTING DATES: July and August 1995 APPLY TO: Dr. ...
Courgette plants are also susceptible to Cucumber Mosaic Virus, but dont throw the plant away until you are sure. As stated before I would expect to see puckered, mottled leaves that turn yellow with time. The whole plant will have stunted growth. Does your corgette plant have these symptoms? Tell me about where you are growing it indoors, if it is still alive I would certainly plant it outside now as all danger of frost has passed, it may be protesting at the fact that it is still indoors! Was the plant siiting in a saucer of water? Was enough air getting to the roots or were they possibly waterlogged? Was the plant sat on a very hot and sunny windowsill where the young leaves were getting scorched? The more information you can give me, the more chance I have of solving this ...
A woodiness disease of passionfruit was described by Cobb (1901) but as both passionfruit woodiness and cucumber mosaic viruses may cause woody fruit and leaf mosaic (Taylor, 1959), the authenticity of records prior to that of McKnight (1953) is in doubt. However, passionfruit woodiness virus was rare in P. edulis in Queensland in 1927 and widespread in 1932 (Simmonds, 1959) suggesting that it was introduced into that State about 1927. Its absence from cool, temperate areas of Australia and the rare occurrence of cucumber mosaic virus in Queensland suggest that ecological factors influence the distribution of both viruses. Experimentally, a mixed infection by the two viruses in P. edulis causes a very severe disease (Taylor, 1959), but naturally occurring mixed infections are not always so virulent. To differentiate the two viruses it is best to use the electron microscope; the filamentous particles of passionfruit woodiness virus are usually readily found in the sap of infected plants. The ...
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When New Yorks snap bean growers began suffering widespread devastating losses, Stephen Reiners, Cornell University horticulture professor, assembled a "research village" to develop short-term and long-term responses. The multidisciplinary task force included Cornell horticultural specialists, entomologists, a seed scientist, a plant pathologist, a plant breeder, a virologist and extension educators.. "At the start, we looked at the fields with severe yield loss and had more questions than answers," Reiners says. "We identified the problem as cucumber mosaic virus and called together the expertise to answer our questions. It was a full effort to respond quickly.". Julie Kikkert, Cornell Cooperative Extension vegetable specialist, served as the conduit to the processing industry and the growers who periodically met with the research team.. Pete Call was among the growers whose fields were 50 to 100 percent infected with cucumber mosaic virus (CMV) in 2001. Call, who has 2,500 acres of beans, ...
Find out all you need to about caring for Wisteria. Read advice from RHS to find out everything from growing your own plants, fruit & veg to winter pruning.
Symptoms on plants infected with cucumoviruses can vary. Generally, plants appear lighter in color and are bushy and stunted. Close up symptoms include a mosaic (alternating light and dark green areas) on at least some leaves, especially on the younger leaves. Leaves may exhibit a shoestringlike appearance. Fruit production is greatly reduced. These symptoms can be confused with those caused by tobacco mosaic virus. ...
Gene silencing suppressor 2b complexed with silencing RNA (ribonucleic acid). Computer model showing the structure of the gene silencing suppressor 2b (purple, green) complexed with synthetic silencing RNA (yellow, magenta). From Tomato aspermy virus. - Stock Image C035/8421
To determine the structural requirements for cucumber mosaic virus (CMV) satellites to elicit lethal tomato necrosis, three satellite variants D, S and Y were used in the construction and cloning of chimeric cDNAs. D and S are necrogenic and non-necrogenic prototype variants, respectively, and Y possesses the 3′ conserved necrosis-determining region but does not cause lethal tomato necrosis. Its 5′ half harbours an insertion/deletion region that results in a molecule about 30 nucleotides longer than other variants. Tomato bioassays were conducted with RNA transcripts of all six chimeric combinations of the 5′ and 3′ halves of the three satellite variants divided by a common restriction site, as well as with a mutated chimera. None of the chimeras containing the 5′ half of Y induced lethal necrosis in tomato even when their 3′ halves were that of the D variant with the conserved necrogenic element. Chimeras with the 3′ half of Y elicited only partial or restricted necrosis which was much
The Cucumber mosaic virus (CMV)-encoded 2b protein (Cmv2b) is a nuclear protein that suppresses transgene RNA silencing in Nicotiana benthamiana. Cmv2b is an important virulence determinant but nonessential for systemic spread in N. glutinosa, in contrast to its indispensable role for systemic infections in cucumber. Here, we report that Cmv2b became essential for systemic infections in older N. glutinosa plants or in young seedlings pre-treated with salicylic acid (SA). Expression of Cmv2b from the genome of either CMV or Tobacco mosaic virus significantly reduced the inhibitory effect of SA on virus accumulation in inoculated leaves and systemic leaves. A close correlation is demonstrated between Cmv2b expression and a reduced SA-dependent induction of the alternative oxidase gene, a component of the recently proposed SA-regulated antiviral defense. These results collectively reveal a novel activity of Cmv2b in the inhibition of SA-mediated virus resistance. We used a N. tabacum line ...
The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (CMVD2b) induced strong resistance to aphids (Myzus persicae) while CMV infection fostered aphid survival. Using electrical penetration graph methodology we found that higher proportions of aphids showed sustained phloem ingestion on CMV-infected plants than on CMVD2b-infected or mock-inoculated plants although this did not increase the rate of growth of individual aphids. This indicates that while CMV infection or certain viral gene products might elicit aphid resistance, the 2b protein normally counteracts this during a wild-type CMV infection. Our findings suggest that the 2b protein could indirectly ...
Normand, Russell Anthony, "Studies on the Differential Transmission of Strains of Cucumber Mosaic Virus by Aphids." (1967). LSU Historical Dissertations and Theses. 1356 ...
The NBRP is one of the research arms of NARO, the National Banana Research Program is mandated to carry out research on Banana in Uganda.
Satellite RNAs Cucumber mosaic virus (an epiphytotic of lethal necrotic disease among tomato plants in France in 1972). This was unusual because of the severity and atypical nature of the symptoms associated with infections of tomato plants (See description of cucumber mosaic in prior text). Enhanced response of tomatoes to this disease were associated with host used for virus propagation; virus grown in tobacco or tomato yielded an enhanced necrotic response, those propagated in cucurbit hosts yielded a reduce necrotic response. RNA extractions of necrotic tomato plants revealed the presence of variable amounts of a small RNA species in addition to the expected three genomic and one subgenomic RNAs. This 5th RNA species was designated CARNA 5 (i.e., CMV-associated RNA 5 or CMV satRNA), a satellite RNA. When CARNA 5 is present with CMV, symptoms of the disease are enhanced in tomato plants, while in tabasco pepper the presence of CARNA 5 tends to attenuate the disease symptoms caused by CMV. ...
Satellite RNAs Cucumber mosaic virus (an epiphytotic of lethal necrotic disease among tomato plants in France in 1972). This was unusual because of the severity and atypical nature of the symptoms associated with infections of tomato plants (See description of cucumber mosaic in prior text). Enhanced response of tomatoes to this disease were associated with host used for virus propagation; virus grown in tobacco or tomato yielded an enhanced necrotic response, those propagated in cucurbit hosts yielded a reduce necrotic response. RNA extractions of necrotic tomato plants revealed the presence of variable amounts of a small RNA species in addition to the expected three genomic and one subgenomic RNAs. This 5th RNA species was designated CARNA 5 (i.e., CMV-associated RNA 5 or CMV satRNA), a satellite RNA. When CARNA 5 is present with CMV, symptoms of the disease are enhanced in tomato plants, while in tabasco pepper the presence of CARNA 5 tends to attenuate the disease symptoms caused by CMV. ...
Courgette Defender F1. Resistant to cucumber mosaic virus, the cause of many crop failures. Very early, with uniform dark green fruits. Crops all summer.
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Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli).
fr] Actuellement, dans lest de la Chine, le contrôle des pucerons repose sur lutilisation systématique dinsecticides de synthèse. Lanalyse de la structure socio-économique des ménages ruraux de la province du Shandong a permis de mettre en avant la méconnaissance des agriculteurs vis-à-vis des effets néfastes de ces molécules chimiques sur lenvironnement et sur la santé humaine. Cette conclusion conduit à rechercher de nouvelles méthodes alternatives efficientes pour les producteurs chinois. Dans ce contexte de lutte biologique contre les pucerons et leur transmission virale (Potato virus Y (PVY), Cucumber mosaic virus (CMV) et Potato Leaf Roll Virus (PLRV)), trois substances sémiochimiques à savoir, l(E)-β-farnésène, le cis-3-hexényl acétate, lextrait dail et une culture intercalaire de pois ont été expériméntées séparément à laide de pièges jaunes dans des cultures de pommes de terre et de courgettes. Entre ces diverses modalités, aucune différence ...
Figure 8 shows the overall quaternary structure derived for the AMV particles. The VRU strain differs only slightly in amino acid sequence from that of AMV 425 (Fig. , 1979). Nevertheless, the VRU strain is also capable of forming the stacked lattice, reflecting the polymorphism of the assembled protein. The proposed hexagonal surface lattice possesses twofold symmetry axes andd quasi-three- and quasi-sixfold axes. Two types of dimer interactions are in evidence, one producing a flat face (a dihedral angle of 180° between trimers) while the other utilizes a dihedral angel of about 140°, similar to that observed between trimers of an icosahedron. However, there is no serological relationship between AMV and TSV or CVV or CLRV and the tryptic peptide maps of TSV and AMV protein show no similarity (van Vloten-Doting, 1975). Capsid proteins from the Bromo- and Cucumovirus groups will not activate Ilarvirus RNA. Recently, Roosien and van Vloten-Doting (1983) reported a coat protein mutant of AMV ...
I think I used about 12 cucumbers but you can pickle any amount, just adjust the quantity of spiced vinegar you make. The night before, peel and slice all your cucumbers and place in a large bowl. Pour over about a tablespoon of salt. Dont worry, you wont eat the salt. If you dont drawn the fluid from the cucumbers it dilutes the vinegar too much in the jars. Mix the cucumbers around with your hands, making sure the salt is well distributed, put a clean tea towel over the top of the bowl and leave it overnight. The following morning there will be a lot of fluid in the cucumber bowl. Pour it all into a colander and let the salty water go down the drain. Run clean cold water from the tap over the cucumbers to make sure all the salt is removed. Leave the cucumber in the colander for about an hour to drain ...
How to Make Cucumber Juice. Cucumber juice is a very healthy and versatile drink. Cucumbers have a high content of water and contain noteworthy amounts of potassium, silica, vitamin A, vitamin C, folate, and chlorophyll, among other...
Cucumbers are known to mankind for over six thousand years. Article about calories in cucumber, useful properties of cucumberm how to choose them.
Because cucumbers are mostly water, they are low in calories -- even a large cucumber contains less than 100 calories -- but they still come loaded with...
Cucumber nutrition one of the oldest cultivated crops. It has a lot of benefits. Know more about cucumber nutrition in this article.
It was recently discovered that the widespread plant pathogen cucumber mosaic virus elevates the release of host volatiles, or odorous chemicals, that attract vectors. Researchers measured the rates of aphid population growth on and emigration from healthy and infected plants. It was discovered that aphids were initially more attracted to the infected plants than to the healthy plants. However, the aphids dispersed rapidly from the diseased hosts after feeding. This form of transmission is known as non-persistent transmission because rather than long-term feeding and colonizing on the plants (persistent), the vectors are repelled by the inferior quality and move on to other plants (non-persistent). This type of transmission is advantageous for CMV as it facilitates easy transmission from one host to another ...
This project applies biotechnology to effectively mitigate the impact of pests and pathogens on priority floral crops and develops systems that can be manipulated to preserve select hardwood tree taxa for future genetic engineering applications. Genetic engineering of floral crops for pest and pathogen resistance contributes to sustainable production. There has been little research done on migratory nematodes such as Pratylenchus, and this project addresses the use of transgenes that may be effective in controlling Pratylenchus. Viruses are always a problem for propagated plants, particularly flower bulb crops that are propagated from the same bulb for many years. This project determines if an RNAi approach is effective for control of Cucumber mosaic virus (CMV), an economically important virus that affects numerous crops. In vitro manipulation of plants through genetic engineering allows for rapid clonal propagation, ploidy level manipulation, and preservation of germplasm and cryopreservation. ...
2020) LreEF1A,, a translation elongation factor from Lilium regale, Is pivotal for cucumber mosaic virus and tobacco rattle virus infections and tolerance to salt and drought. International Journal of Molecular Sciences, 21 (6). Article 2083. Jia, Y., Selva, C., Zhang, Y., Li, B., McFawn, L.A., Broughton, S., Zhang, X., Westcott, S., Wang, P., Tan, C., Angessa, T., Xu, Y., Whitford, R. and Li, C. ...
Slicing cucumbers are best served fresh and raw, but they can also be sautéed, fried, or even mixed in drinks. Cucumbers contain most of their nutrients in their seeds and their skin. It is advised, from a nutritional standpoint, to eat cucumbers with both. However, cucumbers have wax coatings on their skin. Organically grown cucumbers have non-synthetic waxes where conventionally grown cucumbers may have synthetic waxes and pesticides. If you choose to eat the skin, buy organic cucumbers to eliminate the risk of synthetic wax consumption. If buying organic is not an option, then thoroughly wash each cucumber with a brush or simply remove the skin before eating.. ...
when they are about 6-8 inches long. Cucumbers intended for dill pickles can be harvested at 4 to 6 inches long and pickles at as little as 2 inches. The larger burpless cucumbers can be up to 10 inches long and some types are even larger, although if they are left on the vine too long, they can become bitter or yellow. The highest quality cucumber is uniformly green, firm and crisp.. At peak harvesting time, you should be picking cucumbers every couple of days. Pay attention to what the plant is telling you. Check plants daily, ensuring no fruit is laying on the ground, rotting and that the tendrils and leaves are growing where you want them. Training cucumber vines and picking them as they ripen will ensure longer and healthy vegetable production.. As cucumbers are over 90% water, they must be stored tightly in plastic wrap and properly refrigerated. They will keep (on average) 7-10 days when properly stored and delicious in dozens of recipes and applications.. We certainly have enjoyed ...
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In this chapter, students explore the history of the bitter gene in cucumbers, which was found in a genebank and traded internationally between cucumber breeders. The students then explore transcription, translation, and the DNA basis for different types of mutations. This activity uses one of the modern tools of genomics-sequences from the GenBank public database-to explore mutation of cucumber genes.. ...
When choosing cucumbers, Korean cucumbers are ideal for their soft skin and crunch, but pickling cucumbers are a good substitute. This refreshing instant kimchi is wonderful with barbecued meats and a glass of beer.
Health Benefits of Cucumber - Cucumber is a vegetable which is known for its mild and pleasant watery taste. It is a highly cultivated vegetable and is...
Cucumbers are the fourth most cultivated vegetable in world and known to be one of the best foods for bodys overall health. Cucumber and ayurveda: When we analyze properties of cucumber based on ayurvedic principles, it pacifies pitta dosha. Pitta dosha is associated with heat. Pitta when aggravated can cause…
To make a cucumber detox drink, mix 8 cups of water with a medium-sized cucumber, a lemon and around 10 mint leaves. Let the mixture steep in the refrigerator overnight before you drink it....
With its cooling, hydrating effect, cucumber is excellent for the skin. Learn how to nourish and sooth with cucumber homemade skin care.
In recent times slowly but surely we have seen a rise in the number of cucumber flavored drinks that are on the market. Its nowhere near the...
The investigation of pests and diseases that contribute to reduced productivity of hot peppers has been ongoing. In Belize, baseline data collected on major pests in Cayo and Belize districts showed that white flies (Hemiptera: Aleyrodidae) and cucumber beetle (Acalymma vittatum (Fabricius) and Diabrotica undecimpunctata howardi Barber) were the most prevalent pests. Virus infection is still a major problem for hot pepper production. Screening of hot pepper fields in Barbados for Potato Virus Y (PVY) and Cucumber Mosaic Virus (CMV) revealed that 60% of the samples were positive for CMV and 78% for PVY. A survey of common weeds in the hot pepper fields showed many species testing positive for the viruses. Future studies will investigate weed seeds as sources for transmission of the viruses. Meanwhile, the results suggest that field sanitation will be important in managing the viruses ...
Diseases caused by aphid-transmitted viruses such as Alfalfa mosaic virus (AMV) and Cucumber mosaic virus (CMV) have increased in snap bean (Phaseolus vulgaris) in the Midwestern United States. Plants immediately surrounding agricultural fields may serve as primary virus inocula for aphids to acquire and transmit to bean crops.
Plant Disease, Volume 96, Issue 4, Page 506-514, April 2012.http://dx.doi.org/10.1094/PDIS-02-11-0089 | Plant health
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Cucumbers are rich in two of the most basic elements needed for healthy digestion: water and fiber. Adding cucumbers to your juice or salad can help you meet the ideal of amount of fiber your body needs - 50 grams per 1,000 calories consumed. If you struggle with acid reflux, you should know that drinking water can help suppress acute symptoms of acid reflux by temporarily raising stomach pH; its possible that water-rich cucumbers may have a similar effect ...
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Cucumbers contain three different types of lignans which reduce the risk of cardiovascular disease. Try Cucumber Avocado Rolls for some added benefit!