To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...

BACKGROUND: Cryopreserved human sperm are used in assisted reproductive technology. However, the effect of cryopreservation on sperm DNA integrity is unclear. OBJECTIVES: The objectives of this study were to: (i) determine the impact of semen cryopreservation on human sperm DNA integrity and chromatin structure; (ii) test if parameters obtained from TUNEL and SCSA® correlate; and (iii) verify correlation between sperm motility, morphology and viability with TUNEL and SCSA® parameters. MATERIALS AND METHODS: Men attending a fertility clinic were recruited and grouped according to their sperm parameters (n = 9/group): normozoospermia, oligoasthenoteratozoospermia and teratozoospermia. Each semen sample was processed as follow: (i) directly frozen at -80 °C; (ii) diluted in Sperm Maintenance Medium, cooled for 30 min at 4 °C and frozen at -80 °C; (iii) diluted in Sperm Maintenance Medium; or (iv) in SpermFreeze. Each mixture from method (iii) and (iv) was then suspended for 30 min in liquid nitrogen

Cryopreservation is the preservation of cells, tissues or even organs at very low temperatures, with the intention of future use. At the temperature of -196oC, metabolism stops and the cells enter a state of suspended animation making it possible to store them for a long time. To achieve such a low temperature we use liquid nitrogen. The first successful cryopreservation was performed in frogs in 1945. The first animal sperm banks operated in 1949. The first successful inseminations I the human were reported in 1953 (preservation in dry ice, -70oC) and in 1964 in liquid nitrogen. Embryo cryopreservation was first performed in laboratory animals in 1972 and in human in 1984.. Today, cryopreservation is a routine method, allowing the storage of gametes and embryos for an extended period.. ...

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Whenever a sudden shift is made from fresh embryo transfer to frozen embryo transfer the couple usually have many queries in mind. So, lets evaluate both and see pros and cons.. Fresh embryos transfer is undergoing ovarian hyper stimulation making of embryos and transferring resultant embryos to uterine cavity in same cycle. In frozen embryo transfer first two steps of controlled ovarian hyper stimulation and process of IVF/ICSI are same but the resultant embryos are frozen using vitrification techniques and not transferred in same cycle. They are thawed and then kept uterine cavity after preparing the uterine cavity. In patients who are normal or hyper responders the frozen embryo transfers are known to have better results than the fresh embryo transfer . The reason can be that due to ovarian hyper stimulation and high levels of estrogen may have negative impacts on the endometrial receptivity. In some cycles another hormone called progesterone may also be raised, which again decreases embryo ...

TY - JOUR. T1 - The bioenergetics of mitochondria after cryopreservation. AU - Fuller, Barry J.. AU - Rubinacci, Alessandro. AU - Geboes, Karel. AU - De Loecker, William. PY - 1989. Y1 - 1989. N2 - The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.. AB - The functional characteristics ...

Sometimes couples undergoing in vitro fertilization (IVF) need more than a single cycle to conceive. In recent years, frozen embryo transfers (FET) has become a popular option before moving to a fresh IVF cycle. FET at PRC allows you to extend the chance of pregnancy per egg retrieval, saving you time and money.. FROZEN EMBRYO TRANSFER (FET). A frozen embryo transfer (FET) is a cycle in which the frozen embryos from a previous fresh IVF or donor egg cycle are thawed and then transferred back into the womans uterus.. Lower Cost. The cost of frozen egg transfer is significantly lesser than a fresh cycle of IVF. This combination of reduced cost and equal success rates makes frozen embryo transfers an exciting option over fresh IVF cycle at the top IVF & egg fertility clinic in Los Angeles.. Less Medication. In a fresh cycle, you would again need to take stimulation medication. On the other hand FET IVF & egg fertility clinic in Los Angeles just requires you to use estrogen and progesterone to ...

A cohort of 91 children from cryopreserved embryos and 83 control children who were conceived normally had their development assessed using the Griffithss scales of mental development. The controls (81 singletons and two twins) of a similar age, sex, and social class were selected from siblings, cousins, and peers of the cryopreserved embryo group (68 singleton, 20 twins, and three triplets). Children from cryopreserved embryos had a lower mean birth weight and mean gestational age and a higher proportion were born by caesarean section. One child from the cryopreserved embryo group had Downs syndrome, three had squints, and four had conductive hearing loss while in the control children, six had squints, and nine had conductive hearing loss. In both groups, including the child with Downs syndrome, the mean Griffithss quotient was greater than the standard 100. In the children from cryopreserved embryos, the singleton and multiple birth subgroups had statistically similar assessment results. ...

TY - JOUR. T1 - Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. AU - Jin, Shiying. AU - Lei, Lei. AU - Shea, Lonnie D.. AU - Zelinski, Mary B.. AU - Stouffer, Richard L.. AU - Woodruff, Teresa K.. N1 - Funding Information: Supported by Oncofertility Consortium: National Institutes of Health grants RL1-HD058295 and PL1EB008542 and Training for a New Interdisciplinary Research Workforce (T90) grants 1TL1CA133837 , U54-HD18185 , and NCRR RR00163 . PY - 2010/5/15. Y1 - 2010/5/15. N2 - Objective: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. Design: Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. Setting: University-affiliated laboratory. Animals: Rhesus monkey ovarian tissue. Intervention(s): None. Main Outcome Measure(s): Histologic analysis, follicle counting, ...

BACKGROUND: Vanilla siamensis is listed in Appendix-II of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) as an endangered species in Thailand. OBJECTIVE: To develop an optimum cryopreservation protocol for V. siamensis. MATERIALS AND METHODS: Protocorms were precultured on solid ½ MS medium with 0.5 M sucrose for 0-7 d. For encapsulation-dehydration, encapsulated protocorms (beads) were dehydrated for 0-6 h. In the case of encapsulation-vitrification, the beads were loaded with a plant vitrification solution 2 (PVS2) at 0°C for 0-90 min. RESULTS: Protocorms precultured for 3 d gave the highest post-cryopreservation survival of 17%. Dehydration of the encapsulated protocorm beads for 4 h gave the highest survival of 33% and a regrowth of 25%. Protocorms subjected to the encapsulation- vitrification method did not survive at all. CONCLUSION: Protocorms precultured with 0.5 M sucrose for 3 d, encapsulated with 3% sodium alginate and dehydrated to a ...

According to a recent report from the HFEA features a pattern towards Assisted Reproductive Technology (ART) enlistments across the globe. It states that frozen embryo transfer has been increasingly seen as a mainstream embryo transfer protocol.. HFEA measurements depict that fresh embryo transfers dropped by 11 percent somewhere in the range of 2013 to 2018. On the other hand, frozen embryo transfers nearly multiplied, representing 38% of all In Vitro Fertilization (IVF) cycles in 2018.. At one center, frozen embryo banks outperformed intercontinental donations as a treatment for donors from upcoming years after 2014. Hence, of the 1283 donor cycles finished somewhere in the range of 2005 to 2013. Out of these, 88 percent were treated as treatments from other states, yet today 95% of egg donors have moved to frozen donor eggs, in egg bank.. Also, the pattern of blastocyst transmission in some centers mirrors a similar trend now, that is currently clear in the developing enlistment information: ...

Both proceedures have seen great success rates in the last five years and thousands of baby boys and girls have been born using this technology.. Endocrine Abstracts says, Semen cryopreservation is a viable fertility preservation option for adolescent cancer patients, and should be offered to all before treatment, acknowledging the caveat of a ~50% chance of success. Pubertal staging is the only significant prognosticator of this and should be routinely assessed as part of the counselling process.. While the ethics surrounding fertility preservation in younger cancer patients are being debated, research is ongoing to take fertility tissue from patients where eggs and sperm are not necessarily matured or viable.. Speak to your health professional about fertility preservation options they may be aware of. The two of you will be able to decide if the process is right for you and decide on your next steps.. If youre wary of fertility options, remember, Geoff has managed to father two beautiful ...

Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the ...

There is a lump sum due at the time of cryopreservation. This pays for the cost of transportation, cryopreservation protocols, and also for ongoing costs of maintenance for the cryonics patient. This lump sum is normally covered with a dedicated life insurance policy.. Cryonics organizations also have membership dues, which are separate from the cost of the cryonics life insurance. There are no additional costs due once a member is in cryopreservation.. ...

Cryopreservation is a reliable means for the long-term conservation of plant genetic resources. It is of particular interest for cocoa (Theobroma cacao L.) whose seeds are recalcitrant to conventional storage methods and field collections susceptible to disease infestations. However, the encapsulationdehydration procedure previously developed for cocoa somatic embryos has resulted in poor survival after retrieval from liquid nitrogen. To examine the causes of such failure, cocoa somatic embryos following each treatment step of the encapsulation-dehydration procedure were examined using a combination of confocal scanning laser microscopy and transmission electron microscopy. Results showed that the parenchymas cells of the hypocotyl and radicle were the major sites of injury possibly due to their large size and non-cytoplasmic nature, whereas the shoot meristem and provascular strand were well preserved throughout the treatments. In general, cell deformation and/or disruption was observed following the

The summer flounder, Paralichthys dentatus L., is a high-value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose-based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (?5, ?10 and ?15°C min?1) were evaluated using differential staining to assess post-thaw sperm survival. Seven combinations of the factors examined reduced post-thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO) frozen at ?5°C min?1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical

Principles of Cryopreservation and Optimization of Vitrification This talk discusses about the principles of using cryoprotectants to achieve cryopreservation including slow freezing and vitrification. Based on the principles, several approaches used to improve vitrification are reviewed. The future directions of the c

Despite of important advances in the knowledge of the reproductive biology of several deer species (mainly, red deer), there is a lot to investigate. We are trying to answer a practical and a basic question: how can we improve sperm cryopreservation in cervids? and, which underlying, cellular and molecular, changes occur during cooling and cryopreservation?. These are important questions not only for our Iberian deer species (Iberian red deer and roe deer), but also to endangered cervids elsewhere in the world (specially South-American species such as the huemul and pudu). Technology transfer from well-known species to related but less studied species might improve their conservation.. ...

TY - JOUR. T1 - Ovine semen cryopreservation usingthree extenders and four combinations of permeant and non permeantagents. AU - Rocío Sandoval, M.. AU - Alexei Santiani, A.. AU - Luis Ruiz, G.. AU - Víctor Leyva, V.. AU - Luis Coronado, S.. AU - Alfredo Delgado, C.. PY - 2007/1/1. Y1 - 2007/1/1. N2 - © 2007 Universidad Nacional Mayor de San Marcos. All rights reserved. The objective of the study was to evaluate the effect of three extenders and four combinations of two permeant and two non permeant cryoprotectant agents on the quality of post thaw ram semen. In Experiment 1, three extender were evaluated (A, B, and C) in order to select the best for the next step. In Experiment 2, different combinations of cryoprotectant agents were evaluated as follow: 1) Glycerol-Trehalose, 2) Glycerol- Sucrose, 3) Ethylene glycol-Trehalose, and 4) Ethylene glycol-Sucrose. In experiment 1, motility, viability and acrosomal integrity in extender A were higher than in extender B and extender C, and ...

Hepatocytes are an important physiological model for evaluation of metabolic and biological effects of xenobiotics. They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large quantities of healthy cells that maintain high hepatospecific functions. In culture, the morphology of hepatocytes cryopreserved with wheat protein extracts (WPE) was similar to that of fresh cells. Furthermore, hepatospecific functions such as albumin secretion and biotransformation of ammonium to urea were well maintained during 4 days in culture. Inductions of CYP1A1 and CYP2B in hepatocytes cryopreserved with WPEs were similar to those in fresh hepatocytes. These findings clearly show that WPEs are an excellent

IVF and ICSI are fully established methods of assisted reproduction which can be used for patients awaiting cytotoxic therapy:. Following the data of the German, Swiss and Austrian network Fertiprotekt (www.fertiprotekt.eu), 164 of 1388 counselled patients have chosen ovarian stimulation and cryopreservation of oocytes as a fertility preservation technique in 2007 2009. Among those patients 2417 oocytes were collected (Mean: 11.8, Range 0 41, STD 7.3). In 125 patients oocytes were fertilized and cryopreserved, resulting in an fertilisation rate of 70.5%/aspirated oocyte. Only in one patient, chemotherapy needed to be postponed due to severe ovarian hyperstimulation syndrome.. These data reveal that ovarian stimulation can result in adequate numbers of oocytes. However, they also demonstrate, that this technique is not successful in all patients. Combination with cryopreservation of ovarian tissue should therefore be considered. FertiPROTEKT performed a study in which 50% of one ovary was removed ...

Guidelines contents   Introduction   Recommendations   Discussing fertility   Management   Impact   Options  

Poster (2015, June 14). Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]. Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, ...

TY - JOUR. T1 - Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. AU - Coticchio, G.. AU - De Santis, L.. AU - Rossi, G.. AU - Borini, A.. AU - Albertini, D.. AU - Scaravelli, G.. AU - Alecci, C.. AU - Bianchi, V.. AU - Nottola, S.. AU - Cecconi, S.. PY - 2006/7. Y1 - 2006/7. N2 - Background: Recently described slow-cooling cryopreservation protocols involving elevated sucrose concentration have improved survival frequencies of human oocytes, potentially overcoming a major hurdle that has limited the adoption of oocyte storage. Because implantation rates of embryos from frozen oocytes remain generally low, it is still debated whether, irrespective of survival rates, this form of cryopreservation leads inevitably to the disruption or complete loss of the metaphase II (MII) spindle. Methods: Human oocytes with an extruded polar body I (PBI) were cryopreserved using a slow-cooling method including 1.5 ...

This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important little details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL ...

By Leigh E. Towill (auth.), Dr. Leigh E. Towill, Prof. Dr. Y. P. S. Bajaj (eds.). Ex situ upkeep of germplasm for better plant species has been accom- plished utilizing both seeds or clones, yet garage of those less than commonplace condi- tions doesnt give you the severe longevities which are had to reduce danger of loss. expenditures of upkeep and regeneration of shares also are excessive. platforms that supply almost indefinite garage should still complement current tools and its inside this context that cryopreservation is gifted. using low temperature upkeep was once firstly extra a priority of drugs and animal breeding, and used to be multiplied to crops within the Nineteen Seventies. Sur- vival after cryogenic publicity has now been validated for various plant teams together with algae, bryophytes, fungi and better vegetation. If survival is com- monplace, then the eventual program is a cryopreservation process, wherein cells, tissues and organs are held indefinitely to be used, usually ...

The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly alter the proportion of positively stained cells for CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells. A small but statistically

Research from many laboratories over the past several decades indicates that invertebrate oocytes and eggs are extraordinarily difficult to freeze. Since starfish oocytes, eggs, and embryos are an important cell and developmental biology model system, there is great interest to cryopreserve these cells. Previous starfish oocyte cryopreservation studies using slow cooling protocols revealed that these cells are highly sensitive to osmotic stress and form intracellular ice at very high sub-zero temperatures, suggesting that common freezing methodologies may not prove useful. We report here that a short exposure to 1.5 M Me2SO/1 M trehalose in hypotonic salt solution followed by ultra-rapid cooling to cryogenic temperatures allows starfish oocytes to be cryopreserved with the average survival rate of 34% when normalized to control oocytes that were exposed to CPA, but not frozen. On average, 51% of the oocytes in 77% of the batches of frozen oocytes underwent meiotic maturation in response to the ...

Since the discovery of Vitrification in 2008, the pregnancy rate of frozen embryos is exactly the same or, in some cases, higher than that of fresh embryos transferred after In Vitro Fertilisation. Pregnancy rates are five times higher than the gestation rates of intrauterine insemination, which is why frozen embryo transfer is currently one of the most effective and safest techniques to welcome a baby home.. Frozen embryo transfer is the most effective and safest technique if we want to achieve a full-term pregnancy in cases of infertility or desire to be a mother. Pregnancy rates are related to the age of the couple, especially the mother, since it clearly accentuates the possibility of gestation.. ...

Egg freezing and cryopreservation services provided by Dr. Akin at Bluegrass Fertility Center located in Lexington, KY. Other services include IVF, Assisted Hatching, ICSI and PGD.

Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to Show moreCryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the ...

The delivery rate achieved after the vitrification of early cleavage- and blastocyst-stage embryos is not affected by the embryo developmental stage or any other variable related to the warming cycle.

Frozen embryo transfers have higher success rates than fresh embryo transfers. Within frozen embryo transfers there are natural and programmed cycles. Each have tradeoffs in terms of cost, intensity, drug requirements and more.

Results of this study10 were surprising, to say the least. Without going into the specifics of different types of fertility preservation and their respective indications, less than half of the respondents (45.6%) reported being aware of fertility preservation. Specialists in O&G fared no better in this regard with only half (50.7%) of the respondents reporting themselves as being aware. As expected, O&G specialists were more aware of fertility-preservation techniques in females such as oocyte- and embryo-freezing as well as ovarian tissue freezing, than their non-O&G counterparts. Interestingly, when respondents were further asked about individual fertility-preservation procedures, an increased awareness was found. In fact, a higher percentage of the same O&G specialists in this study reported to be familiar with all of the above fertility-preservation techniques previously itemised, compared with being aware of fertility preservation per se (63.6% vs 50.7%). These findings highlight a ...

Ovarian tissue cryopreservation (OTC) is offered to women treated for acute leukemia to preserve their fertility before hematopoietic stem cell transplantation. The risk of leukemic infiltration in ovarian samples harvested before administration of chemotherapy limits ovarian tissue transplantations. We assessed the minimal residual disease (MRD) by sensitive quantitative polymerase chain reaction in cryopreserved ovarian cortex and medulla samples harvested from 30 patients in complete remission of acute leukemia, including 60 % with negative bone marrow MRD at the time of OTC. Ovarian MRD was undetectable in 21 patients (70%), detectable below 10-4 in 8 patients (27%) and between 10-3 and 10-4 in 1 patient (3%). Twenty patients (67%) had concordant MRD between bone marrow and ovarian samples. Interestingly 4 patients had positive MRD in ovarian samples while undetectable in bone marrow. Our results underline the importance of reaching the best control of the disease with undetectable or low ...

Mary Zelinski, PhD finishes her reports from the annual meeting of the Society for Cryobiology held from Corvallis, Oregon with a final blog about the keynote

Knowing that there are options for many women to protect or preserve their reproductive potential before cancer treatment begins can make it less challenging to discuss the possible reproductive side effects of treatment with your patients

TY - JOUR. T1 - Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - DeHoyos, Tenok O.. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. N1 - Funding Information: This research was supported by grants from the National Institute of Health / National Institute of Biomedical Imaging and Bioengineering , R01 EB008388 , and the National Science Foundation , CBET-1009465 . PY - 2011/8. Y1 - 2011/8. N2 - The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In ...

Not all frozen embryo transfer cycles and protocols are the same; however, I am sharing my FET cycle to provide insight into how FET cycles can go.

The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format. By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. To select a subset of the search results, click Selective Export button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export. After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format. ...

The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was ...

What are benefits of frozen embryo transfer? Before the advent of embryo freezing , surplus embryos had to be discarded after fresh embryo transfer. Read on to find out more

PubMed journal article: Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Download Prime PubMed App to iPhone, iPad, or Android

FERRARI, Edilene Aparecida Preti; COLOMBO, Ronan Carlos; FARIA, Ricardo Tadeu de and TAKANE, Roberto Jun. Criopreservação de sementes de Encholirium spectabile Martius ex Schultes f. pelo método da vitrificação. Rev. Ciênc. Agron. [online]. 2016, vol.47, n.1, pp.172-177. ISSN 0045-6888. http://dx.doi.org/10.5935/1806-6690.20160020.. A bromélia Encholirium spectabile Martius ex Schultes f. é uma espécie endêmica da Caatinga. O objetivo deste trabalho foi avaliar a eficiência de soluções crioprotetoras em sementes de bromélia. Os tratamentos consistiram da imersão das sementes em soluções crioprotetoras e de vitrificação, antes da imersão em nitrogênio líquido (NL) (-196 ºC), conforme os tratamentos a seguir: T1 - controle: sem crioprotetores; T2 - glicerol 2M (20 min) + PVS2 (10 min); T3 - glicerol 2M (20 min) + PVS2 com floroglucinol a 1% (10 min); T4 - sacarose 0,4M (20 min) + PVS2 (10 min); T5 - sacarose 0,4M (20 min) + PVS2 com floroglucinol a 1% (10 min); T6 - glicerol ...

The Fertility Preservation Program also will endeavor to aid women and girls who are starting cancer treatment or preparing for a bone marrow transplant. Currently their choices are quite limited and, for many, biologically impossible or morally unacceptable, noted Joseph S. Sanfilippo, M.D., director of the Center for Fertility and Reproductive Endocrinology at Magee-Womens Hospital.. Women can undergo ovarian stimulation to generate multiple mature eggs, which can then be fertilized with partner or donor sperm to produce embryos for later implantation and possible pregnancy, he said. These techniques are now well-established, and we will offer them to female patients who wish to pursue this option. Another option is ovarian cryopreservation, where an ovary is removed before cancer therapy is initiated with the plan to replace ovarian tissue once the patient is cured.. Still, the stimulation technique takes time and could delay cancer treatment. It also produces high estrogen levels that ...

Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets. PDLSC sheets prepared from extracted human molars were divided into two groups: the cryopreservation group (cPDLSC sheets) and the freshly prepared control group (fPDLSC sheets). The cPDLSC sheets were cryopreserved in a solution consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide for 3 months. Cell viability and cell proliferation rates of PDLSCs in both groups were evaluated by cell viability assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. The multilineage

In previous years of in-vitro fertilization research, it was thought that fresh embryo transfers were superior to frozen embryo transfers and that frozen embryo transfers should be avoided and only used when there was no other option. New research in recent years however has changed the data on fresh and frozen embryo transfer cycles and the effectiveness of each in terms of pregnancy and live birth rates. This has in turn impacted many providers decisions to perform a freeze-all delayed embryo transfer versus a fresh embryo transfer.. Many people may become confused about the terminology regarding frozen embryo transfers. A freeze-all approach is where all embryos from the resulting IVF cycle are cryopreserved (frozen) and a fresh embryo transfer is not attempted until a later date. This is different from freezing extra or leftover embryos which are the result following a fresh IVF transfer.. The three main things that affect the pregnancy and live birth rate following IVF treatment are ...

Pioneering work by Quinn and Burrows in the late 1930s led to successful artificial insemination (AI) programs in the domestic poultry industry. A variety of species specific modifications to the Quinn and Burrows massage technique made AI possible in nondomestic birds. Massage semen collection and insemination techniques span the entire range of species from sparrows to ostriches. Also, cooperative semen collection and electroejaculation have found limited use in some nondomestic species. Artificial insemination produces good fertility, often exceeding fertility levels in naturally copulating populations. However, aviculturists should explore other ways to improve fertility before resorting to AI. Artificial insemination is labor intensive and may pose risks to nondomestic birds as well as handlers associated with capture and insemination. Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. Yet, semen cryopreservation techniques need improvement before fertility

Fertility Preservation Options for Females with Turner Syndrome Are Growing: A new article by Dr. Oktay and colleagues reviews options and provides guidelines.

A novel model capable of quantitatively describing and predicting Intracellular Ice Formation (IIF) as a function of temperature in a cell population during the cooling stage of a cryopreservation protocol, without Cryo-Protective Agent (CPA) is proposed. The model accounts for water osmosis and IIF occurrence during freezing of the cell population, whose size distribution dynamics is simulated by means of a suitable population balance approach. It is found that IIF temperature depends upon the cell size, i.e. it is higher for larger cells. Correspondingly, the Probability of IIF (PIIF) results to be dependent on the initial size distribution of the cell population. Model reliability is successfully verified by predicting experimental data available in the literature of PIIF at different, constant cooling rates with better accuracy as compared to previous theoretical approaches.. ...

TY - JOUR. T1 - Live birth rates with a freeze-only strategy versus fresh embryo transfer. T2 - secondary analysis of a randomized clinical trial. AU - Vuong, Lan N.. AU - Pham, Toan D.. AU - Dang, Vinh Q.. AU - Ho, Tuong M.. AU - Ho, Vu N.A.. AU - Norman, Robert J.. AU - Mol, Ben W.. PY - 2019/3. Y1 - 2019/3. N2 - Research question: What are the roles of serum progesterone and endometrial thickness as biomarkers in the decision between a freeze-only and fresh embryo transfer in IVF for women without polycystic ovary syndrome (PCOS)? Design: This was a secondary analysis of a randomized controlled trial including 782 couples who were followed up until the end of the first completed cycle. Couples scheduled for their first or second IVF cycle with a FSH/gonadotrophin-releasing hormone antagonist protocol were randomized to a freeze-only (n = 391) or fresh embryo transfer (n = 391) strategy. The endpoint for this analysis was live birth rate (LBR) after the first embryo transfer. Results: There ...

Vollmer, R.; Panta, A.; Tay, D.; Roca, W.; Ellis, D. 2014. Effect of preculture and PVS2 exposure on the cryopreservation of sweet potato shoot tips [Ipomoea batatas (L.) Lam.] using the PVS2 droplet vitrification. In: Reed, B.M. (ed). Proceedings of the Second International Symposium on Plant Cryopreservation. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 265-271 ...

Prostasomes are extracellularly occurring organelles which are secreted in human semen by the prostate gland. Prostasomes have several known biological activities, but their physiological function is still unclear. In this thesis some new aspects were studied on the biological role of the prostasomes. The motility-stimulatory effect of prostasomes on cryopreserved spermatozoa was further studied by supplementing the swim-up medium with seminal prostasomes, and with prostasomes purified from a PC-3 prostate cancer cell line (PC-3 prostasomes), on fresh spermatozoa. The recovery of motile spermatozoa after swim-up increased by 50% when the swim-up medium was supplemented with prostasomes. The PC-3 prostasomes bore a functional resemblance to seminal prostasomes as regards various expressions of sperm motility promotion. Prostasomes proved to have potent antibacterial effects. The effects were not strictly confined to Bacillus megaterium since a few other bacteria were also sensitive. The high ...

The aim of this study was to investigate the impact of TSH levels on clinical outcomes 14 days after frozen-thawed embryo transfer. Blood samples were collected on the first visit to our department and 14 days after embryo transfer. Women were divided into three groups based on D14 TSH levels, which were compared to basal TSH levels in groups with different clinical outcomes. TSH levels between pregnant and nonpregnant women were also compared. The clinical pregnancy rate in women with lower TSH levels 14 days after transfer was slightly but significantly lower (56%, P = 0.05) compared to those with higher TSH levels. Furthermore, TSH levels were significantly elevated 14 days after transfer compared to basal TSH levels in pregnant women and in women who successfully became pregnant (P | 0.001, respectively). Elevated TSH levels 14 days after embryo transfer compared to basal TSH levels seem to play a protective role and predict favorable clinical outcomes under specific conditions.

TY - JOUR. T1 - Follicular development, ovulation, and corpus luteum formation in cryopreserved human ovarian tissue after xenotransplantation. AU - Kim, S. Samuel. AU - Soules, Michael R.. AU - Battaglia, David E.. PY - 2002/7/15. Y1 - 2002/7/15. N2 - Objective: To assess the competency of human frozen/thawed ovarian follicles matured in xenografts to form functioning corpora luteae after human chorionic gonadotropin (hCG) administration. Design: Prospective controlled animal study. Setting: University research laboratory. Patient(s): Three women (19, 28, and 36 years) who underwent oophorectomy. Animal(s): Nineteen female severe combined immunodeficient (SCID) mice. Intervention(s): Cryopreserved human ovarian tissues were grafted into the s.c. space of bilaterally oophorectomized SCID mice. All the animals were stimulated with pregnant mares serum gonadotropin (PMSG) for 4 weeks starting from 16 weeks after transplantation. Twelve animals were injected with hCG at the end of gonadotropin ...

Fertility Preservation in Adults with Cancer Agenda Background information The effects of cancer treatments on fertility Fertility preservation options Referral and patient experience  Study conducted to explore attitudes, knowledge & referring behaviors in Oncofertility among Ontario physicians providing adult cancer care  Survey sent to 641 physicians in Ontario (medical oncologists, radiation oncologists, gynecologic oncologists & urologists)  152 physicians returned the survey - response rate of 23.7% Results: 1. Majority of oncologists had positive attitudes but many did not have current knowledge of cryopreservation 2. Great majority did not have educational materials or patient resources to facilitate discussion 3. ~25% did not know where to refer male cancer patients for sperm banking; almost 50% rarely made a referral 4. ~ 45% did not know where to refer female cancer patients for fertility preservation consultation; 70% rarely made a referral Results: • Study conducted to ...

Very limited information on the post-implantatory effects of vitrification has been published till now. We observed in a previous study that the vitrification procedure for the cryopreservation of embryos introduced transcriptomic and proteomic modifications in the rabbit foetal placenta at the middle of gestation. Now, we have conducted a proteomic study to determine whether protein alterations in the foetal placenta induced by the vitrification procedure remain during pregnancy. In this study, we used 2D-DIGE and mass spectrometry (MALDI-TOF-TOF and LC-MS/MS analysis) to identify the protein changes during middle and late stages of gestation (Day 14 and Day 24, respectively) in rabbit foetal placenta. We identified 11 differentially expressed proteins at Day 14 and 13 proteins at Day 24. Data are available via ProteomeXchange with identifiers PXD001840 and PXD001836. In addition, we demonstrate the presence of three proteins, serum albumin, isocitrate dehydrogenase 1 [NADP+], and ...

Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used for rational design of CPA equilibration procedures, the necessary permeability data is extremely scarce for adherent cells. This study validates a method for in situ measurement of water and CPA permeability in adherent cells based on the fluorescence quenching of intracellular calcein. Permeability parameters for endothelial monolayers were measured during exposure to four common cryoprotectants (dimethyl sulfoxide, ethylene glycol, propylene glycol and glycerol) at temperatures of 4°C, 21°C and 37°C. Propylene glycol exhibited the highest permeability and glycerol the lowest. The data was fit using an Arrhenius model, yielding activation ...

Looking for online definition of Cryostorage in the Medical Dictionary? Cryostorage explanation free. What is Cryostorage? Meaning of Cryostorage medical term. What does Cryostorage mean?

Cryopreservation of tissue slices greatly facilitates their use in drug metabolism research, leading to efficient use of human organ material and a decrease of laboratory animal use. In the present review, various mechanisms of cryopreservation such

This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the Entrepelado and Lampiño breeds. The samples were suspended in a commercial extender and refrigerated to 17 degrees C for transport to the laboratory (step A), where they were further extended with a lactose-egg yolk-based extender and chilled to 5 degrees C (step B) prior to freezing in the presence of glycerol (3%). Spermatozoa were assessed for plasma membrane integrity and sperm motility at each of the steps, including post-thaw (step C). Aliquots were also prepared for immunocytochemical localisation of the sugar transporters (fixed and thin smears for transmission and scanning electron microscopy levels respectively) and for SDS-PAGE electrophoresis and subsequent western

1] Boomsma CM, Eijkemans MJ, Hughes EG, et al. A meta-analysis of pregnancy outcomes in women with polycystic ovary syndrome. Hum Reprod Update 12:673-83,2006. [2] Hayashi M, Nakai A, Satoh S, et al. Adverse obstetric and perinatal outcomes of singleton pregnancies may be related to maternal factors associated with infertility rather than the type of assisted reproductive technology procedure used. Fertil Steril 98:922-928,2012. [3] Chen XK, Wen SW, Bottomley J, et al. In vitro fertilization is associated with an increased risk for preeclampsia. Hypertens Pregnancy 28:1-12,2009. [4] Thomopoulos C, Tsioufis C, Michalopoulou H, et al. Assisted reproductive technology and pregnancy-related hypertensive complications: a systematic review. J Hum Hypertens 27:148-57,2013. [5] Haavaldsen C, Tanbo T, Eskild A. Placental weight in singleton pregnancies with and without assisted reproductive technology: a population study of 536,567 pregnancies. Hum Reprod 27:576-82,2012. [6] Maheshwari A, Pandey S, ...

We developed methods for the cryopreservation of sperm of the endangered Colorado pikeminnow Ptychocheilus lucius. Sperm were collected from a captive broodstock population of Colorado pikeminnow reared and maintained at the Dexter National Fish Hatchery and Technology Center. Our objectives were to (1) evaluate the effects on sperm motility of 24-h storage in Hanks balanced salt solution (HBSS); (2) characterize sperm motility and duration; (3) examine the relationship between sperm motility and osmotic pressure; (4) examine the effect of four cryoprotectants (dimethyl sulfoxide [DMSO], dimethyl acetamide [DMA], glycerol, and methanol [MeOH] at two concentrations [5% and 10%]) on postthaw motility; and (5) compare the effect of two cooling rates (40??C/ min and 4??C/min) on postthaw motility. The sperm samples diluted with HBSS retained higher motility (mean ??SD, 77 ?? 22%; n = 9) than did undiluted samples (12 ?? 30%; n = 9) after 24 h of storage. When exposed...

Contents: Foreword; Preface; Part I. Introduction: 1. The evolution of ART; 2. The effect of chemotherapy and radiotherapy treatment for cancer on the reproductive system in humans; 3. Fertility preservation in non-cancer patients; Part II. Cancer Biology, Epidemiology and Treatment: 4. Basic cancer biology and immunology; 5. Breast cancer and fertility preservation: a view from oncology; 6. Breast cancer therapy and reproduction; 7. Pediatric cancer therapy and fertility; 8. Cancer epidemiology and environmental factors in children, adolescents and young adults; Part III. Reproductive Biology and Cryobiology: 9. Life and death in the germ line: apoptosis and the origins of DNA damage in human spermatozoa; 10. Molecular aspects of follicular development; 11. Fundamental cryobiology of reproductive cells and tissues: concepts and misconceptions; 12. Fundamental aspects of vitrification as a method of reproductive cell, tissue, and organ cryopreservation; Part IV. Fertility Preservation Strategies ...

Efficient Long-Term Cryopreservation of Pluripotent Stem Cells at −80 °C A medium comprised of Ficoll 70 and dimethyl sulfoxide in presence or absence of fetal bovine serum can provide reliable cryopreservation of various kinds of human and porcine pluripotent stem cells at −80 °C for periods that extend up to at least one year, with the post-thaw viability. [Sci Rep] Full Article Umbilical Cord-Derived Mesenchymal Stem Cells Reversed the Suppressive Deficiency of T Regulatory Cells from Peripheral Blood of Patients with Multiple Sclerosis in a Co-Culture - A Preliminary Study Researchers investigated whether umbilical cord-derived mesenchymal stem cells (UC-MSCs) could improve the impaired function of T regulatory cells from multiple sclerosis (MS) patients. Co-cultures of UC-MSCs with PBMCs of MS patients were performed for three days. [Oncotarget] Full Article Human Umbilical Cord Derivatives Regenerate Intervertebral Disc Researchers investigated the feasibility and efficacy of human ...

Lipid peroxidation (LPO) of stallion spermatozoa was assessed in fresh semen and in samples of the same ejaculates after freezing and thawing. Particular attention was paid to individual differences in the susceptibility to LPO and its possible relationship with freezability. Innate levels of LPO were very low in fresh spermatozoa but increased after thawing, a change that was largely stallion-dependent. The level of LPO in fresh spermatozoa was not correlated with that of the thawed spermatozoa. Negative correlations existed between LPO and intact membranes post-thaw (r= -0.789, Pless than0.001), and also between LPO and spermatozoa with high mitochondrial membrane potential (Delta psi m) post-thaw (r= -0.689, Pless than0.001). LPO was also highly and significantly correlated with caspase activity. The correlation between caspase activity in ethidium positive cells and LPO was r=0.772, Pless than0.001. This LPO is unlikely to represent, per se, a sign of cryopreservation-induced injury, but it ...

The aim of this study was to evaluate the applicability of the Cryotech technique for the vitrification of domestic cat (Felis catus) oocytes, as a model for other feline species threatened with extinction. This technique, in which oocytes are stored in a minimal volume of medium, is already widely used in human assisted reproductive technology. In the first part of this study, a viability test (EtBr/FDA) was used to evaluate the toxicity of the vitrification media (solutions). After IVM, oocytes were placed in vitrification and warming solutions according to the manufacturers procedure, with or without exposure to liquid nitrogen. The solutions and the vitrification procedure each caused a reduction in oocyte viability, with survival rates of 71.4% in oocytes exposed to the Cryotech media (without cooling in liquid nitrogen), and 62% in oocytes that were vitrified. In the second part of the experiment, parthenogenetic activation was used to evaluate the developmental potential of oocytes previously

At this moment KrioRus cryopreserves 15 foreign patients. Three are from Ukraine, three are from Italy and there is one from each of the following countries: Australia, France, Belarus, Estonia, Japan, Israel, the Netherlands, Switzerland, the United States. People from other countries are also among the clients who sign up a cryonics contract in advance. They include citizens of Belarus, the United Kingdom (2), Denmark and Ukraine (also 3 people).. ...

As part of the Sperm Bank of Hawaii, the Fertility Institute of Hawaii will cryopreserve or freeze sperm for the partner or spouse of the sperm donor to use. Unlike the womans eggs, sperm is much less affected by the aging process. Therefore, men rarely need to freeze sperm secondary to advanced age.. ...

Introduction: Cryopreservation is possible for all stages of pre-implantation embryos. It has been reported that survival rate of blastocyst is comparably lower than other stages. There is a high volume of fluid in blastocoel cavity that can be a good ground for ice crystals formation, resulting in damage to the cell structure. In this study, the effects of artificial collapseand reduction of the fluid volume in blastocyst cavity before vitrification process on the survival rate and quality of blastocysts were assessed by estimating the expression levels of Oct4, Nanog, Sox2, and Klf4.. Materials & Methods: Mouse blastocysts divided into 5 groups including: A) Vitrified- thawed blastocysts, B) Vitrified- thawed blastocysts after artificial collapse, C) Collapsed blastocysts, D) Immersed blastocysts in vitrification/warming solutions and E) Fresh blastocysts as control group. The survival and hatching rate of embryos were evaluated and the expression of pluripotency-specific genes was assessed by ...

Egg cryopreservation, preserving eggs by cooling to sub-zero temperatures, includes freezing eggs which first took place in 1986. Since then, over 500 children worldwide have been conceived from frozen eggs.. Vitrification is an alternative to egg freezing, first applied in humans in 1999. The process involves cooling cells to form an ice-free transparent glass. Since this process was first used, there have been well over 450 births reported.. The review adds that vitrification can be simpler, more efficient and cheaper than conventional freezing under certain circumstances. It is quicker than freezing for a small group of eggs collected from a single donor, whereas freezing may be more efficient when eggs are to be stored for several women on the same day. Expensive cooling apparatus is not required but vitrification consumables are currently expensive and embryologists may have to devote more time to vitrification than freezing.. As the application of vitrification for human egg storage is ...

Before freezing, embryos are first equilibrated in special solutions containing cryoprotecting agents. These agents protect the embryos from intracellular ice formation, which would be detrimental to their viability. The embryos are then placed in special straws, sealed and put in a machine with an integrated computer. This machine, the programmable freezer, lowers the temperature in a slow controlled manner until -196oC. There are several protocols of slow freezing, depending on the stage of the embryos and the type of cryoprotectant solutions used. The embryos are then plunged in liquid nitrogen and are stored until used.. Usually 1-3 embryos are placed in each straw. In this state, embryos may be preserved for a very long period of time. Embryos can be frozen at the 2PN stage (Day 1), cleavage stage (2-8 cells; Days 2-3), or blastocyst stage (Days 5-6 post oocyte retrieval).. Cryopreservation in liquid nitrogen at a temperature of -196oC does not require electric power. The only requirement ...

Volume 2 Number 9 - December 1997. Prof. Valentina L. Tikhonova and Dr Igor A. Smirnov. A comparative study on the influence of storage at -20ºC and cryopreservation (-196ºC) for one month on the laboratory germinability of seed for more than 150 species was carried out. The germination, growth and development of plants in field conditions from seed after storage at different temperatures have been studied for 30 species. The influence of non-deep and deep freezing of seed on the growth and development of succeeding generations of plants (10 species) was investigated. Very interesting data were acquired on the prolongation of life of microbiotic seeds, on the long-term preservation of the genetic resources of 25 species of medicinal plants and on the influence of cryopreservation on seeds of various families. The experimental work showed considerable potential for the cryopreservation of seed of native plants.. A database of the anatomy, morphology and the biology of seeds of native species of ...

Ehren turned to a local fertility group, Reproductive Partners-UCSD Regional Fertility Center, for help. Like a growing number of other young cancer patients, she decided on fertility preservation to maximize her options for the future. Fertility preservation -freezing a patients eggs or embryos before undergoing potentially damaging cancer treatments-gives women the opportunity to pursue In Vitro Fertilization (IVF) years later when they are healthy and cancer-free. ...

Hepatocytes are widely used in the pharmaceutical and medical fields for drug metabolism studies, bioartificial liver devices, and repopulation of damaged livers as an alternative to transplantation. However, these cells are scarce and difficult to maintain in culture for prolonged periods of time. Banks of cryopreserved liver cells would significantly alleviate issues of hepatocyte availability, and efforts are being made to improve the viability and functionality of frozen hepatocytes. Previously, most work on improving post-thaw viability has hinged on limiting the physical damage of freezing by adding cryoprotective agents and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by freeze-tolerant animals have also been used. A non-metabolizable glucose analog, 3-0-methyl- glucose (30MG), has shown promise with hepatocytes and was used in ...

Cryobiology is the branch of biology that studies the effects of low temperatures on living things within Earths cryosphere or in science. The word cryobiology is derived from the Greek words κρῧος [kryos], cold, βίος [bios], life, and λόγος [logos], word (hence science). In practice, cryobiology is the study of biological material or systems at temperatures below normal. Materials or systems studied may include proteins, cells, tissues, organs, or whole organisms. Temperatures may range from moderately hypothermic conditions to cryogenic temperatures. At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water. This usually requires the ...

In a Frozen Embryo Transfer cycle, the uterine lining may be prepared using hormones or the transfer may be performed using a natural cycle. The embryo(s) is/are thawed and transferred to the uterus in the same process as in a fresh IVF cycle.

In a Frozen Embryo Transfer cycle, the uterine lining may be prepared using hormones or the transfer may be performed using a natural cycle. The embryo(s) is/are thawed and transferred to the uterus in the same process as in a fresh IVF cycle.

Vitrification (from Latin vitreum, glass via French vitrifier) is the transformation of a substance into a glass, that is to say a non-crystalline amorphous solid. In the production of ceramics, vitrification is responsible for its impermeability to water. Vitrification is usually achieved by heating materials until they liquidize, then cooling the liquid, often rapidly, so that it passes through the glass transition to form a vitrified solid. Certain chemical reactions also result in glasses. In a wider sense, the embedding of material in a glassy matrix is also called vitrification. An important application is the vitrification of radioactive waste to obtain a stable compound that is suitable for ultimate disposal. In terms of chemistry, vitrification is characteristic for amorphous materials or disordered systems and occurs when bonding between elementary particles (atoms, molecules, forming blocks) becomes higher than a certain threshold value. Thermal fluctuations break the bonds; ...

Major Finding: Many oncologists are not likely to mention fertility preservation to cancer patients who have a poor prognosis, contrary to guidelines.. Data Source: A national 53-item survey of 513 U.S. oncologists.. Disclosures: The study was sponsored by the American Cancer Society. Dr. Quinn and her associates stated that they have no conflicts of interest.. ATLANTA - Physicians attitudes may conflict with recommended guidelines for fertility preservation and reduce the likelihood that cancer patients will receive information about this reproductive option.. That conclusion was drawn from a national 53-item survey of 513 U.S. oncologists, specifically examining physicians attitudes toward fertility preservation referral among cancer patients with a poor prognosis.. Sixty percent of respondents agreed with the statement that fertility preservation is a high priority for me to discuss with newly diagnosed cancer patients, while 26% were unsure and 14% disagreed, Gwendolyn Quinn, Ph.D., and ...

Vitrification is the safest method to cryopreserve oocytes, however the process alters mitochondrial function resulting from increased reactive oxygen species (ROS) production. Our aim was to alleviate ROS stress in vitrified mice oocytes using N-acetylcysteine (NAC at 1 mM), to improve the oocytes developmental competence. Hence, four experimental groups were compared: fresh oocytes (F-C), vitrified oocytes (V-C), NAC addition prior to oocyte vitrification (V-NAC-Pre) and NAC addition after vitrification (V-NAC-Post). V-NAC-Pre and V-NAC-Post exhibited higher levels of mitochondrial polarization compared to vitrified oocytes (36.5 ± 3.1, 37.7 ± 1.3 and 27.2 ± 2.4 measured as the spatial coefficient of variation/oocyte respectively, mean ± SEM; p | 0.05). However, ROS production increased in vitrified oocytes added with NAC compared to the vitrified control (1124.7 ± 102.1 [V-NAC-Pre] and 1063.2 ± 82.1 [V-NAC-Post] vs. 794.6 ± 164.9 [V-C]; arbitrary fluorescence units/oocyte, mean ± SEM; p | 0

Dustin R. Wakeman, Benjamin M. Hiller, David J. Marmion, Christopher W. McMahon, Grant T. Corbett, Kile P. Mangan, Junyi Ma, Lauren E. Little, Zhong Xie, Tamara Perez-Rosello, Jaime N. Guzman, D. James Surmeier, and Jeffrey H. Kordower. Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell Reports j Vol. 9 j 1-13 j July 11, 2017. http://dx.doi.org/10.1016/j.stemcr.2017.04.033. ...

TY - JOUR. T1 - Cryopreservation of the model alga Ectocarpus (Phaeophyceae). AU - Heesch, Svenja. AU - Day, John. AU - Yamagishi, T. AU - Kawai, H. AU - Küpper, Frithjof. PY - 2012. Y1 - 2012. M3 - Article. SP - 327. EP - 336. JO - Cryoletters. JF - Cryoletters. SN - 0143-2044. ER - ...

EN] The present study was designed to determine whether different calcium concentrations in the vitrification solutions could improve the developmental competence of in vitro matured ovine oocytes after cryopreservation. In vitro matured oocytes were vitrified with 16.5% ethylene glycol (EG) + 16.5% dimethylsulfoxide (DMSO) vitrification media. The base media contain different calcium concentrations, so that five experimental groups were obtained: TCM/FCS (TCM 199 + 20% fetal calf serum (FCS), [Ca2+] 9.9 mg/dl); PBS/FCS (Dulbecco Phosphate Buffered Saline (PBS) + 20% FCS, [Ca2+] 4.4 mg/dl); PBSCaMg (free)/FCS (PBS without Ca2+ and Mg2+ + 20% FCS [Ca2+] 2.2 mg/dl); PBS/BSA (PBS + 0.4% bovine serum albumin (BSA), [Ca2+] 3.2 mg/dl) and PBSCaMg (free)/BSA (PBS without Ca2+ and Mg2+ +0.4% BSA, [Ca2+] 0.4 mg/dl). After warming, the oocytes from the five experimental groups were assessed for survival, spontaneous parthenogenetic activation and developmental capacity via in vitro fertilization. Oocyte ...

OBJECTIVE: To analyze demographic and clinical data of patients who resorted to oocyte freezing between January 2014 and December 2018. STUDY DESIGN: Patients who applied to the Reproductive Endocrinology and Infertility Unit of Ankara University School of Medicine between January 2014 and December 2018 with the request of oocyte freezing were included in this study. The files and computer records of the patients were analyzed retrospectively and sociodemographic, clinical and laboratory data were evaluated. RESULTS: A total of 46 cycles were recorded in 40 patients over a 5-year period. The main indications were low ovarian reserve and/or advanced age (68.3%) and malignancy diagnosis (31.7%). There was a significant difference between elective fertility preservation and oncofertility preservation (Onco-FP) groups in terms of the age (38.4±4.7 vs 28.4±6.1; p=0.001). There was a significant difference between two groups in favor of oncofertility group in terms of anti-Mullerian hormone level, ...

Looking for online definition of cryopreserved in the Medical Dictionary? cryopreserved explanation free. What is cryopreserved? Meaning of cryopreserved medical term. What does cryopreserved mean?

A few weeks prior to the date you anticipate needing the samples, contact the andrology lab so that we can bring the samples out of long-term storage. If you plan to use the samples for artificial insemination, we will need to have a physicians requisition order on file. When you know the day you will need the sample prepared, call and set an appointment to pick the sample up.. Many physicians have additional requirements, such as blood tests or a current pap and pelvic exam, that they require before they will perform an insemination. Be sure to communicate with your physician in advance to avoid any delays.. ...

TIANCHI Small Liquid Nitrogen Tanks 3 Liter LN2 Dewar Container Cryo Tank. Net Weight(KG):3.2. Gross Weight(KG):5.0. Static Power Dissipation(Kg/d):0.08. This Small Liquid Nitrogen Tanks 3 Liter is made of high-strength, special corrosion resistant aluminum alloy, light weight and easy to carry. Liquid nitrogen storage containers is well made, very good insulation, integrated molding, weld the small, solid and reliable, guarantee vacuum and prevent leakage. Liquid nitrogen container low loss, more than 5 years of vacuum quality.. Liquid nitrogen storage tank can be stored for a long biological specimens, semen, embryos, spawn, vaccines, skin, organ and other biological material. Products three guarantees service, for each customer to establish user files, and enjoy lifelong technical services. This LN2 Dewar Container is only suitable for the static storage of liquid nitrogen, not suitable for the transport of liquid nitrogen. This cryogenic container ships empty. It can be filled anywhere that ...

Hai manteman blogger tercinta dan terkasih, lama tak bersua yaa ... Kali ini aku mau cerita seputar kehamilan kedua aka adeknya Den Gus Ndornying yang sekarang udah di minggu ke 21. Kehamilan kali ini emang direncanakan seperti Miká waktu itu, tapi karena kami punya simpanan embrio di freezer jadi istilahnya Frozen Embryo Transfer. Sebelum proses…

These hormone trransfer that make you emotional during your month-to-month cycle stick round for early being pregnant, too. However some ladies we frozen embryo transfer and ectopic pregnancy to felt bodily high quality through the first few weeks, or observed solely minor adjustments. When youve been charting your basal physique temperature to determine if you ovulate, its continued elevation for more than two weeks may imply that you justre pregnant. I made a list of some of the embryp that helped me most in getting ready for my natural delivery, and I hope you will find them useful as nicely. This proof, along with the data from the current examine, gives health care providers, notably ob-gyn practitioners, the knowledge wanted to counsel girls about the hazards of smoking and secondhand smoke, and to encourage cessation, Folan mentioned. An image of a fetus at week 23, with a extra pronounced listening to skill and awareness of the mothers each transfer. Material on this web site is meant ...

A new study has found that adolescents and young adults undergoing cancer treatment gain coping skills and resilience-related outcomes when they participate in a therapeutic music video (TMV) process.