TY - JOUR. T1 - Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4. AU - Komoto, Satoshi. AU - Kugita, Masanori. AU - Sasaki, Jun. AU - Taniguchi, Koki. PY - 2008/7. Y1 - 2008/7. N2 - Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.. AB - Recombinant rotavirus (RV) with ...
At the American Association for the Advancement of Science meeting in Boston last weekend, Emory Vaccine Center researcher Mark Mulligan presented some limited findings on immune responses in Zika-infected humans, who were returned US travelers or expatriates.. The results were intriguing, despite the small number of study participants: five, two of whom were pregnant. Detailed information has not been available about immune responses against Zika in humans, especially T cell responses.. Highlights from Mulligans abstract:. *All five seemed to have a hole in their immune systems - functional antiviral killer CD8 T cells were rare, despite activation of CD8 T cells in general and strong responses from other cell types.. *Cross-reactive immune responses, based on previous exposure to dengue and/or yellow fever vaccine, may have blunted Zikas peak.. *Even with prolonged maternal viremia, both pregnancies resulted in live births of apparently healthy babies.Zika infection during pregnancy can ...
Newcombe, J, Eales-Reynolds, L, Wootton, L, Gorringe, AR, Funnell, SGP, Taylor, SC and McFadden, JJ (2004) Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity ...
Antigen cross-reactivity is an inbuilt feature of the T cell compartment. However, little is known about the flexibility of T cell recognition in the context of genetically variable pathogens such as HIV-1. In this study, we used a combinatorial library containing 24 billion octamer peptides to characterize the cross-reactivity profiles of CD8+ T cells specific for the immunodominant HIV-1 subtype B Nef epitope VY8 (VPLRPMTY) presented by HLA-B*35∶01. In conjunction, we examined naturally occurring antigenic variations within the VY8 epitope. Sequence analysis of plasma viral RNA isolated from 336 HIV-1-infected individuals revealed variability at position (P) 3 and P8 of VY8; Phe at P8, but not Val at P3, was identified as an HLA-B*35∶01-associated polymorphism. VY8-specific T cells generated from several different HIV-1-infected patients showed unique and clonotype-dependent cross-reactivity footprints. Nonetheless, all T cells recognized both the index Leu and mutant Val at P3 equally ...
Three anti-horse liver alcohol dehydrogenase (HLADH) monoclonal antibodies are described. Two are specific for ADH and cross-react with class I and II enzymes from mouse, horse and Chinese hamster....
TY - JOUR. T1 - Formalin-inactivated EV71 vaccine candidate induced cross-neutralizing antibody against subgenotypes B1, B4, B5 and C4A in adult volunteers. AU - Chou, Ai Hsiang. AU - Liu, Chia Chyi. AU - Chang, Jui Yuan. AU - Jiang, Renee. AU - Hsieh, Yi Chin. AU - Tsao, Amanda. AU - Wu, Chien Long. AU - Huang, Ju Lan. AU - Fung, Chang Phone. AU - Hsieh, Szu Min. AU - Wang, Ya Fang. AU - Wang, Jen Ren. AU - Hu, Mei Hua. AU - Chiang, Jen Ron. AU - Su, Ih Jen. AU - Chong, Pele Choi Sing. PY - 2013/11/21. Y1 - 2013/11/21. N2 - Background: Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia. No effective EV71 vaccine is available. A randomized and open-label phase I clinical study registered with ClinicalTrials.gov #NCT01268787, aims to evaluate the safety, reactogenicity and immunogenicity of a formalin-inactivated EV71 vaccine candidate (EV71vac) at 5- and 10-μg doses. In this study we report the cross-neutralizing antibody responses from each ...
In a subset of patients with limited cutaneous (lc) systemic sclerosis (SSc), anti-CENP-A antibodies (Ab) cross-react with a peptide (FOXE3p53-62) that presents striking homology with one of the two immunodominant epitopes of CENP-A (Ap17-30). We searched for clinical correlates of anti-FOXE3p53-62 Ab by measuring their levels along with those of Ab to Ap17-30 and to the second immunodominant epitope of CENP-A, namely Ap1-17. Serum samples were obtained from 121 patients with SSc, 46 patients with systemic lupus erythematosus (SLE) and 25 healthy blood donors (HBD). The reactivity of serum IgG to Ap1-17, Ap17-30 and FOXE3p53-62 was measured by ELISA. The corresponding anti-peptide Ab were affinity-purified from pooled SSc sera and used to establish standard curves for quantifying these Ab in patients and HBD. Receiver operating characteristics (ROC) analysis, comparing SSc patients who were positive for anti-CENP Ab (ACA+) to those who were negative, was used to find cut-off points for dichotomizing the
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This thesis describes the investigation of the characteristics of cultured MSC with a special focus on the MSCs with in vivo bone forming capacity. The most relevant contributions of the work described in this thesis to the field of regenerative medicine, and bone tissue engineering research field in particular, are ... read more the following: 1) The identification of novel cross-reacting monoclonal antibodies for prospective isolation of primary MSCs (with clonogenic potential) from multiple mammalian species that are widely used in regenerative medicine research; 2)We investigated the use of human platelet lysate (PL) for MSC expansion as a substitute for fetal bovine serum (FBS). Use of PL enables MSCs expansion under GMP conditions in a relatively short time period, retaining a common phenotype and multipotency. This procedure is relevant for clinical applications that require high numbers of MSCs, such as in the treatment for graft-versus-host disease, Crohns disease, and rheumatoid ...
Abbkine Scientific has announced the launch of its new kit, the EliKine™ Human IL-2 ELISA Kit otherwise known as Human IL2 ELISA Kit. The launch of the product reiterates the companys commitment to enhancing research and investigation in the field of life science.. The product has a human reactivity, employing a two-site sandwich ELISA to quantitate IL-2 in samples. With colorimetric detection method and assay duration with multiple steps standard sandwich ELISA assay with a working time of 3-5 hours, the kit stands tall amongst its peers on the market.. The featured kit includes Human IL-2 microplate, Human IL-2 standard, Human IL-2 detect antibody, EliKine™ Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer and Plate covers.. The major feature and benefit of the kit besides having a calibration range of 3.9 pg/ml-250 pg/ml is its high sensitivity and excellent specificity for detection of Human IL-2. It also has no significant cross-reactivity or ...
Human TNF-α ELISA Kit is the latest product from Abbkine Scientific Research Company. As part of the companys plans to ease the research process and ensure that better results are gotten in record time, the company made the announcement to official launch the Human TNF alpha ELISA Kit on the market.. The ELISA Kit is particularly designed to allow for the easy detection of Human TNF-α. Consequently, investigators, researchers and other such users of the product can easily get their desired results in record time. This also ensures that better results are gotten.. EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples, it has high sensitivity and excellent specificity for detection of Human TNF-α. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.. The recent launch of the Human TNF-α ELISA Kit has been described by many as a revolutionary introduction to the industry. With a high sensitivity and ...
Semantic Scholar extracted view of Cross-Reactive Peptides in Memory T Cell Memory CTL: Potential Role of Peptide Antigens Is Sufficient to Activate A Single Specific Amino Acid Residue in by Riccardo Gavioli et al.
Objectives. This study tested the hypothesis, first proposed by Chaussinand, that individual-level immunity acquired from exposure to tuberculosis may have contributed to the disappearance of leprosy from western Europe. Methods. The epidemiological consequences of cross-immunity were assessed by the formulation of a mathematical model of the...
|strong|Mouse anti Human hCG anibody, clone INN-hCG-72|/strong| recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in d…
Swine flu has been in the headlines for days now. The headlines change every day. By the time this article is printed, the situation will have changed even more. Therefore, it is hard to write a timely article on this particular disease. There are some facts that bear no relation to time. The first is that we have flu epidemics every year. They usually occur in the December to February time frame. We start an immunization program for it prior to that. The viruses contained in the immunization are scientists best guesses as to which flu viruses are going to be prevalent that particular year. They usually guess correctly so many people are protected before each years outbreak. One of the issues with swine flu is that there is currently no immunization for it. Cross reaction to other flu strains is not present. Therefore, no one is immune to the virus. For that reason many more people would be affected by an outbreak. The second fact is that every year people who get the flu die from its ...
Centralized (ancestral and consensus) HIV-1 envelope immunogens induce broadly cross-reactive T cell responses in laboratory animals; however, their potential to elicit cross-reactive neutralizing antibodies has not been fully explored. Here, we report the construction of a panel of consensus subtype B (ConB) envelopes and compare their biologic, antigenic, and immunogenic properties to those of two wild-type Env controls from individuals with early and acute HIV-1 infection. Glycoprotein expressed from full-length (gp160), uncleaved (gp160-UNC), truncated (gp145), and N-linked glycosylation site deleted (gp160-201N/S) versions of the ConB env gene were packaged into virions and, except for the fusion defective gp160-UNC, mediated infection via the CCR5 co-receptor. Pseudovirions containing ConB Envs were sensitive to neutralization by patient plasma and monoclonal antibodies, indicating the preservation of neutralizing epitopes found in contemporary subtype B viruses. When used as DNA vaccines ...
An agglutination test used in the laboratory to diagnose rickettsial diseases. It depends on a nonspecific cross reaction between antibodies produced by the rickettsial infection with the OX-2, OX-19 and OXK antigens of the Gram negative rod, Proteus.. ...
The participants, recruited by advertisements in two daily newspapers, were obese and asthmatic. Inclusion criteria were ability to cope with the study protocol, body mass index 30-42, age 18-60 years, previously diagnosed asthma with a spontaneous diurnal variation or a bronchodilator response of 15% or more, and being a non-smoker or having stopped smoking for two years or more before age 50. Exclusion criteria were pregnancy, history of bulimia or anorexia, unstable angina or arrhythmia, untreated thyroid disease, symptomatic liver or gall bladder disorder, any other severe disease, insulin treatment, systemic steroid treatment, or history of food allergy or of intolerance to any component of the very low energy dietary preparation that would be used in the study-such as soya, fish, chocolate, or lactose. Participants with a history of adverse reactions to peas, beans, or peanuts were excluded because of possible cross reactions to soya protein.3. Telephone interviews were held with 202 ...
The participants, recruited by advertisements in two daily newspapers, were obese and asthmatic. Inclusion criteria were ability to cope with the study protocol, body mass index 30-42, age 18-60 years, previously diagnosed asthma with a spontaneous diurnal variation or a bronchodilator response of 15% or more, and being a non-smoker or having stopped smoking for two years or more before age 50. Exclusion criteria were pregnancy, history of bulimia or anorexia, unstable angina or arrhythmia, untreated thyroid disease, symptomatic liver or gall bladder disorder, any other severe disease, insulin treatment, systemic steroid treatment, or history of food allergy or of intolerance to any component of the very low energy dietary preparation that would be used in the study-such as soya, fish, chocolate, or lactose. Participants with a history of adverse reactions to peas, beans, or peanuts were excluded because of possible cross reactions to soya protein.3. Telephone interviews were held with 202 ...
Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Killian on positive skin tb test: There is no cross reaction between them. By the way, up to 5% of normal individuals may test positive to ANA test, usually at low titer. for topic: Positive Skin Tb Test
I am a bioengineer fascinated by T cell immunology, and I have expertise in single-molecule mechanical analysis, single-molecule imaging, quantum dot nanotechno...
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
A new paper has just appeared in PLoS Medicine on an old topic: whether seasonal influenza vaccines might also cause enough cross-reactivity to protect against H5N1. The basic idea is simple. The immune system sees the surface proteins on the flu virus and makes protective antibodies against them. The major stimulant for this is the hemagglutinin protein (HA), the H part of H5N1. There are 16 different immunological flavors of HA that cross-react very little.
Hi, I have a mouse anti-bovine glutamate dehydrogenase mAb and Im curious whether anyone would know whether it will crossreact with Human GDH. I think the two proteins are fairly similiar but how similiar are humans with cows??? Please send a reply to my E-mail dsho at med.unc.edu Thanx for any thoughts. Moo Moo, Dave Shock ...
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant n, mRNA. (H00004179-R17) - Products - Abnova
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant h, mRNA. (H00004179-R27) - Products - Abnova
We would like to thank Dr. Sylvain Lesne for his interest in our work and for his comments. We would like to clarify some of the points he raised concerning our report, since we disagree with some of his statements.. Regarding Aβ detection, we used the 6E10 antibody to monitor the level of APP transgene expression at the age we performed our analysis of spine density in vivo (i.e., three months postnatal), but our intention was not to characterize the presence of Aβ1-42 oligomers at this age in this mouse strain. They have been extensively characterized previously by others (see below). The 6E10 antibody is known to recognize human APP, CTF-β, and Aβ. We acknowledge that the band running below 15 kDa should be considered as a mixture of CTF/Aβ since tissues were homogenized in RIPA buffer. Regarding the APP signal at ~100 kDa, we could clearly detect a faint signal in the wild-type mice (after overexposure of the membrane), suggesting that the antibody can cross-react with mouse APP. This ...
FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Mouse Monoclonal Antibody Species : Tag : Expression System: Sequence : Specificity :Recognizes the p21CIP1 (p21/WAF1) protein Cited Applications : WB, ELISA, IP Cross Reactivity :Human Host / Isotype / Clone# :Mouse, IgG Immunogen :Produced by repeated immunizatio
FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Rabbit Polyclonal Antibody Species : Tag : Expression System: Sequence : Specificity :Recognizes the Tyrosine Hydroxylase (TH) protein Cited Applications :WB, IF, IHC Cross Reactivity :All mammals Host / Isotype / Clone# :Rabbit, IgG Immunogen :SDS-denatured rat ty
Browse Item # 73-364, LRRTM2 in the Antibodies, Inc. catalog including Item #,Item Name,Description,Target,Applications,Clone,IgG Isotype,Species Reactivity,Validation,Type,Format,Cross Reactivity,Expected Banding Pattern,Host,Label,Antibody Type,Com
|strong|Goat anti Dog IgG1 polyclonal antibody|/strong| specifically recognises canine IgG1 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, …
Browse Item # 73-136, GABA(A)R, Alpha1 in the Antibodies, Inc. catalog including Item #,Item Name,Description,Target,Applications,Clone,IgG Isotype,Species Reactivity,Validation,Type,Format,Cross Reactivity,Expected Banding Pattern,Host,Label,Antibod
Anne Schuchat: I think one possibility is that older people might have been exposed to H1N1 strains a long time ago that might have some relation to the strain were seeing now. Our virologists have compared it to all these other strains and this is really different. This is not a close, genetic relative of any of the H1N1s that have been a problem in people, but the immunologic characteristics might be different than the genetic characteristics and so it would be very great if older people had been exposed to some H1N1 a long time ago that had some relationship with this one immunologically and that might lead to that pre-existing immune they we hope is there, but were not sure is there. So we dont have a particular virus that were thinking about because the genetic characteristics are so different, but we are wondering about whether there were some viruses around back in the 30s, 40s or 50s that might be immunologically similar to the one were seeing now ...
anti-Ad5 Hexon, mAb (65H6) is a monoclonal antibody that crossreacts with Virus protein. Works in WB, ICC. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
Preamble: Stanford points out that the average 70-year-old is on 7 (seven) pharmaceutical agents1. Since literally hundreds of potential adverse reactions can occur with each medication, imagine the potential for cross reactivity (same reference quotes an average number of potential side effects per drug to be 69)…especially if a drugs metabolite can impart adverse reactions too?
anti-STAT2, pAb is a polyclonal antibody that crossreacts with human protein. Works in WB. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
, ATF2 blocking peptide, GTX89979-PEP, Applications: Apuri, Blocking, ELISA; Affinity purification, Blocking, ELISA; CrossReactivity: Human|Dog|Rat
, M-CSF recombinant protein, GTX65335, Applications: Apuri, Blocking, ELISA, Functional Assay; Affinity purification, Blocking, ELISA, Functional Assay; CrossReactivity: Mouse
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Since August 2011, a total of 12 human infections with influenza A (H3N2) variant viruses with genes from avian, swine, and human viruses (i.e., A [H3N2]v) that had acquired the M gene from influenza A (H1N1)pdm09 virus have been reported to CDC. Eleven of the cases occurred in children aged ,10 years. In six cases, no history of recent exposure to swine was noted, suggesting that human-to-human transmission had occurred (1-3). This new gene constellation for A (H3N2)v viruses and its temporal association with an increase in human cases of A (H3N2)v highlight the need to better understand the risk for human infection with these viruses and the extent to which current seasonal vaccines might elicit cross-reactive antibodies to them. CDC conducted a preliminary analysis to evaluate the age-specific presence of serum cross-reactive antibody in U.S. populations vaccinated or not vaccinated with the 2010-11 seasonal trivalent influenza vaccine (TIV). The results indicated that 1) little or no ...
Control of swine influenza by vaccination has become more difficult in recent decades, as the evolution of the virus has resulted in inconsistent responses to traditional vaccines. Standard commercial swine flu vaccines are effective in controlling the infection when the virus strains match enough to have significant cross-protection, and custom (autogenous) vaccines made from the specific viruses isolated are created and used in the more difficult cases.[80][81] Present vaccination strategies for SIV control and prevention in swine farms typically include the use of one of several bivalent SIV vaccines commercially available in the United States. Of the 97 recent H3N2 isolates examined, only 41 isolates had strong serologic cross-reactions with antiserum to three commercial SIV vaccines. Since the protective ability of influenza vaccines depends primarily on the closeness of the match between the vaccine virus and the epidemic virus, the presence of nonreactive H3N2 SIV variants suggests that ...
Extensive cross-reactivity between the different individual species of the genus could be expected, but has not been formally evaluated. (8). A class I chitinase has been reported to be the relevant protein linked to cross-reactions in latex-fruit allergy syndrome, which includes passion fruit, cherimoya, kiwi, papaya, mango, tomato and wheat. (5, 9). A class I chitinase from avocado or latex extract was used as an inhibitor in a study the aim of which was to evaluate the role of chitinases and complex glycans as cross-reactive determinants linked to latex-food allergy. Putative class I chitinases of 30 to 45 kDa were recognised by both specific polyclonal antibodies to chitinases and sera from patients with latex-fruit allergy in chestnut, cherimoya, passion fruit, kiwi, papaya, mango, tomato, and wheat flour extracts. Prs a 1, the major allergen and class I chitinase from avocado, along with the latex extract, strongly or fully inhibited IgE binding by these components when tested in ...
Blair, P B.; Weiss, D W.; and Smith, G H., Studies on antigenic cross-reactivity of oncogenic rna viruses. Cross-reactions between mouse mammary tumor viruses from different mouse strains. (1970). Subject Strain Bibliography 1970. 940 ...
We determined the antigenic specificity and protective immunogenicity of two chemically synthesized peptides of type 5 streptococcal M protein. The synthetic peptides, designated S-M5(1-20) and S-M5(20-40), represent the amino-terminal amino acid sequence of the native pepsin-extracted M5 molecule, which is known to contain at least one heart cross-reactive epitope. Initial studies showed that neither of the synthetic peptides was able to bind purified heart-reactive M5 antibodies. In addition, S-M5(1-20), but not S-M5(20-40), contained type-specific antigenic determinants as measured by enzyme-linked immunosorbent inhibition assays. When covalently linked to tetanus toxoid, S-M5(1-20), but not S-M5(20-40), evoked significant levels of type-specific, opsonic (and presumably protective) antibodies in rabbits without evoking heart cross-reactive antibodies. ...
Sandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a conventional sandwich ELISA protocol uses a carbohydrate-binding module (CBM), a small protein domain found in some carbohydrate catalysing or activating enzymes, and rat monoclonal antibodies (mAbs) which can be combined in the same ELISA plate without risk of cross reaction; the secondary anti-rat HRP antibody being only able to bind to the rat mAb and not the CBM. This protocol was developed and modified in the Prof. J. Paul Knox lab at the University of Leeds.
In order to establish criteria for the Serodiagnosis of foamy virus infections we investigated the extent to which sera from iofected individuals of human and primate origin react with structural and non-structural virus proteins in immunoblot assays. Using lysates from infected cells as the source of virus antigen, antibodies were preferentially detected against the Gag proteins and the non-structural Bet protein. Both the Gag precursor molecules of 70 and 74K apparent M\(_r\) and the cytoplasmic 60K M\(_r\) Bet protein were found to be phosphorylated, the latter being synthesized in large amounts in infected cells. Rahbit antiserum raised against recombinant human foamy virus (HFV) Gag major capsid protein cross-reacted with foamy viruses of chimpanzee, gorilla, orang-utan, rhesus monkey and Mrican green monkey origin. This was reßected by a broad cross-reactivity of the respective monkey sera to the Gag proteins of the various foamy virus isolates. Cross-reactivity of antisera against the ...
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform ...
OBJECTIVE: The HLA-A30-B13-C06 haplotype is reported to be associated with slow disease progression in the HIV-1-infected Northern Han Chinese population, but the mechanism remains unknown. DESIGN: Gag-specific T-cell responses and gag sequencing were performed in nine B clade HIV-1-infected HLA-A30-B13-C06-positive slow progressors to understand HLA-associated viral control. METHODS: Interferon-γ ELISPOT assays were performed to determine the Gag-specific T-cell responses and cross-reactivity to variant peptides. Longitudinal HIV-1 gag sequencing was performed at the clonal level. RESULTS: The overlapping peptides (OLP)-48: RQANFLGKIWPSHKGRPGNF (RL42 Gag434-453); OLP-2: GQLDRWEKIRLRPGGKKKYR (RL42 Gag11-30); OLP-15: VQNLQGQMVHQPISPRTLNA (RL42 Gag135-154) and OLP-16: HQPISPRTLNAWVKVVEEKA (RL42 Gag144-163) were dominant in HLA-A30-B13-C06-positive patients. A new epitope [HQPISPRTL (Gag144-152, HL9)] within OLP-15 and OLP-16 was identified. Results showed that strong cross-reactive responses to multiple
The experiments recorded above show that in the case of antipneumococcus horse serum or purified antibody the arbitrary immunological procedure (37° for 2 hours, overnight in the ice box) does not permit either the establishment of a true equilibrium or the precipitation of the maximum amount of antibody nitrogen. Analyses of such horse sera for antibody content should therefore be carried out at 0° and the determinations should be allowed to stand in the cold for at least 24 hours in order to insure the completion of the reaction.. It is believed that the similarity of the nitrogen: S III ratios in the specific precipitate, whether obtained from whole serum or from purified antibody, and the failure of added serum to influence the amount of nitrogen precipitated show that the absolute chemical method for the estimation of antibody actually measures antibody and not antibody plus a more or less indefinite amount of non-specific protein. An objection to the use of the method is thus shown to be ...
WÜNSCHMANN, S.; UCHIDA, M.; LEHRER, S. B.; ARRUDA, Luisa Karla de Paula; CHAPMAN, M. D. Purification of native shrimp tropomyosin by affinity chromatography with cross-reactive antibody. Journal of Allergy and Clinical Immunology[S.l: s.n.], 2010 ...
Nisonoff, A and Ju, S, Studies of a cross-reactive idiotype associated with anti-p- -azophenylarsonate antibodies of a/j mice. (1976). Subject Strain Bibliography 1976. 3988 ...
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in BL21. EV71 contamination had been 90.1, 98.4, 98.8 and 88.7%, respectively; likewise, those of IgG antibodies in serum examples had been 82.4, 89.1, 91.5 and 78.1%, respectively. Five of 80 examples (6.25%) from CA16-infected sufferers were detected positive by ELISA with recombinant VP1 proteins where indicated the cross reactions and 0 of 5 examples from sufferers infected with other enteroviruses including CA4, CA5, CB3, CB5 and echovirus 6. As a result, the recombinant VP1 proteins of EV7l might provide a theoretical guide for building a highly effective antibody testing of IgM for EV71-contaminated patients with medically suspected hand, feet, and mouth area disease (HFMD). that may cause severe problems, from minor HFMD to serious neurological syndromes, such as for example encephalitis, pulmonary edema, and death even. Outbreaks of EV71 infections have already been reported ...
So often in veterinary medicine, as in other medical fields, we are looking for diagnostic tests to aid in treatment and to prognosticate for various diseases. Both infectious and non-infectious diseases may be diagnosed by detecting the causative agent, clinical signs, pathologic changes, biochemical changes and/or surrogate evidence of past or present exposure to an agent (antibody). A large number of the tests that we use are able to detect antibody, however, our dilemma is whether that is what is currently producing the clinical signs or is it a past infection. Alternatively, is the reaction we see a cross reaction to some other agent. Seldom do we think about the test in terms of what information it is going to provide basing our recommendations on the prevalence of the disease in your particular practice area. This is commonly referred to in epidemiology circles as the pre-test probability of the disease in question. Prevalence of disease is different for every area, and will differ in the ...
Whether tumors of different lineages share common Ags is a critical issue for understanding anti-tumor immune responses and for designing Ag-specific tumor immunotherapy. Because of lack of cross-protection among individually derived tumors, it has been proposed that tumor Ags are specific for individual tumors. Here we show that lack of cross-protection is not due to lack of a shared tumor Ag. Thus, a plasmocytoma J558 transfected with the costimulatory molecule B7 activates a cross-reactive CTL response in vivo. The major Ag recognized by the cross-reactive CTL is P1A, which is expressed in mastocytoma P815, plasmocytoma J558, and fibrosarcoma Meth A. Surprisingly, no significant cross-protection can be detected among P1A-expressing tumors after immunization with either P1A-expressing or B7-transfected P815 cells. Our results demonstrate that multiple lineages of tumors are not cross-protected even though they share a tumor Ag that can be recognized by CTL. These results have important ...
Two major unanswered questions concerning the specificity of antibodies are: how do structurally different antigens bind with high affinity to the same antibody, and what are the limits of the antibody combining site complementarity and flexibility that contribute to such crossreactivity? We report here a comparative analysis of the X-ray structures of five conformationally different steroids in complex with the Fab fragment of an anti-progesterone antibody DB3 at 2.7 A. This antibody is unable to complement completely the shape of the hydrophobic antigen so that crossreactivity occurs with other ligands without major structural rearrangements of the binding site. Antigen specificity can be explained through conserved interactions of DB3 with the steroid D-ring, whereas some of the crossreactivity is realized through different binding orientations of the steroid skeleton that place the A-ring into alternative pockets on the antibody surface. The restricted gene usage of the VGAM3.8 family in ...
SGS, a leading bio/pharmaceutical, analytical and bioanalytical contract solutions provider, has announced that it has invested in multiple highly sensitive and selective LC-MS/MS instruments at its Poitiers, France, laboratory, for the quantification of proteins and peptide therapeutics.. The two systems, which are now installed and validated, are a combination of the AB SCIEX 6500+ and Shimadzu X2 UPLC, which offers high specificity, a wide dynamic range, faster method development and the ability to quantify multiple proteins simultaneously.. LC-MS/MS techniques have a number of advantages compared with standard conventions for protein and peptide quantitation, which are based on ligand-binding assays (LBAs) such as the enzyme-linked immunosorbent assay (ELISA).. Assay development can often be time-consuming and expensive and, in addition, LBA results are often plagued by interferences and high background from antibody cross-reactivity.. Mass spectrometry platforms have universal ...
Cross-reactive cells are oligoclonal T memory cell expansions. (A) Cross-reactive CD8+ T cells display a restricted BV repertoire. Naive (top) or A/NT/60/68-pri
The exact cause of Guillain-Barré syndrome is unknown. It tends to appear after an infection and as a result of an immune response against nerve tissue. It appears that the immune system forms antibodies to target the antigens of the invading pathogen. These antibodies cross-react with nerve tissue and then directs the immune system to target this tissue. A number of different infections can trigger this immune-mediated response but infections are not the cause of the Guillain-Barré syndrome.. The immune system attacks the outer covering of the nerves known as the myelin sheath. This sheath is responsible for insulating the nerves. When this sheath is damaged it cannot transmit impulses as normal. As a result there is weakening, numbness or paralysis depending on which sensory (sensation) or motor (muscle) nerves are affected. However, in most cases this is not permanent and there is usually complete recovery. In a minority of cases, the weakening, numbness and paralysis may persist.. ...
TY - JOUR. T1 - Inflammatory co-morbidities in HIV+ individuals: learning lessons from healthy ageing. AU - Hearps, Anna Clare. AU - Martin, Genevieve. AU - Rajasuriar, Reena. AU - Crowe, Suzanne Mary. PY - 2014. Y1 - 2014. N2 - Increased life expectancy due to improved efficacy of cART has uncovered an increased risk of age-related morbidities in HIV+ individuals and catalyzed significant research into mechanisms driving these diseases. HIV infection increases the risk of non-communicable diseases common in the aged, including cardiovascular disease, neurocognitive decline, non-AIDS malignancies, osteoporosis, and frailty. These observations suggest that HIV accelerates immunological ageing, and there are many immunological similarities with the aged, including shortened telomeres, accumulation of senescent T cells and altered monocyte phenotype/function. However, the most critical similarity between HIV+ individuals and the elderly, which most likely underpins the heightened risk of ...
Antibody against tubulin from porcine brain was used to evaluate the immunological cross reactivity of tubulin from a variety of animal and plant cells. Indirect immunofluorescence microscopy revealed microtubule-containing structures including cytoplasmic microtubules, spindle microtubules, cilia and fIagella. Thus tubulin from diverse species of both mammals and plants show immunological cross-reactivity with tubulin from porcine brain. Results obtained by immunofluorescence microscopy are whenever possible compared with previously known ultrastructural results obtained by electron microscopy ...
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StabilGuard® Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications. ...
Forgo cross reactivity the selective detection of the ACQUITY UPLC I-Class PLUS/Xevo TQD for methotrexate analysis for clinical research.
By manipulating stem cells, scientists have found they can grow beating cardiac tissue in a petri dish. The cells self-organized to form microchamber ...
Citoxlab has experience with a wide variety of compounds and has a team of pathologists dedicated to running TCR and immunohistochemistry studies.
Selten werden Vorwürfe des Dopings zurückgenommen, im Fall Cris Cyborg Justino geht die UFC-Kämpferin jedoch als Siegerin hervor. Die Sperre von 2016 wird aufgehoben.
Radioimmunoassay (RIA) ist eine Methode, die die Menge eines Antigens in einer Probe mit hoher Empfindlichkeit messen kann. Prinzipiell kann jede biologisc...
Evidence for a Nonspecific Factor Interfering in the Radioimmunoassay of Somatoliberin-Like Immunoreactivity in Human Seminal ...
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