Jeggo, M H. and Wardley, R C., "Generation of cross-reactive cytotoxic t lymphocytes following immunization of mice with various bluetongue virus types." (1982). Subject Strain Bibliography 1982. 3115 ...
In an assay for a target nucleotide sequence employing hybridization with a target probe nucleotide sequence, cross reaction between the target probe and a non-target nucleotide sequence, capable of hybridizing with the target probe, is prevented or diminished by introducing, at some point during the hybridization, a non-target probe which hybridizes preferentially with the non-target nucleotide sequence. The melting temperature for the non-target probe/non-target nucleotide sequence hybrid is preferably greater than that of the target probe/non-target sequence hybrid. The hybridization can be carried out under isothermal conditions or preferentially with gradual cooling.
TY - JOUR. T1 - Immunological cross reaction between calf and Drosophila histones. AU - Bustin, M.. AU - Reeder, R. H.. AU - McKnight, S. L.. PY - 1977. Y1 - 1977. UR - http://www.scopus.com/inward/record.url?scp=0017387756&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0017387756&partnerID=8YFLogxK. M3 - Article. C2 - 856814. AN - SCOPUS:0017387756. VL - 252. SP - 3099. EP - 3101. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 9. ER - ...
Heterosubtypic immunity, defined as cross-reactive immune responses to influenza virus of a different serotype than the virus initially encountered, was investigated in association with virus-specific cytotoxic T lymphocyte (CTL) responses induced in systemic and mucosa-associated lymph nodes after immunization via different routes. Mice immunized by the pulmonary route with live nonpathogenic influenza virus, strain Udorn (H3N2), survived challenge with mouse-adapted pathogenic influenza virus, strain PR/8/34 (H1N1). These mice developed strong heterosubtypic CTL responses in spleen, cervical lymph nodes (CLN), and mediastinal lymph nodes (MLN). Alternately, only 20% of mice immunized intravenously, intraperitoneally, or intranasally survived the challenge; all of these developed CTL responses in spleen and CLN, but not in MLN. Direct correlation between short-term and long-term memory heterosubtypic CTL responses induced in MLN and host recovery after lethal infection indicates that these CTL
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
To assess herd immunity to swine influenza viruses, we determined antibodies in 28 paired serum samples from participants in a prospective serologic cohort study in Hong Kong who had seroconverted to pandemic (H1N1) 2009 virus. Results indicated that infection with pandemic (H1N1) 2009 broadens cross-reactive immunity to other recent subtype H1 swine viruses ...
Effect Diluent™ for dilution of bio samples, to avoid Hook Effects, reduce matrix effects and ensure that the signal response is within the standard curve.
1. Antisera to R-salt-azo-benzidine-azo-crystalline egg albumin give precipitates with crystalline egg albumin by virtue of their antidye content.. 2. The quantitative course of the reactions with increasing amounts of antigen is very similar for the dye-antidye and egg albumin-anti-egg albumin systems, but differs markedly for the cross reaction between egg albumin and antidye.. 3. A possible explanation for the occurrence of this one-sided crossreaction is given in terms of reactive groupings on the antigen and antibody.. 4. A qualitative expression of the course of the cross-reaction is given in terms of the laws of classical chemistry.. ...
Heterosubtypic T cell-mediated immunity to influenza A viruses in humans has been widely reported (24-26, 36). While cross-reactive T cells cannot prevent establishment of infection, they can enhance recovery by promoting virus clearance and reduce the severity of illness (15). It has also been demonstrated that in vitro T cell cultures derived from healthy individuals can lyse target cells infected with swine or avian influenza viruses (28, 29). Together with a large number of studies in mice that demonstrated cross-protection conferred by influenza A-specific T cells during subsequent challenges (reviewed in ref. 11), these studies suggest that vaccines stimulating cross-reactive T cell populations, potentially used in conjunction with the current antibody-based strategy, may confer broader and more effective protection against a potential pandemic strain. However, most studies using T cell cultures expanded in vitro were unable to assess accurately the overall influenza-specific memory T cell ...
Control of swine influenza by vaccination has become more difficult in recent decades, as the evolution of the virus has resulted in inconsistent responses to traditional vaccines. Standard commercial swine flu vaccines are effective in controlling the infection when the virus strains match enough to have significant cross-protection, and custom (autogenous) vaccines made from the specific viruses isolated are created and used in the more difficult cases.[80][81] Present vaccination strategies for SIV control and prevention in swine farms typically include the use of one of several bivalent SIV vaccines commercially available in the United States. Of the 97 recent H3N2 isolates examined, only 41 isolates had strong serologic cross-reactions with antiserum to three commercial SIV vaccines. Since the protective ability of influenza vaccines depends primarily on the closeness of the match between the vaccine virus and the epidemic virus, the presence of nonreactive H3N2 SIV variants suggests that ...
Self-reactive CD4 T cells are thought to have a central role in the pathogenesis of many chronic inflammatory human diseases. Microbial peptides can activate self-reactive T cells, but the structural basis for such crossreactivity is not well understood. The Hy.1B11 T cell receptor (TCR) originates from a patient with multiple sclerosis and recognizes the self-antigen myelin basic protein. Here we report the structural mechanism of TCR crossreactivity with two distinct peptides from human pathogens. The structures show that a single TCR residue (CDR3α F95) makes the majority of contacts with the self-peptide and both microbial peptides (66.7-80.6%) due to a highly tilted TCR-binding topology on the peptide-MHC surface. Further, a neighbouring residue located on the same TCR loop (CDR3α E98) forms an energetically critical interaction with the MHC molecule. These data show how binding by a self-reactive TCR favors crossreactivity between self and microbial antigens.
Full FDA Tissue Panel of high quality normal tissues for antibody cross-reactivity studies. Multiple organs from a range of age and gender-specific donors.
Transglutaminase 2 (TG2) is a ubiquitously expressed enzyme that catalyzes the posttranslational modification of glutamine residues on protein or peptide substrates. 3-bromo-4 5 (DHI) scaffold have been widely used to study TG2 biology and are well tolerated in vivo but these compounds have only modest potency and their selectivity toward other transglutaminase homologues is largely unknown. In the present work we first profiled the selectivity of existing inhibitors against the most pertinent TG isoforms (TG1 TG3 and FXIIIa). Significant cross-reactivity of these small molecules with TG1 was observed. Structure-activity and ?selectivity analyses led to the identification of modifications that improved potency and isoform selectivity. Preliminary pharmacokinetic analysis of the most promising analogues was also undertaken. Our new data provides a clear basis for the rational selection of dihydroisoxazole inhibitors as tools for in vivo biological investigation. Kitl Introduction The mammalian ...
TY - JOUR. T1 - Epidemics in Competition. T2 - Partial Cross-Immunity. AU - Andreasen, Viggo. PY - 2018. Y1 - 2018. N2 - The competition between two pathogen strains during the course of an epidemic represents a fundamental step in the early evolution of emerging diseases as well as in the antigenic drift process of influenza. The outcome of the competition, however, depends not only on the epidemic properties of the two strains but also on the timing and size of the introduction, characteristics that are poorly captured by deterministic mean-field epidemic models. We describe those aspects of the competition that can be determined from the mean-field models giving the range of possible final sizes of susceptible hosts and cumulated attack rates that could be observed after an epidemic with two cross-reacting strains. In the limit where the size of the initial infection goes to zero, the possible outcomes lie on a (one dimensional) curve in the outcome space.. AB - The competition between two ...
Interleukin-5 (IL-5) is a hematopoietic growth factor expressed in Th2, mast cells and eosinophils. IL-5 acts through its receptor, the IL-5 receptor (IL-5R) and is involved in B-cell growth and eosinophil activation. IL-5 has been shown to be and is regulated by GATA-3, in addition to other transcription factors. Human and mouse IL-5 are cross-reactive. Recombinant mouse IL-5 is a non-glycosylated, disulfide-linked homodimer. It is comprised of two 113 amino acid chains with a total molecular weight of 26.2 kDa. Ships ambient. Store frozen at -20 to -10C. ...
A gray area at times. Have used the Scytek kit (check out website) for frozen sections, but did not use their peroxidase blocker, it ate frozens off the slide. Substituted a Dako peroxidase blocker for frozens was much friendlier. The kicker with this kit, their special blocker is excellent, and you have to use their chromogen with this kit. What really worked for us was to use their two blockers (not the peroxidase block though),a biotinylated primary in place of a secondary antibody with the Scytek kit since we did not have a purified primary on hand. We also diluted out the biotinylated primary further, so do a more extended dilution panel, this reduced our cross reaction background. This is the basis of the DAKO ARK kit, biotinylating the primary, some antibodies don biotinylate as well, a potential problem, but may work with yours, so another option - DAKO ARK kit. There are others on the market, Zymed, Innovex, Vector MOM kit, etc, all worth a try. Have heard some mixed reviews on Mouse ...
Species: Mouse Monoclonal Clone: 1A4 Isotype: IgG2a,k Species Reactivity: Human, Baboon, Cow, Rabbit, Mouse, Rat, Chicken. Positive Control: Blood vessels Specificity: This antibody recognizes the a-smooth muscle form of actin. It shows no cross reaction with actin from fibroblasts (b- and g-cytoplasmic), striated muscle (a-sarcometric), and myocardium (a-myocardial). Its epitope is composed of the acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of a-smooth actin. ScyTek s 1A4 stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained as they also contain this actin ...
Species: Mouse Monoclonal Clone: 1A4 Isotype: IgG2a,k Species Reactivity: Human, Baboon, Cow, Rabbit, Mouse, Rat, Chicken. Positive Control: Blood vessels Specificity: This antibody recognizes the a-smooth muscle form of actin. It shows no cross reaction with actin from fibroblasts (b- and g-cytoplasmic), striated muscle (a-sarcometric), and myocardium (a-myocardial). Its epitope is composed of the acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of a-smooth actin. ScyTek s 1A4 stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained as they also contain this actin ...
Although Ag-Ab reactions are highly specific, in some cases antibody elicited by one antigen can cross-react with an unrelated antigen. Such cross-reactivity
Hello, I work with fish in a resarch lab and with some of the antibodies I use I get what I would consider non-specific crossreactivity with mucus either covering the body surface or in mucocyte granules. More often this problem would arise with rabbit polyclonal anti-peptide antibodies using neat serum. I usually get around this by affinity purifying using the peptide but in some cases this is not possible. Someone once told me that mucoproteins have a large number of antigenic determinants increasing the likelyhood of crossreactivity but I could never find a reference. Ive searched through the archives but couldnt find an explanation except for a suggestion that it might be charge related. Could someone please shed some light on this situation for me. It is something that has bugged me for the longest time. Thank you for any insight. Sincerely, Jon _______________________________________________ Histonet mailing list [email protected] ...
Publications 164 Publications PUBLICATIONS 1. Heemskerk B, Veltrop-Duits LA, van Vreeswijk T, ten Dam MM, Heidt S, Toes RE, van Tol MJ, Schilham MW. Extensive cross-reactivity of CD4 + adenovirus-specific
Biomarker studies which were previously limited by poor assay sensitivity, low throughput systems and insufficient sample amount are now feasible in a simple and efficient way.. For the first time it is possible to do high capacity quantification of 92 protein biomarkers in 96 samples. Conventional immunoassays have been unable to scale due to increasing antibody cross-reactivity but TATAA Biocenter offers a solution using the Multiplex platform from Olink Proteomics. It generates high quality data even in highly multiplexed formats and the opportunities are enormous for powerful analysis of multivariate biomarker patterns.. ...
Supplier: Assaypro • Crossreactivity: Bo, Ms, Rt, Pg, Mk, Dg, Rb • Format: Sandwich ELISA • Range: 3.125-200 ng/mL • Min. Sample Size: 50 uL • Sample Types: U, M, Sv, ...
In this thesis the role of bacteria for the induction of autoimmunity was investigated. In detail, it was examined whether bacteria are able to activate autoreactive CD4+-T-cells antigen-specific ("cross-reactivity") or antigen-unspecific ("bystander-activation"). It was shown that the examined transgenic MBP-peptide specific T-cell-receptor recognized many natural occurring cross-reactive peptides of microbial origin, which induced an activation of the T-cells in vitro and which could induce autoimmune encephalomyelitis (EAE) in the T-cell-receptor transgenic mice in vivo. Furthermore, it was examined, whether lipopolysaccharide (LPS) as activator of the innate immune system could induce an unspecific activation of the autoreactive T-cells in vitro and whether administration of LPS in the transgenic mice could induce EAE in vivo. It was shown that LPS activates a small percentage of CD4+ - T-cells. Application of LPS to the transgenic T+alpha- mice induced EAE. Therefore, the role of ...
Despite inheriting half of its genetic material from its father, the fetoplacental unit is not rejected by the maternal immune system because of a unique immunological relationship with the mother
The properties of high affinity and specificity of antibodies are successfully employed in assays used for quantification of specific analytes in complex mixtures. The method is indirect in the sense that it is the antibody-analyte complex that is detected rather than the analyte per se. However, if the quality of the antibody is good, i.e., low cross reactivity with other molecules, there is a strong relationship between the measured signal from the complex and the concentration of the analyte. This relationship can be determined by the use of calibrators with known concentrations, which allow for a standard curve to be constructed and the concentration in a sample to be calculated.. ...
anti-DJ-1 (PARK7), mAb (1D7) is a monoclonal antibody that crossreacts with human, mouse, rat protein. Works in ELISA, WB. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
Citoxlab provides GLP-compliant Tissue Cross-Reactivity (TCR) studies for biologicals and monoclonals with all the tissues recommended by the FDA and EMEA.
anti-BEDF, mAb (PK33-5B8) is a monoclonal antibody that crossreacts with human protein. Works in WB, IP. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
, Rat Serum - 50mL, GTX73216, Applications: Blocking, ELISA, ICC/IF, IHC, WB; Blocking, ELISA, Immunocytochemistry/ Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Western Blot (WB); CrossReactivity:
, Human Brain: Amygdala (Normal) tissue lysate, GTX28766, Applications: ELISA, IP, WB; ELISA, Immunoprecipitation, Western Blot (WB); CrossReactivity:
Newcombe, J, Eales-Reynolds, L, Wootton, L, Gorringe, AR, Funnell, SGP, Taylor, SC and McFadden, JJ (2004) Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity ...
Three anti-horse liver alcohol dehydrogenase (HLADH) monoclonal antibodies are described. Two are specific for ADH and cross-react with class I and II enzymes from mouse, horse and Chinese hamster....
TY - JOUR. T1 - Formalin-inactivated EV71 vaccine candidate induced cross-neutralizing antibody against subgenotypes B1, B4, B5 and C4A in adult volunteers. AU - Chou, Ai Hsiang. AU - Liu, Chia Chyi. AU - Chang, Jui Yuan. AU - Jiang, Renee. AU - Hsieh, Yi Chin. AU - Tsao, Amanda. AU - Wu, Chien Long. AU - Huang, Ju Lan. AU - Fung, Chang Phone. AU - Hsieh, Szu Min. AU - Wang, Ya Fang. AU - Wang, Jen Ren. AU - Hu, Mei Hua. AU - Chiang, Jen Ron. AU - Su, Ih Jen. AU - Chong, Pele Choi Sing. PY - 2013/11/21. Y1 - 2013/11/21. N2 - Background: Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia. No effective EV71 vaccine is available. A randomized and open-label phase I clinical study registered with ClinicalTrials.gov #NCT01268787, aims to evaluate the safety, reactogenicity and immunogenicity of a formalin-inactivated EV71 vaccine candidate (EV71vac) at 5- and 10-μg doses. In this study we report the cross-neutralizing antibody responses from each ...
In a subset of patients with limited cutaneous (lc) systemic sclerosis (SSc), anti-CENP-A antibodies (Ab) cross-react with a peptide (FOXE3p53-62) that presents striking homology with one of the two immunodominant epitopes of CENP-A (Ap17-30). We searched for clinical correlates of anti-FOXE3p53-62 Ab by measuring their levels along with those of Ab to Ap17-30 and to the second immunodominant epitope of CENP-A, namely Ap1-17. Serum samples were obtained from 121 patients with SSc, 46 patients with systemic lupus erythematosus (SLE) and 25 healthy blood donors (HBD). The reactivity of serum IgG to Ap1-17, Ap17-30 and FOXE3p53-62 was measured by ELISA. The corresponding anti-peptide Ab were affinity-purified from pooled SSc sera and used to establish standard curves for quantifying these Ab in patients and HBD. Receiver operating characteristics (ROC) analysis, comparing SSc patients who were positive for anti-CENP Ab (ACA+) to those who were negative, was used to find cut-off points for dichotomizing the
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Abbkine Scientific has announced the launch of its new kit, the EliKine™ Human IL-2 ELISA Kit otherwise known as Human IL2 ELISA Kit. The launch of the product reiterates the companys commitment to enhancing research and investigation in the field of life science.. The product has a human reactivity, employing a two-site sandwich ELISA to quantitate IL-2 in samples. With colorimetric detection method and assay duration with multiple steps standard sandwich ELISA assay with a working time of 3-5 hours, the kit stands tall amongst its peers on the market.. The featured kit includes Human IL-2 microplate, Human IL-2 standard, Human IL-2 detect antibody, EliKine™ Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer and Plate covers.. The major feature and benefit of the kit besides having a calibration range of 3.9 pg/ml-250 pg/ml is its high sensitivity and excellent specificity for detection of Human IL-2. It also has no significant cross-reactivity or ...
Human TNF-α ELISA Kit is the latest product from Abbkine Scientific Research Company. As part of the companys plans to ease the research process and ensure that better results are gotten in record time, the company made the announcement to official launch the Human TNF alpha ELISA Kit on the market.. The ELISA Kit is particularly designed to allow for the easy detection of Human TNF-α. Consequently, investigators, researchers and other such users of the product can easily get their desired results in record time. This also ensures that better results are gotten.. EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples, it has high sensitivity and excellent specificity for detection of Human TNF-α. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.. The recent launch of the Human TNF-α ELISA Kit has been described by many as a revolutionary introduction to the industry. With a high sensitivity and ...
Objectives. This study tested the hypothesis, first proposed by Chaussinand, that individual-level immunity acquired from exposure to tuberculosis may have contributed to the disappearance of leprosy from western Europe. Methods. The epidemiological consequences of cross-immunity were assessed by the formulation of a mathematical model of the...
Swine flu has been in the headlines for days now. The headlines change every day. By the time this article is printed, the situation will have changed even more. Therefore, it is hard to write a timely article on this particular disease. There are some facts that bear no relation to time. The first is that we have flu epidemics every year. They usually occur in the December to February time frame. We start an immunization program for it prior to that. The viruses contained in the immunization are scientists best guesses as to which flu viruses are going to be prevalent that particular year. They usually guess correctly so many people are protected before each years outbreak. One of the issues with swine flu is that there is currently no immunization for it. Cross reaction to other flu strains is not present. Therefore, no one is immune to the virus. For that reason many more people would be affected by an outbreak. The second fact is that every year people who get the flu die from its ...
Centralized" (ancestral and consensus) HIV-1 envelope immunogens induce broadly cross-reactive T cell responses in laboratory animals; however, their potential to elicit cross-reactive neutralizing antibodies has not been fully explored. Here, we report the construction of a panel of consensus subtype B (ConB) envelopes and compare their biologic, antigenic, and immunogenic properties to those of two wild-type Env controls from individuals with early and acute HIV-1 infection. Glycoprotein expressed from full-length (gp160), uncleaved (gp160-UNC), truncated (gp145), and N-linked glycosylation site deleted (gp160-201N/S) versions of the ConB env gene were packaged into virions and, except for the fusion defective gp160-UNC, mediated infection via the CCR5 co-receptor. Pseudovirions containing ConB Envs were sensitive to neutralization by patient plasma and monoclonal antibodies, indicating the preservation of neutralizing epitopes found in contemporary subtype B viruses. When used as DNA vaccines ...
An agglutination test used in the laboratory to diagnose rickettsial diseases. It depends on a nonspecific cross reaction between antibodies produced by the rickettsial infection with the OX-2, OX-19 and OXK antigens of the Gram negative rod, Proteus.. ...
The participants, recruited by advertisements in two daily newspapers, were obese and asthmatic. Inclusion criteria were ability to cope with the study protocol, body mass index 30-42, age 18-60 years, previously diagnosed asthma with a spontaneous diurnal variation or a bronchodilator response of 15% or more, and being a non-smoker or having stopped smoking for two years or more before age 50. Exclusion criteria were pregnancy, history of bulimia or anorexia, unstable angina or arrhythmia, untreated thyroid disease, symptomatic liver or gall bladder disorder, any other severe disease, insulin treatment, systemic steroid treatment, or history of food allergy or of intolerance to any component of the very low energy dietary preparation that would be used in the study-such as soya, fish, chocolate, or lactose. Participants with a history of adverse reactions to peas, beans, or peanuts were excluded because of possible cross reactions to soya protein.3. Telephone interviews were held with 202 ...
Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Killian on positive skin tb test: There is no cross reaction between them. By the way, up to 5% of normal individuals may test positive to ANA test, usually at low titer. for topic: Positive Skin Tb Test
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
A new paper has just appeared in PLoS Medicine on an old topic: whether seasonal influenza vaccines might also cause enough cross-reactivity to protect against H5N1. The basic idea is simple. The immune system sees the surface proteins on the flu virus and makes protective antibodies against them. The major stimulant for this is the hemagglutinin protein (HA), the H part of H5N1. There are 16 different immunological flavors of HA that cross-react very little.
Hi, I have a mouse anti-bovine glutamate dehydrogenase mAb and Im curious whether anyone would know whether it will crossreact with Human GDH. I think the two proteins are fairly similiar but how similiar are humans with cows??? Please send a reply to my E-mail dsho at med.unc.edu Thanx for any thoughts. Moo Moo, Dave Shock ...
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant h, mRNA. (H00004179-R27) - Products - Abnova
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant n, mRNA. (H00004179-R17) - Products - Abnova