... possess identical reactive groups at opposite ends of the protein crosslinker s spacer arm, and can be reacted in a one-step chemical crosslinking reaction.
This invention is related to a thermoplastic crosslinked product obtainable by the crosslinking reaction of a composition comprising (A) a polymer having a silicon-containing group and (B) a tetravalent tin compound, said silicon-containing group having a hydrolyzable group bound to a silicon atom and capable of crosslinking through formation of a siloxane bond.
The CD4 receptor contributes to T-cell activation by coligating major histocompatibility complex class II on antigen presenting cells with the T-cell receptor (TCR)/CD3 complex, and triggering a cascade of signaling events including tyrosine phosphorylation of intracellular proteins. Paradoxically, CD3 cross-linking prior to TCR stimulation results in apoptotic cell death, as does injection of anti-CD4 antibodies in vivo of CD4 ligation by HIV glycoprotein (gp) 120. In this report we investigate the mechanism by which CD4 cross-linking induces cell death. We have found that CD4 cross-linking results in a small but rapid increase in levels of cell surface Fas, a member of the tumor necrosis factor receptor family implicated in apoptotic death and maintenance of immune homeostasis. Importantly, CD4 cross-linking triggered the ability of Fas to function as a death molecule. Subsequent to CD4 cross-linking, CD4+ splenocytes cultured overnight became sensitive to Fas-mediated death. Death was ...
In this work, intramolecular and intermolecular associations under the effect of shear flow of dilute aqueous alkaline solutions of dextan, hydroxyethylcellulose (HEC), and the hydrophobically modified analogue (HM-2-HEC) in the presence of a chemical cross-linking agent were characterized with the aid of viscometry and rheo-small angle light scattering (rheo-SALS) methods. The picture that emerges at short times during the cross-linking reaction at a constant shear rate is that HEC coils contract because of intramolecular cross-linking; whereas the HM-2-HEC species show an incipient association and the dextran molecules are unaffected due to their compact structures. At longer times, interchain cross-linking of the polymer promotes the growth of large flocs, which are disrupted by shear forces when they are sufficiently large. The delicate interplay between intramolecular and intermolecular association is governed by factors such as the magnitude of the shear rate, the cross-linking agent ...
Dried hemoactive materials comprise both a cross-linked biologically compatible polymer and a non-cross-linked biologically compatible polymer. The cross-linked polymer is selected to form a hydrogel when exposed to blood. The non-cross-linked polymer is chosen to solubilize relatively rapidly when exposed to blood. The non-cross-linked polymer serves as a binder for holding the materials in desired geometries, such as sheets, pellets, plugs, or the like. Usually, the cross-linked polymer will be present in a particulate or fragmented form. The materials are particularly suitable for hemostasis and drug delivery.
ChemInform Abstract: Incorporating Disulfide Cross-Links at the Terminus of Oligonucleotides via Solid-Phase Nucleic Acid Synthesis.|span||/span| | S. E. OSBORNE; A. D. ELLINGTON | download | BookSC. Download books for free. Find books
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
B. DNA-Protein Cross-Linking. To cross-link protein to the genomic DNA, 270 µl of formalin was added to 10 ml of DMEM containing acini to obtain a final solution of 1% formaldehyde. Cells were agitated for 10 minutes (Note 1) on a shaker table. Following the addition of 514 µl of 2.5 M glycine (125 mM final) for 5 minutes to quench the formaldehyde and terminate the crosslinking reaction, cells were centrifuged at 2000 rpm for 3 minutes at 40C. The pellet of acinar cells was washed twice with ice cold PBS.. C. Isolation of Nuclei from Isolated Acinar Cells. To isolate nuclei, acinar cells were incubated in Cytoplasmic Extract buffer (CE Buffer; 10 mM HEPES pH 7.4, 10 mM KCl, 1.5 mM MgCl2, 0.1% Triton-X100, 0.5 mM DTT and protease inhibitors [5 µg/mL Aprotinin, 5 µg/mL Leupeptin, 5 µg/mL Pepstatin, 75 µg/mL PMSF]) followed by 5 strokes of Potter-Elvehjem homogenizer. The cells were set on ice for 15 minutes. To pellet the nuclei, the samples were centrifuged at 4oC and 5000 g for 10 ...
Methods are provided for making bispecific antibodies and antibody conjugates comprising site-specifically cross-linking two or more antibodies, antibody fragments or Fc-fusion proteins. Also provided
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We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. Görlich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (Görlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that ...
SIA crosslinker is a non-cleavable, heterobifunctional protein crosslinker. SIA crosslinking reagent is the shortest amine and sulfhydryl (thiol) reactive protein crosslinker.
Using chemical cross-linkers to map contacts among amino acids, structural biologists predict that soluble tau is, in fact, a compact globule containing β-sheets poised to snap into a pathological formation.. ...
Hello, I am attempting to perform some experiments that inwolve cross-linking with Pierce photoreactive bifunctional cross-linker APDP. As I have no practice using that reagent, any advice would be most welcome. I plan to label one protein via sulphydryl end of APDP and then use the labelled protein in a reaction. After that the glycerol gradient will be run, and appropriate fractions will be flashed with light to activate the photoreactive group of APDP and cross-link that protein to whatever it has in proximity. I have read the papers provided by Pierce, but still have some questions, as follows: - Does APDP decompose thermally readily? Will it be possible to heat APDP-bound protein (that first one) for some 10 min, or will it destroy the photoreactive end (as I am affraid)? And how long can I keep APDP-bound protein sample before actual cross-linking with flashlight? - How photosensitive it is? Do I have to worry about ambient light when labelling the first protein by sulphydryl? - During the ...
Current hydrogels based on chondroitin sulfate (ChS) generally lack the necessary strength and precise mechanical tunability. Addressing these limitations, covalent cross-linking has evolved to produce hydrogels with desirable properties. However, such a methodology always precludes injection and self-healin
The present invention relates to a method of cross-linking a polymeric carbohydrate material with a second material by means of a soluble carbohydrate polymer and a crosslinking agent. The present invention furthermore relates to the resulting cross-linked material, to uses of the cross-linked material, as well as to a kit comprising the soluble carbohydrate polymer and the cross-linking agent. ...
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PRODUCTO ETX Cross-linking agent that improves attachment and wash fastness of resins. PRODUCTO R3 conc. Self-cross-linking agent of resin that improves wash and rubbing fastness of pigments.
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SF3B4 is one of four subunits of the splicing factor 3B. The protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint…
Tris-EDTA is used to dilute and store nucleic acid samples. Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE Buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
There is provided herein a membrane or film comprising one or more aromatic ionomers covalently crosslinked through aryl-aryl (--Ar--Ar--), aryl-ether-aryl (--Ar--O--Ar--), aryl-sulfide-aryl (--Ar--S--Ar--), aryl-sulfone-aryl bonds, or any combination thereof, wherein said one or more aromatic ionomers further comprises at least one electron withdrawing group adapted to improve oxidant resistance of said membrane or film.
WESTBURY, NY--(Marketwired - September 11, 2015) - The crosslinking process utilizes short wave (254 nm) UV light to covalently bond nucleic acids to either nitrocellulose or nylon membranes. Spectronics Corporation manufactures advanced, versatile and accurate crosslinkers to avoid the loss of nucleic acids during the hybridization process, a consistent, irritating...
Bio-Synthesis offers peptide conjugation using various cross-linking chemistries. Carrier protein such as KLH, BSA, OVA can be conjugated to peptides.
1XW7: Diabetes-associated mutations in human insulin: crystal structure and photo-cross-linking studies of a-chain variant insulin wakayama
The Spectrolinker XL-1000 UV crosslinker was made for applications including eliminating PCR contamination, cross-linking DNA an...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
When a low concentrations of crosslinker is added to resist (a film used to lay down the patterns of ever-shrinking lines and features on a chip, shown here at left), it is able to pattern smaller ...
TY - JOUR. T1 - Intramolecular crosslinking of hemoglobin A by sulfosuccinimidyl suberate. T2 - Application of crosslinked protein as a blood substitute. AU - Manjula, B. N.. AU - Acharya, A. S.. PY - 1994/11. Y1 - 1994/11. N2 - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the appropriate length of the alkyl chain optimal for the crosslinking reaction.. AB - Sulfosuccinimidyl esters could be targeted to the residues of ββ cleft of hemoglobin A and the bis-sulfosuccinimidyl esters of aliphatic dicarboxylic acids could serve as the `affinity directed ββ crosslinkers of HbA. The reactivity of sulfosuccinimidyl esters of tartaric acid and sebacic acid with HbA was also investigated to establish the ...
ABCA formed a novel intramolecular cross-linking adduct on human serum albumin in patients and in vitro via Michael addition, followed by nucleophilic adduction of the aldehyde with a neighbouring protein nucleophile. Adducts were detected on lysine, histidine, and cysteine residues in subdomain IB of human serum albumin. Only a cysteine adduct and a putative cross-linking adduct were detected on glutathione S-transferase Pi. Modelling the docking of ABCA with HLA B*57:01 confirmed that ABCA has a strong binding affinity when bound covalently to Ser116, a key residue with regards to recognition by ABC-specific CD8+ T cells. ...
215597-96-9,CCD No.:CCD00211814,Formula:C12 H10 N2 O14 S2 . 2 Na,MolWeight:516.323,Synonyms:BIS(SULFOSUCCINIMIDYL)SUCCINATE SODIUM SALT; Molecular Structure,Chemical Cloud Database
Sigma-Aldrich offers bifunctional and trifunctional linkers and crosslinking reagents for various conjugation applications.

There are numerous bioconjugation possibilities with our linkers and crosslinkers, offering potential for structural stability or assistance in protein-protein, protein-peptide and peptide/protein-small molecule interactions. Other applications include immobilization for assays or purification, as well as various peptide-nucleic acid and nucleic acid-nucleic acid conjugations, among many others.

Our homo- and heterofunctional linkers contain diverse functional groups, such as primary amines, sulfhydryls, acids, alcohols and bromides. Many of our crosslinkers are functionalized with maleimide (sulfhydral reactive) and succinimidyl ester (NHS) or isothiocyanate (ITC) groups that react with amines. A selection of mono-protected (Boc, Fmoc, and Cbz) linkers is also available.
Reggie-1 and -2 proteins (flotillin-2 and -1 respectively) form their own type of non-caveolar membrane microdomains, which are involved in important cellular processes such as T-cell activation, phagocytosis and signalling mediated by the cellular prion protein and insulin; this is consistent with the notion that reggie microdomains promote protein assemblies and signalling. While it is generally known that membrane microdomains contain large multiprotein assemblies, the exact organization of reggie microdomains remains elusive. Using chemical cross-linking approaches, we have demonstrated that reggie complexes are composed of homo- and hetero-tetramers of reggie-1 and -2. Moreover, native reggie oligomers are indeed quite stable, since non-cross-linked tetramers are resistant to 8 M urea treatment. We also show that oligomerization requires the C-terminal but not the N-terminal halves of reggie-1 and -2. Using deletion constructs, we analysed the functional relevance of the three predicted ...
Dear netter, I am trying to cross-link some of my mutated actin monomers within filament actin by using the zero-length cross-linker. Anybody has success with this cross-linker? Your protocol and advice will be highly appreciated. Bing ...
TY - JOUR. T1 - Sulfhydryl Site-Specific Cross-Linking and Labeling of Monoclonal Antibodies by a Fluorescent Equilibrium Transfer Alkylation Cross-Link Reagent. AU - del Rosario, Renato B.. AU - Wahl, Richard L.. AU - Brocchini, Stephen J.. AU - Lawton, Richard G.. AU - Smith, Richard H.. PY - 1990/1/1. Y1 - 1990/1/1. N2 - The site-specific intramolecular cross-linking of sulfhydryls of monoclonal antibodies via a new class of "equilibrium transfer alkylation cross-link (ETAC) reagents" is described. Following complete or partial reduction of interchain disulfides with dithiothreitol (DTT), two murine IgG2a monoclonal antibodies, 225.28S and 5G6.4, were reacted with a,a-bis[(p-tolylsulfonyl)methyl]-m-aminoacetophenone (ETAC 1a) and a fluorescent conjugated derivative, sulforhodamine B m-(α,α-bis(p-tolylsulfonylmethyl) acetyl)anilide derivative (ETAC 1b). Reducing SDS-polyacrylamide gel electrophoresis analysis of the products from lb indicated the formation of S-ETAC-S interchain heavy and ...
Thermo Scientific™ Pierce™ DSS Crosslinker 1g Thermo Scientific™ Pierce™ DSS Crosslinker NHS-ester Crosslinkers
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Enzymes are immobilized by dissolving in water a photo-crosslinking resin containing stilbazolium groups, vinyl alcohol units and vinyl acetate units, adding an enzyme to the resultant aqueous solution and exposing the enzyme-containing resin solution to light to induce a crosslinking reaction of the photo-crosslinking resin and produce a polymer containing the enzyme entrapped therein.
It has been suggested that GAP-43 (growth-associated protein) binds to various proteins in growing neurons as part of its mechanism of action. To test this hypothesis in vivo, differentiated N1E-115 neuroblastoma cells were labeled with [35S]-amino acids and were treated with a cleavable crosslinking reagent. The cells were lysed in detergent and the lysates were centrifuged at 100,000 x g to isolate crosslinked complexes. Following cleavage of the crosslinks and analysis by two-dimensional gel electrophoresis, it was found that the crosslinker increased the level of various proteins, and particularly actin, in this pellet fraction. However, GAP-43 was not present, suggesting that GAP-43 was not extensively crosslinked to proteins of the cytoskeleton and membrane skeleton and did not sediment with them. GAP-43 also did not sediment with the membrane skeleton following nonionic detergent lysis. Calmodulin, but not actin or other proposed interaction partners, co-immunoprecipitated with GAP-43 from the
TY - JOUR. T1 - Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. AU - Wang, Jin. AU - Zhang, Da Wei. AU - Lei, Ying. AU - Xu, Fang. AU - Cohen, Jonathan C.. AU - Hobbs, Helen H.. AU - Xie, Xiao Song. PY - 2008/5/6. Y1 - 2008/5/6. N2 - ABCG5 (G5) and ABCG8 (G8) are ATP-binding cassette half-transporters that limit intestinal uptake and promote biliary secretion of neutral sterols. Here, we describe the purification of endogenous G5G8 from mouse liver to near homogeneity. We incorporated the native proteins into membrane vesicles and reconstituted sterol transfer. Native gel electrophoresis, density-gradient ultracentrifugation, and chemical cross-linking studies indicated that the functional native complex is a heterodimer. No higher order oligomeric forms were observed at any stage in the catalytic cycle. Sterol transfer activity by purified native G5G8 was stable, stereospecific, and selective. We also report that G5 but not G8 is S-palmitoylated and that ...
Summary. Background: Activated factor XIII (FXIIIa), a transglutaminase, introduces fibrin-fibrin and fibrin-inhibitor cross-links, resulting in more mechanically stable clots. The impact of cross-linking on resistance to fibrinolysis has proved challenging to evaluate quantitatively. Methods: We used a whole blood model thrombus system to characterize the role of cross-linking in resistance to fibrinolytic degradation. Model thrombi, which mimic arterial thrombi formed in vivo, were prepared with incorporated fluorescently labeled fibrinogen, in order to allow quantification of fibrinolysis as released fluorescence units per minute. Results: A site-specific inhibitor of transglutaminases, added to blood from normal donors, yielded model thrombi that lysed more easily, either spontaneously or by plasminogen activators. This was observed both in the cell/platelet-rich head and fibrin-rich tail. Model thrombi from an FXIII-deficient patient lysed more quickly than normal thrombi; replacement ...
Tissue transglutaminase (tTG) belongs to the family of transglutaminase enzymes that catalyze the posttranslational modification of proteins via Ca(2+)-dependent cross-linking reactions. The catalytic action of tTG results in the formation of an isopeptide bond that is of great physiological significance since it is highly resistant to proteolysis and denaturants. Although tTG-mediated cross-linking reactions have been implicated to play a role in diverse biological processes, the precise physiological function of the enzyme remains unclear. Recent data, however, suggest that the protein polymers resulting from tTG-catalyzed reactions may play a role in commitment of cells to undergo apoptosis. On the same token, tTG-mediated formation of insoluble protein aggregates may underlie the markers of numerous pathological conditions, such as the senile plaques in Alzheimers disease and the Lewy bodies in Parkinsons disease. In addition to catalyzing Ca(2+)-dependent cross-linking reactions, tTG can also
Tissue engineering typically requires a use of scaffolds when delivering tissue-specific cells to be engineered. Hydrogels are frequently used as scaffolds, because their composition, structure, and function resemble the natural tissue extracellular matrix. In this study, hyaluronate-alginate hybrid (HAH) was synthesized by conjugating alginate (ALG) with the hyaluronate (HA) backbone using various types of linkers. HAH hydrogel was prepared by physically cross-linking the HAH polymer in the presence of calcium ions without chemical cross-linkers. The mechanical stiffness of HAH hydrogel was significantly affected by changing the type of a linker between HA and ALG. The mechanical stiffness increased with increasing linker length, likely due to enhanced intermolecular reactions between HA and ALG. This enables controlling the mechanical properties of HAH hydrogels. The types of linkers used to synthesize HAHs also influenced the chondrogenic differentiation of ATDC5 cells cultured in HAH ...
2005 (English)In: 8th International Symposium of Polymers for Advanced Technologies, 13th-16th Sept. 2005, Budapest, Hungary, 2005Conference paper, Published paper (Other scientific) ...
Polyamides comprising substantial amounts of Nylon-11 and/or Nylon-12 units are cross-linked by irradiation in the presence of an unsaturated cross-linking agent, preferably triallyl isocyanurate. The cross-linked products are particularly useful in the form of heat-recoverable shaped articles, e.g. heat-shrinkable tubing.
In order for transplanted cells to survive in the body, they need to be gently implanted, as well as provide a suitable environment allowing them to attach, survive, grow and function. Because different cell types prefer different types of environments, the delivery matrix can be modified. The HyStem® hydrogels permit gentle implantation by supporting cells when they are injected through a needle. HyStem® hydrogels are highly customizable, allowing for a variation of gel stiffness, viscosity and gelation time. They are also biocompatible. BioTime has designed these hydrogels to dissolve when needed to gently release cells from the matrix.. In addition to cells, proteins and/or other biological factors it can also be used as a time-release depot for drugs or biological molecules.. The technology underlying BioTimes HyStem® hydrogels was developed at the University of Utah and is based on a unique chemical cross-linking strategy. Building upon this platform, BioTime has developed the HyStem ...
Microcapsules are formed in the absence of coacervation by providing an oil-in-water emulsion containing a polymeric, emulsifying agent having cross-linkable groups or complexing sites and admixing with the emulsion a cross-linking agent or a complexing agent which forms an impermeable coating around the dispersed oil droplets. The emulsifying agent may be non-proteinaceous or the protein, gelatin. Impermeable capsule walls are formed solely by the addition of the cross-linking or complexing agent and extraneous hardening agents are obviated. Moreover, the emulsifying agent may be a preformed, polymeric, cross-linking agent which eliminates the need for any separate cross-linking agent.
A number of clinically important antitumor agents such as cisplatin, cyclophosphamide (a nitrogen mustard) or carmustine (BCNU, a chloro ethyl nitroso urea) for...
556 The pyrrolo[2,1-c][1,4]benzodiazepines (PBDs) are naturally occurring antitumor antibiotics isolated from various Streptomyces species. They bind covalently in the minor groove of DNA at purine-guanine-purine motifs. SJG-136, a pyrrolobenzodiazepine dimer based on two PBD units joined through their C8-positions via a propyldioxy linker, is presently in Phase I evaluation in both the UK (through Cancer Research UK) and the USA (through the NCI) as a result of its striking in vitro and in vivo activity. As it contains two electrophilic centers, SJG-136 forms interstrand cross-links between guanines on opposite strands of DNA at Purine-GATC-Pyrimidine sequences. More recently we have explored the synthesis and biological activity of C2-aryl substituted PBD monomers that monoalkylate guanines in the minor groove of DNA rather than form cross-links. In this respect, their mechanism of action is similar to Ecteinascidin-743 (Yondelis, ET-743). We have found that these molecules possess encouraging ...
5-(hexyloxy)psoralen: forms a conjugate with oligonucleotide primers (at its omega-hexyl position) to promote site-specific photo-induced cross-linking at the end of the DNA fragment of interest before analysis by DGGE
Crosslinkable compositions contain a calcium carbonate-rich filler coated with a carboxylic acid of the formula R.sup.4--(OCR.sup.3.sub.2--C(.dbd.O)OH).sub.y. The compositions exhibit low modulus, good adhesion to substrates, and low skin formation time.
To understand outcomes, traditional data collection will only take you so far. IQVIA offers methods and expertise that take you to where you want to be. Explore our Innovative data collection techniques applied to a wide range of sources, cross-linking data and closing gaps. Use classical observational, low interventional clinical studies, or novel hybrid approaches such as mosaic and enriched studies. Designed to generate actionable insights and give you validated, representative, and fast results. Discover our track record in EMA-accepted Drug Utilization Studies (DUS) in multiple therapy areas and geographies and our experience in working with the FDA, ENCePP, EUnetHTA , MHRA.. ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Cross-links are bonds that link one polymer chain to another. These bonds can either be covalent or ionic in nature. In polymer science, the use of cross-links to promote a difference in a polymers physical properties is referred to as cross-linking. When polymer chains are linked together by cross-links, they lose some of their ability to move as individual polymer chains. For example, a liquid polymer, which possesses freely flowing chains can be turned into a "solid" or "gel" by cross-linking the chains together. Cross-linking inhibits close packing of the polymer chains, preventing the formation of crystalline regions. The restricted molecular mobility of a cross-linked structure limits the extension of the polymer material under loading. This means that when a polymer is stretched, the cross-links prevent the individual chains from sliding past each other. In the process, the chains may straighten out, but once the stress is removed they return to their original position and the object ...
A highly stable lipase from Geobacillus thermocatenolatus (BTL2) and the enhanced green fluorescent protein from Aquorea victoria (EGFP) were recombinantly produced N-terminally tagged to the lectin domain of the hemolytic pore-forming toxin LSLa from the mushroom Laetiporus sulphureus. Such a domain (LSL 150), recently described as a novel fusion tag, is based on a β-trefoil scaffold with two operative binding sites for galactose or galactose-containing derivatives. The fusion proteins herein analyzed have enabled us to characterize the binding mode of LSL 150 to polymeric and solid substrates such as agarose beads. The lectin-fusion proteins are able to be quantitatively bound to both cross-linked and non-cross-linked agarose matrixes in a very rapid manner, resulting in a surprisingly dynamic protein distribution inside the porous beads that evolves from heterogeneous to homogeneous along the postimmobilization time. Such dynamic distribution can be related to the reversible nature of the ...
Water swellable polyacrylate articles are made from a solution of the polyacrylate having an effective amount of a soluble crosslinking agent therein by heating and/or drying the solution. The polyacrylate solution is made from a polyacrylate by saponification and the cross-linking agent is then added.
Ridder, A.N.J.A.; Spelbrink, R.E.J.; Demmers, J.A.A.; Rijkers, D.T.S.; Liskamp, R.M.J.; Brunner, J.; Heck, A.J.R.; de Kruijff, B.; Killian, J.A ...
Bifunctional crosslinker reagents for the conjugation of different biomolecules, including proteins, peptides, and oligonucleotides.
A method of decorating laminated glass is disclosed. A pigment package composition comprising a cross-linkable thermoset resin, a crosslinker capable of crosslinking the thermoset resin, and a pigment is applied to a glass substrate. The crosslinker and thermoset resin are cured at a relatively low temperature (ca. 400 F.). The use of an organic based pigment composition allows use of thinner glass sheets than with traditional ceramic enamel pigment compositions.
Thermo Scientific™ Pierce™ BS2G-d0 and BS2G-d4 Crosslinker Mass Pair Heavy analog Thermo Scientific™ Pierce™ BS2G-d0 and BS2G-d4...
Covalent cross-links hold many collagen molecules side by side, forming fibres.The ends of the parallel molecules are staggered.If they were not, there would be a weak spot running right across the collagen fibre.What is the meaning of staggered ends here? Why would there be a weak spot running right across the collagen fibre if the ends are not staggered? Thank you ...
The present invention relates to the penetrating networks comprising a covalently crosslinked polymer component and a polyester urethane component. The materials in accordance with the present invention are suitable in particular as materials for medicinal purposes, as implants, for target controlled stimuli-sensitive drug release, as ligament augmentation and as replacement material for inter-vertebrae disks.
Belotero Lips Contour is an advanced lip filler processed using a unique cross-linking method to produce a cohesive polydensified matrix product.
The most common schemes for forming a well-defined heteroconjugate involve the indirect coupling of an amine group on one biomolecule to a thiol group on a second biomolecule, usually by a two- or three-step reaction sequence.
DNA-protein crosslinks (DPCs) are under-investigated DNA lesions caused by the covalent attachment of proteins to DNA. DPCs are induced by various endogenous chemicals like aldehydes or by chemotherapeutic drugs. Little is known about how cells repair DPCs and thus acquire resistance to DPC-induced chemotherapy. However, the persistence of DPCs causes genomic instability and cancer. We recently discovered a human syndrome (Ruijs-Aalfs or SPARTAN syndrome) related to the defective DPC repair pathway.
Adikaram, P. R., Zhang, J.-H., Kittock, C. M., Pandey, M., Hassan, S. A., Lue, N. G., … Simonds, W. F. (2019). Development of R7BP inhibitors through cross-linking coupled mass spectrometry and integrated modeling. Communications Biology, 2(338). doi: 10.1038/s42003-019-0585- ...
สาริกานนท์, จำลอง; สนธิสมบัติ, อภิชาติ (Rajamangala University of Technology Phra Nakhon, 2009-03-01) ...
TEGO® Rad 2700 is a radically cross-linkable release additive, providing utmost release and slip properties. Additional defoaming properties. Ideal for high-viscosity pigmented inks. ...
The majority of biopharmaceutical proteins are used as is. However, the use of existing and emerging biopharmaceutical proteins can be greatly expanded, if they are derivatized with useful payloads. The resulting conjugates can be used for targeted delivery of therapeutic and diagnostic agents, development of protein-derivatized biomedical surfaces and scaffolds, and for optimization of protein formulation and pharmacokinetic. Unfortunately, the progress in this field has been very slow. The fundamental problem has been the lack of an efficient technology for making protein-based conjugates. Most often conjugation relies on a random chemical cross-linking of a payload directly to a protein. Such process yields a mixture of products with varying activity, biodistribution, and pharmacokinetic. Alternatively, a cysteine residue is engineered into a safe position in the protein and its thiol group is used for site-specific cross-linking. However, the latter approach requires sophisticated ...
Argyris Politis is the author of this article in the Journal of Visualized Experiments: Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
An international team led by scientists at the National Institutes of Health is the first to discover a new way that cells fix an important and dangerous type of DNA damage known as a DNA-protein cross-link (DPC). The researchers ...
Artemis (phospho Ser516) antibody (DNA cross-link repair 1C) for IHC-P, WB. Anti-Artemis (phospho Ser516) pAb (GTX32292) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Artemis antibody (DNA cross-link repair 1C) for ELISA, IHC-P, WB. Anti-Artemis pAb (GTX23834) is tested in Human samples. 100% Ab-Assurance.
Plasmid pDULE-ABK from Dr. Peter Schultzs lab contains the inserts tRNA synthetase and tRNA for a photocrosslinking amino acid and is published in Chembiochem. 2011 Aug 16;12(12):1854-7. doi: 10.1002/cbic.201100194. Epub 2011 Jun 15. This plasmid is available through Addgene.
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Get quotes fast and Choose from 1 clinic(s) offering Corneal CrossLink (CXL) treatment in Coahiula Get quotes fast & choose the best with phone numbers, reviews, prices, maps and pictures. | Page 0
TY - JOUR. T1 - Preparation of cross-linked chitosan microspheres by spray drying. T2 - Effect of cross-linking agent on the properties of spray dried microspheres. AU - Desai, K. G H. AU - Park, Hyun Jin. PY - 2005/6/1. Y1 - 2005/6/1. N2 - Chitosan microspheres cross-linked with three different cross-linking agents viz, tripolyphosphate (TPP), formaldehyde (FA) and gluteraldehyde (GA) have been prepared by spray drying technique. The influence of these cross-linking agents on the properties of spray dried chitosan microspheres was extensively investigated. The particle size and encapsulation efficiencies of thus prepared chitosan microspheres ranged mainly between 4.1-4.7 μm and 95.12-99.17%, respectively. Surface morphology, % erosion, % water uptake and drug release properties of the spray dried chitosan microspheres was remarkably influenced by the type (chemical or ionic) and extent (1 or 2%w/w) of cross-linking agents. Spray dried chitosan microspheres cross-linked with TPP exhibited ...
Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3′-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents. Analysis of the cross-linked products by SDS/PAGE in the absence of reducing agents revealed that 125I-haemopexin was cross-linked specifically to a protein of apparent molecular mass 85-90 kDa. Upon reduction, haemopexin remained cross-linked to a protein of 20 kDa, suggesting that the murine haemopexin receptor has a subunit structure. Two subunits were identified: alpha (p65) and beta (p20). Furthermore, because haemopexin was cross-linked by all three agents to p20, the shortest cross-linker arm being 1.1 nm (11 A), we propose that the haem-haemopexin-binding site resides on this subunit. In addition, a cysteine residue of p20 is located near the ...
TY - JOUR. T1 - Analysis of chromatin structure by in vivo formaldehyde cross-linking. AU - Orlando, Valerio. AU - Strutt, Helen. AU - Paro, Renato. PY - 1997/2. Y1 - 1997/2. N2 - Recent advances leave no doubt that higher order chromatin structures play a fundamental role in many developmentally important mechanisms of gene regulation. In particular analyses in genetic model systems like yeast and Drosophila uncovered novel proteins that are involved in the regulation of chromatin structures. Many of these proteins do not bind directly to DNA but interact in large multimeric complexes. To identify the DNA elements regulated by these multiprotein complexes, alternative approaches to the standard methods of DNA-protein analysis had to be devised. Here we present a method that preserves the architecture of the higher order chromatin structures by crosslinking cells in vivo with formaldehyde. An immunoprecipitation strategy is then used to identify the DNA targets of chromosomal proteins of ...
0024] In various embodiments, the components of the dry adhesive or the components of the shape memory polymer may include a rigid epoxy and a flexible epoxy. The range of possible crosslinking chemistries which may be used to achieve a dry adhesive or shape memory polymer may include alpha, omega-diaminoalkanes, organic multi-carboxylic acid, anhydride, or catalytic (as in imidazole type) crosslinking reactions. There are many different ways to achieve the appropriate relationships between the molecular properties. For example, the dry adhesives or shape memory polymers may include a rigid epoxy, an epoxy extender, and a crosslinking agent; or a rigid epoxy, a flexible crosslinking agent, and a flexible epoxy; or a rigid epoxy, a rigid crosslinking agent, and a flexible epoxy; or a rigid epoxy, a flexible epoxy, and a catalytic curing agent; or a rigid epoxy, a crosslinking agent, and a diluent; or a flexible epoxy, a crosslinking agent, and a diluent; or a rigid epoxy and a flexible ...
Purpose : To evaluate the stromal corneal findings in 3 different cross-linking techniques (epi-off, trans-epithelial and iontophoresis-assisted). Methods : 18 eyes of 18 keratoconus patients (group A) were treated by epi-off corss-linking technique, 15 eyes of 15 keratoconus patients (group B) were treated by trans-epithelial crosslinking technique and 22 eyes of 26 keratoconus patients (group C) were treated by iontophoresis-assisted cross-linking technique. All eyes were examined with the confocal microscope (Confoscan 4, Fortune Technologies, Italy), a central scan of the total corneal thickness was taken before, 1 week and 1 month after cross-linking treatment. Corneal stroma was evaluated by using the Navis v. 3.5.0. Software (NIDEK, Multi-Instrument Diagnostic System, Japan). Results : In group A we found, after cross-linking treatment, activated keratocites from superficial stroma to 275.1 ± 85.9 µm stromal depth at 1 week and from superficial stroma to 324.9 ± 66.0 µm stromal depth ...
To identify the molecular components of the vasoactive intestinal peptide (VIP) binding sites in the liver, 125I-labelled VIP was covalently linked to liver membranes by using the cleavable cross-linker dithiobis(succinimidylpropionate). Purified rat liver plasma membranes were incubated with 125I-VIP, washed and treated with 1 mM-cross-linker. Polyacrylamide-gel electrophoresis of membrane proteins followed by autoradiography revealed a major 125I-VIP-protein complex of Mr 51 000. A minor Mr 89 000 complex was also observed. An identical pattern of protein labelling was obtained using crude membranes from rat liver. Labelling of the Mr 51 000 and 89 000 species was specific in that it could be abolished by native VIP, but was unaffected by 1 microM-glucagon and cholecystokinin octapeptide. Densitometric scanning of autoradiographs indicated that the labelling of the two species was abolished by similar low VIP concentrations (0.1-100 nM). It was also reduced by two VIP agonists, peptide ...
TY - JOUR. T1 - Ruthenium-catalyzed photo cross-linking of fibrin-based engineered tissue. AU - Bjork, Jason W.. AU - Johnson, Sandra L.. AU - Tranquillo, Robert T. PY - 2011/4/1. Y1 - 2011/4/1. N2 - Most cross-linking methods utilize chemistry or physical processes that are detrimental to cells and tissue development. Those that are not as harmful often do not provide a level of strength that ultimately meets the required application. The purpose of this work was to investigate the use of a ruthenium-sodium persulfate cross-linking system to form dityrosine in fibrin-based engineered tissue. By utilizing the tyrosine residues inherent to fibrin and cell-deposited proteins, at least 3-fold mechanical strength increases and 10-fold stiffness increases were achieved after cross-linking. This strengthening and stiffening effect was found to increase with culture duration prior to cross-linking such that physiologically relevant properties were obtained. Fibrin was not required for this effect as ...
Autori: Moldovan GL, DAndrea AD. Editorial: Cell, 139, p.1222-1224, 2009.. Rezumat:. Left unrepaired, DNA interstrand crosslinks represent impassable hurdles for DNA replication, and their removal is a complicated stepwise process involving a variety of enzymes. In a recent paper in Science, Knipscheer et al. (2009) demonstrate that the Fanconi Anemia protein FANCD2 promotes multiple steps of the crosslink repair process. Cuvinte cheie: DNA repair, Fanconi Anemia, DNA replication, cell cycle regulation, cancer URL: http://www.cell.com/abstract/S0092-8674(09)01553-0. ...
Photo cross-linkable thermoresponsive polymers of UCST-type based on acrylamide (AAm) and acrylonitrile (AN) useful for preparing thermophilic hydrogel films and fibers are presented. The polymers prepared via free radical and reversible addition fragmentation chain-transfer (RAFT) polymerization methods usi
Corneal collagen cross-linking successfully halted the progression of keratoconus for up to 4 years or more, according to a study.Investigators analyzed 30 patients with early to moderate keratoconus who underwent unilateral corneal collagen cross-linking with riboflavin and ultraviolet A 4 years to 6 years previously. Mean age at the time of cross-linking was 26.3 years; the mean interval between cross-linking and clinical evaluation was 53.3 months. Full Story →. ...
D2647 - 10 Standard Specification for Crosslinkable Ethylene Plastics , crosslinkable, crosslinked, ethylene plastics, insoluble fraction, volume resistivity, Crosslinking, Ethylene plastics--specifications,
In this article, Non-Tin Catalysts for Crosslinkable Silane-Terminated Polymers, published in the February 2016 issue of Coatings Tech, John Florio and Ravi Ravichandran discuss new tin-free compounds that have been developed for the catalysis of crosslinkable silane-terminated polymer systems. Each catalyst demonstrates superior activity with different organosilane chemistries used for a variety of applications, such as adhesives, sealants, and coatings. Read the full article here.. ...
0021] The polymerization may additionally be carried out in the presence of at least one crosslinker. In that case copolymers are obtained which have a higher molar mass than if the anionic monomers are polymerized in the absence of a crosslinker. Incorporating a crosslinker into the polymers results, moreover, in reduced solubility of the polymers in water. Depending on the amount of crosslinker copolymerized, the polymers become insoluble in water, but are swellable in water. Crosslinkers which can be used are all compounds which possess at least two ethylenically unsaturated double bonds in the molecule. Examples of crosslinkers are triallylamine, pentaerythritol triallyl ether, pentaerythritol tetraallyl ether, methylene bisacrylamide, N,N-divinylethyleneurea, allyl ethers comprising at least two allyl groups or vinyl ethers comprising at least two vinyl groups, of polyhydric alcohols such as, for example, sorbitol, 1,2-ethanediol, 1,4-butanediol, trimethylolpropane, glycerol, diethylene ...
Pervaporation and gas separation performances of polymer membranes can be improved by crosslinking or addition of metal-organic frameworks (MOFs). Crosslinked copolyimide membranes show higher plasticization resistance and no significant loss in selectivity compared to non-crosslinked membranes when exposed to mixtures of CO2/CH4 or toluene/cyclohexane. Covalently crosslinked membranes reveal better separation performances than ionically crosslinked systems. Covalent interlacing with 3-hydroxypropyldimethylmaleimide as photocrosslinker can be investigated in situ in solution as well as in films, using transient UV/Vis and FTIR spectroscopy. The photocrosslinking yield can be determined from the FTIR-spectra. It is restricted by the stiffness of the copolyimide backbone, which inhibits the photoreaction due to spatial separation of the crosslinker side chains. Mixed-matrix membranes (MMMs) with MOFs as additives (fillers) have increased permeabilities and often also selectivities compared to the pure
[80 Pages Report] Check for Discount on Global Crosslinking Agent Market Research Report 2011-2023 report by HeyReport. Summary The global Crosslinking Agent market will reach xxx Million...
We have devised a generally applicable strategy for analysis of protein structure and have applied it to examine the structure of the transmembrane portion of the Tar receptor of Escherichia coli. The basis of our approach is the use of disulfide cross-linking to identify residues that are within close proximity. To generate and test large numbers of cysteine pairs, we used an unusual method of mutagenesis by which cysteine substitutions can be created randomly at a number of targeted codons. Cysteine-substituted proteins encoded by mutagenized genes may be screened directly for disulfide formation within oligomers or, alternatively, different pools of genes may be randomly recombined to generate gene populations with substitutions in multiple regions. Thus, it is possible to detect a variety of disulfide cross-links between and within individual protein molecules. Interactions between the four membrane-spanning stretches of the Tar dimer were probed by measuring the tendency of 48 cysteine ...
The in vivo association of histone H1 with specific genes in Tetrahymena thermophila was studied by using a simplified cross-linking and immunoprecipitation technique. Four genes were analyzed whose activities vary in three different developmental states (logarithmic growth, starvation, and conjugation). Hybridization of the immunoprecipitated DNA to cloned probes showed an inverse correlation between the level of immunoprecipitation with H1 antiserum and transcriptional activity. This represents the first demonstration of an alteration in histone H1-DNA interaction associated with developmental changes in transcriptional activity. ...
Tendon, porous matrix of cross-linked collagen and glycosaminoglycan matrix (tenoglide tendon protector sheet), per square centimeter (Tenoglide tendon prot, cm2)
We recently synthesized and characterized water-soluble dinuclear Ru-II arene complexes, in which two {(eta(6)-p-isopropyltoluene)RuCl[3-(oxo-kappa O)-2-methyl-4-pyridinonato-kappa O4]} units were linked by flexible chains of different length [(CH2)(n) (n = 4, 6, 8, 12)]. These new dinuclear ruthenium drugs were found to exert promising cytotoxic effects in human cancer cells. In the present work DNA modifications by these new dinuclear Ru-II arene compounds, which differed in the length of the linker between the two Ru-II centers, were examined by biochemical and biophysical methods. The complexes bind DNA forming intrastrand and interstrand cross-links in one DNA molecule in the absence of proteins. An intriguing aspect of the DNA-binding mode of these dinuclear Ru-II compounds is that they can cross-link two DNA duplexes and also proteins to DNA-a feature not observed for other antitumor ruthenium complexes. Thus, the concept for the design of interhelical and DNA-protein cross-linking agents ...
Cisplatin is a crucial agent in the treatment of many solid tumors, yet many tumors have either acquired or intrinsic resistance to the drug. We have used the homozygous diploid deletion pool of Saccharomyces cerevisiae, containing 4728 strains with individual deletion of all nonessential genes, to systematically identify genes that when deleted confer sensitivity to the anticancer agents cisplatin, oxaliplatin, and mitomycin C. We found that deletions of genes involved in nucleotide excision repair, recombinational repair, postreplication repair including translesional synthesis, and DNA interstrand cross-link repair resulted in sensitivity to all three of the agents, although with some differences between the platinum drugs and mitomycin C in the spectrum of required translesional polymerases. Putative defective repair of oxidative damage (imp2Delta strain) also resulted in sensitivity to platinum and oxaliplatin, but not to mitomycin C. Surprisingly in light of their different profiles of ...
A novel photocrosslinkable polymeric system has been developed for processing into films having stable second-order nonlinear optical properties. In the present system, polymers bearing photo-crosslinkable chromophores, such as polyvinylcinnamate and are reacted with appropriately designed nonlinear optical molecules with the cinnamate or other photocrosslinkable functionalities for photocrosslinking at one, two or more points. The system can be poled and photocrosslinked in the poled state to yield a material with stable optical nonlinearity and large electro-optic coefficients.
April 2015 - San Diego (USA). At the World Cornea Congress the results of different keratoconus Cross-Linking technics have been discussed.. Cross-linking is a corneas treatment for patients with keratoconus. The main purpose of this treatment is improve the links between collagens molecules of the cornea. The treatment can be done with EPI-OFF technic (removing corneal epithelium) or with EPI-ON technic (corneal epithelium isnt removed).. At San Diego was show how the EPI-ON technic(transepithelial Cross-Linking) is less powerful than EPI-OFF (keratoconus Cross-Linking).. ...
COVID-19 (short for "Coronavirus Disease 2019) is the disease caused by the novel coronavirus, SARS-CoV-2. Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. COVID-19 represents a particular challenge to people with serious chronic medical conditions such as diabetes. To keep our community as informed as possible, the American Diabetes Association is partnering with the Chan Zuckerberg Initiatives Meta to highlight the latest research developments at the intersection of COVID-19 and diabetes. ...