Incubation of adriamycin resistant Chinese hamster lung cells with low levels of N-ethylmaleimide (NEM) results in a major increase in the cellular accumulation of drug. When resistant cells are prelabeled with [32Pi] and thereafter treated with NEM there also occurs a selective superphosphorylation of an 180K plasma membrane glycoprotein (P-180). This phosphorylation reaction occurs at both serine and threonine residues. In similar experiments with drug sensitive cells only minor levels of this protein can be detected. Detailed studies have established that in cells which have reverted to drug sensitivity there is a parallel loss in the presence of phosphorylated P-180. Also in cells which have undergone partial reversion to drug sensitivity there is a correlation between levels of superphosphorylated P-180 and adriamycin resistance. These results provide evidence that adriamycin resistance is dependent on the presence of P-180. The results also suggest that the biological activity of this protein is
Used for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. Corning™ cellgro™ DMEM/Hams Medium F-12 Mix is based on Hams F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. X6 500 mL Hams F-12 Medium w L-glut ...
N,N,N,N-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.
We have isolated three independent Chinese hamster ovary cell mutants (B3853, I223, and M311) with temperature-sensitive, pleiotropic defects in receptor-mediated endocytosis. Activities affected at 41 degrees C include uptake via the D-mannose 6-phosphate receptor, accumulation of Fe from diferric transferrin, uptake of alpha 2-macroglobulin, compartmentalization of newly synthesized acid hydrolases, resistance to ricin, and sensitivity to diphtheria and Pseudomonas toxins and modeccin. The three mutants also displayed decreased sialylation of some secreted glycoproteins at 41 degrees C, reminiscent of the nonconditional mutant DTG1-5-4 that showed both endocytic and Golgi-associated defects (Robbins, A.R., C. Oliver, J.L. Bateman, S.S. Krag, C.J. Galloway, and I. Mellman, 1984, J. Cell Biol., 99:1296-1308). Phenotypic changes were detectable within 30 min after transfer of the mutants to 41 degrees C; maximal alteration of most susceptible functions was obtained 4 h after temperature shift. At ...
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks. ...
The present study aimed to submit the transglutaminase (TGase) from a |I|Bacillus circulans|/I| strain isolated from the Amazon environment to |I|in vivo|/I| and |I|in vitro|/I| toxicological evaluations in order to assess its safety in food. The |I| in vivo|/I| assay was assessed using male Wistar rats in a subacute 14 day oral feeding test using a liquid enzyme preparation administered to 150 U kg b.wt. day|SUP|-1|/SUP|. The evaluation of cytotoxicity, genotoxicity and mutagenic effects of this microbial TGase was carried out using Chinese hamster lung fibroblasts cultured cells. No evidence of short term |I|in vivo|/I| toxicity was found for the enzymatic preparation in the subacute 14 day oral toxicity study using white Wistar rats models, daily treated with 150 U kg b.wt. day|SUP|-1 |/SUP|of TGase preparation. Furthermore, there were no statistical differences between the groups for relative weight gain and for hematological and clinical chemistry values. Histopathological examination of liver,
Metabolic cooperation assays were conducted with Chinese-hamster-V79 cells at three separate laboratories using standardized protocols and chemicals of known tumor promoting activity. Each laboratory used identical lots of test chemicals, solvents, serum, medium, and trypsin as well as a standard protocol. The chemicals to be tested were either known tumor promoters or had related chemical structu
Posted on small animals bit, but thought Id ask here. Im 14 weeks and as DP works away, Ive been cleaning out my DDa hamster cage all of this pre
BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
Substrate effects on the activation kinetics of Chinese hamster dihydrofolate reductase by p-chloromercuribenzoate (pCMB) have been studied. On the basis of the kinetic equation of substrate reaction in the presence of pCMB, all modification kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present study indicate that the modification of Chinese hamster dihydrofolate reductase by pCMB shows single-phase kinetics, and that changes in the enzyme activity and tertiary structure proceed simultaneously during the modification process. Both substrates, NADPH and 7,8-dihydrofolate, protect dihydrofolate reductase against modification by pCMB. In the presence of a saturating concentration of NADPH, the value of kcat for 7,8-dihydrofolate in the enzyme-catalysed reaction increased four-fold on modification of Cys-6, accompanied by a two-fold increase in Km for the modified enzyme. The utilization of the binding energy of a ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
BioAssay record AID 4623 submitted by ChEMBL: Displacement of [3H]5-HT from human 5-hydroxytryptamine 1D receptor expressed in Chinese hamster ovary cells (CHO cells).
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
I am trying to determine the best dose of hygromycin that i can use to select my transfected colonies. (working with chinese hamster cell lines). I have tried several concentrations (from 50-250 microgram per ml) on my host cells and changing the media plus antibiotic every 1-2 days. Each time the media has turned yellow and lots of dead cells but still surviving colonies can be seen. How could we know if the cell death is because of the overgrowth of the culture and consequent bad condition or because of the antibiotic?. ...
CGEN-15001T is a novel B7/CD28-like immune checkpoint target candidate discovered by Compugen.. Studies testing the immune function of CGEN-15001T, as a membrane bound protein or using a recombinant fusion protein containing the extracellular domain of CGEN-15001T, showed it is capable of inhibiting T cell activation, promoting a Th1 to Th2 shift, and potentially inducing immune tolerance. CGEN-15001T is expressed in subpopulations of immune cells, mainly macrophages, in both tumor and normal tissue samples.. In August 2013, Compugen signed a research and discovery collaboration and license agreement with Bayer HealthCare for the development and commercialization of antibody-based therapeutics for cancer immunotherapy against CGEN-15001T. After achieving all preclinical stage milestones, this program was transferred to Bayer for further development. To date, preclinical activities are on track, and pivotal toxicity studies and GMP clinical trial material production are ongoing.. ...
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
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Eukaryotic expression vectors have been used successfully in viral LT-expressing cell lines (ie. COS) to clone cDNAs encoding proteins that can be detected through their bio-activity or reactivity with specific antibodies. Since Chinese hamster ovary cells (CHO) have been used extensively for the is... DRIVER (Chinese) ...
Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
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Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 2 (XRCC2), mRNA. (H00007516-R02) - Products - Abnova
Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), mRNA. (H00007517-R02) - Products - Abnova
DIY (Do it yourself) Hamster Projects! - A place to share your creative hamster creations, from custom cage set-ups. home made bin cages, homemade toy
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. The Chinese hamster had been used in research since 1919 where they were used in place of mice for typing pneumococci. They were subsequently found to be excellent vectors for transmission of kala-azar (a.k.a. visceral leishmaniasis), facilitating leishmania research. In 1948, the Chinese hamster was first used in the United States for breeding in research laboratories. In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganians laboratory at the Boston Cancer Research Foundation and used it to ...
Mometasone furoate increased chromosomal aberrations in an in vitro Chinese hamster ovary-cell assay, but did not increase chromosomal aberrations in an in vitro Chinese hamster lung cell assay. Mometasone furoate was not mutagenic in the Ames
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., "Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells." (1981). Subject Strain Bibliography 1981. 19 ...
Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy.
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. Biotechnol Bioeng. 2019 Aug 12 ...
BGI, the giant genomics institute located in Shenzhen, and GT Life Sciences of San Diego have published their collaborative study on the genomic sequence of the Chinese hamster ovary (CHO) K1 cell line in Nature Biotechnology. Over 70% of the recombinant therapeutic proteins sold today are manufactured using mammalian cells, primarily CHO cell lines. GT Life Sciences uses a metabolic modeling platform to design new products and processes for the life sciences field. It says a better understanding of the genome will speed development of new recombinant protein therapies. More details.... Share this with colleagues:   var switchTo5x=true;stLight.options({publisher:d7871f5b-67bc-4d30-b66f-1465d0b97213});
Compare X-ray repair complementing defective repair in Chinese hamster cells 6 Biomolecules from Aviva Systems Biology from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
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Sundaram, H., Strange, Philip G. (1994) Characterisation of the human brain serotonin 5-HT1A receptor expressed in Chinese Hamster Ovary cells. Biochemical Society Transactions, 22 (1). S75-S75. ISSN 0300-5127. (doi:10.1042/bst022075s) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
A blog by a hamster fanatic written for other hamster fanatics. Journeying my researching, owning, showing and breeding of hamsters.
A blog by a hamster fanatic written for other hamster fanatics. Journeying my researching, owning, showing and breeding of hamsters.
The Cantering Chef has baked up something unique for your horse! His tasty horse cookies combine the freshest natural ingredients, including scrumptious rolled oats and molasses. C...
产品:invitrogen货号:K1483规格:2×10^6cells名称:GeneBLAzer®MC2R-CRE-bla-CHO-K1CellsTheGeneBLAzer®MC2R-CRE-bla-CHO-K1cellscontainthehumanmelanocortin-2receptor(MC2R),(Accession#NM_000529.1)andtheMe
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ഒരു അപൂരിത ആലിഫാറ്റിക ആൽഡിഹൈഡ്. ഫോർമുല, CH2 = CH - CHO. നിറമില്ലാത്ത ദ്രവവസ്തു. തിളനില 53oC. അസഹ്യമായ ഗന്ധമുണ്ട്. ജലത്തിൽ അലിയും. വെറുതെ വച്ചിരുന്നാൽതന്നെ പോളിമറീകരിച്ചു വെളുത്ത പൊടിയായി മാറുന്നു. അക്രൊലീൻ ആൽഡിഹൈഡിന്റെയും ഒലിഫീനിന്റെയും രാസഗുണധർമങ്ങൾ പ്രദർശിപ്പിക്കുന്നു. അക്രൊലീന്റെ നിരോക്സീകരണംവഴി പല യൗഗികങ്ങളും ഉത്പാദിപ്പിക്കാം. മഗ്നീഷ്യം അമാൽഗം, സോഡിയം അമാൽഗം, അലൂമിനിയം ഐസൊ ...
About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
TY - JOUR. T1 - Natural Estrogens Induce Modulation of Microtubules in Chinese Hamster V79 Cells in Culture. AU - Aizu-Yokota, Eriko. AU - Susaki, Akiko. AU - Sato, Yoshihiro. PY - 1995/5/1. Y1 - 1995/5/1. N2 - Natural estrogens and their derivatives comprising 30 compounds in total were tested for their ability to induce microtubule disruption in Chinese hamster V79 cells. The cytoplasmic microtubule networks were examined by the indirect immunofluorescence method using anti-β-tubu-lin antibody. The effective concentrations of 17β-estradfol (Ej-Hβ) and 17α-estradiol required for the induction of microtubule distribution in 50% of the cells (EC50) in 1 h were 10 and 9 µm for V79 cells, respectively, and 2-methoxyestradiol showed the strongest activity (ECso 2 µm) among the tested compounds including the catechol estrogens 2-hydroxyestradiol, 4-hydroxyestradiol, 2-hydroxyestrone, and 4-hydroxyestrone. The estrone series of estrogens showed relatively low activity for disruption of ...
Pentachlorophenol (91.6% pure) was tested in Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 at doses up to 30 µg/plate with and without induced rat or hamster liver S9; no significant increases in the number of revertant colonies were observed in any of the strain/activation combinations. When tested for cytogenetic effects in cultured Chinese hamster ovary cells, pentachlorophenol was weakly positive for induction of sister chromatid exchanges and chromosomal aberrations. In the sister chromatid exchange test, a weakly positive response was observed within a concentration range of 3 to 30 µg/mL in the absence of S9; with S9, no induction of sister chromatid exchanges was noted. In the chromosomal aberrations test, pentachlorophenol was negative without S9 but induced small but significant increases in the frequency of aberrant cells in the presence of S9 at doses of 80 and 100 µg/mL. In contrast to the positive in vitro results in the test for induction of chromosomal ...
Cyclohexanone oxime is used primarily as a captive intermediate in the synthesis of caprolactam for the production of polycaprolactam (Nylon-6) fibers and plastics and also in a variety of industrial applications. Cyclohexanone oxime was selected for study because of the potential for human exposure and the interest in oximes as a chemical class. Toxicity studies of cyclohexanone oxime (approximately 99% pure) were carried out in male and female B6C3F1 mice; the compound was administered in drinking water for 2 weeks or 13 weeks. In addition, the genetic toxicity of cyclohexanone oxime was evaluated by determining mutagenicity in Salmonella typhimurium and induction of chromosomal aberrations in cultured Chinese hamster ovary cells in vitro, with and without S9 activation. The frequency of micronucleated normochromatic erythrocytes in the bone marrow and peripheral blood of mice from the 13-week study was also determined.. In the 2-week study, groups of five male and five female mice were given ...
The kinetic theory of substrate reaction during modification of enzyme activity has been applied to the study of inactivation kinetics of Chinese hamster dihydrofolate reductase by urea [Tsou (1988) Adv. Enzymol. Relat. Areas Mol. Biol. 61, 381-436]. On the basis of the kinetic equation of substrate reaction in the presence of urea, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present study indicate that the denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process. Both substrates, NADPH and 7,8-dihydrofolate, protect dihydrofolate reductase against inactivation, and enzyme-substrate complexes lose their activity less rapidly than the free enzyme.. ...