About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
N,N,N,N-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.
We have isolated three independent Chinese hamster ovary cell mutants (B3853, I223, and M311) with temperature-sensitive, pleiotropic defects in receptor-mediated endocytosis. Activities affected at 41 degrees C include uptake via the D-mannose 6-phosphate receptor, accumulation of Fe from diferric transferrin, uptake of alpha 2-macroglobulin, compartmentalization of newly synthesized acid hydrolases, resistance to ricin, and sensitivity to diphtheria and Pseudomonas toxins and modeccin. The three mutants also displayed decreased sialylation of some secreted glycoproteins at 41 degrees C, reminiscent of the nonconditional mutant DTG1-5-4 that showed both endocytic and Golgi-associated defects (Robbins, A.R., C. Oliver, J.L. Bateman, S.S. Krag, C.J. Galloway, and I. Mellman, 1984, J. Cell Biol., 99:1296-1308). Phenotypic changes were detectable within 30 min after transfer of the mutants to 41 degrees C; maximal alteration of most susceptible functions was obtained 4 h after temperature shift. At ...
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks. ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells. (1981). Subject Strain Bibliography 1981. 19 ...
BioAssay record AID 51937 submitted by ChEMBL: K+ channel blocking activity in Chinese hamster ovary cells expressing HERG Kv11.1.
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
The technology is set to heighten the companys GPEx expression platform through the utilization of a glutamine synthase knock-out Chinese hamster ovary cell line.
Synbio Technologies provides a variety of reporter expression vectors to meet the screening needs of different cell lines, and easily detect the transfection efficiency and expression level in cells.
Eukaryotic expression vectors have been used successfully in viral LT-expressing cell lines (ie. COS) to clone cDNAs encoding proteins that can be detected through their bio-activity or reactivity with specific antibodies. Since Chinese hamster ovary cells (CHO) have been used extensively for the is... DRIVER (Chinese) ...
Is there anybody who get a stabile transformation with the pcDNA3 vector (INVITROGEN) in BHK21 (baby hamster kidney) cells and what was the level of overexpression compared to a normal cell line expressing the inserted cDNA? Thanks Dietmar ...
The purpose of this study is to assess the safety, pharmacokinetic and activity profiles of the ch14.18 antibody produced in cells of hamster origin (ch
mutant moments e.p. (2020 reissue) by soft cell buy online at resident, to celebrate 40 years since the release of their first ever recording, soft
BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
TY - JOUR. T1 - Dietary D-allulose alters cholesterol metabolism in Golden Syrian hamsters partly by reducing serum PCSK9 levels. AU - Kanasaki, Akane. AU - Jiang, Zhe. AU - Mizokami, Takuya. AU - Shirouchi, Bungo. AU - Iida, Tetsuo. AU - Nagata, Yasuo. AU - Sato, Masao. PY - 2019/9/1. Y1 - 2019/9/1. N2 - D-Allulose, a C-3 epimer of D-fructose, is a rare sugar reported to be a non-caloric sweetener having several health beneficial effects including anti-hyperglycemia and anti-obesity. However, the impact of dietary D-allulose on cholesterol metabolism remains unclear. Therefore, we studied the effects of D-allulose on the cholesterol metabolism of Golden Syrian hamsters, an animal model with a lipid metabolism similar to that of humans. Hamsters received either normal diet (ND) or high-fat diet (HFD) with or without 3% D-allulose for 4 or 8 weeks. While there were no significant differences in total serum cholesterol levels between the groups, D-allulose significantly increased HDL-cholesterol ...
Monoclonal antibodies specific for epitopes on the perstussigen molecule, s-1, were developed by the Fetuin-ELISA test, radioimmunoassays and Western Blott(27). Hybridoma fluids already established for specificity on s-2, s-4 and the undigested molecule were exposed to pertussigen to detect the neutralization of agglutination to Chinese Hamster Ovary (CHO) cells. The results showed that no appreciable neutralization occurred. Many more monoclonals need to be developed and tested to find the critical epitope or epitopes which cause agglutination to CHO cells. This research may provide a clue to the specific antigenic site which causes toxicity attributed to whooping cough in humans ...
Phodopus campbelli is very small in size and the pelage is short and silky. The underside of the animal is covered in soft, buff, light grey fur and the dorsal portions, including the head, are woody brown in color. The underfur is quite short and is a dark slate grey. A defined charcoal stripe runs from between the ears to the tail. The pads of all digits, and the small tail, are covered in silky white fur. Additionally, Campbells hamsters, like other members of the subfamily Cricetinae, possess large internal cheek pouches that terminate above the scapula. Males are larger than females. (Thomas, 1905). Phodopus campbelli is often confused with Phodopus sungorus, Siberian hamsters. However, there are several physical characteristics that distinguish the two species. The ears of Phodopus campbelli are generally smaller than those of Phodopus sungorus. The mid-dorsal stripe of the Campbells hamster is both thin and defined and the area where the dorsal fur meets the ventral fur is a creamy ...
TY - JOUR. T1 - Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. AU - Turley, E. AU - Armstrong, NC. AU - Wallace, JMW. AU - Gilmore, WS. AU - McKelvey-Martin, VJ. AU - Allen, J. AU - Strain, JJ. PY - 1999/1. Y1 - 1999/1. N2 - Cholesterol oxides are cytotoxic and have been implicated in many disease processes; however, it has been proposed that cholesterol oxides result from cholesterol acting as a sacrificial antioxidant. In this study, the effect of dietary cholesterol on DNA damage, assessed by the alkaline comet assay, was examined in male and female Syrian hamsters. Animals were fed ad libitum a modified AIN-76 diet (control) or a diet with 0.5% cholesterol for 10 weeks. Following the 10-week feeding period, there was no significant difference in body weight between cholesterol-fed and control animals. Cholesterol feeding resulted in significant liver hypertrophy, and increased plasma total and HDL cholesterol in both male and female animals compared with ...
The role of polyamines in macromolecular synthesis has been studied using the synthesis of Semliki-Forest virus (SF virus) in normal and alpha-difluoromethylornithine-treated baby-hamster kidney (BHK21) cells as a model system. The activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase, the rate-limiting enzymes in polyamine biosynthesis, decreased rapidly in mock- and SF-virus-infected cells, indicating that virus production in BHK21 cells was not dependent on polyamines formed after infection. A prolonged treatment of BHK21 cells with alpha-difluoro-methylornithine, a specific inhibitor of polyamine synthesis, resulted in a marked inhibition of the initial rate of virus production, which appeared 72 h after the beginning of the treatment. This inhibition was reversed by putrescine, spermidine and spermine, and at last partially by several other diamines and polyamine homologues. Polyamine-depletion also markedly reduced viral RNA polymerase activity in SF-virus infected ...
TY - JOUR. T1 - Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. AU - Thompson, L. H.. AU - Busch, D. B.. AU - Brookman, K.. AU - Mooney, C. L.. AU - Glaser, D. A.. PY - 1981. Y1 - 1981. N2 - Mutant lines of Chinese hamster ovary cells that show hypersensitivity to killing and mutagenesis by UV light were analyzed by genetic complementation analysis to determine whether defects in different gene loci might underlie a common cellular phenotype. To facilitate rapid screening of mutant clones, a procedure was devised that allowed presumptive complementation to be assessed on the basis of the frequency of UV-resistant cells after fusion by polyethylene glycol. Four classes were identified among 44 clones tested. By using drug-resistance markers for selection of hybrid cells in crosses between UV mutant and wild type, a mutant from each of the four classes was shown to behave as phenotypically recessive. Hybrids were also isolated from crosses between each of the ...
Human apolipoprotein (apo) A-I is secreted as a proprotein of 249 amino acids and is processed extracellularly to the mature form (243 amino acids) by removal of a six-residue propeptide segment. We have examined the role of the apoA-I propeptide in intracellular transport and secretion using transfected baby hamster kidney cells that secreted either proapoA-I (from the wild-type cDNA, A-Iwt) or mature-form apoA-I (from A-I delta pro, a cDNA in which the propeptide sequence was deleted). Deletion of the propeptide from the apoA-I sequence did not affect the rate of apoA-I synthesis, nor did it affect the fidelity of proteolytic removal of the prepeptide. However, the propeptide deletion caused mature-form apoA-I to accumulate within the cells as determined by pulse-chase experiments; the intracellular retention times for the mature-form apoA-I in which the propeptide was prematurely removed was three times longer than that of proapoA-I (t1/2 , 3 h compared with approximately 50 min). There was ...
Goat anti-Golden Syrian hamster IgG recognizes Golden Syrian hamster IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Rhodamine. Golden Syrian hamster IgG whole molecule (PAB10592) - Products - Abnova
Rabbit anti-Golden Syrian hamster IgG recognizes Golden Syrian hamster IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. Golden Syrian hamster IgG whole molecule (PAB9233) - Products - Abnova
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
TY - JOUR. T1 - One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells. AU - Yang, Ganglong. AU - Höti, Naseruddin. AU - Chen, Shao Yung. AU - Zhou, Yangying. AU - Wang, Qiong. AU - Betenbaugh, Michael. AU - Zhang, Hui. PY - 2020/4/17. Y1 - 2020/4/17. N2 - Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. Moreover, an increased ...
TY - CONF. T1 - Engineering amino acid supply pathways in Chinese hamster ovary cells. AU - Ley, Daniel. AU - Lund, Anne Mathilde. AU - Rank, Julie. AU - Kildegaard, Helene Faustrup. AU - Andersen, Mikael Rørdam. N1 - Conference code: 9. PY - 2014. Y1 - 2014. M3 - Poster. T2 - 9th Danish Conference on Biotechnology and Molecular Biology. Y2 - 22 May 2014 through 23 May 2014. ER - ...
Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy.
Download Caffeine-induced alterations in non-histone chromosomal proteins of Chinese hamster ovary cells ebook by Susan Claire HarrisonType: pdf, ePub, zip, txt
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
Drugs and materials. All drugs and chemicals were purchased from Sigma Chemical Co. (St. Louis, MO). Except for fetal bovine serum (FBS) that was from Gemini Bio-Products (Woodland, CA), media and reagents for tissue culture work and transfection were purchased from Life Technologies/Bethesda Research Laboratories (Grand Island, NY). DNase-free RNase and propidium iodide were purchased from Roche Molecular Biochemicals (Indianapolis, IN).. Cell cultures. The Chinese hamster ovary (CHO) xrs-6 and hamster Ku86 cDNA-complemented xrs-6+hamKu86 cells ( 14) were purchased from the European Collection of Cell Cultures (Wiltshire, United Kingdom). The Chinese hamster lung fibroblast XR-V15B cells ( 15) were purchased from the American Type Culture Collection (Manassas, VA). All cell lines were grown in HAMs F12 medium supplemented with 10% FBS, glutamate, penicillin, and streptomycin, in a humidified 37°C incubator at 5% CO2. The media for the xrs-6+hamKu86 and the four XR-V15B-transfectant sublines ...
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. Biotechnol Bioeng. 2019 Aug 12 ...
The parent line of BHK-21(C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation.
The parent line of BHK-21(C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation.
Used for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. Corning™ cellgro™ DMEM/Hams Medium F-12 Mix is based on Hams F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. X6 500 mL Hams F-12 Medium w L-glut ...
Phenotypic variations in the cells arise from changes in their DNA, including differences in methylation patterns. From analyzing CHO cell lines, researchers found that the transcriptome of each subclone also had a significant number of individual changes and that such changes indicate that epigenetic regulation is a hidden but important player in cell line development.
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Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
We constructed comparative cytogenetic maps of the Chinese hamster to mouse, rat and human by fluorescence in-situ hybridization using 36 cDNA clones of mouse, rat, Syrian hamster, Chinese hamster and human functional genes. In this study, 30 out of the 36 genes were newly mapped to Chinese hamster …
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
I am trying to determine the best dose of hygromycin that i can use to select my transfected colonies. (working with chinese hamster cell lines). I have tried several concentrations (from 50-250 microgram per ml) on my host cells and changing the media plus antibiotic every 1-2 days. Each time the media has turned yellow and lots of dead cells but still surviving colonies can be seen. How could we know if the cell death is because of the overgrowth of the culture and consequent bad condition or because of the antibiotic?. ...
First off, a VERY IMPORTANT NOTE. Mulder is a Roborovski hamster, which is the smallest of the dwarf hamsters. When Scully died, he weighed 22g, or 0.78oz; robos are usually less than 30g, or 1.06oz. Other dwarf hamsters are slightly larger, and can weigh up to 2.5oz. Syrian hamsters, on the other hand, usually weigh 5-7oz. This house is a little less than 450 square inches, which is a decent size for a dwarf hamster, but would be entirely too small for a Syrian hamster. If you need a house for a Syrian hamster, I highly recommend that you consider adapting these instructions for a much larger table, such as the Lack coffee table or two or more connected Lack side tables ...
SWISS-MODEL Repository entry for P86206 (CH60_MESAU), 60 kDa heat shock protein, mitochondrial. Mesocricetus auratus (Golden hamster)
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A research team at the Northeast Agricultural University in Harbin managed to breed three transgenic pigs by injecting fluorescent green protein and a bunch of other junk into embryonic pigs, said Professor Liu Zhonghua. Liu wore a fancy white lab coat and had multiple degrees adorning his walls, so we assume he must be pretty smart ...
Mammalian Cell Culture Fast Track Project commissioned, loop tuned, validated for initial startup Wrote and executed all FAT, validation protocols on site.
Syrian hamsters make for wonderful home pets if handled carefully. Their playful, cranky, and quirky personality attracts many pet owners. They demand little
Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
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Shop a large selection of products and learn more about CD49b Hamster anti-Mouse, BB700, Clone: HMα2, BD Optibuild 50μg; BB700 50μg; BB700.
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Sundaram, H., Strange, Philip G. (1994) Characterisation of the human brain serotonin 5-HT1A receptor expressed in Chinese Hamster Ovary cells. Biochemical Society Transactions, 22 (1). S75-S75. ISSN 0300-5127. (doi:10.1042/bst022075s) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
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