Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. The test was performed with unconcentrated human sera and the lines stained in an aqueous solution of Ponceau red. Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. There was no cross-reactivity with sera of patients with autoimmune disorders by either test.
The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.. ...
The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control subjects who did not have typhoid. Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. By Widal test the rate of detection in patients sera was 73% and the false-positive rate for control sera 16%. It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. An additional advantage of counterimmunoelectrophoresis is that results ...
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
To ascertain the heterogeneity of anti-prothrombin antibodies (aPT), we compared three in-house aPT ELISAs: A) medium binding plates, phosphatidylserine, prothrombin, Tris-buffered saline, calcium; B) high binding plates, prothrombin, Tris-buffered saline; and C) high binding plates, prothrombin, PBS. One serum, exhibiting high positive aPT in all three ELISAs, was selected as the calibrator. Sera from 47 patients (41 with SLE, 4 with pAPS, 2 with arterial thromboses) were tested for IgG and IgM aPT.. The results showed six different patterns: 1) similar results were obtained with A, B and C (similar analytical sensitivity); 2) similar results were obtained with A and B while C showed lower analitycal sensitivity; 3) B and C seemed analytically less sensitive than A; 4) A and B were analytically less sensitive than C; 5) A showed very low analytical sensitivity; 6) A and C showed lower analytical sensitivity than B.. The analysis of all the presented patterns showed noncomparable results of the ...
Anti-Prothrombin antibody conjugated to Biotin validated for WB, IP, RIA, cELISA and tested in Human. Immunogen corresponding to full length native protein…
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Approximate amount. All fees are tentative and subject to change. The application fee is nonrefundable. Tuition fees generally increase starting in August. Click here to download a PDF of the CIEP Dates & Fees. ...
Preparing for the CCIE Routing and Switching lab exam typically involves massive amounts of studying. But if you already possess Ciscos CCNP Routing and Switching certification, you already know much of what youll need to succeed on CCIEs labs. This book will help you bridge the gaps in your knowledge quickly and efficiently, and make the most of everything you already know.
Please note: CIE helpdesk is intended to give assistance regarding questions related to CIE publications or the technical work in CIE Technical Committees.. It is not intended to give advice or even provide technical solutions to questions such as what CIE publication to use or queries relating to the interpretation of CIE publications. We hope for your understanding that we will not reply to inquiries of that kind. Please check the CIE website regularly to stay informed about conferences, symposia or trainings for further information and training.. ...
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CIE has formal arrangements with several international and regional organizations, these arrangments are supported by liaison at the administrative level of these orgainzations. CIE also has working liaisons with both these and other orgainzaitons at the technical level, these working liaisons can be found within the Division pages of this website.. ...
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Notes for the CIE O Level Chemistry module: 2. The particulate nature of matter. These have been made according to the specifications 5070 (2017-2019) and cover all the relevant topics for examination in May/June.. CIE O Level 2. The particulate nature of matter Notes:. ...
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The SET-RPLA, from Denka Seiken Co. Ltd., Tokio, a commercial reversed passive latex agglutination test kit, has been recommended to establish the enterotoxicity capacity of some staphylococcal strains, implicated in food poisoning outbreaks that produce low levels of enterotoxins (SE). Despite the RPLA specificity, the occurrence of nonspecific reactions when testing low- SE-producing is common. In order to control these nonspecific reactions the addition of purified normal rabbit IgG purified was applied on approximately 350 staphylococcal isolates from human milk and anatomic sites of healthy dental student carriers. The results indicated that addition of 5% (v/v) of purified normal rabbit IgG (0.74 mg/mL) to the culture supernatant fluid is a simple and reliable tool for the controlling of nonspecific reactions in the RPLA assay ...
In a series of 163 ears with chronic, mucoid effusion, free or immune complex bound pneumococcal capsular polysaccharide antigens were demonstrated with Omniserum and counterimmunoelectrophoresis (CIEP) in 37 ears (23%). In crushed adenoid tissue thi
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody ...
TY - JOUR. T1 - La sierologia del Lupus eritematoso. AU - Parodi, A.. AU - Cozzani, E.. AU - Drosera, M.. PY - 2004. Y1 - 2004. N2 - Lupus erythematosus (LE), expecially its systemic form (SLE), is serologically characterized by the presence of autoantibodies directed to nuclear, cytoplasmic and cellular membrane antigens. Some of them are specific of the disease, others characterize only particular substes. Antinuclear antibodies (ANA) can be detected by various assays: indirect immunofluorescence (IIF), immunodiffusion (ID), counterimmunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and immunoblotting (IB). IIF and ELISA are the most used. ELISA is very sensitive, IIF is sensitive, easy to perform and cheap. In IIF monolayers of cultured cells particularly Hep-2 are the best substrate to use. Antibodies to ds-DNA are high specific of SLE and can be used to monitor the clinical course of the disease. Anti-nucleosome antibodies are now considered the ...
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Group members: Dr. Frida Piper, Dr. Alex Fajardo. Within the line of research, Terrestrial Ecosystems of the CIEP, diverse analyses are done associated with the research projects. The processing and analysis of vegetative matter demands effort and time. The process, from the moment the plants are gathered in the field to when they are converted into data with statistical value, requires a high investment in specialized human capital. For this reason, and because these are contextualized in projects of scientific research, the vegetal analyses done at the CIEP provide an opportunity to develop theses for masters degrees and PhDs. The aforementioned analyses are not offered as services.. Entry and processing of vegetative material. The plants or vegetal tissues are generally collected in the field, rarely coming from controlled conditions (nurseries, greenhouses). The first step consists of drying the material, then weighing it, and finally grinding it. For this purpose, the laboratory has a stove ...
Developments in spectroscopic analysis and routine availability of X-ray crystallography have simplified structural elucidation of natural compounds. However, isolating bioactive components of interest from crude extracts of either plants or animals has always been challenging for two reasons: these extracts contain hundreds of compounds with a range of polarities, and the bioactivity of the compound of interest should be maintained throughout the purification process. This is where countercurrent chromatography (CCC) is helpful.
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Agentúra ECHA podniká kroky na zlepšenie kvality informácií v registračných dokumentáciách s cieľom splniť svoj prvý strategický cieľ, ktorým je „maximalizovať dostupnosť vysokokvalitných údajov na umožnenie bezpečnej výroby a používania chemických látok". V rámci týchto krokov agentúra ECHA využíva skúsenosti získané z hodnotenia registračných dokumentácií a časté nedostatky premieta do počítačových algoritmov, ktoré sa môžu použiť na kontrolu celej databázy. To umožňuje agentúre získať lepší prehľad o kvalite registračných údajov a pomáha jej účinnejšie riešiť zistené nezrovnalosti.. Výsledky pôvodných kontrol sa oznamujú registrujúcim prostredníctvom neformálnych listov, ktoré takisto obsahujú informácie o tom, ako odstrániť možné nezrovnalosti. Agentúra očakáva, že spoločnosti budú brať tieto listy vážne a spontánne aktualizovať svoje registrácie, pričom vyriešia zdôraznené výhrady a tieto ...
This report is a revision and update of CIE 150:2003 Guide on the Limitation of the Effects of Obtrusive Light from Outdoor Lighting Installations. The purpose of this Guide is to help formulate guidelines for assessing the... more ...