Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. The test was performed with unconcentrated human sera and the lines stained in an aqueous solution of Ponceau red. Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. There was no cross-reactivity with sera of patients with autoimmune disorders by either test.

The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.. ...

The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control subjects who did not have typhoid. Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. By Widal test the rate of detection in patients sera was 73% and the false-positive rate for control sera 16%. It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. An additional advantage of counterimmunoelectrophoresis is that results ...

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...

To ascertain the heterogeneity of anti-prothrombin antibodies (aPT), we compared three in-house aPT ELISAs: A) medium binding plates, phosphatidylserine, prothrombin, Tris-buffered saline, calcium; B) high binding plates, prothrombin, Tris-buffered saline; and C) high binding plates, prothrombin, PBS. One serum, exhibiting high positive aPT in all three ELISAs, was selected as the calibrator. Sera from 47 patients (41 with SLE, 4 with pAPS, 2 with arterial thromboses) were tested for IgG and IgM aPT.. The results showed six different patterns: 1) similar results were obtained with A, B and C (similar analytical sensitivity); 2) similar results were obtained with A and B while C showed lower analitycal sensitivity; 3) B and C seemed analytically less sensitive than A; 4) A and B were analytically less sensitive than C; 5) A showed very low analytical sensitivity; 6) A and C showed lower analytical sensitivity than B.. The analysis of all the presented patterns showed noncomparable results of the ...

Anti-Prothrombin antibody conjugated to Biotin validated for WB, IP, RIA, cELISA and tested in Human. Immunogen corresponding to full length native protein…

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Approximate amount. All fees are tentative and subject to change. The application fee is nonrefundable. Tuition fees generally increase starting in August. Click here to download a PDF of the CIEP Dates & Fees. ...

Preparing for the CCIE Routing and Switching lab exam typically involves massive amounts of studying. But if you already possess Ciscos CCNP Routing and Switching certification, you already know much of what youll need to succeed on CCIEs labs. This book will help you bridge the gaps in your knowledge quickly and efficiently, and make the most of everything you already know.

CCIE Service Provider v4 boot camp (Online) is a 10-day workshop exploring skills required of a Network Engineer to design, configure, optimize, diagnose, and troubleshoot a complex highly available Service Provider network infrastructure. Candidates preparing for CCIE Service Provider certification possess thorough knowledge in IPv4 and IPv6 core, aggregation/edge, remote access technologies, Layer 2 and Layer 3 VPNs, plus managed services traversing an IP core networks. They also have an overall knowledge in security, virtualization and mobility technologies.. ...

About 6 months ago I finally passed the CCIE. I decided after that to take a break for a bit. Then maybe do a post to write up how I passed the CCIE - because I had changed so much between the 3rd and 4th attempt

Please note: CIE helpdesk is intended to give assistance regarding questions related to CIE publications or the technical work in CIE Technical Committees.. It is not intended to give advice or even provide technical solutions to questions such as what CIE publication to use or queries relating to the interpretation of CIE publications. We hope for your understanding that we will not reply to inquiries of that kind. Please check the CIE website regularly to stay informed about conferences, symposia or trainings for further information and training.. ...

Theres a new story being told in the networking industry. The CCIE isnt what it used to be, and pursuing it doesnt make as much sense as in years past. My initial response to this is simple: BALONEY. My thoughts arent based on a formal research study, so please keep that in mind. Over the…

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CIE has formal arrangements with several international and regional organizations, these arrangments are supported by liaison at the administrative level of these orgainzations. CIE also has working liaisons with both these and other orgainzaitons at the technical level, these working liaisons can be found within the Division pages of this website.. ...

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Notes for the CIE O Level Chemistry module: 2. The particulate nature of matter. These have been made according to the specifications 5070 (2017-2019) and cover all the relevant topics for examination in May/June.. CIE O Level 2. The particulate nature of matter Notes:. ...

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The SET-RPLA, from Denka Seiken Co. Ltd., Tokio, a commercial reversed passive latex agglutination test kit, has been recommended to establish the enterotoxicity capacity of some staphylococcal strains, implicated in food poisoning outbreaks that produce low levels of enterotoxins (SE). Despite the RPLA specificity, the occurrence of nonspecific reactions when testing low- SE-producing is common. In order to control these nonspecific reactions the addition of purified normal rabbit IgG purified was applied on approximately 350 staphylococcal isolates from human milk and anatomic sites of healthy dental student carriers. The results indicated that addition of 5% (v/v) of purified normal rabbit IgG (0.74 mg/mL) to the culture supernatant fluid is a simple and reliable tool for the controlling of nonspecific reactions in the RPLA assay ...

In a series of 163 ears with chronic, mucoid effusion, free or immune complex bound pneumococcal capsular polysaccharide antigens were demonstrated with Omniserum and counterimmunoelectrophoresis (CIEP) in 37 ears (23%). In crushed adenoid tissue thi

Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody ...

Bruder RC et al. The importance of counterimmunoelectrophoresis in the detection of extractable nuclear antigens for the diagnosis of systemic rheumatic diseases. J. Bras. Patol. Med. Lab. vol.40 no.1 Rio de Janeiro Feb. 2004. Autoimmune Manifestations in Acute Viral Hepatitides ...

TY - JOUR. T1 - La sierologia del Lupus eritematoso. AU - Parodi, A.. AU - Cozzani, E.. AU - Drosera, M.. PY - 2004. Y1 - 2004. N2 - Lupus erythematosus (LE), expecially its systemic form (SLE), is serologically characterized by the presence of autoantibodies directed to nuclear, cytoplasmic and cellular membrane antigens. Some of them are specific of the disease, others characterize only particular substes. Antinuclear antibodies (ANA) can be detected by various assays: indirect immunofluorescence (IIF), immunodiffusion (ID), counterimmunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and immunoblotting (IB). IIF and ELISA are the most used. ELISA is very sensitive, IIF is sensitive, easy to perform and cheap. In IIF monolayers of cultured cells particularly Hep-2 are the best substrate to use. Antibodies to ds-DNA are high specific of SLE and can be used to monitor the clinical course of the disease. Anti-nucleosome antibodies are now considered the ...

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Prednášky pre 6.ročník „Zubné lekárstvo zimný semester ak. Rok 2016/2017 Prednášky: 1. - 7., 9. - 13. týždeň „Maxilofaciálna chirurgia, poslucháreň Heydukova (utorok 16.30 - 18.20 hod.) 1. Dentoalveolárna chirurgia - opakovanie (anatómia dutiny ústnej, lokálna anestézia, indikácie, komplikácie lokálne, celkové, Extrakcie zubov, indikácie, technika, komplikácie lokálne, endodonticko- chirurgické ošetrenie devitálnych zubov, indikácie, technika, rtg) 2. Dentoalveolárna chirurgia - opakovanie (tretie moláre, vyšetrenie, indikácie extrakcie, asymptomatický tretí molár, technika extrakcie, chirurgická extrakcia, komplikácie, pooperačná starostlivosť) 3. Dentálna implantológia (princípy, základné typy dentálnych implantátov, chirurgická technika, indikácie, protetická sanácia defektov na dentálnych implantátoch) 4. Pacient na antiagregačnej a antikoagulačnej liečbe ( princípy a typy antiagregačnej a antikoagulačnej liečby, ...

Táto práca preukázala, že pravidelná fyzická aktivita strednej intenzity spôsobuje významné zmeny lipoproteínového profilu už v priebehu prvých 14 dní. Priaznivý efekt cvičenia na lipidový metabolizmus bol zdokumentovaný mnohými prácami. Tieto sa však líšili významne dlhším trvaním tréningového protokolu (2 mesiace až 1 rok) [10- 13,15]. Sledovanie zmien v takto krátkom období doposiaľ nebolo v tomto rozsahu v nami dostupnej literatúre realizované. Výrazné zmeny sme pozorovali v oblasti lipoproteínov bohatých na triacylglycerol (VLDL, IDL). Naša práca preukázala štatisticky významné zníženie koncentrácie VLDL v priebehu 14 dní. V literatúre je opísané zníženie koncentrácie VLDL a triacylglycerolu (TAG) pôsobením tréningového programu s využitím aeróbneho cvičenia strednej intenzity o trvaní 6 mesiacov [11,13,15]. K najmarkantnejšiemu zníženiu koncentrácie VLDL dochádza podľa literatúry u jedincov s ...

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Anyhow a couple of months ago my CCIE study came to an end not because I passed the lab unfortunatly but because the kids were to much of a handful for my wife to handle on her own on weekends. I do hope that not everything has fallen out of my head and that I can pick it up again sometime in the near future maybe in winter where I can trick my kids into believing it is bed time at 3pm in the afternoon :-) It will not be any time soon though because my lab is in pieces as I have decided to move house. Not only have I decided to move into a bigger more expensive house when interest rates are at their lowest levels in hundreds of years but I have also decided to move jobs and take a pay cut at the same time. Nothing like making life difficult for yourself hey? As you can see I am going stuggle with that CCIE as I am not the brightest lamp on the street ...

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Klinické skúšania majú za cieľ zistiť účinnosť a bezpečnosť skúšaného lieku, alebo liekovej kombinácie, či iného liečebného postupu. Pre účasť sú dopredu stanovené presné kritériá, ktoré musí pacient spĺňať, aby mohol byť v rámci klinického skúšania liečený.. Register onkologických skúšaní na Slovensku bol založený 15. januára 2019 s cieľom poskytovať informácie lekárom, ktorí liečia onkologických pacientov. Pri vzniku registra boli do neho zapísané všetky onkologické klinické skúšania, v rámci ktorých je na Slovensku otvorený nábor pre pacientov. Následne budú informácie v registri priebežne aktualizované podľa dostupných informácii zo Štátneho ústavu pre kontrolu liečiv (SÚKL), aby bolo zrejmé, ktoré klinické skúšania sú už pre nábor pacientov ukončené, alebo dočasne pozastavené, a ktoré sú a boli pre nábor pacientov otvorené.. V registri je tiež uvedený webová stránka clinicatrialsregister.eu so ...