To infect and replicate, Pseudoalteromonas virus PM2 delivers its genome across the cell envelope of two known marine host strains: gram-negative Pseudoalteromonas species ER72M2 and BAL-31. Virions adsorb via the distal tips of the spike proteins to uncharacterized receptors (Abrescia et al., 2008). The internal membrane mediates the translocation of the supercoiled genome across the host cell envelopes, most probably via fusion in a process that is not fully understood. Replication of the viral genome, most probably by a rolling circle mechanism, takes place in proximity to the host cytoplasmic membrane. The largest Pseudoalteromonas virus PM2 gene XII encoding protein P12 shares significant sequence similarity with the superfamily I group of replication initiation proteins (Männistö et al., 1999). The genome is organized in three operons (Figure 2.Corticoviridae). Operons OEL and OER encode early gene products: the replication initiation protein P12 and transcription regulatory proteins ...
To infect and replicate, PM2 delivers its genome across the cell envelope of two known marine host strains: Gram-negative Pseudoalteromonas species ER72M2 and BAL-31. Virions adsorb via the distal tips of the spike proteins to uncharacterized receptors (Abrescia et al., 2008). The internal membrane mediates the translocation of the supercoiled genome across the host cell envelopes most probably via fusion in a process which is not fully understood. Replication of the PM2 genome, most probably by a rolling circle mechanism, takes place in proximity to the host cytoplasmic membrane. The largest PM2 gene XII encoding protein P12 shares significant sequence similarity with the superfamily I group of replication initiation proteins (Männistö et al., 1999). The genome is organized in three operons (Figure 2.Corticoviridae). Operons OEL and OER encode early function gene products: the replication initiation protein P12 and transcription regulatory proteins P13, P14, P15 and P16. Expression of the ...
A total of 415 patients in whom Buruli ulcer has been clinically diagnosed will be included in the study, which will consist of 332 cases of category I and II Buruli ulcers (,10 cm) confirmed by polymerase chain reaction (PCR), plus 83 non PCR-confirmed Buruli ulcers. Patients will be randomized to receive treatment with the two antibiotic regimens as follows:. (i) Regimen I (SR8): 15 mg/kg streptomycin per day intramuscular injection for 8 weeks plus 10 mg/kg per day oral rifampicin for 8 weeks; (ii) Regimen II (CR8): 15 mg/kg per day oral extended-release clarithromycin for 8 weeks plus 10 mg/kg per day oral rifampicin for 8 weeks.. Assessments before, during and after the course of antibiotic treatment will include full medical history, clinical assessments and monitoring of vital signs, assessment of the lesion, laboratory investigations, hearing test, electrocardiogram, pregnancy test, voluntary HIV counseling and testing, and functional limitation assessment. The primary efficacy ...
Viruses are highly host specific.[5] Studies have shown that marine viruses are more likely to infect cooccurring organisms, those that live in the same region a virus exists in.[6] Therefore, biogeography is an important factor in a virions ability to infect. The knowledge of variation of viral populations across spatiotemporal and other environmental gradients is supported viral morphology, determined by transmission electron microscopy (TEM). Non-tailed viruses appear to be dominant in multiple depths and oceanic regions, followed by the Caudovirales myoviruses, podoviruses, and siphoviruses.[7] However, viruses belonging to families Corticoviridae,[8] Inoviridae[9] and Microviridae[10] are also known to infect diverse marine bacteria. Metagenomic evidence suggests that microviruses (icosahedral ssDNA phages) are particularly prevalent in marine habitats.[10] Metagenomic approaches to assess viral diversity are often limited by a lack of reference sequences, leaving many sequences ...
The in vivo oxidation of lipids and lipid-containing molecules has been discovered to be initiated by the concurrent reaction of such lipid materials with reducing sugars such as glucose, advanced gly
TY - JOUR. T1 - Bleomycin-induced Alkaline-labile Damage and Direct Strand Breakage of PM2 DNA. AU - Lloyd, R. Stephen. AU - Haidle, Charles W.. AU - Hewitt, Roger R.. PY - 1978/10. Y1 - 1978/10. N2 - Bleomycin-induced breakage of an isolated covalently closed circular DNA from bacteriophage PM2 was assayed fluorometrically after agarose gel electrophoresis and staining with ethidium bromide. When bleomycin-damaged DNA was assayed under neutral conditions, there was a decrease in the amount of Form I DNA and a simultaneous increase in both Forms II and III of DNA. However, when the damage was assayed under nondenaturing alkaline conditions, there was a greater decrease in the amount of Form I DNA and a corresponding increase in both Forms II and III DNA compared with neutral conditions. Approximately one alkaline-labile site was formed for every single-strand break introduced. The rate of formation of Form III DNA was found to be approximately twice as fast when measured under alkaline ...
SARS Vaccine Compositions and Methods of Making and Using Them - Described is a composition and method for reducing the occurrence and severity of infectious diseases, especially infectious diseases such as SARS, in which lipid-containing infectious viral organisms are found in biological fluids, such as blood. The present invention employs solvents useful for extracting lipids from the lipid-containing infectious viral organism thereby creating immunogenic modified, partially delipidated viral particles with reduced infectivity. The present invention provides delipidated viral vaccine compositions, such as therapeutic vaccine compositions, comprising these modified, partially delipidated viral particles with reduced infectivity, optionally combined with a pharmaceutically acceptable carrier or an immunostimulant. The vaccine composition is administered to a patient to provide protection against the lipid-containing infectious viral organism or, in case of a therapeutic vaccine, to treat or ...
Titering of Bacterial Viruses Related protocols: Preparation of Phage Stocks Commonly Used Media for Phage Growth Agar Overlay Technique When an individual bacterial virus grows in a bacterial host suspended in a top agar lawn, its progeny infect and lyse the surrounding host cells. This causes the appearance of a hole or plaque in…
Culture supernatant of Staphylococcus aureus, isolated from a patient who died after surgery induced granulation in several cultured cell lines of human and animal origin.
TAKEUCHI Osamu , KAWAI Taro , MUHLRADT Peter F. , MORR Michael , RADOLF Justin D. , ZYCHLINSKY Arturo , TAKEDA Kiyoshi , AKIRA Shizuo International immunology 13(7), 933-940, 2001-07-01 DOI 参考文献30件 被引用文献37件 ...
This invention is directed to systems and methods for removing lipids from a fluid, such as plasma, or from lipid-containing organisms. These systems contact a fluid with an extraction solvent, which causes the lipids in the fluid to separate from the fluid or causes lipids in the lipid-containing organisms to separate from the lipid-containing organism, using at least one hollow fiber contactor. The separated lipids are removed from the fluid. The extraction solvent is removed from the fluid or at least reduced to a level below a particular threshold enabling the fluid to be administered to a patient without the patient experiencing undesirable consequences. Once the fluid has been processed, the fluid may be administered to a patient who donated the fluid, to a different patient, or be stored.
Mycobacteria are distinguished from other micro-organisms by thick lipid-containing cell-walls that retain biochemical stains despite decolourisation by acid-containing reagents (so-called acid-fastness). Advantages: Microscopy of sputum smears is simple and inexpensive, quickly detecting infectious cases of pulmonary […]. ...
WASHINGTON, D.C. - The U.S. Agency for International Development (USAID) will host a forum on how sport is being used as an innovative and effective tool in international development and public engagement. The event will bring together high-profile speakers including Two Time All-Pro Wide Receiver with the Green Bay Packers Greg Jennings; former National Football League (NFL) Linebacker and founder of BowTieCause.org Dhani Jones; Phil de Picciotto, Founder & President Octagon, Inc. and USAID Administrator Rajiv Shah. The panel will be moderated by noted American political sportswriter Dave Zirin and will be preceded by remarks from former NFL Commissioner Paul Tagliabue.. ...
Cystovirus is a genus of viruses, in the family Cystoviridae. Pseudomonas syringae pathovar phaseolicola bacteria serve as natural hosts. There is currently only one species in this genus: the type species Pseudomonas phage phi6. Group: dsRNA Order: Unassigned Family: Cystoviridae Genus: Cystovirus Pseudomonas phage phi6 Pseudomonas phage phi8 Pseudomonas phage phi12 Pseudomonas phage phi13 Pseudomonas phage phi2954 Pseudomonas phage phiNN Pseudomonas phage phiYY Cystoviruses are distinguished by their tripartite dsRNA genome, totaling ~14 kb in length and their protein and lipid outer layer. No other bacteriophage has any lipid in its outer coat, though the Tectiviridae and the Corticoviridae have lipids within their capsids. Most identified cystoviruses infect Pseudomonas species, but this is likely biased due to the method of screening and enrichment. The type species is Pseudomonas phage phi6, but there are many other proposed members of this family. Pseudomonas phage φ7, φ8, φ9, φ10, ...
I have analyzed the time course of phage PR4 protein synthesis and have identified at least 34 proteins present in phage infected cells not detected in uninfected control cultures. In addition, I have isolated a more extensive set of conditional-lethal nonsense mutants of this virus. This collection of mutants permitted the identification of seven additional phage PR4 gene products, including the terminal genome protein and an accessory lytic factor. The present collection of phage PR4 mutants has been assigned to 19 distinct genetic groups on the basis of genetic complementation tests and sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the proteins produced in mutant infected UV irradiated cells ...
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Only recently have reproducible protocols for analyzing fecal phages emerged. A new review from University College Corks scientists explores the composition and relevance for health of the wide community of bacterial viruses colonizing our gastrointestinal tract.
Find great deals for The Molecular Biology of Bacterial Virus Systems by Springer-Verlag Berlin and Heidelberg GmbH & Co. KG (Paperback, 2011). Shop with confidence on eBay!
This was a fantastic opportunity to investigate the positive applications of bacterial viruses to fight infections. The recent and ongoing antibiotic-resistance crisis has heightened our awareness that we need to find alternative solutions to traditional antibiotic therapies. We isolated and tested the effectiveness of a number of bacterial viruses that can kill E. coli and Shigella, which are associated with gut infections. In the developed world, antibiotics have been used for decades to treat such infections rapidly but in developing countries, the water quality and sanitary conditions may create the perfect conditions for the growth of such illness-causing bacteria and antibiotic therapies may not be readily available. The lack of suitable treatments makes these infections potentially lethal, particularly to infants. Therefore, alternative treatments are required and bacterial viruses, or their encoded proteins, have the potential to be the next generation of antimicrobials to benefit all in ...
A team of Chinese and Danish researchers has identified 500 new species of gut-residing microorganisms and 800 new bacterial viruses which could attack them. The findings could lead to promising new t...
Calories in Betty Crocker Recipe Banana Bread. Find nutrition facts for Betty Crocker Recipe Banana Bread and over 2,000,000 other foods in MyFitnessPal.coms food database.