Human coronavirus NL63 or HCoV-NL63 is a species of coronavirus that was identified in late 2004 in a seven-month-old child with bronchiolitis in the Netherlands. Infection with the virus has been confirmed worldwide, and has an association with many common symptoms and diseases. Associated diseases include mild to moderate upper respiratory tract infections, severe lower respiratory tract infection, croup and bronchiolitis. The virus is found primarily in young children, the elderly, and immunocompromised patients with acute respiratory illness. It also has a seasonal association in temperate climates. A study performed in Amsterdam estimated the presence of HCoV-NL63 in approximately 4.7% of common respiratory illnesses. Further studies confirmed that the virus is not an emerging virus, but rather one that continually circulates the human population. The first cases of the infection with HCoV-NL63 were found in young children with severe lower respiratory tract infections admitted to ...

Acute respiratory tract infections in humans are responsible for significant morbidity and mortality especially in children, elderly, and the immunocompromised. Virus infection is the primary cause of acute respiratory tract infections. Infection with coronaviruses can cause disease ranging from common colds to severe acute respiratory syndrome. Currently no coronavirus-specific antivirals are available. Patients are given symptomatic treatment, or are prescribed inappropriately antibiotics which do not target the underlying virus infection. The identification of specific inhibitors of coronaviruses or knowledge about how coronaviruses interact with the innate immune system could provide new avenues for developing therapeutics. I investigated the replication of human coronavirus NL63 (HCoV-NL63) in primary human airway epithelial cultures and characterized the activity of the viral papain-like protease 2 (PLP2) domain. Using quantitative real time PCR and imaging techniques I showed that HCoV-NL63

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To date, several coronaviruses (including SARS-CoV, MERS-CoV, and SARS-CoV-2) that could infect humans have all been beta-coronaviruses (1,8). The risk associated with false-positive PCR results posed a challenge in clinical application. First, the primer dimers and non-specific amplification, in which probe sequences participate, might interfere with experiments. Second, SARS-CoV-2 assays might cross-react with other viruses. In this study, we used 6 human coronaviruses (NL63-CoV, 229E-CoV, OC43-CoV, HKU1-CoV, SARS-CoV and MERS-CoV) and influenza A to test the cross-reactivity. Consequentially, the ORF1ab and N gene-based assay were the most specific, exactly matching target genes of SARS-CoV-2. The E gene was cross-reacted with B lineages of the beta-coronavirus (such as SARS-CoV). We propose that the E gene could be used as a broad-spectrum screening gene for B lineages of the beta-coronavirus, such as SARS-CoV, SARS-CoV-2, bat SARS-like coronavirus, et al. Therefore, we currently prefer to ...

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Shimizu, C., Shike, H., Baker, S. C., Garcia, F., van der Hoek, L., Kuijpers, T. W., Reed, S. L., Rowley, A. H., Shulman, S. T., Talbot, H. K. B., Williams, J. V., and Burns, J. C., 2005, Human coronavirus NL63 is not detected in the respiratory tract of children with acute Kawasaki disease, J. Infect. Dis. 192: 1767-1771CrossRefPubMedGoogle Scholar ...

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GBIF data worden gebruikt in wetenschappelijke publicaties. Deze pagina verschaft een overzicht, via de literatuur verzamel site Mendeley, van publicaties die GBIF noemen of die gebaseerd zijn op GBIF data.. ...

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Recombinant Human coronavirus SARS Nucleoprotein is an Escherichia coli Protein fragment 1 to 49 aa range, | 95% purity and validated in WB, ELISA, SDS-PAGE.

Poppe, M.; Wittig, S.; Jurida, L.; Bartkuhn, M.; Wilhelm, J.; Müller, H.; Beuerlein, K.; Karl, N.; Bhuju, S.; Ziebuhr, J.; Schmitz, M.Lienhard.; Kracht, M., 2017: The NF-κB-dependent and -independent transcriptome and chromatin landscapes of human coronavirus 229E-infected cells

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Human coronavirus. Coloured transmission electron micrograph (TEM) of Human coronavirus particles (purple circles). Coronaviruses primarily infect the upper respiratory and gastrointestinal tract and can cause the common cold, gastrointestinal infections and SARS (severe acute respiratory syndrome). Coronaviruses are named after the corona (crown) of surface proteins (dark dots) that are used to penetrate a host cell. Once inside the cell, the virus particles (virions) use the cells machinery to make more copies of themselves. Magnification: x67,000 when printed 10 centimetres tall. - Stock Image C001/0467

The four endemic human coronaviruses HCoV-229E, -NL63, -OC43, and -HKU1 contribute a considerable share of upper and lower respiratory tract infections in adults and children. While their clinical representation resembles that of many other agents of the common cold, their evolutionary histories, an …

Human coronaviruses can sometimes cause lower-respiratory tract illnesses, such as pneumonia or bronchitis. This is more common in people with cardiopulmonary disease, people with weakened immune systems, infants, and older adults.. Other human coronaviruses. Two other human coronaviruses, MERS-CoV and SARS-CoV have been known to frequently cause severe symptoms. MERS symptoms usually include fever, cough, and shortness of breath which often progress to pneumonia. About 3 or 4 out of every 10 patients reported with MERS have died. MERS cases continue to occur, primarily in the Arabian Peninsula. SARS symptoms often included fever, chills, and body aches which usually progressed to pneumonia. No human cases of SARS have been reported anywhere in the world since 2004.. Transmission. Human coronaviruses most commonly spread from an infected person to others through the air by coughing and sneezing close personal contact, such as touching or shaking hands touching an object or surface with the virus ...

Human coronaviruses (HCV) cause various respiratory, gastrointestinal and possibly neurological disorders. Very little is known of the molecular biology of these ubiquitous pathogens. We have...

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Coronaviruses were classified as a distinct group of viruses in 19681 and are now recognized as the etiologic agents of an increasing number of diseases of man and animals2-7. At least four members...

Pandemics, even more than other medical problems, require swift integration of knowledge. When caused by a new virus, understanding the underlying biology may help finding solutions. In a setting where there are a large number of loosely related projects and initiatives, we need common ground, also known as a

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John Yin is working to find out whether junk particles produced by mouse viruses exist in human coronaviruses, and whether they may be the key to understanding how the viruses spread and interact with host cells.. ...

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