Summary Complement fixation tests with a purified extract of adult Schistosoma mansoni were performed by a quantitative technique with the sera of 216 children (10 to 15 years of age) and 84 adults (20 to 40 years of age) passing eggs of S. mansoni in their stools. The test was positive in 88.9% of the children and 89.3% of the adults. The titers obtained for the two age groups did not differ significantly.
TY - JOUR. T1 - Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders. AU - Elschner, Mandy Carolina. AU - Laroucau, Karine. AU - Singha, Harisankar. AU - Tripathi, Bhupendra Nath. AU - Saqib, Muhammad. AU - Gardner, Ian. AU - Saini, Sheetal. AU - Kumar, Subodh. AU - El-Adawy, Hosny. AU - Melzer, Falk. AU - Khan, Iahtasham. AU - Malik, Praveen. AU - Sauter- Louis, Carola. AU - Neubauer, Heinrich. PY - 2019/4/1. Y1 - 2019/4/1. N2 - Glanders is a zoonotic contagious disease of equids caused by Burkholderia (B.) mallei. Serodiagnosis of the disease is challenging because of false-positive and false-negative test results. The accuracy of the complement fixation test (CFT) which is prescribed for international trade by the World Organisation for Animal Health (OIE), five ELISAs and a Western blot (WB) were compared for serodiagnosis of glanders using sera from 3,000 glandersfree and 254 glanderous ...
Looking for complement-fixing antibody? Find out information about complement-fixing antibody. protein produced by the immune system in response to the presence in the body of antigens: foreign proteins or polysaccharides such as bacteria, bacterial... Explanation of complement-fixing antibody
Wilson joined the UC Berkeley faculty of biochemistry in 1964, and was promoted to full professor in 1972.[8] His first major scientific contribution was published as Immunological Time-Scale For Hominid Evolution in the journal Science in December 1967.[16] With his student Vincent Sarich,[17][18] he showed that evolutionary relationships of the human species with other primates, in particular the Great apes (chimpanzees, gorillas, and orangutans), could be inferred from molecular evidence obtained from living species, rather than solely from fossils of extinct creatures. Their microcomplement fixation method (see complement system) measured the strength of the immune reaction between an antigen (serum albumin) from one species and an antibody raised against the same antigen in another species. The strength of the antibody-antigen reaction was known to be stronger between more closely related species: their innovation was to measure it quantitatively among many species pairs as an ...
Rapporteur: Grant McIntyre. Copes Rule states that body size increases during the evolutionary history of a lineage. This may be true from certain perspectives but is no longer generally accepted. The lecture examined several studies on the evolution of body size that shed light on the prevalence of exceptions to Copes Rule and concluded with a discussion of reasons for the apparent support for the rule.. The focal paper (Pianka 1995) used Felsensteins method of independent contrasts to evaluate the evolution of body size of varanid (monitor) lizards. This method requires a phylogeny which was developed in this case using immunological distances determine by microcomplement fixation. The phylogeny matched species distributions fairly well but was not rooted since no available outgroup cross-reacted immunologically with the ingroup. Within varanids there are examples of the evolution of both larger and smaller size. The main problem with the paper was that lack of an outgroup precluded ...
CANDEIAS, J. A. N.; STEWIEN, Klaus E. and BARBOSA, Victório. Serological study of cytomegalovirus infections. Rev. Saúde Pública [online]. 1974, vol.8, n.3, pp.257-263. ISSN 1518-8787. http://dx.doi.org/10.1590/S0034-89101974000300002.. The age and sex distribution of complement-fixing antibodies was studied in a sample of 1294 sera from adults and children of S. Paulo city, Brazil. The incidence of 33% by 15 years of age, with a maximum of 70% reached in the older age group (, 35 years), support the findings in other countries. An analysis of sera in terms of sex showed no significant differences in the distribution of complement-fixing antibodies to CMV. A comparative age distribution of CMV complement-fixing and neutralizing antibodies in a sample of 236 sera, using the AD 169 strain, confirmed the high correlation between these types of antibodies, being the per cent of positive sera consistently higher for neutralizing antibodies.. Keywords : Cytomegalovirus infections; Serological ...
There is no reliable clinical method to distinguish coronavirus colds from colds caused by rhinoviruses or less common agents. For research purposes, virus can be cultured from nasal swabs or washings by inoculating organ cultures of human fetal or nasal tracheal epithelium. The virus in these cultures is detected by electron microscopy or other methods. The most useful method for laboratory diagnosis is to collect paired sera (from the acute and convalescent phases of the disease) and to test by ELISA for a rise in antibodies against OC43 and 229E. Complement fixation tests are insensitive; other tests are inconvenient and can be used only for one serotype. Direct hybridization and polymerase chain reaction tests for viral nucleic acid have been developed and, particularly with the latter, are the most sensitive assays currently available for detecting virus .. ...
Comparision has been made between two human "neuropathogenic" and one "commensal" strains of herpes simplex virus. These terms are assigned to these viruses on the basis of their origin. The first two were related from patients with encephalitis and the third from a benign recurrent lesion. A number of physiochemical characteristics of these three strains were studied. The major differences observed between the "pathogens" and the "commensal" virus are: 1. The ability of the commensal herpes virus to infect adult mice while the pathogenic herpes could not. 2. The commensal herpes virus could not be neutralized by the hyperimmune sera of the pathogenic herpes and vice versa, although all crossreacted in complement fixation tests. 3. The commensal virus was significantly inactivated by chloroform, while the pathogens were not significantly affected under the same conditions. 4. Freezing and thawing followed by differential centrifugation rendered the commensal herpes vulnerable to inactivation of ...
Synonyms for Bordet, Jules in Free Thesaurus. Antonyms for Bordet, Jules. 3 words related to complement fixation: immune reaction, immune response, immunologic response. What are synonyms for Bordet, Jules?
Summary and Conclusions A complement-fixation test, using an antigen prepared from infectious mouse brains, is described for yellow fever. The test has been shown to be biologically specific. Nonspecific reactions, however, tend to occur with Wassermann-positive sera, but these can usually be avoided by inactivating the sera at 65°C. Complement-fixing antibodies were demonstrable in the serum of humans and monkeys recovered from infection with yellow fever virus. Following vaccination with the 17D strain, complement-fixing antibodies were uniformly present in monkey serum, but were detectable in only a small proportion of human sera. The application of this latter fact in making yellow fever surveys is discussed.
The complement fixation test is based on the use of complement, a biological substance present in the sera of normal animals. Its great value is its predictable activity in the presence of serologically reacting factors and its nonspecificity, that is, it is not like an antibody with a narrow range of reactions or increased concentration occurring in a host following immunization or infection. Furthermore, it is easily destroyed by heating at temperatures that have no deleterious effect on antibodies.
3 pages pp. 410-413 IN: Proceedings of the Society for Experimental Biology and Medicine, Volume. 44, no. 2. Thick 8vo, modern wrappers. N.Y., 1940. First Edition. Whole number 2 offered entire. Diagnosis of lymphogranuloma venereum by complement-fixation test. With G.W. Rake and M.F. Shaffer. GM 5223. Binding: Paperback Language: English
In 1979 an indirect haemagglutination test (gonococcal antibody test) using gonococcal pilus antigen replaced the gonococcal complement fixation test as our routine procedure to show gonococcal antibodies. In the diagnosis of current gonorrhoea the sensitivity of the gonococcal antibody test was far superior to that of the gonococcal complement fixation test (about 55% versus 9% for first episode gonorrhoea). To evaluate the usefulness of the test result the following population groups were studied: 1376 patients undergoing medical examination for gonorrhoea (386 had gonorrhoea), 1384 healthy people aged 15-65, 54 patients with meningococcal disease, 30 children with respiratory tract infection, and 254 patients with evidence of various diseases other than neisserial infections that might be associated with symptoms of arthritis. These investigations showed that (1) non-specific positive gonococcal antibody test results occur rarely, (2) at least half the people who have had gonorrhoea remain ...
Banked acute-phase and convalescent-phase serum samples from a previous study of respiratory illness in university students were examined for significant (≥2-fold) increases in ELISA titers of IgA and IgG antibody to Bordetella pertussis filamentous hemagglutinin, pertactin, and fimbriae-2 and ≥4-fold titer increases to agglutinogens by agglutination. ELISA titers of antibody to pertussis toxin could not be determined because of technical problems. Chlamydia pneumoniae infections were diagnosed by culture or by a ≥4-fold increase in immunofluorescence assay titer or a single high titer (≥512). Mycoplasma pneumoniae, influenza A and B, adenovirus, and respiratory syncytial virus infections were diagnosed by ≥4-fold increases in complement fixation titer or a single high titer (≥64). There were 319 subjects with cough of ≥5 days duration, and of these, 47 (15%) had significant increases in antibody to B. pertussis antigens; 26 (8%) had significant increases to fimbriae-2 or ...
Michaelsen, Terje Einar; Gilje, A; Samuelsen, Anne Berit; Høgaasen, K & Paulsen, BS (2000). Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L. Scandinavian Journal of Immunology. ISSN 0300-9475. 52, s 482- 490 Vis sammendrag The interaction between a pectin type polysaccharide fraction, PMII, isolated from the leaves of Plantago major, and human complement was tested in two different hemolytic complement-fixation tests and in addition by two ELISA methods detecting complement-activation products. Sera were used as a complement source of 10 arbitrary human volunteers, individually and as a pool. The complement-fixation tests were designed to measure the concentration of the pectin necessary to inhibit 50% of the hemolysis (ICH(50)). The ELISA tests for complement-activation products were measured in AU/mg using a fully activated serum as a standard. We observed a more than 200-fold difference in ICH(50) activity of the PMII ...
Canine hepatitis is an infectious disease. This disease is found in dogs, wolves, coyotes, foxes, and in bears. This virus directly attacks on the kidney and liver of the animal. The word hepatitis is originated from the Greek word, which means the inflammation of the liver, caused by the infectious toxic. This disease is very Read more ...
When filtered alkaline extracts of pulverized bacteria of several varieties are precipitated with acid in the cold, boiled with acid, and all materials thrown down by these procedures removed, there remains a small amount of an alcohol-precipitable material which no longer gives any of the ordinary chemical reactions for proteins, such as the biuret, Hopkins-Cole, Millon, and sulfosalicylic acid reactions. The only protein reaction usually given by this material is a very weak xanthoproteic reaction. Nevertheless, the material, which is, as far as we can determine at present, free from coagulable protein, is specifically precipitable by homologous antiserum and gives specific complement fixation reactions.. Such material can also be obtained from organisms like the influenza bacillus, pneumococcus, and meningococcus by extraction without preliminary grinding of the bacteria, and is present in filtrates of young and old broth cultures of the organisms.. We believe that these acid- and ...
Modified indirect hemagglutination test for detection of treponemal antibodies in finger-prick blood.: A modified indirect hemagglutination test for the detecti
Thirty brucellosis free calves with zero titres to the serum agglutination test (SAT), complement fixation test (CFT) and antiglobulin test (ABGT) were vaccinated with strain 19 at ages from seven hours to 198 days. Calves 75 days of age and older responded with normal serological patterns, developing high titres to all three tests. At 45 days and younger most calves responded with much reduced titres, some were negative to the SAT and CFT but all develped titres to the ABGT. Two of the younger group were subjected to an anamnestic test at about a year old and gave a positive response, indicating that the calf may be effectively primed with S19 as early as the first day of life. Three of the group were colostrumdeprived yet the patterns of their responses were similar to those of the colostrum-fed calves. Seventy-four zero titres calves were vaccinated with killed 45/20 adjuvant vaccine at ages from 60 to 320 days. Up to 200 days of age only seven of 33 calves gave positive response. From 200 to ...
Background: In patients (pts) who are highly sensitized, desensitization therapy is commonly applied to reduce antibody (Abs) load and to reduce potential morbidity and mortality following heart transplant (HTx). It is not known as to what level of antibody binding is needed to initiate desensitization therapy. It is believed that high binding Abs may have the ability to fix complement and lead to cytotoxicity.. Methods: Between 2011 and 2012 we assessed 13 highly sensitized pts who were noted to have standardized fluorescence intensity (SFI) ,100,000 pretransplant.. A complement binding assay (C1q binding assay) was then applied to these Abs to assess for a correlation of binding to cytotoxicity. Correlation statistics were used to derive significance between higher antibody binding and capacity to bind complement.. Results: There seems to be no correlation between the different binding strength and C1q positivity. However, when evaluating Luminex 1:8 test, there seems to be correlation between ...
Date: 1906. : the process of binding serum complement to the product formed by the union of an antibody and the antigen for which it is specific that occurs when complement is.
This clearance establishes Tm as a unique supplier of CF tests to our rapidly expanding customer base against such competitors as ABI (NYSE:ABI - News) and Third Wave (NASDAQ:TWTI - News)," said Greg Hines, President and CEO of Tm Bioscience. "The Tag-It(TM) Cystic Fibrosis Kit is the only CF testing system that has performance characteristics which have been established through extensive studies reviewed by the FDA. Having the first CF test and second multiplexed genetic test behind Roches (RHHBF.PK) AmpliChip CYP450 to be cleared as an IVD, sets the regulatory pathway for other tests in our broad and growing pipeline and positions Tm as a leader in the commercial genetic testing market ...
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Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.
Effects of adding phenol to sera used for the card-agglutination test (CAT) and for the micro-complement-fixation test (CFT) for bovine anaplasmosis were studied. Sera were obtained from 14 recently infected cattle, 17 cattle vaccinated with a killed anaplasmosis vaccine, 5 cattle in the carrier phase of the disease, and 45 cattle of unknown anaplasmosis status. Aliquots of sera were tested with and without phenol (0.25% final concentration). Phenol adversely affected the CAT by causing false-negative results. The CAT reactions of nonphenolized sera from recently infected cattle were all positive 4 weeks after inoculation, whereas CAT reactions of phenolized sera were not all positive until 10 weeks after inoculation. Nine non-phenolized sera from vaccinated cattle that were CAT-positive were CAT-negative after being phenolized. Phenolized sera from carrier cattle and from cattle of unknown anaplasmosis status were less reactive on the CAT than were nonphenolized sera. Effects of phenol on the CFT were
A study of 531 female patients attending a venereal disease clinic was undertaken to assess the incidence of cytomegalovirus (CMV) in the cervix. The findings were as follows: (1) 35 of 531 patients had positive cervical cultures for CMV (6-6 per cent.). (2) 28 of 531 patients were positive for Herpes virus hominis (5-3 per cent.). (3) Excluding those who were pregnant, 20 of 28 with CMV were taking oral contraceptives (71 per cent.). (4) Seven babies born to infected mothers showed no signs of cytomegalic inclusion disease. (5) 28 of 35 with CMV had associated genital infections (80 per cent.). (6) Positive cultures were obtained in twenty cases for periods up to 10 months. (7) The CMV complement-fixation test was positive in all 23 patients with positive CMV cultures who were tested. (8) Seven male consorts were examined but CMV was not isolated from any of them. (9) A case of CMV mononucleosis was detected. It is suggested that the higher incidence in patients attending a VD clinic is due to ...
EPFL scientists have developed a uranium-based complex that allows nitrogen fixation reactions to take place in ambient conditions. The work lays the foundation to develop new processes for synthesizing nitrogen products like cyanamide.
The human endometrial epithelial cancer cell line Hec50 (Holinka et al., 1996) was used to prepare chromatin for X-ChIP and N-ChIP assays according to the Chromatrap® protocols. For the N-ChIP assay, 1 x 106 cells were grown to a confluency of 80%, scraped in ice-cold PBS, and finally collected through centrifugation. Cell lysis was performed in Hypotonic Buffer, before subjecting the separated nuclei to micrococcal nuclease digestion to yield chromatin fragments between 100 and 500 bp in length. Sample dialysis was carried out to remove unwanted impurities and these samples were processed for the ChIP method. For the X-ChIP assay, 1 x 106 cells were grown to a confluency of 80% and fixed in 1% formaldehyde for a period of 10 minutes. Glycine was used to quench the fixation reaction and cells were gathered in ice-cold PBS and then spun down, discarding the supernatant prior to re-suspension and lysis in Hypotonic Buffer. The nuclei thus released were gathered by centrifugation and lysed, and ...
Med. 85, 202. 5. Tang, F. , Chang, H. , Huang, Y. , Wang, K. C. (1957) Chinese Med. J. 75, 429. 6. Burnet, F. M. (1936) Spec. Rep. Series, Med. Res. Council, London, No. 220. 7. Cox, H. R. (1952) Ann. Y. Acad. Sei. 55, 236. 8. Hirst, G. K. (1945) Proc. Soc. Exp. Biol. Med. 58, 155. 9. Burnet, F. M. (1940) Brit. J. Exper. Path. 21, 147. 10. Beveridge, W. I. , Burnet, F. M. (1946) Spec. Rep. Series, Med. Res. Council, London, No. 256. 11. Levens, J. , Enders, J. F. (1945) Science, 102, 117. 12. Enders, J. J. Hyg. 60, 214. 28. Porterfield, J. S. (1960) Bull. Wld. Hlth. Org. 22, 373. 29. Cooper, P. D. (1961) Advances in Virus Research, 8, 319. CHAPTER SIX SEROLOGY THERE are five main types of serological reaction associated with viral investigations. Three of these, neutralisation, haemagglutinationinhibition (HI) and complement fixation (C-F) are used principally for diagnosis and epidemiology. The other two, agar-gel diffusion and fluorescent antibody staining are primarily research tools, only ...
Serpin B9 is a protein that in humans is encoded by the SERPINB9 gene.[1][2][3] PI9 belongs to the large superfamily of serine proteinase inhibitors (serpins), which bind to and inactivate serine proteinases. These interactions are involved in many cellular processes, including coagulation, fibrinolysis, complement fixation, matrix remodeling, and apoptosis (Sprecher et al., 1995).[supplied by OMIM][3] ...
1. A specific antibody, demonstrable by the technique of complement fixation, regularly appears, or increases in concentration, in the sera of human beings during an attack of mumps or during convalescence.. 2. Specific dermal hypersensitivity, demonstrable by the injection of heat-inactivated mumps virus, has been shown to develop in 6 human beings after recovery from mumps.. 3. Complement-fixing antibody and the hypersensitive state also emerge as a result of clinically inapparent infection with the virus of mumps.. 4. These two phenomena are apparently unrelated in respect to immunologic mechanisms.. 5. The data presented indicate that the complement fixation test should prove of value both in diagnosis and in the determination of immunity.. 6. The skin test for dermal hypersensitivity, on the other hand, becomes positive after recovery and therefore would appear to be useful only as an index of resistance.. ...
Abstract: : Purpose:We reported that patients with Vogt-Koyanagi-Harada (VKH) disease had immune responses specific to human melanocyte antigens, tyrosinase and gp100. This study was aimed at analyzing whether lymphocytes of patients with VKH disease have a cross-reaction between melanocyte peptides and virus antigens. Methods:The subjects of this study were patients with VKH disease and patients with other uveitis in 1989-2001at our hospital. We searched molecular mimicry between tyrosinase450-462, gp10044-59 and exogenous antigen, such as virus, using database screening. Then, crossreactivity to melanocyte peptides and virus antigen were examined by lymphocyte proliferation assay. The seroprevalence of various viruses in patients with VKH disease (n=109) was examined by complement fixation test. In order to examine if the virus infection in VKH patients were latent, genomic DNA of virus was measured by quantitative PCR using samples from serum, cerebro-spinal fluid, and aqueous humor. All ...
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The immunomodulating effects of isolated proanthocyanidin-rich fractions, procyanidins C1, B5 and B2 and anthocyanins of Aronia melanocarpa were investigated. In this work, the complement-modulating activities, the inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages and effects on cell viability of these polyphenols were studied. Several of the proanthocyanidin-rich fractions, the procyanidins C1, B5 and B2 and the cyanidin aglycone possessed strong complement-fixing activities. Cyanidin 3-glucoside possessed stronger activity than the other anthocyanins. Procyanidins C1, B5 and B2 and proanthocyanidin-rich fractions having an average degree of polymerization (PD) of 7 and 34 showed inhibitory activities on NO production in LPS-stimulated RAW 264.7 mouse macrophages. All, except for the fraction containing proanthocyanidins with PD 34, showed inhibitory effects without affecting cell viability. This study suggests that polyphenolic compounds of A. melanocarpa may
Chlamydia pecorum and Chlamydia abortus are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low C. abortus seropositivity with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of C. abortus -associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only C. pecorum DNA could be detected by qPCR in Chlamydia seropositive Australian animals screened, suggesting chlamydial seropositivity was due to cross-reactivity with endemic C. pecorum infections. These results suggest ascribing Chlamydia seropositivity to chlamydial species in livestock using whole-cell ...
An Oropouche virus strain was isolated from a novel host (Callithrix sp.) in Arinos, Minas Gerais State, southeastern Brazil. The virus was identified by complement fixation test and confirmed by reverse transcription-polymerase chain reaction. Phylogenetic analysis identified this strain as a genotype III isolate previously recognized only in Panama.
A latex fixation test, also called a latex agglutination assay or test (LA assay or test), is an assay used clinically in the identification and typing of many important microorganisms. These tests use the patients antigen-antibody immune response. This response occurs when the body detects a pathogen and forms an antibody specific to an identified antigen (a protein configuration) present on the surface of the pathogen. Agglutination tests, specific to a variety of pathogens, can be designed and manufactured for clinicians by coating microbeads of latex with pathogen-specific antigens or antibodies. In performing a test, laboratory clinicians will mix a patients cerebrospinal fluid, serum or urine with the coated latex particles in serial dilutions with normal saline (important to avoid the prozone effect) and observe for agglutination (clumping). Agglutination of the beads in any of the dilutions is considered a positive result, confirming either that the patients body has produced the ...
This is the first comprehensive report of serologic responses to the oral ETEC-rCTB vaccine in subjects living in an area where ETEC is endemic. We consider the pediatric data presented herein especially relevant, since children and infants in Egypt and other developing countries represent important targets for ETEC immunization. Several of our findings bear importance for future evaluations of the ETEC-rCTB vaccine. First, the vaccine elicited pronounced antitoxin antibody responses of both IgA and IgG isotypes in all three age groups as reported here and elsewhere (15). Second, a high proportion of vaccinated children achieved seroconversion to each of the four vaccine-shared CF antigens studied, and the magnitude of antibody elevations, particularly of IgA isotype, was substantial. Third, both age and preimmunization titer were independently and inversely associated with the magnitude of IgA antibody responses to the vaccine. Last, IgG antibodies to CF antigens appeared to reflect a ...
Antisera against hexons of serotypes 2, 4, 5, and 6 (subgroup III), and 15 (subgroup II) were absorbed with purified hexons of various serotypes representing the different subgroups of human adenoviruses. Group, subgroup, and type specificities of hexons could be distinguished. The subgroup specificity of type 4 hexons resembled that of hexons of subgroup I members (types 3, 11, and 16). Antihexon sera gave a type-specific inhibition of virion-associated hemagglutinin. The inhibiting activity of different sera was found to be inversely related to the length of fibers of the serotype concerned. Virions of serotypes carrying fibers shorter than about 20 nm (types 3, 4, 9, 11, and 15) were readily inhibited, whereas those of serotypes with longer fibers (types 2 and 6) were inhibited only by relatively large amounts of antibody measured in terms of homotypic complement fixation activity. The reciprocal cross-neutralization between serotypes 4 and 16 was studied separately. Hexons of both serotypes ...
Mechanisms for the elimination of potentially lytic complement-fixing variable surface glycoprotein antibody complexes in Trypanosoma ...
Dendritic polyglycerol sulfate (dPGS) has originally been investigated as an anticoagulant to potentially substitute for the natural glycosaminoglycan heparin. Compared to unfractionated heparin, dPGS possesses lower anticoagulant activity but a much higher anticomplementary effect. Since coagulation, complement activation, and inflammation are often present in the pathophysiology of numerous diseases, dPGS polymers with both anticoagulant and anticomplementary activities represent promising candidates for the development of polymeric drugs of nanosized architecture. In this review, we describe the nanomedical applications of dPGS based on its anti-inflammatory activity. Furthermore, the application of dPGS as a carrier molecule for diagnostic molecules and therapeutic drugs is reviewed, based on the ability to target tumors and localize in tumor cells. Finally, the application of dPGS for inhibition of virus infections is described.
Adiga, PR (1981) Biochemical And Immunological Studies On Riboflavin-Carrier Protein (Rcp) Essential For Fetal Survival In The Pregnant Rat - A New Approach To Fertility-Control. In: Indian Journal of Biochemistry & Biophysics, 18 (4). pp. 36-37. Full text not available from this repository ...
The Virological Department of the Hammersmith Hospital for the past two years has screened for the presence of Australia antigen (HAA) all candidates for admiss
Dilithium oxide | HLi2O+ | CID 82891 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
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Parallel testing of 895 sera by indirect hemagglutination and indirect fluorescent-antibody techniques showed 97.3% agreement. Although the indirect hemagglutination technique usually showed more cross-reactivity among serogroups than the indirect fluorescent-antibody technique with Formalin-fixed antigens and a conjugate which detected primarily immunoglobulin G antibodies, heterologous serogroup reactions were significantly lower than homologous serogroup titers and the etiological serogroup could be easily defined. The indirect hemagglutination techniques showed no cross-reactivity with a crude extract of Escherichia coli O13:K92:H4. Since the indirect hemagglutination technique was shown to detect both immunoglobulin M and immunoglobulin G antibodies and was found to be rapid, simple, and inexpensive, it appears to be an excellent alternative to the indirect fluorescent-antibody test for serodiagnosis of legionellosis. ...
Between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. This total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. The serum samples were tested using the Rose Bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. Moreover, a complete history was compiled from each flock/herd. The rate of abortions in sheep due to brucellosis ranged from 0.5% to 56%, with a mean of 33.2%. The goats had a higher abortion rate. Thirty-four aborted sheep foetuses collected from these 20 flocks were bacteriologically and pathologically examined. A pure culture of Brucella melitensis biotype 3 was isolated from 21 of the aborted foetuses.. ...
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
An unrecognized epidemic of Mycoplasma pneumoniae infection in Vienna.: Mycoplasma pneumoniae infection shows epidemiological peaks with a 2- to 10-fold increas
Mycoplasma pneumoniae - common cause of mild pneumonia, bronchitis and sore throat - usually resolves without any serious complications