Abstract on continuous flow perfusion of gentamicin with a (Morgan Lens) reduces bacterial colony counts in experimental pseudomonas keratitis.
INTRODUCTION The degradation of organic matter in soil largely results from the interaction between macro- and microorganisms, although microorganisms have a greater participation in this process because of their higher biomass values (7). However, to determine the microbiological characteristics of soil, samples should be promptly analyzed after collection to avoid the risk of obtaining altered results due to the variation in number of microorganisms when the samples are maintained at room temperature. If microbial analysis cannot be performed immediately after collection, Clark (3) recommends conservation at 4oC for a maximum period of 1-2 weeks. Higashida and Takao (8) performed microbial counts in soil samples up to approximately 8 hours after collection. In support of this view, Harding and Ross (6) reported that fresh soil samples are desirable to characterize microbial counts. If the procedures can not be carried out immediately, some form of storage should be used. Dunn et al. (4) and ...
Many studies require the quantitative determination of bacterial populations. The two most widely used methods for determining bacterial numbers are: The standard plate count method. Spectrophotometer (turbid metric) analysis. The standard plate count method is an indirect measurement of cell density ( live bacteria). The spectrophotometer analysis is based on turbidity and indirectly measures all bacteria (cell biomass), dead and alive.
새우를 키토산 단독 코팅하거나 천연항균제(carvacrol, thymol)를 키토산 코팅과 병행 처리하였을 때 중온균수의 변화를 Table 1에 나타내었다. 중온균은 모든 처리구에서 초기 농도가 1.33 log CFU/g이었고 냉장 저장 1일째 1.0 log CFU/g까지 감소되었으나, 6일째부터 계속 증가하기 시작 하였다. 저장기간 중 가장 마지막 날인 12일째에, Control, Chi, Chi-Car 및 Chi-Thy의 중온균수는 각각 5.25, 3.59, 3.29, 1.97 log CFU/g로 모든 처리구가 대조구에 비해 유의 적으로(p , 0.05) 낮은 중온균 수를 나타내어 항균효과가 있음을 알 수 있었다. 그리나 12일째에도 모든 실험구의 총균수는 수용가능한 수준18)인 7 log CFU/g을 초과하지는 않았다. 6일째부터 처리구 간에 유의적 차이가 발생하였 으며, Chi-Thy, Chi-Car, Chi의 순서대로 강한 항균효과를 보였다.. 새우를 키토산 단독 코팅하거나 ...
Dr B.R. Gupta has conducted experiments on effects of Agnihotra on plate count of Aerial bacterial flora. He has found that in human residence where no Agnihotra was performed the bacterial colony count was 123 as against in the human residence where Agnihotra was done regularly the bacterial colony count was as low as a mere 25.. ...
Determined to prove the performance of its own products in a medical environment, Rehau recently commissioned a microbiological analysis of BioCote treated Rehau cable trunking installed at the newly opened Churchfields GP Surgery in Bromsgrove, Worcestershire, and compared it with cable trunking installed at the previous Churchfields surgery, which had been out of use for several months.. The results were hugely impressive with a bacterial colony count (CFU) of just three on the cable trunking in the new surgery compared with 228 in the old one - a reduction of 98.69 per cent.. To give an even clearer indication of the performance of the trunking, Rehau also measured the bacterial colony count on the comparable door handle in the new surgery which showed a level of 215 and on the windowsill which showed a level of 160.. Overall, the comparative study between the two buildings showed that the new surgery, which had been open for two months, was in general less contaminated than the old one with ...
The fundamental principle of the microbial challenge (PET) is based on the concept of measuring the survival ability of selected microorganisms that are purposely introduced into a preserved test product system. The test product containing the inhibitory system is inoculated with the desired pool at the target level of bacteria (105-6 cfu/gram) and yeasts and molds (105-4cfu/gram). At the desired sampling interval (day 0, 7, 14 and 28 days) a sample is analyzed to determine the reduction in counts. The vial is tested on the BioLumix instrument for 22 hours for bacteria and for 48 hours for yeast and molds. The DTs are converted to Log cfu/g. and the Log reduction is calculated. The main advantage of this method is the automation of the process and the labor savings that it offers. It was estimated that it takes ~ 1/5 of the labor to perform the BioLumix assay compared to the standard plate count method. The new BioLumix assay offers savings in time, labor, materials, and plate space. Please ...
Looking for colony count? Find out information about colony count. The number of colonies of bacteria growing on the surface of a solid medium Explanation of colony count
Current routine analysis of the microbiological quality of drinking water is very often based on plate count methods, where the number of bacterial colonies on a nutrient medium are counted. This method has been applied worldwide for more than 100 years. Despite its ubiquitous use, this method is rather time consuming. It takes approximately 3 days to detect microbiological contamination of drinking water. The detection of additional pathogens, such as Legionella, can easily take several days to weeks. A further disadvantage of culture methods lies in the fact, that only a minor fraction of microorganisms found in environmental samples (0.1-1%) grow in culture media and can therefore be detected. A much rapid and more complete method for the analysis of the microbiological quality of water resources and drinking water is provided by Flow Cytometry. The potential and the limits of this technique have been investigated in detail in the last few years by the research group of Prof. Thomas Egli in ...
The microbial load of cassava tubers that were grown at Awassa, Ethiopia, was analysed. The total viable microbial count of fresh cassava, before cleaning, ranged from 8.7x104 to 2.1x109 ...
It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review …
Compare the average total cost at facilities in Maine for the procedure: Urine test for bacterial culture: quantitative colony count.
This International Standard gives guidelines for the evaluation of uncertainty in quantitative microbiological analyses based on enumeration of microbial particles by culture. It covers all variants of colony count methods and most probable number estimates. Two approaches, the component (also known as bottom-up or step-by-step) and a modified global (top-down) approach are included. The aim is to specify how values of intralaboratory operational variability and combined uncertainty for final test results can be obtained. The procedures are not applicable to methods other than enumeration methods ...
A preliminary experiment was carried out to study the effect of Agnihotra on the bacterial population in a room where Agnihotra was performed. For this study, two rooms of equal dimensions (134 x 8 x 11) were selected. In both the rooms fire was prepared from dried cowdung cakes in copper pyramids and the basal reading of number of microorganisms in both the rooms was taken by exposing blood agar plates at four corners of the room for 10 minutes. This was done exactly half an hour before Agnihotra time. Agnihotra was performed exactly at sunset in one of the rooms.. Bacterial counts were taken again in both the rooms in a similar manner at half hour intervals. Thus readings were taken in both the rooms up to two hours after performance of Agnihotra. It was quite interesting to note that microbial counts in the room where Agnihotra was performed were reduced by 91.4% (Figs. 1a, 1b and 1c illustrate the reduction in the number of microorganisms) whereas the room where only fire was generated ...
Determining E. coli levels in sediments and its ability to attach to sand and silt and float downstream will help scientists figure out what needs to be done to decrease bacterial levels in streams.
Spontaneous bacterial colonization by CONS in Mgb-/- females versus Mgb+/- and WT controls.Bacteria recovered from urine (CFU/ml), bladders, and kidneys (CFU/or
Sushi and sashimi are traditional Japanese food, mostly consisting of raw seafood alone or in combination with rice. Eating sushi and sashimi has become popular in many countries even outside Japan. This food is not free from health risks such as ingestion of pathogenic bacteria or parasite. The aim of this study was to investigate on hygienic-sanitary quality of sushi and sashimi sold in the cities of Messina and Catania, Southern Italy. Fifty samples (38 sushi and 12 sashimi) were analysed to determinate the aerobic mesophilic bacteria (AMB), psycrophilic bacteria (PB), Enterobacteriaceae, specific spoilage organisms (SSOs), Pseudomonas spp., coagulase-positive staphylococci, micrococci, Vibrio spp., Bacillus cereus, Salmonella spp. and Listeria monocytogenes. In sushi, AMB ranged from 5.00 to 8.18 log CFU/g, PB from 4.70 to 7.13 log CFU/g, Enterobacteriaceae from 1.41 to 6.67 log CFU/g, while SSOs and Pseudomonas spp. from 3.49 to 7.72 and from 3.36 to 8.00 log CFU/g, respectively. Micrococci ...
Total Bacterial Count and Presence of Escherichia coli in Raw Ground Beef Samples Obtained From Three Major Supermarkets on the Island of Bonaire.
KAUME, Lydia; FOOTE, Jerald C. y GBUR, Edward E.. Microbial contamination of herbs marketed to HIV-infected people in Nairobi (Kenya). S. Afr. j. sci. [online]. 2012, vol.108, n.9-10, pp.1-4. ISSN 1996-7489.. Herbal products are used by human immunodeficiency virus (HlV)-infected individuals regardless of safety or efficacy concerns. In this study, we examined the microbiological quality of herbal preparations marketed to HIV-infected individuals. A convenience sample (N = 24) of herbal products was obtained from retailers in Nairobi, Kenya in 2007. Petrifilm plate count methods were used to estimate total aerobic bacteria (APC), coliform, Escherichia coli, Staphylococcus aureus and yeast and mould counts. APC counts ranged from an estimated 1.5 x 101 colony forming units (CFU)/g to 7.1 x 108 CFU/g. Total and faecal coliform counts ranged from an estimated ,10 CFU/g to 3 x 106 CFU/g. E. coli load ranged from ,10 CFU/g to 5 x 101 CFU/g and S. aureus counts ranged from an estimated ,10 CFU/g to ...
Myeloperoxidase (MPO) is an enzyme that plays a central role in mammalian antimicrobial defense, including eliminating bacteria from skin wounds. Staphylococcal species are estimated to affect more than 70% of human skin wounds due to their high prevalence and resistance to antimicrobial treatments. This research was conducted to investigate the ability of an MPO-containing formulation to combat Staphylococcus aureus infection.. A porcine deep partial-thickness wound model was used in the study. Wounds were inoculated with S aureus and then treated with high- or low-concentration MPO formulations. Wounds also were treated with placebo, saline, and mupirocin, which served as a positive control, and untreated wounds served as a negative control. Treatment with the MPO formulations reduced the challenge organisms by 3 log CFU/mL compared with the 8 log CFU/mL recovered from the untreated, placebo, and saline groups (P ,.01). These results indicate that MPO-based drug products may be beneficial in ...
SummaryThis purpose of this experiment was for students to do the colony count methods, estimating the viable cell number of commercial active dried yeasts (ADY).This experiment allowed the students to perform the plate count technique by serial diluti...
A total of 154 samples of sweet dumpling food(SDF), including 102 samples of the flour-packed SDF and 52 samples of the rice-packed SDF, were bought from the conventional markets, supermarkets, confectioneries, and temples throughout Taiwan. The microbiological quality was assessed by the assay of aerobic viable cell count, coliform, Escherichia coli, Bacillus cereus, and related pathogens. The physical properties including pH value, water activity(Aw), and Brix of SDF were also tested. A comparison of the flour-packed SDF with the rice-packed SDF showed significant differences in the detection of aerobic viable cell count and coliform. Incidence of E. coli was 9.80% in the flour-packed SDF and 11.53% in the rice-packed SDF. B. cereus was identified exclusively in the flour-packed SDF. Two strains of newly ascribed food poisons pathogens, B. subtilis and B. pumilus, were isolated. Among them, B. subtilis was detected in three samples of the rice-packed SDF and in one of the flour-packed SDF, while B.
Bacterial populations in some organs, viz., liver, spleen, kidney, gill, and arborescent organ of the catfish Clarias batrachus were enumerated followed by determination of resistance for antibiotics and metals. The total viable counts in these organs, observed, were 2.24x10(4), 2.08x10(4), 1.44x10(4), 1.23x10(4), and 6.40x10(3) colony-forming units/mL, respectively. The random bacterial isolates from these fish organs showed resistance in decreasing order for colistin (98%), ampicillin (82%), gentamycin (34%), carbenicillin (28%), tetracyline (20%), streptomycin (12%), and ciprofloxacin (02%). Most of the isolates exhibited an increasing order of tolerance for the metals (microg/mL) copper (100), lead (200), manganese (400), cadmium (200), and chromium (50), with minimum inhibitory concentration (MIC) ranging from |50 to 1600 microg/mL. These observations indicate that the significant occurrence of bacterial population in organs of fish with high incidence of resistance for antibiotics and metals may
BioLumix is the most advanced microbiological testing system of its kind. This automated, all-in-one microbial testing system is extremely easy to operate. The system is both simple and cost-effective, revolutionizing your current testing methodology. A novel optical system sensing color and fluorescence in ready-to-use vials provides faster results, labor savings, automation, and connectivity. The system has a large repertoire of assays that it can perform including: Total Aerobic Count, Yeast and Mold, Coliforms, Enterobacteriaceae, E. coli, Pseudomonas, Staphylococcus, and Salmonella.. Recently a new assay for the detection of Heterotrophic bacteria in water was developed for the BioLumix system. The developed assay was validated by testing 50 samples of multiple types of water that were tested by the BioLumix method and the plate count method side-by-side. The BioLumix vials were directly inoculated with 0.1 mL of the water sample, or 1.0 mL of a 1:100 dilution (depending on the desired ...
The importance of the viable but nonculturable (VBNC) state, with respect to safety of environmental applications of genetically engineered microorganisms (GEMs), can best be appreciated in risk...
Wrap the agar strip holder with aluminium foil and autoclave at 121°C (15 lbs) for 15 minutes. Carry the Dyna test unit to the test location. Carefully insert the pre sterilized agar strip into the holder and assemble the unit. Load the cells or connect the power point. Keep the timer switch at position one, sample the air for one minute. Focus the agar strip holder towards the area which is to be tested. Switch on the unit. After one minute the instrument will shut off automatically. Remove the agar strip from the holder and keep in the case provided and incubate at desired temperature for 48-72 hours. Report the result as c.f.u/40 L of air samples. The instrument can draw 40 L of air on to the agar strip per minute ...
When the companys fully automated GreenLight® 930 is used, the vials can be batch loaded onto the 48-position carousel, or individually loaded at any time during the units continuous mode. As bacteria in the test sample multiply and respire, they consume oxygen. The change in oxygen is used to calculate the milks colony forming units per milliliter. (Bacterial load is calculated in the PC and stored to a secure data base ...
An apparatus for automatically transferring a desired one of bacterial colonies grown in a medium in a culture Petri dish onto a medium in a test Petri dish or a test tube by using a bacterial colony pick-up/transfer element of the disposable type or a bacterial colony pick-up/transfer element of the reuse type and by using various mechanisms controlled by a computer.
Field studies conducted on three separate well pads showed that one 2K7 Water Soluble Pak (WSP) per 10 m3 (63 bbl) surface water effectively reduced bacterial levels from ,106 to,104 with agitation of the fluid reducing the reaction time. This fluid was then used for CT bridge plug milling operations and bacteria levels monitored at various fluid locations in the closed loop throughout the operations.. Evaluation of flowback from the wellbore indicated consistently lower bacterial concentrations than the injected surface water. Despite this, bacteria levels at surface were rebounding. Therefore, the observed increases in surface bacteria were due to the population rebounding while sitting in the surface tanks.. This rebound effect was because the CT fluid for the mill-out operations was reused for each successive pad well. As flowback water was introduced into the surface tanks, so were the additives (polymers, surfactants) from stimulation operations that, along with dissolved formation ...
Field studies conducted on three separate well pads showed that one 2K7 Water Soluble Pak (WSP) per 10 m3 (63 bbl) surface water effectively reduced bacterial levels from ,106 to,104 with agitation of the fluid reducing the reaction time. This fluid was then used for CT bridge plug milling operations and bacteria levels monitored at various fluid locations in the closed loop throughout the operations.. Evaluation of flowback from the wellbore indicated consistently lower bacterial concentrations than the injected surface water. Despite this, bacteria levels at surface were rebounding. Therefore, the observed increases in surface bacteria were due to the population rebounding while sitting in the surface tanks.. This rebound effect was because the CT fluid for the mill-out operations was reused for each successive pad well. As flowback water was introduced into the surface tanks, so were the additives (polymers, surfactants) from stimulation operations that, along with dissolved formation ...
1) Food poisoning The saprophytic bacteria cause decay of our food and make it unpalatable. Toxins are produced as a result of bacterial growth on the foodstuffs. Due to this, food poisoning occurs.
I am looking for a table listing the normal concentrations or concentration range of typical cell metabolites, e.g., amino acids, NAD, ATP, ions, sugars, major proteins, etc. A few years back I once stumbled over such a table. Unfortunately, I did not make a copy. The only entries I can remember: typical cell protein concentration 100-300mg/mL acetate ion 0.2 - 0.25 M Does anybody know where to find such a table ? I am also looking for a database, printed, on CD or diskette, or on the Net, with physiological data; e.g., average yield for fermentative bacterial growth on glucose 10.5g cells / mol ATP; list of average generation times of different bacteria; metabolic specialties; and so on. Any help would be appreciated. Achim ...
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The primary benefit of betain HCL is its effect on stomach acid production It may also help to manage bacterial levels in the stomach, facilitate digestion and support the absorption of nutrients
M. avium CFU counts are higher in lung tissue of infected HO-1 -/- mice as compared to lung tissue form infected HO-1+/+ miceMouse lung tissue was homogenized i
Aerobic microorganisms. Determination in foods at 37 °C, 30 °C, 25 °C, 20 °C, 17/7 °C or 6.5 °C by the colony count method. (NMKL 86, 5. Ed., 2013 ...
Each cell will undergo multiple rounds of cell divisons to produce separate colonies on the plate, so each cell is a colony forming unit. After incubation the number of colonies will reflect the number of CFUs originally present. This test gives the viable count (living cells only) compared to a microscopic count or dry weight test that gives the total cell count (living and dead cells ...
I am suffering from Chronic Lympomatic Lukemia. Initially in 2008 I was diagnosed with high Total count of 33000 when I was pregnant. Me and my husband consulted various doctors and conducted various scans . At that time Doctors advised that this high total count could be because of Pregnancy. And after delivering my child my count became normal and was 13000. ...
Early-season dollar spot control was mentioned in a previous post by Dr. Kaminski. Basically fungicides are applied long before symptoms develop in the field, which results in a significant delay in the onset of symptoms. The proposed theory behind early-season applications is they reduce the pool of initial inoculum of the dollar spot fungus enough […]. ...
Is it okay if I just ignore the cfu and use the value as millimol? Will it remain same? I just want the concentration of ATP inside the bacterial cell ...
The easiest solution would be to add timestamps to logs, or log to different logs from oddjob or from installer (ipareplica-conncheck.local.log and ipareplica-conncheck.remote.log) Actually the easiest solution would be not to log into a file when executed from oddjob ...
Comparative evaluation of subgingivally delivered chlorhexidine varnish and chlorhexidine gel in reducing microbial count after mechanical periodontal therapy, Sathish Manthena, Am
The number of bacteria tested by heterotrophic plate count method showed a very high content from the daily used reusable drinking water bottles. It is in the range from 0 to 2.4x105 CFU/mL with an average about 34,000 bacteria counts for bottles used by children and about 75,000 bacteria counts for bottles used by adults (Figures 2 and 4). The difference between the two is expected due to the bottle design since most bottles used by children have straws, and there is less chance of direct contact between the water in the bottle and the mouth of the users or other possible bacteria contamination sources. This may lead to a lower bacteria number in the bottles. Among all the sixty bottles sampled, there is only one bottle (2%) with bacteria number less than 500 CFU/mL. The bacteria number is much higher than those detected in the bottled drinking water which is in the range of 2-150 CFU/ml [10], but it has a similar level to the bottled natural mineral water after storing for a few days. Gonzalez ...
Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC) retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore®) is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM) was comparable to plate count method when the spores were counted at
The formation of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) transcriptomics and isobaric tag for relative and absolute quantitation (iTRAQ) proteomic methods. The analyses revealed that 97 genes and 56 proteins were significantly changed upon VBNC state entry. Genes and proteins related to membrane transport, central metabolisms, DNA replication, and cell division were mainly downregulated in the VBNC cells. This caused low metabolic activity concurrently with a division arrest in cells, which may be related to VBNC state formation. Cell division repression and outer membrane overexpression were confirmed to be involved in VBNC state formation by homologous expression of z2046 coding for transcriptional repressor and ompF encoding outer membrane protein F. Upon VBNC state entry, pyruvate catabolism in the cells shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route; this led ...
Hydrocarbon utilizing bacteria were isolated from diesel impacted sites at the University of Port Harcourt using the vapour phase transfer method. The isolates were identified as Bacillus, Pseudomonas, Acinetobacter, Serratia, and Micrococcus species. Examination of cultures supplemented with glucose revealed that the total viable counts ranged from 1.7 x 104 to 5.9 x 105 cfu/ml. For cultures supplemented with kerosene the total viable counts ranged from 1.18 x 104 to 3.1 x 105 cfu/ml. Bacillus spp. and Micrococcus spp. gave the highest and lowest counts respectively in both media types within 96 h. The other isolates had counts in between these ranges in the respective supplemented media. The growth of the isolates in mineral salts solution supplemented with glucose or kerosene resulted in turbidity of the broth as compared to clear media solutions in controls. Higher turbidity was recorded in media supplemented with glucose than kerosene, and this was reflected in the total viable counts ...
Interpretive Summary: Technical Abstract: Fast pyrolysis of switchgrass (and resultant biochar) can be used for bio-fuel production, soil amendments for fertilizing crops, binding heavy metals, and sequestering environmental biocarbon. To determine the influence of fast pyrolysis-generated switchgrass biochar on survival of foodborne pathogens and potential surrogate bacteria in soil. Soil was amended with 7.5% biochar and inoculated with one of the following seven bacterial composites: E. coli O157:H7 (EHEC), Salmonella enterica, non-O157 Shiga toxin E. coli (STEC), ATCC nonpathogenic E. coli (BSL-1), attenuated EHEC-A, ATCC-attenuated EHEC-B, and attenuated Salmonella at 6.9 - 8.0 log CFU/g of soil, 6.9 percent moisture, sealed, and stored at 22 degrees Celcius. Twenty-four hours after inoculation, all biochar-amended soil populations were 0.33 - 1.40 log CFU/g lower than biochar-free soils, except for STEC, which declined by 1.51 log CFU in both soils. By day 5, populations of STEC and EHEC-A ...
Dr. Clifford Obi*. ABSTRACT. Microbiological and proximate analyses of 50 Home and Industrial Made soymilk samples were carried out using standard analytical procedures. The two soymilk types were contaminated with Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa with E. coli having the highest occurrence level (42%) and Klebsiella pneumoniae being the lowest (8%). Two fungi: Aspergillus niger and Penicilium notatum were also recovered from the soymilk samples with A. niger being higher in occurrence (66.7%) than P. notatum (33.3%). The total viable bacterial count (TVBC) for the Home made soymilk was in the range 6.2 x 106 - 4.0 x 105 CFU/ml while the TVBC for the Industry made was in the range 2.0 x102 - 1.0 x 102 CFU/ml. The total fungal count (TFC) for the Home made soymilk was in the range 4.1 x 106-3.0 x 105 CFU/ml while the TFC for the Industry Made was in the range 2 x 102-1.0 x 102 CFU/ml. Antibiotic sensitivity screening result showed that the ...
Reducing microbial contamination in wheat is desirable to ensure consumer safety. The purpose of this study was to determine the efficacy of adding organic acids and NaCl to tempering water to reduce microbial load in hard wheat prior to milling, and to determine the impact that these treatments might have on the microbial quality and functional properties of straight-grade and whole grain flours. Wheat was tempered to 15.5% moisture under controlled (24h, 73-75°F, 60% RH), aseptic conditions by adding water (control) or tempering solutions containing acid (acetic or lactic; 2.5% and 5% v/v) and NaCl (26% w/v). Wheat was analyzed before and after tempering for Total Plate Counts (TPC), yeasts, molds, and Enterobacteriaceae (Eb). The microbial load of the tempered wheat was significantly reduced by all organic acid-NaCl treatments (p,0.05). The combination of lactic acid (5%) and NaCl was the most effective against TPC and Eb (p,0.05), with an average reduction of 3.5 and 4.7 log CFU/g, ...
Our Microbiology division offers a wide range of microbiological analysis of environmental water samples as well as antimicrobial tests for consumable products, such as Heterotrophic plate count, Total coliforms, Faceal coliforms, E. coli and Legionella.
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293335144 - EP 1040841 A1 2000-10-04 - Anticoagulant/sterilizing compositions and methods - Compositions and methods are provided for preventing formation of thrombosis and/or bacterial growth on a liquid-contacting surface of a liquid delivery system, such as a port, catheter or port-catheter system. The liquid delivery system is connected to a patient for delivery of a liquid to the patient. The method involves contacting the surface with a thrombosis-preventing liquid containing taurolidine, taurultam or a mixture thereof, the thrombosis-preventing liquid further containing an anticoagulant agent. In an alternative embodiment, the liquid-contacting surface of the delivery system is contacted with a solution containing an anticoagulant agent, and thereafter contacted with a solution containing taurolidine, taurultam or a mixture thereof.[origin: EP1040841A1] Compositions and methods are provided for preventing formation of thrombosis and/or bacterial growth on a liquid-contacting surface of a liquid
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PILONETTO, Marcelo et al. Hospital gowns as a vehicle for bacterial dissemination in an intensive care unit. Braz J Infect Dis [online]. 2004, vol.8, n.3, pp.206-210. ISSN 1413-8670. http://dx.doi.org/10.1590/S1413-86702004000300003.. The microbiota from the uniforms of 31 professionals from the general intensive care unit was analyzed. The samples were collected in duplicate at the beginning and at the end of the work period. Total viable counts of microorganisms were determined; there was a significant increase in the counts at the end of the period, when compared with those obtained at the beginning. No significant difference was observed between the first and second counts obtained from the cuffs. However, differences were observed for the samples from the abdominal region. Among the isolated pathogens 11/18 were Staphylococcus aureus, 2/18 were Acinetobacter baumannii, 2/18 were Klebsiela pneumoniae and 1/18 were Serratia rubidae. Some of these isolates were multi-resistant to antibiotics. ...
Interpretive Summary: Technical Abstract: The need for a nonthermal intervention technology that can achieve microbial safety without altering nutritional quality of liquid foods led to the development of the radio frequency electric fields (RFEF) process. Previously, we documented formation of surface blebs on Escherichia coli cells treated with RFEF. In this study, we used transmission electron microscopy (TEM) to investigate the effects of heat and RFEF treatments on E.coli K-12 cell membrane. Apple juice purchased from a wholesale distributor was inoculated with Escherichia coli K-12 at 7.8 log CFU/ml and then treated with heat (75 deg C, 5 min) or with RFEF operated at 55C and 25 kV/cm for 3.4 milliseconds at a flow rate of 540 ml/min. Treated samples were monitored for changes in cell membrane using Transmission Electron Microscopy (TEM) while intracellular leakage of biological active compounds was determined using bioluminescence ATP assay. Surviving cell populations was determined by ...
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The viable cell count (Table 4-1) is typically considered the measure of cell concentration. For this, a 1-mL volume is removed from a bacterial suspension and serially diluted 10-fold followed by plating 0.1-mL aliquots on an agar medium. Each single invisible bacterium (or clump of bacteria) will grow into a visible colony that can be counted (see Chapter 5). For statistical purposes, plates containing between 30 and 300 colonies give the most accurate data. The plate count × the dilution × 10 will give the number of colony forming units (CFU)/mL in the undiluted bacterial suspension. Using this method, dead bacteria within the suspension do not contribute to the final bacterial count. ...
* found in: m-ColiBlue24® Broth, EC Medium with Mug, EC Medium MPN Tubes PK/15, Modified Colitag 16-HR, Peel Plate® Heterotrophic Plate Count Media, Use m..
The volume retrieved is ∼0.001 mL (range 0.01-0.001) of lower respiratory secretions and dilution into the holding medium increases the dilution of the culture plate by 100-1,000-folds. Quantitative bacterial cultures of PSB allow the distinction between colonization and infection. Quantitative cultures represent serial dilutions of the respiratory samples. The colony counts are calculated by the number of colonies visible on the agar plate in relation to the dilution and inoculation factors. The cut-offs for quantitative culture results were established by relating colony counts known to be present in sputum samples of pneumonia patients to the estimated amount of respiratory secretions retrieved by the technique. Thus, it should be noted that the quantitative culture technique is based on rough inferences rather than exact measurements. For PSB, the currently accepted threshold is 103 cfu·mL−1. Growth ≥103 cfu·mL−1 is considered significant for infection (table 1⇑) and corresponds ...
THEMATIC ISSUE ON Viable But Non-Culturable Microbes. Editors: Wolfgang Kneifel, Clemens Kittinger. The scope of this issue will focus on the so called viable but non- ...
Lebanon bologna raw batter was mixed with a five-strain mixture of Escherichia coli O157:H7 to achieve average inoculum levels of 7.79, 7.77, and 7.92 log CFU/g as deter mined on MSA, 202, and PRSA media, respectively ...
Chromogenic/TTC Dipslide Tests for E.coli, Pseudomonas Aeruginosa and Total Viable Count for enumeration of E.coli. Flexible paddle for effective surface contact ...
Characterization of Culturable Bacteria in the Atmospheric Environment in Incheon, Korea - Atmospheric environment;Total plate counts;
Food poisoning and infection by bacteria are of public health significance to both developing and developed countries. Samples of ogi (akamu) prepared from white and yellow variety of maize sold in Uturu and Okigwe were analyzed together with the laboratory prepared ogi for bacterial quality using the standard microbiological methods. The analyses showed that both white and yellow variety had total bacterial counts (cfu/g) of 4.0 ×107 and 3.9 x 107 for the laboratory prepared ogi while the commercial ogi had 5.2 x 107 and 4.9 x107, 4.9 x107 and 4.5 x107, 5.4 x107 and 5.0 x107 for Eke-Okigwe, Up-gate and Nkwo-Achara market respectively. The Staphylococcal counts ranged from 2.0 x 102 to 5.0 x102 and 1.0 x 102 to 4.0 x102 for the white and yellow variety from the different markets while Staphylococcal growth was not recorded on the laboratory prepared ogi. The laboratory prepared ogi had no Coliform growth while the commercially prepared ogi had counts of 0.5 x103 to 1.6 x 103 for white variety ...
Air sampling during the incision of 40 septic lesions did not produce any evidence of dispersal of pathogenic bacteria. The removal of dressings in a sterile dressing-box was associated with the dispersal of both pathogenic and non-pathogenic bacteria. From 45 dressings the average total bacterial count was 104 bacteria-containing particles per cubic foot. Thirteen out of 26 staphylococcal lesions dispersed staphylococci when the dressings were removed, the levels of staphylococci ranging from 0.3 to 33.3 colonies per cubic foot.
Boost productivity and cut lab costs with our award-winning 3M™ Petrifilm™ plates. Get microbial counts for E. coli and other coliform bacteria within 18-24 hours.
摘要(Abstract): [目的]探究制药废水中活的但非可培养(viable but non-culturable,VBNC)状态细菌的组成与系统关系。[方法]利用土质滤材构建生物反应器,基于复苏促进因子(resuscitation promoting factor,Rpf)对VBNC细菌的复苏刺激生长作用,采用MPN(most probable number)法,稀释平板法分离其中复苏可培养化的VBNC菌株并解析其16S ...
Antibiotics kill bacteria or cause them to be unable to reproduce. One of the effects of antibiotics on bacterial count is a decrease in the growth of the bacteria. Sample collection prior to beginning antibiotic treatment is essential, for accurate bacterial counts.
The average microbial load of bacteria isolates from the samples ranges between 3.0 -4.7 x 104 cfu/ml., for fungal isolates it ranges between 1.0x104 to 2.9x104 cfu/ml and yeast counts from 0.0 x104 cfu/ml in fura to 5.3 x104 cfu/ml in fura da nono ...
In order to identify and quantify the microorganisms present in a certain ecosystem, it has become necessary to develop molecular methods avoiding cultivation, which allows to characterize only the co
Perfumed with mint, this natural enzymes based spray helps to reduce the bacterial proliferation in your dogs mouth and limits tartar formation on teeth. NON TOXIC, can be safely ingested by your pet. Available in 125 ml tube. ...
Repairing and soothing nourishing ointment. Indications: For damaged skin (small lesions). Product benefits: Restores the epidermis and moisturises Relieves discomfort and reduces the urge to scratch Prevents bacterial proliferation How to use: Twice daily until your skin is fully repaired.
Harmless, but very powerful radicals will decrease the microbial load and air particles in your company and disinfect your room complete.
MeshCellCount[mreg] gives a list {c0, c1, ...} of counts cd of cells of dimension d in the mesh region mreg. MeshCellCount[mreg, d] gives the total count of cells of dimension d. MeshCellCount[mreg, cellspec] gives the total count of cells specified by cellspec.
I am new to xsd, and found out that the xsd:enumeration value containing colon is not getting validated.Heres a brief of the value I am trying to include ...
The way to maintain a tree structured enumeration while having all the advantages of the standard ones; Author: Smart K8; Updated: 2 Apr 2008; Section: C#; Chapter: Languages; Updated: 2 Apr 2008
The way to maintain a tree structured enumeration while having all the advantages of the standard ones; Author: Smart K8; Updated: 2 Apr 2008; Section: C#; Chapter: Languages; Updated: 2 Apr 2008
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