PURPOSE: To evaluate the fluoroquinolone susceptibilities of ocular isolate coagulase-negative staphylococci (CoNS), identified at the Microbiology Laboratory - UNIFESP. DESIGN: Experimental laboratory investigation. METHODS: The minimum inhibitory concentrations (MICs) of 21 strains of methicillin-resistant coagulase-negative staphylococci (MRCoNS) and 22 methicillin-sensitive coagulase-negative staphylococci (MSCoNS) to ciprofloxacin, ofloxacin, gatifloxacin and moxifloxacin were determined, using the E-test method standardized by the Clinical and Laboratory Standards Institute (CLSI/NCCLS). RESULTS: The MIC90s (µg/ml) for the second generation of tested fluoroquinolones were higher than the fourth generation, especially for the methicillin-resistant coagulase-negative staphylococci group. CONCLUSION: Our results indicate that methicillin-sensitive coagulase-negative staphylococci are more susceptible to quinolones than are methicillin-resistant coagulase-negative staphylococci and that ...
References for Abcams Anti-Staphylococcus aureus coagulase Type I antibody (ab79636). Please let us know if you have used this product in your publication
The pathogenic role of staphylococcal coagulase and clumping factor was investigated in the rat model of endocarditis. The coagulase-producing and clumping factor-producing parent strain Staphylococcus aureus Newman and a series of mutants defective in either coagulase, clumping factor, or both were tested for their ability (i) to attach in vitro to either rat fibrinogen or platelet-fibrin clots and (ii) to produce endocarditis in rats with catheter-induced aortic vegetations. In vitro, the clumping factor-defective mutants were up to 100 times less able than the wild type strain to attach to fibrinogen and also significantly less adherent than the parents to platelet-fibrin clots. Coagulase-defective mutants, in contrast, were not altered in their in vitro adherence phenotype. The rate of in vivo infection was inoculum dependent. Clumping factor-defective mutants produced ca. 50% less endocarditis than the parent organisms when injected at inoculum sizes infecting, respectively, 40 and 80% ...
Antimicrobial resistance patterns and gene coding for methicillin resistance (mecA) were determined in 25 S. aureus and 75 Coagulase Negative Staphylococci (CNS) strains isolates from half-udder milk samples collected from goats with subclinical mastitis. Fourteen (56.0%) S. aureus and thirty-one (41.3%) CNS isolates were resistant to one or more antimicrobial agents. S. aureus showed the highest resistance rate against kanamycin (28.0%), oxytetracycline (16.0%), and ampicillin (12.0%). The CNS tested were more frequently resistant to ampicillin (36.0%) and kanamycin (6.7%). Multiple antimicrobial resistance was observed in eight isolates, and one Staphylococcus epidermidis was found to be resistant to six antibiotics. The mecA gene was not found in any of the tested isolates. Single resistance against β-lactamics or aminoglicosides is the most common trait observed while multiresistance is less frequent. ...
Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive. It is also produced by Yersinia pestis. Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen, a plasma protein produced by the liver, to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. The fibrin clot may protect the bacterium from phagocytosis and isolate it from other defenses of the host.The fibrin coat can therefore make the bacteria more virulent. Bound coagulase is ...
Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is coagulase-positive, meaning that coagulase negativity excludes S. aureus.. It is also produced by Yersinia pestis.. Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. It has been proposed that fibrin-coated staphylococci resist phagocytosis, making the bacteria more virulent. Bound coagulase is part of the larger family of MSCRAMM. ...
Author: Surekha.Y.Asangi , Mariraj.J , Sathyanarayan.M.S , Nagabhushan , Rashmi :: Coagulase Negative Staphylococci (cons) Are The Indigenous Flora Of The Human Skin And Mucous Membrane. They Are Usually Contaminants, When Isolated From A Clinical Specimen. These Organisms Are Becoming Increasingly Recognized As Agents Of Clinically...
BACKGROUND: Antimicrobial resistance may compromise the efficacy of antibiotic prophylaxis before surgery. The aim of this study was to measure susceptibility and clonal distribution of coagulase-negative staphylococci (CoNS) colonizing the skin around the surgery access site before and after the procedure. METHODS: From March to September 2004, a series of 140 patients undergoing elective major abdominal surgery were screened for CoNS colonization at admission and 5 days after surgery. All isolates were tested for antibiotic susceptibility and genotyped by pulsed-field gel electrophoresis (PFGE). RESULTS: Colonization rates with CoNS at admission and after surgery were 85% and 55%, respectively. The methicillin-resistant CoNS rate increased from 20% at admission to 47% after surgery (P = 0.001). The PFGE pattern after surgery revealed more patients colonized with identical clones: 8/140 patients (8/119 strains) and 26/140 patients (26/77 strains), respectively (P , 0.001). CONCLUSIONS: Our ...
Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim of this study was to characterize CoNS with reduced susceptibility to glycopeptides causing blood stream infection (BSI) in critically ill and haematology patients at the University Hospital Tor Vergata, Rome, Italy, in 2007. Hospital microbiology records for transplant haematology and ICU were reviewed to identify CoNS with elevated MICs for glycopeptides, and isolates were matched to clinical records to determine whether the isolates caused a BSI. The isolates were tested for susceptibility to new drugs daptomicin and tigecycline and the genetic relationship was assessed using f-AFLP. Of a total of 17,418 blood cultures, 1,609 were positive for CoNS and of these, 87 (5.4%) displayed reduced susceptibility to
Legionella species are generally susceptible to fluroquinolones or macrolides "Small-colony variant" Staphylococcus aureus (SCV) These are a form of Staphylococcus aureus. They are not recognised on routine culture media as they grow slowly. They also have a delayed coagulase positive test which can be delayed up to 24 hours. The common (or the wild type) S aureus compared takes just 4 hours to exhibit coagulase positivity. For this reason, these are commonly identified as coagulase-negative staphylococci. The best way to recognise them is to incubate for at least 3 days and look for pinpoint colonies, and then to await the coagulase positive test. These microbes have a reduced metabolic capacity and are therefore less pathogenic. However, it can persist intracellularly and cause chronic infection, and are resistant to routine antibiotics (which are effective against the wild type). They are very common in patients who have received or are on antibiotics, especially aminoglycosides. SSIs caused ...
One of the most important phenotypical features used in the classification of staphylococci is their ability to produce coagulase, an enzyme that causes blood clot formation. Seven species are currently recognised as being coagulase-positive: S. aureus, S. delphini, S. hyicus, S. intermedius, S. lutrae, S. pseudintermedius, and S. schleiferi subsp. coagulans. These species belong to two separate groups - the S. aureus (S. aureus alone) group and the S. hyicus-intermedius group (the remaining five). An eighth species has also been described - Staphylococcus leei - from patients with gastritis.[17]. S. aureus is coagulase-positive, meaning it produces coagulase. However, while the majority of S. aureus strains are coagulase-positive, some may be atypical in that they do not produce coagulase. S. aureus is catalase-positive (meaning that it can produce the enzyme catalase) and able to convert hydrogen peroxide (H2O2) to water and oxygen, which makes the catalase test useful to distinguish ...
Hello Eric, Streptococcus genus contains both beta-hemolytic and alpha hemolytic species. S. pyogenes and S.agalactiae are beta-hemolytic but S.pneumoniae and viridans streptococci (common name for various Streptococci species that gives alpha hemolysis) are alpha hemolytic. In Staphylococci, S.aureus is the pathogenic one but S. epidermidis, S. saprophyticus are mostly commensal (normal flora) and occasionally be pathogenic. So common phrase Coagulase Negative Staphylococci (CONS) is used for them; (S. aureus is coagulase positive).. ...
From a total of 40 characters that were previously used to differentiate species of staphylococci, 13 key characters were selected to make a simplified scheme that could be easily used by the routine clinical laboratory for identifying human staphylococci. These key characters included coagulase activity, hemolysis, nitrate reduction, and aerobic acid production from fructose, xylose, arabinose, ribose, maltose, lactose, sucrose, trehalose, mannitol, and xylitol. In the simplified scheme, 924 strains of staphylococci were placed into 11 positions, each of which contained the major portion (greater than or equal to 80%) of strains of one of the recognized species. Several positions contained a rare or few uncommon strains of one or more additional species and these could be resolved on the basis of other key characters. ...
Results: The incidence of NIs was low (i.e. , 2%). Among 16936 admitted patients in this hospital, 174 patients (79 males and 95 females) with a mean age of 51.7 ± 24.6 years (range, 5 to 90 years) were diagnosed with an NI. Incidence density of NIs were 3.18% in infectious diseases ward, 2.17% in intensive care unit (ICU), 2% in orthopedic ward, 0.68% in obstetrics and gynecology (OBGYN) ward and 0.278% in general surgery. Regarding the etiology of infection, coagulase-negative staphylococci in 23.69%, Bacillus in 21.05%, Escherichia coli was found in 18.42%, and coagulase-positive staphylococci in 13.16% of the cases. The results indicated that coagulase-negative staphylococci was the most frequent pathogen. ...
Lyophilized rabbit plasma, for the identification of Staphylococcus aureus|/italic by the coagulase testDehydrated microbiology culture media cultivate and isolate microorganisms for researching purposes. Fast-dissolving powders come premixed for quick preparation using neutral liquids. By standardizing the media employed, the low-dust granule mixtures guarantee that the correct enrichment will be present for optimal cell viability and growth. The mixtures have extensive dry storage capabilities. The vast ingredient selection ensures that the most compatible dehydrated microbiology culture media will be available.
常見的臨床微生物簡介. 萬芳醫院 感染科 歐聰億. 格蘭氏染色 and 形狀. 格蘭氏陽性. 葡萄球菌- Staphylococcus Staphylococcus aureus Staphylococcus epidermidis Coagulase negative staphylococcus (CoNS). 鏈球菌 - streptococcus Streptococcus pneumoniae (pneumococcus) Viridans streptococcus (αhemolytyic)( 綠色) Slideshow 6870346 by lisandra-graham
Merlino, J., Kay, I., Coombs, G., Palladino, S. (2010). PCR Assays in Detecting Methicillin Resistance in Staphylococci: Coagulase Negative Staphylococci (CNS), S. Aureus, and MRSA with the PVL Gene. In Margaret Schuller, Theo Sloots, Gregory James, Catriona Halliday, Ian Carter (Eds.), PCR for Clinical Microbiology - An Australian and International Perspective, (pp. 407-414). Dordrecht: Springer Science+Business Media. [More Information] ...
Oliveira, Ad lia Dias Dourado et al. Laboratory detection methods for methicillin resistance in coagulase negative Staphylococcus isolated from ophthalmic infections. Arq. Bras. Oftalmol., Aug 2007, vol.70, no.4, p.667-675. ISSN 0004- ...
Staphylococcus is a gram positive, cocci shaped, genus of bacteria. Observed under a microscope will reveal they exist in microscopic grape-like clusters. One species of staphylococci, Staphylococcus aureus ...
Central venous catheter represents a major source of nosocomial bloodstream infection, which cause considerable excess morbidity.The diagnosis of catheter-related infections relies on the presence of clinical manifestation of infection and the evidence of colonization of the catheter tip by bacteria or fungi. The most frequent pathogens were Gram-positive organisms, mainly coagulase negative staphylococci, followed by Gram-negative and mycetes. During the years 2001-2003 we examined 2079 vascular catheters, of which 896 were positive for bacterial and fungal species. Of these, 675 (75,3%) involved Gram-positive bacterial, 145 (16,2%) Gram-negative and 76 (8,5%) mycetes ...
Manufacture and distribution of animal blood products, plasma, serum, microbiological diagnostic kits and quality control products, Buckinghamshire, UK
But Im a little different to him, Im called a "coagulase negative" staph, which means I react differently then him in a special test. ...
But Im a little different to him, Im called a "coagulase negative" staph, which means I react differently then him in a special test. ...
Skin microbiome primary cultivable aerobes in human are coagulase-negative staphylococci and lipophilic corynebacteria. in the research of human physiological flora which has been recently called microbiome (Turnbaugh et al. 2007). Natural flora plays an important role in a process of preventing colonization of the skin by pathogenic organisms known as colonization resistance of the skin. […]. Read More ». ...
Items covered in a dichotomous key include whether bacteria are Gram positive or negative, catalase positive or negative, have nitrate enzymes, and contain the enzyme coagulase. The key is a set of...
Find out if a staph infection is contagious, learn how a staph infection is transmitted, and discover when to seek medical care for a suspected staph infection.
When skin is punctured or broken for any reason, staph bacteria can enter the wound and cause an infection. But good hygiene can prevent many staph infections. Learn more.
When skin is punctured or broken for any reason, staph bacteria can enter the wound and cause an infection. But good hygiene can prevent many staph infections. Learn more.
The result of (we think) a staph infection. Doctor cut it open and drained a lot of blood and puss from it and then bandaged it up and put me on antib...
Coloured scanning electron micrograph (SEM) of Staphylococcus warneri, coccoid, Gram-positive prokaryote. Staphylococcus warneri is a common commensal bacterium found on the skin of humans and animals. It is a coagulase-negative Staphylococcus sp. that rarely causes disease but it may cause infection in immunocompromised patients. It occasionally has been associated with cases of septicaemia and bacteraemia. Staphylococcus warneri is sensitive to the antibiotic, vancomycin. Magnification: x6,000 when shortest axis printed at 25 millimetres. - Stock Image F017/4255
Define Staphylococcus capitis. Staphylococcus capitis synonyms, Staphylococcus capitis pronunciation, Staphylococcus capitis translation, English dictionary definition of Staphylococcus capitis. n. pl. staph·y·lo·coc·ci Any of various spherical gram-positive parasitic bacteria of the genus Staphylococcus that usually occur in grapelike clusters and...
Patients admitted into the Intensive Care Unit (ICU) have an intravenous (IV) catheter (small plastic tube) placed in their vein. Very occasionally (4 times out of 100) the insertion of an intravenous catheter may cause an infection in the blood. It has been shown that the removal of the catheter and the insertion of a new one at a new site helps to get rid of this infection. Sometimes, antibiotics are also given.. Vancomycin is the antibiotic given intravenously (into the vein) to treat these catheter-related infections. At Vancouver General Hospital, some physicians may not give any vancomycin at all whereas others may treat with intravenous (IV) vancomycin for one to fourteen days.. Since there are a lack of data to support the length of IV vancomycin therapy, the investigators would like to find out if two days of IV vancomycin are as good as seven days.. Therefore, the purpose of this study is to determine if two days of IV vancomycin are as good as seven days for the treatment of ...
The book covers updated topics on the genus Staphylococcus, including the latest discoveries. In each chapter, the author discusses the results obtained and published during her more than 20 years as a researcher in this area. The book is divided into eight chapters, with a presentation of the genus Staphylococcus, current classification, general characteristics of these bacteria and the clinical significance of coagulase-negative staphylococci (CoNS), which are often classified as mere blood culture contaminants, but are the etiological agents most commonly associated with neonatal infections and peritonitis in patients undergoing peritoneal dialysis. Coagulase-negative staphylococci can colonize the surface of catheters and produce an extracellular polysaccharide that leads to the formation of biofilms which potentiate their pathogenicity. The book also focuses on the identification of CoNS, virulence factors responsible for the symptoms and severity of infections caused by Staphylococcus spp. ...
Sharing More than Friendship - Nasal Colonization with Coagulase-Positive Staphylococci CPS and Co-Habitation Aspects of Dogs and Their Owners. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Staphylococcus warneri lipase 2: A gene encoding an extracellular lipase was identified in Staphylococcus warneri 863; amino acid sequence in first source
Results: The results showed 91 isolates (56.88%) were coagulase-positive, and 69 isolates (43.12%) were coagulase-negative Staphylococcus aureus (CNSA). Out of 91 (56.88%) coagulase-positive staphylococci, 32 isolates (35.16%) were resistant to cefoxitin, and 30 isolates (32.96%) were resistant to oxacillin, using disc diffusion method. PCR revealed the presence of the femA gene (510 bp band) in all coagulase-positive isolates (100%), and the mecA gene (513 bp band) was detected in 32 isolates (35.16%); out of 32 MRSA isolates, 13 isolates (40.62%) were positive for presence of the luk-pv gene (433 bp band). ...
We have shown that there are at least three distinct types of SCCmec in the chromosome of MRSA worldwide. SCCmec was defined as the DNA element on the MRSA chromosome demarcated by a pair of direct repeats and inverted repeats, havingccr genes required for its movement and carrying themecA gene (14, 17). As far as we could judge from the structure of the two elements newly identified in this study, they seem to constitute a family of SCCmec together with N315-type SCCmec.. The mecA gene is considered to have originated in some coagulase-negative staphylococcus species (36) and was then transferred into S. aureus to generate MRSA (1,13, 32). It is likely that SCCmec serves as the carrier of the mecA gene moving across staphylococcal species, since mecA genes in other staphylococcal species have never been found without the accompaniment of SCCmec-like structure (T. Ito and Y. Katayama, unpublished observation). Since both ccrA and ccrB genes are required for the integration event, we considered ...
Name: Staphylococcus warneri Kloos and Schleifer 1975 (Approved Lists 1980). Category: Species. Proposed as: sp. nov.. Etymology: N.L. gen. masc. n. warneri, of Warner, named for Arthur Warner, from whom this organism was originally isolated Type strains: ATCC 27836; CCUG 7325; CIP 81.65; DSM 20316; JCM 2415; LMG 13354; NCTC 11044; NRRL B-14736 See detailed strain information at ...
Staphylococci are the most abundant skin-colonizing bacteria and the most important causes of nosocomial infections and community-associated skin infections. and birds [1]. Two main groups are distinguished by their ability to coagulate blood: coagulase-positive staphylococci, with the most important species being or as a subject, owing to its eminent role in human contamination. The nose is the most important site PNU 282987 of colonization [3], but is situated in the pharynx also, perineum, axillae and on your skin (predominantly over the hands, upper body and abdomen) [4C6]. Consistent colonization with is normally observed in around 20% of the populace, while 30% bring transiently, and around 50% are non-carriers [7,8]. In consistent providers, who all possess within their noses, the regularity of colonization of various other body sites is normally increased weighed against the general people [9]. Consistent carriage prices are higher in kids than adults [4]. Oddly enough, theres been a ...
Seventy-three percent of coagulase negative Staphylococci (CoNS) Isolated from blood cultures were contaminants and 34% of patients with contaminating CoNS were treated with specific anti-staphylococcal antibiotics including vancomycin. This paper underlines the need for careful evaluation of CoNS from blood culture before instituting therapy ...
If you consider that MRSA causes meningitis, pneumonia and toxic shock syndrome and is scary, we have other nasty bacteria in our hospitals; E. Coli cases up by 48% in 2007; Clostridium difficile showing a 72% rise in 2007 with over 5,000 deaths attributed to it; Klebsiella pneumoniae 5,198 cases hitting and killing people who have underlying lung problems (causes abscesses in the lungs); Streptococcus pneumoniae 4,553 cases and 14,943 cases of Coagulase negative staphylococcus causing bloodstream infections. In the USA, C. difficile was responsible for 18,650 deaths last year and now exceeds AIDs deaths there. What are the real risks today in the UK concerning acquiring a serious hospital infection? High , is the probable answer.. The historical period of effective anti-biotics has perhaps now passed after 50 years and we need to re-institute simple Victorian inspired hygiene measures that lapsed because we had the anti-biotics to sort out problems caused by slack hygiene. Anyone who remembers ...
To introduce and demonstrate the principle and experimental set up for determining the microbes ability to detoxify hydrogen peroxide and /or to cause blood coagulation.
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Abstract. This study included collection of 100 specimens from patients in AL-Kindy Teaching Hospital and teaching laboratories of Medical City Hospitals in Baghdad during the period from August to December 2012 ,these specimens differed in their sources which included 19 nasal swab, 16 wound swab,27 burn swab, 7 pus, 15 sputum, 10 corneal swab and 6 urine . Only 38 (38%) isolates was identified as Staphylococcus. In this study, 29 isolates (76.3%) were coagulase-positive (COPS), while only 9 isolates(23.6%) were coagulase negative (CONS), from total 38 isolates of Staphylococci. The distribution of Methicillin resistance among Staphylococcus spp. was investigated by disc diffusion method. In this study, 21 isolates (55.26%) showed resistant to the Methicillin while 17 isolates (44.73%) were sensitive. The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA was produced slime layer when tested by tube method, but the amounts of ...
Buy ISO 6888-1:1999/Amd 1:2003 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 1: Technique using Baird-Parker agar medium - Amendment 1: Inclusion of precision data from SAI Global
Design: DNA was extracted using UltraClean microbial DNA isolation kit (Mo Bio). Shotgun sequencing libraries were prepared using Fragmentase (Epicentre BioTechnologies), standard Y-adaptor ligation, PCR amplification using Kapa HiFi (KAPA Biosystems), and were purified using Agencourt AMPure XP (Beckman Coulter). ...
This paper reports the results of a pilot study, which examines the respiratory control exerted by chest and abdominal-muscles… Expand ...
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Health care professionals can determine that its a staph infection by taking a culture (a swab with a giant Q-tip) from the infected site. Once staph is diagnosed, your provider prescribes antibiotics that kill that specific bacterial strain.. Hospitals are trying to stop staph infections, because most hospital patients are at risk, and because drug-resistant strains of staph (versions of the bacteria not vulnerable to the antibiotics used to treat staph infections) are becoming more common. People with resistant staph infections may require hospitalization to receive antibiotics through an IV or by injection.. As far as we know, however, bacteria do not develop resistance to the complex natural antibiotics in herbs. ...
How to Prevent Staph Infection. Staphylococcus aureus, or staph, is a very common type of bacteria. According to the Center for Disease Control and Prevention (the CDC), 30% of people have the staph bacteria in their noses, and 20% of...