TY - JOUR. T1 - Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations. AU - Lee, Gabsang. AU - Hyun, Sang Hwan. AU - Kim, Hye Soo. AU - Kim, Dae Young. AU - Lee, So Hyun. AU - Lim, Jeong Mook. AU - Lee, Eun Song. AU - Kang, Sung Keun. AU - Lee, Byeong Chun. AU - Hwang, Woo Suk. PY - 2003/1/1. Y1 - 2003/1/1. N2 - This study was conducted to improve a porcine somatic cell nuclear transfer (SCNT) technique by optimizing donor cell and recipient oocyte preparations. Adult and fetal fibroblasts, and cumulus and oviduct cells were used as donor cells, and in vivo- and in vitro-matured oocytes were employed as recipient oocytes. The percentages of fusion and development to the blastocyst stage, the ratio of blastocysts to 2-cell embryos, and cell number of blastocysts were monitored as experimental parameters. In Experiment 1, donor cells of four different types were transferred to enucleated oocytes matured in vitro, and more (P , ...

Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ??mercaptoethanol (??ME) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than ...

Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells - Canine;Interspecies;Porcine;Somatic cell Nuclear Transfer;

Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro‐matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM‐199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1 kV/cm for 30 μs in 0.3 M mannitol medium. The activated cloned embryos were cul‐ tured in porcine zygote medium‐3 (PZM‐3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos recon‐ structed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to

WEDNESDAY, Jan. 24, 2018 (HealthDay News) -- Cloning cynomolgus monkeys (Macaca fascicularis) is feasible by somatic cell nuclear transfer (SCNT) using fetal fibroblasts, according to a study published online Jan. 24 in Cell.. Zhen Liu, from the Chinese Academy of Sciences Institute of Neuroscience in Shanghai, and colleagues examined the feasibility of cloning cynomolgus monkeys by SCNT to generate genetically uniform non-human primates for establishing animal models for research.. The researchers found that following SCNT, injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage correlated with improvement in blastocyst development and the rate of pregnancy of transplanted SCNT embryos in surrogate monkeys. Six pregnancies were confirmed in 21 surrogates for SCNT using fetal monkey fibroblasts, yielding two healthy babies. Twenty-two pregnancies were confirmed in 42 surrogates for SCNT using adult monkey cumulus cells, ...

Somatic cell nuclear transfer (SCNT) (cloning), being a reproductive or therapeutic method, and mitochondrial DNA transfer, as a strategy to avoid the transmission of mitochondrial diseases, are analyzed within this paper from a bioethics perspective. reduction of individual embryos delimits the moral acceptability of the promising techniques. research CDC42BPA related to the creation of individual Taxifolin pontent inhibitor blastocysts by SCNT. IN-MAY 2005, another group led by Hwang released articles (also in (Kennedy 2006). Around once, Stojkovic et al. (2005) also released a study where they too mentioned that that they had cloned individual embryos towards the blastocyst stage, and they also were regarded as the first to accomplish this technological feat. However, they were unable to derive ESC lines from your biological entities produced by them, so this work could not be considered as an objective demonstration of human being SCNT either. In 2006, Zavos and Illmensee (Zavos and ...

This study investigated whether four cumulus-specific genes: follicular stimulating hormone receptor (FSHr), hyaluronan synthase 2 (Has2), prostaglandin synthase 2 (Ptgs2) and steroidogenic acute regulator protein (Star), were correctly reprogrammed to be transcriptionally silent following somatic cell nuclear transfer (SCNT) in a murine model. Cumulus cells of C57xCBA F1 female mouse were injected into enucleated oocytes, followed by activation in 10 micromol/L strontium chloride for 5 h and subsequent in vitro culture up to the blastocyst stage. Expression of cumulus-specific genes in SCNT-derived embryos at 2-cell, 4-cell and day 4.5 blastocyst stages was compared with corresponding in vivo fertilized embryos by real-time PCR. It was demonstrated that immediately after the first cell cycle, SCNT-derived 2-cell stage embryos did not express all four cumulus-specific genes, which continually remained silent at the 4-cell and blastocyst stages. It is therefore concluded that all four ...

Fingerprint Dive into the research topics of Epigenetic reprogramming by somatic cell nuclear transfer in primates. Together they form a unique fingerprint. ...

I know I wrote an article on this type of media bias just a few days ago. But, they just keep on coming. Perhaps, I should start collecting the erroneous/inaccurate descriptions of somatic cell nuclear transfer and therapeutic cloning published in ...

Nobel Prize-winning geneticist Joshua Lederberg advocated cloning and genetic engineering in an article in The American Naturalist in 1966 and again, the following year, in The Washington Post.[3] He sparked a debate with conservative bioethicist Leon Kass, who wrote at the time that the programmed reproduction of man will, in fact, dehumanize him. Another Nobel Laureate, James D. Watson, publicized the potential and the perils of cloning in his Atlantic Monthly essay, Moving Toward the Clonal Man, in 1971.[4] With the cloning of a sheep known as Dolly in 1996 by somatic cell nuclear transfer (SCNT), the idea of human cloning became a hot debate topic.[5] Many nations outlawed it, while a few scientists promised to make a clone within the next few years. The first hybrid human clone was created in November 1998, by Advanced Cell Technology. It was created using SCNT - a nucleus was taken from a mans leg cell and inserted into a cows egg from which the nucleus had been removed, and the ...

MEPs beefed up the European Commissions initial proposal, citing high mortality rates at all development stages of cloning and EU citizens animal welfare and ethical concerns.. Due to the negative effects on animal welfare, cloning for farming purposes is rejected by a large majority of consumers. Furthermore, we do not need cloning to ensure meat supplies in the EU. Prohibiting cloning is therefore a matter of European values and principles. Consequently, the ban should apply not only to clones themselves but also to their reproductive material (semen and embryos), their descendants and any products derived from them, including imports. This is necessary because otherwise we would merely promote cloning in third countries, said Environment Committee co-rapporteur Renate Sommer (EPP, DE).. There are two key points that we focused on from the outset: protecting the health of EU citizens and consumers and extending the ban to cover the descendants of cloned animals, said co-rapporteur law ...

Researchers from the CAS Center for Excellence in Brain Science and Intelligence Technology, the Institute of Neuroscience of CAS and the Shanghai Research Center for Brain Science and Brain-inspired Intelligence cloned five macaque monkeys from the skin fibroblasts of a gene-edited BMAL1-deficient monkey using somatic cell nucleus transfer (SCNT). The BMAL1-deficient monkeys exhibited circadian disruption, sleep deprivation, anxiety, depression and schizophrenia-like sensory processing impairment. Transcriptome analysis also revealed elevated inflammation and stress response in the BMAL1-deficient monkeys. Their condition suggested they could be used to model human maladies such as sleep deprivation, major depressive disorder, and perhaps aging. This represents the first time anywhere in the world that gene-edited macaque monkeys of uniform genetic background have been successfully produced. This advance will help propel research on the mechanisms of human brain disease and early diagnosis and ...

The 2012 Nobel Prize in Physiology or Medicine was awarded jointly to Sir John B. Gurdon and Shinya Yamanaka for the discovery that mature cells can be reprogrammed to become pluripotent. Sir John Gordon pioneered the field of somatic cell nuclear transfer, wherein the nucleus of a mature cell is transplanted into an enucleated egg, to produce a living organism (tadpole).1 The technique, which is commonly referred to as cloning, produced a paradigm shift in developmental biology and paved the way for genome reprogramming for reproductive gains. It led to subsequent cloning of a dozen or so species, with Dolly the sheep being the most famous cloned animal, cloned by Ian Wilmut and colleagues at the Roslin Institute in Scotland in July 1996.2 Although Dolly was euthanized in 2003 because of progressive lung and degenerative joint diseases, the success of the nuclear transfer technique demonstrated that the genome, even when isolated from adult cells, contains the information necessary to ...

In summary, human cloning is a prominent social issue in the world. Even though it is not yet scientifically possible, there is a great deal to be said about it in the way of ethics. Though polls indicate that the general public is against the idea of cloning, there are a variety of opinions on the topic that each has support. It is thought by some that the legalization of cloning could greatly benefit the world as it could be the best way to produce the next generation of human beings. However, many see ethical dilemmas in this outlook and think that cloning should be forbidden entirely. It could spark the dehumanization of our race by turning human lives into something of a commodity and it could also cause a lot of social distress. Finally, the third proposition is that there should be a middle ground. There is a lot of benefit in therapeutic cloning without anywhere near as much of the ethical cost. By legalizing this form of cloning only, research could continue, albeit with limitations. ...

On May 8, 2014, the Federal Circuit released its latest precedential opinion starring Dolly the Sheep. The tale began in 1996 when Dolly the Sheep became the first mammal ever cloned from an adult somatic cell through the successful work of Keith Henry Stockman Campbell and Ian Wilmut. Campbell and Wilmut patented their breakthrough method of cloning mammals using somatic cells. In addition, they applied for a patent claiming the actual cloned animals themselves-U.S. Patent Application No. 09/225,233 (the 233 application).. Keep Reading at: http://www.forbes.com/sites/danielfisher/2014/06/04/dolly-the-sheep-loses-patent-protection-but-could-other-clones-get-it/. ...

Somatic cell nuclear transfer (SCNT) has emerged as an important tool for producing transgenic animals and deriving transgenic embryonic stem cells. The process of SCNT involves fusion of in vitro matured oocytes with somatic cells to make embryos that are transgenic when the nuclear donor somatic cells carry foreign DNA and are clones when all the donor cells are genetically identical. However, in canines, it is difficult to obtain enough mature oocytes for successful SCNT due to the very low efficiency of in vitro oocyte maturation in this species that hinders canine transgenic cloning. One solution is to use oocytes from a different species or even a different genus, such as bovine oocytes, that can be matured easily in vitro. Accordingly, the aim of this study was: (1) to establish a canine fetal fibroblast line transfected with the green fluorescent protein (GFP) gene; and (2) to investigate in vitro embryonic development of canine cloned embryos derived from transgenic and non-transgenic ...

Biotechnology allows us to use living organisms or their processes for human needs or purposes. Currently, this topic includes such general examples as cloning, stem cells (adult, umbilical cord, and embryonic), DNA.

Nuclear transfer (NT) is a technique used to investigate the development and reprogramming potential of a single cell. DNA methyltransferase-3-like, which has been characterized as a repressive transcriptional regulator, is expressed in naturally fertilized egg and morula/blastocyst at pre-implantation stages. In this study, we demonstrate that the use of Dnmt3l-knockout (Dnmt3l-KO) donor cells in combination with Trichostatin A treatment improved the developmental efficiency and quality of the cloned embryos. Compared with the WT group, Dnmt3l-KO donor cell-derived cloned embryos exhibited increased cell numbers as well as restricted OCT4 expression in the inner cell mass (ICM) and silencing of transposable elements at the blastocyst stage. In addition, our results indicate that zygotic Dnmt3l is dispensable for cloned embryo development at pre-implantation stages. In Dnmt3l-KO mouse embryonic fibroblasts, we observed reduced nuclear localization of HDAC1, increased levels of the active histone ...

An international team, headed by researchers at the University of California, San Diego School of Medicine, has identified a key enzyme in the reprogramming process that promotes malignant stem cell cloning and the growth of chronic myeloid leukemia (CML), a cancer of the blood and marrow that experts say is increasing in prevalence. ... Read the…

An international team, headed by researchers at the University of California, San Diego School of Medicine, has identified a key enzyme in the reprogramming process that promotes malignant stem cell cloning and the growth of chronic myeloid leukemia (CML), a cancer of the blood and marrow that experts say is increasing in prevalence.

A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patients particular disease. By doing so, an in vitro model could be created, would be useful for studying that particular disease, potentially discovering its pathophysiology, and discovering therapies.[5] For example, if a person with Parkinsons disease donated his or her somatic cells, the stem cells resulting from SCNT would have genes that contribute to Parkinsons disease. The disease specific stem cell lines could then be studied in order to better understand the condition.[6]. Another application of SCNT stem cell research is using the patient specific stem cell lines to generate tissues or even organs for transplant into the specific patient.[7] The resulting cells would be genetically identical to the somatic cell donor, thus avoiding any complications from immune system rejection.[6][8]. Only a handful of the labs in the world are currently using SCNT techniques ...

Single cell cloning and recombinant monoclonal antibodies generation from RA synovial B cells reveal frequent targeting of citrullinated histones of NETs ...

Thats because meat from clones and their offspring isnt labeled. In 2008, the U.S. Food and Drug Administration decided that food from cloned animals doesnt need to be. In the years since, the Biotechnology Industry Organization-a trade group that represents ViaGen and other biotech firms-and the meat industry successfully defeated several bills in Congress that would have required labels for cloned food. The FDA argues that it would be too difficult to label such products anyway. Theres no difference between [cloned products] and food produced by conventional methods. Theres really nothing for us to label, said Dr. Stephen Sundlof, then director of the FDAs Center for Food Safety and Applied Nutrition, during a 2008 press conference.. The FDA and the biotech industry claim that food from clones is safe, but some scientists say there isnt enough evidence to know for certain. To date, no large-scale studies have been done on the health effects of eating meat from cloned animals. The most ...

Stem-cell research lab in the Czech Republic (file photo) February 1997 -- Scotlands Roslin Institute announces it has cloned a sheep from cells taken from an adult ewe. The sheep, Dolly, was the ...

Ian Wilmut is a embryologist and genetic engineer who was the leader of the first research group to successfully clone an animal. He is also credited for birthing the first animal from a frozen embryo. Currently, he serves as the Chair for the Scottish Center for Regenerative Medicine at the University of Edinburgh. The creation of the first cloned animal had created a controversy in the world of engineering and science. Ethical questions have been raised with regard to cloning technology and have shaped the ways in which the global community views genetic engineering[1]. Ian Wilmut was born on July 7th, 1944 in Hampton Lucey, England, and raised in the town of Coventry. [2]. While attending the Scarborough High School for boys, he met with Gordan Whalley, head of the biology department. Despite considering himself an average student at best, Ian enjoyed the scientific research given to him by Whalley and the department, and slowly but surly took a steady interest in research.His true passion ...

The IOMS convened a seminar in 1983 on Reproduction in Islam, in which two papers were presented dealing with the possibility of human cloning as a Tesult of successful cloning in plants, frogs and small marine animals. The Semi1 nar made the following recommendation:. To exercise prudence in giving a Shariah- based opinion on human cloning (as achieved in animals) and to call for further medical and Islamic investigation of these issues. It would be possible to apply genetic engineering of micro-organisms using the recombinant DNA technology to produce medicinal substances in abundant supply .. Since 1993, when an identical twin was produced by splitting a fertilised egg, and later when cloning of Dolly the sheep was announced in February 1997, cloning has returned into the forefront of medical debate with much intensity and urgency. Then followed an announcement on the successful cloning of two monkeys at the University of Oregon in the United States of America. Since the techniques ...

An affiliate of LifeNews.com, this is 2 minutes of daily radio news to help form good pro-life thought and good pro-life action, by Jim ...

Dollys remains on display at the Royal Museum in Scotland. 1997: The world learns of the existence of Dolly the sheep, the worlds first successfully cloned mammal. Dolly (named for Dolly Parton, the buxom country-western singer, because it was a mammary cell used in the cloning process) was cloned at the Roslin Institute in Scotland. […]

2010 Cell Methods: [[Group 1 Project - Fluorescent-PCR,Group 1 - Fluorescent-PCR]] , [[Group 2 Project - RNA Interference,Group 2 - RNA Interference]] , [[Group 3 Project- Immunohistochemistry,Group 3 - Immunohistochemistry]] , [[Group 4 Project - Cell Culture,Group 4 - Cell Culture]] , [[Group 5 Project - Electron Microsopy,Group 5 - Electron Microsopy]] , [[Group 6 Project - Confocal Microscopy,Group 6 - Confocal Microscopy]] , [[Group 7 Project - Monoclonal Antibodies,Group 7 - Monoclonal Antibodies]] , [[Group 8 Project - Microarray,Group 8 - Microarray]] , [[Group 9 Project - Fluorescent Proteins,Group 9 - Fluorescent Proteins]] , [[Group 10 Project - Somatic Cell Nuclear Transfer,Group 10 - Somatic Cell Nuclear Transfer ...

2010 Cell Methods: [[Group 1 Project - Fluorescent-PCR,Group 1 - Fluorescent-PCR]] , [[Group 2 Project - RNA Interference,Group 2 - RNA Interference]] , [[Group 3 Project- Immunohistochemistry,Group 3 - Immunohistochemistry]] , [[Group 4 Project - Cell Culture,Group 4 - Cell Culture]] , [[Group 5 Project - Electron Microsopy,Group 5 - Electron Microsopy]] , [[Group 6 Project - Confocal Microscopy,Group 6 - Confocal Microscopy]] , [[Group 7 Project - Monoclonal Antibodies,Group 7 - Monoclonal Antibodies]] , [[Group 8 Project - Microarray,Group 8 - Microarray]] , [[Group 9 Project - Fluorescent Proteins,Group 9 - Fluorescent Proteins]] , [[Group 10 Project - Somatic Cell Nuclear Transfer,Group 10 - Somatic Cell Nuclear Transfer ...

Biology of Reproduction contains original scientific research on a broad range of topics in the field of reproductive biology, as well as minireviews.

Dolly was influential in Hoboken and in local Democratic Party circles. She used her knowledge of Italian dialects and fluent English to translate for immigrants during court proceedings, particularly those pertaining to requests for citizenship. This earned her the respect of local politicians, who made her a Democratic ward leader. She was the first immigrant woman to hold that position in her local third ward, where she reliably delivered as many as six hundred votes for Democratic candidates. In 1919, she chained herself to city hall in support of the Womens suffrage movement. She also worked as a midwife, earning $50 for each delivery, a fair amount of money at the time. These activities kept Dolly away from home during much of her sons childhood. According to Kaplan, Dolly also ran an illegal abortions service that earned the nickname Hatpin Dolly. Her reputation as an abortionist led one area church to ban her son from singing there ...

ZCZC MIATCDAT4 ALL TTAA00 KNHC DDHHMM TROPICAL DEPRESSION DOLLY DISCUSSION NUMBER 18 NWS TPC/NATIONAL HURRICANE CENTER MIAMI FL AL042008 500 PM EDT THU JUL 24 2008 DOLLY HAS CONTINUED TO WEAKEN AND IS NOW A TROPICAL DEPRESSION ACCOMPANIED BY A LARGE AREA OF RAIN AND SQUALLS. THE DEPRESSION IS WELL INLAND MOVING TOWARD THE WEST-NORTHWEST AT 12 KNOTS. THIS GENERAL MOTION IS FORECAST TO CONTINUE UNTIL DISSIPATION IN A DAY OR SO. THIS WILL BE LAST ADVISORY ISSUED BY THE NATIONAL HURRICANE CENTER ON DOLLY. FUTURE INFORMATION ON DOLLY CAN BE FOUND IN PUBLIC ADVISORIES ISSUED BY THE HYDROMETEOROLOGICAL PREDICTION CENTER... UNDER AWIPS HEADER TCPAT4 AND WMO HEADER WTNT34 KWNH...BEGINNING AT 10 PM CDT. FORECAST POSITIONS AND MAX WINDS INITIAL 24/2100Z 28.2N 100.6W 30 KT...INLAND 12HR VT 25/0600Z 29.0N 102.5W 25 KT...INLAND 24HR VT 25/1800Z 29.5N 104.5W 20 KT...REMNANT LOW 36HR VT 26/0600Z...DISSIPATED $$ FORECASTER AVILA NNNN ...

Escucha canciones y álbumes de Dolly, incluyendo Family (feat. Dora and Dolly), Roberta Flack (feat. Dolly), One, Two, Three (feat. Molly, Dolly & Tornillin) y mucho más. Gratis con la suscripción de Apple Music.

I know the Obama camp is fond of claiming that McCain-Palin would be more of the same, but that isnt quite accurate. America would find itself in a much worse position should McCain-Palin win in November. As reported over at Wired today, John McCain would make the simple act of researching somatic cell nuclear transfer (aka therapeutic cloning) illegal, going well-beyond the current Bush administration policy of simply not funding such research. I spent far too much of my life researching stem cell research as a research assitant in graduate school, so it is a topic that is (thankfully no longer) near and dear to my heart (Edit: I need to find a way to use the word research a fourth time in this sentence). The current Bush policy makes us look like morons to the rest of the world, who have been glibly blowing past the United States in this essential technical front. Bush himself is not opposed to stem cell research (or abortion, the issue at the heart of this debate), but uses the issue as ...

In our final issue this semester we open up our pages to the debate over stem cell cloning and DNA testing. On February 28, the U. S. House of Representatives passed a ban on all human cloning and blocked funding for cloned stem cell research. Awaiting passage in the Senate, the bill will put a stop to all stem cell research, as well as, any future human cloning. Supporters of the bill argue that it is immoral to destroy a cloned embryo for research because it has potential as a human being even before implantation in the womb. Congress must act now, said Rep. Sue Myrick, R-NC. We can no longer wait for another biotech company to claim they have cloned children. Anything other than a total ban would license the most ghoulish and dangerous enterprise in human history, she added. We cannot afford to treat the issue of human embryo cloning lightly, said Rep. Bart Stupak, D-Mich., co-author with Rep. Dave Weldon, R-Fla. of a complete ban bill. The human race is not open to experimentation ...

|Who knew there was a term for an animal/human hybrid? Actually, cybrid is short for cytoplasmic hybrid. Wesley Smith has already reported on the story that scientists in the UK - where such things are regulated by the government, unlike in the USA - have requested permission to produce a somatic cell nuclear transfer embryo…

I am a strong supporter of this technology (therapeutic cloning), in particular somatic cell nuclear transfer... Scientific endeavour has been an enduring area of interest to me. I have been particularly concerned with finding an appropriate balance between allowing the cutting-edge research and technology that we have to prosper and needing to protect our community through effective regulation of scientific activity...You need to understand how science progresses. It doesnt progress with a single step that means that you suddenly have cures. It moves incrementally towards a goal, and you gradually put in place bits of the jigsaw and solve various technical problems that are required... Those people who think that there is no moral problem with embryo research should be allowed to carry out that research and should not be prevented from doing so by the power of the law. Those people who think the research is wrong should be allowed to say so and to protest against what they believe to be wrong. ...

The present study was designed to evaluate the feasibility of producing pig transgenic blastocysts expressing enhanced green fluorescent protein (GFP) and to examine the effects of shape and preparation methods of donor cells on in vitro developmental ability of pig nuclear transferred embryos (NTEs). In experiment 1, the effect of GFP transfection on development of pig NTEs was evaluated. The cleavage and blastocyst rates showed no significant difference between NTEs derived from transfected and non-transfected donors. In experiment 2, the effect of different nuclear donor preparation methods on in vitro development of NTEs was examined. The cleavage rate showed no statistically significant differences among three preparation methods. The blastocyst rates of donor cells treated once at −4 °C and those of freshly digested cells were similar to each other (26.3% vs 17.9%). The lowest blastocyst rates (5.88%) were observed when cells cryopreserved at −196 °C were used as donors. In ...

Developmental Ability of Bovine Embryos Nuclear Transferred with Frozen-thawed or Cooled Donor Cells - Somatic Cell Nuclear Transfer;Frozen-thawed;Cooled;Apoptosis;Bovine;

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Study suggests additional hurdles to therapeutic cloning may lie ahead San Diego, Calif. -- Scientists from the Burnham Institute for Medical Research (BIMR) and Illumina Inc., in collaboration with stem cell researchers around the world, have found that the DNA of human embryonic stem cells is chemically modified in a characteristic, predictable pattern. This pattern distinguishes human embryonic stem cells from normal adult cells and cell lines, including cancer cells. The study, which appears online today in Genome Research, should help researchers understand how epigenetic factors contribute to self-renewal and developmental pluripotence, unique characteristics of human embryonic stem cells that may one day allow them to be used to replace diseased or damaged cells with healthy ones in a process called therapeutic cloning. Embryonic stem cells are derived from embryos that are undergoing a period of intense cellular activity, including the chemical addition of methyl groups to specific DNA ...

At this site, Catholics can find information to better understand stem cell research, therapeutic and reproductive cloning, genetic testing, and much more. The Topics section has articles covering various technologies; what is moral, what is immoral. It also has articles on pertinent topics by other authors. The Books section has a reading list for those who want to do their own research. The Links page has a list of websites through which one can keep up to date in this rapidly changing field. The Glossary page lists important terms and their definitions. The Church Teaching page has official Catholic Church teaching on reproductive issues and the sanctity of human life. The Blog has my daily thoughts on new developments and a chance for you to respond. And my favorite, the Quotes section, has all the verbal gems I have found that say it all. ...

At this site, Catholics can find information to better understand stem cell research, therapeutic and reproductive cloning, genetic testing, and much more. The Topics section has articles covering various technologies; what is moral, what is immoral. It also has articles on pertinent topics by other authors. The Books section has a reading list for those who want to do their own research. The Links page has a list of websites through which one can keep up to date in this rapidly changing field. The Glossary page lists important terms and their definitions. The Church Teaching page has official Catholic Church teaching on reproductive issues and the sanctity of human life. The Blog has my daily thoughts on new developments and a chance for you to respond. And my favorite, the Quotes section, has all the verbal gems I have found that say it all. ...

The developmental efficiency of somatic cell nuclear transfer has slowly improved over the past five years. Dr. Wilmut achieved a developmental efficiency (recipient oocytes to offspring obtained) of 0.4% in 1996 (Wilmut et al 1997). Dr. Wakayama managed to improve this to 2.8% in subsequent murine research, by using microinjection rather than electrofusion (and other factors) (Wakayama et al 1998). However, the efficiency of development from adult mammalian somatic cells has remained at around 2% since that time (Polejaeva et al 2000). It is anticipated that for every one hundred nuclear transplant embryos, only one or two embryos will result in healthy developmentally normal offspring (Colman 2000). It should be emphasized that this does not mean that 98% of the live-born offspring will be developmentally abnormal, the vast majority of nuclear transplant zygotes do not even get implanted into the uterus. From the scientific literature over the past few years, an averaged developmental ...

What I hope to learn from this topic is what is therapeutic cloning and how it is used today. In addition, I would also like to learn, in general, more about therapeutic cloning. I would like to know what this type of cloning has done from when it was discovered till now and if they are making more discoveries on this topic. Anything to do with stem cells, I am for it. I came to this decision from many different things. This came from personal experiences, because I knew people personal who had treatments done by some type of stem cell treatment. Those treatments helped. Also my teachers daughter was diagnosed with cancer and thanks to stem cells, it had helped her make a recovery. This position also reflects what I have heard from my family. My family is for stem cell treatments of any kind. What me and my family believe is that if it helps you with a disease then it is a good thing. Yeah it may have some negative sides but any treatment or anything in general with have people looking on the ...

Interesting post Erick. I think Adleman read the bill. I guess the whole controversy depends on the interpretation of the definition of human cloning. Both bills prohibit it and both bills provide for the same criminal punishment for engaging in cloning.. However, Adlemans bill defines cloning as the asexual genetic replication of a human being by transferring a preimplantation embryo that has been created by somatic cell nuclear transfer, parthenogenesis, or by other asexual means into a uterus or uterine-like environment with the purpose of creating a human fetus or a human child. Shafers bill defines cloning as human asexual reproduction accomplished by introducing nuclear material from one or more human somatic cells into a fertilized or unfertilized oocyte whose nuclear material has been removed or inactivated so as to produce a living organism at any stage of development that is genetically virtually identical to an existing or previously existing human organism.. Now, Im not a ...

TY - JOUR. T1 - Rhesus monkey embryos produced by nuclear transfer from embryonic blastomeres or somatic cells. AU - Mitalipov, Shoukhrat M.. AU - Yeoman, Richard R.. AU - Nusser, Kevin D.. AU - Wolf, Don P.. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2002. Y1 - 2002. N2 - Production of genetically identical nonhuman primates would reduce the number of animals required for biomedical research and dramatically impact studies pertaining to immune system function, such as development of the human-immuno-deficiency-virus vaccine. Our long-term goal is to develop robust somatic cell cloning and/or twinning protocols in the rhesus macaque. The objective of this study was to determine the developmental competence of nuclear transfer (NT) embryos derived from embryonic blastomeres (embryonic cell NT) or fetal fibroblasts (somatic cell NT) as a first step in the production of rhesus monkeys by somatic cell cloning. Development of cleaved embryos up to the 8-cell stage was ...

E advantages and disadvantages of cloning. Has helped over eleven million people protect themselves from scams? Scambusters is committed to helping you avoid getting. Me people think that cloning is great. Oning technology has received great attention in recent. Has helped over eleven million people protect themselves from scams. E to my naturally. Scambusters is committed to helping you avoid getting. Sed on human ethic cloning takes away the ethical issues that are crucial to human. Uman Cloning Human cloning is the process of transplanting nuclear DNA from an adult. R argumentative essays. Essay Presentation on Human Cloning. human stem cell research and experimentation: all sides to the debateEssay on cloning and genetic engineering. essay on ishant sharma height Human cloning Summary. Since November 1994, Scambusters? Ture of Human Cloning in America! En against cloning may. a short essay on food security in india, a2 english lit lang coursework, advantages of human cloning essay a short ...

The objectives of the present study were to initiate cloning of Korean native goat by somatic cell nuclear transfer (NT) and to examine whether unovulated (follicular) oocytes can support the same developmental ability of NT embryos as ovulated (oviductal) oocytes after hCG injection in stimulated cycles of the goat. The in vivo-matured and immature oocytes were collected from the oviducts and follicles of superovulated does, respectively, and the immature oocytes were maturated in vitro. Ear skin fibroblasts derived from a 3-yr-old female Korean native goat were used as the donors of nuclei or karyoplasts. Following fusion, activation and in vitro culture to a 2- to 4-cell stage, 49 in vitro-derived and 105 in vivo-derived embryos were transferred to 6 and 17 recipient does, respectively. One doe and three does of the respective groups were identified as pregnant by ultrasonography on day 30 after embryo transfer. However, only one doe, which had received in vivo-derived embryos, delivered a ...

California in the U.S. supports embryonic stem cell research through Proposition 71, a $3 billion bonding initiative that is projected to provide about $300 million in stem cell research funding annually for 10 years. Approved by California voters Nov. 2, 2004, Proposition 71 establishes a state constitutional right to pursue stem cell research, including through SCNT or research/therapeutic cloning, and prohibits funding of human reproductive cloning research ...

Metoda jedrnega prenosa t.i. somatic cell nuclear transfer (SCNT) je pogosto uporabljena tehnika za kloniranje prašičev. Slabost te metode je njena nizka učinkovitost (ok. 1-3%). Na učinkovitost vpliva tudi sposobnost reprogramiranja donorske celice do pluripotentne. Kot donorske celice večinoma uporabljajo končno diferencirane fibroblasti, ki se pogosto nepravilno reprogramirajo, kar je eden od vzrokov za tako nizko učinkovitost metode. Oocite lažje reprogramirajo genom nediferenciranih ali manj diferenciranih celic kot genom končno diferenciranih celic. Ker embrionalne matične celice (ESC) prašičev niso dostopne, se uporabljajo multipotentne mezenhimske matične celice (MSC). Avtorji članka Bone Marrow Mesenchymal Stem Cells Are an Attractive Donor Cell Type for Production of Cloned Pigs As Well As Genetically Modified Cloned Pigs by Somatic Cell Nuclear Tranfer Li in sod. so v raziskavi primerjali sposobnost razvoja kloniranih prašičjih embrijev, nastalih iz MSC ali ...

Dolly magazine has been somewhat of an institution in the Australian media landscape, and for the young girls growing up, it was a bible - along with Girlfriend - to all things adolescence. But more importantly, it was Dolly Doctor - the sealed section in the middle - that was most revered.. Dolly kicked off in 1970, and on Wednesday, publisher Bauer Media announced would be finishing its print editions this December, just shy of its 47th birthday.. The mag had suffered severe drops in recent circulation reports, dropping 26 per cent to 30,010 in year-on-year sales for 2016.. And while the digital edition of the Dolly brand will keep its content alive - Dolly Doctor included - we thought it best to honour the sacred ripping of the sealed section with a recap of nine of the most amusing and confusing health questions sent to Dolly Doctor over the years.. After all, a recent study showed that the advice that sprung from this health Q&A are more factual than any other magazine in the country. Cant ...

In no case shall state facilities, state funds, fees, or charges, or investment income on state funds be used to create a human embryo by somatic cell nuclear transfer for any purpose. For purposes of the Act, human embryo means the developing human organism from the time of fertilization until the end of the eighth week of gestation and includes an embryo or developing human organism created by somatic cell nuclear transfer; somatic cell nuclear transfer means a technique in which the nucleus of an oocyte is replaced with the nucleus of a somatic cell. ...

Nikolas T. Nikas, president and general counsel of Bioethics Defense Fund (BDF), will be coming to speak to the Vanderbilt community about the law, science and ethics surrounding human cloning, animal-human hybrids, and 3-parent embryos. Nikas is known for his expertise in clearly integrating the principles of natural law and political theory with the facts of science and medicine to be used as the foundation for strategic legislation, litigation, education and media. Nikas is consulted across the nation and the world on issues of abortion, healthcare rights of conscience, human cloning/embryonic stem cell research, and physician assisted suicide. He is a dynamic speaker, and gave a well-attended lecture on the scientific/medical details, as well as the ethical issues, with stem cell research in 2013. The Society of Saints Cosmas and Damian is delighted to welcome him back to campus.. FREE lunch!! will be provided for the first 75 in attendance, donated by the SSCD faculty adviser, Dr. Wes ...

I think that an overwhelming majority of American citizens today would say that you shouldnt create human beings to experiment on them or for spare parts, Bond said. The journal supports a plan by Democratic Sens. Edward Kennedy of Massachusetts and Dianne Feinstein of California that would place a 10-year ban on human cloning but allow the cloning of human cells and tissues for research. Current law bans federally funded research on human embroyos, but not private work. The Feinstein-Kennedy proposal would allow such research up to the point of transferring cloned cells to a womans uterus. The Republican and Democratic plans have stalled in the Senate because of a lack of common ground between the two proposed bills. The journal also urged scientists to engage in an honest debate with the general public on the use of human cloning. We need to think about what, if any, circumstances might warrant cloning, as well as the circumstances under which it should never be allowed, the editorial ...

Text of S. 1036 (110th): Human Cloning Prohibition Act of ... as of Mar 29, 2007 (Introduced version). S. 1036 (110th): Human Cloning Prohibition Act of 2007

Somatic cell nuclear transfer (SCNT), or cloning, is a form of artificial reproductive technology that can be used to improve economic traits of domestic animals. However, extreme inefficiency of producing viable offspring via this method is a major limitation. An aggressive immune response at the maternal-fetal interface is an important reason for SCNT pregnancy loss. The goal of this project was to investigate the molecular mechanisms of immune-mediated miscarriage in cloned cattle pregnancies. Many publications hint that immune-mediated miscarriage is associated with abnormal MHC-I expression in the placenta. The regulation of bovine MHC-I genes was systematically studied to identify the cause of abnormal MHC-I expression during immune-mediated miscarriage. We also produced cloned pregnancies to study immune- mediated pregnancy loss. MHC-I and cytokines involved in proinflammatory responses were highly expressed in the placental trophoblast cells of cloned fetuses and in the uterine endometrium of

Cloning technology has hit the headlines again recently, with the successful cloning of Nicky, a domestic cat as a replacement for a much-loved pet. In the coming year, we may also be hearing reports of the cloning of less well-known cat species.. One of the applications of cloning technology is the preservation of endangered species. Conservationists are currently looking at cloning as a promising technique in maintaining numbers of the South African black-footed cat, a small and elusive species. Following this, we may be seeing clones of the worlds smallest cat, the rusty spotted cat of India and Sri Lanka.. The first successful cat clone, cc, was born in December 2001, following work done by Martha Gomez and her team at the Audubon Center for Research of Endangered Species in New Orleans. This opened the door to the use of cloning as a conservation methodology. The first experiments involved the African wild cat, Felis lybica, due to its similarity with the domestic cat. In 2003, African ...

The first successful cloning experiments in vertebrates arose from the desire of embryologists to know whether the process of cell differentiation from an egg involved permanent or stable changes in the genome. One idea was that, as cells differentiate, the genes no longer needed (such as skin genes in intestinal cells) could be lost or permanently repressed. The other idea was that all genes are present in all cell types, and that cell differentiation involved the selective activation and repression of genes appropriate to the cell type. The transfer of nuclei from differentiated cells to an egg could answer these important questions. In 1952, the first successful transplantation of nuclei from early embryo cells was achieved with the American frog Rana pipiens.[8]. In the late 1980s, scientists took cloning to the next stage by cloning other mammals (cattle, sheep, pigs, mice, and rhesus monkeys).[9] This is seen as the first step toward the cloning of mammals closest to humans. But they were ...

GRTL strongly advocates for the passage of tightly written legislation at the national and state level that will permanently ban all human cloning including research on embryos. If human cloning proceeds, our minds can conjure up many scenarios of abuse of human cloning as our society creates human beings not in Gods own image but in our own.. Human cloning is an inherent violation of human dignity. As with abortion and assisted reproductive technologies, such as in vitro fertilization, human cloning denies the most fundamental of human rights - the right to life. The research process inevitably requires scientists to destroy and discard their failed experiments. For example, it took 277 attempts at cell manipulation and 29 embryo implants before the sheep, Dolly, was produced.. Cloning would further violate human dignity by denying the intrinsic value and uniqueness of each human life, thereby viewing human beings as products or commodities. For this same reason we already oppose surrogate ...

In somatic cell nuclear transfer in mammals, to clone a piglet is still a big challenge. Although many factors could contribute to the low success rate, such as quality of donor and recipient cells, types of donor cell including sources of animal breeds and tissues, number of passages and culture conditions, timing of cell cycle, procedures of nuclear transfer, techniques and embryos transfer, one of the factors is believed to be poor oocyte activation, especially in pig nuclear transfer. Therefore studies presented in this thesis aimed at the establishment of an in vitro culture system for pig oocyte maturation and embryo culture, based on this system an electrical activation protocol for pig oocytes was optimized and also tested by monitoring in vivo development of activated pig oocytes. Finally, the protocol was used for activating pig embryos reconstructed by transfer of somatic cells into enucleated ovulated oocytes and for production of pig parthenotes to maintain pregnancies of cloned pig ...

Paste as Plain TextPaste from WordRemove formattingInsert custom characterOutdentIndentUndo (Ctrl + Z)Redo (Ctrl + Y)Help (Alt + Shift + H). Apart from being praised as an ethical work-around to the problems of destroying early-stage human embryos-often about to be discarded as surplusage by fertility clinics - to obtain human pluripotent stem cells, Revazova et al. (U.S. Pat. No. 7732202 - copy at end of post) got relatively little attention from the IP community. I predict that the relatively benign reception that this patent got will change as various groups begin to sort through the claims and 138 columns of disclosure. Dolly, the cloned ewe, was made by removing the nucleus from an oocyte, or unfertilized ovum, of one breed of sheep, and replacing it with nuclear material from an adult donor cell taken from a different breed of sheep. The renucleated oocyte was induced to divide by an electrical pulse, and reimplanted in a surrogate mother, that eventually gave birth to Dolly, who looked ...

Interspecies somatic cell nuclear transfer (iSCNT) has been regarded as a potential alternative for rescuing highly endangered species and can be used as a model for studying nuclear-cytoplasmic interactions. However, iSCNT embryos often fail to produce viable offspring. The alterations in normal molecular mechanisms contributing to extremely poor development are for the most part unknown. Przewalskis gazelle-bovine iSCNT embryos (PBNT) were produced by transferring Przewalskis gazelle fibroblast nuclei into enucleated bovine oocytes. The percentages of PBNT embryos that developed to morula/blastocyst stages were extremely low even with the use of various treatments that included different SCNT protocols and treatment of embryos with small molecules. Transcriptional microarray analyses of the cloned embryos showed that the upregulation of reprogramming-associated genes in bovine-bovine SCNT (BBNT) embryos was significantly higher than those observed in PBNT embryos (1527:643). In all, 139 transcripts

In late 2003, two international bodies were unable to resolve disagreements that involved bioethical issues. First, the United Nations General Assembly failed to pass a treaty on reproductive cloning because of insistence by some countries that the treaty include a ban on cloning for research. In view of the importance of enacting prohibition of reproductive cloning, the two issues should be separated and each argued on its own merits. Relevant objections to separation of the two issues can be refuted. Second, the European Union (EU) failed to agree on conditions for funding stem-cell research because of the diversity of views and policies of the countries of the EU. Because a stalemate was reached, funding decisions in the next programme cycle will be made on an ad hoc basis. Scientists will not have information they need to plan research programmes, suggesting that clear guidelines, even if restrictive, are preferable to vague unpublicised criteria. ...

175, This story has been shared 113 times. The Stand Episode 5 Recap: What Happens in Vegas... The Stand Episode 4: Mother, Do You Think Theyll Drop the Bomb? 2:44 ... Dolly Parton Silver Threads and Golden Needles Dolly Parton, Loretta Lynn & Tammy Wynette More by Dolly Parton. The stress became so severe that Hiltner ran away in the middle of the night. The track interpolates Kenny Rogers and Dolly Partons 1983 single Islands in the Stream and was produced by Wyclef Jean and Jerry Wonda Duplessis for Pras debut solo studio album, Ghetto Supastar. Country icon and national treasure Dolly Parton has revealed the reason she declined a chance to sing with Pop superstar Katy Perry during the 2015 Super Bowl in Arizona. Bridgerton Season 2: Why Were Dying to See Kate Sheffield, Francesca Bridgerton, Newton and More Characters. Her music resonates for those of us who have struggled to never quite feel like were at home. It was released on June 6, 1998. Who is the WandaVision ...

When you see the title you know what it is about. It is one of the most reviewed false eyelashes till date when you Google it. And I am the no.123456 blogger who actually going to share my thought on this. This Koji Dolly Wink Eye Lashes No.1 (Dolly Sweet) is my second box from Dolly Wink. My first one was the Dolly Wink Limited Edition Eyelash Set that I reviewed last year. ...

TY - JOUR. T1 - Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. AU - Lloyd, Rhiannon. AU - Lee, J.. AU - Alberio, R.. AU - Bowles, E.. AU - Ramalho-Santos, J.. AU - Campbell, K.. AU - St John, J.. PY - 2006/4. Y1 - 2006/4. N2 - Mitochondrial DNA is an extranuclear genome normally maternally inherited through the oocyte. However, the use of nuclear transfer can result in both donor cell and recipient oocyte mitochondrial DNA persisting through to blastocyst and being transmitted to the offspring. The degree of donor mitochondrial DNA transmission appears to be random and currently no evidence exists to explain this phenomenon. To determine whether this is a dilution factor or directly related to the transcriptional status of the donor cell in respect of mitochondrial DNA transcription factors, we have generated sheep nuclear transfer embryos using donor cells: (1) possessing their full mitochondrial DNA complement, (2) those partially depleted, ...

After joining the Senate in 2002, Talent supported federal legislation that would ban embryonic stem cell research or federal funding for embryonic stem cell research. This included cosponsoring a bill (S.658)[51] sponsored by Senator Sam Brownback, which would ban all forms of human cloning including embryonic therapeutic cloning techniques that are seen as crucial to stem cell research. This law was unnecessary because President Bill Clinton outlawed human cloning in 1998. On February 10, 2006, Talent withdrew his support for the bill,[52] citing the need to balance research and protection against human cloning. This move followed criticism by Talents opponent in the 2006 election, Claire McCaskill, as well as pressure from Missouri business interests that oppose restrictions on stem cell research. Though this reversal was criticized as being politically motivated,[53] Talent told the Associated Press, The technology is changing all the time and so Im always considering whether there is a ...

Medical technology has reached a point where human cloning is beginning to look like a real possibility. But is it really. And if it is, should we do it? Cloning is a form of asexual reproduction, which does not occur in nature among higher organisms. The method used is called nuclear transfer.

Medical technology has reached a point where human cloning is beginning to look like a real possibility. But is it really. And if it is, should we do it? Cloning is a form of asexual reproduction, which does not occur in nature among higher organisms. The method used is called nuclear transfer.

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

Beyond Pesticides, February 26, 2007) The United States largest dairy company, Dean Foods, has adopted a policy statement rejecting milk from cloned cows. The decision, which joins those of the organic dairy community to reject animal cloning, reflects the publics demand for foods free of genetic manipulation and chemicals.. The food giants policy comes in the midst of the Food and Drug Administrations (FDA) open comment period on its evaluation of the safety of animal cloning. While FDA is expected to determine animal products from clones are as safe as those from naturally produced animals, Dean Foods stance is a clear message that the market is not interested in purchasing them. The statement reads:. Based on the desire of our customers and consumers, Dean Foods will not accept milk from cows that have been cloned. If the FDA does approve the sale of milk from cloned cows, we will work with our dairy farmers to implement protocols to ensure that the milk they supply to Dean Foods does ...

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Using somatic cell nuclear transfer, a team of scientists in has created the first disease-specific embryonic stem cell line with two sets of chromosomes.

I need help with the following: What are the three most ethical controversies relating to stem cell research and human cloning? Why? Original work, please. Otherwise, I could have found it myself. List references, if you.

WASHINGTON (AllPolitics, Jan. 20) -- Spurred by a Chicago scientists announcement that he wants to clone a human, the federal Food and Drug Administration (FDA) says it has the authority to regulate human cloning, and will block anyone who tries it without permission. The scientist, Richard Seed, jumped into the public eye with his announcement he wants to clone a human within 18 months. Trained as a physicist, Seed has no medical degree or laboratory support, so his plan may be far-fetched. But the FDA said it can go to court to stop unauthorized cloning attempts. Were not only able to move, were prepared to move, Dr. Michael Friedman, acting FDA commissioner, told The Associated Press. One man [Seed] whos on the fringe has drawn a lot of attention in Washington and state capitals, Dr. Benjamin Younger of the American Society for Reproductive Medicine told the AP. If they are going to do this, come up with legislation that bans cloning but protects research. President Bill Clinton has ...

Free essay examples, how to write essay on Human Cloning Research Moral Organ example essay, research paper, custom writing. Write my essay on cloning human research.

The NSW Council of Churches. Churches outraged at human cloning revelations. Church groups angered by news of human cloning experiments have condemned the research outright, saying that human cloning is an affront to God and to human dignity.. It is irresponsible and immoral for Professor Severino Antinori and Professor Panayiotis Zavos to announce their expectation of success in human cloning, said Mr Ray Hoekzema, President of the NSW Council of Churches. The arrogance of their claims is unbelievable. They have declared that they intend to break the laws of nature - laws which God has put in place for our good. What they are seeking to do is an affront to God - theyre effectively saying that Gods creation, Gods choices, and Gods wisdom are not good enough for them or the rest of humanity. And just today weve heard that Australian scientists are also involved in secret cloning experiments. Where is the ethical regulation for this sort of activity? Human cloning is the grossest ...

Human cloning Many couples across the world have had problems with conceiving a child, this may have be due to the fact that they are infertile. Cloning...

If human cloning is banned in the United States it is not going to stop it from happening all it is going to do is take out of the hands of legitimate respectable scientists and put in on the street. If we ban this research it will only create a black market for human embryos with specific traits and instead of seeing the many benefits that this technology has to offer it will just make wealthy criminals richer. And if it is banned we wont be able to regulate it making those who use it even more susceptible to life threatening errors. This will only increase the loss of life that might come with this. And do you think if this research field is in the hands of crime lords they will spend their time and money doing cancer research? Of course they wont! So instead of taking the opportunity to cure one of mans worst diseases congress is busy passing legislation to grant exclusive rights of this incredible technology to criminals ...

A new stem cell discovery has reawakened controversy about human cloning - though technical challenges mean scientists are far from being able to create human babies as in Michael Bays 2005 sci-fi flick The Island.

Buried deep in an encouraging story about another advance in turning skin cells into stem cells, we see more evidence of biotechnologys ultimate human cloning goal. First, the good news. An acid bath may be able to replace viruses in

MODEL RELEASED. Human cloning. Conceptual image of identical cloned babies crawling across a DNA autoradiogram. The sequence of pink bands on the autoradiogram represents the code contained in the genetic material DNA (deoxyribonucleic acid). This determines the hereditary characteristics of an individual. Human clones are genetically-identical duplicates that are created by transplanting DNA from a template person into an egg (female reproductive cell) which has had its DNA removed. Triggering the growth and development of the egg will ultimately produce a baby that is a clone of the original individual. - Stock Image G340/0056

CES is full of some crazy stuff, but one of the craziest things we saw was from newcomer PsychaSec, which promises to grow a clone from your cells and upload your consciousness into that clone. You know, in case you die or something similarly catastrophic.When Tuan first sent photos back from the show floor, I thought someone had seriously lost their marbles. CES has its fair share of outlandish prototype tech, but human cloning is still a bit sci-fi. And I wanted to know why there wasn't a proper 'live' demo of a working clone. It also kicked off my déjà vu because I just read Richard Morgan's Altered Carbon last month, plus Down and Out in the Magic Kingdom last week. I went on a cyberpunk book binge over the holidays, and that name sounded awful familiar.

The surprising reason why human cloning may produce someone else. It turns out that we are all microchimeras with slightly different genomes in some cells.

Opponents of the stem call initiative are not accepting last weeks voter approval of it. Missourians Against Human Cloning got almost 49 percent of the voters last week to say no to Amendment Two and thats close enough for Executive Secretary Jaci Winship to vow an ongoing fight. She cautions against a quick reaction. She says a well thought-out strategy is needed for the long term, maybe a couple of years. She hopes to get a meeting of organization leaders later this week to lay out some early strategy which could include a new petition campaign to overturn the constitutional change voters approved a week ago. She says the closeness of the vote has energized people who think they did not do enough or were not involved. She says a lot of people remain confused about the issue and she sees her organization as the one to end their confusion. Legislation limiting the impact of Amendment two is likely to be introduced in the general assembly next year. Winship says several people have talked to ...

Essay Sample: Introduction: The possibility of human cloning, raised when Scottish scientists at Roslin Institute created the much-celebrated sheep Dolly, aroused

One of the most serious issues that are subject to debated is the thorny problem of human cloning due to the technological and scientific developments in our...

Why Human Cloning Is Needed - 15 Years Online. Any Complexity - Only for our Сustomers. Trial Laboratory Work - Because We are Leaders.

By 80-79 vote, General Assemblys legal committee passes motion to delay consideration of treaty banning human cloning until 2005

The Federal Circuits 12 page opinion was authored by Judge Dyk, with Judges Moore and Wallach joining. Although the claims on appeal were rejected under 35 USC §§ 101, 102 and 103, the court did not reach the issues of anticipation and obviousness, disposing of the case solely under § 101. The decision opened with a brief recap of the Supreme Court decisions in Myriad, Chakrabarty, and Funk Bros., collectively being characterized as making it clear that naturally occurring organisms are not patentable. In Funk Bros., the mixture of bacteria . . . was unpatentable because its qualities are the work of nature unaltered by the hand of man. In Chakrabarty, the modified bacterium was patentable because it was new with markedly different characteristics from any found in nature and one having the potential for significant utility. Lastly, in Myriad, claims on two naturally occurring, isolated genes . . . were invalid under § 101,″ because the genes themselves were unpatentable ...

While society debates whether and how to use public funds to support work on human embryonic stem cells (hESCs), many scientific groups and businesses debate a different question - the extent to which patents that cover ...

If you have a question about this talk, please contact Dr Xavier Moya.. Nuclear transplantation to eggs and oocytes can reprogram somatic cell nuclei from an adult pattern of gene expression to that characteristic of embryos. This is the first stage of a procedure by which replacement cells can be formed from adult cells of the same individual, thereby eliminating the need for immunosuppression. A central aim of recent work in this field is to analyze the mechanisms by which eggs and ooctyes can rejuvenate a cell from an adult to an embryonic state.. This talk is part of the Wolfson College Science Society talks series.. ...