TY - JOUR. T1 - Quantitative footprinting analysis of the chromomycin A3-DNA interaction. AU - Stankus, Allison. AU - Goodisman, Jerry. AU - Dabrowiak, James C.. PY - 1992. Y1 - 1992. N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5′-TGGCCA-3′, 3′-ACCGGT-5′ in the 18-mer with a binding constant of (2.7 ± 1.4) × 107 M-1. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is ∼ 105 M-1. Since it has been suggested that CHR binding alters DNA to the A configuration, quantitative footprinting studies using dimethyl sulfate, which alkylates at N-7 of guanine in the major groove, ...
15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004 ...
Meenakshi Priyam has joined udaan.com- a B2B trade platform, created specifically for small & medium businesses in India - as group CHRO. She has moved from GlaxoSmithKline (GSK), the pharmaceutical major, where she was CHRO for India and global HR head for classic and established products (CEP).. Priyam had spent just over three years at GSK, where she initially headed HR for India and South Asia for almost two years before being elevated to CHRO India & global HR head CEP in May last year.. Before joining GSK, Priyam had served as head HR - global product strategy & commercialisation (GPS&C) and global functions at Novartis, based out of Basel Area, Switzerland. She had joined Novartis in 2013, as head-HR, in Mumbai, before moving to Switzerland in 2016, for a year and four months.. Her longest stint was with Johnson & Johnson (J&J) where she joined as senior HR business partner in 2006. In less than three years, she worked her way up to become the Total Rewards Lead for two years and three ...
Various approaches are used to study the chromosomal makeup of cells. Traditional cytogenetic methods are based on the analysis of mitotic cells fixed onto slides to analyze their chromosomal composition (karyotype) by microscopy. This approach can be combined with FISH to detect specific sequences on morphologically distinct individual chromosomes. Disadvantages of this type of microscopic analysis are the amount of time and labor required to acquire and analyze typically less than a hundred cells. As a result, the statistical power of this type of analysis is limited. An alternative to traditional cytogenetic methods is flow karyotyping (1,2) a method to analyze chromosomes in suspension by flow cytometry. For bivariate flow karyotyping, the DNA composition of specific chromosomes in suspension is measured based on the DNA-specific dyes Hoechst 33258 and chromomycin A3 (3,4). In our protocol, we combine flow karyotyping and FISH to analyze repetitive DNA in individual chromosomes by flow ...
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1EKH: A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance.
Vikram Duggal joins as the VP and head HR for pharma solutions, while Unmesh Rai is the new head-employer branding and talent acquisition.. Pharma major Piramal Group is all set for an HR overhaul. The company now has a new CHRO in Vikram Bector, who joined a few months ago.. Following this, the company has made two new senior level appointments in HR department. Vikram Duggal has joined as the VP and head-HR for pharma solutions, and Unmesh Rai is the new head employer branding and talent acquisition.. Duggal and Rai will both work with Vikram Bector, who joined the company as the Group CHRO this July. Bector, who is driving the HR Transformation 2.0 at the Piramal Group, has vast experience in building world-class HR practices. He is well known for his expertise in leading HR transformations on a large scale; as well as for being a thought leader and a leadership coach.. Even as the Piramal Group accelerates its HR transformation through the Strategy for Employee Engagement and Development ...
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T02360 (aalt,achr,acta,actc,amyb,amyc,asw,cmos,cthd,cyl,dfn,ehl,fek,fva,hta,kak,kmx,kpnk,lei,lfb,lsh,lys,mcol,msub,mtab,noe,oor,paru,phs,pje,png,ptd,rpln,sclo,scou,seny,sera,sfz,slb,slw,snl,sphc,sphy,srub,taj : calculation not yet completed ...
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slide scanner - Lab work is more often than not left in Petri dishes and on slides, so the Ultra HD Scientific Slide Scanner is designed to help move samples to th...
1-Place a drop of nigrosin on slide, 2-Mix a loopful of broth culture with the nigrosin (no extra water is needed when the culture is taken from solid media as nigrosin contains a lot of water already), 3-Use a second slide to sweep the mixture across the first slide, making a color gradient, 4-Let the smear air dry ...
Today you are going to learn about how the digestion system functions. Read the explanation on slide 2 and answer the questions. You can also do extra research by looking at the different websites in slides 3-5 and completing the differenet activities. Record any interesting information on your white board.
You have 2 PDB codes: One that you found by searching, and one that was given to you by Imada-san and the TAs. If your found PDB code does not have ligand, give the following information on Slide 1 for two PDB codes: your found code, and your given code: ...
Streptomyces griseus subsp. griseus bacteriophage 22653 ATCC ® 11984-B1™ Designation: 22653 TypeStrain=False Application:
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Plicamycin (INN, also known as mithramycin; trade name Mithracin) is an antineoplastic antibiotic produced by Streptomyces plicatus. It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently reported in different places all with the same chromomycin core, but with different stereochemistry in the glycoside chain, a 1999 study has re-investigated the compound and proposed a revised structure. Plicamycin has been used in the treatment of testicular cancer, Pagets disease of bone, and, rarely, the management of hypercalcemia. Plicamycin has been tested in chronic myeloid leukemia. Plicamycin is currently used in multiple areas of research, including cancer cell apoptosis and as a metastasis inhibitor. One elucidated pathway shows it interacts by cross-binding chromatin GC-rich promoter motifs, thereby inhibiting gene transcription. "Mithramycin A". Fermentek. Wohlert, S. E.; Künzel, E.; Machinek, R.; Méndez, C.; Salas, J. A.; ...
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Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
Chromatin describes the complex of DNA and proteins that packs DNA into condensed structures. But this is not its only function: by packing the DNA it prevents possible transcription and therefore is a powerful mechanism for control of gene expression. Especially since the chromatin state can be passed on to progenies allowing for a fixed gene expression over multiple proliferations. Experiments in Drosophila could show that dependent on the position of gene on the chromosome and therefore its chromatin packing state different reproducible cell proliferation patterns emerged. The resulting different eye patterns lead to the hypothesis that the chromatin packaging state of a gene can govern the pattern phenotype of the corresponding tissue. Therefore chromatin engineering yields the possibility to further understand and manipulate cell patterns in mammalian cells. The basis for this project is a mammalian cell line that switches between two possible states reported by fluorescent proteins by ...
01/31/05 20:41 PM >>> I need some help!! We have been experiencing some yeast and bacteria showing up on some of our special stains. It started about 2 weeks ago and seems to be sporadic (one day...no problems, the next day, several stains are affected). The yeast and bacteria (rods) are clumped together all over the slide, on the tissue sections, around the sections and on the slide in areas where there is no tissue. The stains we have seen the contamination are the Gram, PAS/D, PAS and GMS. All of these stains are automated except the Gram, so I do not think the special stainer is the problem. Here is what I have eliminated as problem areas: -Processor solutions and paraffin (we cut and stained a block from another institute and had contamination) -Waterbaths, slide drying boards, water containers (used to fill waterbathes), stainer containers, ice pans (all of the mentioned have been thoroughly bleached) -Tap water and DI water (all water supplies have been tested by sending a sample to ...
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Ions as a source of reactants: Ions get neutralized when they reach the surface, they become an additional source of reactive species.. The synergy between ion bombardment and chemical etching was first shown by Coburn and Winters in the classical experiment shown on slide 2.. Besides enhancing the chemical etch, ions also play a major role in removing non-volatile by-products or etch products that require an activation energy to desorp from the surface. The removal of by-products and their redeposition onto the feature sidewall is the fundamental reason why plasma etching can obtain anisotropic profiles (slide 3). Factors that influence the anisotropy are (slide 4 ...
Disclosed is an automated staining apparatus including an arm moveable in three dimensions, and a hollow tip head located on the arm including integral reagent tip head, wash tip and blow tip for selectively dispensing gas and liquid onto microscope slides. Also disclosed are various sub-components of the apparatus that are specifically adapted to the processing of specimens on slides.
... is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
Cancer can often be detected in the arrangement of cells in a tissue sample. Once a sample tissue is taken from the patient, it is sent to the histotechnician (HT), who prepares the tiny sections of body tissues for microscopic examination by a pathologist. Working closely with the pathologist, the histologic technician processes tissue biopsies removed during surgery. The tissue is cut into very thin slices, mounted on slides and stained with special dyes to make the cell details visible under the microscope. By examining the section of tissue, the pathologist and the surgeon can learn if disease is present, or if it has spread, and decide the best course of treatment for the patient. The histotechnologist (HTL) has advanced training in how and why specimens are collected and processed for testing. That expertise qualifies the histotechnologist to manage even unexpected situations in the laboratory, such as solving technical or instrument problems, understanding the underlying health and ...
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Even relatively minor errors in chromatin remodeling during spermiogenesis are associated with sperm DNA damage and infertility, yet little is known about the etiology. Mice with severe NPYq deletions are infertile due to severe sperm differentiation defects (Ward and Burgoyne, 2006; Yamauchi et al., 2009). We have recently observed that sperm from these mice presented abnormal chromatin packaging and DNA damage. Moreover, when these sperm were injected into the oocytes, a significant increase of oocyte arrest at pronuclei stage and of chromosome aberrations in the fertilized eggs were noted (Yamauchi et al., 2010). Here we provide evidence that the deficiency of NPYq encoded gene Sly is associated with sperm DNA damage and poor sperm chromatin condensation, and propose that SLY plays a role in spermatid-specific chromatin remodeling.. How can Sly/SLY be involved in sperm DNA damage phenotype? SLY protein has been shown to control the postmeiotic expression of ,100 genes, the majority of which ...
Euphorbia Linnaeus, 1753 (Euphorbiaceae) is one of the most diverse and complex genera among the angiosperms, showing a huge diversity in morphologic traits and ecologic patterns. In order to improve the knowledge of the karyotype organization of Euphorbia hirta (2n = 18) and E. hyssopifolia (2n = 12), cytogenetic studies were performed by means of conventional staining with Giemsa, genome size estimations with flow cytometry, heterochromatin differentiation with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and Giemsa C-banding, fluorescent in situ hybridization (FISH) with 45S and 5S rDNA probes, and impregnation with silver nitrate (AgNO3). Our results revealed small metacentric chromosomes, CMA+/DAPI0 heterochromatin in the pericentromeric regions of all chromosomes and CMA+/DAPI− in the distal part of chromosome arms carriers of nucleolar organizing regions (NORs). The DNA content measurements revealed small genomes for both species: E. hirta with 2C = 0.77 pg and E. hyssopifolia
ID B1VKL9_STRGG Unreviewed; 119 AA. AC B1VKL9; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 18-JUL-2018, entry version 26. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG16864.1}; GN OrderedLocusNames=SGR_35t {ECO:0000313,EMBL:BAG16864.1}, SGR_7104t GN {ECO:0000313,EMBL:BAG23931.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG16864.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}, and RC NBRC 13350 {ECO:0000313,EMBL:BAG16864.1}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT "Genome sequence of the ...
Eukaryotic DNA is packaged in chromatin, whose repeating subunit, the nucleosome, consists of an octamer of histone proteins wrapped by about 147bp of DNA. This packaging affects the accessibility of DNA and hence any process that occurs on DNA, such as replication, repair, and transcription. An early observation from genome-wide nucleosome mapping in yeast was that genes had a surprisingly characteristic structure, which has motivated studies to understand what determines this architecture. Both sequence and trans acting factors are known to influence chromatin packaging, but the relative contributions of cis and trans determinants of nucleosome positioning is debated. Here we present data using genetic approaches to examine the contributions of cis and trans acting factors on nucleosome positioning in budding yeast. We developed the use of yeast artificial chromosomes to exploit quantitative differences in the chromatin structures of different yeast species. This allows us to place approximately 150kb
Hydrocortisone reveals its action by activation of transcription of specific genes that is achieved by binding of hormones nuclear receptors (NRs) to cognate sites on DNA and recruiting coactivators of basal transcription machinery. NRs are able to overcome restricting conformation of chromatin by altering chromatin-remodeling machinery (chromatin-remodeling protein complexes, histone and nonhistone modifying enzymes). However, a possibility arise that genomic DNA accessibility to NRs as well as chromatin packaging pattern in nuclei implicates complex processes which involve regulation of DNA modifying enzymes activities.. Whether hydrocortisone induced decondensation of chromatin alter DNA accessibility in nuclei upon exogenously applied DNAse I and cause eventual changes in endogenous Ca-Mg endonuclease-depended fragmentation become a focus of our research.. Experiments were conducted in vivo. Hydrocortisone 5 mg/100g body wt was administrated by intraperitonial injection. Hormone treated and ...
TY - JOUR. T1 - pRb2/p130 and p107 control cell growth by multiple strategies and in association with different compartments within the nucleus. AU - Zini, Nicoletta. AU - Trimarchi, Carmela. AU - Claudio, Pier Paolo. AU - Stiegler, Peter. AU - Marinelli, Fiorenzo. AU - Maltarello, Maria Cristina. AU - La Sala, Dario. AU - De Falco, Giulia. AU - Russo, Giuseppe. AU - Ammirati, Giuseppe. AU - Maraldi, Nadir Mario. AU - Giordano, Antonio. AU - Cinti, Caterina. PY - 2001. Y1 - 2001. N2 - It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, with nuclear compartmentalization of retinoblastoma proteins. In a first series of experiments, we showed that pRb2/p130 and p107 are not evenly distributed within the ...
The repair of DNA damage in highly compact, transcriptionally silent heterochromatin requires that repair and chromatin packaging machineries be tightly coupled and regulated. KAP1 is a heterochromatin protein and co-repressor which binds to HP1 during gene silencing, but is also robustly phosphorylated by ATM at serine 824 in response to DNA damage. The interplay between HP1-KAP1 binding/ATM phosphorylation during DNA repair is not known. We show that HP1α and unmodified KAP1 are enriched in endogenous heterochromatic loci and at a silent transgene prior to damage. Following damage, γH2AX and pKAP1-s824 rapidly increase and persist at these loci. Cells which lack HP1 fail to form discreet pKAP1-s824 foci after damage but levels are higher and more persistent. KAP1 is phosphorylated at Serine 473 in response to DNA damage and its levels are also modulated by HP1. Unlike pKAP1-s824, pKAP1-s473 does not accumulate at damage foci but is diffusely localized in the nucleus. While HP1 association ...
Objective: The objective of this selective EBM review is to determine whether or not dasatinib improves outcomes and tolerability in patients with chronic myeloid leukemia as compared to imatinib. Study Design: Review of three English language, non-blinded randomized controlled trials from 2009, 2010, and 2010. Data Sources: Randomized, controlled, non-blinded clinical trials comparing dasatinib to imatinib or comparing dasatinib once daily vs dasatinib twice daily, found using the PubMed database. Outcomes measured: Overall survival and progression-free survival were measured at one and two years after initiation of therapy. Safety profiles and incidence of adverse effects were also measured. This is graded on a scale of 1 to 4, from lowest in severity to highest in severity. Additionally, adverse effects were noted as hematologic (neutropenia, anemia, thrombocytopenia) or nonhematologic (fluid retention, diarrhea, vomiting, fever). Results: When comparing dasatinib to imatinib, both drugs provided
A new Korn Ferry (NYSE:KFY) survey of Chief Human Resource Officers (CHROs) shows that as the HR function becomes more strategic and high-profile, HR
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Chronic Kidney Disease (CKD) is divided into 5 stages from stage 1 to stage 5. If you have kidney disease, your kidneys are slowly losing their ability to remove wastes and excess water from your blood3 kidney disease indicates moderate chro
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Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
VivoTag 680 XL Fluorochrome ideal for labeling nanoparticles and macromolecules. Hydrolytically stable and low self quenching for higher loading applications.
Tekutý kokosový olej na varenie, pečenie, kozmetické účely, antibakteriálny výplach ústnej dutiny, zlepšuje metabolizmus a chudnutie, znižuje cukor
Synonyms for aureolic acid in Free Thesaurus. Antonyms for aureolic acid. 1 synonym for mithramycin: Mithracin. What are synonyms for aureolic acid?
Figure 3 - Phenogram of overall molecular similarity of RAPD bands among specimens from all localities at Tibagi, Paraná, and Iguaçu Rivers. Codes as in Figure 1. DISCUSSION The RAPD-PCR patterns indicate strong genetic differentiation within and among populations in the Paraná and the Tibagi Rivers. In the Paraná River at Porto Rico, the high levels of allelic polymorphism may represent the pristine composition of different species or populations within the H. malabaricus species complex, or they may be the outcome of faunal mixing caused by the closing of the Itaipu Dam in 1978 and the consequent flooding of Sete Quedas, another Oligocenic geographic barrier (Sampaio, 1988). Sete Quedas was the natural boundary for the Upper and Middle Paraná basins. The presence of an outlier in the Paraná River similar to the Iguaçu population suggests that the Paraná may indeed be a recipient of trahiras of diverse origins. The Londrina sample from the Tibagi River is most similar to the populations ...
A method is described in which smears on slides, which had been examined previously in a direct fluorescence antibody (DFA) test for Chlamydia trachomatis, were tested by the polymerase chain reaction (PCR). Twenty four (73%) of 33 smears which contained fewer than 10 elementary bodies when examined by the DFA test were positive by the PCR. Of the nine negative smears, seven contained only one or two elementary bodies. However, single elementary bodies were detected by the PCR in seven of the 24 positive samples. Fifteen smears were negative by both methods. The ability to detect small numbers of elementary bodies by the PCR and its specificity for negative smears indicates its potential for retrospective analysis of stored, archival smears on slides.. ...
ID B1VQ72_STRGG Unreviewed; 299 AA. AC B1VQ72; DT 20-MAY-2008, integrated into UniProtKB/TrEMBL. DT 20-MAY-2008, sequence version 1. DT 10-APR-2019, entry version 33. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:BAG17173.1}; GN OrderedLocusNames=SGR_344 {ECO:0000313,EMBL:BAG17173.1}; OS Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350). OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; OC Streptomyces. OX NCBI_TaxID=455632 {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685}; RN [1] {ECO:0000313,EMBL:BAG17173.1, ECO:0000313,Proteomes:UP000001685} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JCM 4626 / NBRC 13350 {ECO:0000313,Proteomes:UP000001685}; RX PubMed=18375553; DOI=10.1128/JB.00204-08; RA Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H., RA Yamashita A., Hattori M., Horinouchi S.; RT "Genome sequence of the streptomycin-producing microorganism RT Streptomyces griseus IFO 13350."; RL J. Bacteriol. ...
Eukaryotic organisms package DNA into chromatin for compact storage in the cell nucleus, but this packaging process results in transcriptional repression of genes. Chromatin remodeling complexes have evolved to overcome the transcriptional repression caused by chromatin packaging of DNA into nucleosomes by histones. One example of a chromatin remodeling complex is the SWI/SNF complex in yeast which uses ATP to drive the chromatin apart and make DNA accessible to transcription factors. The yeast SWI2 protein was discovered as the catalytic subunit of the yeast SWI/SNF chromatin remodeling complex and is required for the complex to counteract the repressive nature of chromatin. BRG1 and BRM, SWI2 homologs, are part of human chromatin remodeling complexes and have been shown to play a redundant role in the regulation of certain cell cycle and cellular adhesion genes, as well as cellular pathways. Recent studies showing loss of BRG1 in human tumor cell lines and primary tissue samples, BRG1 ...
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