BACKGROUND - Chromogranin A, coreleased with catecholamines by exocytosis, is cleaved to the catecholamine release-inhibitory fragment catestatin. We identified a natural nonsynonymous variant of catestatin, Gly364Ser, that alters human autonomic function and blood pressure. METHODS AND RESULTS - Gly364Ser heterozygotes and controls underwent physiological and biochemical phenotyping, including catecholamine production, chromogranin A precursor, and its catestatin product. Case-control studies replicated effects of the gene on blood pressure in the population. Gly364Ser displayed diminished inhibition of catecholamine secretion from cultured neurons. Gly/Ser heterozygotes displayed increased baroreceptor slope during upward deflections (by ≈47%) and downward deflections (by ≈44%), increased cardiac parasympathetic index (by ≈2.4-fold), and decreased cardiac sympathetic index (by ≈26%). Renal norepinephrine excretion was diminished by ≈26% and epinephrine excretion by ≈34% in Gly/Ser ...
Heritable autonomic traits may be of particular value in investigations of the genetic underpinnings of hypertension, a complex trait with both hereditary and environmental determinants and likely even multiple genetic (or "polygenic") determinants.7,8 Human twin studies indicate that baroreceptor activity is a substantially heritable trait.33 Such traits may be valuable "intermediate phenotypes"7,8 for hypertension, especially if they display earlier penetrance than the ultimate disease trait (DBP), can be elicited in still-normotensive individuals, and suggest testable candidate genetic loci (such as CHGA). Catestatin itself may be such a phenotype.8 Several of the autonomic phenotypes studied here (Table II and Figures 2 and 3⇑) meet these criteria.. Indeed, Gly364Ser genotypes were associated with hypertension (Figure 4a and 4b), especially in men (Figure 4b). Thus, the 364Ser allele may play a protective role against future the development of hypertension.. How might this happen? Because ...
Naturally Occurring Human Genetic Variation in the 3′-Untranslated Region of the Secretory Protein Chromogranin A Is Associated With Autonomic Blood Pressure Regulation and Hypertension in a Sex-Dependent Fashion Academic Article ...
The chromogranins/secretogranins are a family of acidic, soluble proteins with widespread neuroendocrine distribution in secretory vesicles. Although the precise function of the chromogranins remains elusive, knowledge of their structure, distribution, and potential intracellular and extracellular roles, especially that of chromogranin A, has greatly expanded during recent years. Chromogranin A is coreleased with catecholamines by exocytosis from vesicles in the adrenal medulla and sympathetic nerve endings. Thus, measurement of its circulating concentration by radioimmunoassay may be a useful probe of exocytotic sympathoadrenal activity in humans, under both physiological and pathological conditions. Here, we explore the storage, structure, and function of chromogranin A, and parameters that influence its circulating levels. We have also measured plasma chromogranin A concentrations in different groups of patients with hypertension, including those with pheochromocytoma. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The structure of the gene encoding bovine chromogranin-A has been determined by characterization of two isolated genomic clones. Chromogranin-A is encoded by eight exons, which organize the coding region into several distinct structural and functional domains. Exons 1-5 represent the highly conserved signal peptide and N-terminal domain, which are separated into regions corresponding to the signal peptide, N-terminal sequence, disulfide-bonded loop, and remainder of the conserved N-terminal domain. Exon 6 represents the variable domain and encodes a region that is identical to the novel chromogranin-A-derived peptide chromostatin. Exon 7 encodes the biologically active peptide pancreastatin as well as most of the conserved C-terminal domain, with the remainder found on exon 8. The mRNA sequence obtained from the gene contains five nucleotide differences from the consensus sequence of four reported bovine chromogranin-A cDNA clones. Two of the differences in the gene result in two amino acid changes in
Chromogranin A is a secretory protein, composed of 439 amino acids, found in the large dense-core vesicles of the neuroendocrine cells. It belongs to the family of granins that includes chromogranin B, chromogranin C, and secretogranin II.
Chromogranin A / CHGA (Neuroendocrine Marker) Antibody - Without BSA and Azide, Mouse Monoclonal Antibody [Clone SPM553 ] validated in IHC-P, IF, FC (AH10405-100), Abgent
Chromogranin A / CHGA (Neuroendocrine Marker) Antibody - With BSA and Azide, Purified Mouse Monoclonal Antibody validated in WB, IHC, IF, FC, IP (AH10256-20), Abgent
Chromogranin A (CgA) levels have previously been found to predict mortality in heart failure (HF), but currently no information is available regarding CgA processing in HF and whether the CgA fragment catestatin (CST) may directly influence cardiomyocyte function.. ...
ntroduction: Serum levels of Chromogranin A (CgA) were measured in consecutive patients with prostate diseases in order to evaluate the impact of age on CgA diagnostic significance. Materials and Methods: Serum levels of CgA were determinated in 217 consecutive patients immediately before prostate biopsy: CgA differences between cases (prostate cancer PC) and control (benign prostatic hyperplasia BPH) were analyzed, and CgA performance in prediction of PC was compared with age and standard diagnostic tools. CgA values were also analyzed in patients affected by PC, and compared with age and standard prognostic parameters. Results: At multivariate analysis, CgA approaches a statistically significant value as independent predictor of PC and positively correlates with age. In PC group, CgA positively correlates with age, while no correlations are found with PSA, Gleason score or stage of disease. Conclusions: Age, correlating with CgA values in overall population and in PC subgroup, emerged as a ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Purpose: To investigate the distribution of the novel chromogranin A-derived neuropeptide serpinin in the rat eye.. Methods: The molecular form of serpinin-immunoreactivity detected by a highly specific antiserum was evaluated both by western blot analysis and reversed phase-HPLC with subsequent ELISA in the rat trigeminal ganglion (TG) and retina and the presence and distribution of the peptide was explored in the rat TG and eye by immunofluorescence.. Results: There was no band in western blots in the rat TG but two weak bands at 30 and 50 kDa in the retina. Only free serpinin but no (pGlu)-serpinin and no serpinin-RRG were detected by RP-HPLC in the rat TG and retina. Numerous small- and medium-sized cells contained serpinin-immunoreactivity in the rat TG and full colocalization with substance P was evident with a more widespread expression of serpinin. Immunoreactivity was present in nerves in the corneal, limbal, irideal, ciliary and choroidal/scleral stroma, sphincter and dilator muscle ...
Pituitary secretory protein I, CgA, Chromogranin A precursor, SP-I, CGA, chromogranin A (CgA), CHGA, chromogranin A (parathyroid secretory protein 1 ...
Product Name: Chromogranin A antibody Applications: IHC-PPredicted Target Size: Positive Controls: Form Supplied: LiquidConcentration: Purification: Ab purified
Mouse monoclonal antibody raised against full length recombinant human CHGA. Recombinant protein corresponding to full length human CHGA. (MAB14233) - Products - Abnova
NE differentiation in pancreatic carcinomas has been reported in various studies using IHC (13, 14, 15) . However, the expression of PGP9.5 and its relation to other NE markers have not been studied in pancreatic cancer to date. In this study, CGA was used as a marker for NE differentiation because it has been demonstrated to be a well-characterized marker for NE differentiation (16) . We found that tumor cells immunoreactive to CGA monoclonal antibody accounted for ,1% of the tumor cell population. On the other hand, PGP9.5 was expressed in 26 of 69 (37.7%) pancreatic cancers. These results suggested that the expression of PGP9.5 in pancreatic cancer is independent of NE differentiation.. Subsequently, we investigated a possible correlation of PGP9.5 status with clinicopathological features. Although PGP9.5 expression was not associated with tumor histology or the extent of the tumor, pancreatic cancer patients with PGP9.5 expression had significantly shorter survival times than those without ...
Mouse Monoclonal Anti-Chromogranin A Antibody (SPM553) [DyLight 650]. Neuroendocrine Marker. Validated: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP. Tested Reactivity: Human. 100% Guaranteed.
Mouse Monoclonal Anti-Chromogranin A Antibody (PHE5) [Alexa Fluor® 700]. Neuroendocrine Marker. Validated: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: Human, Primate, and more. 100% Guaranteed.
Neuroblastoma (NB) is a paediatric form of cancer derived from the sympathetic nervous system. Recent genome-wide sequencing data suggest that often NB does not have a clear genetic cause, leading the authors to hypothesize that NB results from aberrations of normal development. To test this hypothesis, Anna Philpotts group used a population of anteroventral noradrenergic (AVNA) cells from Xenopus embryos. These cells share several features with mammalian sympathetic neurons, including the expression of noradrenergic-associated genetic markers such as the achaete-scute complex-like 1 (Ascl1) gene, which encodes a transcriptional driver of neurogenesis. By comparing AVNA and NB cells, the authors found that, whereas Ascl1 is only transiently expressed in AVNA cells, it is aberrantly maintained in NB, where it is phosphorylated on multiple serine-proline sites. The authors then show that differentiation of AVNA cells is enhanced by dephosphorylated Ascl1. Moreover, this process is inhibited by ...
Angiogenesis has been postulated to be critical for the pathogenesis of multiple myeloma (MM), a neoplastic disease characterized by abnormal proliferation of malignant plasma cells in the bone marrow (BM). Cleavage of the N- and C-terminal regions of circulating chromogranin A (CgA, CHGA), classically an anti-angiogenic protein, can activate latent anti- and pro-angiogenic sites, respectively. In this study, we investigated the distribution of CgA-derived polypeptides in MM patients and the subsequent implications for disease progression. We show that the ratio of pro-/anti-angiogenic forms of CgA is altered in MM patients compared with healthy subjects, and that this ratio is higher in BM plasma compared with peripheral plasma, suggesting enhanced local cleavage of the CgA C-terminal region. Enhanced cleavage correlated with increased VEGF and FGF2 BM plasma levels and BM microvascular density. Using the Vk*MYC mouse model of MM, we further demonstrate that exogenously administered CgA was ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
In development of colorectal cancer (CRC), stem cell (SC) overpopulation underlies tumor initiation and progression, but it is incompletely understood which dysregulated mechanisms cause SC overpopulation. Because neuroendocrine cell (NEC) and SC populations both reside in the SC niche at the normal crypt bottom, we hypothesized that i) NECs play a role in maintaining the SC population, and ii) changes in the NEC population contributes to SC overpopulation and altered epithelial: stromal interactions during tumorigenesis. Immunofluorescence mapping and flow cytometry were used to analyze SC niche structure and cell-cell interactions in normal and neoplastic colonic tissues from Caucasian and African-American patients. In normal colonic crypts, most cells (77%) staining positively for the colonic SC marker ALDH1, also co-stained for the NE cell marker chromogranin-A (CGA). In the progression from normal to normal-appearing FAP to adenomatous to malignant colonic tissue, the proportion of ...
Farging immunhistokjemiske analyser (ER, Synaptophysin, Vimentin, E-cadherin (CDH1), CEA mono, Programmed death ligand 1 (PDL1), ALK D5F3, CD5, CD23, CD30, CD117, PAX8, PSA, S100, CD31, CD34, CDX2, CK5/6, CK20, CK-PAN (MNF116), CyclinD1, Desmin, Ki67, p40, SOX10, TTF1, Chromogranin A (CGA), Melan-A, HER-2 IHC, CK14, CD3, CD20, CD10, CK7, p16, p53, IgL - Kappa/ IgL - Lamda ...
Immunohistochemistry, is a special staining process performed on tissue removed during biopsy. Immunohistochemistry for lung cancer represents an important complementary tool for the routine diagnosis of lung cancer and for the identification of the different histological types and prognostic factors. We offer panel of IHC markers: TTF 1, p63, Ck 7, Ck 5/6, Napsin, Chromogranin and Ck 20.. ...
This article discusses the importance of testing for Chromogranin A levels in a variety of tumors, especially in the adrenal gland. Learn how this test works and what the results mean.
Not the biggest profit for over 2k BCs but... This guy is in the same system as me so it took very little deut to fly, not to mention only under 15 minutes ther
Ive been busy lately but I havent been inactive so I post the CRs that are days old. To be honest Im not particularly proud of this one because Ive sent th