Results: The retention time was observed at 2.718 min. The system suitability results were found to be within limits. The method was precise, with lower than 2 %RSD and the calibration curve was linear (r2=1) over a concentration range of 2.5-160 µg/ml. The detection and quantification limit was found to be 0.045 µg/ml and 0.15 µg/ml, respectively. Recovery of the drug was found between 100.09-100.19%. The assay of ropinirole hydrochloride in ROPITOR® and ROPARK® tablets were found to be 100.4 and 103.60 %, respectively. The forced degradation studies were carried out to demonstrate the specificity of the method by exposing the API under conditions of hydrolysis, oxidation, thermal and photolytic as per ICH Q1A(R2) guidelines. ...
A simple and precise stability indicating method for the simultaneous estimation of Dapagliflozin and Saxagliptin in combined tablet dosage form was developed and validated using RP-HPLC. The chromatographic separation of the drugs was achieved with a Xterra C-18 analytical column (150 mm × 4.6 mm i.d., part
A simple rapid stability-indicating RP-HPLC assay method was developed and validated for quantitative determination of lidocaine hydrochloride in bulk..
Reversed-phase liquid chromatography (RPLC) is a widely used technique for analytical separations but routinely requires empirical optimization. Gaining a better understanding of the molecular reasons for retention may mean more efficient separations with fewer trial and error runs to obtain optimized separations. Vibrationally resonant sum frequency generation (VR-SFG) is a surface specific technique that has allowed for in situ examination of model RPLC stationary phases under various solvent and pressure conditions. In order to improve on past work with model RPLC stationary phases two challenges had to be overcome. First, improved vibrational mode assignments of the C18 stationary phase were needed for proper understanding of this model system. Second, the synthesis of back-surface reference mirrors used in these VR-SFG experiments allowed us to better correct the relative intensities of the various spectral peaks present in typical spectra. After examination of model RPLC systems under various
Inability of UV spectrophotometric and RP-HPLC methods to perform as stability indicating assay method for the quantification of eugenol
Patta Salomi*, S. Rama Thulasi, K. Ravindra Reddy P. Rami Reddy Memorial College of Pharmacy, Kadapa-516003, Andhra Pradesh, India ABSTRACT A simple reverse phase liquid Chromatographic method has been developed and subsequently validated for determination of Brivaracetam. The mobile composition of Potassium Dihydrogen orthophosphate (0.02M): Methanol 40:60v/v Buffer pH 6.0 adjusted with ortho phosphoric acid, waters column of C18 (250X4.6 ... Read More ». ...
Perform forced degradations analysis on drug formulations and manufactured products including hydrolysis (acid and base catalyzed), thermal, photolysis and oxidation using LC/UV/MS ...
M. Lakshmi Prasanna*, C.S. Parameswari, B.V. Ramana, G. Nagarajan Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, Andhra Pradesh, India A B S T R A C T The aim present research work to development and validation of RP-HPLC method for the simultaneous estimation of Glecapravir and Piberentasvir. Chromatographic separation was evaluated by Xterra C18 column (250 X 4.6 mm, ... Read More ». ...
In the setup used in the case study (100 µL protein A column at 12 s residence time and Superdex 200 Increase 10/300), the biggest limitation lies in the 500 µl collected from the protein A column. When higher amounts were eluted from the protein A step, we lost protein as part of the peak was not collected. A solution would be to find a SEC column, onto which larger volumes can be injected, while keeping a good resolution. Increasing the residence time to, for example, 30 s will also increase the yield but triple the run time of the protein A step and consequently reduce the number of samples that can be processed. You also have to keep in mind that one should not load more than 1 mg on the Superdex 200 Increase 10/300 column.. ...
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A simple, specific, accurate, and stability-indicating RP-HPLC method was developed and validated for the simultaneous determination of Trimethoprim (TMP) and Sulfadimethoxine sodium (SDMS) in Vetricine® oral solution product. The desired separation was achieved on an ODS column (250×4.6 mm i.d., 5 μm) at room temperature. The optimized mobile phase consisted of an isocratic solvent mixture of water:acetonitrile:triethylamine (700:299:1, v/v/v), adjusted to a pH of 5.7 ± 0.05 with 0.2N acetic acid. The mobile phase was fixed at 0.8 ml/min and the analytes were monitored at 254 nm using a photodiode array detector. The effects of the chromatographic conditions on the peaks USP tailing factor, column efficiency, and resolution were systematically optimized. Forced degradation experiments were carried out by exposing TMP, SDMS standards, and the oral solution formulation to thermal, photolytic, oxidative, and acid-base hydrolytic stress conditions. The degradation products were well-resolved from the
Development and Validation of Stability-indicating RP-HPLC Method for The Estimation of Pseudoephedrine, Ambroxol and Desloratadine in Bulk and Their Tablet Dosage Forms, N. Mallik
TY - JOUR. T1 - Determination of transdermal sildenafil in nude mouse skin by reversed-phase high-performance liquid chromatography. AU - Liaw, Jiahorng. AU - Chang, Ting Wei. PY - 2001/12/25. Y1 - 2001/12/25. N2 - A simple and sensitive high-performance liquid chromatographic method was developed for the determination of sildenafil transdermal permeation of nude mouse skin. A reversed-phase column with UV detection at 224 nm was used for chromatographic separation. The mobile phase consisted of 32% acetonitrile with 0.2% phosphoric acid in water at pH 5.3 adjusted with 10 M NaOH with the flow-rate set at 1.0 ml/min. The limit of quantitation achieved was 5 ng/ml, and the calibration curve showed good linearity over the concentration range of 5-500 ng/ml. The relative standard deviations of within- and between-day analyses were all within 15%. Sildenafil was found to be stable between pH 3 and 12 during 24-h incubation with skin. After transdermal administration of 15.8 μg/ml of sildenafil to ...
A dual retention combined with reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) has been observed on beta-cyclodextrin (beta-CD) bonded stationary phase. A typical U-shaped retention curve was achieved owing to dual retention mechanism Based on this observation, a beta-CD column can be operated under reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) modes. Two-dimensional liquid chromatography (2D-LC) analysis can be realized on Just a beta-CD column by switching these two different separation modes. In this study, off-line 2D-LC analysis for a natural product was carried out to prove the orthogonal separation between RP-LC and HILIC modes on a Click beta-CD column. Herba Hedyotis Diffusae, the whole grass of Hedyotis Diffusae wild was extracted with water, pretreated with macroporous resin and then first separated at RP-LC mode on the Click beta-CD column to obtain successive fractions, which were then ...
Pristinamycin is an antibiotic used mainly in the treatment of Staphylococcus infections. The aim of this study was to develop a rapid and simple stability-indicating RP-HPLC method for the determination of pristinamycin in tablet dosage form. Pristinamycin was eluted on the ACE-5, C18-HL, 250 x 4.6 mm, 5 µm analytical column with a mobile phase consisting of 0.2% orthophosphoric acid and acetonitrile 63:37 v/v, pumped at 1.5 ml/min flow rate. The column was maintained at 40°C and 10 μl of the solutions were injected. UV detection was performed at 206 nm. The procedure separated pristinamycin and its potential degradation products in an overall analysis time of less than 10 min with pristinamycin eluting at about 3 min. The method was validated according to the regulatory guidelines with respect to specificity, precision, accuracy, linearity, and robustness. Forced degradation studies were also performed for pristinamycin bulk drug samples to demonstrate the stability-indicating power of the HPLC
P.L. Desbène, F.I. Portet, G.J. Goussot. Quantitative trace analysis of surfactant mixtures by reversed-phase high-performance liquid chromatography with refractometric detection. Journal of Chromatography A, Elsevier, 1996, 730 (1-2), pp.209 - 218. ⟨10.1016/0021-9673(95)01087-4⟩. ⟨hal-01936813⟩ ...
Alfa Aesar™ Potassium dihydrogen citrate hydrate, 99% (dry basis) 500g Alfa Aesar™ Potassium dihydrogen citrate hydrate, 99% (dry basis)...
The objective of the current study is to develop a validated specific stability-indicating isocratic reversed-phase liquid chromatographic method for the quantitative determination of levofloxacin and its related substances in pharmaceutical dosage forms in the presence of degradation products and its process-related impurities. Forced degradation studies were performed on levofloxacin as per the International Conference on Harmonisation (ICH)-prescribed stress conditions using acid, base, oxidative, water hydrolysis, thermal stress and photolytic degradation to show the stability-indicating power of the method. Significant degradation was observed during oxidative stress; minor degradation was observed in acidic stress and no degradation was observed in other stress conditions. The chromatographic method was optimized using the samples generated from forced degradation studies and the spiked impurity solution. The analysis was carried out with a 50 mm length × 4.6 mm i.d., 3.0 μm particle ...
The present study was proposed to develop a simple, rapid, and economical UHPLC method was described for the method development, validation and forced degradation for the simultaneous estimation of glycopyrrolate and formoterol fumarate in bulk drug. The chromatographic conditions were observed in the column using Zorbax RXC18 (150 × 4.6 mm) 5 µm using a […]
Determination of free urinary cortisol is a test of choice in the diagnosis of Cushings syndrome. In this study, cortisol was quantified using reversed-phase high-performance liquid chromatography (RP-HPLC) in urine samples previously extracted with ether and using triamcinolone acetonide as internal standard (IS). A BDS-Hypersil-C18® column, water-acetonitrile (72:28; v/v), with a flow rate of 1.0 mL/min and detection at 243 nm were used. This method showed to be both effective and efficient, with sensitivity and linearity ranging from 2.50 to 150 μg/L, and can be used in substitution to the radioimmunoassay technique within this concentration range ...
Mutalik, S., Hewavitharana, A., Shaw, P. N., Anissimov, Y. G., Roberts, M. S. and Parekh, H. S. (2009) Development and validation of a reversed-phase high-performance liquid chromatographic method for quantification of peptide dendrimers in human skin permeation experiments. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877 29: 3556-3562. doi:10.1016/j.jchromb.2009.08.039 ...
Quality by design approach for simultaneous estimation of doxycycline hyclate and curcumin by RP-HPLC method, C Dhal1, FJ Ahmad1, A Chauhan2, M Jyothi2, RM Singh2, PK Saini2, SC Ma
This paper reports the utilization of 2-pyrrolecarboxaldehyde-4-phenylsemicarbazone (PPS) as a complexing reagent for the simultaneous determination and separation of Ni(II), Cu(II), Pd(II), and Ag(I) by reversed-phase high-performance liquid chromatography with UV detector. A good separation was achieved using Microsorb C18 column (150 × 4.6 mm i.d.) with a mobile phase consisted of methanol : acetonitrile : water : sodium acetate (1 mM) (68 : 6.5 : 25 : 0.5 v/v/v/v) at a flow rate of 1 mL/min. The detection was performed at 280 nm. The linear calibration range was 2–10 |i|μ|/i|g/mL for all metal ions. The detection limits (S/N = 3) were 80 pg/mL for Ni(II), 0.8 ng/mL for Cu(II), 0.16 ng/mL for Pd(II), and 0.8 ng/mL for Ag(I). The applicability and the accuracy of the developed method were estimated by the analysis of Ni(II) in hydrogenated oil (ghee)
A simple, rapid and precise reverse phase liquid chromatographic (RP-HPLC) method was developed and subsequently validated for simultaneous estimation of Metformin hydrochloride and Telmisartan in bulk drug and in a synthetic mixture. The analysis was carried out using Zodiac ODS C18 (4.6 x 250mm, 5μm, Make: Zodiac Life Sciences), pre-packed column. The separation was carried out using a mobile phase containing a buffer of pH 4.0, Acetonitrile and Methanol (30:30:40 v/v/v), was pumped at a flow rate of 1.4 mL/min with UV-detection at 228 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 2.523 minute for Metformin hydrochloride and 5.437 minute for Telmisartan. The method was validated and shown to be linear for Metformin hydrochloride and Telmisartan. The correlation coefficients for Metformin and Telmisartan are 0.996 and 0.998 respectively.
China Monopotassium Phosphite 98%, Find details about China Potassium Dihydrogen Phosphite, Mono Potassium Phosphite from Monopotassium Phosphite 98% - Changsha Green Mountain Chemical Co., Ltd.
A simple and reliable reversed-phase high-performance liquid chromatographic (HPLC) method for the routine determination of vitamins A and E and β-carotene in plasma (or serum) with wavelength-programmed ultraviolet-visible absorbance detection is described. A 200-μl aliquot of serum or plasma sample, after deproteinization with ethanol, and containing tocopherol acetate as internal standard, was extracted with butanol-ethyl acetate. Sodium sulphate was added for dehydration. Analytes of extracted samples were found to be stable for at least four days. A 10-μl aliquot of this organic extract was used for HPLC analysis. The mobile phase was methanol-butanol-water (89.5:5:5.5, v/v) and the flow-rate was set at 1.5 ml/min. The analytes of interest were well separated from other plasma constituents within 22 min at 45°C. The lowest detection limits of vitamins A and E and β-carotene were 0.02, 0.5 and 0.1 μg/ml, respectively. The recovery and reproducibility of the present method were around ...
A simple reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine and Olmesartan in bulk and tablet form. Chromatography was performed by isocratic reverse phase separation on a stainless steel column 4.6 x 150mm, symmetry column packed with octa decyl silane bonded to porous silica (C18) with particle size 5 micron with mobile phase containing TEA Buffer of pH 3.0 and Acetonitrile in proportion of 25:75 respectively. The flow rate was 1.0 ml/ min and effluent was monitored at 258 nm. The retention times were 2.39 min and 3.33 min respectively. The standard curve was linear over a working range of 05-35 μg/ml for both Amlodipine and Olmesartan and gave an average correlation coefficient of 0.999, and 0.999 for Amlodipine and Olmesartan respectively. The limit of quantitation (LOQ) of this method was 2μg/ml for Amlodipine and Olmesartan. The absolute recovery was 100% for Amlodipine and 100.3 for Olmesartan. Degradation products produced as a result of stress ...
Read the Medication Guide that comes with Escitalopram Oxalate Oral Solution before you start taking it and each time you get a refill. This Medication Guide does not take the place of talking to your healthcare provider about your medical condition or treatment.
Rapid and Sensitive RP-HPLC Method for Determination of Potential Genotoxic Impurity in Dasatinib Drug Substance, Sunil V Lanke, Sajid Syed Shahnawaz, Dhramveer Singh Shekhawat, Ni
Zirconia is known to be one of the best materials for the chromatographic support due to its excellent chemical, thermal and mechanical stability. In this work we report preparation and use of bovine...
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88637-37-0 - SPCKHVPPRJWQRZ-UHFFFAOYSA-N - Diphenhydramine citrate [USP] - Similar structures search, synonyms, formulas, resource links, and other chemical information.
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This application note describes how HTRF® and High throughput LabChip® ProteinEXact ensure a rapid and accurate characterization of bispecific antibodies (bsAbs).
The chemical and optical properties of colored dissolved organic matter (CDOM) have been the subject of numerous studies. However, this material has not been characterized to the point at which it can be identified by compound or compound class. The complex and homogenous nature of this material makes characterization studies challenging. CDOM absorbs ultraviolet and visible radiation and is highly photo reactive. The purpose of this study is to investigate the optical properties of fractions separated by reversed-phase high-performance liquid chromatography (HPLC) as well as the simple fractionation that occurs during the concentration and extraction of CDOM by C1s SEP-PAK. The focus of this study is the characterization of CDOM in the Suwannee and Manatee Rivers in western Florida. These rivers are a major source of CDOM to the Eastern Gulf of Mexico, which is a river-dominated environment. HPLC fractions were collected for the Suwannee River using reversed-phase HPLC and elution gradient. CDOM
Objective: To develop a validated stability-indicating analytical method for simultaneous estimation of Atenolol (ATN) and Hydrochlorothiazide (HTZ) in pharmaceutical combined dosage form by HPTLC. Method: A high performance thin layer chromatographic (HPTLC) method has been developed for the separation of ATN and HTZ on plates precoated with aluminium back silica gel 60 F254. Different mobile phases were used on trial and error basis for separation of two drugs. The final mobile phase selected for analysis was n-butanol: ethyl acetate: methanol: tetrahydrofuran in the ratio (1:2:2:1 v/v). Both the drugs showed maximum absorbance at 250 nm which was selected as the detection wavelength throughout the experimental work. Developed method was validated as per ICH guidelines. Forced degradation of drugs was carried out under various stress conditions and HPTLC method was used for analysing the stability of drugs. Result: HPTLC method was successfully developed for separation of ATN and HTZ with good ...
Fortschr Med. 1987 Aug 30;105(25):63. German.. Determination of (E)-1,2,3,4-tetrahydro-1,1,4,4-tetramethyl-6-(1-methyl-2- phenylethenyl) naphthalene, an antiacne agent, and its phenolic metabolite in plasma by reversed-phase high-performance liquid chromatography ...
HPLC Application #18828: Atenolol and Related Substances CRS using Kinetex 2.6um C18 100 x 4.6mm at 01.3ml/min. Column used: Kinetex® 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea Part#: 00D-4462-E0
The amino acid L-arginine together with its metabolites and related substances is in the center of many biologically important pathways, especially the urea cycle and the nitric oxide (NO) synthesis. Therefore, the concentrations of these substances in various biological fluids are of great interest …
Free Essays from Cram | Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the...
The test the FDA uses to certify each batch of these dyes before they legally may be used in drugs and cosmetics marketed in the United States no longer will be done by visible spectrophotometry, but by reversed-phase high performance liquid chromatography ...
DEVELOPMENT AND VALIDATION OF NOVEL SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF PIOGLITAZONE AND METFORMIN IN BULK AND FIXED DOSAGE FORMS BY AREA UNDER CURVE AND DUAL WAVELENGTH MODE
The present work describes a simple first derivative spectrophotometric method for simultaneous estimation of nebivolol and s-amlodipine in tablets. T..
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Disclosed is a single phase liquid composition comprising 0.5 to 30 wt % of a benzotrifluoride solvent, about 0.5 to about 50 wt % of a glycol ether cosolvent, up to about 50 wt % of an alkanol, up to about 10 wt % of a surfactant, and the remainder water. The composition is effective in cleaning hard surfaces, stripping paint, and as a carrier.
This paper outlines the development of a liquid chromatography method for simultaneous determination of approximately 20 amino acids in simple and complex mixtures that complies with the system suitability requirements of the Ph. Eur. general chapter (2.2.56).
Selenium (Se) and sulfur (S) speciation analysis in edible and medicinal Se enriched P. pulmonarius extracts was performed. Mycelium, colonized substrate, and fruiting bodies at different harvesting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS detector. Extraction efficiencies in enzymatically digested and aqueous extracts were between 45.3 and 109% fo ...
Please click on below links to download the course file: 1-What is HPLC? 2-Basic terms and concepts. 3-HPLC instrumentation and trends. 4-Introduction to chromatography software. 5-Types of HPLC; isocratic & gradient. 6-Qualitative and quantitative determination. 7-Normal & reverse phase chromatography modes. 8-HPLC column and trends. 9-Column selection in reverse phase chromatography. 10-HPLC method development. 11-Regulatory[…]. ...
SiliaBond Cyano (Si-CN) is a very versatile sorbent due to its polarity marking the separation between the polar and non-polar phases. This sorbent is particularly useful to reduce retention times and improve peak shapes when dealing with extremes in the polarity scale for both normal and reversed-phase chromatography.
|p|Silia|em||span style=color: #f37021;|Bond|/span||/em||sup|®|/sup| C18 (or C18) is the traditional matrix for reversed-phase chromatography. The high loading provides the highest degree of hydrophobicity commercially available. Considered as the l
Pill with imprint APO ESC 20 is White, Round and has been identified as Escitalopram Oxalate 20 mg (base). It is supplied by Apotex Corp..
There are different types of chromatography methods you can use for column chromatography. Here is a description of the most common types.
This paper presents the review of the methods used in research of the biological active substances hydrophobicity, a very important property. The biological activity of some substances depends on their pharmacokinetics and pharmacodynamics. These processes depend on the molecules capability to interact with two different media: aqueous (cells interior) and non-aqueous (cells membrane), or on the molecule lipophilicity. Today, great attention is given to investigation and systematic determination of drugs lipophilicity. In these researches chromatography methods have an important role ...
Dear Everyone: I am trying to transfer for western blot after Tris-Tricine-buffered electrophoresis which looks good for low molecular protein. But, it looks very difficult to transfer onto PVDF membrane with 1200mA.hr. There is no critical difference except containing glycerol(I think is for good resolution). Anyway, although I tried it under the condition, parts of high molecular bands are remained on gel. What should I do for complete transfer? I would appreciated if anyone who know trouble shooting make me know. Thank you. Sincerely yours, SK Park ...
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Gourmet Passport, Dineouts premium dining loyalty program has launched a one-of-a-kind health campaign called Eat Good Resolution 2020. The campaign is set to encourage... ...