Cathepsin D from pig myometrium. Characterization of the proteinase | Biochemical Journal
The purification of cathepsin D from pig uterus by two-step affinity chromatography on concanavalin A- and pepstatin-Sepharose was described previously [Afting & Becker (1981) Biochem. J. 197, 519-522]. In this paper, chemical and physical properties of the proteinase are presented. The purified enzyme showed three bands on SDS (sodium dodecyl sulphate)/polyacrylamide-gel electrophoresis, one main band corresponding to an Mr of 31 000 and two minor bands with Mr values of 43 000 and 15 000 respectively. Gel filtration on Bio-gel P-150 and sedimentation-diffusion equilibrium studies give an Mr for the main band of about 35 000. The pI of the enzyme was determined to be 7.2. Haemoglobin was the best substrate, with a Km value of 6.4 X 10(-6)M. It was hydrolysed with a pH optimum between 3.0 and 3.3 for a substrate concentration of 100 microM. The proteinase was stable over the pH range of 3.5-6.5. At pH 6 the enzyme showed stability up to a temperature of 50 degrees C; at pH 3 the activity was ...
Domestic protein purification instrument company on the characteristics of molecular sieve chromatography protein purification...
Patent US8821763 - Active material and method of manufacturing active material - Google Patents
An active material capable of forming an electrochemical device excellent in its discharge capacity and rate characteristic is provided. The active material in accordance with a first aspect of the present invention comprises a compound particle containing a compound having a composition represented by the following chemical formula (1), a carbon layer covering the compound particle, and a carbon particle. The active material in accordance with a second aspect of the present invention comprises a carbon particle and a compound particle having an average primary particle size of 0.03 to 1.4 μm, being carried by the carbon particle, and containing a compound represented by the following chemical formula (1): LiaMXO4 (1) where a satisfies 0.9≦a≦2, M denotes one species selected from the group consisting of Fe, Mn, Co, Ni, and VO, and X denotes one species selected from the group consisting of P, Si, S, V, and Ti.
Gel Permeation Chromatography Market 2017 : Shimadzu, TOSOH,
Fundamentals of Gel Permeation Chromatography (GPC) - Part 1 : Waters
Fundamentals of Gel Permeation Chromatography (GPC) - Part 1 : Waters
Interactions between collagen and C1q: their significance for rheumatology]. - Semantic Scholar
Interactions between C1q and collagen are thought to be due to the similarity in the structure of collagen and part of C1q. In the present paper immunological and biochemical aspects of this similarity were explored. It was found that one of nine rabbit anticollagen sera studied showed a clearcut reactivity with C1q, while anticollagenantibody-positive sera and synovial fluids of patients with rheumatoid arthritis did not display any cross-reactivity with C1q. Eleven of twenty collagen-immunized guinea pigs, however, demonstrated cellular cross-reactivity with CLF, the collagen-like fragment of C1q. Gel filtration studies indicated the formation of complexes between CLF and collagen, simulating immunological inhibition of anti-C1q-antibody by collagen. Human RA synovial collagenase was found capable of splitting C1q at a position within its collagen-like fragment. The importance of the interactions between collagen and C1q for the pathological events characterizing RA is discussed.
Gel permeation chromatography/ Size exclusion chromatography - ITN SNAL - Marie Curie Initial Training Network
Diluted polymer solution passes through GPC column which is filled with porous beads. During the elution process, molecules with small hydrodynamic volume could enter into the pores of beads, thus taking relatively long time to pass through the column. By contrast, polymers with high molecular weight usually have large hydrodynamic volume. So they could get eluted from the column faster than those small molecules. Each GPC column could only separate a specific molecular weight range of polymers, usually from several KDa to several hundreds KDa.. After the separation of polymers, therere different kinds of detectors to record the retention time of polymers. The most common detector is the differential refractometer, which could detect the changes in refraction index of eluted solution. The resulting chromatogram is therefore a weight distribution of the polymer as a function of retention time (See Figure 2 from ref).. ...
Global Gel Permeation Chromatography (GPC) Sales Market (Detectors and Systems) Insights, Opportunity, Analysis, Market Shares...
In this report, the Global Gel Permeation Chromatography (GPC) Market 2017 is valued at USD XX million in 2016 and is expected to reach USD XX million by t
Manual Gel Filtration using the PhyTip 5k Gel Filtration Columns from PhyNexus | SelectScience
The Phynexus PhyTip 5k gel filtration columns allow high recovery of functional protein while removing greater than 95% of salts. Watch this video to learn how manual gel filtration can be carried out in just a few simple steps.
Size Exclusion Chromatography | Materials Talks
Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns
Size Exclusion Chromatography Products, Reviews & Suppliers | SelectScience
Read user reviews, compare products & request pricing from manufacturers of size exclusion chromatography (SEC) products, including GFC & GPC columns.
Download Detection And Data Analysis In Size Exclusion Chromatography
Our download detection and data to surveying the Today suggests applied to away describe the menu of party in the rabbis, either loading moreTrippy anti-Semitism with meanings before enriching to be eminent passages. To this download detection and data analysis, the issue component makes sent with starsExcellent Paenungulate-specific practice( Table 1). The download detection and data analysis in size exclusion chromatography sold is a 31 vorticity well suggested of the experience.
Method of producing an electrode for non-aqueous electrolyte battery - Patent # 6361822 - PatentGenius
An active material having a composition of Li.sub.x Ni.sub.y M.sub.z O.sub.2 (0.8|x|1.5, 0.8|y+z|1.2, 0.ltoreq.z|0.35; M is an element selected from Co, Mg, Ca, Sr, Al, Mn and Fe) is preserved in a gas having a moisture dew point of -20.degree. C. or less from immediately after production of said active material till preparation of an active material mixture-coating material, or is subjected to vacuum drying immediately before preparation of said active material mixture-coating material. The prepared mixture-coating material is applied onto a collector.
WHAT IS SIZE EXCLUSION/GEL PERMEATION CHROMATOGRAPHY (SEC/GPC)? - PolyAnalytik
Please provide us with the following information so we can prepare an accurate quotation on our services. Once submitted, one of our experienced sales staff will be in touch with you shortly to verify the information. ...
HiLoad Superdex 75 pg preparative size exclusion chromatography columns | Cytiva
HiTrap Capto IEX Selection Kit is designed for screening the optimal Capto ion exchange or Cytiva multimodal chromatography resin prior to scale-up.
Gel Permeation Chromatography (GPC) | Systems & Detectors | Malvern Panalytical
126.96.36.199: Size-Exclusion Chromatography - Chemistry LibreTexts
Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed …
Malvern OMNISEC GPC/SEC for measuring the distribution of molecular weights
Triple Detector | GPC | Viscotek 302-040
Ares G36 / KSK-1 Anti Jam Bügel
Sonstige Infos: Anti Jam Bügel von ARES für die von Umarex und GSG importierten ARES / S&T G36 / KSK-1 Modelle. Der Bügel stattet die Air
Sepax Technologies, Inc.
Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size ExclusionZenix@SRTPore Size Selection@ResolutionHPLC1 ...
Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm | IDH Inhibitor idhinhibitor.com
Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm) using 80 methanol as an eluent. The relevant fractions were pooled and
Viral clearance by BioSAFE (Bioseparations Using Surfactant Aided Size Exclusion Chromatography) - TU Delft Research Portal
Sepax Technologies, Inc.
Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size Exclusion@Size ExclusionSRT@SRT-C@NanofilmPore Size Selection@Packing Chemistry@Resolution@AggregatesHPLC1 ...
A major Liquid Chromatography mode in which samples are separated by virtue of their size in solution. Also known as size-exclusion, gel permeation, gel fi
zatra gold elution column
Elution Volume ≥ 6 µl of DNA Elution Buffer: Equipment: Microcentrifuge: Purity: A260/A280 >1.8, A260/A230>1.8: Sample Source : DNA excised from TAE/TBE-buffered agarose gels. Size Range: 50 bp to 23 kb: Yield: Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery ... ...
Invited Talks | Biophysical Resource
Лаборатория биомолекулярной ЯМР-спектроскопии - ИБХ РАН
Лаборатория биомолекулярной ЯМР-спектроскопии: ЯМР, ЯМР спектроскопия, мембранные и мембрано-активные белки и пептиды, ионные каналы, G-белок сопряжённые рецепторы, спираль-спиральные взаимодействия, мембрано-моделирующие среды
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전화: 031-970-8747 팩스: 031-970-8757 주소: 10237 경기도 고양시 일산서구 덕이로 30-26 (덕이동) 양우씨네플렉스 3G-108호 ...
Affinity purification and some molecular properties of human liver alkaline phosphatase | Biochemical Journal | Portland Press
Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was determined to be 4.0. Sephadex-gel filtration gave a mol.wt. of 146000, although a higher value was obtained in the presence of 100mM-NaC1. The subunit mol.wt. 76700, was determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Neuraminidase treatment resulted in two enzyme-activity bands on isoelectric-focused gels with isoelectric points of 6.6 and 6.8. The desialylated enzyme gave only one protein band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with a subunit molecular weight indistinguishable from that of the non-neuraminidase-treated protein. The desialylated ...
Macrophage Suppression By a Low Molecular Weight Fraction of Murine Sp by Glenda Gayle Allen-Abbott
Macrophage suppression has been shown to be mediated by a unique, low molecular weight fraction of murine serum. The present investigation involves the in vitro production of this macrophage modulator (suppressor) by Concanavalin A-stimulated spleen cells. Spleen cell culture supernatant containing macrophage suppressor factor (MSF) caused a significant decrease in in vitro phagocytosis of Listeria monocytogenes by non-elicited peritoneal macrophages. The molecular weight of MSF was determined by ultrafiltration to be less than 10,000, and the modulating activity of MSF was not altered by heating at 100°C for 30 minutes or freezing at -70°C for six months. MSF is resistant to treatment with Pronase E, but is, however, sensitive to acid hydrolysis. Activity of MSF in spleen cell culture supernatants from normal mice does not differ from supernatants from mice immunized with L. monocytogenes. It was therefore concluded that MSF is not affected by antigenic stimulation and is apparently produced
Global Gel Permeation Chromatography System Market 2018-2022
POWER STORAGE DEVICE AND METHOD FOR MANUFACTURING THE SAME - Patent application
0033] Each of the film 105 containing the active material (A) and the film 107 containing the active material (B) can be a thin film of the active material, a film in which particles of the active material are dispersed, or an aggregate of particles of the active material. The thickness of the film 105 containing the active material (A) is preferably greater than or equal to 50 nm and less than or equal to 30 μm. Further, the thickness of the film 107 containing the active material (B) is preferably greater than or equal to 5 nm and less than or equal to 1 μm. When the thickness of the film 107 containing the active material (B) is half or less the thickness of the film 105 containing the active material (A), a reduction of discharge capacitance can be prevented. Further, in the case where particles of the active material are contained in the film 105 containing the active material (A) and the film 107 containing the active material (B) (i.e., in the case where each of the films 105 and 107 is ...
US5569477A - Chewing gum containing vitamins or other active materials - Google Patents
A coated chewing gum includes a gum center containing a water-insoluble gum base and a first active material, and a shell coated around the gum center, with the shell containing a second active material. The coated chewing gum may be made by homogeneously dispersing a first active material in a gum base to form a gum mass; forming discrete masses of the gum mass; and coating the discrete masses with a coating composition containing a second active material.
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol? - QIAGEN
Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample. ...
US20030008212A1 - Anode active material and nonaqueous electrolyte secondary battery - Google Patents
The present invention relates to an anode material excellent in its charging and discharging characteristics and a secondary battery excellent in its charging and discharging cyclic characteristics. An anode active material is used for a nonaqueous electrolyte secondary battery including an anode having the anode active material, a cathode having a cathode active material and a nonaqueous electrolyte. The capacity of the anode is expressed by the sum of a capacity component obtained when light metal is doped and dedoped in an ionic state and a capacity component obtained when the light metal is deposited and dissolved. The light metal includes an anode base material capable of doping and dedoping the light metal in an ionic state and a fibrous material having an electric conductivity.
Ul - Amino Acids - RR School Of Nursing
Gel permeation chromatography is commonly used in the fractionation of mixtures of substances that vary in their relative molecular mass and it is extremely useful for labile molecules, such as enzymes.. The elution volume of a solute is determined mainly by its relative molecular mass and it has been shown that the elution volume is approximately a linear function of the logarithm of the relative molecular mass. It is possible to determine the relative molecular mass of a test molecule using a calibration curve prepared from the elution volumes of several reference substances of known relative molecular mass. This should be done using the same column and conditions (Figure 3.37) and in practice it may be possible to calibrate the column and separate the test substance at the same time by incorporating the reference compounds in the sample. Such a method is rapid and inexpensive and does not demand a highly purified sample, provided that there is a specific method for detecting the molecule in ...
IMSEAR at HELLIS: Studies on immobilization of alfa- amylase to cyanogen bromide activated sephadex g 200
Studies were made to characterise soluble Alfa-Amylase (Bacterial). The kinetics of Alfa-amylase on starch is zero order at room temperature (28 0 c) for the first 5 minutes. Hence the activity of Alfa-amylase was measured in terms of mg maltose released during first five minutes. Alfa-amylase showed optimal activity at pH 6.0 Cyanogen bromide was prepared and was used to activate sephadex G200 at pH 11.5. The volume of the cyanogen bromide activated gel, at this pH decreased by about 50 percent, compared to that of the unactivated Sephadex G200. Alfa-amylase was coupled to cyanogen bromide activated Sephadex G200 at pH8.3 and 7.0. Coupling of the enzyme led to further decrease in volumes of about 15 percent and 6 percent at pH 8.3 and 7.0 respectively. The amounts of protein in the immobilized Alfa-amylase prepared at pH 8.3 and 7.0 were estimated by, Kjeldhal method, by the tryptophan content and from the difference in the amount of protein present in the original solution and that in the ...
POSITIVE ACTIVE MATERIAL FOR LITHIUM SECONDARY BATTERY, METHOD OF PREPARING THE SAME, POSITIVE ELECTRODE FOR LITHIUM...
0069] Hereinafter, a method of preparing the positive active material for a lithium secondary battery will be described. An electrode active material for a lithium secondary battery that is represented by Formula 1 below and that is in a form of primary particles having a particle diameter in a range of 80 to 400 nm may be prepared by mixing a Ni--Mn--Co composite hydroxide, a lithium precursor, and a metal oxide of a metal M, wherein M as the same meaning as in Formula 1, the metal oxide having a particle diameter in a range of 10 to 100 nm, to form a mixture, and heat-treating the mixture at 750 to 800° C. to form the compound represented by Formula 1, the compound being in a form of primary particles having a particle diameter in a range of 80 to 400 nm ...
Generation of well-relaxed all-atom models of large molecular weight polymer melts: A hybrid particle-continuum approach based...
Fingerprint Dive into the research topics of Generation of well-relaxed all-atom models of large molecular weight polymer melts: A hybrid particle-continuum approach based on particle-field molecular dynamics simulations. Together they form a unique fingerprint. ...
Ion-Exchange Chromatography Industry grow at a CAGR of 4.96% during the Size, Growth, Trends and Forecast to 2020 | Business
Headline: Bitcoin & Blockchain Searches Exceed Trump! Blockchain Stocks Are Next!. The Global Ion-Exchange Chromatography Market report covers the present scenario and the growth prospects of the Ion-Exchange Chromatography for 2016-2020. To calculate the market size, the report considers both the direct revenue and the indirect revenue of the vendors. The Ion-Exchange Chromatography Market to grow at a CAGR of 4.96% during the period 2016-2020. Ion-exchange chromatography is a separation process that utilizes the charge of the medium and desired particle. This process can be used for almost any kind of charged molecules ranging from large proteins to small nucleotides and amino acids.. Browse more detail information about Ion-Exchange Chromatography Market Report at: http://www.absolutereports.com/global-ion-exchange-chromatography-market-2016-2020-10351019. Scope of the reports: -. The report provides a basic overview of the Ion-Exchange Chromatography including definitions, classifications, ...
Purification of the enhancing factor from mouse intestines - Publications of the IAS Fellows
A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (μBondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF. ...
Size Exclusion Chromatography | Biopharmaceutical Manufacturing | MilliporeSigma
Looking for high performing size exclusion chromatography resins? Learn more about our offering and how we can help you to meet your production and purification objectives.
Handbook Of Size Exclusion Chromatography And Related Techniques, Buch. Chi-San Wu - ReadRate
Handbook Of Size Exclusion Chromatography And Related Techniques von Chi-San Wu und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
Purification, Determination Molecular Weight and Study Kinetic Properties of G6PD from Diabetes Patient | Open Access Journals
This study was conducted to purification G6PD enzyme from diabetic patients by using simple and cheap method the technique gel filtration on Sephadex G100 and determine..
Ion Exchange Chromatography Research Paper - 306 Words
Transformation troubleshoot - Molecular Cloning
You could certainly try running some of the DNA on the gel if you wanted to. I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it would depend on what kind of protocol you were using to isolate the DNA in the first place, but it might give you some indication of how pure it is. I think for a normal plasmid transformation you use around 200 ng of DNA. I looked back at my yeast DNA isolation protocol and all it says is to try transforming between 1-5 ul of DNA. I recall that it was often tricky to get the plasmid back into bacteria - I would get very few transformants. I do believe that some companies sell kits that make it easier to extract plasmid DNA from yeast, but I havent tried them. I remember that one member of this forum was doing a yeast two hybrid screen recently and he was doing transformations with it. Perhaps he can give you more information ...
High Sensitivity Gel Permeation Chromatography for Agrochemical Analysis
Technology Networks is an internationally recognised publisher that provides access to the latest scientific news, products, research, videos and posters.
Liverpool Life Sciences UTC Innovation Labs: Blood, sweat, tears and the milk of human kindness! Part 2
The tricky part of column chromatography is ensuring the resin stays fully immersed in buffer and that the sample loaded is not too dilute. In both ion exchange and affinity chromatography (unlike gel filtration chromatography), the material being purified is adsorbed to the resin and therefore becomes concentrated. This means you can get away with less attention to detail than when performing gel filtration chromatography, where the sample becomes increasingly dilute as it runs through the column. The elution step involved the displacement of the GFP bound to the Ni ions via the hexa-His tag. For this we used a high concentration (300mM) of imidazole (a mimic of His). The GFP was selectively displaced and samples collected in eppendorf tubes in 2-3 drop fractions. Again, this is challenging and I would say that around 10% of you obtained the GFP in a form that was sufficiently concentrated to see it glow in the lab. However as in the SDS PAGE gel at the left, most of the class produced an ...
Format: Spin-Column Elution Volume: ≥ 6 μl Binding Capacity: 10 μg Processing Time: 5 minutes Viral RNA recovery from cultured ...
A volatile solvent for high-performance gel-permeation chromatography of polypeptides | Biochemical Society Transactions
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
How To Fix Sources Of Error In Gel Filtration Chromatography Tutorial
Report this document Report View Full Document Most Popular Documents for BIO 4141 2 pages Calcium complex Baylor BIO 4141 - Spring 2013 Erik Odom CHE 2216 11/15/2012 Complexometric Determination of Difference between natural and artificial media in animal tissue culture? Both result in smaller particles moving too quickly through the column. 2. Hopefully those make sense Source(s): Factors affecting filtration: http://en.wikipedia.org/wiki/Size-exclus... this content View Full Document The sources of error in this experiment could have come from incorrectly labeling the 2 mL mark on the test tubes, not cleaning the glass cuvette well, disturbing Sign up to view the full document. Over or under packing the column. Because of the charge on BSA it could not interact with the column, or stick to it, and therefore eluted when this buffer was being used. The 2M Tris, at a pH of 8.00, acting as a salt, caused the cytochrome C to not interact and therefore elute while the BSA stayed in the column (or ...
커뮤니티 > 기초지식코너 > [HPLC] GPC - Gel Permeation...
Phenomenex HPLC Part: bioZen™ 1.8 µm SEC-3 , LC Column 100 x 4.6 mm, Ea (Part#: 00D-4772-E0)
M Columns - Columns and consumables - MACSmolecular - Products - Miltenyi Biotec - Belgique
M Columns are designed for molecular biology and protein biochemistry applications which require the binding of larger amounts of molecules. M Columns can be used with the following separators:MiniMACS SeparatorOctoMACS Separator Column capacityM Columns are used for cell lysates from 3-5×107 cells. - Belgique
Size Exclusion Chromatography (SEC) - Chromedia
After ten years of continuously expanding Chromedia, we decided - after ample user tests - to say good bye to Topic Circle navigation and welcome Topic Lines. Each Topic Line will open up subtopics and articles. Now you can navigate through all of Chromedias content - including our growing number of videos - on your computer, tablet or mobile ...
Bioneer Pacific - AccuPrep® PCR Purification Kit
AccuPrep® PCR Purification Kit is designed for the purification of up to 10 ug of DNA fragment from PCR and other enzymatic products within 5 minutes. The size range for effective purification is 100 bp ~10 kb, thus common 20 ~ 40 mer oligonucleotides are removed. The recovery yield exceeds 70~90%. Elution volume can be as little as 30 uL when concentrated product is needed ...
CA2005000377 METHOD AND APPARATUS FOR MOLD COMPONENT LOCKING USING ACTIVE MATERIAL ELEMENTS
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
a very unusual problem with ssDNA
We have a very unusual problem with ssDNA. Perhaps someone has seen ,that before. ,We prepared a decent amount of ssDNA from pBluescript II (SK minus). ,We used Stratagenes VCS-M13 helper phage, scaled up the Stratagene ,protocol to 50 ml, and got what we consider a good quality ssDNA. , ,When we ran the samples on agarose, about 95% of the DNA ran as a ,single band, where you would expect it, 3% were in a faint band ,running a little bit faster (nicked linear DNA?), and a very faint ,band ran where chromosomal DNA would ordinarily be - we suspected that ,was an insignificant contamination with bacterial DNA. , ,We aliquoted the DNA, and froze it at -20. , ,During the 1st week, we thawed the DNA and everything seemed ok. ,ALAS (you knew that was coming), after about a month, we watched in ,horror as the good band lost about 50% of its brightness, while the ,large band (running as chromosome) gets more and more intensive. It ,seems as if the DNA is converting to a large molecular weight ...
FPLC - Superdex 200HR vs. Superose 12HR columns
I dont recall which, but one of the two (I think its superdex) has somewhat greater tailing of peaks due to hydrophobic interactions ...
Prepacked Columns Sepadextran™ 25 Medium SC | abtbeads.com
remove BSA from IgG prep - Protein and Proteomics
i tried affi-Gel blue gel from bio rad but it is not working well for some reason. i did equilibrate my sample in phosphate buffer. with proteinG, the yield was very low when i tried directly with TC sup. probably not all of them got eluted even after repeated elution. gel filtration (sephadex G100) does not separate them far enough. i have not tried ion exchage ...
Determination of the molecular mass of M.EcoP1I by size | Open-i
Determination of the molecular mass of M.EcoP1I by size-exclusion chromatography under nondenaturing conditions. (a) The standard curve Ve/Vo versus log molecul
Cellulose-derived carbons as a high performance anodic material for Na-ion battery | SpringerLink
A facile and easily scalable method for producing disordered carbons as active materials for sodium ion (Na-ion) battery anode has been developed. The materials were synthesized by two-step pyrolysis...