The purification of cathepsin D from pig uterus by two-step affinity chromatography on concanavalin A- and pepstatin-Sepharose was described previously [Afting & Becker (1981) Biochem. J. 197, 519-522]. In this paper, chemical and physical properties of the proteinase are presented. The purified enzyme showed three bands on SDS (sodium dodecyl sulphate)/polyacrylamide-gel electrophoresis, one main band corresponding to an Mr of 31 000 and two minor bands with Mr values of 43 000 and 15 000 respectively. Gel filtration on Bio-gel P-150 and sedimentation-diffusion equilibrium studies give an Mr for the main band of about 35 000. The pI of the enzyme was determined to be 7.2. Haemoglobin was the best substrate, with a Km value of 6.4 X 10(-6)M. It was hydrolysed with a pH optimum between 3.0 and 3.3 for a substrate concentration of 100 microM. The proteinase was stable over the pH range of 3.5-6.5. At pH 6 the enzyme showed stability up to a temperature of 50 degrees C; at pH 3 the activity was ...
An active material capable of forming an electrochemical device excellent in its discharge capacity and rate characteristic is provided. The active material in accordance with a first aspect of the present invention comprises a compound particle containing a compound having a composition represented by the following chemical formula (1), a carbon layer covering the compound particle, and a carbon particle. The active material in accordance with a second aspect of the present invention comprises a carbon particle and a compound particle having an average primary particle size of 0.03 to 1.4 μm, being carried by the carbon particle, and containing a compound represented by the following chemical formula (1): LiaMXO4 (1) where a satisfies 0.9≦a≦2, M denotes one species selected from the group consisting of Fe, Mn, Co, Ni, and VO, and X denotes one species selected from the group consisting of P, Si, S, V, and Ti.
Press release - Gel Permeation Chromatography Market - Gel Permeation Chromatography Market 2017 : Shimadzu, TOSOH, Waters, Malvern, Agilent Technologies - published on openPR.com
This module covers the first part of the fundamentals of Waters Gel Permeation Chromatography (GPC). Topics included in this module: monomers and polymers, polymer analysis techniques and applications, size-exclusion chromatography, and detectors.
This module covers the first part of the fundamentals of Waters Gel Permeation Chromatography (GPC). Topics included in this module: monomers and polymers, polymer analysis techniques and applications, size-exclusion chromatography, and detectors.
Interactions between C1q and collagen are thought to be due to the similarity in the structure of collagen and part of C1q. In the present paper immunological and biochemical aspects of this similarity were explored. It was found that one of nine rabbit anticollagen sera studied showed a clearcut reactivity with C1q, while anticollagenantibody-positive sera and synovial fluids of patients with rheumatoid arthritis did not display any cross-reactivity with C1q. Eleven of twenty collagen-immunized guinea pigs, however, demonstrated cellular cross-reactivity with CLF, the collagen-like fragment of C1q. Gel filtration studies indicated the formation of complexes between CLF and collagen, simulating immunological inhibition of anti-C1q-antibody by collagen. Human RA synovial collagenase was found capable of splitting C1q at a position within its collagen-like fragment. The importance of the interactions between collagen and C1q for the pathological events characterizing RA is discussed.
In this report, the Global Gel Permeation Chromatography (GPC) Market 2017 is valued at USD XX million in 2016 and is expected to reach USD XX million by t
The Phynexus PhyTip 5k gel filtration columns allow high recovery of functional protein while removing greater than 95% of salts. Watch this video to learn how manual gel filtration can be carried out in just a few simple steps.
Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns
Read user reviews, compare products & request pricing from manufacturers of size exclusion chromatography (SEC) products, including GFC & GPC columns.
Our download detection and data to surveying the Today suggests applied to away describe the menu of party in the rabbis, either loading moreTrippy anti-Semitism with meanings before enriching to be eminent passages. To this download detection and data analysis, the issue component makes sent with starsExcellent Paenungulate-specific practice( Table 1). The download detection and data analysis in size exclusion chromatography sold is a 31 vorticity well suggested of the experience.
An active material having a composition of Li.sub.x Ni.sub.y M.sub.z O.sub.2 (0.8|x|1.5, 0.8|y+z|1.2, 0.ltoreq.z|0.35; M is an element selected from Co, Mg, Ca, Sr, Al, Mn and Fe) is preserved in a gas having a moisture dew point of -20.degree. C. or less from immediately after production of said active material till preparation of an active material mixture-coating material, or is subjected to vacuum drying immediately before preparation of said active material mixture-coating material. The prepared mixture-coating material is applied onto a collector.
The Malvern OMNISEC Gel Permeation Chromatography (GPC)/Size Exclusion Chromatography (SEC) uses multi-detection technology for characterising both proteins and polymers.
Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography, is a technique that employs columns to separate polymers and proteins according to their hydrodynamic volume in order to determine molecular size and weight.
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Ted to concentration and fractionation on a Sephadex LH-20 column (2.8 6 33 cm) using 80 methanol as an eluent. The relevant fractions were pooled and
Лаборатория биомолекулярной ЯМР-спектроскопии: ЯМР, ЯМР спектроскопия, мембранные и мембрано-активные белки и пептиды, ионные каналы, G-белок сопряжённые рецепторы, спираль-спиральные взаимодействия, мембрано-моделирующие среды
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Macrophage suppression has been shown to be mediated by a unique, low molecular weight fraction of murine serum. The present investigation involves the in vitro production of this macrophage modulator (suppressor) by Concanavalin A-stimulated spleen cells. Spleen cell culture supernatant containing macrophage suppressor factor (MSF) caused a significant decrease in in vitro phagocytosis of Listeria monocytogenes by non-elicited peritoneal macrophages. The molecular weight of MSF was determined by ultrafiltration to be less than 10,000, and the modulating activity of MSF was not altered by heating at 100°C for 30 minutes or freezing at -70°C for six months. MSF is resistant to treatment with Pronase E, but is, however, sensitive to acid hydrolysis. Activity of MSF in spleen cell culture supernatants from normal mice does not differ from supernatants from mice immunized with L. monocytogenes. It was therefore concluded that MSF is not affected by antigenic stimulation and is apparently produced
... About Gel Permeation Chromatography GPC is an analytical method used for the separation - Market research report and industry analysis - 11819766
0033] Each of the film 105 containing the active material (A) and the film 107 containing the active material (B) can be a thin film of the active material, a film in which particles of the active material are dispersed, or an aggregate of particles of the active material. The thickness of the film 105 containing the active material (A) is preferably greater than or equal to 50 nm and less than or equal to 30 μm. Further, the thickness of the film 107 containing the active material (B) is preferably greater than or equal to 5 nm and less than or equal to 1 μm. When the thickness of the film 107 containing the active material (B) is half or less the thickness of the film 105 containing the active material (A), a reduction of discharge capacitance can be prevented. Further, in the case where particles of the active material are contained in the film 105 containing the active material (A) and the film 107 containing the active material (B) (i.e., in the case where each of the films 105 and 107 is ...
A coated chewing gum includes a gum center containing a water-insoluble gum base and a first active material, and a shell coated around the gum center, with the shell containing a second active material. The coated chewing gum may be made by homogeneously dispersing a first active material in a gum base to form a gum mass; forming discrete masses of the gum mass; and coating the discrete masses with a coating composition containing a second active material.
Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample. ...
The present invention relates to an anode material excellent in its charging and discharging characteristics and a secondary battery excellent in its charging and discharging cyclic characteristics. An anode active material is used for a nonaqueous electrolyte secondary battery including an anode having the anode active material, a cathode having a cathode active material and a nonaqueous electrolyte. The capacity of the anode is expressed by the sum of a capacity component obtained when light metal is doped and dedoped in an ionic state and a capacity component obtained when the light metal is deposited and dissolved. The light metal includes an anode base material capable of doping and dedoping the light metal in an ionic state and a fibrous material having an electric conductivity.
Gel permeation chromatography is commonly used in the fractionation of mixtures of substances that vary in their relative molecular mass and it is extremely useful for labile molecules, such as enzymes.. The elution volume of a solute is determined mainly by its relative molecular mass and it has been shown that the elution volume is approximately a linear function of the logarithm of the relative molecular mass. It is possible to determine the relative molecular mass of a test molecule using a calibration curve prepared from the elution volumes of several reference substances of known relative molecular mass. This should be done using the same column and conditions (Figure 3.37) and in practice it may be possible to calibrate the column and separate the test substance at the same time by incorporating the reference compounds in the sample. Such a method is rapid and inexpensive and does not demand a highly purified sample, provided that there is a specific method for detecting the molecule in ...
Studies were made to characterise soluble Alfa-Amylase (Bacterial). The kinetics of Alfa-amylase on starch is zero order at room temperature (28 0 c) for the first 5 minutes. Hence the activity of Alfa-amylase was measured in terms of mg maltose released during first five minutes. Alfa-amylase showed optimal activity at pH 6.0 Cyanogen bromide was prepared and was used to activate sephadex G200 at pH 11.5. The volume of the cyanogen bromide activated gel, at this pH decreased by about 50 percent, compared to that of the unactivated Sephadex G200. Alfa-amylase was coupled to cyanogen bromide activated Sephadex G200 at pH8.3 and 7.0. Coupling of the enzyme led to further decrease in volumes of about 15 percent and 6 percent at pH 8.3 and 7.0 respectively. The amounts of protein in the immobilized Alfa-amylase prepared at pH 8.3 and 7.0 were estimated by, Kjeldhal method, by the tryptophan content and from the difference in the amount of protein present in the original solution and that in the ...
0069] Hereinafter, a method of preparing the positive active material for a lithium secondary battery will be described. An electrode active material for a lithium secondary battery that is represented by Formula 1 below and that is in a form of primary particles having a particle diameter in a range of 80 to 400 nm may be prepared by mixing a Ni--Mn--Co composite hydroxide, a lithium precursor, and a metal oxide of a metal M, wherein M as the same meaning as in Formula 1, the metal oxide having a particle diameter in a range of 10 to 100 nm, to form a mixture, and heat-treating the mixture at 750 to 800° C. to form the compound represented by Formula 1, the compound being in a form of primary particles having a particle diameter in a range of 80 to 400 nm ...
Headline: Bitcoin & Blockchain Searches Exceed Trump! Blockchain Stocks Are Next!. The Global Ion-Exchange Chromatography Market report covers the present scenario and the growth prospects of the Ion-Exchange Chromatography for 2016-2020. To calculate the market size, the report considers both the direct revenue and the indirect revenue of the vendors. The Ion-Exchange Chromatography Market to grow at a CAGR of 4.96% during the period 2016-2020. Ion-exchange chromatography is a separation process that utilizes the charge of the medium and desired particle. This process can be used for almost any kind of charged molecules ranging from large proteins to small nucleotides and amino acids.. Browse more detail information about Ion-Exchange Chromatography Market Report at: http://www.absolutereports.com/global-ion-exchange-chromatography-market-2016-2020-10351019. Scope of the reports: -. The report provides a basic overview of the Ion-Exchange Chromatography including definitions, classifications, ...
A unique polypeptide, called enhancing factor (EF), which enhances the binding of labeled epidermal growth factor (EGF) to cells, has been isolated. It has been purified to homogeneity from the acid-soluble proteins of mouse intestines. Earlier, EF was partially purified by two cycles of gel-permeation chromatography on Bio-Gel columns. We now report the final purification of EF on high-performance liquid chromatography (HPLC), using a reverse-phase column (μBondapak C18). The purity of the protein was confirmed when a single peak was obtained in HPLC. Also, a single protein band was obtained in SDS-PAGE. Purified EF has the same properties in vitro as those reported earlier for partially purified EF. ...
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This study was conducted to purification G6PD enzyme from diabetic patients by using simple and cheap method the technique gel filtration on Sephadex G100 and determine..
g A. Ion exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in
You could certainly try running some of the DNA on the gel if you wanted to. I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it would depend on what kind of protocol you were using to isolate the DNA in the first place, but it might give you some indication of how pure it is. I think for a normal plasmid transformation you use around 200 ng of DNA. I looked back at my yeast DNA isolation protocol and all it says is to try transforming between 1-5 ul of DNA. I recall that it was often tricky to get the plasmid back into bacteria - I would get very few transformants. I do believe that some companies sell kits that make it easier to extract plasmid DNA from yeast, but I havent tried them. I remember that one member of this forum was doing a yeast two hybrid screen recently and he was doing transformations with it. Perhaps he can give you more information ...
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The tricky part of column chromatography is ensuring the resin stays fully immersed in buffer and that the sample loaded is not too dilute. In both ion exchange and affinity chromatography (unlike gel filtration chromatography), the material being purified is adsorbed to the resin and therefore becomes concentrated. This means you can get away with less attention to detail than when performing gel filtration chromatography, where the sample becomes increasingly dilute as it runs through the column. The elution step involved the displacement of the GFP bound to the Ni ions via the hexa-His tag. For this we used a high concentration (300mM) of imidazole (a mimic of His). The GFP was selectively displaced and samples collected in eppendorf tubes in 2-3 drop fractions. Again, this is challenging and I would say that around 10% of you obtained the GFP in a form that was sufficiently concentrated to see it glow in the lab. However as in the SDS PAGE gel at the left, most of the class produced an ...
Format: Spin-Column Elution Volume: ≥ 6 μl Binding Capacity: 10 μg Processing Time: 5 minutes Viral RNA recovery from cultured ...
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Report this document Report View Full Document Most Popular Documents for BIO 4141 2 pages Calcium complex Baylor BIO 4141 - Spring 2013 Erik Odom CHE 2216 11/15/2012 Complexometric Determination of Difference between natural and artificial media in animal tissue culture? Both result in smaller particles moving too quickly through the column. 2. Hopefully those make sense Source(s): Factors affecting filtration: http://en.wikipedia.org/wiki/Size-exclus... this content View Full Document The sources of error in this experiment could have come from incorrectly labeling the 2 mL mark on the test tubes, not cleaning the glass cuvette well, disturbing Sign up to view the full document. Over or under packing the column. Because of the charge on BSA it could not interact with the column, or stick to it, and therefore eluted when this buffer was being used. The 2M Tris, at a pH of 8.00, acting as a salt, caused the cytochrome C to not interact and therefore elute while the BSA stayed in the column (or ...
HPLC Part: 00D-4772-E0 bioZen™ 1.8 µm SEC-3 , LC Column 100 x 4.6 mm, Ea Separation Mode: Gel Filtration Chromatography, Gel Filtration Chromatography (GFC) Format: Column
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AccuPrep® PCR Purification Kit is designed for the purification of up to 10 ug of DNA fragment from PCR and other enzymatic products within 5 minutes. The size range for effective purification is 100 bp ~10 kb, thus common 20 ~ 40 mer oligonucleotides are removed. The recovery yield exceeds 70~90%. Elution volume can be as little as 30 uL when concentrated product is needed ...
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We have a very unusual problem with ssDNA. Perhaps someone has seen ,that before. ,We prepared a decent amount of ssDNA from pBluescript II (SK minus). ,We used Stratagenes VCS-M13 helper phage, scaled up the Stratagene ,protocol to 50 ml, and got what we consider a good quality ssDNA. , ,When we ran the samples on agarose, about 95% of the DNA ran as a ,single band, where you would expect it, 3% were in a faint band ,running a little bit faster (nicked linear DNA?), and a very faint ,band ran where chromosomal DNA would ordinarily be - we suspected that ,was an insignificant contamination with bacterial DNA. , ,We aliquoted the DNA, and froze it at -20. , ,During the 1st week, we thawed the DNA and everything seemed ok. ,ALAS (you knew that was coming), after about a month, we watched in ,horror as the good band lost about 50% of its brightness, while the ,large band (running as chromosome) gets more and more intensive. It ,seems as if the DNA is converting to a large molecular weight ...
I dont recall which, but one of the two (I think its superdex) has somewhat greater tailing of peaks due to hydrophobic interactions ...
Prepacked Columns Sepadextran™ 25 Medium SC are hydrated gel filtration columns designed for rapid and efficient removal of small molecules (salts, dyes, ammonia …) from proteins, enzymes or antibodies samples.|br /||br /| Prepacked columns contain Sep
i tried affi-Gel blue gel from bio rad but it is not working well for some reason. i did equilibrate my sample in phosphate buffer. with proteinG, the yield was very low when i tried directly with TC sup. probably not all of them got eluted even after repeated elution. gel filtration (sephadex G100) does not separate them far enough. i have not tried ion exchage ...
Determination of the molecular mass of M.EcoP1I by size-exclusion chromatography under nondenaturing conditions. (a) The standard curve Ve/Vo versus log molecul