Complete information for PSAP gene (Protein Coding), Prosaposin, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Expression and Purification of recombinant Green Fluorescent Protein ABSTRACT: The purpose of this experiment was to determine if a His-6 tagged recombinant form of Green Fluorescent Protein could be expressed in a pRSETA vector of E. Coli. This was determined through multiple procedures beginning with purifying the sample with Ni +2 agarose chromatography which showcased the relative fluorescent activity of the samples, which elution sample two (E2) had approximately 100,592.2 RFU/mg . The yield of total protein was found by use of a Bradford Assay and a standard curve. The purity of the GFP was determined by comparing the intensity of bands that appeared at around 31.4 kDa (the molecular weight of rGFP) to a molecular weight ladder on an SDS-PAGE gel. The Western Blot test, utilizing a nitrocellulous membrane, confirmed the expression of rGFP. The Western Blot confirmed that the correct bands were analyzed in the SDS-PAGE gel which E3 had an estimated purity of 0.4, indicating a yield of ...
Recombinant Human TEC, catalytic domain [359-631 amino acids] was expressed as N-terminal GST-fusion protein (59 kDa) usingbaculovirusexpression system. GST-TEC was purified by using glutathione sepharose chromatography and anion exchange chromatography.
A.T. Hanke, M.E. Klijn, P.D.E.M. Verhaert, L.A.M. van der Wielen, M. Ottens, M.H.M. Eppink, E.J.A.X. van de Sandt, Prediction of protein retention times in hydrophobic interaction chromatography by robust statistical characterization of their atomic-level surface properties, Biotechnol Prog. (2015) 1520-6033, http://dx.doi.org/10.1002/btpr.2219 ...
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|i|Mycobacterium avium|/i| subsp. |i|paratuberculosis|/i| (Map) contains PPE family antigens which are Proline and glutamic acid rich and may play important role as T cell antigens. Hence the identification and generation of antigens are necessary for immunological characterization. In the present study, the epitopic region of a unique PPE gene encoding 34.9 kDa protein from Map was amplified by polymerase chain reaction. The gene was cloned into |i|Escherichia coli|/i| vector pQE30 UA. The recombinant plasmid designated as pQPPE was transformed into |i|E. coli|/i| M15 and induced with IPTG revealed the high level expression of 37.1 kDa His-fusion protein (34.9 kDa PPE and 2.2 kDa His-tag), which was confirmed by immunoblotting. Recombinant PPE protein was then purified by Ni-NTA agarose chromatography. The polyclonal antiserum raised against purified recombinant PPE protein reacted with expressed 37.1 kDa His-fusion protein as well as with Map sonicate. The protein elicited
Hydrophobic Interaction Chromatography is a separation technique that uses the properties of hydrophobicity to separate proteins from one another. In this type of chromatography, hydrophobic groups such as phenyl, octyl, or butyl, are attached to the stationary column. Proteins that pass through the column that have hydrophobic amino acid side chains on their surfaces are able to interact with and bind to the hydrophobic groups on the column. HIC separations are often designed using the opposite conditions of those used in ion exchange chromatography. In this separation, a buffer with a high ionic strength, usually ammonium sulfate, is initially applied to the column. The salt in the buffer reduces the solvation of sample solutes thus as solvation decreases, hydrophobic regions that become exposed are adsorbed by the medium. ...
The design of gradient simulated moving bed (SMB) chromatographic processes requires an appropriate selection of the chromatographic system followed by the determination of adsorption isotherm parameters in the relevant range of mobile phase conditions. The determination of these parameters can be q …
Agarose偶联6X His tag®抗体(ab1231)经IP实验严格验证,实验条件参看说明书。Abcam对所有产品均提供质保服务和专属技术支持,中国75%以上现货。
Its only fair to share… Sacubitril, AHU 377 NEPRILYSIN INHIBITOR FOR HEART FAILURE CAS 149709-62-6 CAS SODIUM SALT 149690-05-1 (2R,4S)-5-(biphenyl-4-yl)-4-((3-carboxypropionyl)amino)-2-methylpentanoic acid ethyl ester 5-(Biphenyl-4-yl)-4(S)-(3-carboxypropionamido)-2(R)-methylbutyric acid ethyl ester N-(3-carboxy-1-oxopropyl)-(4S)-(p-phenylphenylmethyl)-4-amino-2R-methyl butanoic acid ethyl ester [1,1′-Biphenyl]-4-pentanoic acid, γ-[(3-carboxy-1-oxopropyl)amino]-α-methyl-, α-ethyl ester, (αR,γS)- [1,1′-Biphenyl]-4-pentanoic acid, γ-[(3-carboxy-1-oxopropyl)amino]-α-methyl-, ethyl ester, [S-(R*,S*)]- (2R,4S)-4-[(3-Carboxy-1-oxopropyl)amino]-4-[(p-phenylphenyl)methyl]-2-methylbutanoic acid ethyl ester (2R,4S)-5-(Biphenyl-4-yl)-4-[(3-carboxypropionyl)amino]-2-methylpentanoic acid ethyl ester Formula C24H29NO5 MW 411.49 …. Read more ...
This was a randomized, double-blind, placebo-controlled, dose escalation study to assess the safety and tolerability of 100 mg and 200 mg of inhaled Alpha-1 HC administered once a day for three weeks in subjects aged 18 years and older with cystic fibrosis (CF). The treatment duration in this study was intended to provide multi-dose safety information prior to proceeding to longer durations of exposure ...
This webcast presents a series experiments in which in-process mAb material was spiked with MVM-MVP and processed through Anion Exchange and Cation Exchange, and a a viral clearance study was performed on Hydrophobic Interaction Chromatography (HIC) resins
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In 1973, Roosevelt Cole, on his own behalf and on behalf of all present and future black inmates in the Panola County jail, sued the individual members of the Board of Supervisors of Panola County, Mississippi and the Sheriff for neglecting to look into the state of the prisons as required by Mississippi statute. The plaintiff complained of the unsanitary and unsafe conditions, the absence of fair procedures, physical abuse, denial of religious and political freedoms, inadequate medical attention, denial of a right to fair trial, lack of educational, recreational, and rehabilitation facilities, denial of essential communication due to poverty, inadequate diet, and racial discrimination against black inmates ...
Author: Ta, H. D. et al.; Genre: Poster; Title: Analysis of the methyl formate hydrolysis kinetics taking volume changes into account
Explore our array of standards for IEX, SEC (gel filtration), hydrophobic interaction chromatography or qualitative organic acid/carbohydrate analysis.
DUBLIN, Dec. 7, 2017 /PRNewswire/ --The Zeolite Molecular Sieve Market: Global Industry Analysis, Trends, Market Size and Forecasts up to 2023 report has ...
Sep 25 2019 · Whether working with soils or rocks the void ratio and porosity difference is important to understand. The void ratio (e) is the ratio of the volume of voids (V v) to the volume of solids (V s).Porosity (n) on the other hand is the ratio of the volume of voids (V v) to the total volume (V) or volume of the voids plus the volume of the solids (V v V s).. Service Online ...
Windows 8 currently still in its pre-beta phase of development, has an official build number for 8176. Microsoft is slated to release the Beta of its next version of operating system sometime in late February. Today, a
Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.
The present invention relates to functionalized molecular sieves that are useful as shape-selective adsorbents and catalysts. The inventive molecular sieves have a crystalline framework and include micropores of substantially uniform size and shape formed therein. These micropores contain one or more accessible organic moieties that are linked to the crystalline framework by a carbon-silicon ond. Shape selectivity of the inventive molecular sieves may be modulated by varying the size and shape of the micropores (which is a function of the particular molecular sieve being synthesized) and the organic moiety incoporated therein. In preferred embodiments, the molecular sieves of the present invention include crosslinked monomers of the formula wherein: X is a subsituted or unsubstituted moiety and R1 and R2 are each independently either oxygen, or a substituted or unsubstituted moiey, wherein the moiety is selected from the group consisting of C1-C20 alkyl, C2-C10 alkenyl, C2-C20 alkynyl, cycloalkyl,
The immunogen is Aflatoxin B1 (AFB1)-BSA conjugates. The antibody was affinity purified with an AFB1-Agarose column and competitively eluted by free AFB1. The antibody was conjugated to peroxidase (HRP) by reductive amination. This anti-AFB1 HRP conjugates could be utilized for detection and quantization of the food-borne mycotoxin AFB1. ...
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Glycoprotein Ib-IX-V (GPIb-IX-V) is a platelet adhesion receptor complex that initiates platelet aggregation. Glycoprotein Iba (GPIba) is the central component of the GPIb-IX-V complex, anchoring the complex to the cytoskeleton and harboring the binding site for von Willebrand factor (vWF). Previous studies suggest that the coagulation function in pigs differs from that in humans, especially with respect to the interaction between vWF and platelets. However, we have little knowledge about the function of porcine platelets, which is important with regard to studies of cardiovascular disease, clotting, and surgery that use pigs as animal models. To extend this information, we cloned and analyzed the porcine GPIba sequence. Porcine GPIba contains 1891 nucleotides and includes an open reading frame that encodes 627 amino acids. The nucleotide sequence showed 67% identity with human GPIba, whereas the deduced amino acid sequences were 59% identical. The vWF binding domain shares the highest identity ...
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GE Healthcare Sepharose Agarose Gel Filtration Media Sepharose CL-2B gel; 1000mL GE Healthcare Sepharose Agarose Gel Filtration Media Gel Filtration Media...
A method of preparing high purity procoagulant protein comprising the steps of (a) adsorbing a VIII:C/VIII:RP complex from a plasma or commercial concentrate source of factor VIII onto agarose beads bound to a monoclonal antibody specific to VIII:RP, (b) eluting VIII:C with a salt solution, (c) adsorbing the eluted VIII:C on an animohexyl agarose column and eluting the VIII:C with a salt solution.
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UltraPure™ Agarose,UltraPure™ Agarose resolves DNA and RNA fragments from 100 bp to >30 kb. UltraPure™ Agarose is a standard melting temperature multi-purpose agarose that is ideal for routine separation analysis. Its high gel strength specification ensures that gels will not break during handling nor d,medicine,medical supply,medical supplies,medical product
Besta™ LE Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high resolution sharp DNA bands with high clarity and low background. Its optimized gel st
Agarose偶联VSV-G tag抗体(ab3843)经WB, ELISA, ICC实验严格验证,被1篇文献引用,实验条件参看说明书。Abcam对所有产品均提供质保服务和专属技术支持,中国75%以上现货。