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Purpose: To evaluate the potential of a bioactive coating based on chondroitin sulfate (CS) and tethered epidermal growth factor (EGF) for the improvement of healing around stent-grafts (SG). Methods: The impact of the bioactive coating on cell survival was tested in vitro on human vascular cells using polyethylene terephtalate films (PET) as a substrate. After being transferred onto a more "realistic" material (expanded poly(tetrafluoroethylene), ePTFE), the durability and mechanical behavior of the coating in addition to cell survival were studied. Preliminary in vivo testing was performed in a canine iliac aneurysm model reproducing type I endoleaks (3 animals with 1 control and 1 bioactive SG for each). Results: The CS and EGF coatings significantly increased survival of human smooth muscle cells and fibroblasts, compared with bare PET or ePTFE (P,.05). The coating also displayed good durability over 30 days according to ELISA and cell-survival tests. The coating did not affect the ...
I thought these nutraceuticals were a bunch of boloney until, with no other management changes, my friends gelding went from lame at a walk to sound at a trot - he had been lame for some time with arthritis. (he is on chondroitin sulfate) What else is out there worth trying, and AERC legal? Maybe something to keep my muscles from getting so sore/stiff after a ride. Shelly in DE ...
Small ubiquitin like modifier (SUMO) proteins are known to regulate many important cellular processes such as transcription and apoptosis. Recently, hybrid SUMO-ubiquitin chains containing SUMO-2 linked to Lys63-di-ubiquitin were found to play a major role in DNA repair. Despite some progress in understandin
Glycosaminoglycans (GAGs) are linear polysaccharide chains consisting of repeating disaccharide units and form proteglycans by covalently attaching to their core proteins. Chondroitin sulfate (CS) is a glycosaminoglycan with the disaccharide unit of beta-D-galactosamine (GalNAc) and beta-D-glucuronic acid (GlcA), and often modified with ester-linked sulfate at certain positions. Dermatan sulfate (DS) is a modified form of CS, in which a portion of D-glucuronate residues is epimerized to L-iduronates (IdoA). CS and DS are linked to serine residues in core proteins via a linkage tetrasaccharide formed by the transfer of xylose and three more residues [MD:M00057]. The assembly process of CS is initiated by transferring GalNAc residue to the linkage tetrasaccharide. The polymerization is catalyzed by bifunctional enzymes (chondroitin synthases) possessing both beta 1,3 glucuronosyltransferase and beta 1,4 N-acetylgalactosaminyltransferase activities [MD:M00058]. Chondroitin polymerization also ...
Chondroitin is a chondrin derivative. Types include: Chondroitin sulfate Dermatan sulfate Chondroitin at the US National Library of Medicine Medical Subject Headings (MeSH ...
Cross-species transplant in rhesus macaques is step toward diabetes cure for humans Friday, 19 October 2007 With an eye on curing diabetes, scientists at Washington University School of Medicine in St. Louis have successfully transplanted embryonic pig pancreatic cells destined to produce insulin into diabetic macaque monkeys - all without the need for risky immune suppression drugs that prevent rejection. The transplanted cells, known as primordia, are in the earliest stages of developing into pancreatic tissues. Within several weeks of the transplants, the cells became engrafted, or established, within the three rhesus macaque monkeys that received them. The cells also released pig insulin in response to rising blood glucose levels, as would be expected in healthy animals and humans. "The approach reduced the animals need for insulin injections and has promise for curing diabetes in humans," says senior investigator Marc Hammerman, M.D., the Chromalloy Professor of Renal Diseases in Medicine. ...
1DBG: Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution.
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Regeneration is, in simple terms, to re-grow damaged or lost parts of the body (e.g. cells, tissues and organs) and is a natural phenomenon occurring throughout the life of an organism. The regenerative capacity varies in the animal kingdom. Invertebrates have high regenerative capacity in contrast to higher vertebrates. This raises several fundamental questions related to the regeneration potential, evolutionary selection and its cellular and molecular mechanisms. An in-depth knowledge in regeneration is warranted to answer the fundamental questions that are still a challenge in regenerative medicine.. Glycosaminoglycans (GAGs) are known to be involved in various physiological processes. Of several GAG types galactosaminoglycans are the focus of this thesis. Galactosaminoglycans such as chondroitin sulfate/dermatan sulfate (CS/DS) are anionic linear polysaccharides covalently linked to core proteins so called proteoglycans (PGs), and form an integral part of both cell surface and ...
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Neuronal axons and their growth cones recognize molecular guidance cues within the local environment, forming axonal pathways to produce precise neuronal networks during nervous system development. Chondroitin sulfates (CS), carbohydrate chains on chondroitin sulfate proteoglycans, exhibit great structural diversity and exert various influences on axons and growth cones as guidance cues or their modulators; however, the relationship between their structural diversity and function in axonal guidance is not well known. To uncover the roles of CS in axonal guidance, artificially modified hybrid molecules: CS derivatives of biotinylated CS and lipid-derivatized CS, were used. The experiments with biotinylated CS suggest that the growing axons act on their environment, modifying CS, and rendering it more favorable for their growth. The experiments with lipid-derivatized CS demonstrated that growth cones distinguish types of CS with different unit contents and are likely to discriminate the structural
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One possibility is that these animals need particular cues from environmental bacteria that are not being provided in the lab," she said.. After UC Berkeley graduate student Arielle Woznica discovered that these bacteria initiated swarming, they collaborated with Clardys lab to track down the trigger: a protein constantly secreted by the bacteria, which they dubbed EroS.. They showed that EroS is a chondroitinase, an enzyme that degrades a specific type of sulfated molecule found in the extracellular matrix of S. rosetta that was previously thought to be exclusive to animals. They also found that if this enzymatic function was inhibited, swarming did not occur, and that chondroitinases from other aquatic bacteria reproduced the aphrodisiacal effects. As the teams investigate how EroS works, theyre continuing to explore the interactions between bacteria and choanoflagellates.. As for implications for animals like humans and their bacterial partners, the so-called microbiome, King said that "we ...
Hylauronidase will digest the hyaluronic acid region of the molecule which will unmask the access to BMP antigens. There are three regions of the molecule which we digest for, chondroitin sulfate (chondroitinase), keratin sulfate (keratinase), and hyaluronic acid (hyaluronidase). This is a different concept than unwinding proteins which have been crosslinked by aldehydes, in this case the actual physical structure of the molecule is blocking the antigen site. With some of these digestions you must also include proteinase inhibitors to prevent loss of proteoglycans which may what you are interested in marking. We use proteinase inhibitors with chondroitinase ABC digestion when we are doing IHC for Aggrecan, for instance. Patsy Ruegg -----Original Message----- From: Renton, Lousie, Mrs [mailto:[email protected]] Sent: Tuesday, June 26, 2001 6:04 AM To: [email protected] Subject: Haluronidase Dear all A colleague of mine has an IHC method for bone morphogenic proteins (on FFPE ...
... - Supports Healthy Joint Function* Supports Elasticity of Joint Tissues* Chondroitin Sulfate is a glycosaminoglycan and is a normal component of many body tissues including articular cartilage.* Our Chondroiti
Chondroitin Sulfate is a naturally-occurring compound found in the cartilage, bone, skin, cornea, and arterial walls, as well as tissues throughout the body. It plays an important role in the health, function, and mobility of joints, as well as the comfort and elasticity of joint tissues.** It also works with hyaluronic acid to support the shock-absorbing properties of aggrecan.**. Piping Rocks Chondroitin Sulfate supplements deliver 600 mg of this joint-nourishing nutrient in each serving. Adults take one (1) quick release capsule two to three times daily with meals for optimal nutritional support. It is important to note that, like glucosamine, it may take several weeks of supplementation before any noticeable difference is seen.. ...
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Current hydrogels based on chondroitin sulfate (ChS) generally lack the necessary strength and precise mechanical tunability. Addressing these limitations, covalent cross-linking has evolved to produce hydrogels with desirable properties. However, such a methodology always precludes injection and self-healin
It is not known what exactly to expect from a chondroitin overdose. However, as this eMedTV page discusses, an overdose is likely to cause stomach upset. This page covers other possible effects of an overdose on chondroitin, as well as treatment options.
As part of the study, the researchers tried to boost insulin production in the macaques by performing a second transplant. Four weeks after transplanting the embryonic pig cells, they implanted adult insulin-producing pig islet cells into the kidneys of the macaques. The embryonic cells primed the monkeys immune system to accept the mature islet cells, without using anti-rejection drugs, but their glucose control did not continue to improve.. Hammerman. Hammerman suspects this may be because they did not transplant enough adult pig islet cells or that placing them in the kidney is not an optimal location. As a comparison, the researchers also transplanted adult pig islet cells into a control group of diabetic macaques that did not receive the earlier embryonic pig cell transplants. Not surprisingly, the macaques immune systems attacked the foreign cells and eventually rejected those cells all together.. The new study builds on earlier research in rats, reported by the same group last year in ...
Chondroitin sulfate is one of the major constituents of cartilage. Chondroitin sulfate consists of repeating chains of molecules called mucopolysaccharides
Chondroitin sulfate is one of the major constituents of cartilage. Chondroitin sulfate consists of repeating chains of molecules called mucopolysaccharides
Chondroitin sulfate (CSA) is a mucopolysaccharide found in cartilage, tendons, and ligaments, where it is bound to proteins such as collagen and elastin. In our joints, it contributes to strength, flexibility and shock absorption. Current research indicates that supplemental CSA may help maintain proper joint function.
Researchers report the results of a trial which uncovered a benefit for supplementation with chondroitin sulfate in individuals with osteoarthritis of the hand.
Chondroitin Sulfate 500 mg Reviews and other Reviews of Nutritional Supplements and Merchants Plus Related Resources Including a 2017 Buying Guide. Healthy Learning for Healthy Living.
... was valued US$ XX Bn in 2018 and is expected to reach US$ XX Bn by 2026, at CAGR of XX% during forecast period.
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|p|Chondroitin occurs next to cartilage tissue in connective tissue, tendons and skin. In relatively small quantities, chondroitin is absorbed through anima
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Chondroitin is a naturally occurring substance formed of sugar chains. Chondroitin is believed to help the body maintain fluid and flexibility in the joints.
Chondroitinase offers us hope in two ways; firstly it allows some nerve fibers to regenerate and secondly it enables other nerves to take on the role of those fibers that cannot be repaired ...
Proteoglycans that modulate the activities of growth factors, chemokines, and coagulation factors regulate in turn the vascular endothelium with respect to processes such as inflammation, hemostasis, and angiogenesis. Endothelial cell-specific molecule-1 is mainly expressed by endothelial cells and regulated by pro-inflammatory cytokines (Lassalle, P., Molet, S., Janin, A., Heyden, J. V., Tavernier, J., Fiers, W., Devos, R., and Tonnel, A. B. (1996) J. Biol. Chem. 271, 20458-20464). We demonstrate that this molecule is secreted as a soluble dermatan sulfate (DS) proteoglycan. This proteoglycan represents the major form either secreted by cell lines or circulating in the human bloodstream. Because this proteoglycan is specifically secreted by endothelial cells, we propose to name it endocan. The glycosaminoglycan component of endocan consists of a single DS chain covalently attached to serine 137. Endocan dose-dependently increased the hepatocyte growth factor/scatter factor (HGF/SF)-mediated ...
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate ...
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Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars (N-acetylgalactosamine and glucuronic acid). It is usually found attached to proteins as part of a proteoglycan. A chondroitin chain can have over 100 individual sugars, each of which can be sulfated in variable positions and quantities. Chondroitin sulfate is an important structural component of cartilage and provides much of its resistance to compression. Along with glucosamine, chondroitin sulfate has become a widely used dietary supplement for treatment of osteoarthritis. Although chondroitin is used in dietary supplements as an alternative medicine to treat osteoarthritis and also approved and regulated as a symptomatic slow-acting drug for this disease (SYSADOA) in Europe and some other countries, it is technically neither a medicine nor a disease-modifying treatment. See Clinical effects below. It is commonly sold together with glucosamine. Chondroitin and glucosamine are also used in ...
One immediate result of our experiments is the ability to assign Ser999 as the principal site of chondroitin sulfate attachment to the NG2 polypeptide. On the basis of the apparent difference in molecular size between the NG2 core glycoprotein (300 kDa) and the intact proteoglycan (400-600 kDa smear) on SDS-PAGE, we had speculated that there might be several chondroitin sulfate chains associated with the core. The presence of at least two good consensus sequences for chondroitin sulfate attachment at Ser999 (EGSGD) and Ser1342 (SGLG, Nishiyama et al., 1991a) seemed consistent with this prediction. However, our current experiments with point mutants have shown that NG2/S1342A still contains chondroitin sulfate, whereas NG2/S999A is devoid of glycosaminoglycan, indicating that Ser1342 is not used as an attachment site. Thus, at least in this case, the E/DGSGE/D motif ( Zimmermann and Ruoslahti, 1989) is utilized, whereas the SGXG motif ( Bourdon et al., 1987) is not. This usage may highlight the ...
Chondroitin Sulfate comes from two main sources: • the bovine source (cattle), where the chondroitin is usually taken from the cattles trachea • the marine source, with the chondroitin typically sourced from shark cartilage. Good quality manufacturers will state on their packaging what type of chondroitin is used in their products. This is most important to people who have strong views over the type of chondroitin they use. For example, some may be happy to use supplements derived from shark cartilage but will not be willing to use supplements made from cow by-products - or vice-versa. In fact, the source of any dietary supplement should be one of your prime consideration. If you do not wish to use either animal or marine products to relieve your arthritis pain you may wish to try organic rosehip supplements, which offer a natural herbal alternative.. ...
Therapy. There are 13 Chapters to deeply display the global Chondroitin and Hyaluronic Acid market.. Chapter 1, to describe Chondroitin and Hyaluronic Acid Introduction, product scope, market overview, market opportunities, market risk, market driving force;. Chapter 2, to analyze the top manufacturers of Chondroitin and Hyaluronic Acid, with sales, revenue, and price of Chondroitin and Hyaluronic Acid, in 2015 and 2016;. Chapter 3, to display the competitive situation among the top manufacturers, with sales, revenue and market share in 2015 and 2016;. Chapter 4, to show the global market by regions, with sales, revenue and market share of Chondroitin and Hyaluronic Acid, for each region, from 2011 to 2016;. Chapter 5, 6, 7 and 8, to analyze the key regions, with sales, revenue and market share by key countries in these regions;. Chapter 9 and 10, to show the market by type and application, with sales market share and growth rate by type, application, from 2011 to 2016;. Chapter 11, Chondroitin ...
Supports healthy cartilage and joints Chondroitin sulfate is one of the most vital of the compounds in connective tissue which are responsible for building and supporting the ground substance of cartilage. As a nutritional supplement, this naturally occurring compound provides support for strong, healthy cartilage and joints. each vegetable capsule contains: chondroitin sulfate (bovine) 400 mg. (from 444 mg. of chondroitin sulfate (bovine)) vitamin C (as ascorbyl palmitate) 5 mg. (hypo-allergenic plant fiber added to complete capsule volume requirement) Directions: 3-12 capsules per day, in divided doses, with meals.
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There have been a number of clinical trials that have studied Glucosamine adn Chondroitin. One of the largest trials was conducted by the National Institutes of Health (NIH) and compared glucosamine HCl alone, chondroitin sulfate alone, the combination of glucosamine and chondroitin, Celebrex and a placebo (a substance containing no medications) in pateints with knee osteoartritis. In this large trial, there were no significant differences in outcome between the glucosamine and chondroitin sulfate groups than with the placebo. However, in several smaller clinicial trials in Europe, the formulation of glucosamine sulfate (not glucosamine HCl) has shown some possible benefits. The bottom line is that some patients with osteoarthritis do seem, individually, to report some benefits with these supplements.. The American College of Rheumatology 2012 guidelines do not recommend the use of glucosamine or chondroitin for the management of osteoarthritis. However, because there are very few known side ...
Sulfated complex carbohydrates in the adrenal medulla were labeled with 35SO4- after the administration of insulin to deplete catecholamine stores. Twenty-four hours later the left adrenal gland was perfused in situ with Lockes solution. Addition of acetylcholine to the perfusion solution resulted in a 12-20-fold increase in the release of sulfate-labeled nondialyzable material, together with the usual large increase in catecholamine secretion. Digestion of the sulfate-labeled macromolecular material with bacterial chondroitinases demonstrated that 65-80% of the radioactivity was in the form of chondroitin 4- and 6-sulfate. The remaining radioactivity was present in an N-sulfated mucopolysaccharide which was tentatively identified as heparan sulfate. The results of these experiments are consistent with our previous finding of sulfated mucopolysaccharides, consisting mostly of chondroitin 4- and 6-sulfate, in purified chromaffin granules from bovine adrenal medulla, and are discussed in terms of ...
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This study will determine whether glucosamine, chondroitin sulfate and/or the combination of glucosamine and chondroitin sulfate are more effective than placebo and whether the combination is more effective than glucosamine or chondroitin sulfate alone in the treatment of knee pain associated with osteoarthritis (OA) of the knee after six months of follow-up. These substances, marketed in the United States as dietary supplements, have been widely touted by the lay press and by anecdotal personal experience as effective in treating OA. To date, however, only a few small studies have been published in the worldwide literature. The study proposed herein has been carefully constructed to definitively determine the efficacy of these agents ...
Clinical experience likewise suggests that some dogs and cats may appreciably benefit from chondroitin administration and others not at all. It is difficult to anticipate which animals are most likely to respond. Given the potential for benefit and the extremely low risk of any adverse side effects, chondroitin supplementation is always recommended for animals with joint pain. Where a benefit is seen, doses of more expensive and potentially deleterious drugs may be reduced, including the NSAIDs (non steroidal anti-inflammatory drugs) such as aspirin, carprofen and meloxicam. Because chondroitin protects mucosal barriers, it may not only enhance pain relief in animals administered NSAIDS, but help protect their intestinal tracts from ulcerations which are occasionally seen as a side effect of NSAID use.. Research in laboratory animals suggests that chondroitin dissolved in water before administration is more effective than when administered within a capsule.. ...
Posted on Apr 7, 2015. To: Dr. Caesar DePaço, Summit Nutritionals International ®. Re: Droi-Kon ®. ________________. Dr. DePaço,. On behalf of myself and my K9 partner Uno, I would like to thank you for your assistance and guidance with your Droi-Kon ® Chondroitin.. This winter I noticed my K9 partner laying around a lot more and not being active, I figured it was just the winter blues. Then every day I noticed more signs, limping, frequent stretching, not wanting to go up or down the stairs in my house. I brought K9 Uno to the Vet, who stated he was very stiff and because of his age of 8, probably slightly arthritic. The Vet suggested a possible Chondroitin based product. Well, I knew exactly who to talk to about this.. In January, I contacted you and explained what was happening with K9 Uno, You said " Dont worry Chris, I know exactly how to help Uno"…well you did and I couldnt be more happy.. Due to your product, Droi-Kon ®, your instruction and help, by putting 1 tablespoon of ...
Chondroitin Sulfate is a glycosaminoglycan and is a normal component of many body tissues including articular cartilage.* Our Chondroitin is high potency and is derived from bovine cartilage.
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Product Name: Chondroitin Sulfate
Synonyms: chondroitin sulfuric acid, chonsurid
CAS: 9007-28-7
MF: (C14H21NO14
...     Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars (N-acetylgalactosamine
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Chondroitin Sulfate is classified as a biopolymer an acidic mucopolysaccharide. It is typically extracted from animal sources.. ...
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We are very grateful to our distinguished colleague for his constructive comments.1 However it seems that some keypoints of our study were grossly misunderstood.2 As clearly stated, the objective of the CONCEPT study was to confirm that chondroitin sulfate (CS) is superior to placebo (PLB) in the symptomatic treatment of osteoarthritis, with the addition of a Celebrex (CLB) arm, as requested by the European Medicines Agency, to provide an external validation and to better assess the relevance of the difference in pain relief observed between the CS and PLB arms. There was no intent to demonstrate a non-inferiority of CS versus Celebrex. In such a case, a non-inferiority margin for the comparison would be mentioned in the protocol, and the power calculation would be substantially different. The use of the word similar in describing Visual Analogue Scale (VAS) and Lequesne scoring … ...
We are very grateful to our distinguished colleague for his constructive comments.1 However it seems that some keypoints of our study were grossly misunderstood.2 As clearly stated, the objective of the CONCEPT study was to confirm that chondroitin sulfate (CS) is superior to placebo (PLB) in the symptomatic treatment of osteoarthritis, with the addition of a Celebrex (CLB) arm, as requested by the European Medicines Agency, to provide an external validation and to better assess the relevance of the difference in pain relief observed between the CS and PLB arms. There was no intent to demonstrate a non-inferiority of CS versus Celebrex. In such a case, a non-inferiority margin for the comparison would be mentioned in the protocol, and the power calculation would be substantially different. The use of the word similar in describing Visual Analogue Scale (VAS) and Lequesne scoring … ...
Hui, J.H., Chan, S.-W., Li, J., Goh, J.C.H., Li, L., Ren, X.F., Lee, E.H. (2007). Intra-articular delivery of chondroitin sulfate for the treatment of joint defects in rabbit model. Journal of Molecular Histology 38 (5) : 483-489. [email protected] Repository. https://doi.org/10.1007/s10735-007-9120- ...
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Shanghai-based Cactus Botanics has developed a new form of chondroitin sulfate, free-from impurities or adulteration and thoroughly tested for quality using four methodologies. Carol Cheow, general manager of Cactus Botanics, discu
I suffered some pretty severe joint trauma during a recent injury. Fortunately my recovery has gone exceptionally well, and Ive gotten back to a pretty highly active lifestyle. However I do have varying degrees pain throughout the day. Id like to try glucosamine/chondroitin in addition to my exercises to see if it helps improve joint function and pain, but there doesnt appear to be any available data on the safety of either glucosamine or chondroitin sulfate during breastfeeding. :hmmm
The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011 ...
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|p|Commonly taken as a complex, glucosamine, chondroitin and MSM are compounds that can work together to support healthy muscles and joints, working to relieve painful conditions such as osteoarthritis.|/p| |p|Glucosamine and chondroitin are produced nat
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Refill Bag x 6 - 360 day supply. No longer using jars, price reduction.60 Tablespoons. $159.95. If you are a repeat customer and buying chondroitin separately (not in a combo), as a thank you, please apply the coupon ChonRocks to get 10% off of your Chondroitin purchase when you check out ...
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4R1L: Crystal structure of a Hypothetical Acyl-CoA ligase (BT_0428) from Bacteroides thetaiotaomicron VPI-5482 at 2.42 A resolution
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Glucosamine + Chondroitin with MSM Healthy cartilage strength and resilience Sulfur-containing methylsulfonylmethane (MSM) is included in this glucosamine and chondroitin combination to provide an enhanced spectrum of nutrients for optimal cartilage matrix composition, connective tissue strength, and joint comfort. Glucosamine promotes the synthesis of the glycosaminoglycan chondroitin sulfate. Chondroitin sulfate is responsible for building the ground substance of cartilage, molecules known as proteoglycans. In addition, chondroitin sulfate may maintain healthy enzyme activity. An important role of sulfur from MSM is to enhance the structure and integrity of proteoglycans. Pure Encapsulations Glucosamine and Chondroitin with MSM offers highly purified constituents for healthy cartilage formation and joint movement ease. Supplement Facts: Amount Per Serving each vegetable capsule contains: glucosamine HCl (crab, shrimp), 99% 300 mg. chondroitin sulfate (bovine), 95% 300 mg. MSM
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1. Busci L & Poor G: Efficacy and tolerability of oral chondroitin sulfate as a symptomatic slow-acting drug for osteoarthritis (SYSADOA) in the treatment of knee osteoarthritis. Osteoarthritis Cartilage 1998; 6(supplement A):31-36. 2. Morrison LM & Enrick NL: Coronary heart disease: reduction of death rate by chondroitin sulfate-A. Angiology 1973: 24(5): 269-287. 3. Tallia A & Cardone D: Asthma exacerbation associated with glucosamine-chondroitin supplement. J Am Board Fam Pract 2002; 15(6):841-848. 4. Uebelhart D, Thonar E & Delmas P: Effects of oral chondroitin sulfate on the progression of knee osteoarthritis: a pilot study. Osteoarthritis Cartilage 1998; 6 (supplement A):39-46. 5. Verbruggen G, Goemaere S & Veys E: Chondroitin sulfate: S/MOAD (structure/disease modifying anti-osteoarthritis drug) in the treatment of finger joint OA. Osteoarthritis Cartilage 1998; 6(suppl A): 37-38. 6. Product Information: Glucosamine hcl chondroitin sulfate oral tablets. Puritans Pride, Bohemia, NY, 2006. ...
|p|Sulfation pattern within chondroitin sulfate (CS) glycosaminoglycan (GAG) chains is an important post-translational modification that regulates their interaction with proteins. In this context, development of highly efficient and reproducible analytical methods for the investigation of CS sulfation patterns is of high necessity. In this study we report a novel method for straightforward determination of N-acetylgalactosamine (GalNAc) sulfation sites in chondroitin sulfate disaccharides. Our protocol involves combining fully automated chip-based nanoelectrospray (nanoESI) for analyte infusion and ionization in negative ion mode with multistage (MSn) collision-induced dissociation (CID) high capacity ion trap (HCT) mass spectrometry for generation of sequence ions diagnostic for identification of sulfate ester group position within GalNAc residues. The feasibility of this approach is here demonstrated on chondroitin 6-O-sulfate and chondroitin 4-O-sulfate disaccharides.
MalaCards based summary : Multiple Joint Dislocations, Short Stature, Craniofacial Dysmorphism, and Congenital Heart Defects, also known as multiple joint dislocations-short stature-craniofacial dysmorphism-congenital heart defects syndrome, is related to larsen-like syndrome and larsen-like syndrome, lethal type, and has symptoms including joint laxity, hypertelorism and low-set ears. An important gene associated with Multiple Joint Dislocations, Short Stature, Craniofacial Dysmorphism, and Congenital Heart Defects is B3GAT3 (Beta-1,3-Glucuronyltransferase 3), and among its related pathways/superpathways are Glycosaminoglycan metabolism and Chondroitin sulfate/dermatan sulfate metabolism. Affiliated tissues include heart, bone and skin, and related phenotype is Increased shRNA abundance (Z-score > 2) ...