BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
Microencapsulation of recombinant cells is a novel promising approach to tumor therapy in which therapeutic protein is sustainable and long-term delivered by microencapsulated cells. The semi-permeable membrane of microcapsule can protect cell from hosts immune rejection, increase the chemical stability of therapeutic protein and circumvent the problems of toxicity, limited half-lives and variation in circulating levels. Endostatin, a potent and specific angiogenesis inhibitor, could suppress the growth of primary and metastatic lesions in multiple murine tumor models. In this paper, APA microcapsules with high strength kept intact over 35 days and recombinant CHO cells kept the rapid proliferation viability and the continuous endostatin-expression function. The study of tumor treatment showed that the implantation of microencapsulated recombinant CHO cells decreased the neovascularization of tumor tissue by 59.4% and inhibited the B16 melanoma growth by 77.4%. Twenty days after tumor cell ...
Monoclonal antibodies specific for epitopes on the perstussigen molecule, s-1, were developed by the Fetuin-ELISA test, radioimmunoassays and Western Blott(27). Hybridoma fluids already established for specificity on s-2, s-4 and the undigested molecule were exposed to pertussigen to detect the neutralization of agglutination to Chinese Hamster Ovary (CHO) cells. The results showed that no appreciable neutralization occurred. Many more monoclonals need to be developed and tested to find the critical epitope or epitopes which cause agglutination to CHO cells. This research may provide a clue to the specific antigenic site which causes toxicity attributed to whooping cough in humans ...
BioAssay record AID 34248 submitted by ChEMBL: Displacement of [3H]NECA from human adenosine A2A receptor in stably transfected CHO cells.
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
serumm free culture of adherent cho cells - posted in Tissue and Cell Culture: Hi all, i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media for 72-96h. i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions which medium i can use to sustain 72-96h time? thnx
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. Biotechnol Bioeng. 2019 Aug 12 ...
Glenmark signs full commercial-use license for Horizons gene-edited CHO cells Cambridge, UK, 30 September 2019: Horizon Discovery Group plc (LSE: HZD) (Horizon), a global leader in the application of gene editing and gene modulation for cell line engineering, today announced the full commercial licensing to Glenmark Pharmaceuticals, a global innovative pharmaceutical company, of its gene-edited Glutamine Synthetase (GS) knockout Chinese Hamster Ovary (CHO) K1 cell line. Terms of the agreement were based on stringent evaluation of the cell line by Glenmark to assess its suitability for adoption into the Companys biomanufacturing processes. Martin Bertschinger, Deputy Director of Cell Sciences, Glenmark, explained: After extensive evaluation, Horizons GS knockout CHO K1 cell line demonstrated consistently impressive performance. We generated clones with high levels of productivity and a favorable stability profile relative to our previous system. Incorporating this technology into our ...
Phenotypic variations in the cells arise from changes in their DNA, including differences in methylation patterns. From analyzing CHO cell lines, researchers found that the transcriptome of each subclone also had a significant number of individual changes and that such changes indicate that epigenetic regulation is a hidden but important player in cell line development.
We have investigated the role of HIF-1 in the cellular response to redox modulation via the inhibition of oxidative phosphorylation. We demonstrate that manipulation of redox in air, achieved by inhibiting cytochrome oxidase with cyanide, induces HIF-1 mediated transcription in wild-type CHO and HT1080 human tumour cells but not in CHO cells deficient in the oxygen responsive, HIF-1alpha sub-unit of HIF-1. Hypoglycaemia attenuates cyanide-mediated transcription in non-transformed HIF-1 wild-type CHO cells but not the human tumour derived cell line. Cells lacking either HIF-1alpha, or the second composite sub-unit of HIF-1, HIF-1beta, were markedly more sensitive to the combined stress of perturbed redox and hypoglycaemia than wild-type cells. As such conditions together with hypoxia are prevalent in tumours, these data suggest that HIF-1 may have a protective role in adaptation to the tumour micro-environment. In support of this we demonstrate that HIF-1alpha deficient cells are less tumorigenic than
We have investigated the role of HIF-1 in the cellular response to redox modulation via the inhibition of oxidative phosphorylation. We demonstrate that manipulation of redox in air, achieved by inhibiting cytochrome oxidase with cyanide, induces HIF-1 mediated transcription in wild-type CHO and HT1080 human tumour cells but not in CHO cells deficient in the oxygen responsive, HIF-1alpha sub-unit of HIF-1. Hypoglycaemia attenuates cyanide-mediated transcription in non-transformed HIF-1 wild-type CHO cells but not the human tumour derived cell line. Cells lacking either HIF-1alpha, or the second composite sub-unit of HIF-1, HIF-1beta, were markedly more sensitive to the combined stress of perturbed redox and hypoglycaemia than wild-type cells. As such conditions together with hypoxia are prevalent in tumours, these data suggest that HIF-1 may have a protective role in adaptation to the tumour micro-environment. In support of this we demonstrate that HIF-1alpha deficient cells are less tumorigenic ...
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
name:조은석; Cho, Eun Suk; cho, eun suk; cho eun suk; eun suk cho; eun suk, cho; Cho, Eun Suk; Cho Eun Suk; Eun Suk Cho; Eun Suk, Cho
Feichtinger J., Hernandez I., Fischer C., Hanscho M., Auer N., Hackl M., Jadhav V., Baumann M., Krempl P.M., Schmidl C., Farlik M., Schuster M., Merkel A., Sommer A., Heath S., Rico D., Bock C., Thallinger G.G., Borth N. (2016) Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time. Biotechn. Bioeng. 113:10:2241-2253. DOI: 10.1002/bit.25990. Gludovacz E., Maresch D., Bonta M., Szöllösi H., Furtmüller P.G., Weik R., Altmann F., Limbeck A., Borth N., Jilma B., Boehm T. (2016) Characterization of recombinant human diamine oxidase (rhDAO) produced in Chinese Hamster Ovary cells. J Biotechnol. 227, 120-130. Klanert G., Jadhav V., Shanmukam V., Diendorfer A.m Karbiener M., Scheideler M., Bort JH., Grillari J., Hackl M., Borth N. (2016) A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines. J Biotechnol. DOI 10.1016/j.jiotec.2016.03.022. Priola J.J., Calzadilla N., Baumann M., Borth N., Tate ...
Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
By Tom Fagan. In recent years, Aβ oligomers have grabbed the research limelight. Bigger than monomers, but not full-fledged fibrils, Aβ oligomers are touted as the most toxic forms of the peptide. But do they really exist in the brain? If so, what form do they take?. Oligomers burst onto the scene over 15 years ago, when researchers in Dennis Selkoes lab at Brigham and Womens Hospital, Boston, found that Chinese hamster ovary (CHO) cells transfected with the amyloid-β precursor protein gene secreted Aβ oligomers (see Podlisny et al., 1995). Working in the lab, Dominic Walsh discovered that these natural oligomers were toxic to neurons. CHO-derived monomers were innocuous, but dimers, trimers, and larger species blocked synaptic function when injected into rat brain (see ARF related news story). Collaborating with Walsh and Selkoe, Karen Ashes group at the University of Minneapolis, Minnesota, subsequently found that low doses of these oligomers acutely suppress learning and memory when ...
Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
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MEMORY BASED UP CHO QUESTION PAPER||UP CHO ANSWER KEY 2021 SHIFT FIRST||IMPORTANT MCQ CHO EXAM UPCHO MODEL TEST PART-2 SERIES||UP CHO MODE TEST MCQ||C...
CHO-Anti-Human TSHR F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TSHR F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
CHO-Anti-Human TGF beta 2 scFv stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TGF beta 2 scFv gene to allow expression of the scFv. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Major advantages of perfusion are high cell numbers and high total production in a relatively small size bioreactor. Moreover, perfusion is optimal when the product of interest is unstable or if the product yield is low. On the other hand, disadvantages are for example technical challenges originating from non-robust cell separation devices as well as sterility concerns from the more complex set-up needed.. In the present work, the use of a WAVE Bioreactor™ system 20/50 in perfusion mode with10 L disposable Cellbag™ bioreactors customized with two dip tubes in combination with disposable hollow fiber filters as external cell separating devices were investigated. A comparison between Alternating Tangential Flow (ATF) and Tangential Flow Filtration (TFF) was performed using a recombinant CHO cell line producing a monoclonal antibody (mAb) as a model system. ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHO(BRI/rcTA)) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHO(BRI/rcTA) pools producing high levels of recombinant proteins. Volumetric productivities of up to 900mg/L were reproducibly achieved for a Fc fusion protein and up to 350mg/L for an antibody after 14days post-induction in non-optimized fed-batch cultures. In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. ...
We have investigated whether the presence of a DNA repair enzyme, 06-methylguanine-DNA-methyltransferase (MGMT), affects the nature of spontaneous mutations in a mammalian cell line. We compared spontaneous mutations in the adenine phosphoribosyl transferase gene of a Chinese hamster ovary (CHO) cell line that expressed 14,000 MGMT molecules/cell with those in the parental CHO cells lacking this DNA repair activity. The mutation rate/cell/generation of the two CHO cell lines did not differ significantly. However, DNA sequence analysis of spontaneous mutations in the MGMT-proficient CHO cell line revealed a complex picture. No significant difference from the parental CHO cells was found in the number or type of deletions, frame-shifts, multiple substitutions, or insertions. The frequency of G:C to T:A transversions was elevated in MGMT-proficient CHO cells. Expression of the enzyme considerably reduced G:C to A:T transitions (25% versus 8.3%). This latter result is the first evidence that this ...
In a previous blog Strategies for Improving Antibody Production in CHO Cells three areas were identified where antibody production can be improved. In part one of the series titled Utilizing Gene Synthesis to Improve Antibody Production in CHO Cells, we looked at gene synthesis as an alternative to classic cloning that offers a precise way to create a gene. In part two of the series titled Strategies for Enhancing Media to Improve Antibody Production in CHO Cells, we examined the use of new media supplements to improve cell growth and productivity. In part three we will look at ways perfusion bioreactors can improve antibody production in CHO cell manufacturing.. In the biopharmaceutical industry there is an ongoing discussion about manufacturing capacity vs. demand for biologic drugs. Recently there has been increased concern because several biologic drugs have entered the final phases of the clinical pipeline. If many of these drugs receive approval, capacity could quickly become in ...
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. The Chinese hamster had been used in research since 1919 where they were used in place of mice for typing pneumococci. They were subsequently found to be excellent vectors for transmission of kala-azar (a.k.a. visceral leishmaniasis), facilitating leishmania research. In 1948, the Chinese hamster was first used in the United States for breeding in research laboratories. In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganians laboratory at the Boston Cancer Research Foundation and used it to ...
Kukkonen JP; G-protein-dependency of orexin/hypocretin receptor signalling in recombinant Chinese hamster ovary cells.; Biochem Biophys Res Commun, 2016 PubMed Europe PMC Scholia ...
Kukkonen JP; G-protein-dependency of orexin/hypocretin receptor signalling in recombinant Chinese hamster ovary cells.; Biochem Biophys Res Commun, 2016 PubMed Europe PMC ...
Glycosylation modulates growth, maintenance, and stress signaling processes. Consequently, altered N-glycosylation is associated with reduced fitness and disease. Therefore, expanding our understanding of N-glycans in altering biological processes is of utmost interest. Herein, clustered regularly interspaced short palindromic repeats/caspase9 (CRISPR/Cas9) technology was employed to engineer a glycosylation mutant Chinese Hamster Ovary (CHO) cell line, K16, which expresses predominantly hybrid type N-glycans. This newly engineered cell line enabled us to compare N-glycan effects on cellular properties of hybrid type N-glycans, to the well-established Pro´5 and Lec1 cell lines, which express complex and oligomannose types of N-glycans, respectively. Lectin binding studies revealed the predominant N-glycan expressed in K16 is hybrid type. Cell dissociation and migration assays demonstrated the greatest strength of cell-cell adhesion and fastest migratory rates for oligomannose N-glycans, and ...
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells. (1981). Subject Strain Bibliography 1981. 19 ...
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In interphase Chinese hamster ovary (CHO) cells, the centrosome is attached to the nucleus very firmly. This nuclear-centrosome complex is isolated as a coheren
BGI, the giant genomics institute located in Shenzhen, and GT Life Sciences of San Diego have published their collaborative study on the genomic sequence of the Chinese hamster ovary (CHO) K1 cell line in Nature Biotechnology. Over 70% of the recombinant therapeutic proteins sold today are manufactured using mammalian cells, primarily CHO cell lines. GT Life Sciences uses a metabolic modeling platform to design new products and processes for the life sciences field. It says a better understanding of the genome will speed development of new recombinant protein therapies. More details.... Share this with colleagues:   var switchTo5x=true;stLight.options({publisher:d7871f5b-67bc-4d30-b66f-1465d0b97213});
Previous findings have established G-6-P as an important mediator promoting HKII dissociation from mitochondria to cytosol (White and Wilson, 1990; Aleshin et al., 1998; Sebastian et al., 1999). In CHO cells overexpressing HKII, but not HKI, we found that glucose removal was associated with a delay in the subsequent rate of glucose phosphorylation (when Cyto B was present to block glucose efflux; John et al., 2011). Based on our prior detailed analysis in CHO cells (John et al., 2011), we attribute this delay to the activation of glycogenolysis when glucose was removed, which elevated G-6-P levels to both inhibit HKII and promote its dissociation from mitochondria. This interpretation was supported by our findings that IAA, which elevates G-6-P by inhibiting glycolysis distally (Fig. S2), caused HKII to dissociate from mitochondria in intact CHO cells with glucose present, and that exogenous G-6-P accelerated dissociation of HKs from mitochondria in permeabilized CHO cells (John et al., ...
The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
We have isolated cis-diamminedichloroplatinum(II) (CDDP)-resistant variants, C/CDP-1 and C/CDP-2, from a Chinese hamster ovary (CHO) cell line after a stepwise exposure to increasing concentrations of CDDP, and a CDDP-sensitive revertant, R-1, from C/CDP-2 after continuous incubation for 5 months in the absence of CDDP. C/CDP-1 and C/CDP-2 showed 7- and 10-fold higher resistance to CDDP, respectively, compared to CHO cells. C/CDP-2 was cross-resistant to carboplatin, l-phenylalanine mustard (melphalan), and CdSO4, but not to other anticancer agents. Alkaline elution of DNA showed an increased amount of DNA interstrand cross-linking formation in CHO cells, but not in C/CDP-2 cells, when CHO and C/CDP-2 cells were cultured with CDDP. By contrast, alkaline elution of DNA showed increased formation of DNA cross-links when nuclei of C/CDP-2 cells were treated with CDDP. The activity of glutathione S-transferase (GST) of C/CDP-1 and C/CDP-2 was 4- and 6-fold higher than that of CHO cells, ...
The technology is set to heighten the companys GPEx expression platform through the utilization of a glutamine synthase knock-out Chinese hamster ovary cell line.
Các gói cho thuê âm thanh của công ty cho thuê âm thanh ánh sáng: 1. Gói cho thuê âm thanh hội thảo - hội nghị (GÓI SỐ 1) của công ty cho thuê âm thanh ánh sáng: Gói cho thuê âm thanh hội thảo này được dùng cho các sự kiện âm thanh hội thảo hội nghị nhỏ trong phòng. https://images-blogger-opensocial.googleusercontent.com/gadgets/proxy?url=http%3A%2F%2Fsukienmattroi.com%2Fwp-content%2Fuploads%2F2017%2F03%2Fgoi-so-11.jpg&container=blogger&gadget=a&rewriteMime=image%2F* Giá gói cho
Activin A, Human, Recombinant, CHO Cells Activin A, Human, Recombinant, is a member of the TGF-β superfamily that is involved in the negative regulation of B lineage lymphocytes. A disulfide-linked homodimer of two 116-a.a. βA subunits. - Find MSDS or SDS, a COA, data sheets and more information.
Over the past decades, the increase in maximal cell numbers for the production of mammalian derived biologics has been in a large part due to the development of optimal feeding strategies. Engineering of the cell line is one of probable approaches for increasing cell numbers in bioreactor. We have demonstrated that the over-expression of the c-myc gene in immortalised CHO cells can increase proliferation rate and maximal cell density in batch culture compared to the control. The changes were attributed to a rapid transition into S-phase from a shortened duration of G1 phase and to the uncoupling of cell size from cell proliferation. To achieve the |70% increase in maximal cell density without additional supply of nutrients the cells underwent an overall reduction of 14% in size as well as a significant decrease in glucose and amino acid consumption rate. Consequently, the total biomass accumulation did not show a significant change from the control. The amount of hSEAP-hFc activity of the over
Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells Academic Article ...
Wong, D.C.F., Wong, K.T.K., Nissom, P.M., Yap, M.G.S., Heng, C.K. (2006). Targeting early apoptotic genes in batch and fed-batch CHO cell cultures. Biotechnology and Bioengineering 95 (3) : 350-361. [email protected] Repository. https://doi.org/10.1002/bit. ...
LinkedIn: Chinese Hamster Ovary cells, known as CHO, play an important role in modern medicine. NISTs new CHO peptide library will enable better production of treatments for psoriasis, cancer, hemophilia and leukemia.
In this application note Maxcyte review the rapid development of a high titer protein expression system in CHO suspension cells using a proprietary scalable electroporation technology. Optimised protein expression protocols provide the ability to load cells with greater quantities of DNA relative to the standard CHO protocol. As a result, average protein expression per cell is increased.
One Liter of Irvine Scientific BalanCD™ CHO Growth A Medium for use with the CELLine bioreactor flask |p| BalanCD CHO™ Growth A. It is a chemically-defined, no animal- derived media specifically designed for used in CHO cell applications. It
There are now several examples of single G protein-coupled receptors to which binding of specific agonists causes differential effects on the associated signaling pathways. The dopamine D2 receptor is of special importance because the selective activation of functional pathways has been shown both in vitro and in situ.
BalanCD® CHO Feed 3 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...
BalanCD® CHO Feed 1 is a chemically-defined, animal component-free feed medium designed to increase process yields of antibodies and recombinant proteins in Chinese Hamster Ovary (CHO) cells in fed-batch mode. This formulation was developed using Irvine Scientifics Rational Culture Media Design® strategy to achieve enhanced performance of growth and production in fed-batch culture ...