TY - JOUR. T1 - Immunobiology of monocytes and macrophages during Chlamydia trachomatis infection. AU - Nielsen, Mads Lausen. AU - Christiansen, Gunna. AU - Poulsen, Thomas Bouet Guldbæk. AU - Birkelund, Svend. PY - 2019/3/1. Y1 - 2019/3/1. N2 - Infections caused by the intracellular bacterium Chlamydia trachomatis are a global health burden affecting more than 100 million people annually causing damaging long-lasting infections. In this review, we will present and discuss important aspects of the interaction between C. trachomatis and monocytes/macrophages.. AB - Infections caused by the intracellular bacterium Chlamydia trachomatis are a global health burden affecting more than 100 million people annually causing damaging long-lasting infections. In this review, we will present and discuss important aspects of the interaction between C. trachomatis and monocytes/macrophages.. KW - Chlamydia trachomatis. KW - Macrophages. KW - Monocytes. UR - ...
Chlamydia trachomatis serovar E is one of the most common bacterial sexually transmitted pathogens. Since it is an obligate intracellular bacterium, efficient colonization of genital mucosal epithelial cells is crucial to the infectious process. Serovar E elementary bodies (EB) metabolically radiolabeled with 35S-Cys-Met and harvested from microcarrier bead cultures, which significantly improves the infectious EB-to-particle ratio, provided a more accurate picture of the parameters of attachment of EB to human endometrial epithelial cells (HEC-1B) than did less infectious 14C-EB harvested from flask cultures. Binding of serovar E EB was (i) equivalent at 35 and 4 degrees C, (ii) decreased by preexposure of EB to heat or the topical microbicide C31G, (iii) comparable among common eukaryotic cell lines (HeLa, McCoy), and (iv) significantly increased to the apical surfaces of polarized cells versus nonpolarized cells. In parallel experiments with C. trachomatis serovar L2, serovar E attachment was ...
In an attempt to establish a model of chlamydial ascending salpingitis and infertility, three inbred strains of mice, C3H/HeN (H-2k), C57BL/6N (H-2b), and BALB/cAnN (H-2d), were inoculated intravaginally with 3 x 10(7) inclusion-forming units of the Chlamydia trachomatis mouse pneumonitis biovar. Mice mated 6 weeks following inoculation were found to have a significant decrease in fertility rate compared with the control groups, as shown by a reduction in the number of pregnant mice and a decrease in the number of embryos. ...
In this work, the geometrical characteristics of two different types of cells observed with Electron Microscopy were analysed. The nuclear envelope of Wild-type HeLa cells and Chlamydia trachomatis-infected HeLa cells were automatically segmented and then modelled against a spheroid and converted to a two-dimensional surface. Geometric measurements from this surface and the volumetric nuclear envelope were extracted to compare the two types of cells. The measurements included the nuclear volume, the sphericity of the nucleus, its flatness or spikiness. In total 13 different cells were segmented: 7 Wild-type and 6 Chlamydia trachomatis-infected. The cells were statistically different in the following measurements. Wild-type HeLa cells have greater volumes than that of Chlamydia trachomatis-infected HeLa cells and they are more spherical as Jaccard index suggests. Standard deviation (σ), and range of values for the nuclear envelope, which shows the distance of the highest peaks and deepest ...
The obligate intracellular bacterium Chlamydia trachomatis is a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatis replicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. ...
Objectives: Sampling of both the cervix and urine increases the chance of detection of Chlamydia trachomatis compared with sampling either site alone. We determined the effect of combining urine and cervical swab specimens in the clinic setting on the sensitivity of C trachomatis polymerase chain reaction (PCR) testing.. Methods: For each of 100 women attending a genitourinary medicine clinic with high likelihood of genital C trachomatis infection, one endocervical swab was placed in transport medium and another in one of two aliquots of first void urine. Four PCR assays per patient (urine + swab, swab alone, and urine alone both pre- and post-freeze-thawing) were processed by automated C trachomatis PCR (Cobas, Amplicor). An inhibition control was included with each assay to identify specimens containing PCR inhibitors.. Results: 71% of women were Amplicor C trachomatis PCR positive (according to the results of at least one specimen). PCR test results were concordant for 95/100 patients, and of ...
The present disclosure relates to a solid phase immunoassay for the detection of Chlamydia trachomatis antigens in a clinical specimen, wherein the Chlamydia trachomatis antigens to be determined are coated or adsorbed on the solid phase.
Chlamydia trachomatis Serovar D ATCC ® VR-885™ Designation: Trachoma type D strain UW-3/Cx Application: Sexually Transmitted Disease Research
article{2075419, abstract = {BACKGROUND: In many developing countries, little is known about the prevalence of genital Chlamydia trachomatis infections and complications, such as infertility, thus preventing any policy from being formulated regarding screening for C. trachomatis of patients at risk for infertility. The objective of the present study was to determine the prevalence of C. trachomatis and evaluate the diagnostic utility of serological markers namely anti-C. trachomatis IgG and IgA antibodies in women attending an infertility clinic. METHODS: Serum and vaginal swab specimens of 303 women presenting with infertility to the infertility clinic of the Kigali University Teaching Hospital and 312 fertile controls who recently delivered were investigated. Two commercial species-specific ELISA were used to determine serum IgG and IgA antibodies to C. trachomatis and vaginal swabs specimens were tested by PCR. Hysterosalpingography (HSG) was performed in subfertile women. RESULTS: The PCR ...
Copyright © 2017 American Society for Microbiology. All Rights Reserved. Chlamydia trachomatis infection is the most prevalent bacterial sexually transmitted infection and can cause significant reproductive morbidity in women. There is insufficient knowledge of C. trachomatis-specific immune responses in humans, which could be important in guiding vaccine development efforts. In contrast, murine models have clearly demonstrated the essential role of T helper type 1 (Th1) cells, especially interferon gamma (IFN-γ)-producing CD4+ T cells, in protective immunity to chlamydia. To determine the frequency and magnitude of Th1 cytokine responses elicited to C. trachomatis infection in humans, we stimulated peripheral blood mononuclear cells from 90 chlamydia-infected women with C. trachomatis elementary bodies, Pgp3, and major outer membrane protein and measured IFN-γ-, tumor necrosis factor alpha (TNF-α)-, and interleukin-2 (IL-2)-producing CD4+ and CD8+ T-cell responses using intracellular ...
AIMS--To examine the role of Chlamydia trachomatis in ectopic pregnancy by detection of DNA in archival salpingectomy specimens, and in their preceding cervical specimens and endometrial biopsies, by using the polymerase chain reaction (PCR). METHODS--Archival paraffin embedded salpingectomy tissues (n = 48) from 37 women with ectopic pregnancy were examined for the presence of C trachomatis plasmid and omp1 DNA by PCR. In addition, preceding cervical specimens (n = 58) stored either as cervical cell suspensions or as archival cervical smears, and preceding endometrial biopsies (n = 18), taken 0-5.8 years before the ectopic pregnancy, were examined by PCR for the presence of C trachomatis. RESULTS--C trachomatis DNA was detected in only one of the 48 salpingectomy specimens from 37 women. However, in six of the 37 women, C trachomatis DNA was detected in the genital specimens (cervix and/or endometrial) taken before salpingectomy. C trachomatis infections were mostly found in endometrial or ...
A method is described in which smears on slides, which had been examined previously in a direct fluorescence antibody (DFA) test for Chlamydia trachomatis, were tested by the polymerase chain reaction (PCR). Twenty four (73%) of 33 smears which contained fewer than 10 elementary bodies when examined by the DFA test were positive by the PCR. Of the nine negative smears, seven contained only one or two elementary bodies. However, single elementary bodies were detected by the PCR in seven of the 24 positive samples. Fifteen smears were negative by both methods. The ability to detect small numbers of elementary bodies by the PCR and its specificity for negative smears indicates its potential for retrospective analysis of stored, archival smears on slides.. ...
Detection of Chlamydia trachomatis antigens in first-void urine to identify asymptomatic male carriers.: Early morning first-void urine collected from 279 sexua
With more than 90 million new cases annually, Chlamydia trachomatis is the most common sexually transmitted bacterial disease. Untreated endocervical C. trachomatis infections can cause pelvic inflammatory disease (PID), a disorder of the endometrium, fallopian tubes, and adjacent structures that occurs after ascension of the bacterium from the lower to upper genital tract. Adverse outcomes secondary to C. trachomatis-induced PID include tubal infertility, ectopic pregnancy, and chronic pelvic pain. Vaccine development has been identified as essential for control of C. trachomatis infections, and current evidence suggests that an effective vaccine will likely be based on several C. trachomatis antigens. Experimental models of infection have identified HSP60, major outer-membrane protein (MOMP), outer membrane protein 2 (OMP2), and polymorphic membrane protein D (PmpD) as promising vaccine candidates. A prospective study of Kenyan commercial sex workers found that production of interferon-gamma ...
NATtrol Chlamydia Trachomatis serotype D External Run Control is ready to use, inactivated full process controls designed to evaluate performance of molecular tests. They can be used for verification of assays, training of laboratory personnel and to monitor assay-kit lot performance.
The Gram-negative, intracellular bacterium Chlamydia trachomatis causes acute and chronic urogenital tract infection, potentially leading to infertility and ectopic pregnancy. The only partially characterized cytotoxin CT166 of serovar D exhibits a DXD motif, which is important for the enzymatic activity of many bacterial and mammalian type A glycosyltransferases, leading to the hypothesis that CT166 possess glycosyltransferase activity. CT166-expressing HeLa cells exhibit actin reorganization, including cell rounding, which has been attributed to the inhibition of the Rho-GTPases Rac/Cdc42. Exploiting the glycosylation-sensitive Ras(27H5) antibody, we here show that CT166 induces an epitope change in Ras, resulting in inhibited ERK and PI3K signaling and delayed cell cycle progression. Consistent with the hypothesis that these effects strictly depend on the DXD motif, CT166 with the mutated DXD motif causes neither Ras-ERK inhibition nor delayed cell cycle progression. In contrast, CT166 with the
Our research addresses the molecular basis of the interactions between disease-causing bacteria and their mammalian hosts. In particular, we study the intracellular biology of the obligate intracellular bacterium Chlamydia trachomatis. C.trachomatis is the leading bacterial agent of sexually transmitted disease worldwide and in developing nations causes blindness (trachoma), a neglected tropical disease.. Chlamydia forces its own entry into eukaryotic cells and forges a replicative niche within a specialised membrane-bound compartment (an inclusion), reprogrammed by the bacteria to mimic a host organelle. The inclusion remains segregated from the cellular endocytic pathway but is able to selectively engage with the secretory pathway.. Like some other Gram-negative bacteria including Salmonella and enteropathogenic E.coli, Chlamydia encodes a type III secretion system (T3SS), a sophisticated macromolecular assembly that spans the bacterial envelope and translocates virulence effector proteins ...
Mouse monoclonal antibody raised against Chlamydia trachomatis LPS. Chlamydia trachomatis elementary bodies, L2 serovar. (MAB6165) - Products - Abnova
The obligate intracellular bacterium Chlamydia trachomatis is the most common sexually-transmitted pathogen in the developed world, |100 million persons are inf...
Objective: To measure the coverage and uptake of systematic postal screening for genital Chlamydia trachomatis and the prevalence of infection in the general population in the United Kingdom. To investigate factors associated with these measures. Design: Cross sectional survey of people randomly selected from general practice registers. Invitation to provide a specimen collected at home. Setting: England. Participants: 19 773 men and women aged 16-39 years invited to participate in screening. Main outcome measures: Coverage and uptake of screening; prevalence of chlamydia. Results: Coverage of chlamydia screening was 73% and was lower in areas with a higher proportion of non-white residents. Uptake in 16-24 year olds was 31.5% and was lower in men, younger adults, and practices in disadvantaged areas. Overall prevalence of chlamydia was 2.8% (95% confidence interval 2.2% to 3.4%) in men and 3.6% (3.1% to 4.9%) in women, but it was higher in people younger than 25 years (men 5.1%; 4.0% to 6.3%; women 6.2
Chlamydia trachomatis Serovar Ba ATCC ® VR-347™ Designation: Apache-2 Application: Sexually Transmitted Disease Research
Greenwell, Pamela, Kakourou , Georgia and Rughooputh, Sanjiv (2006) Analysis of glycosidases activity in Chlamydia trachomatis L2 serotype. Internet Journal of Medical Update, 1 (1). ISSN 1694-0423 Full text not available from this repository ...
Chlamydia trachomatis serovars D-K are the leading cause of bacterially-acquired sexually transmitted infections in the United States. As an obligate intracellular pathogen, C. trachomatis infects columnar epithelial cells of the genital mucosae and can cause deleterious sequelae such as pelvic inflammatory disease, infertility, and ectopic pregnancy. Several chlamydial antigens reach the host cell cytosol prior to the natural release of chlamydiae at the end of the developmental cycle. While some of these extra-inclusion antigens traffic to the host cell surface, others remain intracellular where they are proposed to influence vital host cell functions and antigen trafficking and presentation. The research herein examines the escape and trafficking of the immunodominant chlamydial antigens MOMP, LPS, and cHsp60 within C. trachomatis serovar E-infected polarized human endometrial epithelial cells. Studies using high-resolution transmission electron microscopy (TEM) and immuno-TEM report the novel escape
Objective: To characterise a Chlamydio trachomatis variant strain from a patient with non-gonococcal urethritis (NGU) whose first void urine (FVU) displayed discrepant C trachomatis test results and describe the clinical response to treatment. Methods: The FVU specimen was assayed with an immune based Chlamydio Rapid Test (CRT) and various nucleic acid amplification tests (NAATs) to establish C trachomatis infection. Sequencing of the major outer membrane protein gene (omp I also known as ompA) was undertaken to identify the serovar of the variant strain. Polymerase chain reaction (PCR) analysis was also conducted to determine whether the strain harboured deletions in the cryptic plasmid or was plasmid free. Results: The FVU specimen was strongly reactive in CRT but negative with the plasmid based Amplicor PCR (Roche) and ProbeTec ET (Becton-Dickinson) assays. However, NAATs for 16S RNA (Aptima Combo 2, GenProbe), omp 1 (RealArt CT PCR, Artus and in-house NAATs) or the outer membrane complex B ...
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease for this organism. Identification and application of a unique CtHtrA inhibitor (JO146) to cultures of Chlamydia resulted in a complete loss of viable elementary body formation. JO146 treatment during the replicative phase of development resulted in a loss of Chlamydia cell morphology, diminishing inclusion size, and ultimate loss of inclusions from the host cells. This completely prevented the formation of viable Chlamydia elementary bodies. In addition to its effect on the human Chlamydia trachomatis strain, JO146 inhibited the viability of the mouse strain, Chlamydia muridarum, both in vitro and in vivo. Thus, we report a chemical biology approach to establish an essential role for
Goat polyclonal antibody raised against Chlamydia trachomatis MOMP. Native purified MOMP from Chlamydia trachomatis strain L2. (PAB13907) - Products - Abnova
The natural course of sexually transmitted infections caused by Chlamydia trachomatis varies between individuals. In addition to parasite and host effects, the vaginal microbiota might play a key role in the outcome of C. trachomatis infections. Interferon-gamma (IFN-γ), known for its anti-chlamydial properties, activates the expression of indoleamine 2,3-dioxygenase (IDO1) in epithelial cells, an enzyme that catabolizes the amino acid L- tryptophan into N-formylkynurenine, depleting the host cells pool of tryptophan. Although C. trachomatis is a tryptophan auxotroph, urogenital strains (but not ocular strains) have been shown in vitro to have the ability to produce tryptophan from indole using the tryptophan synthase (trpBA) gene. It has been suggested that indole producing bacteria from the vaginal microbiota could influence the outcome of Chlamydia infection. We used two in vitro models (treatment with IFN-γ or direct limitation of tryptophan), to study the effects of direct rescue by the addition
The aim of this study was to evaluate how patients with Chlamydia trachomatis infection perceived the legal enforcement of partner notification and to seek their views on legislation impinging on their own sexual behaviour. The investigation was performed at STD clinics in Stockholm, Sweden in 1997. Consecutive patients (n=192) answered a questionnaire about sexual behaviour and contact tracing. More men (40%) than women (21%) had had sexual intercourse during the past 6 months with an occasional partner. The mean number (6 months prior to this) was 2.3 partners (1-15) for men and 2.2 partners (1-21) for women. Eighteen per cent admitted to having avoided disclosing the name of their partner(s). Ninety per cent considered it beneficial that chlamydial infection was regulated and that a named partner could be forced to undergo STD testing. Partly based on this report, the government has recommended police enforcement to be removed from the legislation as a tool for contact tracing in chlamydial ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The results of this study indicate that consistent condom use is significantly associated with a 90% reduction in the prevalence of C trachomatis infection among those with known exposure to an infected partner. The present analyses provide a more refined estimate than previously reported by restricting the analyses to a single infection, C trachomatis, and by restricting the sample population to only those who reported exposure to infection. Indeed, our estimate exceeds others (90% effective v 26% to 58% effective), perhaps because our estimate was undiluted by the inclusion of people who do not contribute meaningful observations to the analyses-namely, individuals who may not have been exposed to C trachomatis; their condom use would not be expected to provide protection. While our estimate is greater than the one estimated by Warner and colleagues who also only included contacts to infected people, the substantial overlap of the 95% confidence intervals indicates they are indeed quite ...
Chlamydia trachomatis infection and sexual behaviour among female students attending higher education in the Republic of Ireland. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Compensatory T cell responses in IRG-deficient mice prevent sustained Chlamydia trachomatis infections.s profile, publications, research topics, and co-authors
Assessing a Potential Role of Host Pannexin 1 during Chlamydia trachomatis Infection. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
TY - JOUR. T1 - Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. AU - Horner, P. J.. AU - Ali, M.. AU - Parker, D.. AU - Weber, J. N.. AU - Taylor-Robinson, D.. AU - McClure, M. O.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - Aims - To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. Methods - The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a β-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against β-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm. Results - A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C ...
TRIVITRON HEALTHCARE PVT. LTD. - Exporter, Manufacturer, Distributor & Supplier of Chlamydia trachomatis EIA tests chlamydia-trachomatis-eia-tests chlamydia-trachomatis-eia-tests based in New Delhi, India
Quality Chlamydia Trachomatis IgM ELISA kit from ELISA kits manufacturer and elisa kits supplier: Chlamydia Trachomatis IgA ELISA kit. Our kits are FDA-CE and ISO certified.
RapidSTDTestKit Rapid Chlamydia Test [Chlamydia-T] - home chlamydia test kit Chlamidia Home Testing Kit Uses a cervical swab for women and a urethral swab for men. Rapid test to detect the bacteria Chlamydia trachomatis in males and females Easy to use - 2 easy steps Visible result in 10 minutes Test interpretation: p { margin-bottom: 0.08in; } Technical details of the test: Uses double sandwich immunoassay technology to detect
A cross-sectional survey of 1216 newly pregnant women (mean age = 31 years) from 32 general practices and five family planning clinics was conducted to find the prevalence of chlamydial infection and to evaluate self-administered vaginal swabs and first-pass urines for detection of Chlamydia trachomatis by ligase chain reaction assay. Overall prevalence of infection was 2.4% (95% CI = 1.5 to 3.3) but in women aged less than 25 years it was 8.6% (95% CI = 4.1 to 12.9) and in pregnant teenagers it was 14.3% (95% CI = 3.7 to 24.9). In 1161 women with both swab and urine results, 25 women were positive on both specimens three on swab alone, and one on urine alone. When asked which they preferred to provide, 47% said urine, 59 swab and 48% preferred both equally. This is the first study to show that non-invasive screening in early pregnancy is feasible in the community. Although swabs detected 10% more infections, nearly half the women preferred providing urine specimens. ...
Chlamydia trachomatis antibody LS-C524917 is a biotin-conjugated mouse monoclonal antibody to chlamydia trachomatis Chlamydia trachomatis. Validated for ELISA.
The etiological diagnosis of what is today known as infection by Chlamydia trachomatis was first made possible in 1907 when Halberstaedter and von Prowazek identified inclusions in conjunctival scrapings by means of Giemsa staining. C. trachomatis wa
Chlamydia infection (from the Greek, χλαμύδα meaning cloak) is a common sexually transmitted infection in humans caused by the bacterium Chlamydia trachomatis. The term Chlamydia infection can also refer to infection caused by any species belonging to the bacterial family Chlamydiaceae. C. trachomatis is found only in humans. Chlamydia is a major infectious cause of human genital and eye disease. Chlamydia infection is one of the most common sexually transmitted infections worldwide; it is estimated that about 1 million individuals in the United States are infected with chlamydia. ...
Cell division in Chlamydiae is poorly understood as apparent homologs to most conserved bacterial cell division proteins are lacking and presence of elongation (rod shape) associated proteins indicate non-canonical mechanisms may be employed. The rod-shape determining protein MreB has been proposed as playing a unique role in chlamydial cell division. In other organisms, MreB is part of an elongation complex that requires RodZ for proper function. A recent study reported that the protein encoded by ORF CT009 interacts with MreB despite low sequence similarity to RodZ. The studies in this paper expand on those observations through protein structure, mutagenesis and cellular localization analyses. Structural analysis indicated that CT009 shares high level of structural similarity to RodZ, revealing the conserved orientation of two residues critical for MreB interaction. Substitutions eliminated MreB protein interaction and partial complementation provided by CT009 in RodZ deficient Escherichia ...
Urogenital chlamydia infection, caused by the bacterium Chlamydia trachomatis (CT), is the most common sexually transmitted bacterial infection in Sweden. In 2008 it was estimated by WHO that there were 105.7 million new cases of CT worldwide, an increase by 4.2 million cases (4.1%) compared to 2005. If untreated, CT infections can progress to serious reproductive health problems, especially in women. These complications include subfertility/infertility, ectopic pregnancy and chronic pain. The CT infection is often asymptomatic and reliable diagnostic methods and contact tracing are important tools for identifying infected individuals. CT infection is classified in the Swedish Communicable Diseases Act as a serious disease; consequently, written reporting and contact tracing are compulsory. Previous or ongoing CT infection is not uncommon in infertile couples, especially in women with tubal factor infertility (TFI). We have tested 244 infertile couples for CT antibodies, and CT IgG positive ...
Chlamydia trachomatis continues to be the most commonly reported sexually transmitted bacterial infec-tion in many countries with more than 100 million new cases estimated annually. These acute infectionstranslate into significant downstream health care costs, particularly for women, where complicationscan include pelvic inflammatory disease and other disease sequelae such as tubal factor infertility.Despite years of research, the immunological mechanisms responsible for protective immunity versusimmunopathology are still not well understood, although it is widely accepted that T cell driven IFN-gand Th17 responses are critical for clearing infection. While antibodies are able to neutralize infectionsin vitro, alone they are not protective, indicating that any successful vaccine will need to elicit botharms of the immune response. In recent years, there has been an expansion in the number and types ofantigens that have been evaluated as vaccines, and combined with the new array of mucosal adjuvants,this
Mucosal immunity to Chlamydia trachomatis in a mouse model of female genital tract infection is mediated predominantly by Th1-type cells, as shown by in vivo neutralization of cytokines involved in the Th1 vs Th2 pathways. Neutralization of IL-12 was associated with an apparent decrease in the infiltration of CD4+ T cells into infected tissues, systemic reductions in the production of IFN-gamma, and prolonged shedding of high levels of bacteria. Neutralization of IL-4 had no detectable effect on host immunity or on bacterial clearance. To dissociate the protective role of IL-12 from that of IL-12-induced IFN-gamma, resistance to C. trachomatis was compared in IL-12-depleted and IFN-gamma-deficient animals. IL-12-depleted mice displayed minimal bacterial clearance for 1 mo post-infection but eventually resolved genital tract infections completely. IFN-gamma-deficient mice, on the other hand, cleared 99.9% of genital Chlamydia within the first 3 wk but then developed systemic disease associated ...
Mucosal immunity to Chlamydia trachomatis in a mouse model of female genital tract infection is mediated predominantly by Th1-type cells, as shown by in vivo neutralization of cytokines involved in the Th1 vs Th2 pathways. Neutralization of IL-12 was associated with an apparent decrease in the infiltration of CD4+ T cells into infected tissues, systemic reductions in the production of IFN-gamma, and prolonged shedding of high levels of bacteria. Neutralization of IL-4 had no detectable effect on host immunity or on bacterial clearance. To dissociate the protective role of IL-12 from that of IL-12-induced IFN-gamma, resistance to C. trachomatis was compared in IL-12-depleted and IFN-gamma-deficient animals. IL-12-depleted mice displayed minimal bacterial clearance for 1 mo post-infection but eventually resolved genital tract infections completely. IFN-gamma-deficient mice, on the other hand, cleared 99.9% of genital Chlamydia within the first 3 wk but then developed systemic disease associated ...
Novel simultaneous RNA-Seq analysis tracks host/pathogen interactions. Investigators at the Institute for Genome Sciences at the University of Maryland School of Medicine have developed a new technique that can track the activity of a disease-causing microbe and the host cell response to that pathogen simultaneously. Using the new method to examine Chlamydia trachomatis infection, the study team observed how the response of the infected cell contributes to one of the hallmark outcomes of chlamydial disease -- tissue scarring. Their findings appear in the December 4 issue of PLOS One.. Chlamydia trachomatis is an intracellular, disease-causing bacterium responsible for the most common human sexually transmitted infections (STIs) and infectious blindness (trachoma) globally. Sexually transmitted chlamydial infections are often asymptomatic, and cause tissue damage and scarring. For example, chlamydial-induced scar tissue within the fallopian tubes can block the tubal opening and lead to ...
Development of sensitive and reproducible assays for clinical and life science research of C. trachomatis is essential to monitor and help prevent further spread of this pathogen. DNA was purified from 130 urine research samples using a fully automated protocol on the BioRobot® M48 workstation together with the MagAttract® DNA Mini M48 Kit. Purified DNA performed well in a real-time PCR assay being developed for the amplification of Chlamydia trachomatis DNA. Comparison of this easy-to-use proce
Introduction: Parvovirus B19, Toxoplasma gondii and Chlamydia trachomatis are the most common microorganisms which cause different sequela during pregnancy such as c...