TY - JOUR. T1 - Expression studies of Bacillus licheniformis chitin deacetylase in E. coli Rosetta cells. AU - Raval, Ritu. AU - Simsa, Robin. AU - Raval, Keyur. PY - 2017/11/1. Y1 - 2017/11/1. N2 - Chitin, the biopolymer of the N-acetylglucosamine, is the most abundant biopolymer on the planet after cellulose. However owing to its crystalline nature, its deacetylated derivative; chitosan is industrially more potent. This conversion on an enzymatic scale can be made using chitin deacetylase. The metagenomics library constructed from the soil exposed to chitin and chitosan yielded chitin modifying enzymes, one of them being chitin deacetylase (CDA) utilized for the present study. The gene was amplified and expressed using the pET 22b vector in E. coli Rosetta cells. The effect of two additives; chitin and glycerol on the CDA activity were studied. The inclusion of glycerol in the medium improved the biomass by 50% from the initial value of 1.25 g/l to 2.5 g/l. The activity of CDA increased from ...
This book contains comprehensive contributions on chitin biology and chitin metabolism related enzymes, aiming to provide with recent research progress about biology of chitin-containing organisms as well as cross-talks between chitin-containing and non-chitin-containing organisms.
The 1011 ton global annual turnover of chitin has generated extensive interest in the regulation of chitin processing enzyme production in bacteria. Some bacteria regulate chitinase production by N-Acyl-L-homoserine lactone (AHL) mediated quorum sensing. In this study, a description of bacterial community succession during chitin particle colonisation and depolymerisation in activated sludge is presented. It was discovered that Betaproteobacteria and Bacteroidetes lineages dominate chitin colonisation in sludge and that AHLs bind to chitin at concentrations that upregulate AHL dependent transcription in bacterial cells associated with the chitin surface. There was no requirement for high cell density (a quorum) at the chitin surface. Further, N-Acetyl glucosamine (GlcNAc), the monomer of the chitin polymer, is shown to inhibit AHL dependent gene transcription representing a previously unrecognised mechanism by which the chitinase reaction product negatively regulates chitinase production. ...
Chitin and its derivatives-as a potential resource as well as multiple functional substrates-have generated attractive interest in various fields such as biomedical, pharmaceutical, food and environmental industries, since the first isolation of chitin in 1811. Moreover, chitosan and its chitooligosaccharides (COS) are degraded products of chitin through enzymatic and acidic hydrolysis processes; and COS, in particular, is well suited for potential biological application, due to the biocompatibility and nontoxic nature of chitosan. In this review, we investigate the current bioactivities of chitin derivatives, which are all correlated with their biomedical properties. Several new and cutting edge insights here may provide a molecular basis for the mechanism of chitin, and hence may aid its use for medical and pharmaceutical applications.
Chitin is abundant in nature due to its compact intractable and inert structure resulted from strong hydrogen bonding network. Chitin is known as one of the second most abundant polysaccharides in nature, after cellulose. In crustaceans, chitin is present in a complex structure with calcium carbonate, forming the rigid skeleton of carapace, shell and tail. In insects, chitin is the main building block of the back plate. This intractable characteristic of chitin is superior in the animal / plant kingdom as protective skeleton but is a major disadvantage for chemical / physical modification. Therefore more efficient methods of reacting or modifying chitin (especially alpha-chitin as it is the most abundant of the 3 types of naturally occurring chitin) is necessary, in order to utilize this biomass as a major renewable raw materials ...
Molecular Plant-Microbe Interactions 15:963-970...Bing Zhang , 1 Katrina Ramonell , 2 Shauna Somerville , 2 and Gary Stacey 1 , 3...© 2002 The American Phytopathological Society...Three genes (i.e., a zinc finger protein, a lectin-like protein, and AtMPK3), previously shown to respond to chitin elicitation in microarray experiments, were used to examine the response of Arabidopsis spp. to chitin addition. Maximum induction for all three genes was found upon addition of crab-...
Vibrio cholera survival in an aquatic environment depends on chitin utilization pathway that requires two factors, chitin binding protein and chitinases. The chitinases and the chitin utilization pathway are regulated by a two-component sensor histidine kinase ChiS in V. cholerae. In recent studies these two factors are also shown to be involved in V. cholerae pathogenesis. However, the role played by their upstream regulator ChiS in pathogenesis is yet to be known. In this study, we investigated the activation of ChiS in presence of mucin and its functional role in pathogenesis. We found ChiS is activated in mucin supplemented media. The isogenic chiS mutant (ChiS-) showed less growth compared to the wild type strain (ChiS+) in the presence of mucin supplemented media. The ChiS- strain also showed highly retarded motility as well as mucin layer penetration in vitro. Our result also showed that ChiS was important for adherence and survival in HT-29 cell. These observations indicate that ChiS is ...
Vibrio cholera survival in an aquatic environment depends on chitin utilization pathway that requires two factors, chitin binding protein and chitinases. The chitinases and the chitin utilization pathway are regulated by a two-component sensor histidine kinase ChiS in V. cholerae. In recent studies these two factors are also shown to be involved in V. cholerae pathogenesis. However, the role played by their upstream regulator ChiS in pathogenesis is yet to be known. In this study, we investigated the activation of ChiS in presence of mucin and its functional role in pathogenesis. We found ChiS is activated in mucin supplemented media. The isogenic chiS mutant (ChiS-) showed less growth compared to the wild type strain (ChiS+) in the presence of mucin supplemented media. The ChiS- strain also showed highly retarded motility as well as mucin layer penetration in vitro. Our result also showed that ChiS was important for adherence and survival in HT-29 cell. These observations indicate that ChiS is ...
小菜蛾 (Plutella xylostella, diamondback),繁殖能力強,生長世代短,可適應各種惡劣的環境,為世界性的十字花科作物重要害蟲,然而其幾丁質相關構造及酵素目前仍未被詳細研究。幾丁質為構成昆蟲表皮及圍食膜 (peritrophic matrix: PM) 之重要物質;幾丁質通常會與幾丁質結合蛋白 (chitin binding proteins: CBPs) 結合,CBPs對於幾丁質重要構造的形成、結構維持以及功能的調控,扮演重要的角色。本研究解析小菜蛾體內幾丁質重要構造之蛋白與幾丁質相關酵素,特別針對圍食膜蛋白 (PM proteins: PMP)、CBPs以及幾丁質? (Chitinase: Chts) 等幾丁質相關蛋白,根據CBPs之基因表現情形、親緣關係分析及幾丁質?選殖與表現定性,期望獲得有助於研發新式鱗翅目害蟲防治之重要資訊。利用2-D電泳與質譜分析,可鑑定到四個 PM ...
Chitin (C8H13O5N)n (/ˈkaɪtɪn/ KY-tin), a long-chain polymer of N-acetylglucosamine, is a derivative of glucose. It is a primary component of cell walls in fungi, the exoskeletons of arthropods, such as crustaceans (e.g., crabs, lobsters and shrimps) and insects, the radulae of molluscs, cephalopod beaks, and the scales of fish and lissamphibians. The structure of chitin is comparable to another polysaccharide - cellulose, forming crystalline nanofibrils or whiskers. In terms of function, it may be compared to the protein keratin. Chitin has proved useful for several medicinal, industrial and biotechnological purposes. The English word "chitin" comes from the French word chitine, which was derived in 1821 from the Greek word χιτών (chiton), meaning covering. A similar word, "chiton", refers to a marine animal with a protective shell. The structure of chitin was determined by Albert Hofmann in 1929. Chitin is a modified polysaccharide that contains nitrogen; it is synthesized from units of ...
Cellulose nanofibers and chitin nanocrystals, two main components of agricultural and aquacultural by-products, were obtained from blue agave and yellow squat lobster industrial residues. Cellulose nanofibers were obtained using high pressure homogenization, while chitin nanocrystals were obtained by hydrolysis in acid medium. Cellulose nanofibers and chitin nanocrystals were characterized by X-ray diffraction, Atomic Force Microscopy and Infrared spectroscopy. Self-bonded composite films with different composition were fabricated by hot pressing and their properties were evaluated. Antifungal activity of chitin nanocrystals was studied using a Cellometer®cell count device, mechanical properties at tension were measured with a universal testing machine, water vapor permeability was evaluated with a thermohygrometer and surface tension with sessile drop contact angle method. The addition of chitin nanocrystals reduced slightly the mechanical properties of the composite. Presence of chitin ...
Chitin concentrations greater than 0.04% (wt/wt) protected cholera vibrios against killing at low temperature. This protective effect was detected with both the soluble form of chitin, glycol chitin, and the insoluble particulate form of chitin. Some amino acids or peptides also showed the same protective effect. ...
Chitin concentrations greater than 0.04% (wt/wt) protected cholera vibrios against killing at low temperature. This protective effect was detected with both the soluble form of chitin, glycol chitin, and the insoluble particulate form of chitin. Some amino acids or peptides also showed the same protective effect. ...
Chitin binding appeared to be specific and the authors presented some data that suggested that some of ChtVis-Tomato was stably bound to chitin while some seemed to be continually binding and releasing from chitin.. ChtVis-Tomato was useful for making long time lapse observations in vivo.. The ChtVis-Tomato reporter was construted in the pWALIUM10-moe vector and inserted into the Drosophila genome using phiC31 mediated integration. ChtVis-Tomato is under the regulatory control of a 5XUAS-containing promoter allowing it to be expressed in any Gal4 driver lines of D. melanogaster. The reporter is flanked by gypsy insulator sequences. pWALIUM10-moe contains a mini white gene that serves as a genetic marker in the appropriate mutant white background.. With a bit of modification and reconfiguring ChtVis-Tomato could easily be deployed in any insect and is expected to function as it has in D. melanogaster.. Lukasz F. Sobala, Ying Wang, Paul N. Adler (2015) ChtVis-Tomato, a genetic reporter for in vivo ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Journal of Chemistry is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles on all aspects of fundamental and applied chemistry.
The breakdown of chitin within an acidic upland grassland was studied. The aim was to provide a molecular characterisation of microorganisms involved in chitin degradation in the soil using soil micro
Im having troubles finding a description or picture of how chitin molecules bind together to form the exoskeletons of arthropods, crustaceans, etc. I have found a couple of cool pictures of the chitin molecule itself, but no images of how they link. My biology book has a long description of how glucose forms either cellulose or starch depending on if it is an alpha or beta configuration, and how they link up to form 3D structures. But it just shows a picture of the chitin molecule and completely blows of any 3D structure description. It is not really for any purpose, Im just very curious. Thanks for your help in advance. BK ...
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1. Slámová K, Bojarová P, Petrásková L. et al. β-N-Acetylhexosaminidase: Whats in a name…?. Biotechnol Adv. 2010;28:682-93 2. Merzendorfer H, Zimoch L. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol. 2003;206:4393-412 3. Nagamatsu Y, Yanagisawa I, Kimoto M. et al. Purification of a chitooligosaccharidolytic β-N-acetylglucosaminidase from Bombyx mori larvae during metamorphosis and the nucleotide sequence of its cDNA. Biosci Biotechnol Biochem. 1995;59:219-25 4. Yang Q, Liu T, Liu F. et al. A novel β-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenée). FEBS J. 2008;275:5690-702 5. Liu T, Zhang H, Liu F. et al. Structural determinants of an insect β-N-acetyl-D-hexosaminidase specialized as a chitinolytic enzyme. J Biol Chem. 2011;286:4049-58 6. Zheng YP, Krell PJ, Doucet D. et al. Cloning, expression, and localization of a molt-related β-N-acetylglucosaminidase in the Spruce budworm, Choristoneura ...
Chitooligosaccharides (COS) with degrees of polymerization (DP) 6 to 8 are degraded from chitosan, which possess excellent bioactivities. However, technologies that could purify them from hydrolysis mixtures in the narrow DP range (984 to 1,306 Da) are absent. The objective of this research is to purify DP 6 to 8 COS by nanofiltration on the basis of appropriate adjustments of the feed condition. Syrup containing DP 6 to 8 COS at different concentrations (19.0 to 46.7 g/L) was prepared. A commercial membrane (QY-5-NF-1812) negatively charged was applied. Experiments were carried out in full recycle mode, so that the observed COS retentions were investigated at various transmembrane pressures (6.0 to 20.0 bar), temperatures (10°C to 50°C), and pHs (5.0 to 9.0). Then, the feasibility of separation of DP 6 to 8 COS was further studied by concentration ratio under optimum conditions. The results indicate that the purification of DP 6 to 8 COS by nanofiltration NF is feasible. It was found that the
SCIN - Self-regenerating Chitin INduction Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications. ...
SCIN - Self-regenerating Chitin INduction Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications. ...
Cuttlefish are mollusks that look somewhat like squid. They have an internal, hard, supportive structure with soft organs around and inside it. This resilient cuttlebone is made of cleverly woven strands of a biochemical material called chitin and mixed with a hard biomineral called aragonite.. A team of paleontologists found a supposedly 34 million-year-old fossil cuttlebone that still had both the original aragonite and chitin. This is significant, because one might expect the hard aragonite to persist in the fossil record, but not the organic chitin or protein. The chitin, which is made of sugars tightly bonded into molecular chains, would have spontaneously degraded and been long gone after only thousands of years.. The researchers compared the fossil cuttlebone, found in a Mississippi clay deposit, with modern cuttlebone chitin, and their results showed a shortening of the chitin strands,…the breakdown of chitosan [chemically altered chitin],… and loss of hydrogen bonds.1 Thus, the ...
The predominant characteristic feature of C. pinensis is the ability to degrade chitin, a β-1,4-glycosidic linked homopolymer of N-acetyl-D-glucosamine and one of the most abundant polysaccharides in nature. It is a component of fungal cell walls and of arthropod exoskeletons. Chitin is degraded by chitinases (EC 3.2.1.14); endochitinases randomly cleave within the chitin molecule and exochitinases hydrolyze diacetylchitobiose from the end of a chitin chain. Diacetylchitobiose is further degraded to N-acetylglucosamine by the action of N-acetylglucosaminidases (EC 3.2.1.52).. These glycosidic bond hydrolyzing enzymes were grouped in glycoside hydrolase (GH) families based on amino acid sequence similarities (Web Site) [22]. For the C. pinensis genome 169 glycoside hydrolases belonging to 49 different GH families are predicted; 18 of the predicted glycoside hydrolases belong to GH family 43 which contains xylosidases, xylanases, arabinanases, arabinofuranosidases and galactosidases. Because of ...
chitin is a fascinating polysaccharide for a number of reasons. First, it is the second most abundant polycarbohydrate used by Mother Nature as a structural component both in the animal and in the plant kingdoms. Second, chitin has a chemical structure which is similar to that of cellulose
Chitin, a polymer of N-acetylglucosamine, is an important structural component in the cell walls of fungal pathogens. Plant chitinases are capable of degrading this component to directly inhib...
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New research in mice by UC San Francisco scientists shows that the lungs secrete a specialized enzyme capable of destroying chitin, without which chitin particles inhaled from the environment can accumulate in the airways and trigger inflammatory lung disease.
Modules of approx. 70 residues. The chitin-binding function has been demonstrated in several cases. These modules are found attached to a number of chitinase catalytic domains, but also in non-catalytic proteins either in isolation or as multiple repeats; chitin binding (EC IIa.chitin ...
Silkworm pupae meal is a protein-rich feed ingredient with a high nutritional value. Its crude protein content ranges from 50% DM to more than 80% DM (for defatted meal). The lysine (6-7% of the protein) and methionine (2-3% of the protein) contents are particularly high. However, the true protein (calculated as the sum of amino acids) in silkworms was found to correspond to only 73% of the crude protein content (Finke, 2002), which was explained by the presence of chitin, since this component contains nitrogen. However, the chitin content of pupae meal is relatively low, about 3-4% DM (Finke, 2002; Suresh et al., 2012). The presence of chitin and insoluble protein may also explain the presence of fibre, and values of 6-12% DM of ADF have been reported (Finke, 2002; Ioselevich et al., 2004). Undefatted pupae meal is rich in fat, typically in the 20-40% DM range. Defatted meal contains less than 10% oil in the DM. Silkworm oil contains a high percentage of polyunsaturated fatty acids, notably ...
Literatura Citada. Barber, M. S., R. E. Bertram, and J. P. Ride. 1989. Chitin oligosaccharides elicit lignification in wounded wheat leaves. Physiol. Mol. Plant Pathol. 34: 3-12. [ Links ] Barros, J., H. Serk, I. Granlund, and E. Pesquet. 2015. The cell biology of lignification in higher plants. Ann. Bot. 115: 1053-1074. [ Links ] Brugnerotto, J., J. Lizardi, F. M. Goycoolea, W. Argüelles-Monal, J. Desbrieres, and M. Rinaudo. 2001. An infrared investigation in relation with chitin and chitosan characterization. Polymer. 42: 3569-3580. [ Links ] Cabrera, J. C., and P. Van Cutsem. 2005. Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan. Biochem. Eng. J. 25: 165-172. [ Links ] Catinot, J., A. Buchala, E. Abou-Mansour, and J. P. Metraux. 2008. Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana. FEBS Lett. 582: 473-478. [ Links ] Chen, Z., Z. Zheng, J. Huang, ...
Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG
Lysin motif (LysM) receptor kinases are unique to plants and serve important functions in plant-microbe interactions. These proteins recognize microbe-derived N-acetylglucosamine (NAG)-containing ligands, but the molecular mode of ligand perception and of receptor activation has remained unknown. The three-dimensional structure of the LysM receptor kinase CERK1 (chitin elicitor receptor kinase 1) from Arabidopsis thaliana has been reported. CERK1 binds NAG oligomers derived from chitin-the major constituent of fungal cell walls-and mediates immunity to fungal infection. The crystal structure of CERK1 complexed with a NAG pentamer revealed that three NAG moieties attach tightly to one of three lysin motifs within the CERK1 ectodomain. Receptor activation and immune signaling requires, however, ligand-induced CERK1 homodimerization. By acting as bivalent ligands, NAG octamers stabilize CERK1 dimers, providing a structural explanation for why the immunogenic activity of NAG oligomers is restricted ...
Marshall Marine Products is an Indian Marine Biotechnology Company, focused on Chitin and its derivatives. We pioneered in the development of Chitin Science, working relentlessly on improving the production technologies, and exploring new opportunities for developing new and innovative applications, by exploiting the unique properties of Chitin and its Derivatives. We coordinate, communicate, and collaborate with Industrial, and Scientific Research & Development and with Academic Communities
Lufenuron is much better known in the veterinary world as Program. It is used to treat parasite infections such as fleas. It works because it inhibits chitin production. Humans and, indeed, all mammals do not produce chitin and therefore the potential for side effects in humans is very small. Insects do need chitin to make a tough exoskeleton. Interestingly, so do yeasts and moulds - it is chitin in their cell wall that makes them so tough and difficult to kill. Therefore, potentially Lufenuron can be used to kill yeasts and moulds and, indeed, this has been well demonstrated in the veterinary world. It is not metabolised or eliminated by the liver or kidneys, but excreted through the faeces and again this makes it remarkably non-toxic. Lufenuron is very lipid soluble - this means it is taken over five days and loads up into fatty tissues from which it is slowly released over four to six weeks. This maintains tissue concentrations high enough to kill all yeast, including candida, for at least a ...
One has a pink/brown hue, the other a steely blue hue. Two full top shells, and four full bottom shell halves. Forged by Greycore there is a Chitin Shield and
My first wallpiece of the year was in our traditional style, assembled from folded boxes, but I wanted to make another with my newer design style and construction technique, which I refer to as chitin, like wallpiece RCB 11.04, which recently sold at our show at Manna Gallery. So I began this piece as soon as the previous one was well under way, and finished it yesterday. ...
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Complete information for FIBCD1 gene (Protein Coding), Fibrinogen C Domain Containing 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Today I would like to introduce my new Kill Team: The Zoats. Not much is known these days about this xenos race. I focused on the part of their lore regarding their supposed enslavement by the Tyranids. The question now is, how the Tyranids would be able to do so. Thus I came up with a whole new-made up unit, the Cephalotrophs. The Cephalotrophs are a small, floating breed of Tyranids who have quite a fragile body which mainly is a gland plus a little tail. While on the hunt their chitin scale is used to protect them but as soon as they find a possible host they try to hug them from behind. Flesh hooks are fired .... ...
These creatures have developed a chitin armor around its fangs, legs and abdomen. Source: Pet Battle: Suramar. Beast battle pet. This NPC is the criteria of Broken Isles Safari.
Modules of approx. 60 residues found in bacterial enzymes. Chitin-binding described in several cases. Distantly related to the CBM12 family ...
Chitin deacetylase, the enzyme that catalyzes the hydrolysis of acetamido groups of N-acetylglucosamine in chitin, has been purified to homogeneity from mycelial extracts of the fungus Mucor rouxii and further characterized. The enzyme exhibits a low pI (approximately 3). Its apparent molecular mass was determined to be approximately 75 kDa by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and approximately 80 kDa by size-exclusion chromatography, suggesting that the enzyme exists as a monomer. Carbohydrate analysis of purified chitin deacetylase revealed that the enzyme is a high-mannose glycoprotein and that its carbohydrate content is approximately 30% by weight. Chitin deacetylase is active on several chitinous substrates and chitin derivatives. The enzyme requires at least four N-acetylglucosamine residues (chitotetraose) for catalysis, and it is inhibited by carboxylic acids, particularly acetic acid. When glycol chitin (a water-soluble chitin derivative) was used as substrate, ...
TY - JOUR. T1 - Analysis of chitin particle size on maximum power generation, power longevity, and Coulombic efficiency in solid-substrate microbial fuel cells. AU - Rezaei, Farzaneh. AU - Richard, Tom L.. AU - Logan, Bruce E.. PY - 2009/7/15. Y1 - 2009/7/15. N2 - Microbial fuel cells (MFCs) produce bioelectricity from a wide variety of organic and inorganic substrates. Chitin can be used as a slowly degrading substrate in MFCs and thus as a long-term fuel to sustain power by these devices in remote locations. However, little is known about the effects of particle size on power density and length of the power cycle (longevity). We therefore examined power generation from chitin particles sieved to produce three average particle sizes (0.28, 0.46 and 0.78 mm). The longevity increased from 9 to 33 days with an increase in the particle diameter from 0.28 to 0.78 mm. Coulombic efficiency also increased with particle size from 18% to 56%. The maximum power density was lower for the largest (0.78 mm) ...
Summary: A fraction which inhibited chitin synthesis was partially purified from Neurospora crassa by ammonium sulphate precipitation and gel filtration. This preparation possessed chitinase activity and hydrolysed either nascent or preformed chitin. Utilization of UDP-N-acetylglucos-arnine by chitin synthase was not modified in the presence of the chitinase preparation, although the chitin being synthesized was degraded mainly to N-N'-diacetylchitobiose, other larger oligosaccharides and small amounts of N-acetylglucosamine. The enzyme exhibited endo- and exo-chitinase properties and was localized mainly in the cytosol fraction. Its pH optimum was 6.7 and its apparent molecular weight 20600 Dal.
A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation.
Chitin Oligosaccharide COS Reduces Antibiotics Dose and Prevents Antibiotics-Caused Side Effects in Adolescent Idiopathic Scoliosis AIS Patients with Spinal Fusion Surgery. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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