The European Centre for Disease Prevention and Control issued a risk assessment on the ongoing outbreak of chikungunya virus infection in Italy. Two r...
The Mississippi State Department of Health has confirmed a number of cases of chikungunya virus infection in Mississippi residents recently returned from Haiti and the Dominican Republic. Other possible cases are under investigation. Mississippians who travel to the Dominican Republic, Haiti, Puerto Rico or other Caribbean countries are at risk of chikungunya virus. Chikungunya can spread from person to person through the bite of a mosquito once travelers return home. See protective measures for travelers ». ...
Chikungunya fever is a pandemic disease caused by the mosquito-borne Chikungunya virus (CHIKV). E1 glycoprotein mediation of viral membrane fusion during CHIKV infection is a crucial step in the release of viral genome into the host cytoplasm for replication. How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored. A bicistronic baculovirus expression system to produce recombinant baculoviruses for cell-based assay was used. Sf21 insect cells infected by recombinant baculoviruses bearing wild type or single-amino-acid substitution of CHIKV E1 and EGFP (enhanced green fluorescence protein) were employed to investigate the roles of four E1 amino acid residues (G91, V178, A226, and H230) in membrane fusion activity. Western blot analysis revealed that the E1 expression level and surface features in wild type and mutant substituted cells were similar. However, cell fusion assay found that those cells infected by
Chikungunya fever is a pandemic disease caused by the mosquito-borne Chikungunya virus (CHIKV). E1 glycoprotein mediation of viral membrane fusion during CHIKV infection is a crucial step in the release of viral genome into the host cytoplasm for replication. How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored. A bicistronic baculovirus expression system to produce recombinant baculoviruses for cell-based assay was used. Sf21 insect cells infected by recombinant baculoviruses bearing wild type or single-amino-acid substitution of CHIKV E1 and EGFP (enhanced green fluorescence protein) were employed to investigate the roles of four E1 amino acid residues (G91, V178, A226, and H230) in membrane fusion activity. Western blot analysis revealed that the E1 expression level and surface features in wild type and mutant substituted cells were similar. However, cell fusion assay found that those cells infected by
Inthukantham Kashaya and Vilvadi Gulika are the medicines that can be taken as preventive measures of chikungunya .Ayurveda suggests carrot and sunflower seeds as good supplements for chikungunya patients as they strengthen persons resistance to fight disease. Triphala, an ayurvedic composition of Harada, Amla and Behada and dry seedless grapes are also effective in controlling symptoms of chikungunya. Ayurvedic treatment for bringing relief from various symptoms of chikungunya include: Fever in Chikunguya: Sacred Basil (Tulsi) leaves are very effective in reducing chikungunya fever. Vilvadi Gulika, Amritarishta and Sudarsanam Gulika are the other commonly prescribed ayurvedic medicines for fighting the fever. Muscle and Joint Pain in Chikungunya The patients of chikungunya face severe joint and muscle pain. This pain can even persist from months to years. Useful ayurvedic medications for controlling pain in chikungunya include: ...
Infection with Chikungunya alphavirus (CHIKV) can cause severe arthralgia and chronic arthritis in humans with persistence of the virus in perivascular macrophages of the synovial membrane by mechanisms largely ill-characterized. We herein analysed the innate immune response (cytokine and programmed cell death) of RAW264.7 mouse macrophages following CHIKV infection. We found that the infection was restrained to a small percentage of cells and was not associated with a robust type I IFN innate immune response (IFN-α4 and ISG56). TNF-α, IL-6 and GM-CSF expression were upregulated while IFN-γ, IL-1α, IL-2, IL-4, IL-5, IL-10 or IL-17 expression could not be evidenced prior to and after CHIKV exposure. Although CHIKV is known to drive apoptosis in many cell types, we found no canonical signs of programmed cell death (cleaved caspase-3, -9) in infected RAW264.7 cells. These data argue for the capacity of CHIKV to infect and drive a specific innate immune response in RAW264.7 macrophage cell which seems
Another name for Chikungunya Disease is Chikungunya Disease. What is Chikungunya disease? A person with Chikungunya disease has an infection caused by ...
Author Summary Chikungunya virus (CHIKV) is transmitted by mosquito bites and causes a febrile disease that is often characterized by persistent joint pain. Until recently, CHIKV outbreaks were limited to tropical areas of Africa and Asia. However, since 2007, following a large CHIKV epidemic in the Indian Ocean and South-East Asia, CHIKV has also been reported in temperate European regions. As mosquito habitats expand, virus dissemination may become more prevalent, but there are currently no vaccines or CHIKV-specific treatments available. We previously described two human antibodies that potently block cellular CHIKV infection. In the current report, we have characterized CHIKV mutants that escape neutralization to identify sub-domains of the virus envelope which are involved in CHIKV interaction with these antibodies, thereby opening the door for the development of CHIKV-specific sub-domain vaccination strategies. For the first time, we have also demonstrated that CHIKV can be directly transmitted
In 2005-6, more than 1.5 million people in the Indian Ocean area were infected with Chikungunya fever. In Reunion Island, 38.3% of the population, approximately 300 000 people, were infected.1 Only 6% of the cases were asymptomatic. Chikungunya fever is a viral infection transmitted to humans by Aedes mosquitoes.2,3 Chikungunya infection usually causes a syndrome characterised by fever, headaches, chills, conjunctivitis, rash, myalgia and severe joint pain with or without swelling. Joint symptoms vary from polyarthralgia to symmetrical polyarthritis. The outcome is usually benign, but persistent joint pain and stiffness may occur.3,4,5,6,7,8. We report 21 cases … ...
The Cook Islands Ministry of Health has confirmed seven cases of chikungunya on Rarotonga since November 2014, in which three of the cases were considered locally acquired.. Local transmission means that mosquitoes in the area have been infected with chikungunya and are spreading it to people.. CDC recommends that travelers to the Cook Islands protect themselves from mosquito bites. Some travelers may be more likely to get chikungunya, have severe disease, or be at higher risk for other reasons. CDC advises travelers in high-risk groups to discuss their travel plans with their health care provider.. Chikungunya is a viral disease transmitted by the bite of infected mosquitoes such as Aedes aegypti and Aedes albopictus. It can cause high fever, join and muscle pain, and headache. Chikungunya does not often result in death, but the joint pain may last for months or years and may become a cause of chronic pain and disability.. There is no specific treatment for chikungunya infection, nor any ...
Their newest investigation analyzed recent events in chikungunya virus evolution that will aid in predicting future trends in transmission and circulation that determine epidemic potential. Weaver and his team found that the initial adaption provided the framework for a second wave of adaptations that can enable rapid diversification of viral strains and even more efficient transmission to people. In addition, analysis of the chikungunya virus strain expressing a combination of the second-wave adaptive mutations revealed a similar pattern of changes and heightened adaptive qualities suggesting the future emergence of even higher transmission efficiency. ...
Abstract Five species of mosquito were studied to compare their susceptibility to infection, ability to sustain infection, and vector efficiency over a 4- to 5-week period after being infected with an African strain of chikungunya virus. Aedes triseriatus and Aedes albopictus were readily infected with the virus and maintained the highest infection and transmission rates of the mosquitoes tested during the test period. Aedes togoi also maintained a very high infection rate, but was a poor transmitter. Eretmapodites chrysogaster exhibited fairly high infection rates throughout the test and relatively high transmission rates in the latter half of the 35-day test period. Surprisingly low infection and transmission rates were found for Aedes aegypti.
BioCollections Worldwide said today that it is now offering a laboratory-developed test for the detection of Chikungunya virus. The assay follows the US Centers for Disease Control and Preventions real-time RT-PCR protocol for detection of Chikungunya virus, and detects cases in the acute stage of infection in about five hours, BCW said. The Miami-based company is providing the test through its CLIA-certified testing laboratory.
...Recent breakthroughs in Chikungunya research spearheaded by scientists...Chikungunya fever caused by the Chikungunya virus (CHIKV) is a mosq...CHIKV infection is characterised by an abrupt onset of fever frequentl...To devise strategies to stop CHIKV transmission Dr Ngs team collabor...,Breakthroughs,in,Chikungunya,research,from,A*STAR,spell,new,hope,for,better,treatment,and,protection,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
MANHASSET, NY - Lindsay Lohan getting the mosquito-transmitted virus Chikungunya may help shine a light on the seriousness of the virus, according to a New York infectious disease physician.. "There is an epidemic of the virus in the Caribbean and a few cases in Florida," says Bruce Hirsch, MD, attending infectious disease physician at North Shore University Hospital in Manhasset.. Ms. Lohan contracted the virus while on vacation in French Polynesia.. "Doctors hope her celebrity status reminds us of the importance of learning and preventing emerging health concerns," Dr. Hirsch says. "The Chikungunya virus causes fever and arthritis. Older persons with the virus can have painful arthritis for long periods.". In addition to fever and arthritis, some symptoms of Chikungunya include:. ...
Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes albopictus and Aedes aegypti mosquitoes in tropical areas of Africa, Asia, and the islands of the Indian Ocean. In 2007 and 2009, CHIKV was transmitted outside these tropical areas and caused geographically localized infections in people in Italy and France. To temporally and spatially characterize CHIKV infection of Ae. albopictus midguts, a comparison of viral distribution in mosquitoes infected per os or by enema was conducted. Ae. albopictus infected with CHIKV LR 5′ green fluorescent protein (GFP) at a titer 10[superscript 6.95] tissue culture infective dose[subscript 50] (TCID[subscript 50])/mL, were collected and analyzed for virus dissemination by visualizing GFP expression and titration up to 14 days post inoculation (dpi). Additionally, midguts were dissected from the mosquitoes and imaged by fluorescence microscopy for comparison of midgut infection patterns between orally- and enema-infected mosquitoes. When virus was ...
The reemergence of Chikungunya virus exemplifies the need to develop new vaccines to target other emerging viral pathogens. Chikungunya is estimated to be responsible for several million human infections in the last decade. In addition, one of its vectors, the Aedes albopictus mosquito, is moving into new regions, meaning the infection rate is expected to…
Dengue virus (DENV) and Chikungunya virus (CHIKV) are arboviruses that share the same |i|Aedes|/i| mosquito vectors and thus overlap in their endemic areas. These two viruses also cause similar clinical presentations, especially in the initial stages of infection, with neither virus possessing any specific distinguishing clinical features. Because the outcomes and management strategies for these two viruses are vastly different, early and accurate diagnosis is imperative. Diagnosis is also important for surveillance, outbreak control, and research related to vaccine and drug development. Available diagnostic tests are aimed at detection of the virus, its antigenic components, or the host immune antibody response. In this review, we describe the recent progress and continued challenges related to the diagnosis of DENV and CHIKV infections.
Q: I heard there was a new viral disease from Africa that has just come to the Americas. What is this?A: It sounds like you are referring to the chikungunya virus.Chikungunya (which means stooped walk in a Tanzanian language, named because the joint pain from it makes people walk in a bent over fashion) is a viral illness transmitted by infected mosquitoes (only certain types of mosquitoes can carry this virus). The cycle of infection is: a mosquito bites a person (or certain animals) that
Q: I heard there is a new viral disease from Africa that has just come to the Americas. What is this?A: It sounds like you are referring to the chikungunya virus.Chikungunya (which means stooped walk in a Tanzanian language; named because the joint pain from it makes people walk in a bent-over fashion) is a viral illness transmitted by infected mosquitoes (only certain types of mosquitoes can carry this virus). The cycle of infection is: a mosquito bites a person (or certain
Florida case is unusual. Hirschwerk said he and his colleagues diagnosed chikungunya fever virus within the past six weeks. The Long Island patients were unrelated, he said.. "One of those cases has been confirmed by the CDC," Hirschwerk said, "and testing is still pending on the others.. "The likelihood of having more cases in individuals who have not traveled is very low," Hirschwerk said. "The Florida case is unusual and I dont expect to see high numbers [of infections] but we have to keep an eye on the situation.". For some experts, the emergence of chikungunya in Florida sounds an alarm because the virus is carried by mosquito species -- Aedes albopictus and Aedes aegypti-- that are well established in this country.. "The two species of Aedes mosquitoes that spread chikungunya are found in the southern and eastern United States," said Dr. Erin Staples, a medical epidemiologist at the CDC.. "It is difficult to predict how the disease will spread in the mainland U.S.," Staples said, "but we ...
Chikungunya fever is a mosquito-borne febrile illness caused by Chikungunya virus (CHIKV), an alphavirus from the Togaviridae family. It is transmitted by primarily Aedes aegytpi and Aedes albopictus mosquitos [1]. Once of little importance in the Am ...
Author Summary Dengue and chikungunya are mosquito-borne diseases that are found in similar geographical areas and present with similar symptoms. As their treatment is different, especially for dengue haemorraghic fever (DHF) which is a more severe form of dengue, it is important for healthcare workers to differentiate between them. We studied 117 chikungunya and 917 dengue adult patients (including 55 with DHF) by comparing their clinical presentation and developed decision trees to classify them using simple symptoms and laboratory tests. From the study, we found that at their first appearance in hospital, more chikungunya patients had muscle or joint pains, and fewer had a sore throat, cough, nausea, vomiting, diarrhea, stomach pain, loss of appetite or fast heart beat than DF or DHF patients. From the decision trees, of DF versus chikungunya using only platelet level cut-offs, we could correctly classify 89% of the cases. For DHF versus chikungunya using platelet level cut-offs and the presence of
Mosquito-borne chikungunya virus (CHIKV) is a positive-sense, single-stranded RNA virus from the genus Alphavirus, family Togaviridae, which causes fever, rash and severe persistent polyarthralgia in humans. Since there are currently no FDA licensed vaccines or antiviral therapies for CHIKV, the development of vaccine candidates is of critical importance. Historically, live-attenuated vaccines (LAVs) for protection against arthropod-borne viruses have been created by blind cell culture passage leading to attenuation of disease, while maintaining immunogenicity. Attenuation may occur via multiple mechanisms. However, all examined arbovirus LAVs have in common the acquisition of positively charged amino acid substitutions in cell-surface attachment proteins that render virus infection partially dependent upon heparan sulfate (HS), a ubiquitously expressed sulfated polysaccharide, and appear to attenuate by retarding dissemination of virus particles in vivo. We previously reported that, like other ...
Chikungunya causes, symptoms, prevention and treatment in Hindi. चिकनगुनिया का कारण, लक्षण, फैलाव और चिकित्सा की जानकारी. Chikungunya ke dard ka upchar, Chikungunya kaise hota hai. Chikungunya ka gharelu upchar. Chikungunya treatment in Hindi.
Chikungunya virus (CHIKV) is an arthropod-borne agent that causes severe arthritic disease in humans and is considered a serious health threat in areas where competent mosquito vectors are prevalent. CHIKV has recently been responsible for several millions of cases of disease, involving over 40 countries. The recent re-emergence of CHIKV and its potential threat to human health has stimulated interest in better understanding of the biology and pathogenesis of the virus, and requirement for improved treatment, prevention and control measures. In this study, we mapped the binding sites of a panel of eleven monoclonal antibodies (mAbs) previously generated towards the capsid protein (CP) of CHIKV. Using N- and C-terminally truncated recombinant forms of the CHIKV CP, two putative binding regions, between residues 1-35 and 140-210, were identified. Competitive binding also revealed that five of the CP-specific mAbs recognized a series of overlapping epitopes in the latter domain. We also identified a
On September 14, 2015, Pathogens and Global Health journal published online, in an open-access article, details of the first-draft genome sequence of Aedes albopictus, commonly known as the tiger mosquito and responsible for transmitting several deadly diseases to humans, such as dengue fever and chikungunya fever, both carried by viruses, potentially offering hope to millions around the world. "This sequencing…offers great hope to our understanding of the mosquito and our ability to control it, potentially saving millions of lives in many areas of the world," said Professor Andrea Crisanti, Editor-in-Chief of Pathogens and Global Health. The Pathogens and Global Health article provides a first look into the genetics of a most dangerous and invasive insect and the possibility of developing ways to prevent the spread of the dengue and chikungunya fevers that infect millions of people annually. The advance is all the more urgent now, given increasingly high levels of mosquito migration and the ...
Chikungunya virus (CHIKV) is a mosquito-transmitted, single-stranded RNA alphavirus of the Togaviridae family that causes explosive epidemics of a severe febril...
The nsP2 protease of chikungunya virus (CHIKV) is one of the essential components of viral replication and it plays a crucial role in the cleavage of polyprotein precursors for the viral replication process. Therefore, it is gaining attention as a potential drug design target against CHIKV. Based on the recently determined crystal structure of the nsP2 protease of CHIKV, this study identified potential inhibitors of the virus using structure-based approaches with a combination of molecular docking, virtual screening and molecular dynamics (MD) simulations. The top hit compounds from database searching, using the NCI Diversity Set II, with targeting at five potential binding sites of the nsP2 protease, were identified by blind dockings and focused dockings. These complexes were then subjected to MD simulations to investigate the stability and flexibility of the complexes and to gain a more detailed insight into the interactions between the compounds and the enzyme. The hydrogen bonds and hydrophobic
This is a Phase I, open-label, dose-escalation study to examine the safety, tolerability, and immune response to a Virus-Like Particle (VLP) Chikungunya Virus (CHIKV) vaccine in healthy adults ages 18 to 50 years old. The plan is for 25 subjects to receive 3 intramuscular vaccine injections at weeks 0, 4, and 20. The three groups will be enrolled sequentially starting with the lowest dose of 10 micrograms per injection in Group 1.. The hypothesis is that the vaccine is safe and induces immune responses to CHIKV. The primary objective is to evaluate the safety and tolerability of the investigational vaccine, VRC-CHKVLP059-00-VP, at three dosages, 10 micrograms (mcg), 20 mcg, and 40 mcg, in healthy adults. The secondary objective is to evaluate the antibody response against CHIKV VLPs four weeks after the third vaccine injection. The exploratory objectives relate to antigen-specific humoral and cellular immune responses throughout the study. ...
Abstract. Chikungunya virus (CHIKV) is an emerging, mosquito-borne alphavirus that has caused major epidemics in Africa and Asia. We developed chimeric vaccine candidates using the non-structural protein genes of either Venezuelan equine encephalitis virus (VEEV) attenuated vaccine strain TC-83 or a naturally attenuated strain of eastern equine encephalitis virus (EEEV) and the structural genes of CHIKV. Because the transmission of genetically modified live vaccine strains is undesirable because of the potentially unpredictable evolution of these viruses as well as the potential for reversion, we evaluated the ability of these vaccines to infect the urban CHIKV vectors, Aedes aegypti and Ae. albopictus. Both vaccine candidates exhibited significantly lower infection and dissemination rates compared with the parent alphaviruses. Intrathoracic inoculations indicated that reduced infectivity was mediated by midgut infection barriers in both species. These results indicate a low potential for transmission
Link to Pubmed [PMID] - 25739878. Lancet Infect Dis 2015 May;15(5):519-27. Chikungunya is an emerging arthropod-borne disease that has spread from tropical endemic areas to more temperate climates of the USA and Europe. However, no specific treatment or preventive measure is yet available. We aimed to investigate the immunogenicity and safety of a live recombinant measles-virus-based chikungunya vaccine.. http://www.ncbi.nlm.nih.gov/pubmed/25739878 ...
Public health officials in Florida on Thursday confirmed that two people in the state contracted the mosquito-borne chikungunya virus locally, rather than overseas.
1. DomingoE 2010 Mechanisms of viral emergence. Vet Res 41 38. 2. NijhuisMvan MaarseveenNMBoucherCA 2009 Antiviral resistance and impact on viral replication capacity: evolution of viruses under antiviral pressure occurs in three phases. Handb Exp Pharmacol 299 320. 3. ParrishCRKawaokaY 2005 The origins of new pandemic viruses: the acquisition of new host ranges by canine parvovirus and influenza A viruses. Annu Rev Microbiol 59 553 586. 4. ZhangCYWeiJFHeSH 2006 Adaptive evolution of the spike gene of SARS coronavirus: changes in positively selected sites in different epidemic groups. BMC Microbiol 6 88. 5. BraultACPowersAMOrtizDEstrada-FrancoJGNavarro-LopezR 2004 Venezuelan equine encephalitis emergence: enhanced vector infection from a single amino acid substitution in the envelope glycoprotein. Proc Natl Acad Sci U S A 101 11344 11349. 6. TsetsarkinKAVanlandinghamDLMcGeeCEHiggsS 2007 A single mutation in chikungunya virus affects vector specificity and epidemic potential. PLoS Pathog 3 ...
Researchers have developed a new candidate vaccine to protect against chikungunya virus, a mosquito-borne pathogen that produces an intensely painful and often chronic arthritic disease that has stricken millions of people in India, Southeast Asia and Africa.
As of April 7, 2015, a total of 77 chikungunya virus disease cases have been reported to ArboNET from 21 U.S. states for 2015 (Table 1 & Map). All reported cases occurred in travelers returning from affected areas. No locally-transmitted cases have been reported from U.S. states ...
The U.S. Defense Advanced Research Projects Agency is asking innovators to build models that predict the spread of chikungunya virus across the Americas, and take home $150,000.
Dr Richard Gair, the Director of Tropical Public Health Services in Queensland, joined the program to explain. via Chikungunya virus - ABC Queensland - Australian Broadcasting Corporation (ABC).
The history of Chikungunya outbreak in India dates back to 1824 whereas the virus was first isolated in 1963 from Kolkata. 4 The viral activity in humans have been observed till 1971......The present outbreak in India started during December 2005 where the country has so far experienced more than 11,00,000 (11 lakh) of Chikungunya infected cases which still continues. 6 The cases were first reported from Andhra Pradesh, one of the worst affected states. Subsequently reports were poured in from several districts of Karnataka, Kerala, Maharashtra , Orissa, Madhya Pradesh, and Tamil Nadu. Even Andaman experienced the first Chikungunya epidemic during June 2006. Presently the outbreak is underway in western parts of India specially Gujarat and Rajasthan ...
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Réunion Island (Indian Ocean) has been suffering from its first known Chikungunya virus (CHIKV) epidemic since February 2005. To achieve a better understanding of the disease, a questionnaire was drawn up for hospital staff members and their household. CHIKV infected about one-third of the studied population, the proportion increasing with age and being higher in women. Presence of a garden was associated with CHIKV infection. The geographical distribution of cases was concordant with insect vector Aedes albopictus distribution. The main clinical signs were arthralgia and fever. The disease evolved towards full recovery in 34·4% of cases, a relapse in 55·6%, or a chronic form in 10%. Paracetamol was used as a painkiller in 95% of cases, sometimes associated with non-steroidal anti-inflammatory drugs, corticoids, or traditional herbal medicine. The survey provided valuable information on the factors that favour transmission, the clinical signs, the importance of relapses and the therapies used.
In May 2008, a 32 year old Indian male patient presented with chief complaints of a high fever with erythema on ear, severe polyarthritic joint pains & swelling, non pitting pedal oedema, facial puffiness and itching for four days in a Chikungunya viral epidemic outbreak declared region of South Canara district, Karnataka, India. He had no significant past medical and drug history. He had no history of smoking. The patients BMI was 21.6 kg/m2, Blood Pressure was 110/70 mmHg, Pulse rate was 98/min and Respiratory rate was 16/min with no signs of dehydration. The clinical examination of the Respiratory, Cardiovascular, Gastrointestinal and Neurological systems was unremarkable. A provisional clinical diagnosis of Chikungunya was made.. IgM antibody specific to Chikungunya virus was detected using MAC-ELISA. Blood counts revealed leucopenia (2000 cells/micro litre) along with lymphopenia (500cells/Micro litres). The peripheral smear also revealed lymphocytopenia, with the differential lymphocyte ...
Signs of Chikungunya including medical signs and symptoms of Chikungunya, symptoms, misdiagnosis, tests, common medical issues, duration, and the correct diagnosis for Chikungunya signs or Chikungunya symptoms.
chikungunya fever: Viral disease transmitted to humans by infected mosquitoes that is characterized by fever, headache, rash, and severe joint and muscle pain. The name chikungunya, which means
Doctors help you with trusted information about Fever in Chikungunya: Dr. Isaacs on how to cure chikungunya fever: Fever is usually a symptom of infection. If you think you just have a mild illness like the common cold or a simple viral cough then it is probably safe treat the symptoms and see how you go. Medicines like paracetamol and ibuprofen can be effective at lowering your temperature which may make you feel better but wont make you get better faster. Remember to drink plenty of water and rest!
After the bite of an infected mosquito, onset of illness occurs usually between 4 and 8 days, but can range from 2 to 12 days. Chikungunya is characterized by an abrupt onset of fever frequently accompanied by joint pain. Other common signs and symptoms include muscle pain, headache, nausea, fatigue and rash. The joint pain is often very debilitating, but usually lasts for a few days or may be prolonged to weeks. Hence the virus can cause acute, subacute, or chronic disease. Most patients recover fully, but in some cases joint pain may persist for several months, or even years. Occasional cases of eye, neurological, and heart complications have been reported, as well as gastrointestinal complaints. Serious complications are not common, but in older people, the disease can contribute to the cause of death. Often symptoms in infected individuals are mild and the infection may go unrecognized, or be misdiagnosed in areas where dengue occurs. Co-infections of dengue and chikungunya can occur ...
Chikungunya virus (CHIKV) is an arbovirus capable of causing a severe and often debilitating rheumatic syndrome in humans. CHIKV replicates in a wide variety of cell types in mammals, which has made attributing pathologic outcomes to replication at specific sites difficult. To assess the contribution of CHIKV replication in skeletal muscle cells to pathogenesis, we engineered a CHIKV strain exhibiting restricted replication in these cells via incorporation of target sequences for skeletal muscle cell-specific miR-206. This virus, which we term SKE, displayed diminished replication in skeletal muscle cells in a mouse model of CHIKV disease. Mice infected with SKE developed less severe disease signs, including diminished swelling in the inoculated foot and less necrosis and inflammation in the interosseous muscles. SKE infection was associated with diminished infiltration of T cells into the interosseous muscle as well as decreased production of Il1b, Il6, Ip10, and Tnfa transcripts. Importantly, ...
Chikungunya virus (CHIKV) is an arbovirus capable of causing a severe and often debilitating rheumatic syndrome in humans. CHIKV replicates in a wide variety of cell types in mammals, which has made attributing pathologic outcomes to replication at specific sites difficult. To assess the contribution of CHIKV replication in skeletal muscle cells to pathogenesis, we engineered a CHIKV strain exhibiting restricted replication in these cells via incorporation of target sequences for skeletal muscle cell-specific miR-206. This virus, which we term SKE, displayed diminished replication in skeletal muscle cells in a mouse model of CHIKV disease. Mice infected with SKE developed less severe disease signs, including diminished swelling in the inoculated foot and less necrosis and inflammation in the interosseous muscles. SKE infection was associated with diminished infiltration of T cells into the interosseous muscle as well as decreased production of Il1b, Il6, Ip10, and Tnfa transcripts. Importantly, ...
Chikungunya: Virus Chikungunya is a mosquito-borne alpha virus that was first isolated after a 1952 outbreak in modern-day Tanzania .A number of chikungunya outbreaks is due to the change in the genetic sequence which altered the viral coat protein which allows it to multiply more easily in the mosquito cells. The virus uses Asian tiger mosquito (invasive vector ) and the main vector being Aedes aegypti. Precautions need to be taken to stop the growth of the vector to combat the virus spread.. In 2006 there was a large outbreak in India . The virus re-emerged in 2006 after a gap of 32 years and caused an explosive outbreak affecting 13 states. The states first affected were Andhra Pradesh, Karnataka, Maharashtra, Madhya Pradesh, Tamil Nadu, Gujarat and Kerala. The tropical environment of India aids in the mosquito growth and hence spread of the virus.. Now in the year 2016 the outbreak in Delhi and regions near to Delhi is large. According to the Department of Microbiology at AIIMS, 712 samples ...
A promising prophylactic vaccine candidate against chikungunya fever will be tested from August on in a phase II clinical study in Puerto Rico, a chikungunya fever endemic area. Developed by the Austrian biotech company Themis Bioscience GmbH the live attenuated vaccine is considered as one of the most advanced candidates globally. It is based on a standard measles vaccination vector that offers an excellent immunogenicity and safety profile. The vaccine candidate already showed high seroconversion rates in the preceding phase I clinical trial: Up to 100 percent of all vaccinated subjects produced neutralizing antibodies against the chikungunya virus. Currently the vaccine candidate is tested in two parallel phase II studies in both, the USA and Austria/Germany. The additional trial in Puerto Rico is intended to evaluate the influence of previous chikungunya infections on the safety and immunogenicity of the vaccine candidate ...