In the preprimitive streak chick embryo, the search for a region capable of inducing the organizer, equivalent to the Nieuwkoop Center of the amphibian embryo, has focused on Kollers sickle, the hypoblast and the posterior marginal zone. However, no clear evidence for induction of an organizer without contribution from the inducing tissue has been provided for any of these structures. We have used DiI/DiO labeling to establish the fate of midline cells in and around Kollers sickle in the normal embryo. In the epiblast, the boundary between cells that contribute to the streak and those that do not lies at the posterior edge of Kollers sickle, except at stage X when it lies slightly more posteriorly in the epiblast. Hypoblast and endoblast (a second lower layer formed under the streak) have distinct origins in the lower layer, and goosecoid expression distinguishes between them. We then used anterior halves of chick prestreak embryos as recipients for grafts of quail posterior marginal zone; ...
1. The accumulation of [1-(14)C]glycine and the uptake, accumulation, incorporation (into protein, lipid, glycogen) and oxidation of l-[1-(14)C]leucine in 5-day-old chick embryo hearts were investigated in vitro, and the effects of insulin, puromycin and 4-methyl-2-oxopentanoic acid on these processes were studied. 2. With glycine, the ratio of concentration of the labelled amino acid in the cell water to that in medium markedly exceeded unity. Insulin significantly increased this ratio. Puromycin did not prevent the insulin effect. 3. With leucine, the concentration ratio of the labelled amino acid between intracellular and extracellular water approached unity in the absence of puromycin and was doubled by its presence. In neither case did insulin substantially alter this ratio. The addition of 4-methyl-2-oxopentanoic acid had no effect in the absence of insulin, but produced a significant increase of the concentration ratio in the presence of the hormone. 4. Leucine uptake was increased slightly by
We investigated the roles of sodium-calcium exchange, sarcoplasmic reticulum, and mitochondria in Cai homeostasis in cultured chick ventricular cells. Specifically, the influence of low sodium medium on contractile state, calcium fluxes, and cytosolic free [Ca] [( Ca]i) was examined. [Ca]i was measured using fura-2. Mean [Ca]i in control medium was 126 +/- 14 nM. Exposure of cells to sodium-free or sodium- and calcium-free medium (choline-substituted) resulted in contracture development, which returned toward the baseline level over 2-3 minutes. The Nao-free contracture was associated with a tenfold increase in [Ca]i (1,280 +/- 110 nM) followed by a gradual decrease to a level fourfold above control [Ca]i (460 +/- 58 nM). Nao- and Cao-free contracture was associated with a fivefold increase in [Ca]i (540 +/- 52 nM) followed by a rapid decrease to below 80 nM. Sodium-free medium failed to produce an increase in [Ca]i or contracture in cells preexposed to calcium-free medium, although caffeine, ...
MHM is a chicken Z chromosome-linked locus that is methylated and transcriptionally silent in male cells, but is hypomethylated and transcribed into a long non-coding RNA in female cells. MHM has been implicated in both localised dosage compensation and sex determination in the chicken embryo, but direct evidence is lacking. We investigated the potential role of MHM in chicken embryonic development, using expression analysis and retroviral-mediated mis-expression. At embryonic stages, MHM is only expressed in females. Northern blotting showed that both sense and antisense strands of the MHM locus are transcribed, with the sense strand being more abundant. Whole mount in situ hybridization confirmed that the sense RNA is present in developing female embryos, notably in gonads, limbs, heart, branchial arch and brain. Within these cells, the MHM RNA is localized to the nucleus. The antisense transcript is lowly expressed and has a cytoplasmic localization in cells. Mis-expression of MHM sense and antisense
Acetylcholine, acetyl-β-methylcholine and carbamylcholine inhibited sinoatrial pacemaker activity in atria isolated from chick embryos on the 6th, 12th and 18th incubation days. Cardioinhibition persisted throughout the 5-minute exposure to cholinergic agonists in sinoatrial preparations from the 12th and l8th incubation days whereas automaticity recovered completely within 1 to 2 minutes in preparations from the 6th incubation day. Neither hydrolysis by acetylcholinesterase nor release of catecholamines from cardiac stores accounted adequately for the brevity of pacemaker inhibition by choline esters on the 6th incubation day. Evidence suggested that desensitization, most prominently observed with carbamylcholine, restricted the duration of cholinergic inhibition of the pacemaker on the 6th incubation day. Although automaticity recovered completely within 2 minutes after initial application of acetylcholine and carbamylcholine, pacemaker cells were not inhibited by a second application until ...
TY - JOUR. T1 - Molecular analysis of regulative events in the developing chick limb. AU - Mahony, Chris. AU - Vargesson, Neil. PY - 2013/7/1. Y1 - 2013/7/1. N2 - The developing chick limb has the remarkable ability to regulate for the loss of large amounts of mesenchyme and maintain a normal limb pattern in early (Hamburger and Hamilton Stage 19; E3) limbs. How the limb can regulate for tissue loss and why this ability is lost as development proceeds (after Hamburger and Hamilton Stage 21; E3.5) is unclear. We have investigated the origins of cells involved in regulative processes and, for the first time, the molecular changes occurring, and find striking differences between developmental time points just 0.5 days apart. We demonstrate that subtle changes in cell dispersal and cell proliferation occur in HH St21 limbs but not in HH St19 limbs and also demonstrate that there is no net replacement of removed tissue at either HH St21 or St19. We further show that changes in the ...
Synonyms for head process in Free Thesaurus. Antonyms for head process. 352 synonyms for head: skull, crown, pate, bean, nut, loaf, cranium, conk, noggin, noddle, mind, reasoning, understanding, thought, sense, brain, brains.... What are synonyms for head process?
Hamburger Hamilton Stages 5-10== {, ! colspan=3,[[Hamburger Hamilton Stages,Hamburger Hamilton Stages (1951)]]   ,-bgcolor="DDCEF2" , width=150px, ,center>Hamburger Hamilton Stages,/center> , width=100px, ,center>Age,/center> , ,center>Identification of Stages,/center> ,-bgcolor="FAF5FF" , colspan="3" , ,center>Before Laying,/center> ,- , ,center>14,/center> , 50-53 hr , 22 somites; trunk flexure; visceral arches I and II, clefts 1 and 2 ,} ,br> {{HHstage links ...
Paranemin was initially found to copurify with the intermediate filament (IF) proteins vimentin and desmin from embryonic chick skeletal muscle and was described as an IF-associated protein (IFAP). We have purified paranemin from embryonic chick skeletal muscle, prepared antibodies, and demonstrated that they label at the Z-lines of both adult avian and porcine cardiac and skeletal muscle myofibrils. We determined the cDNA sequence of paranemin by immunoscreening a λgt22A cDNA library from embryonic chick skeletal muscle. Northern blot analysis revealed a single transcript of 5.3 kilobases, which is much smaller than predicted from the size of paranemin (280 kDa) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The derived amino acid sequence of paranemin (1,606 residues; 178,161 kDa) contains the conserved IF rod domain (308 amino acids), which has highest homology to the rod domains of nestin and tanabin. Thus, paranemin is an IF protein rather than an IFAP. Sequence analysis ...
Developmental changes in regulation of embryonic chick heart gap junctions.: Embryonic chick myocyte pairs were isolated from ventricular tissue of 4-day, 14-da
Duodena from 20-day-old chick embryos can be maintained in large scale organ culture on specially designed stainless-steel grids in contact with serum-free medium for 48 h with excellent preservation of mucosal structure at both the light and electron microscope levels. Although mitotic rate was subnormal, several other factors attest to the essential viability of the cultured intestine: L-leucine incorporation into protein, as well as the synthesis of a specific vitamin D3-induced calcium-binding protein (CaBP), increased over a 48-h culture period, and the electropotential gradient across the intestine was maintained throughout the culture period as was a concentration gradient for calcium. The tissue responded to vitamin D3 in the medium by synthesizing the calcium-binding protein within 6 h and by exhibiting enhanced 45Ca uptake within 12-24 h. Concentrations of vitamin D3, or its 25-hydroxylated derivative, higher than necessary for CaBP induction, also increased the activity of alkaline ...
The differentiation of chick embryonic skeletal myoblasts results in the formation of myotubes which are the precursors of muscle fibres. The fusion of mononucleated myoblasts represents an apparent switching point in differentiation since it results in both the formation of multinucleated myotubes and the stimulation of muscle specific protein synthesis. The aim of this project has been to examine the biochemical events involved in this process of terminal differentiation by using primary cultures of chick embryonic myoblasts as a model system.. ...
Pretreatment of mouse L cells with mouse interferon (IFN) inhibits the penetration of vesicular stomatitis virus without affecting viral adsorption. The inhibition of virus uptake by IFN is dose dependent and, at the highest dose tested (1,000 units/ml), reaches 65%; 24 hr of treatment with IFN are required for maximal effect. A similar inhibition of uptake of virus occurs in human diploid fibroblasts and primary chicken embryo fibroblasts treated with homologous IFN. No significant inhibition occurs when cells are treated with heterologous IFN. These results document a previously unrecognized antiviral effect of IFN--namely, inhibition at the level of viral uptake.. ...
Hi, Im looking for a commercial source of primary chicken embryo fibroblast (CEF) culture. Any leads? Thanks, Vladimir Yamshchikov ...
In a series of tissue cultures of fragments taken from different parts of young chick embryos, at various stages, it was found that differentiation frequently occurred, the characteristic cells of highly specialised tissues appearing in cultures in which they could not possibly have been present at explantation. The most striking differentiations were the development of red blood corpuscles, capillary vessels, nerve cells with axons and of rhythmically contracting cardiac muscle. This paper describes a simple method by which may be obtained regularly and quickly the development of very large numbers of red cells, and an account is given of the histology of the cultures. It is intended to be introductory to a physiological study, now in progress, of the conditions of hæmatopoiesis Literature. Erythropoiesis in vitro has been reported by several authors, but it has not been thoroughly investigated and, with the exceptions of the works of Slonimski (1930, a, 1931) and Shipley (1915-16), the ...
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Simultaneous left and right atrial surface electrograms were obtained from the right and left atria of 30 chick embryos. These were divided into 3 equal groups that were studied at 15 to 16, 10 to 11, and 5 to 7 days of incubation. The embryos were removed from the shell with their circulation intact and temperature maintained constant. In an early stage of development (5 to 6 days), the left atrium and its appendage were larger than the right, and left preceded right atrial depolarization. At and after 7 days of incubation, the right atrium and its appendage were larger than the left, and right preceded left atrial depolarization. The intervals for interatrial conduction and for atrio-ventricular conduction were about the same at all stages of development. Since the length and width of the heart at 15 to 16 days of incubation were about 3 times those of a heart at 5 to 7 days of incubation, it is proposed that the higher the stage of development of the chick embryo the greater must be the ...
A method for the determination of red cell mass, which employs the use of radioisotopically-labeled erythrocytes, in the embryonated hen egg is described and results of total blood volume, plasma volume, red cell mass and red cell concentration determinations during the period from 9 to 18 days of incubation presented. Considerations of the values obtained in terms of absolute quantities and relative to embryonic mass are discussed. It is suggested that expansion of the red cell mass and somatic growth are portions of a common process and may be subject to a complex of similar regulatory factors in the normal chick embryo.. ...
Fingerprint Dive into the research topics of Presence of PACAP and VIP in embryonic chicken brain. Together they form a unique fingerprint. ...
Fig. 1. Anatomy and gene expression in dissected Xenopus guts. A: Dorsal view of a gut from a 3-day-old embryo. B: Ventral view of a gut from a 3-day-old embryo. C: Gut from a 5-day-old embryo. D: Gut from a 7-day-old embryo. E: Stomach region from a 7-day-old gut. F: Boundary between small and large intestine of 7-day-old gut. G: Activity of endogenous alkaline phosphatase in 3-day gut. H: Activity of endogenous alkaline phosphatase in 7-day gut. Inset shows a section through stained gut (arrow highlights alkaline phosphatase staining (blue) in epithelium, arrowhead highlights mesodermal tissue counterstained green). I: Activity of endogenous alkaline phosphatase in stomach region of 7-day gut (1 and 2 show the first and second expression boundaries). J: Ventral view of IFABP expression in 3-day gut. K: Dorsal view of IFABP expression in 3-day gut. L: IFABP expression in 5-day gut. M: IFABP expression in 7-day gut. N: Endodermin expression in 3-day gut. O: Endodermin expression in 5-day gut. P: ...
Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. Embryonic chick cells were grown ...
Kawakami, Y.; Capdevila, J.; Buscher, D.; Itoh, T.; Rodriguez Esteban, C.; Ng, J.; Izpisua Belmonte, J.C.rlos, 2001: WNT signals control FGF-dependent limb initiation and AER induction in the chick embryo
What might appear in this picture to be an exotic, green glow worm served up on a collard leaf actually comes from something we all know well: an egg. Its a 3-day-old chicken embryo thats been carefully removed from its shell, placed in a special nutrient-rich bath to keep it alive, and then photographed through…
BACKGROUND: Chicken embryos are widely used as a model for studies of obesity; however, no detailed information is available about the dynamic changes of proteins during the regulation of adipose biology and metabolism. Thus, the present study used an isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic approach to identify the changes in protein abundance at different stages of chicken embryonic development. RESULTS: In this study, the abundances of 293 hepatic proteins in 19-day old of chicken embryos compared with 14-day old and 160 hepatic proteins at hatching compared with 19-day old embryos were significantly changed ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
See all results for Memorial Day Chicken Papaya No Flour Recipes. Find more recipes at Foodily.com - the largest recipe search site - and see the ones your friends like.
Since Ms. Cormier was willing to house the chicks on her farm after the class finished with them, I decided that bringing back, and even expanding, this work with live chicks seemed like a really good fit for my vision for the course," said Bailey.. Unfortunately, there have been incidents of chicks passing away. After a power outage in Gelb while the eggs were still incubating, the humidity dropped very low in the incubator. The chicks were already partially hatched during this time, causing the exposed membrane to dry up. According to students interviewed by The Phillipian, two chicks did not hatch as a result of the outage.. The first 36 hours after hatching are a critical time for the chicks - after this period, the birds switch from innate imprinting to associative learning and will not be able to imprint on their "mother." According to students, another factor that facilitates the imprinting process is being rough and showing tough love to the chicks.. Macey Mannion 19 said, "If we spent ...
Hello, everybody! Does anyone have any experience with RNase Protection Assay? I have been doing it for a few months now, using a non-radioactive method for detection (biotin) and I have problems with transferring from the PAGE to a nylon membrane (low efficiency of transfer). My other problem is that after I stain the urea/PAGE with EtBr I see the same band pattern with my control probe (beta-actin) and with my probe (in this case for titin). Its absolutely the same picture-with actin, with titin and with both probes. What I am looking for is differential expression of titin at different stages of primary chick embryo muscle culture and if there is no way of comparing titins expression with that of actin (housekeeping gene), I am in a bad shape. I would greatly appreciate any help! Thanks a lot in advance! Vesselina Ivanova Graduate student in the Department of Biochemistry and Biophysics Iowa State University -- Vesselina Ivanova vivanova at iastate.edu ...
Youre a smart chick wearing Chick with Brains Nightshirt Gifts for Her Let everyone know youre a smart chick wearing this Chick with Brains Nightshirt sporting a geeky little chick embellished on a comfy 100% cotton women Chick with Brains Nightshirt nightshirt. White; one size fits most. Makes an
McKay, Liz. (2007, Fall). Assessment of blood vessel area growth in the developing chick embryo under chronic exposure to 0.5 mL of caffeine. Wheaton Journal of Developmental Biology Research, 2. Retrieved from http://hdl.handle.net/11040/ ...
Origin of lungs, liver, and pancreas in the chick. The mesoderm is shaded; the endoderm dark. lg., One of the lungs; St., stomach; l., liver; p., pancreas." -Thomson, 1916. ...
Hi all. Thanks for all the help last weekend with my little Ameraucana--shes now doing fine! I noticed today that 3 of my chicks have a lump about...
in which she provides a sarcastic quiz with a series of multiple-choice questions as to the books author, cover, main character and themes in order to highlight chick lit as formulaic (Ferriss and Young, 2006: 1).. On the one hand, it is perhaps unsurprising that chick lit has garnered a fair degree of criticism. Along with external clearly gendered cover art and the internal preoccupation with the search for a man, the very term chick lit, as Imelda Whelehan (2005: 171) points out, is simultaneously "interesting and provocative", as the term brings into play both negative and positive connotations, and raises issues of gender and genre (Gormley, forthcoming). As Jane Mills (1989: 47) notes, the term chick, when applied to humans, first entered the English language circa 1400 to mean a child or young girl. In the 1500s, chick became, "a term of endearment applied to a female sweetheart"; yet by the early 1900s the term had "degenerated" to denote, "a young promiscuous woman" (ibid.). In the ...
One was named Spotty because he had black spots on him. I loved listening to the sound of the chicks chirping. They are so cute!" "First the chicks were a little germ spot in an egg and then the spot got bigger and bigger and when it got so crammed up in there the chick popped out. It takes 21 days for them to hatch. How a chick gets formed is a hen meets a rooster." " The parts of the egg are called the air pocket, the yolk, chilaza, and the embryo." By Maisie ...
We have made ten attempts to cultivate vaccine virus in tissue extracts prepared according to the method described by Eagles and Kordi (4). Renal, testicular, and chick embryo extracts were employed with a dermal strain of vaccine virus and with the Levaditi strain of neuro-vaccine virus. In no instance were we able to show that the virus multiplied in the extract media. Both of these strains of virus, however, multiplied in media containing bits of minced viable tissue. Furthermore, treatment of rabbit testicular tissue and chick embryo tissue in the manner described by Eagles and Kordi for the preparation of the extracts leaves some cells not only alive but capable of proliferation. Although the results of our work are not in accord with those obtained by Eagles and Kordi, we offer no explanation for the discrepancy. Nevertheless, one cannot examine the results of our work recorded in the six tables without recognizing the fact that in the types of media used the presence of viable cells ...
E hyaloid vessels. doi:10.1371/journal.pone.0053970.gused immunohistochemistry of melanocyte and melanoma cell specific markers HMB45 and Melan-A. Since in the early chick embryo neural crest cells have not yet differentiated, they do not express markers of the…. ...
I have just cleared out eggs from an old coop. I thought they were old eggs, but when I threw them out I found that one of them had an embryo in it.
Despite the relatively large size, the whole mounts (item# 311676) are cleared and stained. Serial sections (item# 311688) show the beginning of most of the structures of the adult eye.
A transverse section through an embryo chick (26 hours). Labels: a, epiblast; b, mesoblast; c, hypoblast; d, central portion of mesoblast, which is here fused with epiblast; e, primitive groove; f, dorsal ridge.. ...
Ive got a big emergency with one of my chicks. When it hatched, it hadnt absorbed the yolk fully and it still had it attached. The chick was very...
Conversion of tbsp amounts of BABYFOOD,DINNER,SWT POTATOES&CHICK,STR into jar Beech-Nut Stage 2 (4 oz) measuring units. Exchange amounts between 1 tbsp and 1 or multiples of jar Beech-Nut Stage 2 (4 oz) measure of BABYFOOD,DINNER,SWT POTATOES&CHICK,STR product.
The Department of Biological Regulation is comprised of approximately 160 people organized in 13 research groups. We are located in the Candiotty building, which is equipped with all the facilities required for running an excellent research. Our research is concentrated on the regulation of processes responsible for the concerted action of cells, tissues, and organs. A diversity of methodologies and experimental approaches are used in order to tackle these issues. These include biochemical, molecular, and physiological methods, organ and tissue cultures, whole animal studies in mice, flies, zebra fish, and chick embryos.. ...
A Dutch start-up has said it is close to developing a commercially viable solution to sexing chicks just nine days into their incubation. In-Ovo says it ha
Designed to prevent baby chicks from drowning, yet still large enough for adult birds to drink from. Includes translucent plastic 1 qt. jar. Desired Date of Arrival can be entered …
Designed to prevent baby chicks from drowning, yet still large enough for adult birds to drink from. Will fit standard mason jar.
|Those little pro ana sweethearts often use pictures of fat girls to cause what they call reverse thinspo. I thought that I Fat Bastard would post up some pictures of emaciated skinny chicks and fat girls to provide some fatsporation and reverse fatspo for our million of female readers.Id bone here but that is only…
Fascinating ebony chick with round black butt and shaved wet pussy has some fun with a European guy and gives her needy twat for hardcore interracial shagging
Definition: A variant of group of so-called fusion anomalies, in which both kidneys are fused together in early embryonic life.. ...
Six Western women share their unique story, experience and give advice on dating Japanese men. Cultural differences can cause problems in the relationship. Do online dating websites work? Its time for a frank discussion! What I learned from interviews was that online dating is equally painful for men and for
Im pondering new features for this site - for one thing, I promise to get a better picture up there soon! I would like to have a more interactive format, perhaps a forum for debates, seeing as new developments in science, health and technology do not come without controversy ...