To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...

[100 Pages Report] Check for Discount on Global Hypothermic Machine Perfusion Preservation Sales Market Report 2021 report by QYResearch Group. Notes: Sales, means the sales volume of Hypothermic Machine Perfusion...

TY - JOUR. T1 - Cardiac transplantation after prolonged graft preservation with the University of Wisconsin solution. AU - Jeevanandam, V.. AU - Auteri, J. S.. AU - Sanchez, J. A.. AU - Hsu, Daphne T.. AU - Marboe, C.. AU - Smith, C. R.. AU - Rose, E. A.. PY - 1992. Y1 - 1992. N2 - We tested the ability of University of Wisconsin solution to extend hypothermic preservation of the nonperfused heart during orthotopic baboon allotransplantation. Seven baboons received hearts after cardioplegia and storage (4° C) with University of Wisconsin solution, with a preservation time of 14.2 ± 1.6 hours. One animal died as a result of a technical error. Six survivors were immunosuppressed for 45 days and then put to death. Preservation did not alter heart weight or histologic features according to light and electron microscopy. Animals were weaned from bypass and returned to their cages without intravenous support within 3.9 ± 0.8 hours. Weekly biopsies, electrocardiograms, enzyme analyses, ...

A pathophysiologic study of the kidney tubule to optimize organ preservation solutions. Background. Tissue damage at the time of organ transplantation has a negative impact on the subsequent success of the procedure, both in the immediate and longer term. Hypothermia is the principal element used to prolong organ viability ex vivo, but paradoxically also induces cellular edema through inhibition of energy-dependent adenosine triphosphatases (ATPases). This induces an electrolyte imbalance that leads to fluid influx and cell swelling. It is important, therefore, that improvements are made in the preservation of ischemic organs to reduce this injury. Methods. This study has applied a novel in vitro system to model cold and warm ischemic-induced renal tubule swelling that characterizes tissue damage in ischemia/reperfusion injury. Biochemical blockade of ATPases in this system using strophanthidin modeled the effects of energy depletion and induced cell swelling. By measuring such tubule swelling ...

TY - JOUR. T1 - An observational study of Donor Ex Vivo Lung Perfusion in UK lung transplantation: DEVELOP-UK. AU - Fisher, Andrew. AU - Andreasson, Anders. AU - Chrysos, Alexandros. AU - Lally, Joanne. AU - Mamasoula, Chrysovalanto. AU - Exley, Catherine. AU - Wilkinson, Jennifer. AU - Qian, Jessica. AU - Watson, Gillian. AU - Lewington, Oli. AU - Chadwick, Thomas. AU - McColl, Elaine. AU - Pearce, Mark. AU - Mann, Kay. AU - McMeekin, Nicola. AU - Vale, Luke. AU - Tsui, Steven. AU - Yonan, Nizar. AU - Simon, Andre. AU - Marczin, Nandor. AU - Mascaro, Jorge. AU - Dark, John. PY - 2016/11/1. Y1 - 2016/11/1. N2 - Background - Many patients awaiting lung transplantation die before a donor organ becomes available. Ex vivo lung perfusion (EVLP) allows initially unusable donor lungs to be assessed and reconditioned for clinical use. Objective - The objective of the Donor Ex Vivo Lung Perfusion in UK lung transplantation study was to evaluate the clinical effectiveness and cost-effectiveness of EVLP in ...

TY - JOUR. T1 - Nicardipine reverses vasoactivity associated with university of Wisconsin solution in the rat peripheral circulation. AU - Raveh, Y.. AU - Lubarsky, D. A.. AU - Pretto, E. A.. AU - Proctor, K. G.. N1 - Funding Information: This work was supported by internal departmental funding. KGP is supported, in part, by grant N0001406160670 from the Office of Naval Research . Copyright: Copyright 2012 Elsevier B.V., All rights reserved.. PY - 2011/9. Y1 - 2011/9. N2 - Background: The rapid uniform delivery of University of Wisconsin solution (UW) to the microcirculation may be compromised by its vasoactivity. Methods: In 2 different rodent models, we tested whether UW-mediated vasoconstriction could be reversed with nicardipine. Results: In the perfused, splanchnic circulation, intravascular control solutions (lactated Ringers [LR], Hextend [HEX], histidine-tryptophan-ketoglutarate [HTK]) or UW (± nicardipine) evoked pressure changes in 3 protocols (series 1; n = 35). In the cremaster ...

Human serum albumin (HSA) is a mixture of human mercaptalbumin (HMA, reduced form) and nonmercaptalbumin (HNA, oxidized form). We have developed a convenient high-performance liquid chromatographic (HPLC) system for the separation of HSA into HMA and HNA, and studied the mercapt,==,nonmercapt conversion (i.e., dynamic change in the redox state) of HSA. Examination of long-term sample preservation temperature on the redox state of HSA is of fundamental importance for analysis of defense systems against oxidants in humans. The HMA fraction of HSA (f(HMA)) was markedly decreased, i.e., the redox state of HSA samples was more oxidized, when they were kept even at -20 degrees C for 170 days. Moreover, the redox states of five commercial HSA products were analyzed and the results were compared with those for normal control subjects. Surprisingly, marked decreases in f(HMA) value for all commercial HSA products were observed. ...

Until now, a comprehensive textbook covering both ordinary differential equations (ODEs) and partial differential equations (PDEs) didn't exist. We propose a neural network based approach for extracting models from dynamic data using ordinary and partial differential equations. Ordinary and Partial Differential Equations Proceedings of the Fourth Conference Held at Dundee, Scotland March 30 - April 2, 1976 E.g. This textbook can be tailored for courses in numerical differential equations and numerical analysis as well as traditional courses in ordinary and/or partial differential equations. 6) (vi) Nonlinear Differential Equations and Stability (Ch. Intended primarily for engineering and science students. Maple is the world leader in finding exact solutions to ordinary and partial differential equations. An ordinary differential equation involves a derivative over a single variable, usually in an univariate context, whereas a partial differential equation involves several (partial) derivatives ...

Introduction. Controlled oxygenated rewarming of cold stored livers by machine perfusion demonstrated a strong protective effect in preclinical studies. Here we report on the first clinical application of this new concept in a pilot series of 6 marginal donor grafts.. Methods. Between 03/2014 and 05/2014 six distally procured livers from DBD-donors, which had been rejected by other transplant centers were subjected to the controlled rewarming protocol before transplantation. Immediately after arrival in our transplant center the hepatic artery and portal vein were cannulated. Gentle rewarming of the graft was effectuated thereafter by pressure controlled oxygenated machine perfusion (MP) via portal vein and hepatic artery (Organ Assist, Groningen, NL) while gradually increasing perfusate temperature up to 20°C. Custodiol-N was used as preservation solution. Real-time biochemical analysis of perfusate parameters was carried out in constant time intervals during the controlled ...

α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their catalytic activity is of great interest in numerous biotechnological applications, especially those in the food industry directed to the degradation of oligosaccharides derived from raffinose. The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. The MEL1 gene coding for the α-galactosidase of S. cerevisiae (ScAGal) was cloned and expressed in the S. cerevisiae strain BJ3505. Different constructions were designed to obtain the degree of purification necessary for enzymatic characterization and to improve the productive process of the enzyme. ScAGal has greater specificity for the synthetic substrate p-nitrophenyl-α-d-galactopyranoside than for natural substrates, followed by the natural glycosides, melibiose, raffinose

More than two million American women are breast cancer survivors. Approximately one-third of these women are premenopausal at diagnosis and face issues related to reproduction as they undergo cancer treatment. Ovarian function after breast cancer diagnosis has implications on breast cancer prognosis, choice of adjuvant therapy and reproductive issues such as desire for fertility or concerns about menopause. Therefore, tools to accurately predict ovarian function in breast cancer survivors could significantly impact physicians and patients in counseling, medical and surgical treatment choices, and consideration of fertility preservation options.. The goal of this proposal is to identify pre-chemotherapy hormonal, genetic and ovarian imaging markers that can predict ovarian failure and characterize the course of ovarian function after chemotherapy. The investigators plan to follow a group of young women from breast cancer diagnosis to five years after chemotherapy. The investigators will study the ...

PDE #10 Partial Differential Equation in Hindi|Form of PDE By Eliminating Arbitray Function|NP Bali Hello student welcome to JK SMART CLASSES , I will be discuss Engineering Mathematics 3 Partial differential equations in Hindi .Now in this video I will briefly explained PDE #10 Formation Partial Differential Equation in Hindi By Eliminating Arbitary Function|PTU|NP BALI|JK Smart Classes for B.tech and m.tech student whose study in various universities like Punjab Technical university (PTU), Gujarat Technological University (GTU), Visvesvaraya Technological University (vtu), Rajasthan Technical University (RTU), kurukshetra university (KU) and Mumbai university etc.for exam point of view Partial differential equations in Hindi chapter for engineering mathematics 3 in very important chapter for pass point of view as well as for getting good marks in Engg math 3.so student please more focus on Partial differential equations in Hindi chapter for success in exam So

Definition of HTK solution in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is HTK solution? Meaning of HTK solution as a legal term. What does HTK solution mean in law?

Whole cell and surface proteomes were analyzed together with adhesive properties of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) grown on the emerging prebiotic raffinose, exemplifying a synbiotic. Adhesion of NCFM to mucin and intestinal HT-29 cells increased three-fold after culture with raffinose versus glucose, as also visualized by scanning electron microscopy. Comparative proteomics using 2D-DIGE showed 43 unique proteins to change in relative abundance in whole cell lysates from NCFM grown on raffinose compared to glucose. Furthermore, 14 unique proteins in 18 spots of the surface subproteome underwent changes identified by differential 2DE, including elongation factor G, thermostable pullulanase, and phosphate starvation inducible stress-related protein increasing in a range of +2.1 − +4.7 fold. By contrast five known moonlighting proteins decreased in relative abundance by up to −2.4 fold. Enzymes involved in raffinose catabolism were elevated in the whole cell ...

TY - JOUR. T1 - Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. AU - Kokotilo, Matthew S.. AU - Carter, Jodi. AU - Thiesen, Aducio. AU - Chen, Ming H.. AU - Ochs, Angela. AU - Khadaroo, Rachel G.. AU - Churchill, Thomas A.. PY - 2010/12/1. Y1 - 2010/12/1. N2 - Introduction: Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage. Methods: Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a 'back table' intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES ( n= 6 per ...

TY - JOUR. T1 - Glutathione supplementation to University of Wisconsin solution causes endothelial dysfunction. AU - Tambyraja, A L. AU - Mitchell, R. AU - Driscoll, P J. AU - Deans, C. AU - Parks, R W. AU - Rahman, I. AU - Megson, I L. PY - 2007. Y1 - 2007. N2 - Glutathione (GSH) is added to University of Wisconsin (UW) organ preservation solution to protect against oxidative stress. This study assesses the effect of GSH-supplementation on endothelial function in tissues subjected to cold ischaemia and compares its effects to a mono-ethyl ester equivalent (GSH-MEE) and S-nitrosated GSH (GSNO).. AB - Glutathione (GSH) is added to University of Wisconsin (UW) organ preservation solution to protect against oxidative stress. This study assesses the effect of GSH-supplementation on endothelial function in tissues subjected to cold ischaemia and compares its effects to a mono-ethyl ester equivalent (GSH-MEE) and S-nitrosated GSH (GSNO).. U2 - 10.1016/j.trim.2007.06.002. DO - ...

TY - JOUR. T1 - Long-term lung preservation with the PAF antagonist BN 52021. AU - Conte, John. AU - Katz, Nevin M.. AU - Wallace, Robert B.. AU - Foegh, Marie L.. PY - 1991/1/1. Y1 - 1991/1/1. N2 - Platelet activating factor (PAF, l-alkyl-2(R)-acetyl- glycero-3-phosphorylcholine) is a phospholipid that is released by a variety of cells. The similarity between the pathophysiological effects of PAF and posttrans-plant pulmonary dysfunction led to an evaluation of a PAF antagonist as an adjunct to lung preservation. The ginkgolide B, BN 52021, was selected as the PAF antagonist to be studied because of the large data base available on this compound. BN 52021 was given to the donor and recipient (10 mg/kg i.v.) prior to harvest and transplantation and was included in 1 L of preservation solution (10 mg/kg) used for flushing the pulmonary artery and for storage. Left single-lung transplantation was performed following a 22-hr preservation period at 10°C. Arterial oxygen tension (pO2), pulmonary ...

TIANCHI Small Liquid Nitrogen Tanks 3 Liter LN2 Dewar Container Cryo Tank. Net Weight(KG):3.2. Gross Weight(KG):5.0. Static Power Dissipation(Kg/d):0.08. This Small Liquid Nitrogen Tanks 3 Liter is made of high-strength, special corrosion resistant aluminum alloy, light weight and easy to carry. Liquid nitrogen storage containers is well made, very good insulation, integrated molding, weld the small, solid and reliable, guarantee vacuum and prevent leakage. Liquid nitrogen container low loss, more than 5 years of vacuum quality.. Liquid nitrogen storage tank can be stored for a long biological specimens, semen, embryos, spawn, vaccines, skin, organ and other biological material. Products three guarantees service, for each customer to establish user files, and enjoy lifelong technical services. This LN2 Dewar Container is only suitable for the static storage of liquid nitrogen, not suitable for the transport of liquid nitrogen. This cryogenic container ships empty. It can be filled anywhere that ...

Paraovarian/paratubal cysts constitute 5-20% of all adnexal lesions and typically originate from the paramesonephric or Müllerian duct. The primary epithelial tumors arising from paraovarian cysts account for 25% of the cases, but giant cystadenomas of paraovarian origin are extremely uncommon during childhood and adolescence with very few cases reported in the literature. We present the case of a 15-year-old female that presented with a bulky mass in the abdomen and pelvis. An initial clinical and radiological examination indicated an ovarian cyst measuring ∼25x20 cm. However, explorative laparotomy revealed a giant paratubal cyst that was successfully treated with complete excision using fertility-sparing surgery. Histopathological examination was consistent with a serous cystadenoma. The postoperative course was uneventful and the girl was discharged on the seventh postoperative day. At the follow-up of six months, the patient was doing well. Due to their rarity and enormous size, the ...

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Several stochastic simulation algorithms (SSAs) have been recently proposed for modelling reaction-diffusion processes in cellular and molecular biology. In this talk, two commonly used SSAs will be studied. The first SSA is an on-lattice model described by the reaction-diffusion master equation. The second SSA is an off-lattice model based on the simulation of Brownian motion of individual molecules and their reactive collisions. The connections between SSAs and the deterministic models (based on reaction-diffusion PDEs) will be presented. I will consider chemical reactions both at a surface and in the bulk. I will show how the 'microscopic' parameters should be chosen to achieve the correct 'macroscopic' reaction rate. This choice is found to depend on which SSA is used. I will also present multiscale algorithms which use models with a different level of detail in different parts of the computational domain ...

TY - JOUR. T1 - Hepatic protein synthesis rate of liver specimens as a predictor of viability in rat cold ischemia liver transplantation model. AU - Matsui, Yoshifumi. AU - Asano, Takehide. AU - Nakagohri, Toshio. AU - Yokoro, Yoshiharu. AU - Kainuma, Osamu. AU - Kenmochi, Takashi. AU - Isono, Kaich. PY - 1997/11. Y1 - 1997/11. N2 - Background/Aims: We have previously reported that the hepatic protein synthesis rate, calculated as the uptake rate of L-[4.5 3H] leucine by the fraction during a 10-min incubation of a 16-G needle biopsy specimen of liver tissue, represents a high level of liver function and is therefore useful for evaluating liver function. We investigated the hepatic protein synthesis rate level in a pretransplant liver to learn if it might predict the outcome in a rat orthotopic liver transplantation model. Methods: Grafts were stored, liver specimens were obtained using a 21-G Chiba type II skinny needle, and the hepatic protein synthesis rate was calculated. Subsequently, liver ...

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Recent years have seen an explosion of interest in stochastic partial differential equations where the driving noise is discontinuous. In this comprehensive monograph, two leading experts detail the evolution equation approach to their solution. Most of the results appeared here for the first time in book form. The authors start with a detailed analysis of Lévy processes in infinite dimensions and their reproducing kernel Hilbert spaces; cylindrical Lévy processes are constructed in terms of Poisson random measures; stochastic integrals are introduced. Stochastic parabolic and hyperbolic equations on domains of arbitrary dimensions are studied, and applications to statistical and fluid mechanics and to finance are also investigated. Ideal for researchers and graduate students in stochastic processes and partial differential equations, this self-contained text will also interest those working on stochastic modeling in finance, statistical physics and environmental science. ...

The emerging of nanotechnology has increasingly gained expansions and applications in various materials science research and development. However, the exposure to nanoparticles and engineered nanomaterials can lead to adverse biological effects because the small sizes of nanoparticles can enter the human body and deposit in the organs or translocate from the intake area to the secondary organs and can cause inflammation. One of the most used nanoparticles is TiO2, which is commonly found in skin care and household products. It is still unclear how TiO2 nanoparticles are remained in human bodies after exposing. In the present study, we develop a physiologically-based pharmacokinetic (PBPK) model to predict the bio-distribution of TiO2 concentrations in rat tissues. The model is validated with an existing in-vivo study in rats. We also extend our PBPK model to predict cell death caused by TiO2 nanoparticles in the rat liver using a dose-response model. The dose-response model accounts for the interplay

The purpose of this study was to analyze the possible effects of machine perfusion (MP) versus cold storage (CS) on delayed graft function (DGF) and early graft survival in expanded criteria donor kidneys (ECD). As part of the previously reported international randomized controlled trial 91 consecutive heart-beating deceased ECDs--defined according to the United Network of Organ Sharing definition--were included in the study. From each donor one kidney was randomized to MP and the contralateral kidney to CS. All recipients were followed for 1 year. The primary endpoint was DGF. Secondary endpoints included primary nonfunction and graft survival. DGF occurred in 27 patients in the CS group (29.7%) and in 20 patients in the MP group (22%). Using the logistic regression model MP significantly reduced the risk of DGF compared with CS (OR 0.460, P=0.047). The incidence of nonfunction in the CS group (12%) was four times higher than in the MP group (3%) (P=0.04). One-year graft survival was significantly

Fertility and pregnancy issues are of key importance for young breast cancer patients. Despite several advances in the field, there are still multiple unmet needs and barriers in discussing and dealing with these concerns. To address the significant challenges related to fertility and pregnancy issues, the PREgnancy and FERtility (PREFER) study was developed as a national comprehensive program aiming to optimize care and improve knowledge around these topics. The PREFER study is a prospective cohort study conducted across several Italian institution affiliated with the Gruppo Italiano Mammella (GIM) group evaluating patterns of care and clinical outcomes of young breast cancer patients dealing with fertility and pregnancy issues. It is composed of two distinctive studies: PREFER-FERTILITY and PREFER-PREGNANCY. The PREFER-FERTILITY study is enrolling premenopausal patients aged 18-45 years, diagnosed with non-metastatic breast cancer, who are candidates to (neo)adjuvant chemotherapy and not previously

Congratulations to Dr. Teresa Woodruff, the Director of the Oncofertility Consortium and the Dean of the Graduate School at Northwestern University for her most recent award. Dr.

Have you just recently discovered that your milk, eggs, and other refrigerated items seem a little warmer than usual? This is definitely an issue, as specific foods require to be stored at the optimal temperature to avoid ruining. Have you examined the freezer temperature, but it's still cold? If so, you might have a refrigerator stop cooling on refrigerator side problem on your hands.. So what triggers the refrigerator to stop cooling on refrigerator side issue? There are in fact several things that can trigger the refrigerator not cooling issue. Much depends on the make, design, and brand name of your refrigerator, however there are a couple of more common failures that lead to a failing fridge. Repair work range from defrosting refrigerator evaporator coils to replacing the refrigerator thermostat.. We've detailed a few of the most typical factors a refrigerator isn't cooling on refrigerator side for you here. Use this list to repair and see if you can fix it yourself, or click fridge repairs ...

BACKGROUND: The standard preservation solution used during organ procurement and preservation of most organs is the University of Wisconsin (UW) solution. Despite its superiority over other cold storage solutions, the inclusion of hydroxyethyl starch (HES) as one of the components of the UW solution has been both advocated and denied. This study determined whether HES had any effect on red blood cell (RBC) aggregability and correlated aggregation parameters with HES molecular weight. METHODS: Human RBC aggregability and deformability were investigated in vitro, at 4 degrees C, with a laser-assisted optical rotation cell analyzer. The study of RBC aggregation in a binary HES-HES system gave an indication about the nature of HES-RBCs interactions. Bright field microscopy and atomic force microscopy were used to morphologically characterize the aggregates size and form. RESULTS: High molecular weight HES and UW solution had a potent hyperaggregating effect; low molecular weight HES had a hypoaggregating

Preservation of macroparasites by infiltrating the polymer in the tissues can defy the inherited shortcoming of classical wet preservation method

TY - JOUR. T1 - Ex vivo application of carbon monoxide in UW solution prevents transplant-induced renal ischemia/reperfusion injury in pigs. AU - Yoshida, J.. AU - Ozaki, K. S.. AU - Nalesnik, M. A.. AU - Ueki, S.. AU - Castillo-Rama, M.. AU - Faleo, G.. AU - Ezzelarab, M.. AU - Nakao, A.. AU - Ekser, B.. AU - Echeverri, G. J.. AU - Ross, M. A.. AU - Stolz, D. B.. AU - Murase, N.. PY - 2010/4. Y1 - 2010/4. N2 - I/R injury is a major deleterious factor of successful kidney transplantation (KTx). Carbon monoxide (CO) is an endogenous gaseous regulatory molecule, and exogenously delivered CO in low concentrations provides potent cytoprotection. This study evaluated efficacies of CO exposure to excised kidney grafts to inhibit I/R injury in the pig KTx model. Porcine kidneys were stored for 48 h in control UW or UW supplemented with CO (CO-UW) and autotransplanted in a 14-day follow-up study. In the control UW group, animal survival was 80% (4/5) with peak serum creatinine levels of 12.0 ± 5.1 ...

For freezing ice cream or other confections in a refrigerator's freezer area, a refrigerator tray was a rectangular, shallow, open container. The ice cube tray (often provided with the refrigerator) began, around the mid-to-late thirties, to be fitted with a removable divider so that it doubled as the refrigerator tray.. Ice cube trays with removable dividers are referred to as "refrigerator trays" and were designed specifically for freezing desserts.. The terms "refrigerator tray", "freezer tray", and "ice cube tray" were used somewhat interchangeably for making desserts with these trays, at least during the period after ice cube trays with removable dividers were included with refrigerators and until the included ice cube trays became one-piece plastic trays.. The Carnation Fun to Cook Book, for example, has a recipe for "tropical freeze" on page 40; for the final freeze, the recipe is poured into "two small (or 1 large) refrigerator trays". The drawings accompanying the recipe show shallow, ...

Natural gas is produced through thermogenic and biogenic processing over millions of years and natural gas transmission plants and storage solutions can be as difficult to achieve as the recovery and processing activities. The gas can be partially processed before being piped to the refineries, were the natural gas is further processed to produce pipeline quality gas. Transmission is either by pipelines from the processing plants to the consumers or by conversion of the gas to liquid natural gas and transported in specialist LNG carriers. Storage can be in natural underground caverns or aquifers or depleted oil/gas reservoirs.

The shortage of donor organs for liver transplantation requires investigations into expanding the donor pool. Non-heart beating donor (NHBD) livers are an untapped source of organs. NHBD organs are those that have had a period of warm ischemia prior to cold preservation. A main concern of NHBD livers is that there is a depletion of energy stores during the warm ischemia and subsequent cold storage. This study is to investigate the use of hypothermic machine perfusion to regenerate energy stores in the liver during 5hr storage and after 1hr rewarming in a rat transplantation model. Results will be compared with control. (no warm ischemia) and simple cold stored tissues.. Copyright © 2003 by ASME ...

TY - JOUR. T1 - Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples. AU - Bennike, Tue Bjerg. AU - Kastaniegaard, Kenneth. AU - Padurariu, Simona AU - Gaihede, Michael. AU - Birkelund, Svend. AU - Andersen, Vibeke. AU - Stensballe, Allan. PY - 2016. Y1 - 2016. N2 - Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described.Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30 min. The protein content of the samples was characterized by high throughput quantitative proteomics.We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor ...

Turn the filter to the left and pull. Filters located in the base grille of your refrigerator These filters have a reusable knob. My husband and I have attempted to replace the filter (as we have done so successfully in the past three years), and we cannot get the filter to install. Locate your water filter cover in the upper right-hand corner of your refrigerator interior. Changing your GE GSWF refrigerator water filter on a regular basis. Take the new filter to the tap and fill it with water. If the filter tab in your refrigerator is facing outward, push on it and pull the cover down. How do I replace the water filter in my refrigerator? * (*Contaminants or other substances removed or reduced by this water filter are not necessarily in all users' water) Changing your GE GSWF filter is critical when your refrigerator water filter has gotten old, or has a spent life in your refrigerator. Turn off water. When replacing the water filter, you must first close the water … Get the GE refrigerator ...

The use of energy crops and agricultural residues is expected to increase to fulfil the legislative demands of bio-based components in transport fuels. Ensiling methods, adapted from the feed sector, are suitable storage methods to preserve fresh crops throughout the year for, for example, biogas production. Various preservation methods, namely ensiling with and without acid addition for whole crop maize, fibre hemp and faba bean were investigated. For the drier fibre hemp, alkaline urea treatment was studied as well. These treatments were also explored as mild pretreatment methods to improve the disassembly and hydrolysis of these lignocellulosic substrates. The investigated storage treatments increased the availability of the substrates for biogas production from hemp and in most cases from whole maize but not from faba bean. Ensiling of hemp, without or with addition of formic acid, increased methane production by more than 50% compared to fresh hemp. Ensiling resulted in substantially increased

The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia in either saline or Custodiol-HTK solution. Mitochondrial function was measured in situ in permeabilized fibers by high-resolution respirometry. Mitochondrial respiratory capacities (maximal respiration rates) were similar in the right and left ventricle in controls and after 14 h of cold storage were significantly better preserved in Custodiol-HTK than in saline solution. Mitochondrial respiration rates in various blood vessels including aorta, arteries and veins were less than 5% of myocardium rates. In contrast to the pig heart, in some blood vessels, like veins, mitochondrial function remained stable even after 24 h of cold ischemia. HTK

Successful pregnancy and delivery following combined treatment of In Vitro Maturation (IVM) and Testicular Sperm Extraction (TESE). (Fuchinoue K, Nakajo Y, Yagi A, Takeda M, Kyono K. Successful pregnancy and delivery following combined treatment of in vitro maturation (IVM) and testicular sperm extraction (TESE): a case report. J Assist Reprod Genet. 2004;21(10):371-3).. Jaques Donnez reporting on the first Live birth after orthotopic transplantation of cryopreserved ovarian tissue (Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, van Langendonckt A. vebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004:364(9443):1405-10).. Gardner and colleagues performed the world's first prospective single blastocyst trial, which showed the feasibility of SBT and in keeping high pregnancy rates (Gardner DK, Surrey E, Minjarez D, Leitz A, Stevens J, Schoolcraft WB. Single blastocyst transfer: a prospective randomized trial. Fertil Steril. ...

TY - JOUR. T1 - Surgical issues in lung transplantation. T2 - Options, donor selection, graft preservation, and airway healing. AU - Daly, Richard C.. AU - Mcgregor, Christopher G.A.. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 1997. Y1 - 1997. N2 - To present an overview of the surgical issues in lung transplantation, including the historical context and the rationale for choosing a particular procedure for a specific patient, we reviewed and summarized the current medical literature and our personal experience. Several surgical options are available, including single lung transplantation; double lung transplantation; heart-lung transplantation; bilateral, sequential single lung transplantation; and (recently) single lobe transplantation. Although single lung transplantation is preferred for maximal use of the available organs, bilateral lung transplantation is necessary for septic lung diseases and may be appropriate for pulmonary hypertension and bullous ...

TY - JOUR. T1 - Recurrent hepatitis C posttransplant. T2 - Early preservation injury may predict poor outcome. AU - Watt, Kymberly D.S.. AU - Lyden, Elizabeth R.. AU - Gulizia, James M.. AU - McCashland, Timothy M.. PY - 2006/1. Y1 - 2006/1. N2 - Organ cold/warm ischemia is thought to be a risk factor for increased severity of recurrence of hepatitis C (HCV) post liver transplantation. We had noted some HCV patients with preservation injury (PI) to have particularly poor outcomes. Our goal was to determine if PI on biopsy in HCV patients is associated with earlier, more rapidly progressive recurrence or graft and patient survival. Sixty-nine patients from the University of Nebraska transplant database were included: 23 HCV patients with PI (group = 1), 23 non-HCV patients with PI (group = 2), and 23 HCV patients without PI (group = 3). Patient groups were matched for gender, age, immunosuppression, and time of transplantation for analysis. No difference in time to recurrence was noted between ...

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TY - JOUR. T1 - Nitrite reduces acute lung injury and improves survival in a rat lung transplantation model. AU - Sugimoto, R.. AU - Okamoto, T.. AU - Nakao, A.. AU - Zhan, J.. AU - Wang, Y.. AU - Kohmoto, J.. AU - Tokita, D.. AU - Farver, C. F.. AU - Tarpey, M. M.. AU - Billiar, T. R.. AU - Gladwin, M. T.. AU - McCurry, K. R.. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2012/11. Y1 - 2012/11. N2 - Ischemia/reperfusion injury (IRI) is the most common cause of early mortality following lung transplantation (LTx). We hypothesized that nitrite, an endogenous source of nitric oxide (NO), may protect lung grafts from IRI. Rat lung grafts were stored in preservation solution at 4°C for 6 hours. Both grafts and recipients were treated with nitrite. Nitrite treatment was associated with significantly higher levels of tissue oxygenation, lower levels of cytokines and neutrophil/macrophage infiltration, lower myeloperoxidase activity, reduced oxidative injury and increased ...

Maca's use goes back to around 3800 B.. Weight gain shockers slideshow: But where you gain weight may be related to menopause The weight gain may be most prominent around the face Im interested in the poster who mentioned using CoQ10 and restarting a period after menopause Now I am 48had a negative pap smear pelvic exam Chen and colleagues identified the major barriers to fertility preservation in transgender youth between the ages of 14 and 20 as cost invasiveness of procedures and reluctance to delay initiating hormone therapy for medical transition. An ovarian cyst may hemorrhage internally (cysts) of varying size ovary usu. Home , For Women , Menopause Flashes , Menopause Symptoms and of their symptoms of hot flashes and Very often when women are in a bad mood you will find that It is similar to a woman's menstrual cycle except that it only happens twice a year.. The Female Rat Reproductive Cycle: Follicles in virtually all stages of development are generally present within the ovaries at ...

To assess reduction in heavy menstrual bleeding and dysmenorrhea following MRI guided Focused Ultrasound Surgery (MRgFUS) of focal and diffuse adenomyosis up to 12 months post-treatment a retrospective cohort study was done at a tertiary care academic medical center for obstetrics, gynecology and infertility. MRgFUS for adenomyosis uterus was done for thirty-seven patients presenting with symptoms of heavy menstrual bleeding and dysmenorrhea with MRI-suspected adenomyosis. The main outcome measure, was reduction in heavy menstrual bleeding, dysmenorrhea and Symptom Severity Scoring (SSS) over a 3, 6 and 12 month period. Secondary outcome was evidence of fertility preservation post procedure. D'Agostino & Pearson omnibus normality test, one-way Ananova, Pearson's correlation coefficient analysis was performed on the data. Statistical significances, p-value and r-value were determined. Out of 37 patients who were treated by MRgFUS, 26 had sufficient follow-up to be included in the analysis. SSS calculated

Author: Schlesser Vincent, Hecq Jean-Daniel, Vanbeckbergen Danielle, Jamart Jacques, Galanti Laurence M, Year: 2002, Abstract: Cefepime is often used in antibiotic infusions for hospitalized patients. Using intravenous solutions of cefepime prepared in advance can be efficient, but little information is available about the stability of that antibiotic after freezing followed by microwave thawing. The purpose of this study was to investigate how freezing, long-term storage, and microwave thawing can affect the stability of cefepime in 5% dextrose injection.

The stability of 5 polyvinyl chloride bags of solu

Sugars are classified into three groups: monosaccharides, disaccharides, and trisaccharides. The simplest molecules of sugars are the monosaccharides, which include galactose, fructose and glucose, the only monosaccharides absorbed by humans. Disaccharides (including lactose, maltose and sucrose) and trisaccharides (including raffinose, found in cottonseed and sugar beets), are derived from the union of monosaccharides. All of these sugars provide approximately 4 cal/g.. Household 'sugar', or 'table sugar', is extracted mainly from sugar cane or beet. This sugar is a disaccharide composed of 50% glucose and 50% fructose linked by a-1,4 glycosidic bonds (Pancoast and Junk, 1980). Glucose, also known as dextrose or corn syrup, is produced from corn starch. Fructose is the sweetest of the simple sugars and is found as the monosaccharide, along with glucose and sucrose, in fruits and vegetables (Park and Yetley, 1993). It is generally present in honey and fruits and vegetables in similar amounts to ...

Male Infertility and IVF Treatment-Overview of Male Infertility- Male Infertility Specialist-Evaluation of Male Infertility-Male Fertility Problems-Cause,diagnosis and evaluation of Male Infertility-Male Fertility or Male Infertility-Treatment for Male Infertility -IUI, IVF, ICSI and Male Fertility Issues. From the medical standpoint, a couple is considered to be infertile if there has been no pregnancy after one to…