Reagents, Tools and Custom Services for molecular biology, specializing in the fields of Nano-Antibody development (nAb), Cellular Reprogramming (iPSC), Genome Editing, Fluorescent Proteins, RNAi, Viral Packaging and Protein expression.

One of the most remarkable chromatin remodelling processes occurs during spermiogenesis, the post-meiotic phase of sperm development during which histones are replaced with sperm-specific protamines to repackage the genome into the highly compact chromatin structure of mature sperm. Here we identify Chromodomain helicase DNA binding protein 5 (Chd5) as a master regulator of the histone-to-protamine chromatin remodelling process. Chd5 deficiency leads to defective sperm chromatin compaction and male infertility in mice, mirroring the observation of low CHD5 expression in testes of infertile men. Chd5 orchestrates a cascade of molecular events required for histone removal and replacement, including histone 4 (H4) hyperacetylation, histone variant expression, nucleosome eviction and DNA damage repair. Chd5 deficiency also perturbs expression of transition proteins (Tnp1/Tnp2) and protamines (Prm1/2). These findings define Chd5 as a multi-faceted mediator of histone-to-protamine replacement and ...

Complete information for CHD8 gene (Protein Coding), Chromodomain Helicase DNA Binding Protein 8, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Complete information for CHD9 gene (Protein Coding), Chromodomain Helicase DNA Binding Protein 9, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

CHD1 - CHD1 (untagged)-Human chromodomain helicase DNA binding protein 1 (CHD1) available for purchase from OriGene - Your Gene Company.

Next-day shipping cDNA ORF clones derived from chd7 chromodomain helicase DNA binding protein 7 available at GenScript, starting from $99.00.

A vast number of cancer genes are transcription factors that drive tumorigenesis as oncogenic fusion proteins. Although the direct targeting of transcription factors remains challenging, therapies aimed at oncogenic fusion proteins are attractive as potential treatments for cancer. There is particular interest in targeting the oncogenic PAX3-FOXO1 fusion transcription factor, which induces alveolar rhabdomyosarcoma (aRMS), an aggressive cancer of skeletal muscle cells for which patient outcomes remain dismal. In this work, we have defined the interactome of PAX3-FOXO1 and screened 60 candidate interactors using siRNA-mediated depletion to identify candidates that affect fusion protein activity in aRMS cells. We report that chromodomain helicase DNA binding protein 4 (CHD4), an ATP-dependent chromatin remodeler, acts as crucial coregulator of PAX3-FOXO1 activity. CHD4 interacts with PAX3-FOXO1 via short DNA fragments. Together, they bind to regulatory regions of PAX3-FOXO1 target genes. Gene ...

The mission of the CHARGE Syndrome Foundation is to provide support to individuals with CHARGE and their families; to gather, develop, maintain and distribute information about CHARGE syndrome; and to promote awareness and research regarding its cause and management.

The mission of the CHARGE Syndrome Foundation is to provide support to individuals with CHARGE and their families; to gather, develop, maintain and distribute information about CHARGE syndrome; and to promote awareness and research regarding its cause and management.

The etiology of CHARGE syndrome was unknown. We identified twin girls with CHARGE syndrome and a de novo apparently balanced chromosome translocation 46,XX,t(8;13)(q11.2;q22). By mapping the chromosome translocation breakpoints we found that the gene chromodomain-helicase-DNA-binding protein 7 (CHD7) located at 8q12 was disrupted in these girls. CHD7 has a genomic length of 188kb with 9000 coding bases over 37 exons. It has a putative function as a transcription factor which makes it a good candidate gene for a condition which affects multiple body systems ...

Parents of children with CHARGE should be encouraged to become IN CHARGE and very active advocates for their children in order to ensure that an educational program is made that will allow each child to reach their full potential. All children regardless of their final cognitive abilities will require special support in schools to ensure that they maximize their potentials and develop into the most productive people that they can be. In an educational setting all involved must be aware of the special needs a child with CHARGE may have. Teachers of children with CHARGE Syndrome have to be aware of all areas affected by the disease. Because CHARGE can affect the eyes, ears, and brain it is most important that all members of the educational team (teacher of the deaf and hard of hearing, teachers of the visually impaired, audiologists, pediatricians, parents, etc.) Taking each of these into account is vital to the success of the child and family in an educational setting. ...

CHARGE syndrome is a genetic disorder characterized by a specific and a recognizable pattern of anomalies, namely, coloboma, heart defects, atresia of choana, retardation of growth and/or...

References for Abcams Recombinant Human CHD4 protein (ab114276). Please let us know if you have used this product in your publication

I have a 8 year old son with CHARGE Syndrome. In my wanderings through the medical problems, finding answers and just wondering why, I found that there isnt much infomation out there for parents of children with CHARGE. So, in doing this page I have put together some of the information that I have gathered. Hopefully I have put together some things that I have found helpful. The information here is not just for parents of children with CHARGE, but can be used by anyone that has anything to do with a child with disabilities. Following is a short description of CHARGE Syndrome and a description of my sons problems. There is also a list of some of the places that I have found to be informational and helpful. ...

The mechanisms underlying the neurodevelopmental deficits associated with CHARGE syndrome, which include cerebellar hypoplasia, developmental delay, coordination problems, and autistic features, have not been identified. CHARGE syndrome has been associated with mutations in the gene encoding the ATP-dependent chromatin remodeler CHD7. CHD7 is expressed in neural stem and progenitor cells, but its role in neurogenesis during brain development remains unknown. Here we have shown that deletion of ...

The Senses Australia Girls Club was formed to foster friendships among young women with Deafblindness, while learning skills of hair and makeup. The purpose of the group was to bring together a group of young women with Deafblindness, and develop social skills over a shared activity. Participants in the group experience significant isolation in their everyday lives, some finding true friendship for the first time as young adults. These young women all have additional developmental disabilities, including CHARGE syndrome and epilepsy, requiring specialised support to meet the complexity of their learning needs. The intention of the Girls Club was to further support building of connections among the group of friends, while participating in an age appropriate activity, therefore reducing the experience of isolation felt by these young women ...

Human CHD3 partial ORF ( NP_001005273, 1654 a.a. - 1741 a.a.) recombinant protein with GST-tag at N-terminal. (H00001107-Q01) - Products - Abnova

Evergreen Sprinkler Systems, Inc. is a full-service irrigation installation and repair company providing service to the Florida counties of Miami-Dade and Monroe. Some examples of these products are wifi controllers, rain sensors, soil moisture systems, sprinklers, irrigation controllers, valves, and valve boxes. 7 months ago J&J did an amazing job!! Watering needs are different for lawns than they are for shrubs or trees. A drip system is installed above ground so it is an easier process with minimal disturbance. Download instructions for Snip-n-Spray Garden and Landscape Sprinkler System as a PDF Articles. Call us today at (501) 730-4515 to schedule sprinkler system installation or repair! 1105 S Lakeside Dr All the information you need to create a sprinkler system design for your lawn, shrubs or garden is in this landscape sprinkler design manual. We utilize proficient evaluation gear to trim and edge your property and keep your grass manicured and looking extraordinary. Water is the key to a ...

CHARGE syndrome is a condition that can disturb numerous areas of human body. As an abbreviation CHARGE stands for: coloboma, heart defects, atresia choanae, and retardation of growth, genital, and ear abnormalities. The configuration of malformations differs among individuals with this disorder, and the various health issues can be life-threatening during infancy and childhood. Affected individuals typically have several main features or a combination of major and minor appearances. Here we are presenting a case report of a neonate with CHARGE syndrome who underwent successful repair of choanal atresia under general anaesthesia with invasive monitoring.

Almost all children with CHARGE Syndrome have ear abnormalities. Many will have deformed outer ears that appear cupped. Middle and inner ear abnormalities occur frequently as well. In about 80-85% of children, hearing loss is prevalent. Establishing and maintaining balance may be a problem for some children.. Children with CHARGE syndrome usually have a number of different abnormalities. In addition to the findings that give the conditions its name, there are other problems that can frequently be seen in individuals with CHARGE syndrome. Some of these include postnatal growth problems, cleft lip and/or palate, immunity problems, facial paralysis, seizures, difficulties swallowing, abnormalities of the pituitary gland, tracheoesophageal fistula (an abnormal connection between the trachea or wind pipe and the esophagus or food pipe), and tracheosophageal atresia (the esophagus ends in a pouch instead of connecting to the stomach).. Once the major medical problems have been addressed, there can be ...

Baby Matt was born last September 10, 2017 with a rare condition known as CHARGE Syndrome and still fighting the condition in Pedia Intensive Care Unit(PICU) at Philippine General Hospital. CHARGE syndrome affects hearing, sight, breathing, feeding, heart function, and general development needs. Matt requires specialized care for all of these areas and medication might take years. A few minutes after Matt was born, he was intubated. He was later diagnosed to have a blocked nasal track on both nose( Bilateral Choanal Atresia), humming heart(suspected severe open heart condition), hydrocephalus, di george syndrome, physical deformities( ears and toes), too small genital, very low hemoglobin level and severe Pneumonia. My husband and I felt so bad knowing about his situation. We keep on asking ourselves what went wrong, what have we done, eat, etc... that might have caused this conditions, what have we done or have failed to do. We were so worried about our first born and felt so much guilt and ...

Dr. Kim Blake is a professor of Pediatrics at Dalhousie University in Nova Scotia, Canada. She has been researching in CHARGE syndrome over the last 35 years and has published extensively. She has answered research questions concerning post-operative airway events, sleep apnea, bone health, cranial nerve abnormalities and gastrointestinal issues. In the last 10 years Dr. Blake has partnered with Dr. Jason Berman and they have developed a zebra-fish-model of CHARGE syndrome to answer further research questions. ...

CHARGE is a rare condition that can affect different parts of the body. The most common problems are with the eyes, ears, heart, nasal passages, feeding and growth - although the condition, and its severity, does vary from person to person.. The name CHARGE was first used in 1981 to refer to a newly recognised cluster of features seen in a number of children. Over the years, it has become clear that CHARGE is a Syndrome and at least one gene causing CHARGE Syndrome has been discovered. The letters in CHARGE were originally used to describe some of the typical features of the syndrome as follows:. Coloboma of the eye, (This is an eye deformity where part of the eye has failed to develop properly and is missing) Heart Defects, Atresia of the choanae, (This is a closure of one, or both, of the openings at the back of the nose.) Delay of growth and/or development, Genital and/or urinary abnormalities, and Ear Abnormalities and deafness. It estimated that 4 - 6% of the deafblind population are ...

This gene encodes a protein containing two chromodomains and an ATP-binding helicase domain that functions as a regulator of transcription. Mutations in this gene result in an array of development defects, including inner ear problems. Mice defective for this gene exhibit many of the clinical features of the CHARGE syndrome caused by mutations in the homologous gene in human. [provided by RefSeq, Sep 2015 ...

Comprehensive sequencing efforts have revealed the genomic landscapes of common forms of human cancer and ~ 140 driver genes have been identified, but not all of them have been extensively investigated. CHD1L (chromodomain helicase/ATPase DNA binding protein 1-like gene) or ALC1 (amplified in liver cancer 1) is a newly identified oncogene located at Chr1q21 and it is amplified in many solid tumors. Functional studies of CHD1L in hepatocellular carcinoma and other tumors strongly suggested that its oncogenic role in tumorigenesis is through unleashed cell proliferation, G1/S transition and inhibition of apoptosis. The underlying mechanisms of CHD1L activation may disrupt the cell death program via binding the apoptotic protein Nur77 or through activation of the AKT pathway by up-regulation of CHD1L-mediated target genes (e.g., ARHGEF9, SPOCK1 or TCTP). CHD1L is now considered to be a novel independent biomarker for progression, prognosis and survival in several solid tumors. The accumulated knowledge

A plethora of mutations in chromatin regulators in diverse human cancers is emerging, attesting to the pivotal role of chromatin dynamics in tumorigenesis. A recurrent theme is inactivation of the chromodomain helicase DNA-binding (CHD) family of proteins-ATP-dependent chromatin remodelers that govern the cellular machinerys access to DNA, thereby controlling fundamental processes, including transcription, proliferation, and DNA damage repair. This review highlights what is currently known about how genetic and epigenetic perturbation of CHD proteins and the pathways that they regulate set the stage for cancer, providing new insight for designing more effective anti-cancer therapies.. ...

Description: CHARGE syndrome was initially defined as a non-random association of anomalies (Coloboma, Heart defect, Atresia choanae, Retarded growth and development, Genital hypoplasia, Ear anomalies/deafness). In 1998, an expert group defined the major (the classical 4Cs: Choanal atresia, Coloboma, Characteristic ears and Cranial nerve anomalies) and minor criteria of CHARGE syndrome. Individuals with all four major characteristics or three major and three minor characteristics are highly likely to have CHARGE syndrome. In 2004 a Netherlands study (Vissers, LELM, et.al) found a gene (CHD7 on 8Q12) which was implicated in 2/3 of those tested. This gene is responsible for encoding a number of DNA protein which is esential when neural crest cells are being formed and migrating. A negative genetic test for changes in the CHD7 gene can still result in a diagnosis of CHARGE ...

title: CHD7 mutational analysis and clinical considerations for auditory rehabilitation in deaf patients with CHARGE syndrome., doi: 10.1371/journal.pone.0024511, category: Article

All organs in the body originate from relatively simple structures in the embryo. For example a simple epithelial tube, the neural tube, develops into the highly complex brain. The many forces and growth factors that act upon embryonic tissues are precisely coordinated to shape the morphogenesis of more complex structures. We are interested in understanding how signalling centres are established in the embryo and how signalling pathways are regulated during development. Current research projects in the lab primarily focus on the fibroblast growth factor (FGF) signalling pathway and our aim is to elucidate how deregulated FGF signalling results in birth defects and cellular malfunction. We are particulalrly interested in understanding the functions of the Sprouty genes, which encode FGF antagonists, Tbx1, a T-box transcription factor implicated in DiGeorge syndrome and Chd7, a chromatin remodeller, mutated in CHARGE syndrome. We are studying the role of these genes in the development of the ...

This weekend my family and I were blessed to attend our first Texas Chargers Retreat/Conference to learn more about CHARGE Syndrome and connect with other families sharing our journey. I am usually very quiet and reserved around new people, yet this weekend I talked to anyone that would listen. It was beautiful to be among so many that understand my frustrations, fears and constant fatigue. I didnt have to explain the moments when watching my child do something new left me speechless, they already knew. They were my people. Their ears were open. They helped us. They loved my child without hesitation and allowed me to love their children. It was by far the best experience of our journey. My heart is full. ...

Group 4 Review: You need to make both your Introduction and Development of the Cardiovascular System headings proper headings using the == signs either side. This development section otherwise is very well laid out and comprehensible. I like your use of video and the way you have done a week-by-week breakdown. The rest of your website is very well written and descriptive - Im especially impressed by the detail in the development sections, and how you manage to convey the information clearly. It might be helpful to see a few images or figures showing the breakdown of this development to break up the text a little, but your subheadings are very helpful. The CHARGE Syndrome section has a couple of issues with phrasing in the paragraph below the link, which you might wish to address. The end of your website appears unfinished, for example in human congenital heart diseases associated with Neural crest cells; research and animal models, more detail and editing is required. You also have ...

Group 4 Review: You need to make both your Introduction and Development of the Cardiovascular System headings proper headings using the == signs either side. This development section otherwise is very well laid out and comprehensible. I like your use of video and the way you have done a week-by-week breakdown. The rest of your website is very well written and descriptive - Im especially impressed by the detail in the development sections, and how you manage to convey the information clearly. It might be helpful to see a few images or figures showing the breakdown of this development to break up the text a little, but your subheadings are very helpful. The CHARGE Syndrome section has a couple of issues with phrasing in the paragraph below the link, which you might wish to address. The end of your website appears unfinished, for example in human congenital heart diseases associated with Neural crest cells; research and animal models, more detail and editing is required. You also have ...

Silva AP, Ryan DP, Galanty Y, Low JK, Vandevenne M, Jackson SP, Mackay JP. Journal of Biological Chemistry 291, 924-938. Chromodomain Helicase DNA-binding protein 4 (CHD4) is a chromatin-remodeling enzyme that has been reported to regulate DNA damage responses through its N-terminal region in a poly(ADP-ribose) polymerase dependent manner. We have identified and determined the structure of a stable domain (CHD4-N) in this N-terminal region.

This diagram (with thanks to Prof Nelly Pitteloud, CHUV) shows the schematic layout of the causes of Kallmann syndrome or congenital hypogonadotropic hypogonadism with the migration of GnRH neurones and some of the gene defects that are linked to the two conditions. Listed are 16 of the currently 25 known genes, defects…

Description: A polyclonal antibody for detection of CHD1 from Human, Mouse. This CHD1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CHD1 protein at amino acid sequence of 960- ...

What are chromosomal disorders with immune deficiency? These disorders occur when there are missing, extra, or irregular parts of a persons chromosomal DNA. When associated with immune deficiency, chromosomal disorders may be linked to Down syndrome, CHARGE syndrome, DiGeorge Syndrome, and Cornelia de Lange syndrome, abnormalities of chromosomes 8 or 18.

CHD8 (Chromodomain-Helicase-DNA binding protein 8) is a member of the chromodomain helicase DNA-binding (CHD) subfamily of enzymes, which also belongs to the SNF2 family of ATP-dependent chromatin remodelers ...

When I was in my late 20s I had a conversation at work with two female colleagues during which one of them asked if I wanted children. For some reason I said I cant have them. To which they replied How do you know that ?. I could not really answer that question. It is…

The reported incidence of CHARGE Syndrome ranges from 0.1 to 1.2/10,000 and depends primarily on professional recognition. It is not known to be related to any illness, exposure to drugs or alcohol intake during pregnancy, and typically it does not occur to more than one person in a family. It is very rare, and cannot be predicted. Coloboma mainly affects the retina. Major and minor congenital heart defects (commonest cyanotic heart defect is tetralogy of Fallot) occur in 75 80% of patients. Choanal atresia may be membranous or bony, bilateral or unilateral, and is present in 50 60 percent of cases. Mental retardation (ranging from minimal to profound retardation) is another common feature. Under-development of external genitalia is a common finding in males but is less apparent in females. Ear abnormalities include classical finding of unusually shaped ears and hearing loss (conductive and/or nerve deafness resulting mild to severe deafness).. The cause of CHARGE is not known. Mutations in CHD7 ...

AbstractBackground: The CHD5 gene located on 1p36 encodes a protein - chromodomain helicase DNA-binding protein 5. CHD5 has been shown to be a tumor suppressor gene candidate. This study investigated the involvement of CHD5 in ovarian cancer and its clinicopathological significance. Methods: CHD5 expression in ovarian cancer and its counterpart were determined by quantitative RT-PCR. The correlation of CHD5 expression to clinicopathological features of the tumor was analyzed. Results: CHD5 expression was downregulated by at least twofold in 32 of 72 (41%) invasive epithelial ovarian carcinomas when compared to 12 controls in Hong Kong Chinese women. CHD5 downregulation was correlated to clinical status (p < 0.05), but not to patient age, tumor type and grade, recurrence and clinical stage (p > 0.05). Survival analysis showed that patients with CHD5 downregulation in their tumors were associated with shorter disease-free and total survival times compared to those without CHD5 downregulation (p < ...

In order to maintain cellular viability and genetic integrity cells must respond quickly following the induction of cytotoxic double strand DNA breaks (DSB). This response requires a number of processes including stabilisation of the DSB, signalling of the break and repair. It is becoming increasingly apparent that one key step in this process is chromatin remodelling. Here we describe the chromodomain helicase DNA-binding protein (CHD4) as a target of ATM kinase. We show that ionising radiation (IR)-induced phosphorylation of CHD4 affects its intranuclear organization resulting in increased chromatin binding/retention. We also show assembly of phosphorylated CHD4 foci at sites of DNA damage, which might be required to fulfil its function in the regulation of DNA repair. Consistent with this, cells overexpressing a phospho-mutant version of CHD4 that cannot be phosphorylated by ATM fail to show enhanced chromatin retention after DSBs and display high rates of spontaneous damage. These results provide

In order to maintain cellular viability and genetic integrity cells must respond quickly following the induction of cytotoxic double strand DNA breaks (DSB). This response requires a number of processes including stabilisation of the DSB, signalling of the break and repair. It is becoming increasingly apparent that one key step in this process is chromatin remodelling. Here we describe the chromodomain helicase DNA-binding protein (CHD4) as a target of ATM kinase. We show that ionising radiation (IR)-induced phosphorylation of CHD4 affects its intranuclear organization resulting in increased chromatin binding/retention. We also show assembly of phosphorylated CHD4 foci at sites of DNA damage, which might be required to fulfil its function in the regulation of DNA repair. Consistent with this, cells overexpressing a phospho-mutant version of CHD4 that cannot be phosphorylated by ATM fail to show enhanced chromatin retention after DSBs and display high rates of spontaneous damage. These results provide

What is the RCM (Reverse Charge Mechanism) under GST? Reverse charge is a mechanism under which the recipient of the goods or services is liable to pay the tax

Oligodendrocyte precursor cells (OPCs) constitute the main proliferative cells in the adult brain, and deregulation of OPC proliferation-differentiation balance results in either glioma formation or defective adaptive (re)myelination. OPC differentiation requires significant genetic reprogramming implicating chromatin remodeling. Mounting evidence indicates that chromatin remodelers play important roles during normal development and their mutations are associated with neurodevelopmental defects, with CHD7 haploinsuficiency being the cause of CHARGE syndrome and CHD8 being one of the strongest Autism Spectrum Disorder (ASD) high-risk associated genes. Here, we report on uncharacterized functions of the chromatin remodelers Chd7 and Chd8 in OPCs. Their OPC-chromatin-binding profile combined with transcriptome and chromatin accessibility analyses of Chd7-deleted OPCs, demonstrates that Chd7 protects non-proliferative OPCs from apoptosis by chromatin-closing and transcriptional repression of p53.

Loss of the chromatin remodeling ATPase CHD5 has been linked to the progression of neuroblastoma tumors, yet the underlying mechanisms behind the tumor suppressor role of CHD5 are unknown. In this study, we purified the human CHD5 complex and found that CHD5 is a component of the full NuRD transcriptional repressor complex, which also contains methyl-CpG binding proteins and histone deacetylases. The CHD5/NuRD complex appears mutually exclusive with the related CHD4/NuRD complex as overexpression of CHD5 results in loss of the CHD4 protein in cells. Following a search for genes that are regulated by CHD5 in neuroblastoma cells, we found that CHD5 binds to and represses the G2/M checkpoint gene WEE1. Reintroduction of CHD5 into neuroblastoma cells represses WEE1 expression, demonstrating that CHD5 can function as a repressor in cells. A catalytically inactive mutant version of CHD5 is able to associate with a NuRD cofactor but fails to repress transcription. Our study shows that CHD5 is a ...

Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...

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Congenital. CHARGE Syndrome. Chapple Syndrome. VACTERL. ​. Stroke. ​. Infectious. Acute otitis media. Necrotizing otitis externa. Meningitis. HIV. Polio. ​. Trauma. Birth trauma. Temporal bone fracture. Blunt trauma. Penetrating trauma. Injury to or sacrifice of the facial nerve during surgery ...

Oxford handbook of cancer lung cancer quality of life scale, its symptoms sub-scale and all others to further psychosocial stressors or genetically predetermined factors, which give a % risk nexium plavix vs of endometrial cancer and hormone-replacement therapy in new-onset atrial fibrillation. Infertility is associated with beckwith-wiedemann syndrome, charge syndrome mutations most commonly affected and pain assessment in each of these probabilities can be estimated from the -year survival for a capacity assessment when the focus of evaluation, with the examination. Is the patients problem. Graphics can be given with prednisone, although there is no diagnostic value. Escape from control. Tolvaptan competitive vasopressin receptor antagonist. Equivalent oral glucocorticoid doses mg day has already occurred. Criteria for clinically localized disease develop a management plan with patient. Diamniotic twins intrauterine death at term, antenatal care monochorionic. The dose should be. Intelligence ...

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Stephen McMahon of the Irish Patients Association has said many people will not be able to afford a €50 charge that may be applied to medical cards ...