Paper microfluidic devices allow passive transport of fluids through capillary force, and carry intrinsic advantages such as low-cost, flexible, and simple fabrication and easiness to store, transport, and dispose. Liquid transport in paper depends on the structure and material of the pre-manufactured paper, and controlling the flow in a continuous and active manner appears rather difficult. This paper presents a novel concept for active control of the flow in paper using a centrifugal force by integrating a paper strip into a disc-shaped centrifugal microfluidic platform and rotating it with various spin speeds. The flow in the paper under different strength of the centrifugal force is characterized. The paper demonstrates the ability to control the flow rate in a programmable manner as well as reverse the flow direction. ...
F3088-14 Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions cell adhesion~ cell-to-material interaction~
Cytomegalovirus infection remains a major cause of morbidity and mortality in marrow transplant recipients. Results of a rapid centrifugation viral culture of bronchoalveolar lavage specimens from 33 marrow transplant recipients with pneumonia were compared with those for conventional viral culture of concurrently or subsequently obtained lung tissue. The centrifugation culture results were also compared to results of cytologic and immunochemical examination of these specimens. Centrifugation culture was positive within 16 hours of inoculation in 22 of 23 (96%) specimens from patients with positive conventional culture of lung tissue. Detection of cells positive for cytomegalovirus by immunofluorescent antibody staining or cytologic identification was less sensitive (59% and 29%, respectively). There was no evidence of cytomegalovirus in specimens from patients without evidence of cytomegalovirus pulmonary infection by any technique. The sensitivity (96%) and specificity (100%) of centrifugation ...
A prototype CFSM will be further developed to facilitate single-molecule manipulation. A sealed gasket encloses the sample chamber, which will be mounted on a spinning compact disk (see figure). A ds-DNA library, containing important transcription initiation and termination sequences, will be spotted on the lower surface of the sample chamber using DNA microarray technology. Each ds-DNA, prepared with biotin at its distal end, will bind a strepavidin-coated fluorescent microsphere. The centrifugal force will push (when viewed in a rotating frame of reference) these microspheres radially and so cause the attached ds-DNA to stretch. The laser on a modified compact disk player will be used to track the bead displacement. The centrifugal force on each microsphere can be calculated based on their size, density and radial velocity. The centrifugal force plotted against microsphere displacement will provide information about the physical properties (persistence length, overstretching transition, etc) ...
TY - GEN. T1 - Magnetic assistance for suppressing cross-reactions between biomolecules in immunoassay. AU - Hong, Chin Yih. AU - Yang, Shieh Yueh. AU - Horng, Herng Er. AU - Yang, Hong Chang. PY - 2010/12/1. Y1 - 2010/12/1. N2 - A method involving the use of magnetic nanoparticles to suppress the cross-reactions in immunoassay is developed. Antibodies are coated onto magnetic nanoparticles. These antibodies bind with target and non-target molecules. Once an alternative-current magnetic field is applied, magnetic nanoparticles oscillate with the magnetic field. The target and non-target molecules attached onto magnetic nanoparticles via antibodies experience a centrifugal force, which is against the association between antibodies and target/non-target molecules. Theoretically, the centrifugal force is proportional to the square of the frequency of the applied magnetic field. Thus, the strength of the centrifugal force can be manipulated by changing the frequency of the applied magnetic field. By ...
Thus, we see that the centripetal force acting on a body is always provided by some other type of force -- centripetal force, thus, is simply a name to indicate the force that provides this circular motion. This centripetal force is always acting inward toward the center. You will know this if you swing an object in a circular motion. If you notice carefully, you will see that you have to continuously pull inward. We know that an opposite force should exist for this centripetal force(by Newtons 3rd Law of Motion). This is the centrifugal force, which exists only if we study the body from a non-inertial frame of reference(an accelerating frame of reference, such as in circular motion). This is a so-called pseudo-force, which is used to make the Newtons law applicable to the person who is inside a non-inertial frame. e.g. If a driver suddenly turns the car to the left, you go towards the right side of the car because of centrifugal force. The centrifugal force is equal and opposite to the ...
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Centrifugation is the process which involves the separation of heterogeneous mixture by means of spinning and this involves the application of centripetal force for the separation of liquids-liquid and liquid-solid mixtures. Centrifugation works on the principle of sedimentation that can be achieved by rapid rotation. Centrifuges are the devices which are used in separation of genetic materials, virulent substances, blood cell components, and others. The next generation centrifuges provides precise temperature control, wide range of relative centrifugal force (RCF), acceleration, and time. All the aforementioned parameters must be maintained and achieved for greater separation of quality components. Continuous flow centrifuges functions at relatively higher speeds as compared to the normal centrifugation devices. Such devices are very well known for their capability to isolate particles similar in nature. Whereas conventional centrifugation process separate molecules in batches, liquid flows ...
Neuation offers a wide range of next generation Centrifuges for various Research lab applications. From personal centrifuge to Universal laboratory centrifuges the range of Micro centrifuges and Lab centrifuges are widely used in microbiology, tissue culture, molecular biology, clinical and blood banking, drug discovery, genomics and proteomics. Neuation products are designed and developed by it own R & D team. Employing a User Centric Design Process to develop simple, Safe, easy of use and high performance laboratory centrifuges. The products are CE certified and approved for IVD use.
Neuation offers a wide range of next generation Centrifuges for various Research lab applications. From personal centrifuge to Universal laboratory centrifuges the range of Micro centrifuges and Lab centrifuges are widely used in microbiology, tissue culture, molecular biology, clinical and blood banking, drug discovery, genomics and proteomics. Neuation products are designed and developed by it own R & D team. Employing a User Centric Design Process to develop simple, Safe, easy of use and high performance laboratory centrifuges. The products are CE certified and approved for IVD use.
It is true that centrifugal force on earth has its counterpart in centripetal force, in gravity, but it would be pure foolishness to presume that centrifugal force -- i.e., fleeing away from the center of mass -- holds the wheat-stalk upright, or allows trees to dance lithely in the air, or causes millions of other plants, grasses and climbing vines to writhe ecstatically toward the heavens. Human ingenuity cannot produce the miracle of the wheat-stalk, the spiralling morning glory vine, or a massive sequoia tree. In terms of engineering these are living design miracles that defy both gravity and mechanical law. Nothing constructed by human skill could be so supple and yet so stable. Constructed on terrestrial principles, the wheat stalk is nevertheless incapable of standing tall and steady due to terrestrial laws alone. It needs the co-emergence of Andromedan physics. The plant-world on earth mirrors the physics of beauty, the aesthetic laws that prevail on Andromeda. Van Goghs sunflowers grow ...
Define centrifugal. centrifugal synonyms, centrifugal pronunciation, centrifugal translation, English dictionary definition of centrifugal. adj. 1. Moving or directed away from a center or axis. 2. Operated by means of centrifugal force. 3. Physiology Transmitting nerve impulses away from the...
A device for analysis used for transferring a solution to a measurement spot 38 by a centrifugal force and reading in which a reaction liquid located at the measurement spot 38 is optically accessed. An operation cavity 30 and a receiving cavity 32 are arranged from the upstream side to the downstream side of the transfer. The operation cavity 30 and the receiving cavity 32 communicate with each other via a connection section 59 to transfer the solution of the operation cavity 30 to the receiving cavity 32. The connection section 59 is located inside the liquid level of a diluted solution retained in the operation cavity 30, relative to a rotation axis 102.
Mechanically Operated Centrifugal Switch - An electric switch that operates using the centrifugal force created from a rotating shaft. The switch is designed to activate or de-activate as a function of the rotational speed of the shaft. In single-phase, split-phase induction motors, the switch is used to disconnect the starting winding of the motor once the motor approaches its normal operating speed. The centrifugal switch consists of weights mounted to the shaft of the motor and held near the shaft by spring force. At rest, levers attached to the weights press a low-friction, non-conductive plate against a set of electrical contacts mounted to the motor housing, closing the contacts and connecting the starting winding to the power source. When the motor approaches its normal operating speed, centrifugal force overcomes the spring force and the weights swing out, raising the plate away from the electrical contacts. This allows the contacts to open and disconnects the starting winding from the ...
Synonyms for Centrifugal acceleration in Free Thesaurus. Antonyms for Centrifugal acceleration. 1 antonym for centrifugal force: centripetal force. What are synonyms for Centrifugal acceleration?
Centrifugation is the use of the centrifugal forces generated in a spinning rotor to separate biological particles, such as cells, viruses, sub‐cellular organelles, macromolecules (principally proteins and nucleic acids) and macromolecular complexes (such as ribonucleoproteins and lipoproteins)
Cyclones are not as efficient (99.5%) as bag filters but several can be placed in series. Air enters at tangent at high velocity into a cylinder or cone which has a much larger cross section. Air velocity is decreased in the cone permitting settling of solids by gravity. Centrifugal force is important in removing particles from the air stream. High air velocity is needed to separate small diameter and light materials from air; velocities may approach 100 ft/sec (70 MPH). Higher centrifugal force can be obtained by using small diameter cyclones, several of which may be placed in parallel; losses may range from 0.5-2%. A rotary airlock is used to remove powder from the cyclone. (An example of a rotary airlock is a revolving door at a hotel lobby which is intended to break the outside and inside environments).. ...
Coupling greases, unlike bearing or general purpose grease, must withstand the centrifugal forces created by a rotating coupling. These greases - KSG and KHP - are specifically formulated to resist the heavy centrifugal forces associated with all applications. These forces can cause the all-important base oil to separate from the soap thickeners and additives. Unlike greases with lithium-based thickeners, KSG and KHP greases use polyethylene thickeners, with a density closer to that of the base oil, and therefore much less susceptible to separation ...
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A centrifugal machine, also known as a centrifuge, is an important part of any hospital, health care or testing laboratory. These essential pieces of equipment are used daily to generate extremely high rotating speeds, creating a centrifugal force that forces materials of different densities to separate. Of course these materials do need to be in a suspension for the process to work, but they are perfect for separating the solids from the liquids and distinguishing between materials of different weights and densities within the suspension.
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Cell culture: monocultures. Primary human fetal astrocyte cultures were established from 3 different brains as previously described (67). We confirmed an identical in vitro phenotype of commercially available cells (Lonza) and cross-referenced these to previous phenotypes. To establish human CD3+ T lymphocyte cultures from human blood of healthy donors, density centrifugation using Ficoll-Paque PLUS (GE Healthcare) was initially performed to isolate peripheral blood mononuclear cells (PBMCs) from whole blood. Six milliliters of whole blood containing acid citrate dextrose anticoagulant (Biological Specialty Corp.) was diluted with an equal volume of HBSS (Mediatech Inc.) and carefully layered in 15-ml tubes prefilled with 4 ml of density gradient medium. Tubes were centrifuged 40 minutes at 900 relative centrifugal force (RCF). PBMCs were collected by transfer pipette and afterward washed 2-3 times in HBSS (Mediatech Inc.) and centrifuged 15 minutes at 250 RCF. Human CD3+ T lymphocytes were then ...
Cell culture: monocultures. Primary human fetal astrocyte cultures were established from 3 different brains as previously described (67). We confirmed an identical in vitro phenotype of commercially available cells (Lonza) and cross-referenced these to previous phenotypes. To establish human CD3+ T lymphocyte cultures from human blood of healthy donors, density centrifugation using Ficoll-Paque PLUS (GE Healthcare) was initially performed to isolate peripheral blood mononuclear cells (PBMCs) from whole blood. Six milliliters of whole blood containing acid citrate dextrose anticoagulant (Biological Specialty Corp.) was diluted with an equal volume of HBSS (Mediatech Inc.) and carefully layered in 15-ml tubes prefilled with 4 ml of density gradient medium. Tubes were centrifuged 40 minutes at 900 relative centrifugal force (RCF). PBMCs were collected by transfer pipette and afterward washed 2-3 times in HBSS (Mediatech Inc.) and centrifuged 15 minutes at 250 RCF. Human CD3+ T lymphocytes were then ...
Harvest cowpea plants about 2 weeks after inoculation, then homogenize at 4°C in two volumes of 0.5 M citrate buffer (pH 7.0) containing 0.1% thioglycollic acid. Express juice through cheesecloth, and add 20 ml carbon tetrachloride to every 100 ml extract. Shake the extract for 15 min, and clarify by low-speed centrifugation. Concentrate the virus by three cycles of differential centrifugation. Resuspend the pellets from high speed centrifugation in 0.01 M citrate buffer. Purify further by sucrose density-gradient centrifugation (Tsuchizaki et al., 1971).. ...
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Hello Kapil,. Thanks for your interesting questions.. Sorry for the delayed replay. I was swamped in my work for a while, needless to explain the pressure.. Answer to your 1st question: As we all know, speed vac or centrifugal evaporator works by reducing the pressure under vacuum so does the boiling point of a given solvent. The centrifugal force generated during this process will evaporate the solvent from top, thus it prevent loss or solute by solvent bumping (in other words, by violent boiling). During centrifugal evaporation of HCl from our sample, we all assume that there will be a small loss of hydroxy proline (HYP) but this will be a similar error if we treat all our samples in the same way. It is known that the loss of HYP on drying is ≤ 0.1% at 110 C and I am sure centrifugal evaporator with no heat (assuming we are not using heating option in the instrument) will not reach this temp.. In summary, you have to dry the HCl in the absence of heat in centrifugal evaporator. If you are ...
... , The operator should not leave the centrifuge until full operating speed is attained and the machine appears to be running safely without vibration. Stop the centrifuge immediately if an unusual condition (noise or vibration) begins and check load balances. After Centrifugation. Allow the centrifuge to come to a complete stop before opening.
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An evacuated tube having both ends closed has a centrifugally actuated slit type valve fixedly disposed between the ends for dividing the tube into upper and lower chambers. The valve is formed and arranged to provide a passageway between the upper and lower chambers when subjected to a centrifugal force of proper intensity and direction. Upon cessation of the force, the valve closes to provide a separation between the upper and lower chambers.
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Looking for plasma centrifuge? Find out information about plasma centrifuge. A device for separating isotopes in which a cylinder of ionized matter is contained by a magnetic field and set into rotation by application of an... Explanation of plasma centrifuge
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The most common density gradient centrifugation method uses Ficoll Paque®, a solution of high molecular weight sucrose polymers. A blood sample is centrifuged in a tube containing the density gradient medium to separate its components according to their densities. Erythrocytes have a higher density than plasma, and so become packed in the bottom of the tube to make up 45% of the total volume (in blood from healthy donors). This volume percentage is known as hematocrit. Leukocytes and platelets form a narrow buff-colored layer (buffy coat) immediately above the erythrocytes. Finally, plasma makes up just under 55% of the total volume and is a pale yellow fluid. The buffy coat contains lymphocytes, monocytes, and platelets and is harvested for two subsequent centrifugations in a buffered salt solution to wash the PBMCs and remove the platelets. The resulting PBMCs can be used for the isolation of cell subtypes or other experimental procedures. A protocol for the isolation of PBMCs from blood by ...
Allogeneic MSCs being used in current human clinical trials are nonclonal, i.e., such MSCs may have other types of cells in the final stem cell product. Some concerns exist about the heterogeneity of these nonclonal MSCs that are isolated and expanded by the conventional density-gradient centrifugation method. Recently, we developed a new protocol, called the subfractionation culturing method (SCM), to generate single-cell-derived clonal MSC (cMSC) lines from whole bone marrow aspirate without employing any centrifugation step for mononuclear cells and enzyme treatment process. This method allowed us to rapidly establish single-cell-derived human clonal MSC (hcMSC) lines from raw bone marrow aspirates and to establish a library of these hcMSC lines (Song et al., 2008).. The goal of this study is to evaluate the safety of allogeneic cMSCs established by the SCM and manufactured in GMP facility. This phase I clinical trial is a multicenter, single dose study of cMSC (1 x 10e6 cMSCs/Kg recipients ...
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MODEL RELEASED. Biological research. Researcher operating a high-speed centrifuge (cell separator) to separate a cell suspension into its constituent parts using centrifugal force. Photographed at the Genethon Laboratory, near Paris, France. - Stock Image G350/0506
DA-Cell solution enables sample preparation for multi- color flow cytometry and bypasses the need of centrifugation for washing suspension cells. The DA-Cell technology is designed to maintain samples in a 96 droplet based format instead of using a conventional microwell with walls (Figure 1B). DA-Cell is a centrifuge-less processing method using the unique wall-less features of the DropArray technology combined with a fully automated washer. The DA-Cell washer generates a laminar flow for each drop on the DA-Cell plate via dual-nozzle action, with one nozzle dispensing liquid into the drop and the other nozzle aspirating liquid. The DA-Cell workflow is superior over the traditional centrifugation method:. ...
To meet stringent limit-of-detection specifications for low abundance target molecules, a relatively large volume of plasma is needed for many blood-based clinical diagnostics. Conventional centrifugation methods for plasma separation are not suitable for on-site testing or bedside diagnostics. Here, we repo