Quantification of Endoglucanase Activity based on Carboxymethyl Cellulose in Four Fungi Isolated from an Aerated Lagoon in a Pulp and Paper Mill. Luis A. Ordaz-Díaz,a Juan A. Rojas-Contreras,b Felipe Flores-Vichi,δc Mónica Y. Flores-Villegas,a Carlos Álvarez-Álvarez,a Pryscila Velasco-Vázquez,a and Ana M. Bailón-Salas b,*. The aim of this study was to identify cellulolytic fungal strains capable of degrading cellulose from an aerated lagoon in a pulp and paper mill. Four fungal strains that were found to be highly active were isolated on carboxymethyl cellulose (CMC) and suggested to be CMCase/endoglucanase. The identified strains were Aspergillus niger, Penicillium sp.,Aspergillus fumigatus, and Mucor sp. All the strains were studied in terms of cultural morphological characteristics and microscopic examinations. The endoglucanase with the highest isolate production was Penicillium sp., which also showed the highest qualitative endoglucanase activity (1.3 cm), in addition to the main ...
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Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 × 108 spore/g (inoculum) with 125 μm of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), β-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40°C compared to their optimum activities conditions (60°C for FPase and 70°C for CMCase, β-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and β-glucosidase), xylanase and protease. Enzymatic ...
Results: It was observed that cellulase activity of T. reesei strains varies from 0.73 to 3.11 IU and T. reesei 5A shown maximum cellulase activity after 96 h of growth perod at 37 °C. Total genomic DNA was isolated from T. reesei 5A and then it was partially digested with Sau 3A and ligated to vector YEpFLAG-1, linearized with BamH1. The construct was used to transform Escherichia coli and recombinant clone(s) were screened on Reese medium supplemented with carboxymethyl cellulose and ampicillin. The E. coli recombinant clones were further confirmed by gene specific amplification using PCR. S. cerevisiae was transformed with the recombinant plasmids YEpFLAG-1-cel-exo and YEpFLAG-1-cel-endo isolated from E. coli transformants. Carboxymethyl cellulase (CMCase) activity was observed in three yeast transformants Tr-2, Tr-4 and Tr-6 as 0.50, 0.70 and 0.80 IU, respectively. no FPase (exoglucanse) activity was observed in any of the yeast transformants ...
Allcosmeticsource.com Cellulase 100,000u/g,1kg/bag,free shipping [EP170508014]- Cellulase 100,000u/g,1kg/bag,free shipping What is Cellulase 100,000u/g Cellulase refers to the total name of multiple enzymes which can catalyze and hydrolyze cellulose. Generally, cellulase which can hydrolyze natural cellulose contains three activity constituents: endoglucanase, exoglucanase and glucuroide. Function of Cellulase 100,000u/g (1) The effect of snowflake, stereoscopic and color brightness can be further improved (2)
Disclosed are improved methods for treating cotton-containing fabrics as well as the fabrics produced from these methods. In particular, the disclosed methods are directed to contacting cotton-containing fabrics with a cellulase solution containing a fungal cellulase composition which is substantially,free of all CBH I type cellulase components. Cotton-containing fabrics so treated possess decreased strength loss as compared to fabrics treated with a cellulase solution containing a complete cellulase composition.
Deletion of Cel48S from C. thermocellum led to a decrease in the enzymatic hydrolysis rate, a decrease in microbial hydrolysis rate, and a decrease in biomass formation during growth on Avicel.. The similarity of enzyme saturation curves for the WT and parent strains suggests that the ΔpyrF mutation in the parent strain has no effect on cellulosome function, as expected. The S mutant strain, however, exhibited a reduction in both specific activity and saturation rate. A reduction in specific activity is indicative of impaired function and consistent with decreased synergy among components of the cellulosome in the absence of Cel48S (3).. The role of GH families in cellulose solubilization is a topic of much debate. Family 48 cellulases are a prominent component of many bacterial cellulase systems and, due to their ubiquity, are thought to play an important role in cellulose solubilization (21). On one hand, disruption of the single family 9 GH in C. phytofermentans eliminated its ability to ...
Cellulases have numerous applications in several industries, including biofuel production, food and feed industry, brewing, pulp and paper, textile, laundry, and agriculture.Cellulose-degrading bacteria are widely spread in nature, being isolated from quite different environments. Cellulose degradation is the result of a synergic process between an endoglucanase, an exoglucanase and a,β-glucosidase. Bacterial endoglucanases degrade ß-1,4-glucan linkages of cellulose amorphous zones, meanwhile exoglucanases cleave the remaining oligosaccharide chains, originating cellobiose, which is hydrolyzed by ß-glucanases. Bacterial cellulases (EC 3.2.1.4) are comprised in fourteen Glycosil Hydrolase families. Several advantages, such as higher growth rates and genetic versatility, emphasize the suitability and advantages of bacterial cellulases over other sources for this group of enzymes. This review summarizes the main known cellulolytic bacteria and the best strategies to optimize their cellulase
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A novel hemicellulase-producing fungal strain was isolated from a local soil sample. The organism is identified as Aspergillus fumigatus based on ribosomal RNA analyses. The Aspergillus strain, designated as 2NB, produces both enzymes acting on xylan backbone (xylanase and β-xylosidase), and those acting on side chains (or accessory enzymes) notably α-arabinofuranosidase and acetyl-xylan esterase. The Asperigillus hemicellulases are characterized as having relatively low xylanase and β-xylosidase activities but high side chain removal activities. The activity ratio of side-chain acting enzymes to xylanase is higher than that of the Multifect enzyme, a commercial hemicellulase product. The potential of the novel hemicellulases in lignocelluloses bioprocessing was demonstrated with alkaline-pretreated switchgrass as lignocellulose substrate with hemicellulase supplemented with a ratio of xylanase activity to filter paper unit of 2:1. Supplement of Aspergillus hemicellulases to commercial ...
Utilization of cellulases as substitute of chemical process gained huge momentum in the field of biotechnology Now there is dire need to find out un explore reveres of fungi possessing greater potential for efficient cellulase production. This boosted isolation of novel thermo tolerant fungal strains capable of producing the targeted product. In this investigation 70 thermophilic cellulolytic fungal strains were isolated. All the strains were screened via submerged fermentation. The strain showing highest CMCase activity was identified by conventional method i.e. based on morphology and microscopic features and confirmed by 18S rDNA gene sequencing, using specific ITS primers. The modified CTAB method was used for rapid extraction of DNA from thermo tolerant strain. The selected strain subsequently subjected to sequencing and phylogenetic analysis. The result indicates the selected strain was found to be T. dupontii. For strain improvement the T. dupontii was subjected to random mutagenesis by ...
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The structures of the Glu140--|Gln mutant of the Clostridium thermocellum endoglucanase CelC in unliganded form (CelC(E140Q)) and in complex with
Several alkalophilic Bacillus spp. strains were selected for their capacity to produce alkaline cellulases. Culture supernatants of these strains showed optimal cellulase activities between pH 8 and 9 and they were stable from pH 6 to pH 12. A cellul
Summary of Facts and Submissions. I. The appeal lies from a decision of the Opposition Division revoking European patent 0 699 248, granted on European application No. 95 913 647.4.. II. The application as filed comprised 8 claims, reading, respectively, as follows:. 1. In a method for enhancing the feel and/or appearance and/or for providing color enhancement to a non-cotton containing cellulosic fabrics during manufacture of the fabric by treatment of the fabric with a composition comprising a naturally complete fungal cellulase composition which comprises exo-cellobiohydrolase type component(s) and endoglucanase type component(s) wherein the improvement comprises modifying the naturally complete fungal cellulase composition to comprise at least 10 weight percent of endoglucanase type components based on the total weight of protein in the fungal cellulase composition and be free of all CBH I type cellulase components.. 2. The method according to Claim 1 wherein said fungal cellulase ...
Non-cellulosomal processive endoglucanase 9I (Cel9I) from Clostridium thermocellum is a modular protein, consisting of a family-9 glycoside hydrolase (GH9) catalytic module and two family-3 carbohydrate-binding modules (CBM3c and CBM3b), separated by linker regions. GH9 does not show cellulase activity when expressed without CBM3c and CBM3b and the presence of the CBM3c was previously shown to be essential for endoglucanase activity. Physical reassociation of independently expressed GH9 and CBM3c modules (containing linker sequences) restored 60-70% of the intact Cel9I endocellulase activity. However, the mechanism responsible for recovery of activity remained unclear. In this work we independently expressed recombinant GH9 and CBM3c with and without their interconnecting linker in Escherichia coli. We crystallized and determined the molecular structure of the GH9/linker-CBM3c heterodimer at a resolution of 1.68 Å to understand the functional and structural importance of the mutual spatial orientation
Hyper-Productivity, Characterization, and Exploitation of a Cellulase Complex from a Novel Isolate of Aspergillus tubingenesis S2 using Lignocellulose-based Material
Effect of pH on the activity of (■) free cellulase, (●) immobilized cellulase, and (▲) immobilized cellulase + ionic liquid. Morphologies of immobilized c
Cellulase produced byTrichoderma viride acted on carboxymethyl cellulose with a Km of 4.9 g substrate per litre, showing a pH optimum at 4.5 and a temperature optimum at 55 °C. Ag+, Hg2+, Zn2+, Cu2+ and N3- were inhibitory.
The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut ...
glucosidases. A gene encoding endoglucanase, designated as cel12, was cloned from total RNA prepared from F. palustris grown at the expense of Avicel. The gene encoding Cel12 has an open reading frame of 732 bp, encoding a putative protein of 244 amino acid residues with a putative signal peptide residing at the first 18 amino acid residues of the N-terminus of the protein. Sequence analysis of Cel12 identified three consensus regions, which are highly conserved among fungal cellulases belonging to GH family 12. However, a cellulose-binding domain was not found in Cel12, like other GH family 12 fungal cellulases. Northern blot analysis showed a dramatic increase of cel12 mRNA levels in F. palustris cells cultivated on Avicel from the early to late stages of growth and the maintenance of a high level of expression in the late stage, suggesting that Cel12 takes a significant part in endoglucanase activity throughout the growth of F. palustris. Adventitious expression of cel12 in the yeast Pichia ...
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored...
The microbial degradation of cellulose and xylans requires several types of enzyme such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [(PUBMED:1886523)]. Structurally, cellulases and xylanases generally consist of a catalytic domain joined to a cellulose-binding domain (CBD) by a short linker sequence rich in proline and/or hydroxy-amino acids.. The CBD domain is found either at the N-terminal or at the C-terminal extremity of these enzymes. As it is shown in the following schematic representation, there are two conserved cysteines in this CBD domain - one at each extremity of the domain - which have been shown [(PUBMED:1761039)] to be involved in a disulphide bond. There are also four conserved tryptophan, two are involved in cellulose binding. The CBD of a number of bacterial cellulases has been shown to consist of about 105 amino acid residues [(PUBMED:1812490), (PUBMED:10973978)].. ...
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Hemicellulase is used to clarify the juice of mandarin oranges. Although pectinase is used to clarify the juices of apples and grapes, the juice of mandarin oranges are clarified by the simultaneous use of pectinase and hemicellulase. In food processing, it is rare that the desired result can be obtained with the use of hemicellulase alone. Hemicellulases work in conjunction with cellulases and pectinases ...
Paper sludge is the largest solid waste stream produced by pulp and paper industry, and is also an attractive feedstock for emergent technologies based on processing of cellulosic biomass featuring enzymatic hydrolysis. This study focuses on investigating conversion of paper sludge to ethanol under industrially relevant conditions. A solids-fed simultaneous saccharification and fermentation laboratory reactor system capable of aseptic, semi-continuous metered delivery of paper sludge was developed to carry out experiments with hydrolysis mediated by commercial cellulase preparations and fermentation of glucose to ethanol mediated by Saccharomyces cerevisiae. Economically recoverable concentrations of ethanol were produced, and good material balance closure was achieved. Decreasing feeding frequency (feed additions per residence time) was found to allow the cellulase loading to be decreased at least two-fold with no decrease in cellulose conversion. Although decreasing feeding frequency results ...
Paper sludge is the largest solid waste stream produced by pulp and paper industry, and is also an attractive feedstock for emergent technologies based on processing of cellulosic biomass featuring enzymatic hydrolysis. This study focuses on investigating conversion of paper sludge to ethanol under industrially relevant conditions. A solids-fed simultaneous saccharification and fermentation laboratory reactor system capable of aseptic, semi-continuous metered delivery of paper sludge was developed to carry out experiments with hydrolysis mediated by commercial cellulase preparations and fermentation of glucose to ethanol mediated by Saccharomyces cerevisiae. Economically recoverable concentrations of ethanol were produced, and good material balance closure was achieved. Decreasing feeding frequency (feed additions per residence time) was found to allow the cellulase loading to be decreased at least two-fold with no decrease in cellulose conversion. Although decreasing feeding frequency results ...
The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family D [3] or as the glycosyl hydrolases family 8 [4,E1]. The enzymes which are currently known to belong to this family are listed below. ...
Books and Book Chapters. 1. Lactase production by Aspergillis Oryzae , Lambert publications, Germany, 2012. 2. Cellulase production by Aspergillus niger , Lambert publications , Germany, 2012. 3. Biological synthesis, Characterization and antimicrobial activity of silver nanoparticles from beetle leaves, In Nanoscience and drugdelivery. Apple science publication USA. Projects:. 1. Major Research project on Cellulase production by mutant fungal strain Aspergillus niger Sanctioned by University Grants Commision 2009. New Delhi, India. Abstracts. Indian Science Abstracts:. ...
The present invention provides a novel cellulase composition obtainable from Bacillus sp. CBS 669.93. A preferred cellulase has a calculated molecular weight of approximately 63 kD, a calculated isoelectric point of about 5 and a pH optimum on CMC of about 6 at 40 C. and 60 C.
Cellualse is one of the most important enzymes used in textile, detergent, paper, food and feed industries. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples. 24 soil samples were analyzed and 54 presumptive Bacillus isolates were isolated after heating the soil samples at 80°C for 10 min. Among them 45 isolates showed enzyme activity ranging from 0.003 to 0.17 U/ml in test tubes containing 5 ml medium composed of (g/L) glucose 0.5 gm, peptone 0.75 gm, FeSO4 0.01 gm, KH2PO4 0.5 gm, and MgSO4 0.5 gm at 120 rpm, 37° C and pH 7. Among them 1RW, 2WS, 3YR, 4WT, 6 RR, and 9SS showed 0.17, 0.15, 0.14, 0.15, 0.147 and 0.14U/ml enzyme activities, respectively. Production of cellulase by these isolates was further scaled up to shake culture containing 50 ml medium similar to that used in test tube culture. Among the isolates 1 RW showed the maximum activity. This 1 RW was identified by API kit and showed that 59 % belongs to ...
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Omega Pharma Cellulase Anticellulite Mousse 150ml. Cellulase Crackling Mousse is a cellulite treatment that improves skin vasodilation by stimulating the microcirculation, improves penetration of active ingredients and provides a pleasant feeling of...
Cellulase is often a plant enzyme that aids during the digestion of fibrous substances. Cellulase is utilized as a digestive aid and for the management of flatulence.Cellulase Cellulase is surely an enzyme which breaks down cellulose to beta-glucose. Human beings will not make cellulase of their bodies, and so are therefore struggling to use a lot … Read More. ...
Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.
endo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.- ...
endo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.- ...
Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression
Spratling, B.M., R.D. Wiedmeier, D.R. ZoBell, C.A. Fitzgerald, and B.A. Kent. 2001. Exogenous cellulase enhances the utilization of ammoniated wheat straw in pregnant and lactating beef cows. Proceedings, Western Section, American Society of Animal Science. Vol. 52:349-352.. ...
Read "Expression and import of an active cellulase from a thermophilic bacterium into the chloroplast both in vitro and in vivo, Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related pol
Cellulase is any of several enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis, the decomposition of cellulose and of some related pol
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Produced primarily by fungi, bacteria, and protozoans to catalyze cellulolysis, or the decomposition of cellulose and its related polysaccharides.
Shell said it will pay Codexis $7.5m for the remaining full-time employee equivalents (FTEs) and milestone payments that would have been due under the original agreement. Shell also agreed not to sell any cellulase enzymes to third party biofuel customers using technology developed by Codexis after the end of the Shell Research deal.. Shell retains its right to use and manufacture the enzymes including those enzymes that result from Codexis development in the span of a ten-year period beginning August 31, 2012. The enzymes will be for Shells and its affiliates own use only. Shell can also sub-license the right to manufacture the enzymes to third parties but only for Shells own use.. Codexis, on the other hand, was able to get a royalty-bearing, non-exclusive license to develop, manufacture, use and sell cellulase enzymes for biofuels use worldwide (except in Brazil) developed during the companies research collaboration since November 1, 2006. Codexis, however, has the exclusive rights to ...
Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). According to sequence and protein domain analyses, EGLB was assigned to glycosyl hydrolase family 5 of the cellulase superfamily. Several binding sites were found in the promoter region. The purified recombinant enzyme was induced by 0.5% methanol, and it exhibited optimal activity at 70 °C and pH 4. EGLB was stable for 3 h at temperatures below 60 °C, with more than 90% of its activity remaining. The enzyme was specific for substrates with β-1,3 and β-1,4 linkages. In Lineweaver-Burk plot analysis, the Km and Vmax values of EGLB for β-D-glucan were 134 mg/mL and 4.68 U/min/mg, respectively. The enzyme activity was increased by 1.86-fold by Co2+ and by 2-fold by Triton X-100 and
WBGene00001198 not CELE_ in pgid 348 WBGene00001198 not CELE_ in pgid 350 WBGene00001198 not CELE_ in pgid 351 WBGene00001198 not CELE_ in pgid 352 WBGene00002126 not CELE_ in pgid 1424 WBGene00009821 not CELE_ in pgid 1520 WBGene00012263 not CELE_ in pgid 2914 WBGene00043408 not CELE_ in pgid 2914 WBGene00009175 not CELE_ in pgid 2914 WBGene00016878 not CELE_ in pgid 2914 WBGene00020512 not CELE_ in pgid 2914 WBGene00019252 not CELE_ in pgid 2914 WBGene00015732 not CELE_ in pgid 3033 WBGene00003454 not CELE_ in pgid 3330 WBGene00003440 not CELE_ in pgid 3330 WBGene00003441 not CELE_ in pgid 3330 WBGene00003427 not CELE_ in pgid 3330 WBGene00003461 not CELE_ in pgid 3330 WBGene00003459 not CELE_ in pgid 3330 WBGene00003428 not CELE_ in pgid 3330 WBGene00003447 not CELE_ in pgid 3330 WBGene00003455 not CELE_ in pgid 3330 WBGene00003453 not CELE_ in pgid 3330 WBGene00003436 not CELE_ in pgid 3330 WBGene00003439 not CELE_ in pgid 3330 WBGene00023572 not CELE_ in pgid 4733 WBGene00023572 not CELE_ ...
WBGene00001198 not CELE_ in pgid 348 WBGene00001198 not CELE_ in pgid 350 WBGene00001198 not CELE_ in pgid 351 WBGene00001198 not CELE_ in pgid 352 WBGene00002126 not CELE_ in pgid 1424 WBGene00009821 not CELE_ in pgid 1520 WBGene00012263 not CELE_ in pgid 2914 WBGene00043408 not CELE_ in pgid 2914 WBGene00009175 not CELE_ in pgid 2914 WBGene00016878 not CELE_ in pgid 2914 WBGene00020512 not CELE_ in pgid 2914 WBGene00019252 not CELE_ in pgid 2914 WBGene00015732 not CELE_ in pgid 3033 WBGene00003454 not CELE_ in pgid 3330 WBGene00003440 not CELE_ in pgid 3330 WBGene00003441 not CELE_ in pgid 3330 WBGene00003427 not CELE_ in pgid 3330 WBGene00003461 not CELE_ in pgid 3330 WBGene00003459 not CELE_ in pgid 3330 WBGene00003428 not CELE_ in pgid 3330 WBGene00003447 not CELE_ in pgid 3330 WBGene00003455 not CELE_ in pgid 3330 WBGene00003453 not CELE_ in pgid 3330 WBGene00003436 not CELE_ in pgid 3330 WBGene00003439 not CELE_ in pgid 3330 WBGene00023572 not CELE_ in pgid 4733 WBGene00023572 not CELE_ ...
General Information: Country: Russia; Environment: Hot spring; Isolation: Terrestrial neutral geothermal spring; Temp: Hyperthermophile; Temp: 79C. Caldicellulosiruptor hydrothermalis is a thermphilic gram positive bacterium. This organism produces a thermostable cellulase which may have industrial uses. ...
A method for preparing an aqueous solution enriched in EG III from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The method involves adding an amount of a low molecular weight alcohol selected from the group consisting of ethanol, methanol, propanol and mixtures thereof to the aqueous mixture containing cellulase proteins, xylanase and EG III and an organic salt under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The method then involves removing the precipitate from the aqueous mixture so as to recover an aqueous supernate enriched in EG III. Next, the method involves adding an amount of an inorganic salt to the supernate produced in step b) so as to form a second precipitate and a second supernate and then finally collecting the second supernate from the second precipitate to obtain a supernate enriched in EG III.