TY - JOUR. T1 - Multistep carcinogenesis and genital papillomavirus infection. Implications for diagnosis and vaccines. AU - Rangel, L. M.. AU - Ramirez, M.. AU - Torroella, M.. AU - Pedroza, A.. AU - Ibarra, V.. AU - Gariglio, P.. PY - 1994/1/1. Y1 - 1994/1/1. N2 - Activated cellular oncogenes (myc and ras, for example) and inactivated anti-oncogenes (p53 or Rb) participate in multistep carcinogenesis. In addition, some high risk human papillomaviruses (HPV) are also involved in uterine cervix carcinomas. Typification of HPV is important for clinical diagnosis. Unravelling the complexities of the immune system and understanding the biochemistry and molecular genetics of cellular oncogenes and tumor viruses have opened up new possibilities for vaccination.. AB - Activated cellular oncogenes (myc and ras, for example) and inactivated anti-oncogenes (p53 or Rb) participate in multistep carcinogenesis. In addition, some high risk human papillomaviruses (HPV) are also involved in uterine cervix ...

TY - JOUR. T1 - Tight coordination of protein translation and HSF1 activation supports the anabolic malignant state. AU - Santagata, Sandro. AU - Mendillo, Marc L.. AU - Tang, Yun Chi. AU - Subramanian, Aravind. AU - Perley, Casey C.. AU - Roche, Stéphane P.. AU - Wong, Bang. AU - Narayan, Rajiv. AU - Kwon, Hyoungtae. AU - Koeva, Martina. AU - Amon, Angelika. AU - Golub, Todd R.. AU - Porco, John A.. AU - Whitesell, Luke. AU - Lindquist, Susan. PY - 2013. Y1 - 2013. N2 - The ribosome is centrally situated to sense metabolic states, but whether its activity, in turn, coherently rewires transcriptional responses is unknown. Here, through integrated chemical-genetic analyses, we found that a dominant transcriptional effect of blocking protein translation in cancer cells was inactivation of heat shock factor 1 (HSF1), a multifaceted transcriptional regulator of the heat-shock response and many other cellular processes essential for anabolic metabolism, cellular proliferation, and tumorigenesis. ...

The potential role of arachidonic acid metabolism in the enhancement (promotion) of malignant transformation of C3H/M2 mouse fibroblasts by the tumor promoter 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was investigated using inhibitors of cyclooxygenase and lipoxygenase activities. The promoting effects of TCDD (1.5 pM) and of the reference tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA; 0.4 mM) on carcinogen (N-methyl-N′-nitro-N-nitrosoguanidine or 3-methylcholanthrene)-pre-treated fibroblasts was abolished by cotreatment with indomethacin, hydrocortisone, caffeic acid or nordihydroguaiaretic acid. A differential inhibition was found with N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide, a selective inhibitor of the cyclooxygenase isoenzyme COX-2: the promoting effect of TPA, but not that of TCDD, was abolished. Therefore, the role of the cyclooxygenase isoenzymes COX-1 and COX-2 during chronic exposure to TCDD was studied in more detail. Long-term treatment with TCDD (4-7 weeks) ...

Following a decade of planning and composition, Romanian troupe Dordeduh emerges from the cocoon a completely new and revitalized band.

Potentially involved in: lung cancer; cancer; neuroblastoma; melanoma; kidney cancer; carcinoma; skin cancer; allergic hypersensitivity disease; adenocarcinoma in situ; allergic rhinitis; alcoholic intoxication, chronic; brain neoplasms; non-small cell lung carcinoma; renal cell carcinoma; neoplastic cell transformation; mental depression; depressive disorder; granuloma, plasma cell; neoplasm metastasis; neoplasm recurrence, local. Disease data sourced from Pharos.. ...

Here we describe the isolation and characterization of rCop-1, a novel CCN family protein whose expression was completely lost after cell transformation. Functional studies suggest that rCop-1is a negative regulator for cell transformation based on the following findings. The loss of rCop-1 expression correlates extremely well with cell transformation in culture, since cells transformed by a variety of mechanisms all lost rCop-1 expression. BALB/c A31 and Rat-1 are both immortalized but nontransformed, yet only the former expresses rCop-1. However, both A31 and REFs, the parental cells of Rat-1, lose rCop-1 expression when transformed by a variety of means. This suggests that the loss of rCop-1expression in cultured rodent fibroblasts may not be necessary for cell immortalization but may be so for cell transformation. Efficient retroviral gene transfer of rCop-1 exhibited a dramatic cytotoxic effect on the transformed cells but had little effect on the nontransformed cells.. The first member of ...

TY - JOUR. T1 - The effect of malignant transformation on the sensitivity of murine fibroblasts to the antiviral effect of interferon. AU - Morris, A. G.. AU - Barrett, Alan. AU - Bird, R. M.. AU - Burke, D. C.. PY - 1979. Y1 - 1979. UR - http://www.scopus.com/inward/record.url?scp=0018286199&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018286199&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0018286199. VL - 6. SP - 139. EP - 141. JO - FEMS Microbiology Letters. JF - FEMS Microbiology Letters. SN - 0378-1097. IS - 3. ER - ...

Cancer is believed to be driven by genetic mutations. However, mouse models and human patients with cancer-predisposing germ line mutations show that only a small fraction of their cells become malignant, suggesting mutations themselves are not sufficient to cause transformation and additional determinants must exist. There are two prevailing, non-exclusive models to account for the additional determinants. One is that full transformation requires accumulation of multiple genetic mutations, which posits transformation occurs only when all mutations have been acquired. The recent success in reprogramming malignant cells into pluripotent stem cells indicates that normal cells of essentially all lineages can exist in the presence of replete cancer mutations. The second model postulates that mutations in rare stem cells initiate malignancy. Advancement in sequencing technology has led to the detection of cancer mutations in the hematopoietic stem cells (HSCs) of healthy individuals. The prevalence ...

The small GTPase RhoA controls many cytoskeletal processes, including actin polymerization, F‐actin bundling, myosin‐based contractility, formation of focal adhesions and formation of the contractile ring at cytokinesis (for a review see Van Aelst and DSouza‐Schorey, 1997). In addition, RhoA plays a less well understood role in the control of cell cycle progression, transformation and tumour cell invasiveness. In fibroblasts, activated RhoA is sufficient to induce DNA synthesis (Olson et al., 1995), and cooperates with activated Raf in focus formation assays (Khosravi‐Far et al., 1995; Qiu et al., 1995). Activated Rho GDP/GTP exchange factors (GEFs) are potent oncogenes in focus assays (for a review see Whitehead et al., 1997), and it has been proposed that their ability to induce anchorage‐independent growth arises because they mimic signalling by adhesion receptors (Schwartz et al., 1996). Rho activity is required for transformation by Ras and for induction of DNA synthesis in ...

This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patients clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician ...

Carcinogenesis is caused by a cumulative, multistage process that mainly consists of initiation, promotion, and progression. ROS, which are produced as a result of the metabolism of molecular oxygen via biochemical reactions in cells, play a key role in tumor promotion. Some tumor promoters accelerate/induce the conversion of initiated cells (carcinogen-mediated mutation or potential stem cells) into tumorigenic cells possibly via the production of oxidative/inflammatory responses (30, 31). TPA (12-O-tetradecanoylphorbol-13-acetate), a phorbol ester, is a tumor promoter that induces the neoplastic/tumorigenic transformation of preneoplastic JB6 cells through the overproduction of ROS (32). In this study, we investigated the inhibitory effect of SFN on TPA-stimulated neoplastic transformation in the mouse epidermal JB6 P+ cell line to assess whether SFN is able to block tumor promoter-induced tumorigenesis in skin cells. Our results show that SFN was effective when it was given together with ...

To find the next steps to digital transformation, bank executives should set aside the noise and consider some guidelines from early adopters.

A non-profit impact organization at the University of Michigan. We work to transform data and research into useful information that improves the health of people and communities.

The battleground of business today isnt won with a single magic bullet, like better tech or big data. Its won with cohesive, intelligent front and back office operations supporting best-in-class experiences. When executed upon properly, the transformative implications for your business are massive.. With tech, customer tastes and preferences, methodologies and even best practices changing so quickly, its no wonder that over 50% of the Fortune 500 will be out of business by 2025.. To keep up with this change, C-Suite and Executive Leadership expect their talent (including themselves) to acquire the latest and greatest knowledge, learning, training, and skills.. Becoming irrelevant. Its one of the things that keeps every CEO and founder up at night. And our MasterClass training is squarely aimed at mitigating that worry. ...

New parents, such as George and Christine, have helped to transform life since their arrival in August. Thanks to a parents steady presence, children dont spend their days roaming but rather creating, learning and growing. Theyve turned empty space into projects for the family, even building their own pigeon house. They have brought African village life - the very thing these children lost - into the family to help the children identify with the culture around them.. The boys especially embrace the presence of a father. George delights in teaching them his skills in masonry and painting, and puts them to good use in projects around the house - not to mention in the house, where hes taught them a thing or two about cooking! The boys wanted to share their newfound culinary skills by preparing a meal for a visitor : Horizons own Jack Eans, who gave his dinner two thumbs up.. ...

Wild (1995: 7) defines operations management in the following terms: Operations Management is concerned with the design and the operation of systems for manufacture, transport, supply or service. Stevenson (1999: 4) defines operations management as: the management of systems or processes that create goods and/or provide services. A transformation process is any activity or group of activities that takes one or more inputs, transforms and adds value to them, and provides outputs for customers or clients. Where the inputs are raw materials, it is relatively easy to identify the transformation involved, as when milk is transformed into cheese and butter. Where the inputs are information or people, the nature of the transformation may be less obvious. For example, a hospital transforms ill patients (the input) into healthy patients (the output).. ...

Anyone could give me some idea where I can find a good protocol for the transformation assay using Rat2 cells. The one you look for transformed foci. Do I need to stain the cells? Alex ...

The aim of this work was to develop a method to evaluate the kinetics of bainite transformation by theoretical deduction and thermal dilatation curve analysis. A Gleeble-3500 thermomechanical simulator and dilatometer (DIL805A) were employed to study the isothermal transformation in deformed (360 ∘ C , 600 ∘ C , and 860 ∘ C ) and undeformed conditions. The thermal dilatation information during isothermal transformation was recorded, and the dilatation curves were well smoothed. By taking a derivative of the dilation curve with respect to the transformation time, the peak time of transformation rate (PTTR) was obtained, which can serve as the essence of isothermal transformation time. The relative change of length ( Δ L / L ) due to phase transformation was theoretically deduced, and the effect of temperature was taken into consideration. Combing experimental data, the volume fraction of bainite in isothermal transformation was calculated. Making a graph of

1) the conversion of a normal cell into a cell that is able to divide indefinitely in culture, thus behaving like a cancer cell; (malignant transformation may also describe the series of changes in a normal cell in an organism that change it into a malignant (cancerous) cell); (2) a change in genotype and phenotype due to the assimilation of external DNA by a cell; when the external DNA is from a member of a different species, transformation results in horizontal gene ...

Image analyzing apparatus and methods are disclosed for analyzing 3-dimensional as well as 2-dimensional images. The pixels of the 3-D images may be represented by multivalued digital data signals which are analyzed in one or more programmable neighborhood transformation stages. In the preferred embodiment, each stage is programmed with selected contribution values associated with each pixel in the neighborhood. The values of the data signals for each pixel are modified by these contribution values and the maximum value thereof is selected as the transformation output of the stage. A series of dilation/erosion transformations may be used to transform the original image matrix in such a manner so as to locate the position and/or identify the shape of particular objects contained in the original image.

ACs top 3 transformation tips - After my recent 7 week transformation, many people have been asking me for tips on achieving a rapid transformation in muscle size and fat loss. There are no single tips that will ensure success in everyone as it totally depends on the person as to how good the results will be. However there are some helpful tips that I can offer that will help ensure success in anyone wanting to make a body transformation. So here are my top 3 tips: 1. Plan the full transformation before you start. With any transformation I do with a client I plan literally every workout before we even start. I do this on a spreadsheet that can be updated and adapted as time goes on. However the main thing is to know exactly what you are doing every day of every week. This will keep you incredibly focused and you will know what to expect from training each week. It also makes it incredibly easy to progress with performance and strength even whilst dropping fat. You will record every weight you use on

Finally, we tested whether IKKε/TBK1 is required for Ras transformation in MEFs. Active K-Ras was expressed in IKKε/TBK1 double-knockout cells that were reconstituted with TBK1 or vector. The IKKε/TBK1 double-knockout MEFs could not be transformed by active K-Ras (V12). In contrast, re-expression of TBK1, which restored AKT activation (Fig. S2B) but was insufficient to transform MEFs alone, supported a robust transformation by K-Ras (V12), as indicated by focus formation (Fig. 6C). These observations indicate an obligatory role of IKKε/TBK1 in Ras-induced oncogenic transformation of MEF cells. Future studies are needed to determine whether AKT activation is the major downstream effector of TBK1 supporting Ras-induced transformation.. As a major signaling hub, AKT regulates a wide range of cellular processes from cell growth, proliferation, to apoptosis (2). Pathological AKT activation is observed in many cancers and clearly plays a major role in tumorigenesis (1). It has been well ...

Findings from the CEO/Innovators Roundtable: Reform, Transition and Transformation Are at Hand等来自罗盛咨询的文章为您提供真知灼见，帮助您从容应对棘手的企业挑战。阅读更多。

For example, when designing the 90 Day Bikini Transformation Challenge I provided an exact calorie and macro breakdown specific to that persons body. Fitness trackers. Body Transformation shouldnt be hard, so weve taken all the proven science and created easy to follow body transformation programs, so that no matter when you start, or what are your challenges, well always be here to keep you accountable and help you reach your goals. Its about effort. Body Transformation: Jade Socobys Powerlifting Journey; Body Transformation: Marcin Maly Gets Shredded In 4 Months; Body Transformation: Sandi Holder Joined A Gym And Lost A Whopping 80lbs! Discreet packaging available. Advertisement. 24 Amazing Female Body Transformations Top 5. 14. Behind those smiles and fresh gym kit, theres an inspiring story to be told about how they embarked on their journey to a healthier self. POST #56 3 Month Of Hard Work. I like his tattoo. 1. The sample plan below was created by Shannon Dey, M.S., founder and CEO ...

Victoria Vesna and Siddharth RamakrishnanMETAMORPHOSIS OF THE HUMAN ANIMAL: HOX ZODIAC. The Homeobox (hox) genes essentially define body regions in all animals including humans - responsible for determining two arms, two legs, one nose and so on. This gene is shared by all living beings - from the snail to the elephant to humans - and it can now be manipulated into transforming certain parts of the body into others. We have observed such transformations, such as that of an amputated antenna into a limb, as far back as 1901, termed neomorphosis, and it has only recently re-emerged as an area of scientific study. Spontaneous transformations and induced regenerations are fascinating research topics that are fast becoming a reality; some scientists are postulating that it may be possible that the hox gene could be central to limb regeneration in the future.. This paper will present the Hox Zodiac project, which attempts to introduce this subject and push the ideas further into speculation of ...

Furthermore, the company is pleased to announce Ruy Pinto as the successor to Martin Halliwell as Chief Technology Officer (CTO). Ruy Pinto, formerly Deputy Chief Technology Officer and Chief Information Officer of SES will lead SESs global technology organisation including all technology functions currently within the business units and will also retain the leadership of SESs IT and Digital Transformation activities. Martin Halliwell will remain part of the Senior Leadership Team and serve as a Strategic Advisor to the CEO until his planned retirement in May 2019.. I am delighted to bring together our engineering talent and responsibility for our customer services under such strong and experienced leaders. As the demands of our markets evolve, these two teams, configured to serve our customers across both business units, will ensure that we stay ahead, says Steve Collar, President and CEO of SES. Unifying the technical team and creating a consistent customer facing organisation will allow ...

COX-1 and -2 gene expression can be regulated at both the transcriptional and the posttranscriptional levels, and species differences may exist at both levels. Transcription induced in murine or chicken embryo fibroblasts by mitogens or by oncogene-induced transformation occurs rapidly, and maximal levels are seen at 15-30 min, followed shortly by COX-2 mRNA and protein […] ...

The same transformation can work with an even modulo with the addition of a and shrink the compare RHS by the same amount Unless the target supports that transformation probably isn t worthwhile The transformation can also easily be made to work with non zero equality for ...

owwmenu image=A549_web.jpg font=arial, helvetica, sans-serif bold=1 color=blue bgcolor=Cornsilk hovercolor=lightcyan bghovercolor=rosybrown topFontSize=15 fontSize=10 pagewidth=800 lab=Cell Transformation Group, Home=#, Home=http://openwetware.org/wiki/Cell_Transformation_Group Members Research=#,Ongoing=Ongoing_Research Protocols=#,Cell_Culture=Protocols/Cell Culture,Protein=Protocols/Protein,RNA_and_DNA=Protocols/RNA and DNA Resources=#,Cell Lines=Resources/Cell Lines,Virus_Stocks=Resources/Virus Stocks,Glycerols=Resources/Glycerols,Antibodies=Resources/Antibodies Links=#, Sponsors=Links/Sponsors ,/owwmenu ...

Meet eight real people who lost weight and blogged about it. Plus, get diet and fitness tips and see photos of their amazing transformations at WomansDay.com.

The core of this is the ability to change faster by adopting newer methodologies and delivering higher quality. Cloud, mobile, social, and big data have been the driving technologies behind digital transformation at most companies worldwide. In addition, digital initiatives are driving higher spend on quality.. As digital transformation gains momentum, the need for speed-to-market is felt severely by both business and IT. To achieve that pace and flexibility, organizations are adopting agile methods and DevOps principles. In addition, there is an emerging need to have testing teams shift-left and be well-integrated with development teams to ensure that that enterprise agility is demonstrated in every aspect.. ...

We need to better understand the genetic mechanisms that transform normal blood cells into acute myeloid leukaemia (AML). Overall survival is improving for children and adults with AML, but traditional therapies can be ...

Vol 5: OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

The world is experiencing an unprecedented crisis that is causing chaos in the global economy, disrupting supply chains, and transforming society. The new reality is accelerating business model transformation at a faster pace than ever before to ensure existential survival in a crisis for which no one was prepared.

Meaningful technologies to help teams at healthcares defining moments. We work as trusted partners to our customers, helping them to adapt to healthcares evolving world and to advance digital health transformation at defining moments in a patients journey.

This is a book about joy and how to get it and keep it in our lives. It is all simpler than we are making it out to be, meaning living in our bodies. Its the small steps we take, day in and day out, that make a huge difference. Thats what creates lasting change and transformation at any age. We ju

The results presented here were not predicted on the basis of previous epidemiologic and functional studies of Trop2, all of which pointed to a gain-of-function role for this protein in the development of aggressive cancer and tumor susceptibility (2-7, 11, 23, 32-34). As such, we expected that Trop2 ablation would hinder cancer development. Instead, through the use of a novel Trop2 knockout mouse model generated in our laboratory, we uncovered an unpredicted relationship between Trop2 loss and cancer development. In addition, we were able to confirm a relationship between Trop2 loss and an increased progression towards EMT during epithelial transformation. The epidemiologic analysis presented in this report is the first description, to our knowledge, of the association between Trop2 loss and EMT in any primary human tumor and highlights the clinical relevance of our findings. Collectively, these observations reveal a far more complex functional role for this protein in the multistep development ...

During a recent presentation about the mass transition to Zoom/online-based classes in Spring 2020, the term digital transformation was thrown around. Now, admittedly, it was a very short presentation - 20 minutes - and encompassed the experiences of 3 institutions/systems to boot. So the presenters did not dig deep. But the clear implication by some was that by going online, teaching (and learning) was transformed. There were comments about how the intense crucible (my words) of switching to online in the spring necessitated transformation.. I…dont know how I feel about this. First, transformation is thrown around in a lot of industries, and higher education is no exception. Second, transformation is about changing how you go about doing your work, not just morphing it from one form to another. As someone working with faculty (I am one step removed, however, from direct interaction), I regularly see and hear about courses that were airlifted from in-person design to virtual settings. ...

BACKGROUND: In medical research, explanatory continuous variables are frequently transformed or converted into categorical variables. If the coding is unknown, many tests can be used to identify the optimal transformation. This common process, involving the problems of multiple testing, requires a correction of the significance level. Liquet and Commenges proposed an asymptotic correction of significance level in the context of generalized linear models (GLM) (Liquet and Commenges, Stat Probab Lett 71:33-38, 2005). This procedure has been developed for dichotomous and Box-Cox transformations. Furthermore, Liquet and Riou suggested the use of resampling methods to estimate the significance level for transformations into categorical variables with more than two levels (Liquet and Riou, BMC Med Res Methodol 13:75, 2013). RESULTS: CPMCGLM provides to users both methods of p-value adjustment. Futhermore, they are available for a large set of transformations. This paper aims to provide insight the user

Description. A Career with Hang Seng Bank. Hang Seng is committed to service excellence. Our people are our most important asset and play a vital role in our efforts to continually enhance our performance for customers and provide best-in-class products and services.. We seek to attract high-calibre talent by offering a dynamic working environment, good career development opportunities and competitive compensation packages.. Head of Transformation. Digital Business Services. Hang Sengs Transformation under Digital Business Services is a team of professionals dedicated to design and deliver a portfolio of bank-wide, large-scale transformation programmes and strategic change initiatives.. We are currently seeking a high caliber professional to join our department as Head of Transformation.. Principal responsibilities. ...

Lemotte, P K.; Adelstein, S J.; and Little, J B., Malignant transformation induced by incorporated radionuclides in balb/3t3 mouse embryo fibroblasts. (1982). Subject Strain Bibliography 1982. 54 ...

In the last chapter, characteristics and mechanisms of the early stages of neoplastic develop- ment-initiation and promotion-were presented as being the result of cellular and molecular changes induced by specific ...

Transformation can be deliberate and powerful, advancing your mission and your librarys status in the community. Every part of our lives has been transformed by the pandemic, but it is important to remember that not all transformation is beyond our control. There are ways to make deliberate transformations, but you need to be clear and…

Sometimes the observations for a variable are not immediately suitable for analysis and instead need to be transformed using a mathematical function. Transformations are often used to normalise the distribution of a variable, but can be used to change the scale, offset the observations or even recode groups.. Excel cell formulas and custom VBA functions can be used to transform or calculate the data for a variable. Transformed and calculated data is treated just like any other variable as far as measurement scale and observation precision are concerned. Excel functions most commonly used for transformations are:. ...

This report examines the Digital Transformation market standing and opportunity of global plus major regions, from plans of manufacturers, regions, product types and end industries; this report investigates the best manufacturers in global and major regions and splits the Digital Transformation market by product type and applications/end industries. The Global Digital Transformation market is considered…

When XSL transformation with Xalan involves too many temporary result trees, the transformation can run out of DTM IDs, resulting in an exception like ...

Social Transformation through Dynamic Education - We believe that it is through the spread of education in all the strata and sections of society that real social transformation can be brought about.. ...

Financial Institutions are continuously evolving to meet demand created by various drivers within the industry. These transformations are often the key to a businesss ability to thrive in the industry.

again. State-of-the-art research in genomics has revealed the startling fact that many diseases are caused by certain subsequences of bases not forming a palindromic sequence! Your mission as a leading researcher at ICPC laboratories is to take a DNA string $S$ and a series of subsets $P_1 , \ldots , P_ t$ of indices to characters (nucleobases) in $S$, and transform $S$ so that each of the restrictions of the resulting string to $P_1 , \ldots , P_ t$ are palindromic. (The restriction of $S$ to a subset $P = \{ i_1 , i_2 , \ldots , i_ k \} $ of indices, where $0 \leq i_1 , i_2 , \ldots , i_ k , ,S,$, is the string $S_{i_1} S_{i_2} \ldots S_{i_ k}$ ). It is possible to inspect any base of $S$ at will, but only three transformations can be applied to a base:. ...

Use expert tips to make your business effective and efficient.. Going through a digital transformation can be a daunting task. 1Datagroups offers business advisory services that will help you decide on the best course of action for your organization and its development.. It doesnt matter if you are using an old piece of software or looking to build something completely new and unique, we will help you find the best solution.. ...